LOCUS       DAZKSR010000001        89057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000001 DAZKSR010000000
VERSION     DAZKSR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.001"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..886
                     /locus_tag="ACG8XG_000001"
                     /pseudo
     CDS             324..886
                     /locus_tag="ACG8XG_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(1091..1429)
                     /locus_tag="ACG8XG_000002"
     CDS             complement(1091..1429)
                     /locus_tag="ACG8XG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0771953.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            1716..1946
                     /locus_tag="ACG8XG_000003"
     CDS             1716..1946
                     /locus_tag="ACG8XG_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0771954.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1946..2287
                     /locus_tag="ACG8XG_000004"
     CDS             1946..2287
                     /locus_tag="ACG8XG_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0771955.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            3137..3322
                     /locus_tag="ACG8XG_000005"
     CDS             3137..3322
                     /locus_tag="ACG8XG_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0771956.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            3827..4021
                     /locus_tag="ACG8XG_000006"
     CDS             3827..4021
                     /locus_tag="ACG8XG_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0771957.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            4212..4461
                     /locus_tag="ACG8XG_000007"
                     /pseudo
     CDS             4212..4461
                     /locus_tag="ACG8XG_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4705..5535)
                     /locus_tag="ACG8XG_000008"
     CDS             complement(4705..5535)
                     /locus_tag="ACG8XG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0771958.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            5743..7077
                     /locus_tag="ACG8XG_000009"
     CDS             5743..7077
                     /locus_tag="ACG8XG_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0771959.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            7118..8029
                     /locus_tag="ACG8XG_000010"
     CDS             7118..8029
                     /locus_tag="ACG8XG_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0771960.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            8026..8880
                     /locus_tag="ACG8XG_000011"
     CDS             8026..8880
                     /locus_tag="ACG8XG_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0771961.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            8877..9494
                     /locus_tag="ACG8XG_000012"
     CDS             8877..9494
                     /locus_tag="ACG8XG_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0771962.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            9496..10014
                     /locus_tag="ACG8XG_000013"
     CDS             9496..10014
                     /locus_tag="ACG8XG_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0771963.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            10312..10770
                     /locus_tag="ACG8XG_000014"
     CDS             10312..10770
                     /locus_tag="ACG8XG_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0771964.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            10879..14820
                     /locus_tag="ACG8XG_000015"
     CDS             10879..14820
                     /locus_tag="ACG8XG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0771965.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            14820..16133
                     /locus_tag="ACG8XG_000016"
     CDS             14820..16133
                     /locus_tag="ACG8XG_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0771966.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            16126..17823
                     /locus_tag="ACG8XG_000017"
     CDS             16126..17823
                     /locus_tag="ACG8XG_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0771967.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            17897..18790
                     /locus_tag="ACG8XG_000018"
     CDS             17897..18790
                     /locus_tag="ACG8XG_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0771968.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            18974..19648
                     /locus_tag="ACG8XG_000019"
     CDS             18974..19648
                     /locus_tag="ACG8XG_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0771969.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            19666..20691
                     /locus_tag="ACG8XG_000020"
     CDS             19666..20691
                     /locus_tag="ACG8XG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0771970.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            20780..21646
                     /locus_tag="ACG8XG_000021"
     CDS             20780..21646
                     /locus_tag="ACG8XG_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0771971.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            21683..23059
                     /locus_tag="ACG8XG_000022"
     CDS             21683..23059
                     /locus_tag="ACG8XG_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0771972.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(23160..24356)
                     /locus_tag="ACG8XG_000023"
     CDS             complement(23160..24356)
                     /locus_tag="ACG8XG_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0771973.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(24480..25142)
                     /locus_tag="ACG8XG_000024"
     CDS             complement(24480..25142)
                     /locus_tag="ACG8XG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0771974.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            25370..25933
                     /locus_tag="ACG8XG_000025"
     CDS             25370..25933
                     /locus_tag="ACG8XG_000025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0771975.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            25946..26230
                     /gene="macP"
                     /locus_tag="ACG8XG_000026"
     CDS             25946..26230
                     /gene="macP"
                     /locus_tag="ACG8XG_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0771976.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            26249..26941
                     /locus_tag="ACG8XG_000027"
     CDS             26249..26941
                     /locus_tag="ACG8XG_000027"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0771977.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            26953..27498
                     /locus_tag="ACG8XG_000028"
     CDS             26953..27498
                     /locus_tag="ACG8XG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0771978.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            27769..31047
                     /locus_tag="ACG8XG_000029"
     CDS             27769..31047
                     /locus_tag="ACG8XG_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0771979.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(31588..33495)
                     /locus_tag="ACG8XG_000030"
     CDS             complement(31588..33495)
                     /locus_tag="ACG8XG_000030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0771980.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            33625..34500
                     /locus_tag="ACG8XG_000031"
     CDS             33625..34500
                     /locus_tag="ACG8XG_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0771981.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            34580..35554
                     /locus_tag="ACG8XG_000032"
     CDS             34580..35554
                     /locus_tag="ACG8XG_000032"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0771982.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            35734..36879
                     /locus_tag="ACG8XG_000033"
     CDS             35734..36879
                     /locus_tag="ACG8XG_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0771983.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            37016..37360
                     /locus_tag="ACG8XG_000034"
     CDS             37016..37360
                     /locus_tag="ACG8XG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0771984.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            37631..38917
                     /gene="efmA"
                     /locus_tag="ACG8XG_000035"
     CDS             37631..38917
                     /gene="efmA"
                     /locus_tag="ACG8XG_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0771985.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39055..39654)
                     /locus_tag="ACG8XG_000036"
     CDS             complement(39055..39654)
                     /locus_tag="ACG8XG_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0771986.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            40058..41179
                     /gene="mnmA"
                     /locus_tag="ACG8XG_000037"
     CDS             40058..41179
                     /gene="mnmA"
                     /locus_tag="ACG8XG_000037"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0771987.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            41425..42846
                     /gene="arcD"
                     /locus_tag="ACG8XG_000038"
     CDS             41425..42846
                     /gene="arcD"
                     /locus_tag="ACG8XG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0771988.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            42860..43978
                     /locus_tag="ACG8XG_000039"
     CDS             42860..43978
                     /locus_tag="ACG8XG_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0771989.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(44038..45330)
                     /locus_tag="ACG8XG_000040"
     CDS             complement(44038..45330)
                     /locus_tag="ACG8XG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0771990.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            45480..46040
                     /locus_tag="ACG8XG_000041"
     CDS             45480..46040
                     /locus_tag="ACG8XG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0771991.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            46169..46546
                     /locus_tag="ACG8XG_000042"
     CDS             46169..46546
                     /locus_tag="ACG8XG_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0771992.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            46479..48551
                     /locus_tag="ACG8XG_000043"
     CDS             46479..48551
                     /locus_tag="ACG8XG_000043"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0771993.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(48762..49709)
                     /locus_tag="ACG8XG_000044"
     CDS             complement(48762..49709)
                     /locus_tag="ACG8XG_000044"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0771994.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            49955..50419
                     /locus_tag="ACG8XG_000045"
     CDS             49955..50419
                     /locus_tag="ACG8XG_000045"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0771995.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(50481..51524)
                     /locus_tag="ACG8XG_000046"
     CDS             complement(50481..51524)
                     /locus_tag="ACG8XG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0771996.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            51875..52738
                     /locus_tag="ACG8XG_000047"
     CDS             51875..52738
                     /locus_tag="ACG8XG_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0771997.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(52867..54489)
                     /locus_tag="ACG8XG_000048"
     CDS             complement(52867..54489)
                     /locus_tag="ACG8XG_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0771998.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            54738..55259
                     /locus_tag="ACG8XG_000049"
     CDS             54738..55259
                     /locus_tag="ACG8XG_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0771999.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(55445..57175)
                     /gene="spxB"
                     /locus_tag="ACG8XG_000050"
     CDS             complement(55445..57175)
                     /gene="spxB"
                     /locus_tag="ACG8XG_000050"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0772000.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            57340..58158
                     /locus_tag="ACG8XG_000051"
     CDS             57340..58158
                     /locus_tag="ACG8XG_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0772001.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(58261..58458)
                     /locus_tag="ACG8XG_000052"
     CDS             complement(58261..58458)
                     /locus_tag="ACG8XG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772002.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            58627..59205
                     /locus_tag="ACG8XG_000053"
     CDS             58627..59205
                     /locus_tag="ACG8XG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0772003.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            59300..59437
                     /locus_tag="ACG8XG_000054"
     CDS             59300..59437
                     /locus_tag="ACG8XG_000054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772004.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(60250..60753)
                     /locus_tag="ACG8XG_000055"
     CDS             complement(60250..60753)
                     /locus_tag="ACG8XG_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0772005.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            61112..62137
                     /locus_tag="ACG8XG_000056"
     CDS             61112..62137
                     /locus_tag="ACG8XG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0772006.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            62460..63338
                     /locus_tag="ACG8XG_000057"
     CDS             62460..63338
                     /locus_tag="ACG8XG_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0772007.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            63814..63930
                     /locus_tag="ACG8XG_000058"
     CDS             63814..63930
                     /locus_tag="ACG8XG_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0772008.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            64858..65196
                     /locus_tag="ACG8XG_000059"
     CDS             64858..65196
                     /locus_tag="ACG8XG_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772009.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            65290..65520
                     /locus_tag="ACG8XG_000060"
     CDS             65290..65520
                     /locus_tag="ACG8XG_000060"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0772010.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            65920..66291
                     /locus_tag="ACG8XG_000061"
     CDS             65920..66291
                     /locus_tag="ACG8XG_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0772011.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(66565..67257)
                     /locus_tag="ACG8XG_000062"
     CDS             complement(66565..67257)
                     /locus_tag="ACG8XG_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0772012.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(67260..68045)
                     /locus_tag="ACG8XG_000063"
     CDS             complement(67260..68045)
                     /locus_tag="ACG8XG_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0772013.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            68282..69682
                     /gene="lacG"
                     /locus_tag="ACG8XG_000064"
     CDS             68282..69682
                     /gene="lacG"
                     /locus_tag="ACG8XG_000064"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0772014.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            69707..70024
                     /locus_tag="ACG8XG_000065"
     CDS             69707..70024
                     /locus_tag="ACG8XG_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0772015.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            70037..71710
                     /locus_tag="ACG8XG_000066"
     CDS             70037..71710
                     /locus_tag="ACG8XG_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0772016.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            71724..73355
                     /locus_tag="ACG8XG_000067"
     CDS             71724..73355
                     /locus_tag="ACG8XG_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0772017.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(73846..75411)
                     /gene="guaA"
                     /locus_tag="ACG8XG_000068"
     CDS             complement(73846..75411)
                     /gene="guaA"
                     /locus_tag="ACG8XG_000068"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0772018.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(75470..76393)
                     /gene="coaA"
                     /locus_tag="ACG8XG_000069"
     CDS             complement(75470..76393)
                     /gene="coaA"
                     /locus_tag="ACG8XG_000069"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0772019.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            76589..77194
                     /locus_tag="ACG8XG_000070"
     CDS             76589..77194
                     /locus_tag="ACG8XG_000070"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0772020.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            77502..78518
                     /locus_tag="ACG8XG_000071"
     CDS             77502..78518
                     /locus_tag="ACG8XG_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772021.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            78659..79960
                     /locus_tag="ACG8XG_000072"
     CDS             78659..79960
                     /locus_tag="ACG8XG_000072"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0772022.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            80006..80668
                     /gene="deoC"
                     /locus_tag="ACG8XG_000073"
     CDS             80006..80668
                     /gene="deoC"
                     /locus_tag="ACG8XG_000073"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0772023.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            80689..81087
                     /locus_tag="ACG8XG_000074"
     CDS             80689..81087
                     /locus_tag="ACG8XG_000074"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0772024.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            81153..82235
                     /locus_tag="ACG8XG_000075"
     CDS             81153..82235
                     /locus_tag="ACG8XG_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0772025.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            82447..84009
                     /locus_tag="ACG8XG_000076"
     CDS             82447..84009
                     /locus_tag="ACG8XG_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772026.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            84002..85135
                     /locus_tag="ACG8XG_000077"
     CDS             84002..85135
                     /locus_tag="ACG8XG_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772027.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            85135..86091
                     /locus_tag="ACG8XG_000078"
     CDS             85135..86091
                     /locus_tag="ACG8XG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772028.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            86190..87356
                     /gene="deoB"
                     /locus_tag="ACG8XG_000079"
     CDS             86190..87356
                     /gene="deoB"
                     /locus_tag="ACG8XG_000079"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0772029.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            87380..88198
                     /locus_tag="ACG8XG_000080"
     CDS             87380..88198
                     /locus_tag="ACG8XG_000080"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0772030.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            88215..88922
                     /gene="deoD"
                     /locus_tag="ACG8XG_000081"
     CDS             88215..88922
                     /gene="deoD"
                     /locus_tag="ACG8XG_000081"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0772031.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        28286 a        15650 c        18845 g        26276 t
ORIGIN      
        1 taagaaaaat tttgtgaaaa tagctttcta catttttgca taaatttggc ttaataccga
       61 gaaactggct tttgaacacc gtttacccag aggtcgtaag cctgccgttt cactccgagt
      121 cacaaggaac aatttcaaaa agcagcttct catgtttgtt gaatattgtg acttgtgacc
      181 gaggagttgg caggagaata ccattcactg gagagtttgt cacttgtcac aactttttta
      241 gttctacaaa aattgaaagc gttttgttgg tgcgaacttc ttgttgcagt gtatgataga
      301 aaaaaagatt gggagggatt aggatgtgtg gtcgttattt ttttgatcta gagtcagagc
      361 tccttcagaa ttattatcga gaagcccaga cgaaacaacc gaagcaggca gcagaattag
      421 cagctggaga agtttttccg tcaaatcttg ttgtgacgtt aagaaaagag gaagaaaaca
      481 tcctcactcc ggaaatcatg aagtggggat ttaccggatt taaaaaagga caattgatga
      541 ttaatgcccg tgcagagaca gtagaagaaa aaaagacctt tcatcaacca tttttgcaaa
      601 atcgttgtgt tttcccaatg agtggaatga agaaaaagac aaatttatgt tttcaaataa
      661 ggaaaaaata gtgtatattg gcggttttta tcgtagccat caggatgaaa aaatggcaga
      721 atcaatcata atgactactg aacctaaccg cacagtcgca ccaatccatg accgaatgcc
      781 gcttgtcttg actgaagaac aaatcgaacc ttgggtaaca gatatttctt ttgcgcgaaa
      841 aatcattact cagcagatgc ctgaattagt aatggagaag gtataacata atcgattttt
      901 caaagaaaga gcggcaaaaa acgaaaaaga cttcagcaca gatcccaaaa acaaagagaa
      961 cagaagaaat gctcataagc tgcatatact gcatctatag ggtcacagtc gacctttcta
     1021 atgtatagct tctatttgtt ttttactcgt gctgcgagtc ttttttattt atacctaacg
     1081 atgtttatca ttatgactct ttgaattctt ggaatctaaa attcaaagtt aacgactggt
     1141 tggcagttgt atattctctg aatttataga taagaggttg ccatttatcc acattgatat
     1201 gagttggatc atgtaaaatc ccttcttcta caaaaatagc tacgatctca cgttctacaa
     1261 tagcaaagtc ttgtctagtt gaaattcgtt gaaccaatgt ttctatttga agcgggcatt
     1321 ctttgatccg aacagttgaa acttcttcac ctgctagttt cgtgaacttt cccaattgaa
     1381 atttgtcaga gcaaaaggaa tagccagctt tttgtttata atcgggcatg ataggatggc
     1441 ctgttgtctt tgaaattttc tcccaaagcg ttttatctgc aaaattgaag gtagcggggg
     1501 gttagctata gtatttgggt agcttttgga tccataggtt caatgtttct tggattattt
     1561 ttttagttaa agaaaaaatg aactggctac aatttatttc tatttatatc caataagata
     1621 tactttaaac aaaacaattt tctagatagt ttacaaaggg gaattttgcg ttacttagtg
     1681 ttatatagta tacttttatt aagggagtga tgataatgct tgttacaaaa tctagattac
     1741 aaggaagttc tgtagtggta actttacctt ctgataacgg aaagaaacct tcagaaaacc
     1801 aagaatatat cgttgtgtat tctgatgatg gaacaattac gttagttcca aaaattgaag
     1861 atccatttag tggtggggaa gaggccgagt attatgagaa agacgaatgg gaagatttaa
     1921 ctcctgaagg gagagaaatt ttatagtgtc agaagaaaga atttatattc ctaaaaaagg
     1981 tgatattgta tggattgatt ttgatccctc cgtaggaaaa gaaattcaaa aaagacgacc
     2041 aggactagtc gtctctcgtt atgaatttaa ccgtaaaaca atgttttcgg tcatatgtcc
     2101 aattacttcc actataaaga atttaccgac acgttactca ttacctgaag agctagatat
     2161 aaaaggacaa gtaattatta cacaattaaa atctcttgat tttaaggaac gaaaactcaa
     2221 aaaaatagaa aacttaccat tgcaagacat ggctaaaatt gatcagatta tacagtatat
     2281 gttttaagca ttgtaagtaa actctataat atttttatat gctaaacata acgaaattaa
     2341 aaaaaggcca agtaaatgtc gttcattcta ctagtgtggt cgatggcatt aatttccgta
     2401 tcttaaacta aagaatatat agcattttaa acgaatgatt gaggtgattt cctcaagtca
     2461 ttcgtttttt tgtgtcacaa aacttgtttt ttcacgacat atcttaacgt atacgaaaag
     2521 tcgcgacaat actttaacag aaagaagaaa aagaacaact gacagtttta gtcctaaaaa
     2581 atctaaactc tttagatatg agtaatgtca ttttaaacta tatcgatgct caaaaacgta
     2641 aaatttggga tcaagatctg tctaggaaag aatttctata gtttaactga ataacacaaa
     2701 aaggcatatc atgcgacaag aaaaacaatg aaaagagaaa tttgccatca ctggaacatc
     2761 catacagaaa ttagcataga ttctgaaaaa tctaccgcta gtttttattt agttgttcta
     2821 acgacaccaa tacttgtaat gatcgaaaat gtgcaattgg gtcagatagt gaaccaaagt
     2881 agaatctcaa agcgacaatt atgaatgtaa aagtaccttt tatgattagt ttattctcta
     2941 attgattaca tcccgtataa ttaataaaat tactttaccg attacacgat cttatgtcag
     3001 tagagctttt aggtacacta tcaatacagt tcattccagt ataatgtaag gtttttttta
     3061 ttttgagtca ctttttgtca atattattat ttacaatacc ttctataata aaagttattt
     3121 aaggaagtgt ctatgaatga gtaaaaattc taaaggattc ctaaccatct tattagcttt
     3181 tatagggtat atgttggttg ggctgctaaa atcatacagt aatgagttgc tgaatttttc
     3241 aacatttatt aatgatacat tagttccaag tttgtttttc attgtatttt ttgctgttgg
     3301 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
     3361 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
     3421 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
     3481 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
     3541 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
     3601 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
     3661 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
     3721 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
     3781 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
     3841 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
     3901 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
     3961 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
     4021 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
     4081 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
     4141 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
     4201 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
     4261 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     4321 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     4381 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     4441 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     4501 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     4561 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     4621 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     4681 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     4741 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     4801 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     4861 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     4921 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     4981 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     5041 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     5101 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     5161 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     5221 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     5281 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     5341 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     5401 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     5461 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     5521 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     5581 gaaagattaa aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa
     5641 taattttttt agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat
     5701 attaaaaccg gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt
     5761 acagggttgc tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca
     5821 agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat
     5881 attgatttct ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt
     5941 gaggatttca atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt
     6001 gacatctgga ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta
     6061 caggacataa attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa
     6121 tatgtagaaa aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat
     6181 gaaggtgatc cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa
     6241 aagttattta aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta
     6301 gaagaaacag cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt
     6361 tatccattat ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt
     6421 tggtttgaca aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag
     6481 tggatccaag actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa
     6541 tttgggtctg gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat
     6601 atcaattatt actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg
     6661 cagctcccag aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg
     6721 gaagtaccat acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct
     6781 tctccagaag tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca
     6841 gccaatgaga atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa
     6901 atggcggacg aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat
     6961 tacacgacat tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa
     7021 gaaggattgg cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc
     7081 atttttgact gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa
     7141 gaaaaaagaa gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt
     7201 atttatgcta gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac
     7261 atccaagatg aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt
     7321 attttggaaa tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac
     7381 gattttcagt gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt
     7441 ccgtacagct ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat
     7501 gcagctgctt tctccatctg caggattgat caacacattt ctgggctggt tccatatccc
     7561 tggaccagct tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat
     7621 atggagttct ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc
     7681 tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac
     7741 tttgccatta atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt
     7801 ccaaattttt caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc
     7861 tttgcttttt tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta
     7921 tgcgatggct atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt
     7981 gaagcttgca cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc
     8041 aaaaagacca gtgtgaatcg gcaaacaaaa aaaatactaa gttatgtcct catgacagta
     8101 ataggaatca ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg
     8161 gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat
     8221 ctggatacga ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact
     8281 gtgctagtag tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag
     8341 cttgattttc ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg
     8401 gggtttgtaa cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact
     8461 tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt
     8521 caattttact tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat
     8581 catttgtata tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt
     8641 gcgatcaatt ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa
     8701 gatcaagaaa aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca
     8761 caatggaatt atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc
     8821 ttttttgcac aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga
     8881 tgaatcgatc gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc
     8941 cacgtcttat ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag
     9001 gaactgcaag tcgaacagtt ctgtatatga tttatcatta caaaaaaaat gaagagaaaa
     9061 agctcggttc actattctgg aaaaagtttc gtgagaactt ctctctttat ggaaagcaag
     9121 atagtttagc aagtgtttat tttcttcttc tattgatcga ttatcagatt ttccgctatt
     9181 ggggtggggg aattggctat acattgatgt atactacgtt gtttttgctt ctattaagcg
     9241 cgcttctctt tagttacaaa atcttgcttc aactggaaca aaaagaagct tcttggatta
     9301 ctgcattttt cttgtttttt aatgatttca aaagcgcgct tttctatttg gcaggcacgt
     9361 tgatccttct tgtcgcattc tggtttgcag gaccaatcta ttttgcttta ttaggtttca
     9421 gtttcttgtt ttattttcaa gtcctgcttt ttatccatcg tacgcaagaa aaggttctta
     9481 aaaaggagga ataacatgtt gcgtttgatt gctaaaatcg atgatagtat tatcaacaag
     9541 ttcgccaagc aacggaaaac ggatagtcga aaaatcaatt ttttgaaatt tatcagtaat
     9601 atctctaatg gtggttttat ttggttgctc cttgctgctg ttttagtctt gatacctgat
     9661 actagagaat atggtctagc cattcttatc ggcagtttga tctatttttt tgttggtttg
     9721 ctttttatca aaaaaacgac aagacggaaa agaccttatg aaaaaaatcg tcggcttgtc
     9781 ctttacacaa gtaaaccgaa gacttcttct tttccttcag gacatacagc gctagccttt
     9841 ttatcagtag ctgcgttgtt cgaattaagt ccagttgcag gaatattagc ctttttattt
     9901 gcttgtttga tcgcttattc gaggattttt ttacttttac attatcctac tgacatttta
     9961 ggaggagcta ttttaggaag tgttattggc atcctatgcg ctgccgttat ctgaattaat
    10021 tgtacgaata aagcatgagt gcgtaaatgc gcatttgtgc tttatttttt tgtttcgagg
    10081 aaagcgtagt tgtttattca tttgtcttaa taacttaagt tttgttaaat gagaacgtta
    10141 taaaatttta taatttaatt ttaataataa atgttgtata attggtgttt tttaactaaa
    10201 aaggatatca tttgatttaa aattaaaatt aaattaaatt ataattagat gggcattgtt
    10261 ttgttgattg ttgtgaaaga agacaaatat ttttgaaagg agtataagga tgtgaaaggg
    10321 ttaaaattga agcttttgca gttagtaact gtcagtttgc tatttggacc atatttccag
    10381 tcagtctatc atatgacgac cctggatgta gctgctgagt caacagaaac aaaggaggat
    10441 gaggtattct tagatgagga ctatgcaaaa attgaaggga agtatattga aaaagaagaa
    10501 acccttgaat ggcatctggc ttttgaaaaa aaggaaagtg ccaatgaagg tagaatacgt
    10561 ttagcaatcg atgcggcagc cgccgggatt ggtaccgtaa aaaacgttcg tggaacaggt
    10621 ctatcctcct atgatgaaga tggagaagac ctaaaagtcg agacgatcga tggtcaagaa
    10681 tggtatgtcg gaaaagttta ttcaaaagaa gcagaaaccg gcacattgat ttttgaaaca
    10741 gaaaagatcc ccgatgtgaa cgaagggtaa ctacctcttc aagtggtggt tgatgaaaga
    10801 atagataacc aagaattaca agaaaatgaa actgatacag aagattccgc tgaaattcaa
    10861 cgtagcgaag aaaatgagat ggaaaagctg gacgaaccaa tcgtacccag cggtcaagca
    10921 ctccagcgca ttcaacgcca gttattcaac ggtattttgg agcgtcttgg gccaacagct
    10981 acgcaagacc cctatgaata tatctatcct agcggagaag ttgaaaacga acagcatcgc
    11041 tacccgaagt ttacaacgaa tgactataca aaagtgttag gaaattggac acaaagcaat
    11101 gaacgctatc aaggagacaa aggctcgcca tatgacggag agaacttgat cgatgatagt
    11161 gattatacaa acgggggagc gaactggcga aattacgact atgctactaa taatggatca
    11221 actgcaggcg aagaagatag aaacgttcca gctcaaaccg ttcagctttg gggaaatgaa
    11281 cggaattttg aaaacagtta cttggattat aacggggcgt acatcaaaaa atgggtagaa
    11341 cctgttctgc caagcagcac agcatctgat ctacatcctg aagatgcaac aaccctttac
    11401 aatgtctatc tagatgtcat cgggggcgag aaaaaggaaa tcagtccaat cgatatcgtt
    11461 tttgttctag ataagtcagc gagtatgagt gaattaaccg ctgggaccaa tagtcaaact
    11521 aaaaatgctg cgttgattga ggcagtaaac gaaatgagta aagatctgct ctctgacccg
    11581 agtctggata ttcgaatcgg tatggtgaat ttttaccata atagtacagc tatcaataac
    11641 catgagcaaa tcagttcgga catctttcca ttgacaaatg atatcaatcg tttgacggga
    11701 agtgagaata cagcattaaa tcgtacacca atcggaggaa ctccgcttac actagggctt
    11761 aaaaatggct atgaaacatt gtataaagac aatggcggag aaaatagaaa tccagaaaaa
    11821 atcctaatcg ttgtgggaga tgggacaccg accttcagtt atgcaccgat tcagtcaagt
    11881 tatcgtacga gcactaatgg agcttggagc aactggactg tgatggaaga taagattgct
    11941 gaagataatg acgttttgtt cagaaatttt gaggagtttt ctgggaatac ttctaatgca
    12001 gggtttactc atccagttac ctatgcatct gactttaacc gtccagaaga tgaagtaaat
    12061 gtacactatc gttatggaga ggtaaaagaa ggagataata aagcgactca ctgggttggt
    12121 gatggttctt ctaataacaa tacgaatggc agtcctactt cgcaagaaaa gtcatctgcg
    12181 atcaacacag ttgcttatca tcattggctg aaaaacaagt atcaagagaa cccgccaagt
    12241 atcttctcca ttggtttagg aatcgatgga aacgttagcg ggagacaacg actagatgct
    12301 attggacgaa atgtattgaa aaatattgct gatttagaag aagatggcgt aacaccgcga
    12361 tattacaatg cgaacaacaa gaatgacatt gttacagcct tggaggatat ctcttctacc
    12421 ttcaagagaa cgatccagca agccacactt tatgatgaaa ctggttttaa tgtctcacca
    12481 tttggagctg gcagatcggc agatattcag ttttaccatc tagatccagg agaaaacggc
    12541 atgaagtacc aagagccaga aatatgggat gaagaacgtc atggacaagc cccaccagaa
    12601 gtagtggcga ctccgacaac gtatgtcgga cgggaaaacc aagcttataa attttctccg
    12661 atttctttag gtgaagggga aatggtcagg atcaagtacc aagtaaaact tgatgcggga
    12721 gctcaagacg gaaatttttg cacagtgaac aatatggcct atattcagaa cctggaggat
    12781 tatgagaatg atatcactac tggacggatg tatttcccat cttcttccat tcgttacgaa
    12841 cataaaggcc gtaatttcca agtcagcaag aagggtcaaa atggcgagcc gttggctgga
    12901 gtcgaatttg cattatatga ccaaaatcca gagagtgggg aagccgaacc aatcgaaaca
    12961 agtaaaacta ccacggacgg aatagcgatc tttgaaacga agatgccgat tttaggagat
    13021 gaaactggag cagtcagcga catattttgg atcagagaga tcgctggacc accacgttat
    13081 cagttagtag atgagacgta tcaattccaa atcaaaaaat atgcagccaa cgaagatgag
    13141 atgttaggtc actacgagct tgtagatgaa tatacatctg gttcgagagt cggcggaccg
    13201 gaaggtaaag atccgaattc ctattatgaa ggagaatatc acaagctttc tgttgtacaa
    13261 gagcctaaca tagaaggcac agagtatgat gagttgtatg tcaaactgga aatgcgaaat
    13321 gaattcaagc caatccacat gaatatcaat aaactaattg aaggcagtaa tatgccaatc
    13381 aatggcgcag agtttgaact ttatgaaaca gatggtaacg gactggaaat cggatatcca
    13441 gtagcgaaag ggctttctgg tgtgaacgaa gtagaggaag agaaagggag actcacgttc
    13501 tattcagtag atgaagacgg gaaatatgta atcgtggatg gtgagaaagt tattcatcca
    13561 atcggagagc cagattcatt tgtttataag gatagtgaag aaactaccaa ctatgcagaa
    13621 tacaaaatca ttgagagcca gaacccagaa ggtttcaaag aacctgatga aaatgacaca
    13681 tggatcttgc gactttacga taatgctata gggactatcc aactcaagca aacaggtgaa
    13741 gaaaattggc aatatctcac gtatacaaca gatgaagagg atcgtttatg ggtagcattc
    13801 actgcttaca atacgtttga ttatcgggag atccgagtta gaaaattaga ccatgaaggc
    13861 catacattag atggtgcagg tttcgatatc aggaaagtgg gggattcttc cttcccgctg
    13921 tatcgagcat acactggtaa tccgttagaa ccagaagaat cgaaacgttt gcctgggatc
    13981 ggatacttct attctcgtgc cgtaggagta gaaaagtatg tcgatttaaa ttacgaccaa
    14041 cctctgcatc tgtctattgg tgaatatgag gtctcggaat ctgtcgcacc aaatgggtat
    14101 cggctatctg atcaagtgtt ccagtttgca ctgaatgaag aaggtaagtt tatcattgaa
    14161 gatacggtca tcgaagacga aactgctccc ttgccggaag gctatgaaat catcgatggt
    14221 gtactacaag taaccttaga agatgaacta gcgccaatcg atttggaact tttgaaaatt
    14281 gacagtatga atagccgacg tcttgaaggc gcagaatttt caattgagaa acaaaatagt
    14341 gcaggagagt atgaaatcat cgagggaggc ttggcaccag atccaaacga ttcttcccta
    14401 ttcaatgctt cagacttgtt ggaaggggtc tatcggatca aggaagtgaa gtcacctgac
    14461 agctatagaa aacttccagg ttactttatt ttagaaatta gttatcgaga agaacctgac
    14521 atagaaaatg atcgggtagt tccaggaaaa gaagcgggta cactaaaagt tgagatttcg
    14581 tattatccaa acgaagaagc agagacgcct gatctacagc aagagttaga atatgaactt
    14641 ttggagacaa atcgaatcag attacagctt agtgtcggga acgatcctga aaatccgttg
    14701 cctgctacag gaggatccgg acgattattc tatatcctgc ttgctgcatt cttcatggcg
    14761 cttgcaggag cagcctatgg tttgtttgtc agacaatcta aaaaggaggg agcaaggtaa
    14821 tgaaaaagct gttgtgtttg atgcttcaat tacttgtttt actaccgctt gcagcagtcc
    14881 ttcttccaca tgcgatgtcg gcagaagaat caggggaaga tgtcgtggaa ttcgtgttgc
    14941 ataaacggat gattcgagat atcgattata acgatcaatt tgaataccat gaaaatgatg
    15001 gcttggctat ctctgaggaa gcaggggaaa cagcagatat catttcacag acggtgccac
    15061 taaatggagc gacatttgcc atttacgaca tgaccgatta ttatcatgaa aaaaaagctg
    15121 ctgaaaacat gactcctaaa gaattcgtcc agcaaatcgc tgaaacgaac agaaacaata
    15181 tccgtcagct aatcacagaa gaaaggcttg tccaaatagg gacttcggta acaactggga
    15241 aagatacggt tggcggatta ggagacggga ttgcacgtat caaagcacct aagaaaaacc
    15301 aagatcggga tgcagtttat ttgattttcg aagaatcgat tgatccagaa gttggattgg
    15361 atgttgatat cgatcagaca gctatcccaa ttgctgctat tttgccaatt tatcacccga
    15421 cagaatcaac cgaggaactg caagagattc atatctatcc taaaaatgtc ggttatctaa
    15481 gagatccata cttcttcaag tatggacgtc aaagaggaac aactgaaaaa ggaaaaccat
    15541 tagaaggtgc tgtttttgct ctatatcaga tgatagaagg ggggaaatat tatttagata
    15601 tggctcctgc taatgacttg aaaaatcaat ggatcaaacc agcagacaat gatcctttga
    15661 atgacaaaaa tgtaagcaaa ttcatttcgg ataaagaggg ccttgttacg acaggacaac
    15721 gtttcttgcc ttctggcacc tattattttg aagaattaaa aggtgtggaa ggttacgaga
    15781 tggatgaagc aagtaagaga atcgaggtca tcatccctga attttgggtg gatgcaaaag
    15841 gccaaccaca atatgtagta gttaacggac aggagatggc tgaattagaa tccgggaaag
    15901 ttccagtctc tgcctatgaa tccgcaacac cacgtgttta taacgagacc gtaaaagaaa
    15961 caacggacaa accgaaagag ccgagtggtc caagcaatcc aggtcaatca ggaaaatggt
    16021 ccgtattccc gcaaacgaat gaagcaagaa cgttgatttc tttactagga attcttttat
    16081 tgttaggtgc tgttttatta ataaaaaaag aacggggaga aaaaaatgaa taaaaaaatg
    16141 attgtcacgg gaatgagtgt actgttactc gttactgcgg gaatagggat tttcgggaac
    16201 ggaaaactag tcaaagccga agaagtcgca caaaaaccaa gtgaagtgac gattacatta
    16261 cacaaaaaag gtttctcatc tgttccggaa gaacgaccga atagcggatt ggtttctacg
    16321 gattttggtg aagaaaatat tccaggagtc gattttgatt tgtttgacgt aacggaagta
    16381 tattacgatt tgattcgaga taatccttta acaccggaac gagaagacgg tcttaattct
    16441 gccgaagcga ttgaatggat tcaaaaacgg cacacagaaa gttggttttt aaaatatcga
    16501 ctgacaagta ttgacaaaca aacaacgaat gaagctggag aagcggtatt ctctaccgtc
    16561 caagtgacgg aagaagcacc ttcttctaga gataaagtct atcttttctt agaaacttat
    16621 tcgccagctc atatttccag aatcgcgtct ccaatggttg ttatgatgcc agtgatgatg
    16681 cctgatatgg tggatggcgt gtgggatggt tctacatgga aagacacata caatacagat
    16741 gtgcatttgt atccgaaaaa cgaaatcaga gaagcagata aacagatgaa tgtagaggaa
    16801 agcgacctta gacaagtaac gattattaat gaagctggag aacaagaaac gatttcttat
    16861 attgatctag aaagaggaaa aacggcttct tacacgatca cagcacctat tccttatttc
    16921 atcgattctg tccttgaaaa cggaagcgct gttatcaaaa attacaaaat cacagatacg
    16981 cctacagttg gtctaactta ttatgatcag gaaatagaag tgcgagccgg cgagacgatt
    17041 ttaacaaaag gacaagatta tatagtagaa gtagtaagca acgggtttgt cgtgacgatt
    17101 ttgacagaag aaaatggagt agccaaagta gatactttag gtagattagc agatgcacgt
    17161 ggaggagatt taacgatcac gtataatttg aaggtttcca cagaactaga agcggatgat
    17221 ttccataaca acacagcggt tattgaaatc ggacgaaatg atgaatttga ttatgaagaa
    17281 ggagtagagc cgccagagaa agtcacaaca ggtggaagaa agtttgaaaa atatgatgca
    17341 tcaagcagtg aattgcttaa agatgcaaga ttcgaattgt ggaatgaaga ccgttctgaa
    17401 tacgcaatat tttataaagg ggagtcgcct ttagctgttt atgaatcagg agcagataga
    17461 attgagtggg caacaagcgg acaagcaaca gaatttgtcg cagatggaaa tggatacttt
    17521 gaggttcaag ggttagatta tggaacctat caaatgaaag agaccatggc gccagaaggt
    17581 tatgtacttc caacaggtga ggcagccttt actgaattta tcatctctta tggaagctat
    17641 aatgaagaaa ttcagatcgt aggagtagaa aatccagggc cagaaagagt accgaatatg
    17701 aaacgcgggt cacttcctgc tactggtggg aacgggctct tggcattctt actaatcgga
    17761 ataagtttga tgattggagc gtacagctgg taccgtaaat ctaaaatgaa gtctgaagta
    17821 taagcaacaa aaataagctg cagcaatcgg ttttataaag gttgctgcag tttttttaaa
    17881 taagaaggtg aagaaaatga aaaagaaaat aggtaataga cctcaaaccc aaatcagtct
    17941 tttattacaa ttatttatgg cagtgatgtt tcttgccgga gctgcagttt ttacatatcc
    18001 atttttagca gatgctctca gcaattattt ggatcaaaga aggatcgaga attatcaaaa
    18061 gcagcttgaa cgcgataaag aagaaaaaca tgagcaacaa ttagcagtgc atgaaaagaa
    18121 aaatcagacg cttgctcata catcagtaat cccaggaatg ggacaagtca aagatccttt
    18181 tgagcaagcg gttagaaatg tccaaaatcc aggaaaagaa tattacgaac aacatatgat
    18241 cggagccatc tatattccga aaatcaatgt tagcttgcct ttatttgatg aaacgaatga
    18301 tcttttgtta gatagaggag cgacagtcct gcaaggcacc tcttttccaa ctggaggaga
    18361 gagtacacat tccgtgatta ccgcccatag cggtattgct gagaaaaaac tctttacgga
    18421 tcttgaagaa atggagcaag atgaccgatt ctatcttgaa gtgtatggtc ggatgctcgc
    18481 ttatgaagtc gttgagaaaa ttgttgtcct tccaacaaaa acaaatacat tagcaattag
    18541 agaaaagcag gatctagtca cacttatcac ttgtacacct tacaccgtga acactcaccg
    18601 attactagtg acaggaagac gagttccctt tacagaagaa gtctcttcaa agatagaaca
    18661 gacaaaaaaa taccatcttt atcgattact tatactcctt ctaggtatat tgctagtgct
    18721 aacgcttttc ggttattggt gttatcgaaa atttaaaaga caaaaacaac gaaagaaaaa
    18781 taacagataa tagaccaatt attaagaata acataataaa tttaagctgc ttggaagaga
    18841 caaacgcgat taaatcgcat atttccagtt ttttttaacg tttgctaaaa tgacattttc
    18901 caggtaagcc aacttgtttc acaagggctt ctttgatatt gtagtagcaa gaagaaagat
    18961 ggagggattt tttatggcaa aaatcatggt agtcgaagac gaagagatca ttcgacaatt
    19021 gatcatggag gaacttgaga aatggcaatt tgaaaccttt ggaacgaccg atttcaatca
    19081 agtgttctca gactttgaaa gagaagaacc acagcttgta ttactagata ttaatcttcc
    19141 cgttttggat ggctattatt ggtgtcaaaa aatacgtgaa gtatcgaaag taccgatcat
    19201 ttttatctcc agcaggaata cgaatatgga tatgatcatg gctatgaaca tgggggcaga
    19261 tgattttgtg acaaaacatt tccaaatcga tgtactgatt gcaaaaatca atgcattact
    19321 tcgccgttct tacaactaca cagaattatc tagtgaaatg atgagccaca atggcattac
    19381 gttgaatgtt gacaacggaa gtatggaaat caacggagag atcattgatt tgagcaaaaa
    19441 tgaatatcgt ctattattta ttttgatgaa gcacaatgga aagatcttaa gtcgggaaaa
    19501 attgctgcga gcactgtggg atgacgaacg atttgtagat gacaatacat tgacagtcaa
    19561 catcaatcgg ttacgccgaa aaatcgaaca ggcaggtatc caaggatata tcgaaacaaa
    19621 agtaggacag ggatatatcg ttccctgaaa ggatgagcgg ttaagatgaa tttttttaaa
    19681 tatttgaaag atcagtggac attgatcttg ggatggctgt tttttattgc attgacgatc
    19741 tttgtgatgt ggctggcgcc aaacatcaaa gttgatttga gcacaattgc ttatttagct
    19801 ttgattgagg gcgtctttct gattttttat ctgctgggtg gttacttgag caagagaaga
    19861 tggtggggaa aattagccga catacaaaaa acttcccctt tgcaaaatta tttgagtggt
    19921 gggaggacag aagaagaaaa actggtagaa gaatatatca atcagctgat ccgtgaacac
    19981 caagaagtaa tgcaatcagc aatcagtaat caacaagacc aaaaagatta tattgactca
    20041 tgggtccatg aaatcaaagt gccactttcc gcttctaaat tgctagttcg ttcgatcgag
    20101 ttcgatattg acgatcaaaa gtatacatta ctagaaaatg aattatcaaa aatcgatgaa
    20161 tacgtagaac aagttttgta ttatgcaaga ctcgacagtt tctcgcggga ctacctcatc
    20221 caagaatatg gactaaaagg aatcattcag cctgtgatac gcagtcaggc aaattatttt
    20281 atccaaaaaa atattcgcta cgagatcgtt ggtgaggatc atactgtttt gaccgatgct
    20341 aaatggatcg cttttatctt caaccaattg cttagtaatg ccatcaaata tacgccggat
    20401 cacggaaaca tcattgtgag catagaaaag gaagcgcaag gagtcagcct ttcagttaaa
    20461 gattcaggga tcggtatccc agcggaagac ttgaaacgga tctttgataa gggatttact
    20521 gggagaaatg gtcgacttag caaaacacat tcaacaggat taggacttta tttagcaaaa
    20581 aatctggccg aaaaattagg tatccatttg acggctgaat caactgaagg aaaagggaca
    20641 acaatgactt tatttttccc aatcttaaat ttttataatg aaaaacgtta gaaaatcgtt
    20701 agaaacgaaa aaaagtgctt gtgttgttaa cgttttcgct gataagtttc ttatcatgtg
    20761 aaataaaagg agattggtta tggaaatttt aatagcatta gtccctatgt tggcatgggg
    20821 tagtattggt ttagtaagtg gtaaaattgg tggcgatgcc aatcaacaaa cactaggaat
    20881 gacgatcggt gcatttctgt tttctttagt tgtctttttc gttgttagtc ctgtaatcac
    20941 cccttggatt ttcttgatcg gatttttatc cggcttagct tggagtgttg gacaaaatgg
    21001 acagttccat ggaatgaagt acatgggtgt ttctgtaggg ctaccattgt ctactggttt
    21061 tcaattaatt ttgaatacga ttgcgggagc cgttttcttc catgaatgga cacaaacgaa
    21121 agattatgta tacggtatta ttgctttagc tcttcttgta agcggtgcgt atttgactgc
    21181 ccgacaagat gacgaaggaa aaatagatac agataataaa atgcttgatt ttggtaaggg
    21241 ttttagagca ttgatcttct ctacgattgg ttatggtgta tataccatca tcgtcaactg
    21301 ggcgaatttg gatgcgatgt caattatctt acctcaaagt atcggaatga tcttaggcgc
    21361 aagcttcttt gctttccgta aagttaaagt cgatcagttt gtgtggaaaa acatgatctg
    21421 tggattatta tggggcttag gaaacatctg catgcttcta acagtcaaat cacttggttt
    21481 agctgttggc ttctcacttt cacaaatggg gatcattatc tctactttag gtgggatttt
    21541 catccttggc gaaagaaaaa cgaaaaaaga gctcatttat gttattgttg gttgcctttt
    21601 agtcatttta ggtggtatcc ttttagggta tatgaaaaca gtttaatcgc tgttttccaa
    21661 cctaaaatgg ggaggaactt caatggattt gtttcgtaag aaagagatca atctcaatca
    21721 tacgagtgag atgaaaaaag aactaaaaac gtctgatttg atcatgcttg gaattggtgc
    21781 gatcatcggt acgggaatat ttgtggtgac aggggttgcg gccaatcaaa atgctggtcc
    21841 agcactgtca ctttcatttg tcttagctgc aatcgttgtc attttgtctg gactaagttt
    21901 cgcagaattt gcttcgcgag tgccagtgat tggtgggcca tatgcctatc tgtatgttgt
    21961 atttggtgaa tttgctgcct ggctgacagg gtggttgttg atcggggaat ttttactagc
    22021 ggtttcctct gttgcttccg gatggtctgg ctatatgcaa ggttttttga aaagtttagg
    22081 ggccgaactt ccacaagcat taactggcgg gtacaatccg gaaaatggta catacattga
    22141 cttgattgcg gtgctcgtag tgatattcgt cacttacatc gtgtcgcttg aagccaagaa
    22201 agcattacgt ttgaataatg ccatggtata tgtcaaattt ggtatcatcg cgttgtttat
    22261 cattgtagga atatttttcg ttaaacctga taactggcag ccattcatgc cttttggttt
    22321 ttccggtgtg ctagatggtg cagctcttgt gttttttgct ttcttgggat ttgatgccgt
    22381 tgcaatggca gccgaggaag tcaaaaaccc gcaaaaagat gtgccaagag gaatcattgg
    22441 ttcgatactg attgctactg tcctttacat catcgtgacg ctgatcttga caggaatcgt
    22501 tccgtatact gaactaggag tcaatgaccc agtggctttt gcaatgaggt atgtaggaca
    22561 tggaacagtg ggcgcggtga tcgctgtagg ggcaatttta acgctactga cggtaacgat
    22621 ctcgatgatg tattcacttg ctcgtttact gtttgctgtc agcaaggatg gcttacttcc
    22681 taagtttatg acagaaatcg atcaaaagca tcgtacaccg aaaaaagcga cttatgccgc
    22741 tggtgtggca gctgtattct ttgccggatt tttcccattg aatgtattag cggaactaac
    22801 gaatatcatg gcgctcgcct atttgatgct tgttagtcta ggcttactga agttgagaaa
    22861 aatgtttggc aaaccaaaag ctggagaatt caaagtgcca ttcgttccgc tattgccaat
    22921 cgtctccatt ttgacttgta ttgtattgat gcttcgtctg caaactgtga catggatcgt
    22981 gttcggtatc gtaatggtta ttggactgat catttatttt ggctatggct atggacacag
    23041 caagatgaat gagaaataga ctccaatgga gaagttagaa gaaagataag aaaaacgccc
    23101 atcaacagtt gtaataaact gttgttgggc gtttgtttta gtaacaagcg aaaggtgatc
    23161 tattgggtta aatccaacag tttttgtttc agatcttctt ccatgtgtcc aagctctact
    23221 tctgcatgac gacgtttttc ttttccttct ttttggatac gcaatgtctc ttggattgtt
    23281 tcgatcaaat cattttgagt ggtttgaagg gtctcgatat ccacgattcc acgttcattt
    23341 tctttcgctg tttcgatggc tgagattttc agcatttctg aatttttctt caataagtcg
    23401 tttgttgttt cagagacttg gcgttgagca gttactgcat ctttttgacg aagcaaggtc
    23461 aatgcgatca cgacttggtt tttccacagc ggaatggctg tagcgataga tgcttggatc
    23521 ttttcagcca aggcctgatt tgtgttttgg atcaaacgga tttgtggtgc ttgctggatc
    23581 gtgatctgtc tagctaagcg aagatcatgc gtacgtttat ccagacgatc taggaattgc
    23641 gtgtaatcat tggcaatctg aacatccatc tgatcgccag tttcttctgc tcttttcatc
    23701 gcttctggaa tgatcgttgt ttgaagttct tccattttca attctccagc agcgatataa
    23761 atattcagtg catcaaagta gtctttgttt ttctggtaaa gctgttctaa catcagatta
    23821 tcttttagca gaccgtcttt ttctttatct aatttcactg aaatcttgtc aatttgtgca
    23881 ccaatctttt gatattttgc tgtcacttca tagatcgatt gtttgacctt cccgaagaca
    23941 cgctggaaga tgttcccttc tccggcacgc agttcgtcag gattggcttc ttggagacga
    24001 tacatcagtt cagttaagga atctcctaca ggaccaatat cttgtgcttg cacatgattc
    24061 aacatcgatt gtgagaattc acttaatttt gcttgggcag ccgatccgta gctgatgaca
    24121 gactgtgcat cattagcatc gatttttgct gctagctgtt tagcttgttc ttggcgttcc
    24181 gccggtagtt tgtcaattaa acgagcagct gtctgttgct cttggagctg atcaatctcc
    24241 gcttgttgtg aagtggtcag cgtatctgtt ggagttgaaa acgggttgtt caataaatct
    24301 tcaagtgtat cgttaactgg tgtaacatct tttgtttcag tcggtttatg atccataaaa
    24361 aattcctcct tgttgaatct gttctcgatt cttacatgaa tgtctctact ttagaaaaag
    24421 tactcgaaac agccagacat catctgaatg ttccgagtct tcttttatga acaaaagagt
    24481 taatcctttt gttctggttc atatttatta tcacgcttta agctctgttt cgcaacagat
    24541 atctctacgt cgatatcatc tagatcctca gcaacgaatt tttgataatc ttgtgcgatt
    24601 aaagctgaca gctggtcgat gatctgcgcg ctttcttcta gtttttcata ggtctgtttg
    24661 tttttgatct catggtcatt gatctcatta tacttatttg tcaaatcaac tagattcggc
    24721 agatgcgtat aaagaaactg acttgcttca tgcagtcggt ttggttcttt caccaattct
    24781 ttgaataaag ctttcgctgc tttgactgga tcatggcgga gatcgattgc tttaagtttg
    24841 gctgtctgct gcatattcat ttgcaattga ttgatctcat tttttgtttg attcattgtt
    24901 tgtcgaaaaa atgaaatttc attcgctgtc ataccgcttt tttcatagtg agtttcaagt
    24961 tcgtcagtca gtgcaggaat ttcttcttgt tttccctttt tcctgcgcga accaaagagt
    25021 ccccacaaaa ctaaaactac acctatcaat aaaagtccta gaacaaaaga aaaactattg
    25081 tttcggacaa agagaaatag caagaccaat gctagaagcc acggccaatt tcgttttatc
    25141 ataataaacc ctccatgtca tttatcgttg agtattgaat tcttttcgtt attcgattac
    25201 atacttattt tagcatacgg actactagaa agagtatcgg actaaagaag gattttctat
    25261 atttagagaa aatctaaggt ttatctatgg taaactaata aggagttttt gagaaaaatt
    25321 gaaaaatgaa aaaaaagcag ttatgattac aaagaaagca ggagaagata tgctgaacaa
    25381 tgagcaattt gaagaaaaaa caatcagtcg caaagaaatc tatagcggaa aaattattga
    25441 tgttgctgta gacgaagttc gtttgccaga tggaggaact tcgaagagag aactggtatt
    25501 tcatcctggc ggtgtcggga tcattgcttt tgacgaacaa gatcgtctgc ttcttgtcaa
    25561 acaatttcga aaacctttag aaaaagtgat tttggagatt ccagctggaa aaatcgatcc
    25621 aggagaagga caaaatcccg aaatgacagc agctagagaa ttagaagaag aaactggcta
    25681 tcgtgcgaag tcgctgagcc atcttacttc gatgtatctt tcaccaggtt ttgcaaatga
    25741 agtccttcat atttatcatg cacaaggggt agagaaggtc gagaatcctt tagcacaaga
    25801 tgaagacgaa gtactggagt tgtatcatct aactcttgaa gaggcgcaac aagcgatgaa
    25861 agatcagttg atttgtgatg caaagacgat ttatgcgatt caatactggg aactattgac
    25921 caaaggaaag tagagggatc ggcttatggc caaaggaccg cttattacgc gaagcgaact
    25981 tcgcaagcga caacaagcac aagcaagcga gtcattaaaa aaacagcgaa aagcagaaac
    26041 agcttatcag caagaagaaa aaaagattgc cagcttttat cgtaaagaaa gcaaaaagaa
    26101 taaaccaatc acgaaaacaa ggatcagcga acgggaaaag acgaccaaat ggaactcttt
    26161 tttgatgaaa tctcttatta ttgttatcct aatgttatgt gtggtttttt tagcaatcgc
    26221 atttatatag aaagaaggaa ttaaaaagat gaaaatcggt attatcggcg caatggaaga
    26281 agaagtaaaa attttgagag agaacctaag cgaaccatta tcatgggaac gcgcaggcgc
    26341 tttatttatt tcaggttcat taggtaacca cgaagtgatc gtcgtacgtt ccggtatagg
    26401 aaaagtctta gcatctatta ccacaagtct gttgatccaa caatacggcg tgaacatggt
    26461 gatcaataca ggttcagctg gcggaatcgg tgaaggctta caagtaggtg acatcgttat
    26521 atcagacaaa gtagcttatt ttgatgcaga tgcaacagga tttggctaca acccaggaca
    26581 attaccagga atgcctctat attatgaagc aagcacatat ttgcgcagtg aaatggttaa
    26641 agcagcaaaa gcaacgaatt tgaacgcaaa agaaggattg atcgttacag gtgatacatt
    26701 tgtcgactcg ccagataaaa tcaaagaaat cttaacgaat ttccctgaag cattagcttg
    26761 cgaaatggaa ggagccgctg ttggacaaac agctcgtcag ttcaatatcc catttttgat
    26821 cgtacgtgca atgagcgaca cagcagatca ctcggcgaca caaagctttg acgaatttat
    26881 cgaagacgca ggcaaacgat cagcagaaat ggtgatcgaa tttgtcaaac atctcgtata
    26941 aggaggacta agatgaacgc attgatctcg attgactaca catatgattt tgttgcagag
    27001 gatggaaagc tgactactgg gacagcagga caagtaattg aaaaaaagct gactgctcat
    27061 acaaagaagt ttattgatca aaaggatttt gtcgtttttg ccattgatgc gcatgatcca
    27121 aaagattcct ttcatccgga gaaccgctta tttcctccgc ataatgtgat aggaacgggt
    27181 ggacgggaat tgtacggctc tttaaaaagt ttataccaag actatgagca agaggaaaat
    27241 gtttattgga tagacaaacg gcattattcc gcttttagtg gaacagatct agacatccgt
    27301 ttgcgagaac gccagatcac agatatttat ttgaccggcg tatgtacaga tatctgtgtg
    27361 ttgcatacag cagtcgatgc gtataatcta ggatacaaac tccatatttt caaagattcc
    27421 gtggcaagtt tcgatcccgt tggacatgaa tgggcattga gacattttga gtccacacta
    27481 ggcgcagaaa ttttataaaa aaacaaagtg atttgataaa ataaaaagca caaaataata
    27541 aaagaagagc ataaaacaaa cgactttgtt ttatgctctt ttttatacgt tattttaaaa
    27601 aaacttattc tttttacgtt aataaatctc tttattatca acaacttaag ttatattttt
    27661 tctttttata aaatacactt attataattt tgttttttat tgacagtaaa aataaattag
    27721 gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa
    27781 cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt
    27841 tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg
    27901 aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg
    27961 gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct
    28021 gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg
    28081 tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa
    28141 ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc
    28201 aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa
    28261 ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt
    28321 tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga
    28381 caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg
    28441 gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca
    28501 gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat
    28561 aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg
    28621 gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca
    28681 ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa
    28741 tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc
    28801 gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg
    28861 attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg
    28921 tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg
    28981 cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa
    29041 ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca
    29101 gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac
    29161 cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga
    29221 agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca
    29281 atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt
    29341 aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt
    29401 cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt
    29461 ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga
    29521 aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc
    29581 agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt
    29641 acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg
    29701 acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt
    29761 tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca
    29821 gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc
    29881 aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa
    29941 aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca
    30001 agttttaata acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac
    30061 taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc
    30121 cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa
    30181 cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct
    30241 ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc
    30301 catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg
    30361 gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa
    30421 tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat
    30481 ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa
    30541 ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt
    30601 gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa
    30661 ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc
    30721 acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc
    30781 cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca
    30841 gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat
    30901 ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga
    30961 gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt
    31021 tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt
    31081 gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc
    31141 tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag
    31201 gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
    31261 ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc
    31321 aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta
    31381 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa
    31441 actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa
    31501 ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt
    31561 gaagctgacc acttctggca caacctctta ccagattcgc actctttttt ctggcgctag
    31621 atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg
    31681 ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg
    31741 gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat
    31801 agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc
    31861 aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc
    31921 gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca
    31981 ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga
    32041 aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg attgttctag
    32101 actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg
    32161 ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt
    32221 ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa
    32281 agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc
    32341 ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc
    32401 atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac
    32461 catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc
    32521 aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc
    32581 ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga
    32641 tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa
    32701 atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac
    32761 tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt
    32821 gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc
    32881 ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat
    32941 tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa
    33001 gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg
    33061 aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc
    33121 atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt
    33181 attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg
    33241 agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg
    33301 tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat
    33361 caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc
    33421 agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa
    33481 tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa
    33541 aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt
    33601 gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta
    33661 gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag
    33721 cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt
    33781 tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat
    33841 caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct
    33901 agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag
    33961 gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg
    34021 gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat
    34081 caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat
    34141 tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt
    34201 ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt
    34261 gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa
    34321 cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca
    34381 gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg
    34441 ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa
    34501 gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag
    34561 aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag
    34621 tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg
    34681 taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat
    34741 tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat
    34801 ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt
    34861 attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac
    34921 tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga
    34981 tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat
    35041 gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt
    35101 ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac
    35161 attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga
    35221 tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc
    35281 acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca
    35341 tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg
    35401 tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac
    35461 atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc
    35521 gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt
    35581 gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct
    35641 atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta
    35701 tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg
    35761 gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg
    35821 caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa
    35881 ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc
    35941 tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt
    36001 caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc
    36061 atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg
    36121 aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg
    36181 atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta
    36241 gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc
    36301 catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca
    36361 aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc
    36421 ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg
    36481 gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa
    36541 ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat
    36601 ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat
    36661 gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt
    36721 acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg
    36781 atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag
    36841 tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat
    36901 gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa
    36961 aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc
    37021 atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc
    37081 aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt
    37141 gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt
    37201 atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt
    37261 caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga
    37321 tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca
    37381 actagttttg aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt
    37441 tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt
    37501 gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt
    37561 gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag
    37621 agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt
    37681 gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat
    37741 catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact
    37801 aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga
    37861 taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc
    37921 agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat
    37981 gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt
    38041 tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt
    38101 catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat
    38161 tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc
    38221 aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg
    38281 gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt
    38341 gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg
    38401 tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg
    38461 cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc
    38521 ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg
    38581 gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac
    38641 actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg
    38701 gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt
    38761 agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg
    38821 cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa
    38881 actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa
    38941 gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg
    39001 aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga
    39061 cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt
    39121 gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac
    39181 atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc
    39241 gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac
    39301 gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt
    39361 tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt
    39421 tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg
    39481 catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct
    39541 agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt
    39601 ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct
    39661 tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct
    39721 aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa
    39781 atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga
    39841 gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat
    39901 gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg
    39961 aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt
    40021 agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg
    40081 cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga
    40141 acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg
    40201 tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat
    40261 tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct
    40321 agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa
    40381 attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca
    40441 ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa
    40501 taataaagat caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat
    40561 gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct
    40621 tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa
    40681 acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga
    40741 agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg
    40801 gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac
    40861 aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga
    40921 tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc
    40981 gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg
    41041 tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt
    41101 ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa
    41161 agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga
    41221 aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat
    41281 tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc
    41341 tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca
    41401 attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga
    41461 ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat
    41521 ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt
    41581 attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt
    41641 ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg
    41701 ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc
    41761 atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca
    41821 agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc
    41881 atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta
    41941 tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat
    42001 ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg
    42061 gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag
    42121 aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat
    42181 atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca
    42241 caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc
    42301 aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca
    42361 gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat
    42421 aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta
    42481 ttgactttct tagttactga ttatgcctat caatttgcct attctttatg tacagcggca
    42541 attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga
    42601 atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt
    42661 ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc
    42721 tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt
    42781 gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag
    42841 atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa
    42901 atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga
    42961 cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga
    43021 accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct
    43081 ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct
    43141 tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta
    43201 tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa
    43261 ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac
    43321 aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac
    43381 tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt
    43441 atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg
    43501 tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac
    43561 agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg
    43621 cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt
    43681 ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa
    43741 tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc
    43801 catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt
    43861 agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa
    43921 agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac
    43981 aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta
    44041 ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc
    44101 agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac
    44161 aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc
    44221 gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg
    44281 tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca
    44341 agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa
    44401 tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact
    44461 cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg
    44521 tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg
    44581 aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt
    44641 gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga
    44701 aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa
    44761 gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga
    44821 agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa
    44881 ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc
    44941 attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac
    45001 actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag
    45061 catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt
    45121 caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata
    45181 attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc
    45241 agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct
    45301 aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc
    45361 tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct
    45421 ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa
    45481 tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag
    45541 agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc
    45601 cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac
    45661 ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag
    45721 cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg
    45781 agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa
    45841 cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc
    45901 atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat
    45961 actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact
    46021 ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta
    46081 aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc
    46141 tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa
    46201 tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga
    46261 gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta
    46321 tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg
    46381 ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa
    46441 tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg
    46501 aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa
    46561 attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga
    46621 aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga
    46681 tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca
    46741 agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag
    46801 aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc
    46861 ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac
    46921 ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc
    46981 tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag
    47041 gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt
    47101 tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc
    47161 ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga
    47221 tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc
    47281 cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg
    47341 caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc
    47401 cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc
    47461 aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc
    47521 aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg
    47581 aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg
    47641 tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg
    47701 ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga
    47761 aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc
    47821 ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct
    47881 tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg
    47941 agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta
    48001 aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa
    48061 gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga
    48121 atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat
    48181 atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga
    48241 aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa
    48301 gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca
    48361 tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc
    48421 ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa
    48481 aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa
    48541 tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag
    48601 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa
    48661 gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt
    48721 cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat
    48781 tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct
    48841 aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg gattccacca
    48901 atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat
    48961 gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata
    49021 aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg
    49081 aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa
    49141 atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct
    49201 gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca
    49261 tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa
    49321 ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca
    49381 gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca
    49441 tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct
    49501 ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc
    49561 ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca
    49621 tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta
    49681 aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat
    49741 atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt
    49801 aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt
    49861 atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga
    49921 ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt
    49981 tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga
    50041 aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt
    50101 tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca
    50161 gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga
    50221 agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac
    50281 caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac
    50341 aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga
    50401 aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca
    50461 actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact
    50521 gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat
    50581 tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg
    50641 ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa
    50701 aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca
    50761 gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca
    50821 acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa
    50881 ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa
    50941 agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc
    51001 aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct
    51061 ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct
    51121 ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca
    51181 ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga
    51241 gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg
    51301 ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat
    51361 aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga
    51421 agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc
    51481 gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc
    51541 gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa
    51601 gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca
    51661 gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    51721 aattccttct tatttcttga agctgaccac ttctgtcaca acctcttatt ttgattagaa
    51781 cttgcgaaaa taaaccggtt aaagtgtgtc tagcgcaaga gatttgctat aatagacgat
    51841 aagggtaaaa tgtaacgaga aggggttgaa aaatgtgtat caaattgtaa cagattcttg
    51901 ttgtgatctg ccataccatg tattagcaaa agaaaaagtt gattttttat caatgaatct
    51961 ccaattgaat gatcaagagt tagtagatga cctcggaaag acattcaatt atgaccgcta
    52021 catgaatcag ctgaaaaatg gtgcgatgcc tacgacatca caaatcaatg ttggccgata
    52081 tttagaattt ttccgttctt atgcggaaaa acagattcct gttttatatg tggctttttc
    52141 cagtggatta agcggctctt acagtagtgc gcttcaagcg gttgaattgc tgaaggaaga
    52201 atatgaagac cctgagatct atgtttttga tacaaaagct gcaagtattg gggaagggct
    52261 tttagtattg gaagctgtaa gattgaaaaa agaaggtaaa agcatcgaag aaaccctaga
    52321 gtggctggaa agtaattatt tgaaagtcca ttcatgggta acagtagacg atctgaaata
    52381 tctagaacga ggcggtagga tttccaaagc aagtgctact cttggcagtc tattgagtgt
    52441 aaagcctatt attacggtgg ataaagaagg aaggctgacg aatgttgaca aagttcgagg
    52501 aagaaacaag tcgatccaga caatcgtcaa tgagacggta aaaacaatcg ttgcaccact
    52561 tgatcaaatc gtttatgtgg cttacgctgg ggatctagag agcgcggaga aggcaaaacg
    52621 gcttttggaa gagcagataa agatgaaaga tgtgaagctt tatcccttag gaccaacgat
    52681 cgccagtcac acaggctatg gctgtatcgc gattttttcg atgggaatca gtcgataaaa
    52741 gcagtaactc attctgtaaa aaagcccaga aattgattgt aacaaaaaca gcgactctta
    52801 aaagatagat gagcatccta tctcttagag gtcgctgttt ttttattttt taagctaaat
    52861 aggccattat gcccataact cgttcaattg tttttgaata tctgcatttt ccaaaaattc
    52921 atcatatgtc gtatcagctc gatcgatcac acctttatcc gaaacggcaa tgatgcggtt
    52981 tgccaatgtt tggataaatt gatggtcatg tgacgcaaat aagattgagc cagtaaatgc
    53041 catcaagccg tcattcaatg ctgtgattga ttctaagtct aaatggttcg ttggatcatc
    53101 taatacaagt acgttagctt tcgaaagcat catttttgaa agcatgcaac ggactttctc
    53161 tccaccagat aaaacattca ctggtttcaa tacttcttca ccagaaaaca acattcttcc
    53221 taagaagctt cttaagaagg tgttatcatc ttcttcttta cttgcatatt gacgcaacca
    53281 gtctaaaatc gttagatcag tatcgaattc tttcgttgta tcctttggta aataagaatt
    53341 acttgttgtc acaccccaac gtactgttcc agtatctggc gtaatttcac ccatgataac
    53401 tttgaatagc gttgttgttg ttatatcatt ttctgcgacg aaagcgacct tgtcttcttt
    53461 gctcaaatta aaagatatat catctaaaat tttctttcca tcaatcgtta cacttacatt
    53521 ttctacctgc aataagtcgt ttccaatctc acgttcaggt ttgaatccaa cgaatggata
    53581 acgtctagaa gaaggttgga tatcgtctaa agtaattttt tcaagcattt ttttacgtga
    53641 agttgcttgt tttgattttg atgcattcgc actaaagcgg gcaataaatt cttggagttc
    53701 tttgatttgt tcttcttttt tggcatttgc gtttgcttga agtttagccg ctagttgact
    53761 agattccaac caaaaatcat aatttcctac ataaagtttg attttaccaa aatctagatc
    53821 cgccatatgt gtacatactt tgttcaaaaa gtgacggtca tgggatacaa cgatcacagt
    53881 attttcaaaa ttgatcaaaa attcttccag ccaattgatc gattggatat ccagaccgtt
    53941 tgttggttcg tctagcaaaa gaacatcagg tttaccaaat agtgtttgtg ctaataacac
    54001 ttttaccttt tgtccaccag ttaattcact catttgctga tggtgcaatt cttcagggat
    54061 gttcagacct tgcagtaaaa cagctgcttc tggttcagct tcccatccat taagctcagc
    54121 aaattctcct tctaattcag ctgctcgaat accatcttcg tcagtgaaat cctctttcat
    54181 gtaaatcgca tctttttctt tcagcacttc atacagacgc ttgtgtccca tgatgactgt
    54241 ttcaagtaca gtatagtctt catagtcgta atggttctgt ttcaatacgg ccatccgttc
    54301 atctggtccc atagtaacgg aacctgtgga aggctgcaga tcccctgata acactttaag
    54361 gaacgttgat ttacctgcac catttgcacc aatcaagcca tagcagtttc cgggggtaaa
    54421 tttaatattc acatcatcaa atagtttgcg gtctgaaaag tgcaaactca catcatttac
    54481 agtaatcaat ttttttcctc acttttatca ttattagttt tgacaagtct tatagaatta
    54541 tataggggaa aggtaaaaga ttcaaggcaa tcgaacattc tttgtgtttc acgtgaaaca
    54601 gaatgaaaaa aataaaaata aaagcggagc gaaaatagct actataggat ttattagttt
    54661 gcaacagcat aatttttgac gttatttata agagggagta ctataaaact gtaaaagaaa
    54721 tgaaagaagg attgaaaatg gatgaaaaaa gaaccatcga ctggggctca ttgattttgg
    54781 gaattttatt tgtacttgtc tcattgttat catttcaaga ccctgtcgga aatttagttg
    54841 caattgtagt agtctttgcg atttttgcct ttattaaagg gatatttgaa ctgtttgtac
    54901 ggaacagatt aaaagagcta acaggatata aaggaaagat gcccttaata atcgggatca
    54961 tcgatattct agttggcgtc tttttcttat tcaatattgg tgcaggagtc gcagcattgc
    55021 cttttgtttt tgcagtatgg tttattgctg attcagtttt agcgttattt actgctgact
    55081 tggcaagagg agtaagtgaa ggatattatt ggtttacgat catcgtcaat attttaggaa
    55141 ttttattagg aattttctta ttgtttaatc caatttcttc tgcgttgaca ttaagctttt
    55201 tagttggatt ctattttatg ttatttggta tcacacatat cgtctatgca tttagataag
    55261 taaaatcaaa caaataaaaa agccggcttc cgattacagt tttactgttg taattggaag
    55321 ccggcttttt tatcatggac aaatatgcac gaggtgattt ttaggtgtcg agacaaattt
    55381 atgcaaaaat gtgagaagct atatacacaa attttatctt aatactcgaa gcatagcgca
    55441 gttgtcacaa cctcttttca tataattcat tcaatactgg caaatcatgg acttcatatt
    55501 tttctttgaa tgcttggatt tcttcttcag aatattttct tggatctaaa tgcagatctt
    55561 ctactggcaa tgggcgcttg ttattgatct tcacatcgat tactacagga cgatctgatt
    55621 ttgcagctgc atcaaaggca aatcgaagtt gcgagtattc tgtgactgta aagctatttg
    55681 ccccgagcgc ttctccaact ttaccgaaat ctgcatcttc taaaaataca ccaaatttct
    55741 tttgttccgt atcttcttgt tctgcttcga tgaaaccaaa agaatcattc gaaaagacaa
    55801 cattaataat tggaagctga tatttgactt gtgtaatgat atcttgcatg accatagcaa
    55861 acccgccatc accacttaag gtaaatactt gtttttctgg gtagcttagt tgtgcggcaa
    55921 ttcctcctgg cacaccatta cccatagttg cgaaccatcc agatgtcgta tgtttttgtt
    55981 taccgttcat attcaataaa cgaatagaat ggatagtcgt gttaccgaca tctgtgacga
    56041 aaatcgcatc gtcttcagca atacgattga tttctttata gacagcctct ggacgaatcg
    56101 gttcttcctc tcgatcttca aaagatttca accaatttac ccatttttct ttattttctt
    56161 gatttgctag gtaccagcta tcagcagggc gagcatcccc taactcgaca agtctttgca
    56221 aagctgtgtt tgcatcaccg aggatcgaca aagatgtttc atggcgtcgt ccaaattttg
    56281 acgcatcgat atcgatttgg ataaattcag cagttggatt aaaaaatgca cgtccaaatg
    56341 ggaagtcgct accgacaaat aagatcagat cggcttctgc caatgcttca tttccgggtt
    56401 tagtagcgac acgtccagca aaaccaagaa agttttcata atcatcagga ataattcctt
    56461 ttgctagaac ggaagcgaca acaggaactg aaaaatgttc tgagaattct ttgatttgcg
    56521 gaaatccgtt tcgcgttcct tgaccaacat aaagaactgg tttttttgct ttttcaagat
    56581 aaggcaaagc agctagcaaa tctttttctt ctggtaatat cacacttgtt ttgtgagtgt
    56641 gagcggtgga gaaaggttgt tcgtcaattt cttcaaaacc aagatcaaca gggatagtca
    56701 cgatagccac gcctttatgc tcataggcgg ctttaatggc ttcatctact acatgcggaa
    56761 gactttgtgg tgtcatgact gtacgattgt atacgctaac atctgcgaaa ataggatttt
    56821 catttagttc ctggaaagag ttatagttca tggatgtaga agcaacttgt cctaagaacg
    56881 ctactagagg cacatggtcc atctgtgcat catacaaacc attgatcaaa tgtgtcgcac
    56941 caggtcctgc agagccgaag acagcgccga ctttccccgt taattttgca tctgcagcgg
    57001 ctgcgagtgc gccgacttct tcatgacgaa cttgaatgta gttgatttcg gttttttctt
    57061 gatatagcgc atccataatt gaattgaaag atcctccggg aatgccgtaa atatggtcga
    57121 taccccagct ttcaagaact tttaccattg cgattcctgc attgattttt gacatggtgt
    57181 ttcactcctt tagcaatgat gatacataaa ttttttgtat ctagagtata gaacttttta
    57241 tcgaaaatcc caactaattc gtcttttcac aaaaatgtag tcatgttttt gggaatatgc
    57301 tagagtagta catagataga ttcaagagag gggaatggca tggttgaatg gctattgaga
    57361 cttgaacctt ggcagcaagc acttgctggg acagctttta cttattttat gacagctctt
    57421 ggtgccgggt tggttttttt ctttaaagaa atcaaaaaag aagtgctaaa cttgatgtta
    57481 ggatttgctt ctggtgtcat gattgctgca agtttttggt ctttgttaga tccggctatt
    57541 acaaaagcag aagaaaatgg agatattgct tggctagttg taagtattgg gtttggttta
    57601 ggcggtgtgt ttttatatat ggcagataaa acactgcccc atatgcattt tggaccacaa
    57661 catgaagcag aaggattgcc tacccattta aaacggacga tcttgcttgt tttttctatt
    57721 acattgcata acattcctga aggtttagcc gtgggggtag cattcggtgc agcagcaact
    57781 gcggataatc cgacagccgc tatattagcc gctgtttcgg tggcgctagg tattggtatc
    57841 caaaacttcc cagaaggagc agctgtatca atcccgttgc gacaagaagg tctgagtcgt
    57901 aaaaaggcct ttgtttacgg acaagcttct ggaatcgttg aaccaattgc tggagtgatt
    57961 ggagcgctac tagtaacgaa agtggagctg ctgcttcctt atgcacttgc ttttgccgca
    58021 ggagcaatga tttatgtcgt ggtggaagaa ttgatccctg aagcacagca gacattgtcc
    58081 agcaaacatc attttgcagt ttttggcgtc atgagtggat ttatcattat gatgattttg
    58141 gatgtggcat taggataaat aaaaaaaata tatatgatat atcaaatagg gccgcgaact
    58201 tatatcgcga ccctattttt ttagtaagtt atatcataat gaagtgaatg gtatcataca
    58261 ttaataatag ttttttctat gtgtatttcc tttgatccag cgaacaagcc agatgataag
    58321 tgcgattggg attagaactt tgaccgcaaa ccataaaagc ccgcttagga cactgacaaa
    58381 aagactgatg acgaagctag ctgcaattag cactaaaaga accatgaaga tatttaagaa
    58441 catatttttt tgacgcatac aaaaccctcc tcttgttcat tctttactat agaaaaagta
    58501 taacagggat tgtttaaaaa cacctccaac tgaagactga tttttacttc atctacttaa
    58561 atgatcaaac aattgctttt tctctggttt atcatctaaa atagagcgga aaaggaggag
    58621 aatatcatgc ctaaaaatcg tcttgaagca tttacagatg ctgtaatcgc gattatcatg
    58681 actattttag ttttagaact tcatgaacct aacggagaca cgtgggaggc gctcgctgca
    58741 ttaggttacc gattttttat ctatattgtc agttttatca ttttagcgat ttattggaac
    58801 aatcatcatc atttatttca agttgtacat aagataaatg gacgggtctt atgggcgaac
    58861 agtttctttg tttttgcgct ttcgcttttt ccgtttgcaa cttcatggat cagtgagtac
    58921 atcaatagcc tggctccaga aatgacttat ggttttgtta ccttgaccgc taatattgcg
    58981 tattatatct tggcaaaaga acttgtcaaa gcaaataaag gccacgagaa agcattgaag
    59041 gcgatgttga ataattacaa gaaatcgtat ctatcgattg gtttgaatgt gttaggaatt
    59101 gtcttagggt atttagtagc gccagtagcc gtgctgatcg caaacatcgt gattctagct
    59161 gcttggatca tcccaagtcg taccatcgaa tcacaatatc ggtaaaatga tccgtgttta
    59221 aaatttaaga gaaaaatata gaaaaaataa ataaacgatc aaatatccga gtgatactag
    59281 tattcaaaga gggatgcaga tggatcaaaa aagaattcat gtaggactga ttactagttt
    59341 tgtcacgatg cttatttatt tggatcaagg tcatttgact tggacagtag ttggtgtcgt
    59401 aggatttttg acattattca cagcaataaa aaaataaaca tctttatgta atctgatgtt
    59461 ctatctattt aagaaaagca tgtttacagc agtatgattc tgttgtaaac gtgtttttat
    59521 ttgtggaaaa tttaattttt agaaaagtgt atcgaaaaat agatagttag tttttccaca
    59581 gtggataact tcgtaagaga taaaaataaa aaattagtca aagaagggcg tatagatttc
    59641 ttttgatagg agatatacaa cagaaatgaa taaatattaa cgtgttttca aacagtataa
    59701 aacggtgtgg tttttggaat aaaaaaataa catatctcat tttacacttt tgttatttta
    59761 tacttaaaaa ataacgacac agaaaatacc gtgtctcaga tttctctact acttctaaaa
    59821 actactgttt ttttcctttg aaatgccttc aaaaagtagg atcaggcaga atatactggc
    59881 agaatcaagg agaagggagc ctaaataagg agaaaaacca tagaaaatat aaagtaatta
    59941 cttataaaac aagcttttta tagaaaaata atatacctga gtagggtata aattaagacg
    60001 gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta agcctttaag
    60061 ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt caaactgcaa
    60121 gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt taaaccaatg
    60181 actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg tcttttttta
    60241 cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta cgatcagaaa
    60301 taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg tcgatagatg
    60361 tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat tagttatcaa
    60421 atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg ccaagaagct
    60481 tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat tgacattcaa
    60541 aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta ataccgtcgt
    60601 attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag aataagtgag
    60661 aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa agaataaata
    60721 aatccctaat aaaaattttg atacataccg catagacatc tcctccattt aaaaagtata
    60781 ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca acgtagttat
    60841 cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca aagctaagat
    60901 agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg aatgttcgtt
    60961 ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga aaaatcaaga
    61021 aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct tggttcagct
    61081 ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc gtttcaataa
    61141 aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta ttgtattatg
    61201 gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag ggatctttac
    61261 ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt ttctgatcgg
    61321 ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt tatggcttga
    61381 accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat tattccatat
    61441 ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga gcatgcagct
    61501 gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg tttttgccct
    61561 gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa ttgaaaaagc
    61621 aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg ttttctacag
    61681 cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata atccttattg
    61741 gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga tgaagcatcg
    61801 acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat tcttgtcatt
    61861 tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg gaatcgcaca
    61921 atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga tggcgttgat
    61981 gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc gttttttagg
    62041 gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg ttggtttgca
    62101 acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata tgtgccaaaa
    62161 gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa gccagattct
    62221 cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa attttatatt
    62281 tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa
    62341 tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt
    62401 agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa
    62461 tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa
    62521 ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa
    62581 aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag
    62641 caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag
    62701 ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc
    62761 caagaactat ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc
    62821 aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct
    62881 tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg
    62941 atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat
    63001 ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc
    63061 ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta
    63121 aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag
    63181 ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac
    63241 atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg
    63301 agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt
    63361 aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    63421 ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    63481 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    63541 agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta
    63601 aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt
    63661 ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt
    63721 taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat
    63781 gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca
    63841 taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa
    63901 gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc
    63961 gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact
    64021 gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga
    64081 taccattccc taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat
    64141 aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt
    64201 attgatcact ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg
    64261 gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa
    64321 gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa
    64381 tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct
    64441 ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa
    64501 taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga
    64561 taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa
    64621 attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt
    64681 aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc
    64741 aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt
    64801 tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg
    64861 actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag
    64921 aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt
    64981 gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca
    65041 atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta
    65101 acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa
    65161 attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata
    65221 tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag
    65281 ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa
    65341 gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact
    65401 ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa
    65461 agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa
    65521 gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt
    65581 gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt ttattttaac
    65641 ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt
    65701 ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga
    65761 aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat agatttaata
    65821 agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa
    65881 ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat
    65941 aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa
    66001 gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa
    66061 atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt
    66121 gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc
    66181 cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat
    66241 aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta accattacgc
    66301 ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag
    66361 aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg
    66421 gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt
    66481 atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt
    66541 ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt
    66601 ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac
    66661 tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc
    66721 agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag
    66781 tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc
    66841 atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc
    66901 aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt cagtcagctg
    66961 gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag
    67021 catacgataa tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg
    67081 ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat
    67141 atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc
    67201 atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt
    67261 tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca
    67321 tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc
    67381 aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat
    67441 ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta
    67501 gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt
    67561 cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg
    67621 ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta
    67681 tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta
    67741 cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt
    67801 tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa
    67861 gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat
    67921 tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta
    67981 acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc
    68041 aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc
    68101 taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt
    68161 gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg ttttggatgc
    68221 gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta
    68281 catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga
    68341 aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca
    68401 agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga
    68461 attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt
    68521 tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga
    68581 agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa
    68641 acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta
    68701 tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat
    68761 ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga
    68821 ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa
    68881 ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa
    68941 atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc
    69001 gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc
    69061 tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt
    69121 tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca
    69181 ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg
    69241 gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg
    69301 aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa
    69361 aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga
    69421 tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt
    69481 ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc
    69541 gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt
    69601 tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag
    69661 tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga
    69721 attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt
    69781 gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga
    69841 agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc
    69901 atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac
    69961 cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg
    70021 ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt
    70081 tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat
    70141 gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg
    70201 ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg
    70261 tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg
    70321 tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag
    70381 tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac
    70441 aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg
    70501 tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac
    70561 cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg
    70621 cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct
    70681 atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt
    70741 ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta
    70801 tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact
    70861 gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt
    70921 tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc
    70981 ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt
    71041 gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa
    71101 gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc
    71161 agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc
    71221 ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt
    71281 cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc
    71341 cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga
    71401 aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt
    71461 aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc
    71521 tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta
    71581 ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg
    71641 agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca
    71701 atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat
    71761 ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg
    71821 aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat
    71881 attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact
    71941 atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag
    72001 ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta
    72061 aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc
    72121 ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa
    72181 agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca
    72241 atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag
    72301 aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac
    72361 ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt
    72421 ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt
    72481 atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac
    72541 ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg
    72601 atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac
    72661 cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata
    72721 atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg
    72781 caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca
    72841 accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag
    72901 caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca
    72961 gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg
    73021 aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg
    73081 catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg
    73141 taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg
    73201 aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc
    73261 caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga
    73321 cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat
    73381 cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc
    73441 ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc
    73501 agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat
    73561 gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta
    73621 gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac
    73681 agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata
    73741 gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa
    73801 gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt cccactccac
    73861 tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt
    73921 tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa agtcagcggt
    73981 cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag tacgtccgtc
    74041 acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc agatatcacg
    74101 gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc gtacgatttc
    74161 tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac cagggaatgg
    74221 ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac ggacttcgtc
    74281 tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg gcagtccacc
    74341 tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga taacgtcagt
    74401 atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt ttgttgcttc
    74461 gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt ctgggtcaga
    74521 tacacctttc aacttgtcta agaagcgatc tcttgcattg actttgatga tgttcaagcc
    74581 gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg
    74641 gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac
    74701 actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg
    74761 gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca
    74821 aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc
    74881 atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc
    74941 tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct
    75001 catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac
    75061 ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt
    75121 gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat
    75181 ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat
    75241 gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc
    75301 ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta
    75361 gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac
    75421 ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc
    75481 gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc
    75541 ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt
    75601 ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg
    75661 gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca
    75721 gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct
    75781 gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga
    75841 tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta
    75901 tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc atatcataac
    75961 tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa
    76021 gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg
    76081 tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa
    76141 taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt
    76201 caaattcttt catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat
    76261 cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag
    76321 gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca
    76381 ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa
    76441 taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat ctttataata
    76501 gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt
    76561 catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga
    76621 tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt
    76681 aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga
    76741 tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta
    76801 tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat
    76861 taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt
    76921 tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt
    76981 aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata
    77041 tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc
    77101 gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa
    77161 aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga
    77221 aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt
    77281 tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt
    77341 tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa
    77401 aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt
    77461 ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag
    77521 atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag
    77581 gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt
    77641 ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga
    77701 tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt
    77761 tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga
    77821 tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa
    77881 atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt
    77941 taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg
    78001 gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga
    78061 aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga
    78121 aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag
    78181 gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg
    78241 ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc
    78301 cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc
    78361 aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga
    78421 tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa
    78481 ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt
    78541 aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt
    78601 ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat
    78661 gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat
    78721 cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt
    78781 attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc
    78841 gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa
    78901 gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag
    78961 tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact
    79021 agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg
    79081 cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga
    79141 caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc
    79201 cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac
    79261 cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat
    79321 gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga
    79381 acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca
    79441 aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt
    79501 tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga
    79561 atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc
    79621 gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa
    79681 acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct
    79741 aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca
    79801 taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga
    79861 cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc
    79921 aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac
    79981 cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt
    80041 gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca
    80101 tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg
    80161 tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac
    80221 aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat
    80281 caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt
    80341 tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat cgttattaga
    80401 tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa
    80461 aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga
    80521 aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc
    80581 aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt
    80641 atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc
    80701 aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc
    80761 cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac
    80821 attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca
    80881 gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct
    80941 atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg
    81001 gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct
    81061 tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa
    81121 ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag
    81181 gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg
    81241 attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag
    81301 gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga
    81361 aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat
    81421 cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta
    81481 ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact
    81541 ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag
    81601 acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag
    81661 tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag
    81721 ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg
    81781 ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa
    81841 acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa
    81901 aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat
    81961 cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac
    82021 tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc
    82081 ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac
    82141 aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg
    82201 acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac
    82261 acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc
    82321 ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac
    82381 gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa
    82441 aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga
    82501 acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg
    82561 cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag
    82621 ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa
    82681 gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact
    82741 gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa
    82801 aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat
    82861 gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt
    82921 taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa
    82981 attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg
    83041 attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt
    83101 ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg
    83161 atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc
    83221 gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac
    83281 ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg
    83341 cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag
    83401 ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga
    83461 cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat
    83521 atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca
    83581 gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa
    83641 cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa
    83701 gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt
    83761 gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta
    83821 ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat
    83881 gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta
    83941 ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca
    84001 catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt
    84061 aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta
    84121 ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca
    84181 agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt
    84241 caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact
    84301 tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt
    84361 agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga
    84421 agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca
    84481 tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa
    84541 tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg
    84601 attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt
    84661 aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag
    84721 tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg
    84781 tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat
    84841 tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt
    84901 atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg
    84961 tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa
    85021 cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt
    85081 tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg
    85141 actatcgaat taattgcctt gatccttact tctacactgg tttattcaac accgctaatt
    85201 ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag
    85261 ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca
    85321 cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt
    85381 tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca
    85441 gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa
    85501 ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat
    85561 attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt
    85621 ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt
    85681 tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt
    85741 tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag
    85801 tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct
    85861 gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt
    85921 tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca
    85981 gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg
    86041 aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc
    86101 gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca
    86161 ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg
    86221 attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata
    86281 cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt
    86341 taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga
    86401 cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg
    86461 gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca
    86521 aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag
    86581 ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt
    86641 ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat
    86701 atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa
    86761 tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg
    86821 attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg
    86881 acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg
    86941 tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt
    87001 tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg
    87061 atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg
    87121 aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt
    87181 ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga
    87241 aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga
    87301 actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt
    87361 tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt
    87421 tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg
    87481 tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt
    87541 tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa
    87601 gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt
    87661 gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc
    87721 atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat
    87781 caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt
    87841 tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga
    87901 aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga
    87961 aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac
    88021 tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat
    88081 taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac
    88141 tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata
    88201 ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga
    88261 ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc
    88321 cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac
    88381 gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat
    88441 tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa
    88501 aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga
    88561 tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga
    88621 aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg
    88681 aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt
    88741 taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga
    88801 cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag
    88861 aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat
    88921 aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc
    88981 tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt
    89041 taccaagagg ttgtgaa
//
LOCUS       DAZKSR010000002        84900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000002 DAZKSR010000000
VERSION     DAZKSR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.002"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..1015)
                     /locus_tag="ACG8XG_000082"
     CDS             complement(218..1015)
                     /locus_tag="ACG8XG_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0772032.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="ACG8XG_000083"
     CDS             complement(1012..2196)
                     /locus_tag="ACG8XG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0772033.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="ACG8XG_000084"
     CDS             complement(2221..3468)
                     /locus_tag="ACG8XG_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0772034.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="ACG8XG_000085"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="ACG8XG_000085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0772035.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="ACG8XG_000086"
     CDS             5448..5762
                     /locus_tag="ACG8XG_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0772036.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="ACG8XG_000087"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="ACG8XG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0772037.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /gene="tmk"
                     /locus_tag="ACG8XG_000088"
     CDS             6447..7091
                     /gene="tmk"
                     /locus_tag="ACG8XG_000088"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0772038.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="ACG8XG_000089"
     CDS             7106..7435
                     /locus_tag="ACG8XG_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0772039.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="ACG8XG_000090"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="ACG8XG_000090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0772040.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="ACG8XG_000091"
     CDS             8423..9247
                     /locus_tag="ACG8XG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0772041.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="ACG8XG_000092"
     CDS             9240..9587
                     /locus_tag="ACG8XG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0772042.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="ACG8XG_000093"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="ACG8XG_000093"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0772043.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="ACG8XG_000094"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="ACG8XG_000094"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0772044.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="ACG8XG_000095"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="ACG8XG_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0772045.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="ACG8XG_000096"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="ACG8XG_000096"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0772046.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="ACG8XG_000097"
     CDS             15244..16011
                     /locus_tag="ACG8XG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772047.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="ACG8XG_000098"
     CDS             16001..18043
                     /locus_tag="ACG8XG_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772048.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="ACG8XG_000099"
     CDS             18324..18473
                     /locus_tag="ACG8XG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0772049.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="ACG8XG_000100"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="ACG8XG_000100"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0772050.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="ACG8XG_000101"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="ACG8XG_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0772051.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="ACG8XG_000102"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="ACG8XG_000102"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0772052.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="ACG8XG_000103"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="ACG8XG_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0772053.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="ACG8XG_000104"
     CDS             22890..23972
                     /locus_tag="ACG8XG_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0772054.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="ACG8XG_000105"
     CDS             23972..24775
                     /locus_tag="ACG8XG_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0772055.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="ACG8XG_000106"
     CDS             24845..25585
                     /locus_tag="ACG8XG_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0772056.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="ACG8XG_000107"
     CDS             25596..26600
                     /locus_tag="ACG8XG_000107"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0772057.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="ACG8XG_000108"
     CDS             complement(26645..28489)
                     /locus_tag="ACG8XG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772058.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28700..29218
                     /locus_tag="ACG8XG_000109"
     CDS             28700..29218
                     /locus_tag="ACG8XG_000109"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0772059.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29346..30029
                     /locus_tag="ACG8XG_000110"
     CDS             29346..30029
                     /locus_tag="ACG8XG_000110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0772060.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30338..30411
                     /locus_tag="ACG8XG_000111"
     tRNA            30338..30411
                     /locus_tag="ACG8XG_000111"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30372..30374,aa:Arg,seq:tct)
     gene            30570..30929
                     /locus_tag="ACG8XG_000112"
     CDS             30570..30929
                     /locus_tag="ACG8XG_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0772061.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31052..32683
                     /locus_tag="ACG8XG_000113"
     CDS             31052..32683
                     /locus_tag="ACG8XG_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0772062.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32745..33386)
                     /locus_tag="ACG8XG_000114"
     CDS             complement(32745..33386)
                     /locus_tag="ACG8XG_000114"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0772063.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    33572..33648
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            33668..33976
                     /gene="rplU"
                     /locus_tag="ACG8XG_000115"
     CDS             33668..33976
                     /gene="rplU"
                     /locus_tag="ACG8XG_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0772064.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34003..34347
                     /locus_tag="ACG8XG_000116"
     CDS             34003..34347
                     /locus_tag="ACG8XG_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0772065.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34360..34653
                     /gene="rpmA"
                     /locus_tag="ACG8XG_000117"
     CDS             34360..34653
                     /gene="rpmA"
                     /locus_tag="ACG8XG_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0772066.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35087..37894
                     /locus_tag="ACG8XG_000118"
     CDS             35087..37894
                     /locus_tag="ACG8XG_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0772067.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            38085..38576
                     /locus_tag="ACG8XG_000119"
     CDS             38085..38576
                     /locus_tag="ACG8XG_000119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0772068.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38679..39668
                     /locus_tag="ACG8XG_000120"
     CDS             38679..39668
                     /locus_tag="ACG8XG_000120"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0772069.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39701..40504
                     /locus_tag="ACG8XG_000121"
     CDS             39701..40504
                     /locus_tag="ACG8XG_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0772070.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40525..41439
                     /locus_tag="ACG8XG_000122"
     CDS             40525..41439
                     /locus_tag="ACG8XG_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0772071.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41522..41896
                     /locus_tag="ACG8XG_000123"
     CDS             41522..41896
                     /locus_tag="ACG8XG_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0772072.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42035..42280
                     /locus_tag="ACG8XG_000124"
     CDS             42035..42280
                     /locus_tag="ACG8XG_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0772073.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42452..43516
                     /locus_tag="ACG8XG_000125"
     CDS             42452..43516
                     /locus_tag="ACG8XG_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0772074.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43762..44187
                     /locus_tag="ACG8XG_000126"
     CDS             43762..44187
                     /locus_tag="ACG8XG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0772075.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44180..44629
                     /gene="nusB"
                     /locus_tag="ACG8XG_000127"
     CDS             44180..44629
                     /gene="nusB"
                     /locus_tag="ACG8XG_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0772076.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44726..45580
                     /locus_tag="ACG8XG_000128"
     CDS             44726..45580
                     /locus_tag="ACG8XG_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0772077.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45581..46918
                     /gene="xseA"
                     /locus_tag="ACG8XG_000129"
     CDS             45581..46918
                     /gene="xseA"
                     /locus_tag="ACG8XG_000129"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0772078.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46923..47147
                     /locus_tag="ACG8XG_000130"
     CDS             46923..47147
                     /locus_tag="ACG8XG_000130"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0772079.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47149..48039
                     /locus_tag="ACG8XG_000131"
     CDS             47149..48039
                     /locus_tag="ACG8XG_000131"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0772080.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48048..48866
                     /locus_tag="ACG8XG_000132"
     CDS             48048..48866
                     /locus_tag="ACG8XG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0772081.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48973..49422
                     /locus_tag="ACG8XG_000133"
     CDS             48973..49422
                     /locus_tag="ACG8XG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0772082.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49440..51122
                     /gene="recN"
                     /locus_tag="ACG8XG_000134"
     CDS             49440..51122
                     /gene="recN"
                     /locus_tag="ACG8XG_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0772083.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51177..51293)
                     /locus_tag="ACG8XG_000135"
     CDS             complement(51177..51293)
                     /locus_tag="ACG8XG_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0772084.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51366..52322)
                     /locus_tag="ACG8XG_000136"
     CDS             complement(51366..52322)
                     /locus_tag="ACG8XG_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0772085.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52493..52861
                     /locus_tag="ACG8XG_000137"
     CDS             52493..52861
                     /locus_tag="ACG8XG_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0772086.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53031..53462
                     /gene="mraZ"
                     /locus_tag="ACG8XG_000138"
     CDS             53031..53462
                     /gene="mraZ"
                     /locus_tag="ACG8XG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0772087.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53483..54442
                     /gene="rsmH"
                     /locus_tag="ACG8XG_000139"
     CDS             53483..54442
                     /gene="rsmH"
                     /locus_tag="ACG8XG_000139"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0772088.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54447..54848
                     /gene="ftsL"
                     /locus_tag="ACG8XG_000140"
     CDS             54447..54848
                     /gene="ftsL"
                     /locus_tag="ACG8XG_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0772089.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54849..57041
                     /locus_tag="ACG8XG_000141"
     CDS             54849..57041
                     /locus_tag="ACG8XG_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0772090.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57067..58029
                     /gene="mraY"
                     /locus_tag="ACG8XG_000142"
     CDS             57067..58029
                     /gene="mraY"
                     /locus_tag="ACG8XG_000142"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0772091.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58043..59404
                     /gene="murD"
                     /locus_tag="ACG8XG_000143"
     CDS             58043..59404
                     /gene="murD"
                     /locus_tag="ACG8XG_000143"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0772092.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59430..60518
                     /gene="murG"
                     /locus_tag="ACG8XG_000144"
     CDS             59430..60518
                     /gene="murG"
                     /locus_tag="ACG8XG_000144"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0772093.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60533..61753
                     /locus_tag="ACG8XG_000145"
     CDS             60533..61753
                     /locus_tag="ACG8XG_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0772094.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61909..63234
                     /gene="ftsA"
                     /locus_tag="ACG8XG_000146"
     CDS             61909..63234
                     /gene="ftsA"
                     /locus_tag="ACG8XG_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0772095.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63257..64498
                     /gene="ftsZ"
                     /locus_tag="ACG8XG_000147"
     CDS             63257..64498
                     /gene="ftsZ"
                     /locus_tag="ACG8XG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0772096.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64515..65192
                     /locus_tag="ACG8XG_000148"
     CDS             64515..65192
                     /locus_tag="ACG8XG_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0772097.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65206..65805
                     /locus_tag="ACG8XG_000149"
     CDS             65206..65805
                     /locus_tag="ACG8XG_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0772098.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65886..66107
                     /locus_tag="ACG8XG_000150"
     CDS             65886..66107
                     /locus_tag="ACG8XG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0772099.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66156..66938
                     /locus_tag="ACG8XG_000151"
     CDS             66156..66938
                     /locus_tag="ACG8XG_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0772100.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66959..67666
                     /locus_tag="ACG8XG_000152"
     CDS             66959..67666
                     /locus_tag="ACG8XG_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0772101.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67928..70726
                     /gene="ileS"
                     /locus_tag="ACG8XG_000153"
     CDS             67928..70726
                     /gene="ileS"
                     /locus_tag="ACG8XG_000153"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0772102.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70775..72301)
                     /gene="zwf"
                     /locus_tag="ACG8XG_000154"
     CDS             complement(70775..72301)
                     /gene="zwf"
                     /locus_tag="ACG8XG_000154"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0772103.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72316..72963)
                     /locus_tag="ACG8XG_000155"
     CDS             complement(72316..72963)
                     /locus_tag="ACG8XG_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0772104.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73147..73476)
                     /locus_tag="ACG8XG_000156"
     CDS             complement(73147..73476)
                     /locus_tag="ACG8XG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0772105.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73653..74381
                     /gene="sfsA"
                     /locus_tag="ACG8XG_000157"
     CDS             73653..74381
                     /gene="sfsA"
                     /locus_tag="ACG8XG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0772106.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74397..75410
                     /locus_tag="ACG8XG_000158"
     CDS             74397..75410
                     /locus_tag="ACG8XG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0772107.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75410..76687
                     /locus_tag="ACG8XG_000159"
     CDS             75410..76687
                     /locus_tag="ACG8XG_000159"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0772108.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76750..79452
                     /locus_tag="ACG8XG_000160"
     CDS             76750..79452
                     /locus_tag="ACG8XG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0772109.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79604..79921
                     /locus_tag="ACG8XG_000161"
     CDS             79604..79921
                     /locus_tag="ACG8XG_000161"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0772110.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79951..80271
                     /locus_tag="ACG8XG_000162"
     CDS             79951..80271
                     /locus_tag="ACG8XG_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0772111.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80379..81839
                     /locus_tag="ACG8XG_000163"
     CDS             80379..81839
                     /locus_tag="ACG8XG_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0772112.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81907..82128
                     /locus_tag="ACG8XG_000164"
     CDS             81907..82128
                     /locus_tag="ACG8XG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772113.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82159..82341
                     /locus_tag="ACG8XG_000165"
     CDS             82159..82341
                     /locus_tag="ACG8XG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0772114.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82341..82754
                     /locus_tag="ACG8XG_000166"
     CDS             82341..82754
                     /locus_tag="ACG8XG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0772115.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82877..84058
                     /locus_tag="ACG8XG_000167"
     CDS             82877..84058
                     /locus_tag="ACG8XG_000167"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772116.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(84129..84293)
                     /locus_tag="ACG8XG_000168"
     CDS             complement(84129..84293)
                     /locus_tag="ACG8XG_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772117.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            84447..84520
                     /locus_tag="ACG8XG_000169"
     tRNA            84447..84520
                     /locus_tag="ACG8XG_000169"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84481..84483,aa:Arg,seq:cct)
     gene            complement(84589..>84900)
                     /locus_tag="ACG8XG_000170"
     CDS             complement(84589..>84900)
                     /locus_tag="ACG8XG_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0772118.1"
                     /translation="NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRH
                     THASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKND
                     N"
BASE COUNT        27643 a        14547 c        17996 g        24714 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg aatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    28681 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    28741 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    28801 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    28861 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    28921 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    28981 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    29041 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    29101 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    29161 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    29221 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    29281 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    29341 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    29401 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    29461 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    29521 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    29581 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    29641 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    29701 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    29761 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    29821 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    29881 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    29941 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    30001 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    30061 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    30121 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    30181 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    30241 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    30301 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    30361 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    30421 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    30481 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    30541 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    30601 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    30661 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    30721 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    30781 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    30841 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    30901 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    30961 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    31021 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    31081 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    31141 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    31201 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    31261 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    31321 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    31381 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    31441 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    31501 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    31561 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    31621 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    31681 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    31741 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    31801 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    31861 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    31921 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    31981 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    32041 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    32101 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    32161 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    32221 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    32281 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    32341 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    32401 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    32461 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    32521 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    32581 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    32641 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    32701 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    32761 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    32821 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    32881 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    32941 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    33001 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    33061 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    33121 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    33181 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    33241 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    33301 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    33361 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    33421 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    33481 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    33541 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    33601 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    33661 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    33721 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    33781 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    33841 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    33901 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    33961 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    34021 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    34081 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    34141 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    34201 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    34261 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    34321 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    34381 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    34441 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    34501 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    34561 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    34621 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    34681 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    34741 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    34801 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    34861 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    34921 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    34981 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    35041 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    35101 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    35161 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    35221 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    35281 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    35341 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    35401 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    35461 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    35521 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    35581 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    35641 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    35701 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    35761 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    35821 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    35881 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    35941 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    36001 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    36061 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    36121 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    36181 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    36241 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    36301 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    36361 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    36421 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    36481 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    36541 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    36601 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    36661 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    36721 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    36781 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    36841 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    36901 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    36961 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    37021 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    37081 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    37141 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    37201 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    37261 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    37321 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    37381 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    37441 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    37501 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    37561 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    37621 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    37681 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    37741 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    37801 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    37861 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    37921 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    37981 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    38041 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    38101 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    38161 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    38221 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    38281 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    38341 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    38401 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    38461 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    38521 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    38581 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    38641 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    38701 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    38761 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    38821 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    38881 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    38941 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    39001 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    39061 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    39121 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    39181 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    39241 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    39301 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    39361 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    39421 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    39481 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    39541 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    39601 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    39661 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    39721 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    39781 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    39841 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    39901 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    39961 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    40021 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    40081 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    40141 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    40201 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    40261 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    40321 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    40381 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    40441 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    40501 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    40561 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    40621 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    40681 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    40741 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    40801 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    40861 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    40921 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    40981 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    41041 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    41101 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    41161 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    41221 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    41281 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    41341 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    41401 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    41461 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    41521 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    41581 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    41641 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    41701 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    41761 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    41821 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    41881 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    41941 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    42001 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    42061 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    42121 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    42181 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    42241 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    42301 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    42361 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    42421 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    42481 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    42541 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    42601 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    42661 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    42721 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    42781 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    42841 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    42901 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    42961 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    43021 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    43081 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    43141 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    43201 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    43261 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    43321 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    43381 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    43441 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    43501 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    43561 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    43621 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    43681 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    43741 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    43801 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    43861 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    43921 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    43981 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    44041 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    44101 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    44161 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    44221 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    44281 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    44341 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    44401 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    44461 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    44521 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    44581 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    44641 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    44701 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    44761 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    44821 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    44881 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    44941 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    45001 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    45061 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    45121 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    45181 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    45241 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    45301 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    45361 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    45421 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    45481 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    45541 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    45601 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    45661 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    45721 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    45781 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    45841 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    45901 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    45961 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    46021 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    46081 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    46141 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    46201 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    46261 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    46321 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    46381 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    46441 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    46501 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    46561 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    46621 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    46681 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    46741 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    46801 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    46861 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    46921 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    46981 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    47041 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    47101 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    47161 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    47221 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    47281 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    47341 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    47401 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    47461 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    47521 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    47581 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    47641 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    47701 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    47761 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    47821 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    47881 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    47941 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    48001 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    48061 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    48121 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    48181 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    48241 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    48301 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    48361 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    48421 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    48481 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    48541 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    48601 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    48661 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    48721 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    48781 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    48841 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    48901 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    48961 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    49021 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    49081 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    49141 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    49201 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    49261 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    49321 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    49381 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    49441 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    49501 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    49561 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    49621 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    49681 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    49741 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    49801 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    49861 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    49921 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    49981 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    50041 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    50101 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    50161 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    50221 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    50281 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    50341 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    50401 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    50461 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    50521 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    50581 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    50641 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    50701 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    50761 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    50821 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    50881 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    50941 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    51001 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    51061 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    51121 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    51181 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    51241 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    51301 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    51361 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    51421 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    51481 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    51541 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    51601 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    51661 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    51721 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    51781 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    51841 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    51901 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    51961 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    52021 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    52081 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    52141 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    52201 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    52261 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    52321 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    52381 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    52441 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    52501 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    52561 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    52621 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    52681 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    52741 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    52801 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    52861 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    52921 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    52981 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    53041 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    53101 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    53161 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    53221 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    53281 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    53341 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    53401 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    53461 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    53521 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    53581 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    53641 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    53701 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    53761 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    53821 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    53881 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    53941 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    54001 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    54061 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    54121 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    54181 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    54241 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    54301 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    54361 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    54421 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    54481 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    54541 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    54601 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    54661 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    54721 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    54781 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    54841 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    54901 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    54961 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    55021 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    55081 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    55141 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    55201 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    55261 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    55321 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    55381 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    55441 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    55501 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    55561 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    55621 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    55681 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    55741 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    55801 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    55861 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    55921 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    55981 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    56041 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    56101 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    56161 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    56221 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    56281 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    56341 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    56401 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    56461 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    56521 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    56581 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    56641 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    56701 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    56761 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    56821 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    56881 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    56941 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    57001 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    57061 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    57121 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    57181 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    57241 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    57301 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    57361 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    57421 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    57481 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    57541 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    57601 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    57661 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    57721 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    57781 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    57841 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    57901 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    57961 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    58021 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    58081 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    58141 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    58201 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    58261 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    58321 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    58381 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    58441 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    58501 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    58561 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    58621 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    58681 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    58741 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    58801 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    58861 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    58921 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    58981 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    59041 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    59101 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    59161 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    59221 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    59281 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    59341 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    59401 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    59461 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    59521 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    59581 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    59641 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    59701 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    59761 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    59821 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    59881 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    59941 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    60001 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    60061 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    60121 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    60181 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    60241 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    60301 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    60361 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    60421 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    60481 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    60541 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    60601 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    60661 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    60721 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    60781 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    60841 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    60901 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    60961 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    61021 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    61081 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    61141 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    61201 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    61261 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    61321 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    61381 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    61441 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    61501 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    61561 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    61621 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    61681 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    61741 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    61801 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    61861 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    61921 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    61981 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    62041 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    62101 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    62161 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    62221 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    62281 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    62341 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    62401 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    62461 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    62521 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    62581 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    62641 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    62701 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    62761 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    62821 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    62881 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    62941 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    63001 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    63061 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    63121 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    63181 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    63241 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    63301 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    63361 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    63421 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    63481 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    63541 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    63601 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    63661 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    63721 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    63781 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    63841 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    63901 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    63961 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    64021 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    64081 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    64141 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    64201 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    64261 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    64321 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    64381 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    64441 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    64501 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    64561 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    64621 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    64681 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    64741 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    64801 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    64861 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    64921 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    64981 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    65041 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    65101 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    65161 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    65221 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    65281 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    65341 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    65401 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    65461 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    65521 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    65581 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    65641 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    65701 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    65761 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    65821 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    65881 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    65941 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    66001 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    66061 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    66121 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    66181 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    66241 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    66301 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    66361 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    66421 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    66481 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    66541 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    66601 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    66661 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    66721 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    66781 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    66841 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    66901 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    66961 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    67021 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    67081 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    67141 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    67201 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    67261 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    67321 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    67381 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    67441 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    67501 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    67561 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    67621 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    67681 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    67741 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    67801 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    67861 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    67921 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    67981 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    68041 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    68101 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    68161 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    68221 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    68281 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    68341 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    68401 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    68461 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    68521 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    68581 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    68641 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    68701 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    68761 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    68821 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    68881 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    68941 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    69001 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    69061 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    69121 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    69181 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    69241 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    69301 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    69361 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    69421 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    69481 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    69541 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    69601 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    69661 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    69721 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    69781 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    69841 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    69901 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    69961 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    70021 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    70081 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    70141 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    70201 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    70261 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    70321 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    70381 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    70441 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    70501 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    70561 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    70621 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    70681 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    70741 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    70801 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    70861 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    70921 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    70981 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    71041 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    71101 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    71161 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    71221 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    71281 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    71341 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    71401 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    71461 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    71521 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    71581 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    71641 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    71701 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    71761 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    71821 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    71881 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    71941 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    72001 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    72061 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    72121 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    72181 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    72241 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    72301 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    72361 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    72421 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    72481 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    72541 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    72601 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    72661 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    72721 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    72781 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    72841 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    72901 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    72961 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    73021 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    73081 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    73141 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    73201 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    73261 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    73321 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    73381 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    73441 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    73501 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    73561 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    73621 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    73681 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    73741 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    73801 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    73861 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    73921 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    73981 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    74041 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    74101 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    74161 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    74221 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    74281 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    74341 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    74401 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    74461 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    74521 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    74581 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    74641 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    74701 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    74761 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    74821 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    74881 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    74941 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    75001 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    75061 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    75121 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    75181 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    75241 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    75301 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    75361 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    75421 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    75481 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    75541 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    75601 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    75661 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    75721 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    75781 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    75841 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    75901 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    75961 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    76021 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    76081 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    76141 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    76201 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    76261 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    76321 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    76381 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    76441 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    76501 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    76561 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    76621 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    76681 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    76741 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    76801 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    76861 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    76921 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    76981 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    77041 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    77101 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    77161 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    77221 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    77281 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    77341 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    77401 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    77461 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    77521 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    77581 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    77641 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    77701 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    77761 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    77821 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    77881 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    77941 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    78001 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    78061 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    78121 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    78181 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    78241 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    78301 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    78361 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    78421 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    78481 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    78541 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    78601 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    78661 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    78721 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    78781 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    78841 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    78901 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    78961 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    79021 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    79081 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    79141 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    79201 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    79261 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    79321 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    79381 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    79441 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    79501 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    79561 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    79621 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    79681 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    79741 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    79801 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    79861 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    79921 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    79981 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    80041 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    80101 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    80161 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    80221 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    80281 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    80341 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    80401 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    80461 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    80521 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    80581 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    80641 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    80701 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    80761 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    80821 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    80881 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    80941 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    81001 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    81061 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    81121 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    81181 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    81241 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    81301 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    81361 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    81421 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    81481 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    81541 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    81601 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    81661 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    81721 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    81781 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    81841 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    81901 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    81961 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    82021 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    82081 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    82141 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    82201 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    82261 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    82321 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    82381 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    82441 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    82501 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    82561 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    82621 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    82681 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    82741 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    82801 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    82861 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    82921 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    82981 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    83041 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    83101 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    83161 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    83221 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    83281 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    83341 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    83401 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    83461 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    83521 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
    83581 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
    83641 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
    83701 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
    83761 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
    83821 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
    83881 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
    83941 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
    84001 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
    84061 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
    84121 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
    84181 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
    84241 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
    84301 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
    84361 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
    84421 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
    84481 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
    84541 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
    84601 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
    84661 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
    84721 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
    84781 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
    84841 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
//
LOCUS       DAZKSR010000003        68226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000003 DAZKSR010000000
VERSION     DAZKSR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.003"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..909
                     /locus_tag="ACG8XG_000171"
     CDS             28..909
                     /locus_tag="ACG8XG_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0772119.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="ACG8XG_000172"
     CDS             922..2532
                     /locus_tag="ACG8XG_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772120.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="ACG8XG_000173"
     CDS             complement(2609..3268)
                     /locus_tag="ACG8XG_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0772121.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="ACG8XG_000174"
     CDS             complement(3551..4216)
                     /locus_tag="ACG8XG_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0772122.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8XG_000175"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8XG_000175"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0772123.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8XG_000176"
     CDS             complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8XG_000176"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0772124.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8XG_000177"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8XG_000177"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0772125.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="ACG8XG_000178"
     CDS             6530..6850
                     /locus_tag="ACG8XG_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0772126.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7819)
                     /locus_tag="ACG8XG_000179"
     CDS             complement(6962..7819)
                     /locus_tag="ACG8XG_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0772127.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7927..9231)
                     /locus_tag="ACG8XG_000180"
     CDS             complement(7927..9231)
                     /locus_tag="ACG8XG_000180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0772128.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9228..9737)
                     /locus_tag="ACG8XG_000181"
     CDS             complement(9228..9737)
                     /locus_tag="ACG8XG_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772129.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9912..12047)
                     /locus_tag="ACG8XG_000182"
     CDS             complement(9912..12047)
                     /locus_tag="ACG8XG_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0772130.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12130..13785)
                     /locus_tag="ACG8XG_000183"
     CDS             complement(12130..13785)
                     /locus_tag="ACG8XG_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0772131.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13787..14101)
                     /locus_tag="ACG8XG_000184"
     CDS             complement(13787..14101)
                     /locus_tag="ACG8XG_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0772132.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14271..14531
                     /locus_tag="ACG8XG_000185"
     CDS             14271..14531
                     /locus_tag="ACG8XG_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0772133.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14545..14859
                     /locus_tag="ACG8XG_000186"
     CDS             14545..14859
                     /locus_tag="ACG8XG_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0772134.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15589..16143)
                     /locus_tag="ACG8XG_000187"
     CDS             complement(15589..16143)
                     /locus_tag="ACG8XG_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0772135.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16269..18198)
                     /locus_tag="ACG8XG_000188"
                     /pseudo
     CDS             complement(16269..18198)
                     /locus_tag="ACG8XG_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18504..19220)
                     /locus_tag="ACG8XG_000189"
     CDS             complement(18504..19220)
                     /locus_tag="ACG8XG_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0772136.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="ACG8XG_000190"
     CDS             complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="ACG8XG_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0772137.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="ACG8XG_000191"
     CDS             complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="ACG8XG_000191"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0772138.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22690..24150)
                     /locus_tag="ACG8XG_000192"
     CDS             complement(22690..24150)
                     /locus_tag="ACG8XG_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772139.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24140..25435)
                     /locus_tag="ACG8XG_000193"
     CDS             complement(24140..25435)
                     /locus_tag="ACG8XG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0772140.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25767..26444)
                     /locus_tag="ACG8XG_000194"
     CDS             complement(25767..26444)
                     /locus_tag="ACG8XG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0772141.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26474..27565)
                     /locus_tag="ACG8XG_000195"
     CDS             complement(26474..27565)
                     /locus_tag="ACG8XG_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0772142.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27666..28100)
                     /locus_tag="ACG8XG_000196"
     CDS             complement(27666..28100)
                     /locus_tag="ACG8XG_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0772143.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28684..30141)
                     /locus_tag="ACG8XG_000197"
     CDS             complement(28684..30141)
                     /locus_tag="ACG8XG_000197"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0772144.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30138..32033)
                     /locus_tag="ACG8XG_000198"
     CDS             complement(30138..32033)
                     /locus_tag="ACG8XG_000198"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0772145.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32091..33866)
                     /locus_tag="ACG8XG_000199"
     CDS             complement(32091..33866)
                     /locus_tag="ACG8XG_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0772146.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33916..35412)
                     /locus_tag="ACG8XG_000200"
     CDS             complement(33916..35412)
                     /locus_tag="ACG8XG_000200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0772147.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35606..37228)
                     /locus_tag="ACG8XG_000201"
     CDS             complement(35606..37228)
                     /locus_tag="ACG8XG_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0772148.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37556..38869)
                     /locus_tag="ACG8XG_000202"
     CDS             complement(37556..38869)
                     /locus_tag="ACG8XG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0772149.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39114..39338)
                     /locus_tag="ACG8XG_000203"
     CDS             complement(39114..39338)
                     /locus_tag="ACG8XG_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772150.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39358..39780)
                     /locus_tag="ACG8XG_000204"
     CDS             complement(39358..39780)
                     /locus_tag="ACG8XG_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0772151.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39922..40632)
                     /locus_tag="ACG8XG_000205"
     CDS             complement(39922..40632)
                     /locus_tag="ACG8XG_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0772152.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40650..41147)
                     /locus_tag="ACG8XG_000206"
     CDS             complement(40650..41147)
                     /locus_tag="ACG8XG_000206"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772153.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41184..41636)
                     /locus_tag="ACG8XG_000207"
     CDS             complement(41184..41636)
                     /locus_tag="ACG8XG_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0772154.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41771..42376)
                     /locus_tag="ACG8XG_000208"
     CDS             complement(41771..42376)
                     /locus_tag="ACG8XG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0772155.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42826..43446)
                     /locus_tag="ACG8XG_000209"
     CDS             complement(42826..43446)
                     /locus_tag="ACG8XG_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0772156.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43448..44317)
                     /locus_tag="ACG8XG_000210"
     CDS             complement(43448..44317)
                     /locus_tag="ACG8XG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0772157.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44340..44747)
                     /locus_tag="ACG8XG_000211"
     CDS             complement(44340..44747)
                     /locus_tag="ACG8XG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0772158.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44766..46055)
                     /locus_tag="ACG8XG_000212"
     CDS             complement(44766..46055)
                     /locus_tag="ACG8XG_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0772159.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46082..46384)
                     /locus_tag="ACG8XG_000213"
     CDS             complement(46082..46384)
                     /locus_tag="ACG8XG_000213"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0772160.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46409..48502)
                     /locus_tag="ACG8XG_000214"
     CDS             complement(46409..48502)
                     /locus_tag="ACG8XG_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0772161.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(48991..49755)
                     /locus_tag="ACG8XG_000215"
     CDS             complement(48991..49755)
                     /locus_tag="ACG8XG_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0772162.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49866..50735)
                     /gene="mdcB"
                     /locus_tag="ACG8XG_000216"
     CDS             complement(49866..50735)
                     /gene="mdcB"
                     /locus_tag="ACG8XG_000216"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0772163.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50760..51836)
                     /locus_tag="ACG8XG_000217"
     CDS             complement(50760..51836)
                     /locus_tag="ACG8XG_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0772164.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51860..52453)
                     /locus_tag="ACG8XG_000218"
     CDS             complement(51860..52453)
                     /locus_tag="ACG8XG_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0772165.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52457..54133)
                     /gene="mdcD"
                     /locus_tag="ACG8XG_000219"
     CDS             complement(52457..54133)
                     /gene="mdcD"
                     /locus_tag="ACG8XG_000219"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0772166.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54123..54422)
                     /locus_tag="ACG8XG_000220"
     CDS             complement(54123..54422)
                     /locus_tag="ACG8XG_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0772167.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54524..56170)
                     /gene="mdcA"
                     /locus_tag="ACG8XG_000221"
     CDS             complement(54524..56170)
                     /gene="mdcA"
                     /locus_tag="ACG8XG_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0772168.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56177..57088)
                     /locus_tag="ACG8XG_000222"
     CDS             complement(56177..57088)
                     /locus_tag="ACG8XG_000222"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0772169.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57208..58065
                     /locus_tag="ACG8XG_000223"
     CDS             57208..58065
                     /locus_tag="ACG8XG_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0772170.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58252..58614)
                     /locus_tag="ACG8XG_000224"
     CDS             complement(58252..58614)
                     /locus_tag="ACG8XG_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0772171.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58803..58985)
                     /locus_tag="ACG8XG_000225"
     CDS             complement(58803..58985)
                     /locus_tag="ACG8XG_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772172.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            58960..60399
                     /locus_tag="ACG8XG_000226"
     CDS             58960..60399
                     /locus_tag="ACG8XG_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772173.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60406..62958)
                     /locus_tag="ACG8XG_000227"
     CDS             complement(60406..62958)
                     /locus_tag="ACG8XG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0772174.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63072..64403)
                     /locus_tag="ACG8XG_000228"
     CDS             complement(63072..64403)
                     /locus_tag="ACG8XG_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0772175.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64369..65847)
                     /locus_tag="ACG8XG_000229"
     CDS             complement(64369..65847)
                     /locus_tag="ACG8XG_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0772176.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66152..67987)
                     /gene="tdc"
                     /locus_tag="ACG8XG_000230"
     CDS             complement(66152..67987)
                     /gene="tdc"
                     /locus_tag="ACG8XG_000230"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0772177.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19406 a        14562 c        11674 g        22584 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9181 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9241 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9301 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9361 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9421 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9481 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9541 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9601 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9661 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9721 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9781 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9841 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9901 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
     9961 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10021 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10081 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10141 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10201 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10261 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10321 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10381 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10441 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10501 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10561 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10621 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10681 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10741 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10801 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10861 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10921 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    10981 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11041 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11101 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11161 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11221 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11281 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11341 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11401 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11461 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11521 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11581 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11641 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11701 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11761 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11821 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11881 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    11941 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12001 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12061 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12121 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12181 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12241 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12301 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12361 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12421 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12481 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12541 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12601 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12661 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12721 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12781 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12841 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12901 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    12961 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13021 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13081 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13141 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13201 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13261 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13321 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13381 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13441 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13501 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13561 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13621 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13681 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13741 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13801 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13861 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13921 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    13981 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14041 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14101 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14161 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14221 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14281 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14341 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14401 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14461 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14521 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14581 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14641 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14701 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14761 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14821 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14881 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    14941 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15001 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15061 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15121 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15181 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15241 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15301 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15361 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15421 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15481 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15541 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15601 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15661 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15721 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15781 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15841 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15901 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    15961 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16021 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16081 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16141 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16201 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16261 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16321 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16381 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16441 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16501 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16561 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16621 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16681 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16741 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16801 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16861 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16921 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    16981 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17041 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17101 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17161 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17221 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17281 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17341 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17401 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17461 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17521 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17581 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17641 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17701 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17761 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17821 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17881 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    17941 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18001 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18061 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18121 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18181 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18241 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18301 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18361 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18421 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18481 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18541 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18601 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18661 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18721 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18781 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18841 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18901 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    18961 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19021 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19081 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19141 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19201 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19261 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19321 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19381 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19441 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19501 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19561 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19621 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19681 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19741 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19801 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19861 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19921 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    19981 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20041 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20101 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20161 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20221 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20281 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20341 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20401 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20461 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20521 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20581 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20641 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20701 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20761 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20821 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20881 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    20941 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21001 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21061 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21121 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21181 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21241 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21301 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21361 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21421 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21481 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21541 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21601 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21661 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21721 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21781 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21841 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21901 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    21961 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22021 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22081 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22141 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22201 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22261 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22321 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22381 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22441 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22501 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22561 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22621 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22681 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22741 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22801 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22861 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22921 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    22981 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23041 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23101 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23161 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23221 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23281 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23341 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23401 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23461 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23521 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23581 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23641 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23701 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23761 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23821 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23881 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    23941 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24001 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24061 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24121 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24181 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24241 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24301 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24361 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24421 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24481 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24541 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24601 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24661 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24721 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24781 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24841 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24901 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    24961 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25021 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25081 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25141 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25201 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25261 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25321 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25381 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25441 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25501 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25561 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25621 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25681 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25741 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25801 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25861 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25921 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    25981 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26041 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26101 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26161 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26221 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26281 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26341 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26401 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26461 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26521 aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26581 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26641 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26701 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26761 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26821 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26881 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    26941 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27001 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27061 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27121 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27181 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27241 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27301 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27361 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27421 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27481 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27541 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27601 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27661 aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta
    27721 cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg
    27781 acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg
    27841 ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat
    27901 ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat
    27961 atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct
    28021 aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct
    28081 ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt
    28141 tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag
    28201 aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt
    28261 gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat
    28321 ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg
    28381 tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa
    28441 aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac
    28501 cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa
    28561 aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat
    28621 actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt
    28681 ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt
    28741 ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata
    28801 acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata
    28861 accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa
    28921 atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc
    28981 gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc
    29041 aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt
    29101 tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact
    29161 gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat
    29221 ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt
    29281 atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg
    29341 ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct
    29401 gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc
    29461 acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt
    29521 ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa
    29581 agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac
    29641 gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg
    29701 catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa
    29761 cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa
    29821 aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg
    29881 gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt
    29941 gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt
    30001 ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt
    30061 ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa
    30121 tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta
    30181 tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt
    30241 gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg
    30301 cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc
    30361 actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt
    30421 gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg
    30481 tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt
    30541 acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag
    30601 gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc
    30661 atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa
    30721 gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca
    30781 ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca
    30841 ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa
    30901 ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca
    30961 gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg
    31021 gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg
    31081 ccgataacag caccagataa aacagggcta aagttataaa cagctaaata tccagctgca
    31141 atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc
    31201 acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga
    31261 atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt
    31321 gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat
    31381 gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct
    31441 gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca
    31501 tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc
    31561 gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg
    31621 gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct
    31681 ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact
    31741 tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc
    31801 acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag
    31861 cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg
    31921 acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc
    31981 gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta
    32041 gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc
    32101 aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca
    32161 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    32221 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    32281 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    32341 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    32401 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    32461 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    32521 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    32581 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    32641 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    32701 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    32761 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    32821 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    32881 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    32941 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    33001 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    33061 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    33121 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    33181 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    33241 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    33301 tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    33361 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    33421 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    33481 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    33541 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    33601 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    33661 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    33721 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    33781 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    33841 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    33901 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    33961 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    34021 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    34081 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    34141 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    34201 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    34261 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    34321 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    34381 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    34441 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    34501 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    34561 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    34621 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    34681 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    34741 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    34801 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    34861 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    34921 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    34981 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    35041 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    35101 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    35161 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    35221 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    35281 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    35341 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    35401 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    35461 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    35521 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    35581 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    35641 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    35701 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    35761 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    35821 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    35881 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    35941 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    36001 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    36061 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    36121 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    36181 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    36241 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    36301 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    36361 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    36421 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    36481 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    36541 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    36601 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    36661 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    36721 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    36781 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    36841 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    36901 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    36961 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    37021 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    37081 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    37141 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    37201 ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt
    37261 tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta
    37321 ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac
    37381 tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa
    37441 acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact
    37501 attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt
    37561 tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag
    37621 atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg
    37681 caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga
    37741 tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta
    37801 ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga
    37861 acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa
    37921 cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac
    37981 ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt
    38041 ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag
    38101 tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat
    38161 gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag
    38221 gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga
    38281 tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca
    38341 accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga
    38401 tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg
    38461 catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga
    38521 tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc
    38581 ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc
    38641 caattggata acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag
    38701 tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa
    38761 ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg
    38821 tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc
    38881 ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt
    38941 gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat
    39001 ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat
    39061 tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag
    39121 tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca
    39181 ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc
    39241 ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga
    39301 tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca
    39361 atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa
    39421 agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt
    39481 ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa
    39541 cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac
    39601 aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc
    39661 tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg
    39721 tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat
    39781 tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag
    39841 tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc
    39901 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    39961 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    40021 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    40081 ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    40141 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    40201 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    40261 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    40321 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    40381 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    40441 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    40501 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    40561 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    40621 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    40681 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    40741 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    40801 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    40861 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    40921 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    40981 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    41041 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    41101 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    41161 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    41221 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    41281 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    41341 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    41401 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    41461 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    41521 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    41581 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    41641 tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt
    41701 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    41761 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    41821 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    41881 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    41941 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    42001 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    42061 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    42121 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    42181 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    42241 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    42301 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    42361 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    42421 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    42481 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    42541 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    42601 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    42661 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    42721 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    42781 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    42841 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    42901 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    42961 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    43021 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    43081 ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    43141 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    43201 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    43261 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    43321 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    43381 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt
    43441 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    43501 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    43561 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    43621 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    43681 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    43741 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    43801 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    43861 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    43921 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    43981 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    44041 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    44101 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    44161 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    44221 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    44281 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    44341 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    44401 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    44461 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    44521 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    44581 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    44641 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    44701 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    44761 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    44821 actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact
    44881 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    44941 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    45001 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    45061 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    45121 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    45181 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    45241 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    45301 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    45361 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    45421 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    45481 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    45541 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc
    45601 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    45661 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    45721 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    45781 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    45841 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    45901 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    45961 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    46021 aaaatatttt cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta
    46081 tttagataac tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg
    46141 aaacaatatt tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact
    46201 gcgaacgaaa attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa
    46261 tatcacaatg gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca
    46321 tctgtgctgc aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt
    46381 tcatctttgt tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt
    46441 tgaaatcttc gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca
    46501 attcaattag ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa
    46561 aaataatctt tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag
    46621 ctacgctgac tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt
    46681 gtttgccaat cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat
    46741 agcgctcttc gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct
    46801 cccaagtagt ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca
    46861 taggctggat ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact
    46921 tgccatagat gattcgatct actcggccac cactttcttt catgacatct attatttgag
    46981 tgaataactc cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt
    47041 tcaaaaagtc ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa
    47101 gcggcttatt tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat
    47161 ctactaattc gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt
    47221 ctttcagatt ctctgctaac agatttccag tagccatccc atggttacag acaacgactg
    47281 atttataaac ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga
    47341 caagaaaagc tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa
    47401 caaaatgagt gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg
    47461 gattcatgac ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga
    47521 gattttcgaa taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa
    47581 attggatcaa ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat
    47641 ctctagcatt gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat
    47701 tgtccaattg aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta
    47761 tttctgtatt tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc
    47821 agatcaatgt aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact
    47881 tattggaata gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga
    47941 ttttttgagc cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat
    48001 tctgattgat aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca
    48061 gtccttgctt tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc
    48121 gtctcatatc ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta
    48181 gatggatcgg gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc
    48241 gattcaaaaa gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct
    48301 tttccagctt caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac
    48361 tgctcaacca gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga
    48421 actcacttgc tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta
    48481 tttcaattgt tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg
    48541 cttacataat aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca
    48601 aacaaaaaat aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat
    48661 tacaaaaatt ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag
    48721 taataagaac aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat
    48781 cgcagatgca agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct
    48841 tgagcgataa gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat
    48901 tgccgatagg ctatctctgg cacaccgttt attcagaaaa aggagctgcg acaaggcttt
    48961 tgtcacaact ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc
    49021 ggcttgccat cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg
    49081 tcgttgtgtc ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca
    49141 gcatctgaaa tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac
    49201 ttcctgaata gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag
    49261 aactttctga tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt
    49321 ttcttcttcc caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc
    49381 tttcttttct ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt
    49441 ttgtctttta ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa
    49501 tgcaagaaat cccgtttgct cataactaga aggacctgta cctggaccat aatacccata
    49561 agaatctctg ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa
    49621 tgaacctctg cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc
    49681 gaatccttgg gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata
    49741 attgattttt ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata
    49801 gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt
    49861 cttatttagt cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct
    49921 atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg
    49981 ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta
    50041 gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg
    50101 catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat
    50161 gaatcattat ctagctcctt agctgcttga attgcaagcc tgatatgcgg ataccctaat
    50221 atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg
    50281 gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt
    50341 attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat
    50401 agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt
    50461 gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca
    50521 tgatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta
    50581 agaaataaag catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca
    50641 ggttttggac ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc
    50701 tgagtcactg ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat
    50761 tacttgctga aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca
    50821 aatgcacaac ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc
    50881 agccaactat ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac
    50941 gtacgataag cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca
    51001 atcattttgg aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg
    51061 atcgtattca agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa
    51121 cggacagaaa agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc
    51181 aaatattgat agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta
    51241 gcaacaaacc gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt
    51301 tgatagaaca gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca
    51361 tattttttat tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg
    51421 atctgcagca tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc
    51481 agtaaaaagc ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa
    51541 agataagccg tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa
    51601 aattcccaat aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata
    51661 aacattggtg ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt
    51721 gcgctgtggc tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca
    51781 gctgatactt tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct
    51841 ttctctctct ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt
    51901 tcaatagaat ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc
    51961 gagtcactac ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa
    52021 ttggcaattc aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta
    52081 ctgccggaat cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc
    52141 ctgataatat gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct
    52201 cttgtctata cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat
    52261 gttttgagat ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta
    52321 gtatttctgc gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa
    52381 cgacccattc aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat
    52441 gtgcttgaat cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg
    52501 tttcgacacg tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt
    52561 catttgtatt gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat
    52621 tttgttctga agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt
    52681 ttgtataatt attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt
    52741 cagcaaccga acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa
    52801 tttctttatt gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac
    52861 ctgaaatagc atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg
    52921 cataagcagc agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc
    52981 cgtatgcttg gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt
    53041 tatcttcttc gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa
    53101 cttcaccgtt tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga
    53161 tggcttcttc tatgaattta cttgatttac cataatacac agtactaata gaagaagttg
    53221 gatttttcat gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt
    53281 catttccttc tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt
    53341 cattgtaatc gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat
    53401 aaaatgctga gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag
    53461 cagctttgat tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc
    53521 gttgacgaga tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa
    53581 cgcctgcttc ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg
    53641 cgattaaata actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc
    53701 ctggaatcaa accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt
    53761 ctgaaataga taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta
    53821 caatgactgg aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag
    53881 caacgatttt tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag
    53941 cgattactac tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac
    54001 tttcagggac gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa
    54061 tcaattcacg tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa
    54121 aactattttt catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct
    54181 ggtgtagcac cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat
    54241 ctcgttaata cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca
    54301 gtagattctg ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact
    54361 cctacatgga ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc
    54421 atggatctct cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc
    54481 ttccatctgg gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga
    54541 atttcattgg tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg
    54601 cagctaataa tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca
    54661 cgatgccggc tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg
    54721 taacaccgcc aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca
    54781 aataagcaat tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg
    54841 gtgcattggc taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga
    54901 ctggtttttt atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc
    54961 ctaatggatc atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc
    55021 caggattatg tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg
    55081 tagaagagtt tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat
    55141 attgtcctgc agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa
    55201 aaaagacatc tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat
    55261 ggatgctttc aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata
    55321 cccagttctt cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca
    55381 attcgatagc tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg
    55441 cgtagattcc tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag
    55501 gatctcgtgt gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta
    55561 cccagtcacc agggatatct acacgtggta aagaatcgac aacttcgtta acttgaacga
    55621 tcacgatacc atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga
    55681 aacctgtgta caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg
    55741 gaatcaaatc gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt
    55801 tcattgtgcc gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga
    55861 aatcgatctt gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac
    55921 tagacatgat caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg
    55981 aaagaaaact tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa
    56041 tcagtttttc cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt
    56101 tgctgatttt ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg
    56161 gtttttccat aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat
    56221 gttggtctag attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct
    56281 ttgtcaaagt attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca
    56341 agtccatcat ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa
    56401 tctcttctgc gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat
    56461 cagatagatg gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga
    56521 gacagtgaga tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg
    56581 cttctgccaa cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac
    56641 aattttcatt tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct
    56701 cagcttcgct tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt
    56761 ctttcattaa gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc
    56821 gacaagcaac agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa
    56881 taccgttttt ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga
    56941 taaaaaccgt atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt
    57001 ctaaacgttc catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt
    57061 gtccaggaaa taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca
    57121 ctgctatctt agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac
    57181 aaaaaggtat ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa
    57241 gtattcgaaa caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc
    57301 ttgagccgtg cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc
    57361 ggcagaaaca ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca
    57421 ttagacacat tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact
    57481 tccgtcatat tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt
    57541 atggaaaagc atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt
    57601 accgatcctg tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc
    57661 atgaaagaac ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat
    57721 ctgcaattag aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc
    57781 cgtcatacac tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg
    57841 acagatgatc ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc
    57901 ccaaaagttt cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat
    57961 ttgcccttga cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc
    58021 aaaatgattg caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg
    58081 gacaaaattc ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta
    58141 gaaaataagc cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    58201 tcttgaagct aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt
    58261 tgatttaaaa cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag
    58321 atcaatagag atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc
    58381 tcgcaaaatt ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg
    58441 gtcacttgca tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt
    58501 tttgcttttt tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg
    58561 agacaaattg tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt
    58621 ccatctcttt cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa
    58681 tacaagtagt tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt
    58741 caatccgcct gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc
    58801 acctattttt tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca
    58861 gtaaatgctc cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt
    58921 attttctccg tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa
    58981 atcattaccg aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat
    59041 acaaagataa ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt
    59101 tctgaccttc aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa
    59161 gcaggaatca gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt
    59221 ttcaaacaat cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct
    59281 gtccctgatt ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact
    59341 aaaatcaatc agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct
    59401 tgtattcttg gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc
    59461 actcataaca actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt
    59521 ttaagaaacc tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt
    59581 tggttcttta tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat
    59641 accaacagca cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa
    59701 ggtatttatc ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg
    59761 aattacaaca ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta
    59821 gaggtcctcg aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa
    59881 aaaacatctc tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata
    59941 ttgattgaag cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat
    60001 gcacacttgt atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc
    60061 aatgaaccaa attactttct ccaactcttg tctctactga tccaacaaac gattttttct
    60121 atcaaaccga atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg
    60181 ttcttgcagc aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa
    60241 ctaaccgatc tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct
    60301 ctagattctc tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc
    60361 aatcgtttga ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc
    60421 tctagctgtt ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac
    60481 aactaagaat agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact
    60541 tactgtaccg atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc
    60601 aaggatacct aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg
    60661 cttggacaac tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag
    60721 aagaatggtt tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac
    60781 catgatcagt agggagacat agatgatccg aaacaaatga taaccagata ggattccttg
    60841 ctttggatct cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc
    60901 ataagataaa gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg
    60961 tagcgggcga ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg
    61021 caagaagaaa ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc
    61081 ttttgcaatc gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac
    61141 ttcactacct ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc
    61201 atttacgccg tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat
    61261 tcgcagttta tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat
    61321 caactcttca tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt
    61381 taagccaatt tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt
    61441 cactgagatc cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg
    61501 atcgatcaat ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc
    61561 ctgtttcgaa tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa
    61621 ttctgcagct gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa
    61681 taatacttct ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt
    61741 tgccatatat ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag
    61801 attggctgtt tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc
    61861 tgttttctgt tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca
    61921 ggcttcttct tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt
    61981 cttatctgaa cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat
    62041 gatcgccttt tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc
    62101 tggcaatcct tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaagaatatg
    62161 ccaatccata ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat
    62221 gccttgaaaa atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg
    62281 ttcgaccgat tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac
    62341 aacgacaccg actgcagaac cagattgtac aagcgttcct gaaaaaccca tatttttctg
    62401 gtcgccgatc gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc
    62461 tcccgtcaaa atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc
    62521 tggaacgaca tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg
    62581 gattttttct tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa
    62641 accattcaat gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat
    62701 cactacgaat aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga
    62761 ggcaatgatc aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg
    62821 ttttgtctgc tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg
    62881 ttcttcttgc gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt
    62941 caatctgtac cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg
    63001 gaagtttttt caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa
    63061 tgtatctggg cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat
    63121 acaaaccaga taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg
    63181 aaagcaattg aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc
    63241 cgtttctttt ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga
    63301 aaacaactcg ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa
    63361 aaatgtagac aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt
    63421 acgcctttct tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga
    63481 tataaaatcc tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa
    63541 atcattagat tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact
    63601 aaaggacttt cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc
    63661 gttcctatat agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc
    63721 ttttgcggat cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg
    63781 aataaaacca taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg
    63841 atctgtccaa aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat
    63901 cctataccca ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat
    63961 tccaattcac caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg
    64021 ctacctccgc ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt
    64081 gctgttaatt ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa
    64141 aatgcccact ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta
    64201 tttgctaaaa ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc
    64261 aaataaacga gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat
    64321 aatcctgttc caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc
    64381 cgtttcattg ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac
    64441 ttcctgttcc caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat
    64501 gacactgatg gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc
    64561 gacagaaaat ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct
    64621 ctttttctct ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa
    64681 tctcaaaact gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt
    64741 tacgagcatc aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat
    64801 tgagataatg acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt
    64861 ccaaaaattg aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc
    64921 tggtcctgag gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa
    64981 cagattcaca ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc
    65041 gatattattc aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa
    65101 aatattgacg aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac
    65161 tggattttcc aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat
    65221 gatcggcgca aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca
    65281 tgaaaaactg cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc
    65341 taataagagc atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc
    65401 gatttttgcc atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat
    65461 gacaactaat gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa
    65521 caacggagcg agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag
    65581 ccatgagaca acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc
    65641 acctttttgg ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta
    65701 caataatact gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg
    65761 aatatttcgt acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat
    65821 ttgctttgat gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt
    65881 ttgttttctt ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa
    65941 aacccagtat caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa
    66001 acgaacgatc caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg
    66061 aaaaacaggg ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt
    66121 ctcttgttat tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt
    66181 ccattgcttt ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag
    66241 gagtcatgat agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg
    66301 ttagacccat tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg
    66361 tagtatgaga agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat
    66421 cgaacatttt ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga
    66481 atacccaatt gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc
    66541 ctttaacagt aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg
    66601 aagccccaat caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc
    66661 agactgcggc ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat
    66721 ctggagcctt tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca
    66781 tatttttatg tttgattacg attcctccag cagcataagg gacatagccc atcttatgag
    66841 gatctatcgt gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt
    66901 caatcgttac gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat
    66961 acggcacaaa ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg
    67021 catcaacatg taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga
    67081 ttttatctac actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta
    67141 gaattgggat tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca
    67201 ttcgataatc ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat
    67261 ctaacgcctt catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt
    67321 ggatattttt tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa
    67381 agctttcggt catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca
    67441 tttttttcac tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac
    67501 gggcgtacca taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc
    67561 cgtctgtcat attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag
    67621 ttgccataga agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag
    67681 ctaataaagc aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat
    67741 gccatgggat cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat
    67801 ggtcgcgggt ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat
    67861 ctgaaggaat ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca
    67921 ttttataaac tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat
    67981 ctttcatttt tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat
    68041 ctgtaacacc tcttcactat agcgccaaaa atgagaattt tttttttata acatcaaatc
    68101 cacgtagatt tttacaaaca aaaataaata aagtcggatt tttctgataa ttcaacgcaa
    68161 aaaagcgaac aatttttcaa taaatcagtc ttctaaaata gataacctaa aaaaagcgaa
    68221 caaaca
//
LOCUS       DAZKSR010000004        61949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000004 DAZKSR010000000
VERSION     DAZKSR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.004"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..1119
                     /locus_tag="ACG8XG_000231"
     CDS             283..1119
                     /locus_tag="ACG8XG_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0772178.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            1183..1614
                     /locus_tag="ACG8XG_000232"
     CDS             1183..1614
                     /locus_tag="ACG8XG_000232"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772179.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(1676..2998)
                     /locus_tag="ACG8XG_000233"
     CDS             complement(1676..2998)
                     /locus_tag="ACG8XG_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0772180.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3152..4792)
                     /locus_tag="ACG8XG_000234"
     CDS             complement(3152..4792)
                     /locus_tag="ACG8XG_000234"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0772181.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5125..5595
                     /locus_tag="ACG8XG_000235"
     CDS             5125..5595
                     /locus_tag="ACG8XG_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0772182.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            5817..6920
                     /locus_tag="ACG8XG_000236"
     CDS             5817..6920
                     /locus_tag="ACG8XG_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0772183.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7204..7884
                     /locus_tag="ACG8XG_000237"
     CDS             7204..7884
                     /locus_tag="ACG8XG_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0772184.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            7974..9008
                     /locus_tag="ACG8XG_000238"
     CDS             7974..9008
                     /locus_tag="ACG8XG_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0772185.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9022..9357
                     /locus_tag="ACG8XG_000239"
     CDS             9022..9357
                     /locus_tag="ACG8XG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772186.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            9341..11821
                     /locus_tag="ACG8XG_000240"
     CDS             9341..11821
                     /locus_tag="ACG8XG_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772187.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            11877..13436
                     /locus_tag="ACG8XG_000241"
     CDS             11877..13436
                     /locus_tag="ACG8XG_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772188.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            13433..14797
                     /locus_tag="ACG8XG_000242"
     CDS             13433..14797
                     /locus_tag="ACG8XG_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0772189.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            14968..16038
                     /locus_tag="ACG8XG_000243"
     CDS             14968..16038
                     /locus_tag="ACG8XG_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772190.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16053..16721
                     /locus_tag="ACG8XG_000244"
     CDS             16053..16721
                     /locus_tag="ACG8XG_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772191.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            16741..17319
                     /locus_tag="ACG8XG_000245"
     CDS             16741..17319
                     /locus_tag="ACG8XG_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0772192.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            17720..18631
                     /locus_tag="ACG8XG_000246"
     CDS             17720..18631
                     /locus_tag="ACG8XG_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0772193.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(18719..19636)
                     /gene="rnhC"
                     /locus_tag="ACG8XG_000247"
     CDS             complement(18719..19636)
                     /gene="rnhC"
                     /locus_tag="ACG8XG_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0772194.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            19838..20275
                     /gene="zapA"
                     /locus_tag="ACG8XG_000248"
     CDS             19838..20275
                     /gene="zapA"
                     /locus_tag="ACG8XG_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0772195.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            20334..20882
                     /locus_tag="ACG8XG_000249"
     CDS             20334..20882
                     /locus_tag="ACG8XG_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0772196.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            20997..23357
                     /locus_tag="ACG8XG_000250"
     CDS             20997..23357
                     /locus_tag="ACG8XG_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0772197.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            23444..23758
                     /gene="trxA"
                     /locus_tag="ACG8XG_000251"
     CDS             23444..23758
                     /gene="trxA"
                     /locus_tag="ACG8XG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0772198.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            23940..25718
                     /gene="uvrC"
                     /locus_tag="ACG8XG_000252"
     CDS             23940..25718
                     /gene="uvrC"
                     /locus_tag="ACG8XG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0772199.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            25863..26762
                     /locus_tag="ACG8XG_000253"
     CDS             25863..26762
                     /locus_tag="ACG8XG_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772200.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            26755..28017
                     /locus_tag="ACG8XG_000254"
     CDS             26755..28017
                     /locus_tag="ACG8XG_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772201.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            28178..30076
                     /locus_tag="ACG8XG_000255"
     CDS             28178..30076
                     /locus_tag="ACG8XG_000255"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0772202.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(30406..31125)
                     /locus_tag="ACG8XG_000256"
     CDS             complement(30406..31125)
                     /locus_tag="ACG8XG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0772203.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    31315..31362
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            31462..31794
                     /locus_tag="ACG8XG_000257"
     CDS             31462..31794
                     /locus_tag="ACG8XG_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772204.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            31781..33784
                     /locus_tag="ACG8XG_000258"
     CDS             31781..33784
                     /locus_tag="ACG8XG_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0772205.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            33803..34273
                     /locus_tag="ACG8XG_000259"
     CDS             33803..34273
                     /locus_tag="ACG8XG_000259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0772206.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            34294..34878
                     /locus_tag="ACG8XG_000260"
     CDS             34294..34878
                     /locus_tag="ACG8XG_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0772207.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            34893..35879
                     /locus_tag="ACG8XG_000261"
     CDS             34893..35879
                     /locus_tag="ACG8XG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0772208.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            35869..36180
                     /locus_tag="ACG8XG_000262"
     CDS             35869..36180
                     /locus_tag="ACG8XG_000262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0772209.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            36202..37983
                     /locus_tag="ACG8XG_000263"
     CDS             36202..37983
                     /locus_tag="ACG8XG_000263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0772210.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            37976..39352
                     /locus_tag="ACG8XG_000264"
     CDS             37976..39352
                     /locus_tag="ACG8XG_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0772211.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            39359..39994
                     /locus_tag="ACG8XG_000265"
     CDS             39359..39994
                     /locus_tag="ACG8XG_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0772212.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40024..41379
                     /locus_tag="ACG8XG_000266"
     CDS             40024..41379
                     /locus_tag="ACG8XG_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0772213.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(41571..42965)
                     /locus_tag="ACG8XG_000267"
     CDS             complement(41571..42965)
                     /locus_tag="ACG8XG_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0772214.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            43057..44028
                     /locus_tag="ACG8XG_000268"
     CDS             43057..44028
                     /locus_tag="ACG8XG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0772215.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            44025..44807
                     /locus_tag="ACG8XG_000269"
     CDS             44025..44807
                     /locus_tag="ACG8XG_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0772216.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(44976..45347)
                     /locus_tag="ACG8XG_000270"
     CDS             complement(44976..45347)
                     /locus_tag="ACG8XG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0772217.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            45481..45924
                     /locus_tag="ACG8XG_000271"
     CDS             45481..45924
                     /locus_tag="ACG8XG_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0772218.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(45967..46776)
                     /gene="yidA"
                     /locus_tag="ACG8XG_000272"
     CDS             complement(45967..46776)
                     /gene="yidA"
                     /locus_tag="ACG8XG_000272"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0772219.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(46795..48162)
                     /locus_tag="ACG8XG_000273"
     CDS             complement(46795..48162)
                     /locus_tag="ACG8XG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0772220.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(48146..48976)
                     /locus_tag="ACG8XG_000274"
     CDS             complement(48146..48976)
                     /locus_tag="ACG8XG_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0772221.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            49139..49576
                     /locus_tag="ACG8XG_000275"
     CDS             49139..49576
                     /locus_tag="ACG8XG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0772222.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            49652..50272
                     /gene="rpoE"
                     /locus_tag="ACG8XG_000276"
     CDS             49652..50272
                     /gene="rpoE"
                     /locus_tag="ACG8XG_000276"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0772223.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            50461..50967
                     /locus_tag="ACG8XG_000277"
     CDS             50461..50967
                     /locus_tag="ACG8XG_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0772224.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            50984..51883
                     /locus_tag="ACG8XG_000278"
     CDS             50984..51883
                     /locus_tag="ACG8XG_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0772225.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            51970..52854
                     /locus_tag="ACG8XG_000279"
     CDS             51970..52854
                     /locus_tag="ACG8XG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0772226.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(53046..53117)
                     /locus_tag="ACG8XG_000280"
     tRNA            complement(53046..53117)
                     /locus_tag="ACG8XG_000280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53082..53084),aa:Arg,seq:ccg)
     gene            53177..54511
                     /gene="rpoN"
                     /locus_tag="ACG8XG_000281"
     CDS             53177..54511
                     /gene="rpoN"
                     /locus_tag="ACG8XG_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0772227.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            54660..55751
                     /locus_tag="ACG8XG_000282"
     CDS             54660..55751
                     /locus_tag="ACG8XG_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0772228.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            55792..56793
                     /gene="gap"
                     /locus_tag="ACG8XG_000283"
     CDS             55792..56793
                     /gene="gap"
                     /locus_tag="ACG8XG_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0772229.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            56903..58093
                     /gene="pgk"
                     /locus_tag="ACG8XG_000284"
     CDS             56903..58093
                     /gene="pgk"
                     /locus_tag="ACG8XG_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0772230.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            58197..58952
                     /gene="tpiA"
                     /locus_tag="ACG8XG_000285"
     CDS             58197..58952
                     /gene="tpiA"
                     /locus_tag="ACG8XG_000285"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0772231.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            59068..60366
                     /gene="eno"
                     /locus_tag="ACG8XG_000286"
     CDS             59068..60366
                     /gene="eno"
                     /locus_tag="ACG8XG_000286"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0772232.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            60765..61904
                     /locus_tag="ACG8XG_000287"
     CDS             60765..61904
                     /locus_tag="ACG8XG_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0772233.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        20466 a        10840 c        13060 g        17583 t
ORIGIN      
        1 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
       61 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
      121 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
      181 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
      241 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
      301 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
      361 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
      421 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
      481 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
      541 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
      601 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
      661 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
      721 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
      781 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
      841 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
      901 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
      961 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
     1021 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
     1081 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaggagta tactgtgata
     1141 aaaaaattca tttaaaaaaa taaaaattgg ctggtgattt ggatgttgcg ggaaatgcgt
     1201 ttaggagatg cagaagcact acgagaaatc aatgcggaac aattaggata tgatgtacca
     1261 ttaaccgtta cggtgagaca aatgaagaaa ctgttgaagg agccagaaaa aaattttttc
     1321 ttggtctatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
     1381 gaatcgattt tcagcgatcc gatgttcaat attatggcac ttgctgtgtc taaacatgct
     1441 gaaaaaagag gaatcggaaa ggccttgatg actggcgtag aagaagcgaa aagacgagga
     1501 atatcagcaa taagacttaa ttctgcagaa tacagagtag aagcacaccg attttatgaa
     1561 cgcatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct gtaaattttt
     1621 atcaaaaaat acctggtcat caagccgata cttgatgatc aggtattttt tttgactaag
     1681 aaagtaattg catcaagaaa cctgagatga tcaaaacgat ggcaccacca agccgattgc
     1741 ccatttgagc aaaagcaata agttccatcc gattagctgc agataagaca gctacatttc
     1801 ctgtaccacc catcgagttg ttgcataagc cagcagtaat agttgattcg attggatata
     1861 aaccaaacaa acgaccgaca aatcctgaaa ctactgaaat cgcgataaca cttgtcaaac
     1921 ataagacaac aaattgccat gtcaaagcag aagccaatac atttagattc aataacgcaa
     1981 taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagttg ttgaacatga
     2041 tagctgcatc ttcgtaatat tctggtaata ggtttgcgat tttacagata acaacgataa
     2101 tgatcatgaa tgcgtaagca tggactttag gaacaaaata gttacagatt tgtcctaaga
     2161 tgaagaaagt caatgatacc atcaaaccga cacctaattg tggaatggaa ggttttacat
     2221 ttctaggttt tccatcgcca aggtcgcctt tttccattaa tttaccatta ccgttgaatt
     2281 ttggcattga ttgtcccgca cgagcaacta acgctgcacc aatgatagct aggacatttg
     2341 tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcgtccgta cctaaaacat
     2401 ttgcataaat cgttgataaa ggtacagctc cggctccaac accaccagcc attgcgggca
     2461 atgagatata taaaacggaa tttgcaaaac catttccgat aagcatacct acgagtccta
     2521 cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga acagaagctt
     2581 tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca gcaatataaa
     2641 agtctaaaaa gcccatattg ctgacaaagt tttctgtgga ttttactaca tactcaggta
     2701 agatcccaaa tgtagcaatc gcggcagagg caaagatcgc aaaaacggag ccaccaccta
     2761 aataactttt gacgataggg atttttgttc cgataaaatg aaataaatta ccgaaaatga
     2821 ccaaaacagc gattgggcca atcatattcg tcggtaattt tcctaaagcc attgcaatga
     2881 tcaggacaag gaccatgatc aaataaaggg gcaaacttac gccggtaatt tttgttgacc
     2941 aaaacccttc ttttttcttt tcctcaactg cagttgcggt tgttgttttt tgtgccattt
     3001 ttacagatcc tttcttattc tgtatcgtat gaaaaaagcc aaagagaagc ggcaaaagag
     3061 cagtagttgg ctggctgctg ttttgccgca gtcgcttttt tctttttaga cgtcatacaa
     3121 agtttctgta tgttctttta ctataacaat cttaatcttc taaagattta tattcaggaa
     3181 cccatttagc agattcaaca gcttctttga tatccgtgat ttcttcacga ttcaattttt
     3241 gtgcaacaac tgattttgcg acagtttcag caatcgtttg agagaattca gtgatttttg
     3301 ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg cctaatgctc
     3361 gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca atcaaaccaa
     3421 gccctaaacc tgggtacatc aacgcattat ttgcttgacc gatttggtaa gttacaccgt
     3481 tatattccac atcagcagca gggatacctg ttccaactaa agcttttccg tccgtccatt
     3541 caatcaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa ggcatgatga
     3601 ttggacgtgg ggtatgagcc gccatttctt tcacgatttc ttcagtgaat gctccaggtt
     3661 gggtagaagt accgatcatg atcgttggat ggattgcttt tacaactgct tctaagtttg
     3721 tcaattcatc agcattagtg aattctgagc gtttacgagc gaatgggatt tgaccaggag
     3781 tcaatccttt tgtatcttca aacaataaac cctgtttatc tacttgatag aaatgttggc
     3841 gtgcttcttc ttcaggtaca ccttgacgga tcatttcatc taaaaggatg tttgcgatac
     3901 ctacaccagc agtaccagca ccaaatgtca agatcgtttg atctttcaat tgctcacctg
     3961 atatattcaa tccacctaat acaccagcta gtacaacgat tcctgtgcct tggatatcat
     4021 cattgaaagt agtgatttta tcttcgtatt tttctaagat cgttgcagca tttcctcgtc
     4081 caaaatcttc ccaatgtaat aacaattctg ggaataattc agtagctgat tgaacgaatt
     4141 gatcaatgaa ttcgtagtaa gtttcgcctt ctacacgagc atgacgattc cctaagtata
     4201 atggattatt caataattct tcgttattag taccagcgtc gattgataca ggcaagactt
     4261 gtgcaggatt gataccagcc gcagcagtgt agaccatcaa tttaccgata gcgatgtcaa
     4321 caccgttcac accccagtca cccataccta agattccttc tgcatctgtc acaacgatca
     4381 aacgaatgtc gcgtccatct gctgcgtttt tcaaacttgc tttgatattt tctggttcgt
     4441 cgattgaaag aaatgcagca tcttgtggtt tcaagaaaat ttcgttgtat tgttcgattg
     4501 aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt tcgcccatca
     4561 atttataaaa caatgtccgg ttcgtattga atagattcat taagaagata cgtttttcca
     4621 aatcggtctg tttgcttaag tattgaccgt aagcttggac agattgttgt tccaatgttt
     4681 ggacagtact tggaagcata ccagtgattc cgtattttgc ccgttcttct tttgtaaaag
     4741 ctgttccttt atttagaaaa ggattattta ataagtttag ccctgtaagc atgaatgatt
     4801 cctcctaagt tttaatacaa acgaagtata ggagtcagat gaggatatag tcaaaaagag
     4861 caggtataat aaattttatt tatatgagta atagagtcaa aagaaatcaa aatagcggta
     4921 tttgttcttt ttatatcaac aatccttgct tgattactat gcgtatctct tatatgctaa
     4981 agaaagaaaa gtagaagaat aaaggatatg ttagatagat aaatagaatc tctgaagaat
     5041 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
     5101 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
     5161 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
     5221 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
     5281 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
     5341 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
     5401 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
     5461 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
     5521 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
     5581 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
     5641 aaagtcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
     5701 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
     5761 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
     5821 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
     5881 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
     5941 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
     6001 ctcatgtatt aaaagatgta gaattacccg atacaactac acattttgac aaagaaacgc
     6061 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
     6121 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
     6181 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
     6241 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
     6301 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
     6361 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
     6421 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
     6481 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
     6541 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
     6601 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
     6661 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
     6721 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
     6781 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
     6841 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
     6901 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
     6961 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
     7021 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
     7081 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
     7141 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
     7201 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
     7261 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
     7321 ccaacaaata ccgtggagcc tcttaaccct actgatcctt caaaaccagt agagccagca
     7381 gatccggaaa atccagcaac tggccagaca ggtcctttaa cactggatgt agttcccgaa
     7441 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
     7501 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
     7561 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
     7621 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
     7681 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
     7741 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
     7801 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
     7861 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
     7921 ctataggtga aggatttttt accgcagttt ttgttgaaag gatgtggtgg acaatgaatc
     7981 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
     8041 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
     8101 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
     8161 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
     8221 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
     8281 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
     8341 tatcagcaca tgcagaaaca actgtaccta tagaagtgaa aatcccagca aaaagcttta
     8401 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
     8461 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
     8521 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
     8581 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
     8641 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
     8701 catttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
     8761 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
     8821 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
     8881 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
     8941 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
     9001 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
     9061 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
     9121 acattttatg atctaagtcc aattccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
     9181 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
     9241 aatgaacaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
     9301 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
     9361 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
     9421 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
     9481 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
     9541 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
     9601 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
     9661 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
     9721 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
     9781 gtatattgcc ggtgccccca tcaaggttac tagcggaagt ttattatcag caggaagcca
     9841 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattgacag atattattgc
     9901 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
     9961 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    10021 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    10081 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    10141 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    10201 aagtgcagag ataaatacgt ggaatagtta catctcacca agtgattcaa caggaaaata
    10261 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    10321 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    10381 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    10441 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    10501 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    10561 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    10621 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    10681 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    10741 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    10801 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    10861 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    10921 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    10981 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    11041 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    11101 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    11161 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    11221 agataccatt catcttactt acaactatca attaaatact gtagatactt ctttacaatc
    11281 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    11341 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    11401 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    11461 gaagacaagt tattatgaat caaaaggcga tttttctttt gatgtgcggg atactcggct
    11521 tccaagcacg tctccttggc aactgacagg tacattgact tctttattta agaatgatca
    11581 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa agcaattgat
    11641 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    11701 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    11761 acctggcgca acgtatcaag gcatggtcac atggaaactg acagctgggc caaccagcta
    11821 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    11881 gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg agtggacttg gaacttttca
    11941 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    12001 aaggagaggt ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    12061 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    12121 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    12181 atcattgtta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    12241 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    12301 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    12361 ggctgaccgg tccagaaagt caaattcgtt atttttattt ccaattattt tggtttggac
    12421 gtccctatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    12481 tgctgcaaaa agagtttagt tttgctatta cagaagaagg acgattgaaa tttgaaatat
    12541 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    12601 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    12661 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    12721 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    12781 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    12841 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    12901 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    12961 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    13021 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    13081 cccgctttga attatttgct cgctatgcat ctattttacg gacgctgatc aagcagttag
    13141 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    13201 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    13261 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    13321 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    13381 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    13441 cgtgatcatt ggtgcttccc atgcaggcat cacagctgcc ctaaatctaa gaaaacttca
    13501 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    13561 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    13621 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    13681 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    13741 ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc accttgtata aacaaattga
    13801 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    13861 aaaagaagca aaagaagtag tgatcttcgg tgctggttac attggattgg aattagcaga
    13921 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    13981 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    14041 aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    14101 cgtacaactt ctgtctcaaa caataaaggc agatatggtc attttttctg cccaaactcg
    14161 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    14221 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    14281 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    14341 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    14401 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    14461 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    14521 atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    14581 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    14641 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    14701 gtctgaatac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    14761 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    14821 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    14881 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    14941 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    15001 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    15061 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    15121 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    15181 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga
    15241 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    15301 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    15361 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt
    15421 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    15481 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa
    15541 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    15601 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    15661 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    15721 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    15781 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    15841 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    15901 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    15961 ttagtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    16021 ttgaaggcaa ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag
    16081 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    16141 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    16201 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    16261 aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta
    16321 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    16381 ataagattaa acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg
    16441 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    16501 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    16561 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    16621 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    16681 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    16741 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    16801 tttatgtcca agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc
    16861 acccagccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    16921 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    16981 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    17041 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    17101 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    17161 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    17221 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    17281 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    17341 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt
    17401 agtttttcat aaagacaaat ttgttattct ctcatgatta tcagggtata tttttattta
    17461 ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga
    17521 atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag
    17581 gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct
    17641 tttacagccc cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc
    17701 tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat
    17761 cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt
    17821 cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga
    17881 gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac
    17941 cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc
    18001 ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa
    18061 agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga
    18121 tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa
    18181 agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac
    18241 ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag
    18301 tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa
    18361 tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac
    18421 aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta
    18481 tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa
    18541 tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt
    18601 gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct
    18661 cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagcttttg aacaccgttt
    18721 atttttttag tagcttcaaa gctttttctg tatttgcaaa gtgaagtttg gcatattttt
    18781 caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc
    18841 cagctcctga tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa
    18901 agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag
    18961 taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat
    19021 ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt
    19081 ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt
    19141 ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt
    19201 tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa
    19261 caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta
    19321 gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat aattgactga
    19381 tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc
    19441 cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat
    19501 aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat
    19561 ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga
    19621 taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac
    19681 gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat
    19741 agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact
    19801 aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta
    19861 caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat
    19921 ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca
    19981 aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa
    20041 gaaacaagaa agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat
    20101 caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg
    20161 ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca
    20221 acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc
    20281 ttttatcaaa ataagaaaaa tttaaaaaaa gttagaagag aaaggaacgt agcatgctga
    20341 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    20401 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    20461 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc acgccaacat
    20521 caaagttagc attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag
    20581 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    20641 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    20701 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    20761 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgc tttatcgttg
    20821 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
    20881 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagattat ttaagaaggc
    20941 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
    21001 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
    21061 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagc gaggaacaaa
    21121 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
    21181 ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatt gagatcggag
    21241 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
    21301 tgaatcgttt cattgatgat ctttatgaca gtgaaatcga atttgcacgt ttgtatatgt
    21361 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
    21421 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
    21481 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
    21541 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
    21601 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt
    21661 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
    21721 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
    21781 gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag
    21841 cacgatttgg taaagaagta aaagctatcg tgccgggaat cagtgaagac aatcatgtcg
    21901 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
    21961 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
    22021 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
    22081 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
    22141 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
    22201 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
    22261 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
    22321 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata
    22381 cgattaatgc cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga
    22441 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
    22501 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
    22561 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
    22621 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
    22681 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg
    22741 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
    22801 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
    22861 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
    22921 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
    22981 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
    23041 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
    23101 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
    23161 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
    23221 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
    23281 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
    23341 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
    23401 aatgctatac tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
    23461 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
    23521 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
    23581 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
    23641 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
    23701 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
    23761 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
    23821 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
    23881 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
    23941 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
    24001 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
    24061 tccgctcata ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg
    24121 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
    24181 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
    24241 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag
    24301 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
    24361 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
    24421 tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct
    24481 atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt
    24541 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
    24601 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    24661 ctgatttact ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc
    24721 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    24781 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    24841 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    24901 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    24961 cgagtaagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    25021 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    25081 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    25141 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    25201 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga
    25261 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    25321 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    25381 caaaaaacga taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac
    25441 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    25501 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    25561 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    25621 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    25681 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    25741 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    25801 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    25861 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    25921 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    25981 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    26041 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    26101 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    26161 ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa
    26221 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    26281 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    26341 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    26401 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    26461 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    26521 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    26581 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    26641 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    26701 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    26761 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    26821 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    26881 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    26941 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    27001 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    27061 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    27121 atacaacaac aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca
    27181 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    27241 gattcaaaag gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt
    27301 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    27361 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    27421 caaaagcatt tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca
    27481 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    27541 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    27601 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    27661 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    27721 ggatacatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    27781 acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca
    27841 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    27901 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta
    27961 tggagttcat tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac
    28021 aatcctaaaa tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac
    28081 ctattttatt tattttacta tattggatat atattcattg aaaatagtgt ataacaactt
    28141 tataatattg agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact
    28201 ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat
    28261 tttgcagctt ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc
    28321 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    28381 ttctgaacaa atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga
    28441 atatgcatta acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt
    28501 gatcgatctt ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt
    28561 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg
    28621 aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa
    28681 atttttaacg aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
    28741 tggagatatt tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt
    28801 ttcttctgta gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg
    28861 ctctgaggca tgtttactga cacttttacg gattgcagca cgctataatt atttatttga
    28921 attagagaaa gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta
    28981 tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga
    29041 agaattagaa aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt
    29101 attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta
    29161 tgaagcatca acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca
    29221 aacgatccag ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag
    29281 tttgatgtat tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga
    29341 aaaaggggga atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt
    29401 agacgagaag ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
    29461 gctgggcttt tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga
    29521 tttagcaacc gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa
    29581 gagtaaaatg gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa
    29641 aagcaatcct tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga
    29701 attcgacaca aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa
    29761 aaaaggggag tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc
    29821 caaagcttat caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg
    29881 tttccaaatc gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc
    29941 cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga
    30001 tacaacaaaa ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta
    30061 tcgaaaggac gattgagaag aattccagtg aacattgttc catagactgg agactgagac
    30121 aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt
    30181 cacaagtcac actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact
    30241 cagagttgaa cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct
    30301 cggtattaag ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa
    30361 tactcgaaac taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac
    30421 aaagaaatcg aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca
    30481 ttgaacaccg aggattttat gcatatcatc tttgctttga accgcttcaa tgagtccatc
    30541 gttagaaaaa gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta
    30601 agaattcaca aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt
    30661 gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt
    30721 tggatataat gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag
    30781 tccccggcag actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg
    30841 ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta
    30901 aagttgtgga ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag
    30961 ttgaggagct gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa
    31021 aactgcatcc acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt
    31081 tgctcgaatc agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa
    31141 agtagactta ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata
    31201 cttatctgtt cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg
    31261 atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg
    31321 gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc
    31381 tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc
    31441 tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa
    31501 gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca
    31561 acagaaaaag agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag
    31621 ttggtggaag aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg
    31681 aaagcagaag cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt
    31741 caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga
    31801 aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg
    31861 aatccaaaac gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga
    31921 gaagtatttt ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa
    31981 tcggttgaaa gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca
    32041 aaagaaactc catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc
    32101 cgaagaaaat ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca
    32161 actaactgaa gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca
    32221 gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt
    32281 gagtgcagca aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat
    32341 ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc
    32401 agttgctgtt atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta
    32461 tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga
    32521 gcagcaaaag aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga
    32581 atgggtagaa gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat
    32641 ccatgcgact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt
    32701 gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc
    32761 aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga
    32821 accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac
    32881 accttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta
    32941 tggtttgctg atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg
    33001 gatgaaacgt ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt
    33061 gatctacagt tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc
    33121 tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt
    33181 cggtctgata cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa
    33241 agattacttg agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt
    33301 tattgcgttg gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg
    33361 gctatcgatc attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa
    33421 agtcaaaggt gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga
    33481 tttagtcagt tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc
    33541 attcaatatg ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt
    33601 gatcatcgct ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg
    33661 cgcacgattg cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt
    33721 tgatccgctg aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa
    33781 ataattggag gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt
    33841 atttgcggta ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg
    33901 tgttggaatg acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg
    33961 gcaggctctg attctgcaat tgcttccagg tacgcaaggt ttgtatggat tcgttattgc
    34021 ctttttgatt tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct
    34081 aggtgcttct ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt
    34141 cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat
    34201 ttttgctgcg atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt
    34261 gttgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct
    34321 cagatgaacg aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag
    34381 atcgatcaaa actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa
    34441 gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta
    34501 gaagtacgac aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat
    34561 gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca
    34621 ttgtattcat tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc
    34681 tatctttctc agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta
    34741 agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat
    34801 aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc
    34861 aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga
    34921 ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa
    34981 attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg
    35041 acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct
    35101 ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt
    35161 tccataatct gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt
    35221 atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt
    35281 caaatgaact tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag
    35341 agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc
    35401 gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg
    35461 acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca
    35521 tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag
    35581 ataaagatat ggaagattac acgaattttc tattagagac agattatgct gattttctgc
    35641 gccctgtcat ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt
    35701 atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag
    35761 cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa
    35821 gaagccattt cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag
    35881 atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt
    35941 cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag
    36001 tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt
    36061 tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg
    36121 atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag
    36181 tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt
    36241 gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta
    36301 ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa
    36361 gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt
    36421 gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc
    36481 tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac
    36541 catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga
    36601 gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat
    36661 gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta
    36721 tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta
    36781 agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag
    36841 cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct
    36901 tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg
    36961 gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc
    37021 cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct
    37081 aatacatcaa acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt
    37141 gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga
    37201 tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt
    37261 tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt
    37321 gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct
    37381 ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg
    37441 ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc
    37501 tattctcttt attcaacaga agtagggaag tatatggatc atatccttca agctgactgg
    37561 gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc
    37621 gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca
    37681 aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact
    37741 tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc
    37801 cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt
    37861 gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt
    37921 attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat
    37981 taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc
    38041 tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg
    38101 acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg
    38161 tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc
    38221 ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga
    38281 gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga
    38341 ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt
    38401 tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc
    38461 tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc
    38521 tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg
    38581 cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat
    38641 cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca
    38701 tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc
    38761 agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc
    38821 tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc
    38881 gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat
    38941 tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat
    39001 tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag
    39061 agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc
    39121 aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc
    39181 gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc
    39241 gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt
    39301 aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat
    39361 ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac
    39421 aactgccaca agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt
    39481 catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga
    39541 agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt
    39601 atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt
    39661 aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata
    39721 cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct
    39781 tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat
    39841 cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga
    39901 aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt
    39961 gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa
    40021 acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca
    40081 ttaggatttt ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt
    40141 aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt
    40201 gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg
    40261 atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt
    40321 gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat
    40381 ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg
    40441 atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg
    40501 aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat
    40561 ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct
    40621 gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat
    40681 cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta
    40741 ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg
    40801 aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga
    40861 tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg
    40921 atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt
    40981 gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc
    41041 cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg
    41101 ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc
    41161 atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga
    41221 ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga
    41281 cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca
    41341 aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc
    41401 tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt
    41461 gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct
    41521 tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca
    41581 tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta
    41641 gaggataggg aaattcgaac gaatgcatct ggttgcgtac gtgtagtaaa gcgtctggaa
    41701 tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca
    41761 ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa
    41821 agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta
    41881 tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc
    41941 acatttatat taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga
    42001 taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg
    42061 ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat
    42121 gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc
    42181 tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag
    42241 acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc
    42301 tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta
    42361 gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg
    42421 atagccaatc cattttgaac accggaaaca atagatagag attctaagga cggaatgact
    42481 ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct
    42541 tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca
    42601 acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt
    42661 acttcattgg tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt
    42721 ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt gccaacggta
    42781 gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc
    42841 tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga
    42901 cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata
    42961 ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat
    43021 tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa
    43081 gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat
    43141 tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa
    43201 ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca
    43261 aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag
    43321 attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc
    43381 acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat
    43441 gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt
    43501 tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat
    43561 acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca
    43621 gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca
    43681 tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact
    43741 cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat
    43801 tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta
    43861 gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat
    43921 aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt
    43981 tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt
    44041 acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc
    44101 tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg
    44161 aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc
    44221 gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt
    44281 acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa
    44341 gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat
    44401 tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt
    44461 tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta
    44521 tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact
    44581 ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag
    44641 cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt
    44701 atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa
    44761 taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg
    44821 aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag
    44881 acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt
    44941 ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta
    45001 taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag
    45061 tcagtaatct caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct
    45121 gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa
    45181 gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt
    45241 tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt
    45301 gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc
    45361 cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt
    45421 catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc
    45481 atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca
    45541 gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct
    45601 ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt
    45661 gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat
    45721 gagtattttt actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt
    45781 gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg
    45841 agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga
    45901 tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt
    45961 tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga
    46021 agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag
    46081 accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc
    46141 ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt
    46201 atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt
    46261 ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt
    46321 gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac
    46381 taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt
    46441 gtcatcgatc gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa
    46501 atcttcatgt gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag
    46561 tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg
    46621 ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac
    46681 agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt
    46741 accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt
    46801 tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa
    46861 taaatcataa tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc
    46921 atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat
    46981 caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg
    47041 ataaaaatca taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata
    47101 tccgaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga
    47161 aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat
    47221 aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt
    47281 gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag
    47341 ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa
    47401 aattacttcc ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg
    47461 gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc
    47521 ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt
    47581 gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat
    47641 ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa
    47701 accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc
    47761 tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc
    47821 atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca
    47881 gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct
    47941 tgaaatctca taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt
    48001 gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat
    48061 caaatctaag attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac
    48121 tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt
    48181 tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt
    48241 ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg
    48301 caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg
    48361 ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag
    48421 attgataaaa tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt
    48481 acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc
    48541 catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta
    48601 tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta
    48661 tggttatttt ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat
    48721 ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca
    48781 gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg
    48841 tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta
    48901 atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc
    48961 gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat
    49021 actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt
    49081 tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat
    49141 ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg
    49201 ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct
    49261 gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa
    49321 attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa
    49381 atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat
    49441 tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt
    49501 tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt
    49561 ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat
    49621 aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa
    49681 caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt
    49741 catggatttt tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga
    49801 aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc
    49861 attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga
    49921 agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa
    49981 agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga
    50041 agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga
    50101 tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga
    50161 tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga
    50221 tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt
    50281 ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga
    50341 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
    50401 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
    50461 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
    50521 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
    50581 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
    50641 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
    50701 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
    50761 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
    50821 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
    50881 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
    50941 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
    51001 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
    51061 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
    51121 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
    51181 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
    51241 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
    51301 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
    51361 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
    51421 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
    51481 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
    51541 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
    51601 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
    51661 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
    51721 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
    51781 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
    51841 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
    51901 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
    51961 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
    52021 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
    52081 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
    52141 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
    52201 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
    52261 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
    52321 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
    52381 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
    52441 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
    52501 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
    52561 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
    52621 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
    52681 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
    52741 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
    52801 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
    52861 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
    52921 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
    52981 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
    53041 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
    53101 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
    53161 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
    53221 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
    53281 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
    53341 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
    53401 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
    53461 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
    53521 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
    53581 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
    53641 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
    53701 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
    53761 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
    53821 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
    53881 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
    53941 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
    54001 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
    54061 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
    54121 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
    54181 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
    54241 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
    54301 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
    54361 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
    54421 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
    54481 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
    54541 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
    54601 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
    54661 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
    54721 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
    54781 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
    54841 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
    54901 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
    54961 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
    55021 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
    55081 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
    55141 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
    55201 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
    55261 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
    55321 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
    55381 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
    55441 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
    55501 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
    55561 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
    55621 tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg
    55681 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
    55741 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
    55801 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
    55861 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
    55921 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
    55981 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
    56041 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
    56101 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
    56161 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
    56221 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
    56281 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
    56341 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
    56401 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
    56461 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
    56521 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
    56581 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
    56641 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
    56701 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
    56761 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
    56821 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
    56881 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
    56941 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
    57001 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
    57061 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
    57121 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
    57181 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
    57241 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
    57301 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
    57361 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
    57421 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
    57481 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
    57541 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
    57601 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
    57661 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
    57721 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
    57781 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
    57841 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
    57901 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
    57961 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
    58021 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
    58081 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgaac aaaaggtgaa
    58141 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
    58201 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
    58261 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
    58321 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
    58381 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
    58441 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
    58501 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
    58561 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
    58621 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacag gtgtcaaaca
    58681 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
    58741 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
    58801 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
    58861 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
    58921 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
    58981 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
    59041 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
    59101 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
    59161 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
    59221 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
    59281 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
    59341 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
    59401 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
    59461 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
    59521 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
    59581 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
    59641 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
    59701 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
    59761 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
    59821 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
    59881 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
    59941 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
    60001 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
    60061 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
    60121 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
    60181 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
    60241 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
    60301 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    60361 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    60421 acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagttttttt
    60481 gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa
    60541 aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga acagtagaat
    60601 aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt
    60661 ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag
    60721 cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa
    60781 aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag
    60841 tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt
    60901 acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt
    60961 taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg
    61021 caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga
    61081 agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat
    61141 ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg
    61201 aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa
    61261 ccaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg
    61321 gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg
    61381 aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg
    61441 tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca
    61501 tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata
    61561 aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt
    61621 tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga
    61681 ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa
    61741 cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg
    61801 gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata
    61861 tgatcgaaaa atatcaagga attattttat cctacttagc gtaagatttt tttaataaat
    61921 gaaaaaacag agccagatta gcattattt
//
LOCUS       DAZKSR010000005        55502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000005 DAZKSR010000000
VERSION     DAZKSR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.005"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..3197
                     /locus_tag="ACG8XG_000288"
     CDS             558..3197
                     /locus_tag="ACG8XG_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0772234.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="ACG8XG_000289"
     CDS             complement(3365..4063)
                     /locus_tag="ACG8XG_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0772235.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="ACG8XG_000290"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="ACG8XG_000290"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0772236.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="ACG8XG_000291"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="ACG8XG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0772237.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="ACG8XG_000292"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="ACG8XG_000292"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0772238.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="ACG8XG_000293"
     CDS             9034..10140
                     /locus_tag="ACG8XG_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0772239.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="ACG8XG_000294"
     CDS             10157..11185
                     /locus_tag="ACG8XG_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0772240.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="ACG8XG_000295"
     CDS             11191..11670
                     /locus_tag="ACG8XG_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0772241.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="ACG8XG_000296"
     CDS             11686..12981
                     /locus_tag="ACG8XG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0772242.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="ACG8XG_000297"
     CDS             12992..13816
                     /locus_tag="ACG8XG_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0772243.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="ACG8XG_000298"
     CDS             13981..14880
                     /locus_tag="ACG8XG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772244.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="ACG8XG_000299"
     CDS             complement(15006..15875)
                     /locus_tag="ACG8XG_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0772245.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="ACG8XG_000300"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="ACG8XG_000300"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0772246.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="ACG8XG_000301"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="ACG8XG_000301"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0772247.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="ACG8XG_000302"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="ACG8XG_000302"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0772248.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="ACG8XG_000303"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="ACG8XG_000303"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0772249.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="ACG8XG_000304"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="ACG8XG_000304"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0772250.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="ACG8XG_000305"
     CDS             21273..22031
                     /locus_tag="ACG8XG_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0772251.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="ACG8XG_000306"
     CDS             22131..22775
                     /locus_tag="ACG8XG_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0772252.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="ACG8XG_000307"
     CDS             22782..23789
                     /locus_tag="ACG8XG_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0772253.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="ACG8XG_000308"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="ACG8XG_000308"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0772254.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="ACG8XG_000309"
     CDS             24667..25470
                     /locus_tag="ACG8XG_000309"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0772255.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACG8XG_000310"
     CDS             complement(25719..26783)
                     /gene="fni"
                     /locus_tag="ACG8XG_000310"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0772256.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="ACG8XG_000311"
     CDS             complement(26780..27865)
                     /locus_tag="ACG8XG_000311"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0772257.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACG8XG_000312"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="ACG8XG_000312"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0772258.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACG8XG_000313"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="ACG8XG_000313"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0772259.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="ACG8XG_000314"
     CDS             30108..30767
                     /locus_tag="ACG8XG_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0772260.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="ACG8XG_000315"
     CDS             30852..31757
                     /locus_tag="ACG8XG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0772261.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="ACG8XG_000316"
     CDS             complement(31759..32391)
                     /locus_tag="ACG8XG_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0772262.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="ACG8XG_000317"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="ACG8XG_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0772263.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="ACG8XG_000318"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="ACG8XG_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0772264.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="ACG8XG_000319"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="ACG8XG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0772265.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="ACG8XG_000320"
     CDS             34171..35508
                     /locus_tag="ACG8XG_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0772266.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="ACG8XG_000321"
     CDS             35556..37685
                     /locus_tag="ACG8XG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0772267.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="ACG8XG_000322"
     CDS             37660..38349
                     /locus_tag="ACG8XG_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772268.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="ACG8XG_000323"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="ACG8XG_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="ACG8XG_000324"
     CDS             complement(39506..39700)
                     /locus_tag="ACG8XG_000324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772269.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="ACG8XG_000325"
     CDS             complement(39750..40190)
                     /locus_tag="ACG8XG_000325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0772270.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="ACG8XG_000326"
     CDS             complement(40194..41039)
                     /locus_tag="ACG8XG_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0772271.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="ACG8XG_000327"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="ACG8XG_000327"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0772272.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="ACG8XG_000328"
     CDS             complement(42663..43610)
                     /locus_tag="ACG8XG_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0772273.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="ACG8XG_000329"
     CDS             complement(43833..44183)
                     /locus_tag="ACG8XG_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0772274.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="ACG8XG_000330"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="ACG8XG_000330"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0772275.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="ACG8XG_000331"
     CDS             45606..45926
                     /locus_tag="ACG8XG_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0772276.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="ACG8XG_000332"
     CDS             45948..46412
                     /locus_tag="ACG8XG_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0772277.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /gene="ytpR"
                     /locus_tag="ACG8XG_000333"
     CDS             46617..47222
                     /gene="ytpR"
                     /locus_tag="ACG8XG_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0772278.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="ACG8XG_000334"
     CDS             complement(47261..48550)
                     /locus_tag="ACG8XG_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0772279.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="ACG8XG_000335"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="ACG8XG_000335"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0772280.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="ACG8XG_000336"
     CDS             49542..50051
                     /locus_tag="ACG8XG_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFQ0772281.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..50801
                     /locus_tag="ACG8XG_000337"
     CDS             50151..50801
                     /locus_tag="ACG8XG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772282.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            51252..52190
                     /locus_tag="ACG8XG_000338"
     CDS             51252..52190
                     /locus_tag="ACG8XG_000338"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0772283.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            52203..53255
                     /locus_tag="ACG8XG_000339"
     CDS             52203..53255
                     /locus_tag="ACG8XG_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0772284.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            53521..54156
                     /locus_tag="ACG8XG_000340"
     CDS             53521..54156
                     /locus_tag="ACG8XG_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0772285.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            54347..55138
                     /gene="msrA"
                     /locus_tag="ACG8XG_000341"
     CDS             54347..55138
                     /gene="msrA"
                     /locus_tag="ACG8XG_000341"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0772286.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        17619 a         9922 c        11894 g        16067 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa ctaattaata
    50761 aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt tttcatgata
    50821 atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt aaataagtgt
    50881 attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc aacatcgtac
    50941 tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga aggttttagt
    51001 atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt tctatgaatg
    51061 tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat tctattccag
    51121 tttttattga tttgctgata aaataacttg aatggtggat ataaacatga taaataagag
    51181 aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga aagaaaaagg
    51241 aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt cgctttgggt
    51301 taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca gaacatgtcc
    51361 aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta gaagcaaaac
    51421 taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg attattctat
    51481 ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag
    51541 atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt gagaaatttg
    51601 taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt gaaggtcctg
    51661 gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt gatggaaaag
    51721 aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat gctcattttt
    51781 ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca atgaacggac
    51841 tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact gctcataaaa
    51901 tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag attgaactag
    51961 atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca attgggttac
    52021 attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa atcgattata
    52081 tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct tattgtgatt
    52141 tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa aggagagatt
    52201 gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc ctcgctggta
    52261 cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc attttaaaat
    52321 attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt tttcaactag
    52381 caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat ccgatgagaa
    52441 tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc acacctgatt
    52501 tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat acgatgctga
    52561 cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg tcagtgtcga
    52621 ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt tatttattcc
    52681 cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc actaccttac
    52741 aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt atctctccta
    52801 tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct ggtgcggctg
    52861 cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag gttaataagt
    52921 caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct aatctattta
    52981 gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct attcctgtcg
    53041 ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc ggacttgttg
    53101 gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt tgttggttag
    53161 tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta tttaaactgt
    53221 atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga aattttcaat
    53281 caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg cctttttgag
    53341 taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta aaatgtagaa
    53401 gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa tatgtgttaa
    53461 aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta ggagaagaaa
    53521 atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat tagtgattta
    53581 tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac taccgtaatt
    53641 agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg gtcagttgta
    53701 tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat tggaacatca
    53761 aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg tgattgtatt
    53821 tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc gacttggatc
    53881 cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt gttacttgtt
    53941 ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt tttaacagtt
    54001 attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat tagtcagttt
    54061 gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa ctatttgccg
    54121 atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga agctgattaa
    54181 gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa gaatagagat
    54241 gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag
    54301 tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt tagatgtgga
    54361 gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag aatgggaaag
    54421 aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc aggtgataga
    54481 aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac cagatgcgat
    54541 ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg attatgccaa
    54601 acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg gctgtttctg
    54661 gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat ctggttatac
    54721 aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata ctgggcatgt
    54781 tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc tgttggagat
    54841 ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc gggggaaaca
    54901 atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg aaaaatcaag
    54961 agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga ttcgccaagc
    55021 aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc aacctaaaag
    55081 atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac atagatagag
    55141 gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga caaaaatagc
    55201 ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga acaccgagga
    55261 aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg tatgacaagt
    55321 cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag agaattcgaa
    55381 ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata attaatagac
    55441 acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc aaacaataac
    55501 ta
//
LOCUS       DAZKSR010000006        43769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000006 DAZKSR010000000
VERSION     DAZKSR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.006"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACG8XG_000342"
     CDS             174..518
                     /locus_tag="ACG8XG_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0772287.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACG8XG_000343"
     CDS             519..938
                     /locus_tag="ACG8XG_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0772288.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACG8XG_000344"
     CDS             complement(1030..1380)
                     /locus_tag="ACG8XG_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772289.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="ACG8XG_000345"
     CDS             complement(1346..2677)
                     /locus_tag="ACG8XG_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0772290.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="ACG8XG_000346"
     CDS             2933..3499
                     /locus_tag="ACG8XG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0772291.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ACG8XG_000347"
     tRNA            3644..3731
                     /locus_tag="ACG8XG_000347"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            complement(3783..4988)
                     /locus_tag="ACG8XG_000348"
     CDS             complement(3783..4988)
                     /locus_tag="ACG8XG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0772292.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5126..5263)
                     /locus_tag="ACG8XG_000349"
     CDS             complement(5126..5263)
                     /locus_tag="ACG8XG_000349"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0772293.1"
                     /translation="MTLQPGEGTKQIAERAGITVDQLFALNGMDPNNYMLYPGQELRV
                     K"
     gene            complement(5565..6014)
                     /locus_tag="ACG8XG_000350"
     CDS             complement(5565..6014)
                     /locus_tag="ACG8XG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HFQ0772294.1"
                     /translation="MKKVITSLLVFASLLLFTACGKKVTTEDLKANDWLIEATKDDEP
                     NMIVSFSDHVVSFSVDTSSMKSTSSNDWEAMGEEFAKSLVDQMNYKFEYTLEGDVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKPNKSDDNEKLVLKPYKKEKN"
     gene            complement(6101..6301)
                     /locus_tag="ACG8XG_000351"
     CDS             complement(6101..6301)
                     /locus_tag="ACG8XG_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772295.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6330..6752)
                     /locus_tag="ACG8XG_000352"
     CDS             complement(6330..6752)
                     /locus_tag="ACG8XG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0772296.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6769..7191)
                     /locus_tag="ACG8XG_000353"
     CDS             complement(6769..7191)
                     /locus_tag="ACG8XG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0772297.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7482..7673
                     /locus_tag="ACG8XG_000354"
     CDS             7482..7673
                     /locus_tag="ACG8XG_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772298.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7684..7950
                     /locus_tag="ACG8XG_000355"
     CDS             7684..7950
                     /locus_tag="ACG8XG_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772299.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(8191..8670)
                     /locus_tag="ACG8XG_000356"
     CDS             complement(8191..8670)
                     /locus_tag="ACG8XG_000356"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772300.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            8715..9422
                     /locus_tag="ACG8XG_000357"
     CDS             8715..9422
                     /locus_tag="ACG8XG_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFQ0772301.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            9415..9543
                     /locus_tag="ACG8XG_000358"
     CDS             9415..9543
                     /locus_tag="ACG8XG_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772302.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            9601..9942
                     /locus_tag="ACG8XG_000359"
     CDS             9601..9942
                     /locus_tag="ACG8XG_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772303.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            9935..10681
                     /locus_tag="ACG8XG_000360"
     CDS             9935..10681
                     /locus_tag="ACG8XG_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0772304.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            10687..11373
                     /locus_tag="ACG8XG_000361"
     CDS             10687..11373
                     /locus_tag="ACG8XG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0772305.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11380..12213
                     /locus_tag="ACG8XG_000362"
     CDS             11380..12213
                     /locus_tag="ACG8XG_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0772306.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            12230..13081
                     /locus_tag="ACG8XG_000363"
     CDS             12230..13081
                     /locus_tag="ACG8XG_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0772307.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13078..13437
                     /locus_tag="ACG8XG_000364"
     CDS             13078..13437
                     /locus_tag="ACG8XG_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFQ0772308.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            13452..13613
                     /locus_tag="ACG8XG_000365"
     CDS             13452..13613
                     /locus_tag="ACG8XG_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0772309.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            13616..13915
                     /locus_tag="ACG8XG_000366"
     CDS             13616..13915
                     /locus_tag="ACG8XG_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772310.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            13915..14229
                     /locus_tag="ACG8XG_000367"
     CDS             13915..14229
                     /locus_tag="ACG8XG_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFQ0772311.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            14406..14717
                     /locus_tag="ACG8XG_000368"
     CDS             14406..14717
                     /locus_tag="ACG8XG_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0772312.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            14714..14932
                     /locus_tag="ACG8XG_000369"
     CDS             14714..14932
                     /locus_tag="ACG8XG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFQ0772313.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            14945..15226
                     /locus_tag="ACG8XG_000370"
     CDS             14945..15226
                     /locus_tag="ACG8XG_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772314.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            15223..15549
                     /locus_tag="ACG8XG_000371"
     CDS             15223..15549
                     /locus_tag="ACG8XG_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772315.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            15546..15743
                     /locus_tag="ACG8XG_000372"
     CDS             15546..15743
                     /locus_tag="ACG8XG_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772316.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            15740..15949
                     /locus_tag="ACG8XG_000373"
     CDS             15740..15949
                     /locus_tag="ACG8XG_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772317.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            15946..16233
                     /locus_tag="ACG8XG_000374"
     CDS             15946..16233
                     /locus_tag="ACG8XG_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772318.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(16266..16517)
                     /locus_tag="ACG8XG_000375"
     CDS             complement(16266..16517)
                     /locus_tag="ACG8XG_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772319.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            16825..17292
                     /locus_tag="ACG8XG_000376"
     CDS             16825..17292
                     /locus_tag="ACG8XG_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0772320.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            17591..17932
                     /locus_tag="ACG8XG_000377"
     CDS             17591..17932
                     /locus_tag="ACG8XG_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772321.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(17925..18116)
                     /locus_tag="ACG8XG_000378"
     CDS             complement(17925..18116)
                     /locus_tag="ACG8XG_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFQ0772322.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            18466..18678
                     /locus_tag="ACG8XG_000379"
     CDS             18466..18678
                     /locus_tag="ACG8XG_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772323.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            18685..18909
                     /locus_tag="ACG8XG_000380"
     CDS             18685..18909
                     /locus_tag="ACG8XG_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772324.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            18936..19469
                     /locus_tag="ACG8XG_000381"
     CDS             18936..19469
                     /locus_tag="ACG8XG_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFQ0772325.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            19456..20700
                     /locus_tag="ACG8XG_000382"
     CDS             19456..20700
                     /locus_tag="ACG8XG_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFQ0772326.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            20713..22212
                     /locus_tag="ACG8XG_000383"
     CDS             20713..22212
                     /locus_tag="ACG8XG_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0772327.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            22217..23143
                     /locus_tag="ACG8XG_000384"
     CDS             22217..23143
                     /locus_tag="ACG8XG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0772328.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            23270..23872
                     /locus_tag="ACG8XG_000385"
     CDS             23270..23872
                     /locus_tag="ACG8XG_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0772329.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            23885..24808
                     /locus_tag="ACG8XG_000386"
     CDS             23885..24808
                     /locus_tag="ACG8XG_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0772330.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            24826..25089
                     /locus_tag="ACG8XG_000387"
     CDS             24826..25089
                     /locus_tag="ACG8XG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772331.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            25101..25430
                     /locus_tag="ACG8XG_000388"
     CDS             25101..25430
                     /locus_tag="ACG8XG_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFQ0772332.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            25427..25753
                     /locus_tag="ACG8XG_000389"
     CDS             25427..25753
                     /locus_tag="ACG8XG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772333.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            25737..26075
                     /locus_tag="ACG8XG_000390"
     CDS             25737..26075
                     /locus_tag="ACG8XG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0772334.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            26075..26467
                     /locus_tag="ACG8XG_000391"
     CDS             26075..26467
                     /locus_tag="ACG8XG_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFQ0772335.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            26478..26990
                     /locus_tag="ACG8XG_000392"
     CDS             26478..26990
                     /locus_tag="ACG8XG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFQ0772336.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            27036..27404
                     /locus_tag="ACG8XG_000393"
     CDS             27036..27404
                     /locus_tag="ACG8XG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFQ0772337.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            27458..27745
                     /locus_tag="ACG8XG_000394"
     CDS             27458..27745
                     /locus_tag="ACG8XG_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772338.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            27762..30881
                     /locus_tag="ACG8XG_000395"
     CDS             27762..30881
                     /locus_tag="ACG8XG_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0772339.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            30893..31681
                     /locus_tag="ACG8XG_000396"
     CDS             30893..31681
                     /locus_tag="ACG8XG_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFQ0772340.1"
                     /translation="MDQRENKMYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGY
                     QTLTVEGREMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELL
                     NYLYRNEDVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVS
                     DGYIGNPIQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQV
                     TVNGEDCTSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            31678..34509
                     /locus_tag="ACG8XG_000397"
     CDS             31678..34509
                     /locus_tag="ACG8XG_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0772341.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            34484..36241
                     /locus_tag="ACG8XG_000398"
     CDS             34484..36241
                     /locus_tag="ACG8XG_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0772342.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            36258..37040
                     /locus_tag="ACG8XG_000399"
     CDS             36258..37040
                     /locus_tag="ACG8XG_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772343.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            37040..39226
                     /locus_tag="ACG8XG_000400"
     CDS             37040..39226
                     /locus_tag="ACG8XG_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0772344.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            39229..39675
                     /locus_tag="ACG8XG_000401"
     CDS             39229..39675
                     /locus_tag="ACG8XG_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772345.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            39677..39814
                     /locus_tag="ACG8XG_000402"
     CDS             39677..39814
                     /locus_tag="ACG8XG_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0772346.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            39851..40144
                     /locus_tag="ACG8XG_000403"
     CDS             39851..40144
                     /locus_tag="ACG8XG_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772347.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            40167..40364
                     /locus_tag="ACG8XG_000404"
     CDS             40167..40364
                     /locus_tag="ACG8XG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0772348.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            40375..41394
                     /locus_tag="ACG8XG_000405"
     CDS             40375..41394
                     /locus_tag="ACG8XG_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0772349.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            41651..42298
                     /locus_tag="ACG8XG_000406"
     CDS             41651..42298
                     /locus_tag="ACG8XG_000406"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0772350.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            42581..42654
                     /locus_tag="ACG8XG_000407"
     tRNA            42581..42654
                     /locus_tag="ACG8XG_000407"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42615..42617,aa:Ile,seq:cat)
     gene            43200..>43769
                     /locus_tag="ACG8XG_000408"
     CDS             43200..>43769
                     /locus_tag="ACG8XG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0772351.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT        15376 a         6996 c         9039 g        12358 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatatca acgattagac acgatttcga tcgtgtcttt tttgttgtat
     3781 atttagccta aaatgttttt tgactcccct tttgactccc cctgagtact gttcttcata
     3841 aatgctacaa aattattgat gacttttttg ttttgagttt cagttacatg agtgtaaata
     3901 tttgaagtag tttgaatgtc tgcatgccct aaacgttcct gaacatcttt taaagaagct
     3961 cctgattcaa aaaggagaga ggcatgagta tgtcgaaaac catgagtagt gattctttta
     4021 gtcaattctg gatgatgctt atatatgaca ttaagccacg aacgtgaaac tgtagggtta
     4081 tagagaagac catcatggtg aaaaattaag ttttcccttt ggattgtaac gatcgttttg
     4141 ttttttgtat aatactttct caataaactg atcaaatcat tgtccagata aatttttctt
     4201 tttccagatt ttgatttagg agtatttacg atcaatcttc cttgataacc tcttgttaca
     4261 gttttattga tattgagtgt tttgctctta aagtcaatat ccgaccaggt cagagcaaaa
     4321 gcttctcctt ttcttatacc tgtaaaagcc aatagggaaa agaataaata tctttcatca
     4381 tcatcgtttc ttattgcttc taaaaatttg attaattctt ctttagtata aaattcgata
     4441 tttttatctt cgatatctag agcttctcca cgtggaatag aaactaattt cataggattg
     4501 ctagttataa tttgtaaaga agcagcgtaa tcaaaaacat tagatgtgta attttttatt
     4561 ttcttaaaaa ttttaggatg gctatcggac caagtgttta ctgcttcttg acagaaaaaa
     4621 acgtctattt tgtcaatgaa cttatctcca aacactttta agatgtgtgt gtcaaaaatt
     4681 tgtttggtgg aggaccaagt gctttcttta actgtctttt tataattttc aaaccataag
     4741 ccgtaaacat attcgaattt cttgctcttt tgagctgtag aagcttgcag tcctttttcc
     4801 tgtatatctg cctccaatct ttttaaagct ctctcagcgg ctaatggcgt actaaaacct
     4861 cgtctcgttg tttttctttt ttttcctgtt agaggatcaa ctcctaagta cagactaaac
     4921 tgatattttt cttcgccttt ttttgttaag tgctttttaa ttcgtttatc aatttctttt
     4981 tttgccatct ttatctttcc tttcgtacgt ttgttcggtt gtacagcgga tttcgagatg
     5041 gtaaaatagg gtacaacaaa tagacctact ttaccgtagc tctttgcaca tttgcgttct
     5101 tggtcgggcg gcaaatgtgc tttttttatt tcactcttag ttcttgtcct ggataaagca
     5161 tgtagttgtt tggatccata ccgtttaatg caaacagttg atctaccgta attccagctc
     5221 gttcagctat ttgtttggtc ccttcaccag gttgcaaagt caaggtttcg gtttcagatg
     5281 aagaaggtgc cgttggttga gcttgattag ctggtacata atcttgtggt actgattgct
     5341 ccgttgaact gggagtgggt gaaacttgtt caacacttga actaggagta gcttctgcat
     5401 agttttctac actttcagca gagtaagtac tactagtttc aggaggcgta ttctgcgcaa
     5461 tagcagcttg ttctacagta ctagaagatg caatagtact ttctgttgta gtcgaagatg
     5521 attgttcgtt gctacttgtt gagctagtag ttgttctaga ttctttagtt tttttccttt
     5581 ttgtagggtt tcaaaactaa tttctcatta tcatcagatt tgttaggctt ttctggagtt
     5641 aaaataatat ttttatcttc tttgctgaca gtatatttgg catcattatc tttgtcttta
     5701 tcatctttcc aagtcataac atcgccttca agtgtatact caaatttgta attcatttgg
     5761 tcgactaagc ttttagcaaa ttcttctccc atagcctccc aatcgttact tgaagttgat
     5821 ttcatagaac ttgtgtcaac actaaaagat acgacgtgat cagaaaatga tacgatcata
     5881 ttaggttcgt catctttggt tgcttcaatc aaccaatcat tagcttttaa atcttctgta
     5941 gttacttttt taccacaagc agtgaaaagt aacagagaag caaaaactaa taaacttgtt
     6001 attacttttt tcatttttta ttcctcattt ctatgatatg atttttattg gggaatctta
     6061 gaaataaggt ttcgagtccg tgttgctgca cgggcttttt ttatttaaat aaatcccaaa
     6121 aactgaatgt agttttcttg tatactttat tgtaagcagc cttttttggg tctttaatcc
     6181 agccgctacc ttttttccca taaccaggaa taactgcttt ttttactttt ctttttgctt
     6241 ttccagtggt tctagcgctt atggattttt ttatacttgg ttttctcatt cctattttca
     6301 tctatatcct ccttacgttg tattataatt cacaagaaac gatcgaattc atctggtaat
     6361 ccaaagcaga cgagcttttc atattttgtc atatgatcaa agccgtctaa agttggatca
     6421 atagtcagat gagttgcgaa ataatttgct tctttttcaa tttttaattc agatactagc
     6481 gtttttttag acaacatagg tgtgttttca ttcggatgca aaacacagtg gcctaattca
     6541 tgactacaag ttatccattt tcccatctca tctaaactag aatttatgtg gatcattttt
     6601 atctgtttga atttactata gtaaccataa atttctccca aatcttcttc taaaactagt
     6661 atcttcaatt ctcgtgcaat tctaaaagga ttgcgtgttt gatatagttt taccagctta
     6721 ttaattttgt tatcaatttg aggtaaatac ataaacaacg tactcctttt aatcgcgata
     6781 ttttttaggt gtgtattttt tcttagcttc tattttagct attcgcaaag cattctctaa
     6841 agaaataatg agagcttcac gtgtcgcttc actcatttca ttatctttct tcgaaaaagc
     6901 cattccatca gcattagata aatcgtcgat taatgactgc aattcttttt ggatatcacg
     6961 ctcgtctttt tctgttaaat catagtatct ttttttctca gtacgtccta gcaaataatc
     7021 tacagatacg tcgaagtagt cagcaatttc ttgtaacttt tcggcagttg gctgttttcc
     7081 tgcgtttaaa ctataaaagt aattctctcc gtacttcaaa tctttagcga cttgttgaag
     7141 gtttttattc ttgtttttcg ctagttcttt tattctttca aatgttgtca ttccaacgtt
     7201 cctttccaac acgaaaaaat aatttacgaa aaagagtaaa aatagcttta caattttacg
     7261 atatagagtt attatttatc cgtaagttaa tttgatagaa aaaagcaaac taaaaacaca
     7321 ccttatagca taaagtttgg cgaccaagtg caaataaaag gttttgttat aggcttattt
     7381 ctctatgtat tcattttact ctatagtgta aaaatgtcaa tggaaattat attttttcta
     7441 tcattttaac ttacaaattt ataaaaaaag gaagtgggtt aatgtcaaac ttggataacg
     7501 gtcgtgcggc gatcaaagag tttatgaaag agaatggaat aactatggat gatttggcta
     7561 ctgcttatgg tcacacgcgt gtacgaatgc aacaaattct tgatggacac tggagtggac
     7621 caaaggcaaa cagaactatt ctagagatca tccgtgatta cagaattcga taggaggcaa
     7681 gtcatgaata ttctaagcga agagtttttg actcgtttaa gaattgcaat tgttgaagtt
     7741 gtaaaggacg cacttagtca actttcaaaa aagaatttgt cagaaacacg atatttaaaa
     7801 aagatcgaag ctagaaaata tgtgggaggt gtaaacgatc aaggttttga gaagttaata
     7861 gctcacggtt taaaagaaat tcgtatagat ggctttttaa gatatgacaa aaaggacatc
     7921 gatgaactga tggctaaata caaaatttaa aggaggtcaa catgggaaag tttaatcgaa
     7981 cattagtatt cagcgcgcca ctaattatct acgctttagg tctatggggt agtagacaag
     8041 cattaatcgg cacgatcgtt tacatgcttt ggattttcat tgggcttgat gaagctgagt
     8101 acagagcgaa aaagccagtc gggagggact gactaaaaaa gtctaatgtc tataagaaag
     8161 gtttgacaat aaatgaaaga attatttttt ttaataactt tcatcatttt ttctatcagt
     8221 gttttctaca ttaacctttg cgttgctaga tttgctttga aaagttttag gaacagcaaa
     8281 cgaaaataga gctataaaag atacgaatgt agtagctacg ttaaattgct ctttagaaaa
     8341 cgtcaatgtc attggaatga taattgatat cgatgagaaa atctttgcga taataactat
     8401 ccatttttca attataaaca tttttaaatt aagcaccatt agacaaaatg tagttcctat
     8461 atagaggata aatgaagata gaaataaaca tattagtcca gttttaaaaa aagagaagta
     8521 atctatgtgg gcaaattctg ttaatatgct tttttgattt aacacaatca aactactgtt
     8581 ggacaaaaat gacagtaagg aaagagttgc tccgattact aaataaaaaa tagttgttaa
     8641 caatattata ttatcaattt ttctgctcat gttttcaccc caccagaatt atatcaaaaa
     8701 ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtgactacga
     8761 gcttacaagt tgcagaaagc ttcgataaaa agcacagaca tgttttgacg gctattgatg
     8821 aactaaaaga gggggttgcc gaaaattggg cagacctatt ttgggaagat acttatgttc
     8881 atcctcaaaa taaacaatca taccgaatta tttatatgaa tagagacggc ttctctctat
     8941 tagcaatggg cttcactggt aagaaagctt taagttttaa acttcaatat attgaggcct
     9001 tcaacaagat ggaaaaagaa ttaaaagacc aattgccatc aattccagca actaaacgag
     9061 aaatggcatt acttgctcta gctgcaaacg aagaaacgaa tgaaagagta gacgctatcg
     9121 agtctgattt aaacgatttg aagaacaatc aacttctagc tgaaccagat taccgaacaa
     9181 tttctaatat ggttcgcaac aaaattagag ttatttgtaa tcagcagcat ttaaatagca
     9241 aagcaaaagc agaattgttt aaagacttga atggcggaat taagcgaatc actggagcag
     9301 tagctagaaa tcgtatcaag gcaaaacagt tcgatgatgt tatcgatttt attaacaatt
     9361 ggatgccttc tacagcgacg atgacaatta ttaagcaaat ggagttgtta gaggatgaat
     9421 agagctgaag cgctaaaaat aggcgtaata attgctaatc gctggtggag acacaataaa
     9481 ccaaacatcc taagccaaca acatattgat aagcaaaaag catggcaaca aataaaaaag
     9541 tgactcagcc gaccaaagca atgagtcaca aagaaaatac atctaaggag atgttaccac
     9601 atgaaaaaag aactttccac tctagatcaa tatttgactg atcctagttg gggcaaatcg
     9661 aatatcaagg aaacaagcaa tcgaaaaatc agacgtaatc ttttgacgga tgaagaacta
     9721 gcatgtgatc aagacgattt gggaaatttt gtgagtattt gggatcatgt ctatcttatc
     9781 catttatcga ggaagtccaa aaaaactgaa tatatctatg tcatcgaaga tggcttgatt
     9841 gatgcgctag aagagtatga cagagataac ttgattgata tctcttatta cggatcaggt
     9901 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaaaggaac aacctttgaa
     9961 aaatagaagt gataatacgc tttttaatac cttatacaaa ataaatgtga aagatgttac
    10021 tgaaaaacga aataacctca cttatctttc gtgggcatgg gcttgggcag aagttagcaa
    10081 agtgtgtgag gcagtagatt atgaaatcta tcatgatcca gaaacatatc tgccgtatgt
    10141 ctttgataag aaaacaggct atatggtttt taccagtatc acagtcaacg gagtaaagcg
    10201 tgacatgtgg ttaccagtca tggatggtgc aaacaaggca atgaaagatg agccatatac
    10261 ctacgaagtc aatgattatc agtggaataa cgaaacgaag aaaaaagaga ttgttggaaa
    10321 aatcgaaaag cgagttgaag cagcaactat gtttgatatc aacaaaacga tcatgcgctg
    10381 tcttgtaaaa aatctagcaa tgtttgggct agggctatat atatttgctg gcgaagatat
    10441 gccagaagat gtctcgatgc ttgaaccagc tactcaaaga agcaaaaagc tattcttgga
    10501 tgctttacaa ctggttgcta acaagtacga aaaatcaatt gatgaagcaa ttgttgcatt
    10561 gactgatgcg gcttctataa ccgctgatga cagtaaatgg accaagagag acttgggcat
    10621 tctaaaacga ggcgttaact ggattgaaga tcagtacaga gaagaaacaa aagagaagtg
    10681 atatgagtgt ttaaaccatt gatcgattca tattcagcgg ttctgaaaaa gttcaaagga
    10741 aaagacatag gtgcaacgat caatgaagaa gtgaacattg atcgactaaa gacgatgtat
    10801 gacggatatg atggcgatcg gattattgaa gttcgattta ttgatccacg tcgatttact
    10861 gtgcagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
    10921 ccaacggact tctggaagga attcttttac ttccgttttg aaggcgtcac agggcgcaaa
    10981 ataagcctca aagacgaatc gaatacaact gtgagtgatg ctaatgtctt agcaaatatc
    11041 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacca
    11101 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
    11161 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
    11221 gttgatcatt cagagtacac atttgcagca ctctgcagga ttcatcacac ggagaaacac
    11281 aatataggtg tgatcaattt caaaaataaa tatcaaatca aagggatcaa gttaaaccag
    11341 gaaacaatta agaaacttag aataggaggg taaaaaatat tgtctgacaa caaacgctac
    11401 tactatttaa aactaaaaga gaattttttc gatagtgacg agatggttct cttagaaagt
    11461 atgccagatg gctatattta ttctaatatt cttctcaaac tttatttaag aagtctaaaa
    11521 cacgaaggta agttgatgtt taatgacagg attccattta actctacaat gctcgcaact
    11581 attacaagac attctgtagg agtcgtagaa aaagcggtac aaattttccg tgatttgcag
    11641 cttattgacg tattagataa cggagcaatt tatatgtctg atatacaaag tttcattgga
    11701 aaatcctcaa ctgaagcaga tagaaaaaga gaatacagaa agaaaataga agaggcaaaa
    11761 cggaatttaa taactggagg acaagtgtcg gacaaatgtc cagacaaaac tacaccagag
    11821 ttagagatag agatagagaa agatatagat atagataaag aagaaaagaa aggtaagtat
    11881 tctgacgaac acttacgcct tgctaaaaaa ttgcaaagta atttaactga agattttcca
    11941 aaagaaatga acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt gatggaagaa
    12001 agagataaag cgtctataga agcgattgag tatgtgatca attggctacc tacaaatgaa
    12061 ttttggtttg gaaatattag aagtgctaag aaattgagag aaaaatttga gaagctcaaa
    12121 ttcgaaatca aagcagacaa gaataatcat aaaaagcaaa gtcaaaaact acagtacagc
    12181 aatcctagtg aatatgacga cttgccaatt taaaaaggag atgcatcaca tggaaagcct
    12241 agcaaatgct atggagaaac taataagaag agtattagtg caaagcggga aatgtccaga
    12301 atgtagcgaa cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa
    12361 accaacatgc atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga
    12421 acggatatat aacgacagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt
    12481 attaacagat aaaactttgt ttaaatgcaa aatggagaat tatcacgtag tggaccaaga
    12541 aacgaaaatt gctttagaaa gagctaaaag ctttgtaaat gatgtcttac tgaaccatcc
    12601 tgcacatttc attctatcag ggaaatcagg aagcggaaaa agccacttgt caatggcgac
    12661 agcttgggaa atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca
    12721 agagttatta gagcaaataa agttttctta taacaatgct gaactgagaa aagaaattga
    12781 aggatcgctt atagccgata tcaaaacaac tgatttggtg gtttttgacg atattggagc
    12841 tgaattaggt agcggggtat caaatagtag gcagtttaca aacaacacgt taaacacgct
    12901 cttagaagcc agacagaaca aggcaacgat catcacaaca aacttatccg gtcctgaact
    12961 aagagaagcc tacggcgaaa gaattgtttc tagaatattt aagaattcag aaggttatgc
    13021 gctgaaattc caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg
    13081 aataaatacc gtaataagaa aactgttcat cgaggtatca agtttgattc tatcgcggaa
    13141 gcagagtatt acgatttagc cttgtggcaa gcagaagcga acggctggaa agtaaaactt
    13201 caggaaagat ttgagctgat gccgaaattt gaactagatg gaaaaaagta tcgcaagatc
    13261 gagtatattc ccgacttcac attttataaa aacggcaaac ttgtcaaagt cgtagatgtc
    13321 aaaggaatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa
    13381 gtgccgttga tattagctaa aaaatatcgg aatacgttca aggaagagcg tttttaacga
    13441 ggtggtccat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcaacgtg
    13501 agattgacga tctggaacgg ataaaggcag tgatggtcaa tgaaacggcg agaaaggcaa
    13561 tcaatttgca catagtgaat ttaagaaggg aaattcgtag attggaggaa tgagcgtggg
    13621 taagaaagca gcaatgaaac gaatcattga actgacacat tctgagaatt ggcaagaaga
    13681 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcctaa
    13741 acggaaaacg ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag taacgggtac
    13801 agctgaacag ttatctgaaa ttactggact gagcaaaaac atcatctggg atagagctag
    13861 gagcttatgg attgattcaa aggggcgaca gtttaggtat gtggaggaga aataatggat
    13921 ctcattacac aatacagtga tatcatcctc aagaaaatca tgatgaagat tcagaaagat
    13981 aaaaaagcaa aagaacgagc ggaattagtt aagttggaaa tggctgaaac aggagcagga
    14041 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
    14101 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
    14161 catcaggatc gtttcaaatt tgtagaaaag tatagagaga tactgaatga gtatttcgag
    14221 gaggactaga tgtttaattc aacgttagcg tcaattcact ttttattcta tataacgata
    14281 tttgtagcta tattaattaa tgcgataaaa agcggttttt taacaccaat cggcacaatt
    14341 ttaacattag cttcatttgt tacaagttgt attatccaac ttaaatatat aaaggagaat
    14401 gaataatgga cgaactaatc acaaaagtag agcagtgggc taaagataag ggattggatc
    14461 aagctgattc cagcaagcaa atgttaaaaa cgatcgaaga gatcggggaa gttgccgctt
    14521 ctctagctag aaaagatgaa catggtttaa gagacggaat tggagatgta gtagtaacct
    14581 tgattatttt agctatgcaa aatgatatgg atttgtacga gtgtctgaac caagcataca
    14641 acgaaatcaa aggacgcaca ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt
    14701 tggaggacag caaatgatca gcctaacaat attttggtgt gtaattggcg gcttactagc
    14761 gatgttagga gcatacctca tttacgagag tgcaaaaggc aaaaaatata cagcagtagg
    14821 gattattttg tttgttgtcg gaattttagt tgcgattggt gcagaaataa atctttattt
    14881 aaacggtacg acagaagagt tgatgtggtt tttattgtgg acaaggagat aggaggacag
    14941 cgaaatgggc gatatgggcg aatactggaa agatattaaa ccgcatctaa aagaacgtag
    15001 aaaacaacat gtcagaaaga tgggcaattc agcaactaaa aatattgaaa ccttgggatt
    15061 tgaatttact cattatccaa acaatcatca atttgcaatt aatacccata atggaattat
    15121 tgactactgg ggaactacag gaacgtggat tgagcgcaaa acaaaaaaac gtggtaaagg
    15181 gatacgtagt ttgaggaaat atattaatca ggaggacagc aaatgaacta tacacaacaa
    15241 gaactgactg atttatgtcc aaaacatgta gctgagttca tcaataatga agtcctgcct
    15301 aaatatgcag atggtttaaa tacagcagaa aacgttacag attttatgat taatgatgct
    15361 attgatcgtt tgatattttt agaaatagat tgtattgctt attatcgtct acacgcagaa
    15421 gttgctttga ttgatccgta tattgcttta agccaaaatc gaaaaatact cgtagcctat
    15481 attgatacca aacagatttt agaaggcaga gatgtcgaga tagagatcga tgaggaggaa
    15541 gtgggatgaa agcatacaca atcaagtttt atggaaataa aacctatgta tacatcctaa
    15601 gcggtctcag acttgatttt agaggctatt caaaagaaga ccttttagaa tccttatatg
    15661 gttacggata tttaaatgaa gatgaactaa taagcttaga gaaatttaca aatgcttgga
    15721 cgattgagga ggaagcagaa tgaagattta tgtagtaaag ttcgggaata aattttatag
    15781 aagtgatgaa cgctctatag gggctaacac attattcatt gtagacatac tccaaagtgc
    15841 gagatggttt gatagcctcg aagaagctaa ccaagtttca cgacgacttg gtggattaac
    15901 acaagtatat gaactggtca ctgtcgatca tgaggaggta gaaagatgaa attaaaagac
    15961 ggattttacg ctagtagtca tggtatcggc ggtttaatgc tagatatgcc gacaaagaat
    16021 cctaaaaaac gtaagaaacc aaaattcaaa gtcggtgaca tggttcgctg cgaagcagaa
    16081 gggttcatct atccatttcg tggatatgta gaacacgtct ataatcactc agcgatcatt
    16141 cgtattgaaa acacgatgga atgtgataag tggttagcga aaagcaaaga gaatttagct
    16201 gtagcgagat tggtggatat gaaggctata taaacagcga aaaaatgtac ttctaagaga
    16261 aaaatttatt ttttataaaa ataggaaagt atcattatga aaaagaccac agagataaat
    16321 aaacctacca cataaaaaat gaatatttct tgcgattcaa tataggtata tttaacaatt
    16381 tgtacaataa aaaatactat aggtaaaaaa gataacatga ggagcttatt tgttctagag
    16441 tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa cgggaatcca aaataacagc
    16501 atgctccaca tagtcactca accatctcct atacgcttta ttatcaatag ataatatcat
    16561 ttttcgtaat aaagataaaa aaataagtaa ggcttctcat taaaatataa aaaagacagc
    16621 cgaccactgg ctgtccttag cagaatattg aaatagtgag ccatctgttt tccgccagat
    16681 agctcaacat gtatatgggt aacgagtgca acctcgctac ttgaaaagct tagcataagc
    16741 atgggtataa aaaaagccgg attcctccga ccttaattaa taattctgac acaattatta
    16801 tatcataaat ggaggaatca atggatggta cttttcgatg taaagaagta tgagacacca
    16861 gaggcaaaag atgtggatat ggatcgcaca aaacataatg ttggtgtttt tctttcagca
    16921 tatctgtcag ctagatgtag gataggtcaa cctcgggagc ctaaagtaac ggcatcttac
    16981 tccttggttc caccttctac agcaaataat acatttgaag cagaacgaat gatgattgag
    17041 agagaggaag cgcaagaaga atttgagtac ttgcacaaat tatttgttcg tggctattca
    17101 gcaattcagc atccgcataa acctgatgta acagaaaggc gcaagaagat attctatgat
    17161 cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac tagcgaagaa
    17221 tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt agaactggtt
    17281 gcttttaagt agccagtttt tacacttttt ataccctttg attacccatt tggtttccat
    17341 tttatacctt ttttatacca atcacttacc tggtaaatga tttattatga tagtgtcgaa
    17401 agattagtga taggtctgag acaaaataat aataaaagga acatcgtttt attattgttt
    17461 cacaattaag cttcgataga cagcaacgga aatattaaga ataaggatgt gaattccaac
    17521 tccttctaaa ttgttcttat tatctatcat ccgttgctgt ttattaattt atgtattgga
    17581 gggaaaacga atggaagtcg agcaaataaa gtatcaaggt atggatcggg aacggttgat
    17641 tgatattatt gagcaacaac gtttagaatt gatccaaaaa gaagctgtat gtaaagagta
    17701 caaaaaacat ttagaacaag taattgagta tcattcagta gaaaagtaca gatcagtggt
    17761 gcaaaaaaat agagcaacga gtcctgatcc tcgtcaagat gtatcgatta gtatcaagac
    17821 gccagtattt aattctgtta aatcagaaaa gctagaaagt atctcaactg ctttggtaga
    17881 aaaagcagct gaaatgaact ccctagcaaa tgaagttagt cgtcttattt agtcacatcg
    17941 ataacttgag agtctttggt gattccgttt tttatagaat taatggtttg ttcagcggaa
    18001 tatttagtca agtatgtttc gctagttgct actacttcat tgttatcgga cttaataacg
    18061 aagtaatatt ggccattagt tgcttctctt ataacaaagt acaaattttt caccaccttt
    18121 taatttattt cagcggacca ctcgctgata aataaaatta tacacttagt atttattttc
    18181 acaatattaa tttgtcactg tggcggaaag ggtagacgct aagcatgtgt gttaggtcaa
    18241 tgcttcggca accatgcaag gttcgattcc ttgccagtga ctttggcaac tgagggttgg
    18301 aaatgggcgc tcaaagtaca cgagcaaggc gaggtcgata gtaatcgatg gaatcggtgt
    18361 aggttgcttg atagaactat aactgcatct cgtttatgag gtgtagtttt tacatattag
    18421 atcactcatc gagtggtctt tttattttgg aaggggagta aaaaaatgaa cgaaaaccaa
    18481 ttaagagagt tgtttaaaac gaatgaagca aacaaaacaa tggaggcgac attctacgaa
    18541 actcaaaaaa gcttagcgtt aatcgcaaaa caagctaagt atttctacga tcagcttatt
    18601 ctgcaaggat ttaatgacgc acaggctatg gaatttatga tgcgaacctt ttctgccagt
    18661 aaccaacaga aagagtgata cgtaatgagg aactactggt atgtatcatt atcaaataaa
    18721 tatccgcaac caaacgcaga tgatccaatt agagttgtcc agtcagtcca gattaaaaag
    18781 aagtactcca tcattgaaat gaccagagaa gctacgccaa aagagattga taagtacaat
    18841 cttcgttact gcggccatgg atattttagt gagcagaaca tacagacaaa tattgagaga
    18901 tattgttaat aaaattgaaa ggtggtggct tgaatgtggt aaatttgaca ccaaaacaaa
    18961 aagcttttgc ggatgaatat ataaaaaatg gtggtaatgc tactcaagcc gccattaaag
    19021 caggttatag caaacgatca gcaagagtaa taggtaagga aaacctaact aaacctaaca
    19081 taatacagta tattaatgaa cggctaaatc ctatcgaaaa gaagcgcaaa ttaagtgccg
    19141 aggacgcttt aaatgaattg atagatattt ggcaaggaga agtacaaata agcgtgagca
    19201 agcaaataga ccgcttggag aaaaacaagg ttattaaaca tatgcaatat gaatatacac
    19261 cagatttaga aagcaaattg aaagccttgg atttgtattt gaagtataaa tcgctgttat
    19321 cacaaacgca attagaaaaa gctcaaacag aaataaaatt aatgcaagca aaattagaac
    19381 aattacagat aaactcagag cgttctaccg aagaaaaact tgatgagttg ttagaaaaga
    19441 ttagtggtga attagatggc actagttgat atttataacc caaagcaaat cgacgtgtta
    19501 aataaaacca ttaagaatga ttggtttatt actttattac atggagcaaa acgttctgga
    19561 aaaacaaaaa taaacaatga tttgttcttg tttgaattgc gacgtgtgcg aaagattgcc
    19621 gatgaagaag acattaagga accaatgtat atcctagcag gagtttcaag tgcaacaatc
    19681 caaaagaaca tcttacagga actatacaac atgtacagca tagaacctaa attcgataaa
    19741 catggaaact ttaagctatt tggcgttaag gtcgtacaag cttatacagg aaacattggc
    19801 ggtgttggcg caattcgtgg tatgacagca tatggcgctt atatcaatga ggcatcgcta
    19861 gctagacaag aagtatttgc tgaaatcgtt tcacgttgtt caggaacagg agcgagaata
    19921 ctagctgata caaaccctga caacccggaa cactggctaa agaaagagta tatcgacaat
    19981 tcaagcaaaa atattcaatc gttccacttt gaattagatg ataatacatt tttatctgaa
    20041 cgatatcgta acaatattaa agaatcaaca ccaagcggca tgttttatga tcgtgatatc
    20101 aaaggtttat gggtttctgc agaaggtgtc gtttatcgtg attttgatgc tagcaagcac
    20161 tacatccagt caaaagactt accacctttg agcaactttt attgtggtgt tgactgggga
    20221 tatgaacact ggggttcaat cgtagttata ggtgaaacgg atgacggaac agcttattta
    20281 atcgaagaac atgctactca atttgaagag attgattatt gggtagatgt ggcaaaaggc
    20341 attcaagagc gttacggctt acgagtacct ttttattgtg actctgcgag accggagcat
    20401 gtggctaggt ttgtaagaga aaagattaat gctaaaaatg ctcataaagc acggttatct
    20461 ggagttgagg aagtcgccaa gagatttaaa caagataaat tgtttatctg tcaagataga
    20521 gtgatgaaat ttcgggatga aatttatcaa tacatttggg acaagaaaaa aggcgagcca
    20581 ataaaagaat atgatgatgt gctagactcc gttcgatatg cgatatatac tcatgagctt
    20641 cttaagaaac caaaagttaa tgtcaacgaa aagattaaac gtattaaacg catgttttaa
    20701 ggagtgtgaa aaatggataa ggtaaacgag tttgaatacg gagctgatat acattattct
    20761 aatgacgtga acacaaatta tgtaaagttt agcgtagact caaatcttca ctatagattt
    20821 agctcagcag aagatttact taacgattta gatactttag cagcaatgat aaaacatcat
    20881 catgaatatc aggtaaaaag gctaagtgta ttagatgatt attataaagc tagaaataca
    20941 aatatcatgg ataaccgtag gcgcagagaa aaagaaaaag cggatcaccg atcagcacat
    21001 aactttggaa aagttctttg tacgtttgat gttgggtaca acacaggcaa tcctataaaa
    21061 gtgcaaatcg aggacacaaa tcaacaaaaa gaaatcgaag agtttaatac taataatgac
    21121 atagatgggt taaatgctga actctggctt gatatggata agtatgggag agcctatgag
    21181 attatctatc gagattcaga tgatacagat tatgttgatt tggctaatgt atttgaaacg
    21241 tttgttgtat atgatactac agtaaaacga gagcctattt tggctgtacg gtatcctaag
    21301 acaagattca acaaggatgc tgataaacag tacattcaac caatcgtata cacaaaagaa
    21361 aaaagtatca cttatgatga gacgacacta acagcaattg agttaaagaa tccccaggat
    21421 gaaccgcatg aatataaaga ggtacctatt acagagtatt ctcctaatcg ttttcggatg
    21481 ggcttgtatg aagatgtact atctttgatt gatctatacg atgcagggca gtctgatacc
    21541 gccaattata tgactgatct aaacgatgct cttctagtta ttagtggtga tattgaagca
    21601 gcaggactat ccacagagga cgccatcaag cagaaagaag cgaatatgct tttgcttgag
    21661 tctggaactg atgtgaacgg taataaaaca agtgtgactg caggatatat ttacaaacaa
    21721 tatgatgtga acggtgtaga agcatacaaa tacagagtac gcaaggatat tcacgaaata
    21781 tccatggttc ctgatcttac tgatgacaat ttttccggag tgcaatcggg agaagcaatg
    21841 aaatataaat tatttggatt tgaacaaatg acggcaacaa agcaaaggct attcaaaaaa
    21901 ggccttatgc ggcgttatcg tcttttattt agcctaaaat caagtatttc tgaaatggat
    21961 aactccgatt tgaaaggctt acgtgtaata tttacgccta atctacctaa agccattctg
    22021 gaagagttga aatctttggt tgatgctgga gctgaactca gtcaagagac gatcttagga
    22081 ctcgcttctt ttgttccaga tgtacaggca gagttgaaac gagtaaataa agaaacgcaa
    22141 aagcagattg gcatttttga ttcagatggt gaagaagtaa ttaacaacaa aaaagatgaa
    22201 acaggggggt gattaaatga actcccaaga atattggatc aaacgggaaa aggaatggca
    22261 aaagcaacaa attaaagatg ataaaaagcg catggcagaa attaaaagtc gcatgcaata
    22321 cgcacaagat gcgatacaaa aagaaataga cgcgcagtgg gacagtttct ccaatggcca
    22381 gaaaatcact cgtagcgaag cgatgaagcg tgctagtgaa atggatgtca aagcattcgc
    22441 tcgcaaagca aagaagtatg tcaaagagaa agatttttct cctacagcaa accaagaatt
    22501 aaagctatac aatcttacga tgcgtgtaaa tagattagag ctcttaaaag ctaatatcgg
    22561 gcttgaattg atttcactgt ttaatgaatt ggataagtac ttttcgaatg aattaacaaa
    22621 agctggttta gctgaattga agagacaagc cggtatttta gaaatgacta ttgcttcaag
    22681 tggatatgca aagctgatag aactagtaat aaacagctcc tttttgagtg atgacgtgtc
    22741 ttttagtgat cgcttatgga tgtatcaatc tgaattgaaa tcagaattag ataggttgtt
    22801 agtcagaagt ataacgatgg ggaaaaatcc caagcaactt gcatctaaat tggcagaata
    22861 tttaacagct gaaggacgag aaaacactaa gttcaacact caacgtttga tggtgactga
    22921 aacgactaga gttcaggtag ggattcaaga acgaagttat agagatgcag gcattaccca
    22981 gtacatctat atagcagaac caacggcgtg caaactatgt ataccgttaa ataatcaagt
    23041 ttttgatgtt gccgatatgc agccaggaag taacgctcct aatatgcatc cattttgtcg
    23101 atgcagtaca gcaccttata tagaacgaat atcaagccgt taacacaaat taacggcttt
    23161 ttattgtgcc ttcttacagc ttacaggcgt taaagagaaa gctattttcg gctgaccggc
    23221 gtaactggtc aaatttatcg ggtagcggcg taaccgtgga ggattaatca tgaaaaaacg
    23281 tttatttatg ccaatgaact tacaattttt ttctgaacca ggagatggtg gatctggtga
    23341 tgagggacaa caaggaaacc taccagctgg ctcacaagag acaccgcccg aagcaaaaga
    23401 agaaaacaat actggcaaaa cattttctcg tgatgaagta gcgaaaatga tcgctgctga
    23461 gacgaataaa gcaaaagcag cgtgggaaaa agaactagca gcaaaaaaag aagaagctaa
    23521 aaagctggca aaaatgaatg cggaagaaaa actacagcat gagttggagc aaaaagaagc
    23581 tgaaatcgct gaattaaagc gtggacaggc actatctgaa atgacgaaag aagcttctaa
    23641 aatgctgaca gatgcaaatt taccacacga tgatgattta cttggtctga ttgtttctga
    23701 tgatgcagat gccacaaaac aagctgtagc agtcatcact aactttgctt ctttgattaa
    23761 gagagaaaac gcaagacaaa caccaccaaa tgaaggtgga caatttacag catcgaaaaa
    23821 tactaaagaa acagtggcta aactagctgc taaaaatcga attatcaaat aggaggaaaa
    23881 cttaatgaaa aagaaacaac ttttaccaat gaacttgcaa atgtttgctc aaacatggga
    23941 tccagataat gtcttggtat atgaaacgaa agagggaaaa attcctgata aatataatac
    24001 gctcattttg agtgaagtta tggaaaattc taagatcatg cagttagcaa aatacgaaga
    24061 aatgactgac aaagaaaaga aatttgaata ctttgcagaa ggaccaggcg catactgggt
    24121 gggtgaaggt gaaaaaatta aaacgtctaa acctaaatgg atgcaagcta cgatgactgc
    24181 aaaaaaactc ggtgtcattc ttccggtttc tcgtgaatat ttaaattata aattatcaga
    24241 tttctttgag gagatgcagc caaaaattgc tgaagctttc tataaaaaat ttgatgcagc
    24301 tgccttatta aataaagaaa acccatttcc tcagtcacta gacggatcag ttattagtgc
    24361 ggggaatgtg gttgaaggcg gattgactta tgataatatc ctagccttag aagacaagtt
    24421 agcagaaaat gaattcgaac ctaatgcgtt tatttcaaac cgaaaaaatc gtacagaatt
    24481 acgttctgca gctcaaacag tcgggtcaaa tgttgagttt atttatgatc gctctactaa
    24541 tacaattgac ggattaccag tagtagacct taagtcttta gataaaggaa cactttacgc
    24601 tggagacttt aattacatgt tttatgggat cccatataat atttcattta agatttctga
    24661 agaagcccaa ttgtctactt taactaatga agatggaacc ccagttaact tgtttgagca
    24721 agaactgatt gctttgcgtg caacaatgga tgttggattt atgattgtaa aagatgaagc
    24781 atttgggaag atttccccaa aagcgtaacg cctgctaccg gtattgtgcc aaatcaaaag
    24841 acatggaccg gtaaagtagg cgatactaaa acatttacta tttcagctgt gcctgcagat
    24901 gctagcgatg cagctgctgt tgttgcagct actacagcaa cttcaagtga tggagctatc
    24961 gcaacagtga ccaaaaatga aaatggtggt tttgatggaa cgattgcagc agaagggtca
    25021 gcaacattca catttacttc tggagaattc actacttcaa tcaatgtgac aggtcaacct
    25081 gctagttagg aagtaaaaat atgatgattg cagatgatat taaaaaactt cttaaaggaa
    25141 caatagatga aaagcttgaa gttattgagc gaagaacgaa tgagcgtatg aaaaccttgt
    25201 taaatacgca agaagttcct aaagaatttg aaacagttgt atatgaagta tcgttgaaaa
    25261 gattcaatag aattggtcaa gaaggtatgc agtcatattc tcaagaaggt ttatctatgg
    25321 cttttcctga ctcggatttt tcagagtatc aaaatgagat tgatgaattt aagcgtaaag
    25381 atcaggaaga gttgtacaag ccaaagcgag ggaggtttaa atttgtatga gatttacaga
    25441 tgaaattata tttgttaaac gttcatctga ctctaaatat gatccagatc tcggtgagtg
    25501 ggttgaaggc aaaccagaaa gaacaagaac agaggcaaac gtgacagata ttggcactga
    25561 tagaagtgtg actatttttg gtagtgtgga agaaggggcg aaggtcatta ggacgcagcc
    25621 tcttttttct atccctacat ttgactatat cgagattgaa ggaaagactt ggcaacaaaa
    25681 aacagctaga aatccagcat atagaaatag tttaattgtg caagaggtgg ttcttgatga
    25741 aggcacaact tgaatataaa ggaatcgatc agctgatgcg acatctgaaa aaagcagcaa
    25801 cgcttaatga cgttcaaaaa gtcgtgaaaa gtaatactgc tgaaatgact gaacgaatgc
    25861 aaaaaggtgc gccagtggat acaggttact tacgaagatc aataaacatg aatcttttag
    25921 aagctggttt aactggtatt gtaggaccga cagcagatta tgcaccttat gtagaatatg
    25981 gcactcgatt tatgtcggca cagccttatg ttagaccagc gtttaattac caaaaagtca
    26041 aatttatggc tgaaatgaaa gccttggtga aatgatgatt aagacaagag atcaatcaat
    26101 ttttgatgaa ctttttaaaa tatcccaaaa caaacttgga tataaaacat acgattacaa
    26161 aactttagag gatgttggtt atccttttgt ggaatttgag aacactcaga caatccatga
    26221 agtaaataaa actgacatta aagggtctgt gattgtggtt ttatccgttt ggggattaca
    26281 gaagaaacga aagcagttgt cagatatggc atctgctctt tttaatgaag ctagattgat
    26341 agaagccaca gaaggctatt attgggcttt aaattatcaa gcaagtggaa ttcaagtgat
    26401 ggacgacaca acaacgaata caccgctaaa acgggcggtt gtcacacttg aatttagaat
    26461 tagataggag gaagaacatg gaagcattaa aaggtattga tgtcattttg ctttatcgct
    26521 tattgaaaaa agaaactcag gaagccgctt ggaaaatggc atttcaaaca gaacacgaaa
    26581 atggtttatc aagagattca gactctacag tgacaaaaga tggaaacgtt caaagtttaa
    26641 gcccggttga atatgatttt tcggctactt caatagttgc taaaggagat tctcatgtag
    26701 atgaaatgaa acaagcttta ttaaatgggg atatcattga aatttgggaa attaataaag
    26761 cagaacaggg aacagatgat aatgcaaata agtacaaagc tacttattac caagcatatg
    26821 tgtctgaatt tactccatcg gctgctgcag aggataacgt tgaattaagt ttatcatttg
    26881 cagtaaatgg tgttggtcaa gatggttatg caaccttgac agaagatcaa gccgatgttg
    26941 ttcaatatgc attcaaagat accgtgaaag caacttcgac aggagcataa gagggcttag
    27001 atgctctctt ttttatttta ggaggatgaa aaacattgaa attaaaaatt aaaggtaaag
    27061 aatattcgtt taaatttggc actaaatttg tacgtgaatt agataaagtg atgcctttcg
    27121 tcgatggaaa tatggaattt ggaatgggac tctccgcaaa agtcttacca gagctacgtt
    27181 cttataacgt taacacgtta tcacgagttc tggaaatagc aaatagaact gaagatgaat
    27241 ctattacatt agatgaattg gatgattaca tcgatgaagt taaagacatt gaaaaattgt
    27301 ttgatgaagt cctaaaagaa ttggcggagt cgaacgcggg aaagttagcg gtccgaaacc
    27361 tgaatcagaa attgaaagaa gcggaaaaac aacaagtgga atagattctg cactggcata
    27421 cgaacaaatt cttatcaatt cttttcgata tttgggaatg accaatatct cagatatcga
    27481 aagaatgacg ttatatgaat acaacattcg tatgactgca gcccagttat cttggcttga
    27541 caaagaaaag ttgattcacg aattagcgtg ggcaaatcag caagtccaag cggagaaaaa
    27601 agtaggcaaa aagacagttc ctgtatatcg atcctttgaa gaattcttca attatcaaaa
    27661 aatcgaagat tcaatcatgg gagtttccga actttcaaaa caagataaaa aattccaaag
    27721 cttactaact aaagctaact cttgaggaaa ggaggagaat catggaacaa ttttctgttg
    27781 aagccttatt aaaagccaca gatagtggat ttgtaaagac ttttaaagat gcacaagatg
    27841 ctgttaaaac ttttgaaaag aattcaaata gtatgacaac cgctgttggt aaagtgatgc
    27901 aaggtactgg tgccgcaatg acaaagtata ttaccactcc tcttatagga gtaggcgtag
    27961 cagctgctaa agttggtggc gacttcgaag cacaaatgag tcgtgtaaaa gctatatcag
    28021 gagcaaccgg cgacacattc gaacagatga aacagcaagc gattgatcta ggagcaaaaa
    28081 ctgcttttag cgcaaaagaa tcagctgctg gaatggaaaa cttagcttct gctggattta
    28141 gcgcacaaga aatcatgaaa gcaatgccgg gtcttttaga cttagcagct gtatctggag
    28201 gggatgtggc tctagcttct gaaaatactg ctactgcttt gagaggattt ggtttagaag
    28261 caagtgaagc aggacatgtc gctgatgtat ttgctcgtgc tgctgcggat accaatgctg
    28321 aagttggaga catgggagaa gcattgaagt atgttgctcc tgtagctaat tcaatgggga
    28381 tttctttgga agaaactgca gcagctattg gtattatgag tgacgcaggc attaagggtt
    28441 ctcaagcagg tacaacgttg cgaggagcat tgtctaggtt agcaaggcca acaaaggcta
    28501 tgcaagatac aatggataat ttaggtgttt cgttttatga tgctgacggt aaaatgaaac
    28561 ctttaaaaac tcaagtagaa ttacttaaaa aagcttttga aggcctgacg cctgaacaac
    28621 aacaaaatgc tttagtaaca ctatatgggc aagaatcatt atcagggatg atggctttga
    28681 ttgataaagg acctgattca ttgggcaaat taacaaaatc tctgaaagat tctgatggtg
    28741 cagctgacga tatggctcgg accatgcaag ataatatgaa ttcttccatc gagcaaatgt
    28801 ttggagcttt tgagtcagca gctattgtaa ttcaaaagat tctagcacca tccatcaaaa
    28861 aagtagcaga tgccatatcc ggcttagtag aaaaatttgt aagtgctcca gaatcaactc
    28921 aaaaattagt agtggccata ggagcaattg tcgctgctat aggaccgtta atttttatga
    28981 ttggttcagt aattatatgg atcaataggg tgaaagtagc ttttaaagct ttaagtgaaa
    29041 gttcaaaatt gtttagtgga ttaagtaaag caatgggtct tcttacaaat ccggtttttc
    29101 tggttatagc tgcggtagca ctactcgttg taggtttcat ctatctttgg aatacgagtg
    29161 aagattttag aaacttttgg attggcttat gggagggaat caagtctgct gtaagctcgg
    29221 cagtagaatg gattcagaat gcatggaaat ctacaggaga atggtttaac aatttatgga
    29281 agtccattaa agaaggcgca gacaatgttt ggactacagt tcaagaagcc cctgggaaag
    29341 cggcagattg gattaaaaac aaatggactg gaacaaaaga gttcttttca aatttatggt
    29401 caagtattgc aaactctgct tcagagattt ggaatagttt aaaagaaggt gtcatatcag
    29461 ttattgatga tttagtttca agtgctggtg aaaaatggga agggtttaaa aatactatat
    29521 ctactgcatg gaaaacaatt acaagtaaaa tcaaatctgg ttttgatttt atactaaaat
    29581 atattggtcc atttgtaagt agcttttcag atgtgttctc taatatagtg aaagcaataa
    29641 caagtatatt tgctgaggtt aaaaacataa tagtaaatgc ttgggaaatc attaagtctt
    29701 taatagctgc gccactactg tttattatag atttaattac tggtgacttc gaacaaatga
    29761 aagaggattt agatctaatc tggaacacac ttgtccaatc agtggtaaat atttggacat
    29821 ctgtaaaaaa tatatttacg gaatatatcg gtgcaatagt aaatagtgcc gttagtttat
    29881 ggactggatt catacaaagt atttctaata tttggaatga agtagtttat caagcgacta
    29941 tgatttggat tgatttgaaa ctatttttta ctaatttatg gattgatatt aaatacagtg
    30001 caattcaaat gtggataaat ctaaaattct ccataattca aacttggatt gatacaaaat
    30061 atggtgcaat tgaactttgg aataatctaa aacaatggtt tttccaaacg gttaataata
    30121 tcgtgcaaac tcttataaaa agttggaaca gcttaaagca aggaacgata gatttattca
    30181 acaatacagt tcaaggtgct aaagacattt ggacttcatt caaatcttgg attggtgatt
    30241 taattactgg aaccaaagat aacatcattc agggatggga aaacctaaaa caaggtacta
    30301 tagatacttt caacaattta gtaaatggtg ctcaagaggc atgggataat ttagtaaatg
    30361 ctgttagtga cacggttgat agagtaactg gctggtttga taacttgaaa aatatcgatt
    30421 tactagcagc cggaaaagct atcatggata gttttctaga agggttacaa aatgcatgga
    30481 aatctgtgca agattttgtt ggaggtattg gtgattggat tcgtgaacac aaaggaccta
    30541 tccaatacga tagaaagcta ttgattccag ctggtcaggc tattatgaac ggtctgaata
    30601 aaggtctgac aggaggattc aatgacgtac agaatactgt tggaagtatg gcagacttta
    30661 tcgcggaact tttcaatgca aattctgatg tagatatagc tgcaaatttg aaaaatgcaa
    30721 ataaaaacat tggtgcacaa gttgaacata aaataaatat gggtggctct actaaaccag
    30781 ctgtatttaa aatcaatctt ggaagacaat cgtttagatt gtttatggac gatatttcac
    30841 aagctatggg cgaaggtgca gatattaatc tggaatttta ggagggaata ttttggatca
    30901 acgagaaaat aaaatgtact cattcaaaga tacaaccatt aatctcacta gttctaaacg
    30961 attccttccg acgtctgcca tgatgtacga tggaatgtat ttagaagatt tgattgatgg
    31021 ttatcaaaca cttacggtgg aaggtagaga aatgctttct gtagaagttg aacagcaaga
    31081 gatacaaatt ggttcaatca ttacaaatca gaaaatacct tcaagaacac taaaaataac
    31141 atataagctg gaagatagag atccagaaaa actacagttt aaattcaaag aactgttgaa
    31201 ttatttatac cggaatgaag acgtggaaat taggtttcat gatgaattag attattatta
    31261 ctacggtcgc tatacatcaa ctgatactgt tccaggagac tccaactcga ttatttcgag
    31321 ttttaatgta ttctgtgcgg atccactaaa gtatacgaaa gagtgtgtta gtgatggcta
    31381 tattggaaat ccgatacagt ttcctataac accaagaaaa attgaagtta ctttatccat
    31441 gaataattca atcaaaatta caaacggaga acaaaatatc acgataactg acgcggcaat
    31501 aaaaacagga gacgtgttgg tttttgattt ttccgatgag caggtaactg taaacggaga
    31561 agattgtact tctatgattg atttagaaag tgattttgag aacttttatc ttaagcaagg
    31621 tcagaagata actagcaata atgggaagct taaaatattc tatagggggg cgacaatttg
    31681 agtgagacag tttatttctt tgatcacttg caaaaactta ttaaaagaaa aaatacaaga
    31741 agtttgattg aagtctccca agaaaaagaa attagttctg ataagagtga tctaatgaaa
    31801 gatactcttt acgttacgac aaaatatgat aaagaaatag aggatgcaag atatatggcg
    31861 attcgtgaaa acgagtcgtc tttttcgttg tatcgaatta ctaaagttag cgacccattt
    31921 gaaacattag agtttacagg gttaggattt gcgacaaatg aattagatgc ttacatcatc
    31981 aaagatatta ggccgagtgg gcagccctta aaaaatgttc ttgatcgatt gattgaattt
    32041 actgaaggaa attggcgcgt tggtcacgta gaagcaatgt taccaacagt aactgcaact
    32101 ttttactatg tctctgtaaa agaagcgttg aaagaattgc aaaccttagg tatggaattt
    32161 gtctttaggt gttctttgaa ttctgatgga ataaaggata aatggatcga agtatatgaa
    32221 caaattggcg aagaatcgaa tacacgtttc gtatatggta gtaaagcatt aacagttgta
    32281 agagagatag atagaagctc aatctcaact tcaatgatag gtcgtgggcg aggcgaagag
    32341 gttggtgacg gttacggtag aagaattgaa ttcactgatg ttgaatggaa aaagtcgaat
    32401 ggtgatcctt tagataagcc taaaggccaa aattggcttg aagatccgga atcaactcaa
    32461 aagtatggga taccacaaaa agatggatca atgagaaaac gagaaaccgt agtagtgttt
    32521 gatgatatag atgatccaac agatttactt aaaaatactt attcaacctt aatcgattct
    32581 gctagaccgt tagtacaatt caaagctgaa gtcactggag gagatgtgat aggaaataca
    32641 gtgactattc acagatacga taaaggttat cactataaaa ctcgtattta taaaactaca
    32701 ttcaatcggc ttaccggtca aacgaatatc gaactagggg ataatttaac acaagatgtt
    32761 agaaaacaaa cggcttctat tgtcaataat attaatagtt tagaatctag caaaatgaca
    32821 ttttacgaat cgacagagat tggaaaatat caagatgaca ttatgcgagg tgcaggagat
    32881 aatggcggtt ctatttattg ggtaaatgga attgaagctg gtgttagtga tagtagagaa
    32941 atctatgaaa ctgtttatat ggatggacct aacattccta gatcacgctt ttttatggtc
    33001 caaaataact cagggatatc tttcaaacaa tgtaaaaaag gtgagtggca aacaattcaa
    33061 gatgtacaca atggtgatag cacgaccgct tggacgttgg atggaacttt caatgctaat
    33121 tttattcgcg caggcattct gtctggtatc ctcgtgcaag gggtagcttt aaaaacattt
    33181 gacgataaag atttccaatt agtggcagag gggggagaac tttcttttga aaaacaagtc
    33241 aagtctactg gactagatga tgtacatgga gaacgcttgg gctcaattgt atctacttat
    33301 ggttctaaag ggattaacgg ctttgctgtc tggaaagagc caaactatat tttttccatt
    33361 aatgctgggg acggtggcga tcgagggaat cctatttttc aaattccagc agatgttact
    33421 gctgataagc gcaaatataa tctttatggt gacggcaatt tttcagaagg aaatataacc
    33481 atagatggac ggctagatgt aaaagaactc tatgttaacg gtacaaaaat tgacactaat
    33541 ggaggaggca attccggagg gaacgataac ggttggaacg gacaataccc accagaagta
    33601 actactgatc gggataaacg atattggcaa atttgggcaa cggcaatagg tgccggtttt
    33661 acacaacaag ctgctgcagc cttacttgga aatgcacaag gagaatcaga tgctaatcca
    33721 accgctgatg agggcaatgg cgcaccaggg ttcggttatg gtgtatggca atggaccgat
    33781 tcttctggcg caactagcgg acgtgtctac atgatcaatt taatgacaaa ggctggcatc
    33841 agtgatgatc cagacacaat cacggcacag tttaaattgt tgatgtggca tgcaccaaat
    33901 ggtcaatgga tcgcaactag cgcttatcct tacacatgga cacaattcat gaatctgacc
    33961 gatatcaaca cagcagcaca agcattcgtg gctaactttg aacgtccacg tgatccacat
    34021 ccagaacgga cggcatggtc acaagaatgg tatgacaagt tcaaagattt ggaaattcct
    34081 gtatcaaaag gatatatgaa gccgattgcg gatccaatca cagtgacgag cgaatttggc
    34141 tggcgcactt ctccaatcac aggagcacaa gaatttcata acggtattga ccttgtaaat
    34201 ggaaatccta atacacctat ttttgcttca gcggatggcg aagtgattgt tgcaggtgat
    34261 gcaaattact ttgactggta tggaaattgg acagtgatta aacacgctga tggaatgtat
    34321 acaggatacg cgcatcaaag ccgagtagac gtctcaaaag gtcagaaggt aactgctggt
    34381 cagcaaattg gactgatggg gacaacagga ccatccactg gagaacatct tcattttcaa
    34441 tttatggatg agttttatcc atcttcttca ggtcatttcc acaatgcaag agactacatc
    34501 gatttctaaa ggagggatag tcatggcaga aacgcagcat aaaatggtcc tatccaccac
    34561 cgaaccaaac aacggaataa atttggttcg aattcggcaa ggggatgttt taacgcaaaa
    34621 gttcgttgtt gaagtggtgg aacatggcaa actaaaaaca ttcgagggcc tagtgccgtt
    34681 ttttattaat acaacaaaat ttggcgaaaa ccaacctgtt gaacaaaaag tacaagaata
    34741 cagtccagcg caagcaaggc ttgtttacac cttaagtgag cctgactggc aatggggtgg
    34801 tgaaaacacc gcacatttca gtttccgatc acttaatggt gatggaactt ggagtgaaca
    34861 atttagcaca caggatttta cctatcgagt catttctgga atatctagaa gccagttacg
    34921 tgactctggc tatgtgtgga catttgagga tttgctaaga aaattcaaag attacatgga
    34981 tcagggcaaa aatgactggg agcagtggtt agaagataat cgtgaaatac tggaaaatat
    35041 cgatccaggt ggtatgatca ttaacattct gaatgaagct aaaggcgact acgaatcatt
    35101 agctgatcgt ttaaaccaaa aataccaagt gccagtcggc agctcacaaa ttagagaaac
    35161 aacacgcttt tttgattacg acacgatgaa atacgttgac ctagtgccgc gcaatttgaa
    35221 tacggttgtc aacagtgtta ataacagcaa atttaacttt tctttcatta cagacattca
    35281 cgcagataat cacaacttgc gtatagatgg cgtcggttac aaagatgctt cttatttaag
    35341 acattggcgc gcaatccctc aatttcaaaa attagggaac aaaacagatg tgatgattta
    35401 cggcggagac aatatcgacg gcggacttgg ttcactcggc agcgatatag gcattattga
    35461 tgaatggagc gcgcgacatt ccatgttagg cacgctcaaa cgcttcacta acgcagcggt
    35521 agcaggacaa gaaaaaccgg ttattatctg caaagggaat cacgacgctt gtttcgaccc
    35581 cgcgtggcgc aagcgaaagg gaatgttatg caacgctgac ttcgaagagt attggaacgg
    35641 tttgtacggt ggcgtgttgt tcccagataa aaacgcagca atttaccgtt tcgatacttg
    35701 tgatttttat gaaggcggca caggcgacaa gtacgtggac ggttacagcg atacagcgcc
    35761 gggagctttc agtgccaaac aaattaacgc tttcggagaa tggttagtga acgttccgag
    35821 aaactatcat gtggcgttag taggacatac gcctttaggt ctcagcaagt ttcccgttcg
    35881 caatgaaaac atgattagca cgttgataga agggtttaaa tctggtagtc cggtaactat
    35941 cgattggtct aaattaggac aaccgaatga cggttcgttc ggcggattaa agaccttcgc
    36001 aatgaataca aaaggcgccg gcgttgtagt aggttatttc tgcgggcatt ggcacgaaca
    36061 agtcgaaggg gcgtttggaa cagtaaaaat gattctttgc gacgtcggct tctctcatac
    36121 agctagccaa gttgatacac cggatgaatt agcgttttat aaaatagaag ttgacacagc
    36181 aacaagaaaa gtgacaagca aaggcgtagg gcgcgcgaga gacttcactt ataactatta
    36241 aggcggtgaa caattaaatg ttagattttc aaagcaaacc aaatattttt gaagaaatga
    36301 gttatgaaga ggctgtgaaa tggttattgc gtcaagcggc tatccattac gacggctcgg
    36361 atcacgacgc gcacgtccta gctactgaaa gcaacgcggg ctttgctaca ccggaaacag
    36421 ttatgcaagc gcgtgggcgt tggttacgcg attataagct accacaaaaa tatccgaaca
    36481 ttttagacat tccgcccggc aaatacgcaa ccaaagccgg atggggcgca gacaaccctg
    36541 acgggattga agatgacagt tttgttgaaa tgatggtatt cgcggatcac gattcgagaa
    36601 aattaatcgt cgcttttgct cgttatagcg gcgaaattta catcaaaatg acacataaca
    36661 gcgaaccggt agagggttat aactcgttag gctggcggcg cgtttacact acttctgttc
    36721 tttttgaagg cgaattaaga aaaggacaat cagtcaatct gccagatgat actttccgtt
    36781 accaaacgct tcgtatccac tacacagacg gtgacggaga ctttgtagaa gaagtgaaac
    36841 gtcaaagata cgcgcgaatt acaaaagcga atttatggaa tggagacgcg ggagtgacgt
    36901 tcatcgaatt tgagctgaca atcgaagcgc gaaagataac gatgtcaaac ggcagagctt
    36961 tagatatttc atccggcaat gtttccaatc ccgcaatgag caacaacgtg aaaataacta
    37021 agattgaagg tgtgaaataa tggcgcatgt catcaaaaaa ggctctatca aagtacctac
    37081 acagccgaaa gactacgatt tgcaagcaac ggggcttgta tttaaatcat acgataatca
    37141 aatagcgtta gagttcaacg tcgaacaaca gaacggcaca ccggcggact tgctaggagc
    37201 taacttgcgc ttgttgatgt ttatctatga tgaagtagat ggaatgatca cgaaagagcc
    37261 aatccctttt atcacgaaaa acctcatcac tgaaagcttc ttgaatggac aggtcgtata
    37321 tatcttgcca gaagcgatga aagcttataa cggtatggtg gaagcttatg tttacatcga
    37381 gtatccagac ggatcaacaa gtgataactt aggcttcacc ttccgtatga agcgttcagc
    37441 aatcgacgga ctagcgcaag ataaagcaga ctactttatt gcagactttc aacaattact
    37501 tgatggagtc aaacaagaag caacagatgc tgttaacgca gcattaacaa atgtggattc
    37561 tacgattgaa aaggcaaacc aacaaataaa tgaatttgta gagggagcca cacaagcaat
    37621 tgatcaaact gttgacgagg taacagagca actacaagct actcaaacta agattgatac
    37681 cgtttctcaa aacgttacat cggcacaaaa caatcttaaa gcagttgaag acaagatgaa
    37741 tcaaaccaat cagcaaatcg gcgacctcgg caagctgaaa aagatgtaca gtaacagcat
    37801 cgacttcggg aattatgatt atagtgggaa tccgaattta atgtccaaac taaaatcgag
    37861 cgattttaac gttggttacc acgggtcact aacttcggat aacgaaaagc tacattttac
    37921 ttctgatggt acaggaagca ttattatgtt tacgcgtatt aatacacctc agcttgctag
    37981 tgggaaaacc tatactctga gtgcgaaagt tcgatttgat gaaggaacta caggagctat
    38041 tgataaatta cgtttggtgt atcgtacatc accaggagaa aagatattat tggaagcaaa
    38101 tagtacaaat attacaacag atgatgtagg gaaagaaata acaatcaaag gtacagctaa
    38161 cgttaattat caaatcacaa atttagatcg attttatatg agtattagct ttgttgacag
    38221 ggataaaata aatggcggat ttaagttgta cgacatcaaa atcgaagaag gctcaacagc
    38281 caccccatat cagccaaact tacttgatga cccttactgg ctaggtaaag cgcctttggg
    38341 tgagaatatt gctaataagt ctgttacgtt tccaatcaaa tctagcgcct acagcctata
    38401 ccaagctaac atggaagaag aatttgtttt aggtcaaacc tatacaatta ccatgaaggc
    38461 aaccaaacca cctatccaaa cgttcattgt ttacaacgag gatagtggag attatagata
    38521 tggtaactta gagccagtag aagggttagt tgatacatgg ggtctgactt ttacaccgca
    38581 gaaggttgga gttaactacc ccaaacggtt aactattatc cagtatcccc aatcaacaac
    38641 aggtgcatgt caaattgact ggctcaagat cgaaaaaggc gacacacgaa ccccgaatat
    38701 tagtcagttt aaatactttg gtgaaggctt gaaagacagt aacaatccca atgattacag
    38761 ttgggatgtc acacctgaat atactgaaaa aggcttgaat gatgctgtta atgtgtatga
    38821 tcctcagaga gttgaaggtt tgaaaaactt tgccgatggt attcaaatag caggagataa
    38881 agtgatcagt gaaaatgatt gcactgttta tacattaact aaagacaaca gtcaatcgtt
    38941 tatcgatggg tatgcaacat ttattaaaca cggaaaagaa gttattgtaa atggtacagt
    39001 aaagttcaaa aaagcttatg cttttggtgt accacttgat gatgaagtcc cagatgaatt
    39061 tgtcgcaaaa attgttcatg gcatgcttac aggtccgtca ggaacaagta gtgtatcaaa
    39121 agcgatgtac gtgcgaaaag acttaggaac aattattacg aatagcgaat ttgctgcaaa
    39181 tgaatggttt acattccatg gtaattattg ggtaggggag gaataaaaat gaaaaatatt
    39241 tggaaatacg gacgaacagg tggagagtat gctggacaag tgcttgatga tatggttatg
    39301 actgttccat ttaccgatgt tccaccactc gaaggaattc gtgctgatgg ggaaccacta
    39361 acgattgctg atcagatgtt tgatcctaaa ctgaaccaat ggattgtttt agtgaatgta
    39421 ctagatcaca acgatttaaa caatctcaaa gcgatgtatg agtcgttaga aaatgagaac
    39481 ggcgatttaa aacagctcaa cgccaaactc atgctaagcg atgtagcgat taaacaggaa
    39541 aatactgcat tgaaagaaaa agcggatggt ttagcacaaa tcaattcaaa aacgatgctt
    39601 gcttcgttcc aaaatagcaa agacattgca gaaattaaag agcaactaaa tccagcttca
    39661 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
    39721 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
    39781 aaaaatcatt aataaagata agagcgcatc ttaagtgatg cgtttttatt taaggtaaag
    39841 gagttgtcac atgattaatt taggagaatg gggaacaatc gcaggatcaa tcactgcgat
    39901 tgtttctttg attttattag taataagacc agtcactgca tctttctcga agattactga
    39961 aactctttca aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa
    40021 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
    40081 tacagaaaaa ttggtagaac acacacaaca aatcaaaact ttatttagag aaagatctcg
    40141 gtaaaaaaga aaggagttaa gaagaaatga ttttacccga taagtattat caagtcatta
    40201 aatggacagt tttaacagta ttgccagctg catctgtatt agttgcaaca ctaggcaaag
    40261 cgtatggatg gaatggaaca gatatgacag tactcactat caatgcagta gcaacatttt
    40321 taggcgttat cactggcgtg tcggcttata atttgaaaaa ataggaggaa acaaatgaaa
    40381 aagaaaatta ctattactgc gatgagccta ttaacggctc tttttttatt gccaattaat
    40441 gggtttgcct atacgattaa caatgaattt aatttaggcc caaacgaagg tagctcacaa
    40501 gtagcaaata atcagtacat tttactgcat gaaacggcta atgaaacagc aacaggacgc
    40561 aatgaagcgc agtatatgca acgttcatgg actagcgctt atactgctta tattgtggga
    40621 gacggcggaa ttgtttatca agtcggtcaa cctggttatg tacagtacgg tgctggttcg
    40681 tatgctaatg caaatagtcc tgtgcagatt gagttacaac acacacatga taaagcaacg
    40741 tttgagaaaa actacaaggc atacgttgaa ttagctagag attcagcaat gaaatatggt
    40801 attccactta cactagacac accgtataat caaccaggga tcaaatcgca tttatgggta
    40861 acacaaaata tttggggcga tcatacagat ccttacggtt atctttctga aatgggcgta
    40921 agtaaagaaa aattagccta tgatttggct catggattta ccgatgaaaa tccgacaact
    40981 tcagatgata aaccagtcat tgatccaact cgagcaggtg ctgcaaatcc taccctgaca
    41041 gatggaacaa attacgccca cattgatcag ttcggagaaa tcgaaaacgc aaacttacat
    41101 gtggctggtt ggcacatcgc taactataaa tacgagtata tcttcattat ggattacaat
    41161 actgggaaag aattagctcg agtaaaagct gatggaatat atagaccaga tgtaaatcaa
    41221 gcttataata ctttaggaaa tgttggctat catgtatctt ttaacatgcg caacttccct
    41281 aataagaaag tctatgtaat gatgcgggca acgaatgatc cagaagggaa cactaaaggc
    41341 ggagcacaag attttcatga taaacgctgg tatttaaata ttccgcaacg ataaaaatag
    41401 cccctcgttt ttgaggggaa gtacataatg caaatttgtc cataacttat tgattatctt
    41461 gtaaaaatgc ggtaatttaa tagttagtga gaagaggtgc ttaattatag ttaaaaaaga
    41521 ggaaatagaa gcagcatgta accaagcaaa tgttaggttt attggagaag ctttatctgg
    41581 atatcctaag tataaatact tacacaactg gttacaaagt aaaagttttt atcttaataa
    41641 agaaagatgc atgcctaata aaaattatag ggtttataaa agaggaacta ttgtatatgt
    41701 tgattttggg gtgaacgtgg gttatgagtt atctggtaat cattttgcta ttgttttaaa
    41761 taataaagat aattcgaaaa atggtgttgt atgtgtagta ccaatctctt caaaaaagaa
    41821 aagtaattat gtatctgtag gaaaaatatt agaagttgct agtattaaac aatttataag
    41881 ccaagctgat aaattaaaag agaaacttag gataattatt ttactttcat taaatagagg
    41941 catcattgat gaaagcgatt tgaatagttt tattaaagat attgaagcta aaggtgaaaa
    42001 gctttcagtg gaagaagtag aaaaacgagc tgtaaaacat aatttggttt gcgagacaag
    42061 ggaacaagta attaaagaaa taaaatcatt atctgaagat atggtaaaat atcagaaggt
    42121 gtttaattgc tacaagaaat atacacaaga atcctatgta atgccactta atatccaaac
    42181 gattagtaaa aatagaatta gaagaattaa caagtttgat ccttctggaa atatgaaggc
    42241 acctggaaat gtcctcaatg aagtagatga aagtttaaaa aaatatttta caaagtaaac
    42301 gatatttttg ttgacatttt tctgttcgtt agttaccatg tagttataga aggacgtaag
    42361 cgcctatttt taatgtacac atcgggacgt atgcgcccac atagaaacat agttgagaga
    42421 catctatatg gtgtctcttt ttttattaat atgcaaaaaa gtctagtttt ttcaataata
    42481 tttatgtaat aataaattag ccaccacaac aaagaatgaa acccattatt atctagtcta
    42541 tgtccattct ttttgtttgc agtagttgtg atggctttcc gtactcttag ctcagttggt
    42601 tagagcagac ggctcataac cgtccggtcg taggctcgag tcctacaggg tacatactct
    42661 aagtgattta aattgcgaat aaaatataaa aatgactccc cttttgactc ccccaagcag
    42721 tatatggatg tctctaaaag ttgttttttt agataataaa aagctgataa taagcgaaaa
    42781 acacttaaaa tcagctttaa accctattct cgaaatccgg cgaaccggct aataccggcg
    42841 cgcaggttca aatcctgtac cttccttata tcaacgatta gaggttgtga agaagctgtc
    42901 ctgcatcaag taataagaac ggccaaacaa aaatagcttt tcctattttt tcgtttgatt
    42961 gggcttatta ccgcagatgc aagcttttga acaccgtttg ttcggaataa ggggctgtga
    43021 caagactttt gtcacagccc cttattgttg tacttttaac ctacaatatt ttttgattcc
    43081 ccctgagtac tattttttat aaatgctagt aaatcaatct tctttggcct ttttttgata
    43141 aaaaaaatac tagatgtaag ataaaagtgt aaaaacgatt tgttttagga ggggaaacga
    43201 tgtgtaccgc aatcacttat gcaacgaaag accattattt tggaaggaat tttgattatg
    43261 agatgtctta taatgaagtg gtcactatta ctccaagaaa ttatcgtttc gattttagaa
    43321 aggtaaaaaa tttagataaa cattatgcaa tgataggaat agcagctggg gtatccaact
    43381 atccactcta ttatgaggct acaaatgaaa aagggttgag tatggcagga ctgaattttc
    43441 ctggcaatgc tgattataaa gaattacaag aaggaaaaga taacgttgca ccatttgagt
    43501 ttattccatg gatactaggg caatgctcaa ccatagacga agctaaagaa ttattggcta
    43561 ctatcaatct agtcaatatc gattttagtg aaaaactacc cttgtcacct ttacactggt
    43621 tattagctga taaagaaaaa tcgatagtaa ttgaaagtat gaaagatgga cttcatttat
    43681 atgataatcc tgtcggtgtg ttgacaaata atccgccatt tgattatcaa ttatttaatc
    43741 tgaacaatta tcgctcgcta tcaaatgga
//
LOCUS       DAZKSR010000007        38235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000007 DAZKSR010000000
VERSION     DAZKSR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.007"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8XG_000409"
     CDS             complement(451..1761)
                     /locus_tag="ACG8XG_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0772352.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8XG_000410"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8XG_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0772353.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACG8XG_000411"
     CDS             complement(3431..4999)
                     /locus_tag="ACG8XG_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0772354.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACG8XG_000412"
     CDS             complement(5003..5413)
                     /locus_tag="ACG8XG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0772355.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACG8XG_000413"
     CDS             complement(5410..6333)
                     /locus_tag="ACG8XG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0772356.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACG8XG_000414"
     CDS             complement(6330..7208)
                     /locus_tag="ACG8XG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0772357.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACG8XG_000415"
     CDS             complement(7195..8532)
                     /locus_tag="ACG8XG_000415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0772358.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACG8XG_000416"
     CDS             complement(8535..9638)
                     /locus_tag="ACG8XG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0772359.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACG8XG_000417"
     CDS             complement(9712..11220)
                     /locus_tag="ACG8XG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0772360.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACG8XG_000418"
     CDS             complement(11186..11878)
                     /locus_tag="ACG8XG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0772361.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACG8XG_000419"
     CDS             complement(11900..12874)
                     /locus_tag="ACG8XG_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772362.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACG8XG_000420"
     CDS             13174..14046
                     /locus_tag="ACG8XG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772363.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACG8XG_000421"
     CDS             complement(14504..16702)
                     /locus_tag="ACG8XG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772364.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYTNNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACG8XG_000422"
     CDS             16934..17926
                     /locus_tag="ACG8XG_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772365.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACG8XG_000423"
     CDS             17986..19407
                     /locus_tag="ACG8XG_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0772366.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACG8XG_000424"
     CDS             19400..20899
                     /locus_tag="ACG8XG_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0772367.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG8XG_000425"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG8XG_000425"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0772368.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACG8XG_000426"
     CDS             22491..23174
                     /locus_tag="ACG8XG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0772369.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACG8XG_000427"
     CDS             23176..24012
                     /locus_tag="ACG8XG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0772370.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACG8XG_000428"
     CDS             24023..25777
                     /locus_tag="ACG8XG_000428"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772371.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACG8XG_000429"
     CDS             complement(26053..26394)
                     /locus_tag="ACG8XG_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0772372.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACG8XG_000430"
     CDS             complement(26462..28633)
                     /locus_tag="ACG8XG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0772373.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACG8XG_000431"
     CDS             28844..29701
                     /locus_tag="ACG8XG_000431"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0772374.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACG8XG_000432"
     CDS             complement(29772..30629)
                     /locus_tag="ACG8XG_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0772375.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACG8XG_000433"
     CDS             complement(30614..33316)
                     /locus_tag="ACG8XG_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0772376.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACG8XG_000434"
     CDS             complement(33329..34618)
                     /locus_tag="ACG8XG_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0772377.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACG8XG_000435"
     CDS             34768..35814
                     /locus_tag="ACG8XG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0772378.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACG8XG_000436"
     CDS             35816..37969
                     /locus_tag="ACG8XG_000436"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0772379.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11281 a         7860 c         6802 g        12292 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tgtgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctg
//
LOCUS       DAZKSR010000008        37191 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000008 DAZKSR010000000
VERSION     DAZKSR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.008"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..1105
                     /locus_tag="ACG8XG_000437"
     CDS             140..1105
                     /locus_tag="ACG8XG_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0772380.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1162..1392)
                     /locus_tag="ACG8XG_000438"
     CDS             complement(1162..1392)
                     /locus_tag="ACG8XG_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0772381.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1478..2365)
                     /locus_tag="ACG8XG_000439"
     CDS             complement(1478..2365)
                     /locus_tag="ACG8XG_000439"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0772382.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2454..3677)
                     /locus_tag="ACG8XG_000440"
     CDS             complement(2454..3677)
                     /locus_tag="ACG8XG_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0772383.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3694..4740)
                     /locus_tag="ACG8XG_000441"
     CDS             complement(3694..4740)
                     /locus_tag="ACG8XG_000441"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0772384.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4904..6631)
                     /gene="ptsP"
                     /locus_tag="ACG8XG_000442"
     CDS             complement(4904..6631)
                     /gene="ptsP"
                     /locus_tag="ACG8XG_000442"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0772385.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6631..6897)
                     /locus_tag="ACG8XG_000443"
     CDS             complement(6631..6897)
                     /locus_tag="ACG8XG_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0772386.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7376..9610
                     /locus_tag="ACG8XG_000444"
     CDS             7376..9610
                     /locus_tag="ACG8XG_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0772387.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9758..10078
                     /locus_tag="ACG8XG_000445"
     CDS             9758..10078
                     /locus_tag="ACG8XG_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0772388.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10283..10507)
                     /locus_tag="ACG8XG_000446"
     CDS             complement(10283..10507)
                     /locus_tag="ACG8XG_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0772389.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10705..12285)
                     /locus_tag="ACG8XG_000447"
     CDS             complement(10705..12285)
                     /locus_tag="ACG8XG_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0772390.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12686..14062)
                     /locus_tag="ACG8XG_000448"
     CDS             complement(12686..14062)
                     /locus_tag="ACG8XG_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0772391.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14190..15278)
                     /locus_tag="ACG8XG_000449"
     CDS             complement(14190..15278)
                     /locus_tag="ACG8XG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0772392.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15450..15884
                     /locus_tag="ACG8XG_000450"
     CDS             15450..15884
                     /locus_tag="ACG8XG_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772393.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15962..16495)
                     /locus_tag="ACG8XG_000451"
     CDS             complement(15962..16495)
                     /locus_tag="ACG8XG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0772394.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16589..17767)
                     /gene="mutY"
                     /locus_tag="ACG8XG_000452"
     CDS             complement(16589..17767)
                     /gene="mutY"
                     /locus_tag="ACG8XG_000452"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0772395.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17760..18557)
                     /gene="recX"
                     /locus_tag="ACG8XG_000453"
     CDS             complement(17760..18557)
                     /gene="recX"
                     /locus_tag="ACG8XG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0772396.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18664..20046
                     /gene="rlmD"
                     /locus_tag="ACG8XG_000454"
     CDS             18664..20046
                     /gene="rlmD"
                     /locus_tag="ACG8XG_000454"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0772397.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20220..20798)
                     /locus_tag="ACG8XG_000455"
     CDS             complement(20220..20798)
                     /locus_tag="ACG8XG_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0772398.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21120..21590
                     /locus_tag="ACG8XG_000456"
     CDS             21120..21590
                     /locus_tag="ACG8XG_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0772399.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21698..22924)
                     /locus_tag="ACG8XG_000457"
     CDS             complement(21698..22924)
                     /locus_tag="ACG8XG_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0772400.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23678..23878)
                     /locus_tag="ACG8XG_000458"
     CDS             complement(23678..23878)
                     /locus_tag="ACG8XG_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0772401.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(24012..24620)
                     /locus_tag="ACG8XG_000459"
     CDS             complement(24012..24620)
                     /locus_tag="ACG8XG_000459"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0772402.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24705..25175)
                     /locus_tag="ACG8XG_000460"
     CDS             complement(24705..25175)
                     /locus_tag="ACG8XG_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0772403.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25223..26137)
                     /locus_tag="ACG8XG_000461"
     CDS             complement(25223..26137)
                     /locus_tag="ACG8XG_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0772404.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26283..27086
                     /locus_tag="ACG8XG_000462"
     CDS             26283..27086
                     /locus_tag="ACG8XG_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0772405.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27264..28211
                     /locus_tag="ACG8XG_000463"
     CDS             27264..28211
                     /locus_tag="ACG8XG_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0772406.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28279..28572)
                     /locus_tag="ACG8XG_000464"
     CDS             complement(28279..28572)
                     /locus_tag="ACG8XG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0772407.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28600..29265)
                     /locus_tag="ACG8XG_000465"
     CDS             complement(28600..29265)
                     /locus_tag="ACG8XG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0772408.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29405..30037)
                     /locus_tag="ACG8XG_000466"
     CDS             complement(29405..30037)
                     /locus_tag="ACG8XG_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0772409.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30044..31111)
                     /gene="liaS"
                     /locus_tag="ACG8XG_000467"
     CDS             complement(30044..31111)
                     /gene="liaS"
                     /locus_tag="ACG8XG_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0772410.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31108..31839)
                     /gene="liaF"
                     /locus_tag="ACG8XG_000468"
     CDS             complement(31108..31839)
                     /gene="liaF"
                     /locus_tag="ACG8XG_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0772411.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(32008..32487)
                     /gene="greA"
                     /locus_tag="ACG8XG_000469"
     CDS             complement(32008..32487)
                     /gene="greA"
                     /locus_tag="ACG8XG_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0772412.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32623..33798)
                     /gene="mltG"
                     /locus_tag="ACG8XG_000470"
     CDS             complement(32623..33798)
                     /gene="mltG"
                     /locus_tag="ACG8XG_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0772413.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            34002..35171
                     /locus_tag="ACG8XG_000471"
     CDS             34002..35171
                     /locus_tag="ACG8XG_000471"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0772414.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(35438..37072)
                     /locus_tag="ACG8XG_000472"
     CDS             complement(35438..37072)
                     /locus_tag="ACG8XG_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0772415.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        11358 a         7373 c         6081 g        12379 t
ORIGIN      
        1 ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc tgtgaacaga
       61 actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc tttttattca
      121 aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca tgctgccggg
      181 tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag caaaacggct
      241 caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa aagtattacg
      301 tatgattcat catataacag attttgattt cgtcgtagta gacacggaat tagatgcttt
      361 attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac aaatgaatta
      421 tttcagtaat tataattatg tacatattac caataaggga tttgtattaa cagatactcc
      481 tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat
      541 aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag ctttgcgagt
      601 ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac tccaagggct
      661 gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac aacatttcat
      721 ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat tagttactta
      781 ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc tcaccttcag
      841 catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt atggtcaact
      901 tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg aaaagaaaga
      961 agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt
     1021 gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt taaacaaaaa
     1081 agaagctgaa aagcaactaa attagttgct tctcggcttc ttttaggcat cttcaacttc
     1141 aaatgtcaat tatcctaaaa tttattggat taagtcatag atttccatcg ctacgatatc
     1201 gatattatca aattcgtatt gctgatgagt atctgtttca gttaattcaa atgtatcggt
     1261 cgctttatca taagttactg tacacttctc tacgccttct ttttcaaaac ggcgaacttg
     1321 cagttcatta tctgctgact cgaccatagc ctccagtcgt tttatgattg cgactaattg
     1381 tgaatgtgtc atcggaggtc ctccttttct tatttctgtt atattgtatc aaaagcatag
     1441 cctacttgca ttgattttct ctaaaaaaac tcgtttttta tgattttttt tctggtattt
     1501 ttccttcagg ccaccaaagt ttgatcaaag cttgggttcc gtcattctca aaacctttat
     1561 ctgccaactc ttcatatagt ttcaatgctt gggtagttgc tggcaataca agatccattt
     1621 tttctgcttc ttctaatgca atctttaaat ctttgataaa atgtttgaca aaaaaaccag
     1681 gtgtgtaatc ttcttttaaa atacgaggag aataattgct taaagaccag ttagctgcac
     1741 ttccacctga aagcgtctca atcactttct ttaaatcaag tccgtttttt tgtgcataga
     1801 caagcatttc cgtcattcct gtcatcgtac ctgcaatcat gatctgatta gccatttttg
     1861 tatgctgacc tttcccagca gaaccatgca atgtaaatgt cgtaccaaat tctttgaata
     1921 aaggcagtac ttgatcataa acggcttctt ctccaccgac catgatcgtc agtgtaccat
     1981 tctttgctcc taagtcacca ccggataccg gtgcatctaa cgcatctgcg cctttttctt
     2041 ttgctgtttt tgcaattttt tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa
     2101 tatgtcctga gatatctgca gaaaaaatcc ccgactgtcc atagtagatt tcttctacat
     2161 ctttggggta acctaccatt gtaaagagaa tattactttg ttctgctacc gcttttggtg
     2221 tatctgccca gatagctcct tctgttacta aaggatcagc ttttgctttt gtccgattat
     2281 agacagctac ttcgtgtcct gcttctagta gatggcgtgc gacagcactt cccatgacgc
     2341 ctgttccgat aaaaccaagt ttcatttttt cgacctccta aaaatcaaaa atccttgctt
     2401 ctatgctagt atacgctaga aacaaggatt ttttcataaa aaatgtttga attttatctt
     2461 ttaaatgaat ttaattttat tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc
     2521 atctcttctt catgataaac aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa
     2581 tgtgtagatg aaatatcata aagtttagct tctaaaactt ttggatcttt aggaacttca
     2641 ctggaaatat aatcaataag tgctggtgca aagtcttcgt cggtttcaaa agttttccct
     2701 aatgaaggat gatcgaacaa tcggttcaaa taagcatttc cttccacgac acaaggtgtt
     2761 ccggctgcca tcgcttctgt ataagtaagc ccttgtgttt ctgaagtaga agcactgaca
     2821 aaataatctg ccgctttata ataaagtgct acatcattgt tgtccacttc tcctgtgaac
     2881 tgcacaaatt cttctaactg cagttctgag atcaattcct ctaactcttc tacataaggt
     2941 ccttttccta cgataactaa tctggcgtta ggataagcgg agatgacctg aggaaaccca
     3001 ttaatcaagg cttggatatt tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa
     3061 tggttttcct gaagacctag tgactccctt aatttgctga tatcctgctg agtgatatca
     3121 gaacgtttga atttttcaat gtcgatacca gtaggaatca cgcgaagcgg tgctattaca
     3181 ccatagctgc gtaatgtatc aataacacgt tcacttggac aaactacacc tgtcgtatga
     3241 tttgcaaata atctagacaa gtattttaca tgggattgcc tgatcacttt tcctttaccg
     3301 atataatgaa ggtagtcttc atacatcgta tggtaagtat ggatcagagg tattttcaac
     3361 ttcttagcta ccatcttccc aagtatccct gctccgaatt ctgtatgggt atgaataaga
     3421 tctagttcta gttctttggc aaccaaataa gcataccaca tgcctctaac aacaactctt
     3481 cgatccttga aagaaataaa tggtacactt ggcattcgaa tcacatcttt ttccaactta
     3541 tccgcattag gatcggtcgt tgtaaaaata tagacttcat gcccatgtct ttctaattct
     3601 tgttttaatg ttttgatcga tgtagcaact ccgctgactt gcggaaagta ggtatcggta
     3661 aaaaagccga ttttcatgtt tttcgcctcc aaactagtcc ttgacctgat ttactttttt
     3721 taatttgcta gagacttttt cattcagtac actttcgtat acatttttaa gttcttcacc
     3781 gattttattg atacttcgct cttttgctgt ttgataaccg gcttgtgtcg tactcggtag
     3841 ttcttgattg gtaatctgtt ggatcagtcg gataaattct tcgtttgtct tccccatata
     3901 gcagttcttg ctatcttcta accagccatt atacactgga atatctcgaa caagtacgtt
     3961 ttgtttgctt gctaaagctt ctaaaacaac gatcccttct gtctcttcat aagaagggaa
     4021 aaagaataga tctgcacctg aatacgcacc ttctatgatc tctccacgaa tgtaaccagg
     4081 aaacaggaca ttttcaggat gatcttcttt tacaattttc cggatgttcc gaggaatcga
     4141 gtacatggga acatgaccaa accaaataaa tgtgtattcg ggcatcttct tagcaatttc
     4201 tacgaaatca atgattcctt tacgttcgaa aaacaaaccg acacaaatga ttactttttg
     4261 atccggagac agtttgaaat attctctgaa cttttgttct ttcattggat cagggtgata
     4321 tttctctaag tcgatcccat tggaaatagc ttgaatcggc acacggatac catagctttc
     4381 caacaactgt tttgaataag gtgttggcgt gatcagatag tccgcttttt ggtacaatcc
     4441 taccagatat ttttttacga taggagaaag ctggttcgat ctgataaagg aatttcggaa
     4501 atcttcctct gtagaatgcg catgatagac aacttttttt ccttctcttc tggctttatt
     4561 gaccatatta tgactattga ttccgtatgt attgatgtgc aggatgtcat aatcttcttt
     4621 ttcatctagt gtataagaga taccgacttc cgtcagagca cgtttttgat gatcaagcgc
     4681 acgcccgatt ccagattttg caagcatctt ttcactttca aaatataata ataccttcac
     4741 cttacgtccc ccttcactta tatagtataa catagcctac aaactgtcac tcaaacttaa
     4801 gccctatttt tgtctattct tactgacaaa aacgttttct taaaaaagaa caaaaaaact
     4861 ggcgtagctc ttttacaagc cacaccagct tttgatacgt tgtttatttt acatattcat
     4921 gaacaagttc aacgacttct tccatcatat cgcattcttt caatgcacga tcagcaagtt
     4981 cagccatttt cgctgtgtcc aagcgtttca ttaaactacg cgttttaaga atagatgtcg
     5041 cactcattga gaactcatct agacccatac ctactagcaa tggaacagct gtttgatctc
     5101 cagccatttc tccacacata cctgcccatt ttccttcagc gtgtgctgca tcaattacat
     5161 ttttgatcaa acgtaaaatt gatgggttat atggttggta caagtaagaa acgcgttcgt
     5221 tcatacggtc tgctgccatt gtgtattgaa tcaaatcgtt cgtacctaca gagaagaaat
     5281 ctacttcttt tgcaaacttg tctgctaaga ctgctgctgc tggaatctcg atcatgatgc
     5341 cgacttggat cgtatcagat acttccttgc cttcacttat caatttttgt ttttcttctt
     5401 caaagatcgc ttttgctgcg cggaattctt tgagtgtagc taccataggg aacatgatac
     5461 gtaaattacc atgaacagat gcacgaagca atgcacgcat ttgtgtacgg aacatttcgt
     5521 cgccttgttc agataaactg atacgcaatg cacgatatcc taagaatgga ttcatttcat
     5581 gaggtaattg cagatatggt agttctttat ctccaccgat gtccattgta cgaacaacta
     5641 caggttttcc ttccatacct tctaatacag ctttgtaagc ttcatattgg tcatcttctg
     5701 ttgggaagtc tggagaatcc atatacagga attcagtacg gtacagacca actgcttctc
     5761 caccattatt atggacacca actagatctt ttggtgttcc gatgtttgca gctaattcaa
     5821 aatgcttgcc gtcagctgtg acagtttctg cattttttaa tttttcccat tctgcttttt
     5881 gtgctgcgta atctgcacct gctttttcaa attcagcttt ttgttcatct gtaggatcga
     5941 tgattacatc gccttcgatc ccgttgacag ccaaaatatc gccttctttg actttagcag
     6001 taatttcttt tgtcccaacg attgctggaa tttctaaaga acgtgccatg atagcagaat
     6061 gtgaagtacg tccaccaatg tctgttacaa acgcttttac aaaattgcga tctaattgtg
     6121 ctgtatcact tggagtcaag tcatgtgcaa caacgaccac ttcttcgttg atcattgatg
     6181 gatttggtaa agtaacgcct aataagtgcg ctaaaatacg tttagcaacg tcacgaatat
     6241 ctgcagcacg ctcttgcata taagcattat cttccattgc ttcgaacata ccgatataca
     6301 tatccgttac ttcttttagc gctgattcag catttacgct gttgtcttta atattttgct
     6361 tgatttgacc taccatttct ggatctgaaa gaaccatcaa atgggcatca aatacttgcg
     6421 cttctgcttc acctaagctt tgcgctgctt tttcacgaat ttgctgcaat tcttcagtag
     6481 atttagctaa tgcagcatcc aaacgagctt cttcagccgc agtatcttct actgttgcct
     6541 tactgaatgt taaatccggt tgaactagca ggtaagcttt tgcaacggct acaccgtcac
     6601 tagcggcaat cccttttagc atttcaacca ttattcagat aagccttctt tcttcattgt
     6661 ttcaacgatt gctgccattg catcagcttc gtcagcacct tctgcagtaa tagtaacatc
     6721 agaaccttga cctacaccta aagacataac gcccatgatt gatttcaagt ttactgattt
     6781 acctttatac tctaagttta catcagagtt aaatttgctt gctgtttgta ctaatagtgt
     6841 agctggacga gcgtggatcc cagtttctgc tactacgtga aattctttct tttccatatt
     6901 aatcggtctc ctttaagtga gtgttgtttt tatgtgaggg ttttcttttt tagtaattca
     6961 gagaatccta acaaatgcca ccctttcatc tgtaaagatt aacatttttt ttcgttagat
     7021 acaacagttt tcattcaatt tcagataaaa gtattcaaat tattccggat tctttttcat
     7081 ccttttcttc acattttata acaaacgtgt ttatttcctt tttcttgtcg ctttctcttc
     7141 tcctgacgtt tatcattctt tcctttgacc ctctttgttt tcatatgaaa cgaattactt
     7201 gcttcccttt ttatttttag tataataaga gtcaaaggtc aggaatggtc aatagagatt
     7261 tcaccttttc tacctcataa atctcttaaa aaactagggg ttattctttt acctcatgaa
     7321 aaacgagatg taaaatatgg atgtacccat ttttgtgaaa ggacgtgaat gattcatgct
     7381 ttgtcaaaac tgcggcaaga atgaggcaac gattcacttg tatgccactg tcaatggtca
     7441 gcggacacaa ctcgattact gtcagagctg ttatcaaaaa atcaaaaatc aacaaaatgg
     7501 aggtctgact aatatggccc aaaatgatcc atttggattt ggcagtttgg acgatctttt
     7561 ccgttccatg tctcgccaaa tgcaacagca aggaaattat gagcaaacac caccaacaca
     7621 atttggcgga aacaacaact tcaatggtgg tcagccacct caaggcaata cagaaggttt
     7681 attgggagaa tacggaatca atatcacaga acaagcacga caaggtgata tcgatcctgt
     7741 gatcggacgc gatgacgaaa tcaaacgagt cattgaaatt ttaaatcgcc gaaccaagaa
     7801 caatcctgtc ttgattggag aaccaggtgt tggtaaaaca gcagttgttg aaggtttagc
     7861 acaaaaaatc gtagatggtg atgtaccgca aaaattaatg gacaaagaag tcattcgttt
     7921 agatgtcgta tctttagtcc aaggtactgg tatacgagga caatttgaag aacgtatgca
     7981 aaaattgatt gaagagatcc gtcaagcaga aaatgtgatc ctattcatag atgaagttca
     8041 cgaaatcgtc ggtgctggtt ctgctggtga tggaaatatg gatgctggta atattttaaa
     8101 accagcttta gctcgaggag aattgcaaat ggttggtgct actaccttaa atgagtaccg
     8161 catcatcgaa aaagacgctg ctttagaacg tcgtatgcaa cctgtacggg tagatgaacc
     8221 aactgtcgaa gaaacgatca gtattttgaa aggtttgcaa aaacgttatg aagactatca
     8281 tcatgtaaaa tatactgatg aggccatcga agcagccgca actttatcca atcgttacgt
     8341 ccaagatcgc ttcttgccgg ataaagccat cgatttgcta gatgaaacag gttctaagaa
     8401 aaacttgacg atccaaatcg ttgatcctaa aacaatcgag aaaaaactgc aagaagcaga
     8461 agaacaaaaa gttttagctt ctcgagaaga agatttcgaa aaagcagctt attatcgcga
     8521 tcagatcaat aaacttcaaa aaatgaaaga acgtcagctc actgaagagg aaacacctgt
     8581 gattaccgaa aaagatatgg aaaaaatcgt tgaacaacgg acaggtattc cagtgggtga
     8641 attaaaagaa aaagaacaga cacaactgaa aaacttagca gatgacttga aagctcatgt
     8701 catcggacaa gataacgctg tagatcgagt agcgaaagcg attcgccgaa accgtgttgg
     8761 attaaacaaa cagaatcgtc caatcggctc cttcttattt gttggaccaa ctggtgtagg
     8821 taaaacagag cttgcgaaac aacttgccta cgaactattc ggttcacaag attcaatgat
     8881 tcgtttcgat atgtctgaat atatggaaaa acatagtgtc tctaaattga ttggttctcc
     8941 tccaggttat gtcggatacg aagaagctgg tcaattaaca gaaaaagtac ggagaaatcc
     9001 ttacagtctt gtcttgttag atgaagtaga aaaagctcat ccagatgttt tgcatatgtt
     9061 cttgcaaatc ttagatgatg gtcgattgac agatgcacaa ggaagaacgg tcagctttaa
     9121 agatacgatc atcattatga caagtaatgc cggaactggt aaagtagaag caaacgttgg
     9181 ttttggtgcg gcacgcgaag gtgttactcg ttctgtattg aaccaattaa acaactactt
     9241 tacaccagaa tttctgaacc gttttgacgg gatcatcgaa ttcagtgcat tatcaaaaga
     9301 aaacttgatg acaatcgtca cactaatgct agacgatgtc aaccaaatgc tggcagcaca
     9361 gcaacttcac atcgaagttc ctacgaatgt caaagaaaaa ttagttgact taggctatga
     9421 tccatctatg ggtgctcgtc ctcttcgacg cacgatccaa gaacaaatcg aagatggtat
     9481 cgctgaattt tatcttgatc acccttctat ccatgagttg aaagcaaaac tggataaaga
     9541 tggaaaaatc atcgttactt ctaaaccaga acgtttggct aaagaatcag ccgaagaaac
     9601 ggctgaataa atccagtttt atagcgtgag acggtatttc tttgagaaat gccgtctcat
     9661 tcttttttca gaaaagtcaa aatactgttc attttataaa aaagaaatta taataagaga
     9721 aacaagcaat taaaaataca taaaagaggt gagcgccatg aaattggtaa atgtaacaaa
     9781 cagccattcc cgtcttgtat taaaccagct tgaaaacact gatgcccatc tagtcaaggt
     9841 ctacacagcc ggaaatacca ctattgtcta cactgaagcc cctgaacata atgaaattct
     9901 gctgatcaat gacaaacgta aaatccagcc gaaagaaatc gaagatgcta aaaattattt
     9961 cttagataaa atcaaagacg ccgtttacca cgaggatgaa atcaaagtaa tcgaattggc
    10021 aggccttgtt gaaatctcga ttccaaaaca tgaattaggc gctacattag ccgtgtaaac
    10081 aaattttgaa cataataatt aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
    10141 gctctaaaca cgtataaacg gcgaaagcag aagcaactcc ttcggaaata agctgaaatt
    10201 cacgaaaatt gcttttcaaa tttttgtgaa ttccagctta tttcttggag ttaaacgctt
    10261 ctgtctcagc ctctttttct ttctagcttt ttgatttttc tcttttcttt tgtacttttc
    10321 tcttttcatt ttcagttcta cgcgtgtctt tatttttaac tttttcctct ttatggatga
    10381 ttgatcgtac tttttgtgca atttgctcgt ttttgtccca gtcttttaca tctaataatc
    10441 gacataatcg taacatctct tcttttttct gtttttttct ttcacgtctt tgccattctg
    10501 tttccatgtg tcacactctc ctttctttaa tcatatgtac gcaaaataat ttttatattt
    10561 ttttgattag acatttctat gattttaata tagtaaaaag agccgtcacg gaaaatccgt
    10621 cacggctcta aacaaaatat gagaggctgt ttttgtttgg ctattcttat tacttgatgc
    10681 agggcagctt gtgtcaagac ttctttacat caaacttttt aattttacat tgggatattt
    10741 atccgcaaac cagcgttctg caaattggtt ctcgaataag aacaacggtt ggtcgaaacg
    10801 gtcacgagcc aagatattgc gacttgaact catcttctca tccaaatctt ctggatcgat
    10861 ccagcgagcg attttgtgtc ccattggaga catgattact tcggcgttat actcatttaa
    10921 catacgatat tggaatactt cgaattgtag ctgaccaaca gctccaatga tatattcttc
    10981 cgttagatat gttttataaa gctgaatcgc tccttcttga actaattgat agattccttt
    11041 gtggaaggat ttttgtttca tgacattttt tgcagtcact ttcataaaca gctctggagt
    11101 aaatgaaggg agttcttcat aagcaacttt tagttttcct tcgaacaatg tatccccaat
    11161 ctgataattg cctgtatcat atacaccgat gatatcgcct gctactgctt tttcgacgtt
    11221 ttctctcgaa tctgccataa attgtgtgac attgcttaat ttcagttttt tatttgtacg
    11281 ttccaaccag acatccattc ctcgttcgaa tgttccagaa cagattcgga caaaagcgat
    11341 tcggtcacga tgggctggat tcatatttgc ttggatcttg aatacgaaac ctgaaaattc
    11401 ttcctcataa ggacttacca tctgatcatc ttgtgttttg tgtccataag gagctggtgc
    11461 caattcaacg aaagtctcta aaaaagtctg tacaccgaag ttagtcaatg cagaaccaaa
    11521 aaatactggt gtctgatcgc cacgagcaat tttttcttga ttaaatgcat ctccagcctc
    11581 tttgattagt tctacttctt ctaagacttg ttcatataag gaattttcag ttagaggatg
    11641 attttcggga agcagtccat cttcatccaa ttctactaat cgttcatcct gataattttc
    11701 cggacgataa aactctacac gattatggta gatatcataa agaccttcta gccctttacc
    11761 cataccgatt ggccaattca tcggataaga ttcgatatct aatagctctt ctaattcttc
    11821 taacaattcc aatggttcac gaccatcacg atctagtttg ttgataaaag taaatatcgg
    11881 gatgccacgt ttttttacaa cttggaataa tttctttgtc tgtgcttcga tcccttttgc
    11941 actatcgatt accatgaccg cactatccac ggccatcaaa gtacgataag tatcttcaga
    12001 aaaatcttca tgtcctggtg tatccaagat atttactcgt ttcccttgat aatcaaactg
    12061 cataacagaa ctagtaacag aaatcccacg ttgtttctcg atttccatcc agtctgattt
    12121 ggcaaaatta cctgtttttt tgccttttac ggtcccggct tgacggatag ccccaccaaa
    12181 caatagtaat tgttctgtaa tcgttgtttt cccagcatcc ggatgggaaa tgatcgcaaa
    12241 agttctgcga ttgtccactt gttgctttaa ttttggatta ttcataacct aactccattt
    12301 cttccattca attatttata ttttctctag gcacagattg tccgatctct taaagaaaga
    12361 caatttcata tagggctgtg acaaaagccg tgtagcagtc tctttttccg agtaaacggc
    12421 gttctagaga cagcctatca gcaataagcc cacccaagcc aaaaatacga aaagctgttt
    12481 ttgcttggct gctcttatta cttaatgcag gatagctttt tcacaacctt tgtcaactac
    12541 tacaactgaa tcagtatagc atggattcag cgaatgaaaa agccaccaaa ccataagagt
    12601 caaatatttt tttgtcagct gactaaaaga aaagacgtgt tgcttgttta ttcaagcaac
    12661 acgtctttca taaccaagtt tccaattatc ttcttggctc gtcatcttcg aattcttttc
    12721 tgaagtatct tcgttcttct tcaggttccg cttctgtttc ttccggatca tgaaaaatga
    12781 cacggatttt aaggattcga gaaccttcca tttcttcaga aaccagcgta atattatcga
    12841 catcaaacga taacttttcg ccttcatccg gaatcatgcc taatgctgta atcaaataac
    12901 cagccatcgt atctacatca ctcatatgca gatcactgtc aaaagcttca ttgaactcat
    12961 caatcagcat acgtccttgg atgatatatt cgtgttcatc gattttttca taaagattct
    13021 caacttcatc tgattcatca tcgatttctc cgacgatttc ttctaaaagg tcttctagtg
    13081 ttgctaaccc aacgacgccg ccatattcat ctaataaaat agccatctga ttctgcgtct
    13141 ttttcatttc atataacaga tcatcaataa agaccgtttc aggaacgaat aaaggctcct
    13201 gcatgatttt cttgatatct aaattatcaa agccgaattt atgggcagct tttagtaaat
    13261 tctttgtatg aaggattccg actactttat ctttgtcttc gttataaaca gggattcttg
    13321 aatagttttc ggataaaact tcattcacat tttcttccac agtgtcgttg atatcaatca
    13381 taaatgcatc cgttcgtggg accatgactt ctcgggcaac tttcgtatca agagaaaaaa
    13441 ccccttggag catttctaat tcttcattat tcaatacgcc ttcgttttca agcatataac
    13501 gcatttcatc tctggtcatt tttgaatctt catcatcaaa agtcatcggc gtgatacgcg
    13561 ccaataaact tgtggaagca gaaagcagcc agacgaaggg cttagctact acaccaatca
    13621 agcgtatcag ccctgaagtg aactttgcga cttcttctga tttatttaaa gcaatacgtt
    13681 tagggtacaa ttctccaaaa acaatcgata cataggtcaa aatagccagc acaatgatat
    13741 ttgcaatatt ttttgccgct gtgccgccac ctaataccgg agctaaccgt tccgataaag
    13801 tattagctag tgaagcacca gacaaaatgt tgaccaaagt gattcctact tggattgtcg
    13861 ataggaaatt atttggattc tgcaatattt ttaaaagctt ttgagatttc acatctcctt
    13921 catcagcttt ttgctcgatt cgattcttat tgatcgaaac aacagcaatt tcggaagctg
    13981 ctaaaaaagc attgatcaat gtcaaaacga tcaataataa aatctgcgct aacagcgact
    14041 gactctcagg gtcagcattc ataatcgcat tctctcctta taaaattaat ttttagttta
    14101 cgatagaaaa aaacgtggca ctatagccac gttaaaatgg tatcacaaag caattttctt
    14161 tgcaataaaa acaagtgtac taaaactacc tacgaggtcg tagcaatttt cttcttgttt
    14221 tcattttgct tgtcttcttt gacgattttg attatatagg aaatgatcgt cttgcagatt
    14281 gcgtaaaaag gaacacctaa gaagatacca agcaatccgg caatatttcc agctactaga
    14341 agaacgataa tgatcgttaa tggatggatt ttcagtgatt tcccaattac attaggataa
    14401 atgatattcc catccaactg ttggacgatt agcacaacca cacaacaaag taatgctttt
    14461 accggatcat tgaagactgt tactagaaat gcaggtgcta gtcctagata aggtcctaaa
    14521 tatggaatta ggtttgtcaa tcccgcaatg accccaaata agaaagcata atccacacca
    14581 attaccatat atcctaaaaa tgtgaaggta ccaacgaata aacattcgat tgcttgtccg
    14641 ctaatgtaat tagccaatgt tttattcagt tgccccaata actccacaat ttggtttctt
    14701 cttttttctg ggaaaaactg ttggatgttt ggcactagtt tttctccgtc tttcaacata
    14761 tagaacaaga tgaatggtgc tgtaatgatt actacggttg ccgaagcgat cgttccaacg
    14821 atcgaaccta aactattgga tactccacta aggaattgct gaatgattgt cccgtaagaa
    14881 atgtctaatt gctcaaaata tttattgata tctacttcct tgaacaaagg caattcggct
    14941 acctctttta accagctttc tacctgtttg atgaaatcag gcatattaga tgccaatgag
    15001 gaaatttgct gtactagact tgggatgaca ctaagtaaaa tccaaataat tgctgcaact
    15061 aaaaggagca gcacaattgc gacagcccat attcttttga tttttgcttt catcaacagc
    15121 ttgacaatag gattcaataa atagtaaaga aagcctgcaa tcagtacagg tgcaaataac
    15181 gtggaaaaaa atgtcccgat cggttcgaaa ataaagttta tttctgatga aatcatgatc
    15241 aatgcagcta tgatcaataa ttcaagcgac caaaacatta gtttagactg tctaagtttt
    15301 tcaaacaata gatttcctcc tttctttctt actagtatat attatattcg ttaaaaattc
    15361 ttttattctt tttttatttt tgaaaacttg ccatactttt tatttgtttt attttccagt
    15421 atactatacg aaaggaaggt ggctagacca tgaaagttgt acaaacaaaa gatacaatga
    15481 gcaatattta cttagatgct gtacggatca ggcatcaagt ttttgtagtc gaacagggtg
    15541 tgcctttgtc tcgagaaata gacaaagatg aagcccattg tatccacttc gtcctgtact
    15601 cagataaaaa agaaccgcaa ggaactgtac gtcttttgcc tttagaaaac ggaaaaatga
    15661 aattacagcg gatggcgatt ctttctgaat acagacacca agggttagga aaaatattga
    15721 ttgaagaagc agaaaacttt gctaagaatc aaggctataa tacgatttta ttaggggcac
    15781 agtccacagc tgaaactttt tacgaaaaac taggatatac agcttacggc gatccttttg
    15841 aagatgctgg catgcctcat atttatatga aaaagacttt ataaaaattt atttcgaaaa
    15901 aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg gcttactgtt cttttttgtg
    15961 tttacttacg cgacaattgc ttgtaacggt tataccaaat atcaatatag ccttccgaga
    16021 acgggccatc tccattattg atccagtcaa ccagaatctt cacattttct tttaggatga
    16081 agtcaatatc gttcgggtaa ttcatcattt tgctgtgcat ctcatattca tcgacatcca
    16141 atagtcgttt ctcgccgtca ggaaatacct tgatatccaa gtcatagtcg atatatttca
    16201 aggcctcatc gtccagcaaa aaaggtgaag ctagattaca ataataggaa accccctttt
    16261 ctctgatcat tgctattaca ttgaaccagt attttttatg aaaataaaca atcgctggtt
    16321 cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa tgtgtgatca ttcacgccga
    16381 tcatagagtg ttcgcttgtt tttaatacca tggtatctcg ccacgttcga tgcaatttgc
    16441 cgtcgtgctt gtaactttgg atcgtcacaa actctccttc ttttggaact cccatagcaa
    16501 cccttcctta agcagcattt tgtgctttgt gtttgggtgt tcattacacc gacatcataa
    16561 ttattatatc aataaaaatg agctttgcct agaaaggatt cctattgttt cgatttttct
    16621 ctaactgatc taccagtttc atttggacct taggaaaaac gaccgaatca aatgcatcag
    16681 gcttcaacca cttcgaagtt ttgttctcgg taaattcttg ttctgctccc tcggttgccc
    16741 gtccgtaaaa aaggagaaca tgccatttca gatgactaaa aatatgagta acttctccta
    16801 aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa tgggagattt tgctcttctt
    16861 ctgctactag gtcatcaaac agatcgattt ctggctttgt ttcccattct tttttcaatc
    16921 gctcatactc ctcttgtgtc acttccatca ttggaaatgt ccacatattc gccagcaatt
    16981 tttggctatc tctttcttca aaataatacg ctccagaatg attttgaaga gctgcactga
    17041 tataataaac gtcttttggc tttgcttttt ttgtttttac aggaaaactc gtttggattc
    17101 ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt ctcacacttt ggagaagttg
    17161 gtgtacagat agctgaacct agatccatca ttgcttggtt gaattcacct ggatgctttt
    17221 catcaatgat tttccgcatc gcttcatcaa agattttcct gctagaagct tttgcgatgt
    17281 ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac gttgccgtca actgcaggtt
    17341 ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt atatggtcct attcccttca
    17401 atgagctaat ttcttcaggc gtttgcggca tttccccatc aaattctgac atgatctgtt
    17461 tagcagcagc ttggatgttc cgggctcttg agtaataacc aaggccctcc catgctttta
    17521 aaagcttttc ttctggagca tttgctagct cttcaattgt aggaaaccat tccatgaaac
    17581 gataaaaata atcgatcact gtgtctactc tggtttgctg aagcatgatc tcagagatcc
    17641 agatacgata aggatcacga ttatagcgcc aaggcaggtt tcttttttct tgttcatacc
    17701 attgaatgaa ctgatcttga aattcttttg tttctttatc tgtccatttt tcccattctt
    17761 tattcacttt cctcttcctt ttccgtaatg aatcgctgga tcatatccaa atcaaatcct
    17821 ttttgatata agctttgctt tacttttaga tttctttttt taggttcaaa ccttaaattt
    17881 cttcgccata gtttatctcc ttgtttgact aaataatcat attctatttc ttgatctgct
    17941 tcttgcggaa gatcagctat cgcttgttgg atcacttctt gagaaaagcc tttcgtcatc
    18001 aatgtttgtt ggatttttct caataattct ttttggcttt ttccgtgatt ttttccaaac
    18061 gctttttcgg ctgtctgatg tgcattttga acttggtctt caaatgtata ttggctgagc
    18121 gcttgttcaa tgatttcagg tttcaaacct ttttgctgca tctgctgcgc taatttcttt
    18181 ggtcctttgt cacttaagcg catattcgtg cgcacataac tttccccata gaccaaatca
    18241 tcgacaaggt ccaattcttt taagcgggct acgattttat ttcgatcttc ttgaaggatt
    18301 tccttttctt ttaagtatgt tcggatttct ttttccgatc gcaactgata acttaagtaa
    18361 ttcattgcta ggtgaaaacc gaaatcagct ccgctactct tcttgatctc ttggatagtt
    18421 tcttcgtcta attcccgtcc ttttaataat cgatagcgta ctaatacatc ttcagatact
    18481 tgtaaagctt ctccttcaga taaatctacg cgatagaaag ggcctttgcc tttactgatt
    18541 ctcgtaacgg ttaacataaa catcctcctc gaacatatat tctctatcat accacagaaa
    18601 atccccgtaa tcaggtatta tatgttagaa taaaccacag aaaaaagaaa aagggagata
    18661 cttatggcag aaacaattgt caaaactgga caaacggttc ctttgaaaat caagcgttta
    18721 ggcataaatg gtgaaggaat cggttatttc aaacggttga tcatatttgt tccatatgca
    18781 ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa ctcctcgcta tgcagaagga
    18841 gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag tggcaccatg tcctgtttac
    18901 tatgaatgcg gtggctgtca attacaacac ttagcttatc aagctcaatt ggattttaaa
    18961 aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag caggctttcg atcttatcaa
    19021 cttttgccga cgatcgggat ggatgagcct tggcattata gaaacaaagc acaatttcag
    19081 ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt tatacgaagc aaactctcat
    19141 cagttagtcc ctataacaga ctgcttggtt caagaacctc gaacacaaaa agtaatgaat
    19201 actgttgttc aactattgaa taaatttgat gcacccatct atgatgaacg gacaaacagt
    19261 ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa ctggggagtt acaagttgtt
    19321 tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa tgatccgtga gatcaatgaa
    19381 cgactgcccg aagttgtttc aatcatgcaa aatgtccagg cacaaaaaac gtctctcgtg
    19441 atgggggatg aaacgatcca cttatgggga aaagaagcca ttgaagaaca aatcaatgaa
    19501 gtgactttcg atctttctcc tcgtgccttc tttcaactca atcctcagca gaccgaattg
    19561 ctttatggcg aaggaatcaa agcattagat gtccagccta atgaaacgat cgtggatgct
    19621 tattgcggag ttgggacgat cggcttaagt gtagcaaaac aagccaaaga agtacgggga
    19681 atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa atgcggaaag attaggatat
    19741 cacaacactc gatatgaagt cgggacagca gagcaagttc tgcctaaatg gttgaatgaa
    19801 ggttttcagc ctgatggtat catcgtagat ccaccacgta cgggtttgga taacgcattg
    19861 atccaatcat tattgaaata ccctccaaaa aaacttgttt atatctcctg taatgtttct
    19921 acacttgcca gagatctggt taatttaagt aaaaagttca agattgaata tttacagtcc
    19981 gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg tgaagatgac gagactcgct
    20041 gactaaatga aagaggttgt gaaaaagtct gccctgtatc aagtaataag aacagccaaa
    20101 caaaaatagc tcttcatgtt ttatgaatac tgtacttgtg accgaagagt taacaggagg
    20161 acactattta tttaaaaaaa gggttgtgac atagtttttg tcacagccct ttttgattat
    20221 tattggatat tcacgtctat cacttgataa aatgcatttg tcgtatcgct gatattccat
    20281 actgctaaaa tcacattata tccttttcga tcctgcggaa gtgtgatcgt ttggttgact
    20341 gtttttggtg gaacagttgc tttgtcgtct atttttgtga tcaattcaaa atcactaaat
    20401 ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt agtaatccca agtacttgtc
    20461 cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc ctggattaac catgttttta
    20521 tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc cgctgactcc tgcactagca
    20581 atttttccat caatgaatgt atttttggta gcttcgatac tttgcggttc ccattgtgct
    20641 cttccgacat tttgatttaa gtttcctgct gctgatgttc ctaggtatgc acggcttcct
    20701 ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa aaccgaacaa ccctaaacta
    20761 actaacccaa taaacaataa cgtactaatc tttttcatgt agaaacctcc ttcatttgat
    20821 aaccacattg taaacgcttt attgtattaa cacaaacatt atgcttttat aattttatgt
    20881 tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa cagaagtaga cgagaaaagt
    20941 acaaatagaa aacatgagaa tcaaatcata tgatcttttt caaaaacatc attcatataa
    21001 acaaatgttc aactttttaa taacaacaat tatcaatttt tttcttactt cacaaactaa
    21061 atacaggtct tgctgtgagc cttcatggta aactaatatt atagaaagga gttagtaaaa
    21121 tgaagatgat aaaagatatt cgtttttggg tttgtgttat aggaattgtc atattaggct
    21181 ttttatcagg gttgcttagc ggaaatcccg gaaaatatta ctattctttg caacttccac
    21241 catttgcacc acctagctgg atatttgggc cgatgtggac cttactgtat atattgatgg
    21301 gtatttctct ttatttgtta cttaatcaca acaacaaaaa acagcgaaac aatcttgtcg
    21361 gtctttttgt gattcagttc atttttaatt ttatatggtc tgctctattt tttaatttaa
    21421 gaaatatttt cattgcagca atcgatatta cacttttagt tattttcttg tcagtgctta
    21481 tgtatcagtt gtggctgcat catcgcctgg caatgtggct catgattcct tattatttat
    21541 gggtactatt cgcaactcta ttgaattatt caatttattt tttaaactaa gcgctaaaaa
    21601 gagacgtcct gtaatttaac acatagaaaa agtgccacct aagttagatt ttttgtctaa
    21661 tttaggtggc actctttatt aaccaaacta tttttttcta gtacccactt gttgttccat
    21721 actcattatt ctggtcataa ccataaccat tttgttcctg tccagcgtaa ggatcataac
    21781 tttgagtatc ttgacttggt gtatagtact gacttgtatc ttctgtttgc tgataatagg
    21841 tattcggatc ttggtactga tcatatgtgt tttctgaata ggaagaataa tcggtgtacc
    21901 cttcactatt cgatccatca ccgtttgtcg aatcgccatc ttccgaatca tcaaagaaca
    21961 tcattgaagc attctcattt ttcaaattag gtaatgtatc gcttgttttt atatttaatt
    22021 gtttcttcag ttcattttgg atcgaaagaa gatcattttt tcctaaaatt tgatagtaga
    22081 tatcattgat catttgtcct tcgccttgca actggtcttg tttgattgtt tcaaacgccg
    22141 gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc taagtccgtc gtcacattgt
    22201 tctcaactgc ttttaaaatt tttctataat tgcttacgcc atctaaactt aaaactttat
    22261 tgacgatttt cgtcactact tcccgctgac gacgttgtcg accgatatcg ccttctggat
    22321 cttcgtgtcg cattcgagaa taggctaaac ctttttctcc attcaatgtt tgttcaccta
    22381 ctggtacatg gacaccatcc aaagtaaagt caattttatt attcactttg atgccaccga
    22441 ccgcattgat caaatcttcc atgccatcca aattgatcgt tacataatga tcaataggaa
    22501 tatcgagcag ttgctcaatt gaatccatgg ccatttcgac accaccaaat gcgtaggcat
    22561 gatttaattt atctaccgtt ccataaccta cgatattcgt ataaatatct cggtccaaac
    22621 tgatgatcgt tgattttttt tgtttcggat taactgttac taccatcatc gtatcagaac
    22681 gtccttgttc cgttcttcct aatccaccgg tatctaaacc taatagcaaa accgaaaaag
    22741 gttctttgtc atcaatgctt actctctcag aacgtttgga ggatattcga ttagagtttt
    22801 tagaaatttt gtttactgtt tggctcgctt ctcccataaa tctgattccg tatgctgata
    22861 atccggcaat cagcaaaacg aataccccta acaaggttaa tagaactttt tgccatcgct
    22921 tcataataca cctctgtttc acattttcta aaatattgtc tcatataact gttcaatcag
    22981 caaattcact ttttaatcat aagaaaactt taaaaaacat ttttgtgtaa cgattattat
    23041 tttttaaaat ttctcaaaat tcttaactat cataccatac cgtgaaaaag atcgaaagac
    23101 cgaatacacg cagttcgcaa tcgttttatc tttttttatg tattttttcc agaaaaagcg
    23161 aacaacaagg tattttccaa ataatcgtgc agtaaaatga tcgcacatac ctttcccatc
    23221 tttttaacag tctttgccta ccgttcagat tatcattcga tttcacgaca atttcttcct
    23281 atgactggtc cttttgttaa acccatcttc tattcgtttt atttccagct cactgcatgg
    23341 ctcttaaaat gactcgaact agtgattgca ttaccatcat cttttaatgg atagatatgc
    23401 tcctcttcaa agtaaaacga ttttctttta aagtaataga attgacatgg taatgagtat
    23461 gtatactagt ggtttcacaa atggtctttt attcaacatc caaaaactga aatttcttta
    23521 aaatccatta ctaacgaaga aaatgttcct tgcttgataa ttagttagga catgtcaagt
    23581 gaacaaatgc atttacttag ccaaaaaatc taaaaacttc ataaatagac aaaaagacac
    23641 gactctctaa gaagtcgtgt ctttattcaa caaattatta taatttagtc acttcagcag
    23701 cttgaggtcc gcgtgcacct tcaacaatcg tgaattctac ttcttgacct tcttctaggc
    23761 tcttgaagcc gtctccttga attgctgaga aatgaacaaa tacgtcatct ccgccgtcta
    23821 ctgtaataaa cccaaatcct ttttcattgt tgaaccattt aaccgttccg tgttccataa
    23881 atgtatatcc tccaaaaaag ttcttgcaag taaccttgca agaacattat acccaattta
    23941 gaaaacgcta gcaagctttc gctcttttat tttccaaatt atttttaaga ctgatatgac
    24001 ggaggaaatg gttattttgc tgcttcgaaa cgtttgttta cttcatccca attaataaca
    24061 ttccagaatg ctgcaatata atctggtcgg acatttttat atttcaaata ataagcatgt
    24121 tcccaaacgt ccaaacctag aattggtgtc tttccttcca tcaatggaga atcttgattt
    24181 gcagtagagg taatagctaa ttttccattt tccattacaa gccaagccca tccagaacca
    24241 aatcgtccag cggctgcttt tttgaattct tctttaaaag aagaaaaatc accaaaagct
    24301 tcattaatcg cttcttttat ttctcctgta ggttcgccac cagcatttgg tgccatgatt
    24361 tcccagaaaa atgaatggtt agcatgtccg ccaccattgt tacgtacagc tgtcttgata
    24421 tctgttggaa tagcgtccat atcagataat aattcttcta ttgttttttc gcctaattct
    24481 gggtatttct caattgctga atttaaattc gtcacataag tattgtgatg cttatcatga
    24541 tgcagatgca tcgtttcttc gtcaatataa ggttccaatg cgtcatatgc ataaggtaga
    24601 tctggtaatg tgtaagtcat aaaaaaattc ctcctcaatg ttttcttaat aatgtctaca
    24661 ttacaaagag taacaaagag aaggaaattc ttcaattgtt atgcctagtc gtctttttca
    24721 ctttgaccta cttcttcttc aaaaacctca gtttctttaa aaataaacaa cttgctgaaa
    24781 atatagttcg caaggataac aataatttga gtgatgattt tcgccacata ctcattgctg
    24841 ttcataacat tgatcaacaa taccattgcc cccatgtcca atccgtaaga aaggatacgg
    24901 tagaagaaga acttaccgca ttcaataagt gcatgtgtaa aattcggtga ttttgaatgg
    24961 aatacccaaa gtttgttcgt tacaaaagca aacaatacag aacaaaacca cgaaatagaa
    25021 ttggataagg ccatacttaa atcaaaatat tggcgtgcga gagcaaaact aacaaaattt
    25081 acaatcgtag ccaaaccacc aaaaaagaga tagactagta tctcccaata tccttttgct
    25141 tctaaatatc cttttaactg tcgatactgc ttcatgagcc aacctacctt attaaaaatt
    25201 cttatttatc atactctata tttcaataac tttcatcttt atttaaaaaa attaattttt
    25261 tttgatcctt tgcctttgtc tctgcttgga tcgtcacatg tgtaatacca tattttaatt
    25321 ttaataaaga aattatacga ggatatatct tttcaatctc gcttagtagc tgatcttcaa
    25381 aatcaatatg tccgtcgaaa ataattatct tttcgctata ctgccaaata tggacatgat
    25441 gaacatcctt taccccatcg attgcttcaa tttcttgttc cattttttca tagtcgatag
    25501 caggtgcact ttgtatcaaa atagcggcag cttttttgat caccattatc gcttctatca
    25561 atatatatat cgaaatgatc agtgtaataa ctggatcgat ccagtagata tcgaaaaaac
    25621 ggatcaaaat tgctccgata atcacactta ccgaagacag tgtatcactt aacaaatgca
    25681 aataactaga ttttatattc aaactttcat ctgcttcttg tcgtaataat aatactgaga
    25741 acaaattcgc gagcaatcca ataactgcta ctgttagcat caaatgacta tttatctttt
    25801 ctggtgaagt gaaccgcttg aatgcttcaa cggttaaaaa caaggataat atgatcaata
    25861 tgaatgagtt gatgaaagca ctcaatattt ctaaacgctg gtagccatac gtacgttttt
    25921 tatttgcttc ttttcgggcc attttttgtg ccccgtaaga aaaaataatt gccaatgtat
    25981 cactaaggtt atggaaagca tctgaaacaa gagacaaact ccctgacata atccctccaa
    26041 taaattctga taaagtgatc accgcattca aaaccactac tccaaatagt ttttttgatg
    26101 atctctgtgg ctgtgtaggc actattcctt cctccatttt atttatattg gatagtcttt
    26161 agacaagaat aacatttttt tgatatttca ccaatcattc agctgtttcc ttaaagcact
    26221 tgtatttcac ttgatacttc gctagaattg caagtgatga cactactaga tgaggagtac
    26281 ttatgacaac aaaacaatta attatcagtt cctttatacg attcaaagac ctaaaggagg
    26341 cgagaaatgt cccattttgg aaagccattg tctatctttt agttttgagt ataattatgg
    26401 ctttgccaat ctcttttcaa gtttttcaag tcttaaacaa cattcaattg gatgggcaaa
    26461 agattgctac tcgcattcct gattttacaa tccaaaacgg gcagcttcaa accgaagaaa
    26521 aagaaggatt catttaccaa acaaactcca ttatcttcac atttgatcca gagggaaaac
    26581 gaacagaaca ggacatctct actgatttga tggggaattt tgttagtgtc gggatgttaa
    26641 aaaacaaact tatcatagct cttcctaata caggcacgac ttctgctttg ttgaacaata
    26701 accaattaga acttccctac accaatgaat ctttaaaaaa cttaacaggt aagcagctcc
    26761 gttctttttt aagtgaagca tctataccac tttggataaa agtcctgaca tttatatttt
    26821 ctatttatcc tagttttctg aatctgctga tcactttgct atttaccaac attgctgctt
    26881 ttctctatgc acgctttcga ctaacaaaag ctacttttct tgattgttta aaaacattga
    26941 tctattcaat ttcattgcct actataattg caacaatctt gatggtcttt cttccttctt
    27001 ttgacactag cgccttcatt gcttttgcag gaatctttat ttttgctcaa gctgtcaaag
    27061 gatgggcaaa aatatcgatc aattaagaaa taaagacaac tcgttatttc tacttcaata
    27121 gaccctttta gaggttgtga catttttttg tcacagcccc ttttttattt gaaaaatatt
    27181 tctttctcaa aaaacaagca aggcgcttca aagttcttct tgtatgcgct acacttagag
    27241 taaagaaagg cgggaaatta actatgtcag aaagaatcat ttatgcagat cgtccatttc
    27301 gtgatgaatt tatcgaagaa atccatacta tagcgcctgg ttatatcttt aaaacagaat
    27361 tacaaccaga ggatctacaa aatgtagaga tcagtttggg ctggaactca gactaccaag
    27421 aaaaactatt agcctcttct accttgaaat gggtccaatc tatctcagct ggtgttgatc
    27481 agctacccct tgagactttt tcaaagaaac atattttact ttcaaatggt agtggcatcc
    27541 acagtgaatc gattacggag catgtcttag gaatcatcct tgggtattct cgtggtcttt
    27601 ttcaagcaca aaaggcacag agtgacatga aatggttagg ttcatcggtc cattaccaat
    27661 ctatccaagg gaaaaaaata ctaatcattg gaactggcca tattggaaaa atgattgcaa
    27721 aaaaatgcaa agcattagac atgaactgct atggaatcaa tacatccggt catccagcag
    27781 aaggaatgga aaagactttt tctttaaatc aactaaagga agttcttcct gaagtagatt
    27841 tcgtagtcaa tatcttacct ctaacggagg aaacaactgg gctctttgat cagcatatgt
    27901 ttgaacactt tgatccaaaa tctgtattca tcaatgttgg ccgtggtgct tctgttaaga
    27961 cacaagatct ggtgcaggct ttaaataacc agcaactttc ctttgcagca ttagacgttt
    28021 ttgaggaaga gcctcttcca caagatcatc ctctttggaa aatggacaat gtactgatta
    28081 cttcccacat tgcaggtttg acacctgatt ttcaaaagaa attaatggct atttttttaa
    28141 ccaatctgaa aagctacttt gccacccatg agcttcaaac caatcaagta aaattgtctg
    28201 caggatatta atagctgaaa acaatatttt caaaagaaaa aagagaaggt gacaaaaatt
    28261 gtgtcaccct ctcttttttt attgtttttt aaagtgatag gttggctcct ccaggttggg
    28321 atcataatca acaagtaaat catatccttt aaagaaccat tcatctgctt cctcgataaa
    28381 aaaaagtgtc ccatcaattt ccactttctt tacaggatgt tctggctgtt caactgacat
    28441 tccaatcgaa aaaccatcat gaacctctgt tttgccgtag acttttccaa aaaaacgtat
    28501 tccgtaagtt tctggcagat caatttctga gtcaaaccac ttttgtgctt ttggcgttac
    28561 cgttaatttc atcccaacaa cctactttct cttttctttc tattgattca tcttatcgtc
    28621 tagaatatcc acatcgcctg tctctccaat aaccagtaga ttatcatttt cacgaacata
    28681 ttcatctgct gcaggagaag cgataaccgt ttgatctact cttcgtatgg cgactactgt
    28741 taagtcaaaa cgttgcctga aatttaattc agctaatgtt ttgttgaaaa atttaggatt
    28801 ggagactcgg atttctgcta aagaatattt atctgacaac tctaggtaat ctaaaatatt
    28861 ctttgacact aaattatgcg caatccttat tcccatgtct cgctcgggat ggacgacgcg
    28921 atcagctccg atcttttcaa gtactctggc atgatactca ttttgggctt ttgctaaaat
    28981 atttggtacg cccatttcct ttaccatcag agtaactaag atactggctt gaatatcttc
    29041 tccaatagcg acaattacat gatcgaaatt tcttatgcct aatgatcgaa gtgtcatttc
    29101 atcctgtgca tttgccacaa cagcatgagt ggctatattc atatattcat tgacccgatc
    29161 ttcattacta tcaatagcca gtacttcttg accagcttcg atcagtgttc ggcagatact
    29221 tccgccaaat cgtccaagac ctacaattgc aaaattttgt ttcaccgttt tcctccttta
    29281 gtatcttctt ttctcagtat aacaaagcct ttacaaaaac aaaagcaagc atcattattg
    29341 taaaaaagcc aagaaaaata atagttattt tctcagcttt tgattatcct ctttatcatc
    29401 gtttttattt caccaaacca tgtttgaatg catagatagc ggcttgtgta cggtcttcca
    29461 cttctagctt agccaatata ttggaaacat gtgtttttac tgttttcagt gtaataaaca
    29521 attcgtccgc tatttcctga ttactttttc cttcagaaat cagcattaaa atttcatttt
    29581 cccgattcgt tagttcttca tgtaatacag gctcatttcg ccgactcatt ttttccatca
    29641 tttttgttgt cacttctggt tctaatactc gctcgccacg ttgcgttgcc cttatagcat
    29701 cagcaatttc atgtgcggta gaggtcttca acaaataccc tgctgcacct gcttcaatgg
    29761 caggataaac tttttcgtca tcaataaaac tggttacgat aatgatttta gcttctggcc
    29821 aatcttttaa gattgctttt gttgattcga taccatccat ttcttccatg actaagtcca
    29881 ttaaaatgac gtccggtcga agtgtcatcg ccttctcata gccttgtcgt ccattttcag
    29941 cttctccaat tacttcaatg tcttcttgaa tagataaata aaaagagacg cctaagcgca
    30001 ccatttcatg gtcatctact aataaaactt ttatcatcct tatctatgcc tccttcatca
    30061 aagggacctt gatttctaca cttgtacctt gacctttaaa actaatgatt tttactgttc
    30121 caccaatacc agctactcgt tctttgatat tgttcaatcc ataacttccc gttttcgttt
    30181 cattcatatt aaaccctgtt ccgtcgtcaa taatacgtaa caaaagatta ttgtctattt
    30241 tgtgcaaata tacctctaat tcattggctt tagcatgtct taatgtattt gataacaatt
    30301 cttgaacgat tcggaacaag tgatcctcaa tggaactaga tagttttaca tcttctacat
    30361 cccatttcag cgaaatctgg attttatttt gcaattcctt caataactgt tcgatacctt
    30421 gctttaaact tttctcttct aaattgactg gacgtagatg aagaagcagc gcacgcatct
    30481 cagactggga agcgttgatg atctctgcta ccattttcaa ctgcttgcga aacatttctg
    30541 gtgtctcgct tttttctgct tgctccgtca atgccgacat catcatcatt gctgcaaaca
    30601 attgttgcga aactgaatca tgcagctcac gggctaatcg atgtctttct aattctaaaa
    30661 tttcttcttt ggtttgtcca tctacatatt ggggacggct cgttacaatt tgtaattcac
    30721 tagatacttc aatcattttt tcttttatct tagtgatttc cttatcaata tctttgatgt
    30781 ataaatcgtc cgatgcactt gggatcggaa gaagcaacaa tttggattca taattgccag
    30841 aagaaagtgc acgcagcttt tcttcgattt tcccgtattt tgtcttttga ataagtgata
    30901 ataatagaaa gacaattaat cctacaccta aggaaatggc taacaaatgg aagataagcg
    30961 gaacatataa taaacgtgca cgcagtaatt ctccccacca atgactttga ttgctggcat
    31021 aaaaataagt gaaaagtgaa aatagaataa taaggaaagc cgcaatcccc gaataaacag
    31081 ctagcatcgc tctggatatt tttcccatca tattcgtatc acctcgacat ctccaatcaa
    31141 ggtattagta ataatcttca atctacgagg gttttcatca taatcactgc tgtagatttt
    31201 gatttgttca tttttcaaag aaaattgctc ttcttcaaac aagacatttc ctactaatgt
    31261 cgcatgctct aatcggatgg ctacgcccaa aggtactaag atccgtgttc tgcctatccc
    31321 ttttcttaca atcacaatat tatcatcttt tggcaaaagc gtattgccca aatctatgat
    31381 agtatcacca gaaatgacgg cgatgttgat atcgtcccat tcatagacgt cgtttccaat
    31441 acgttggtta ccgaataatt gctgtctttt ccgctcatta ttatgtgttt ttaattgctc
    31501 tgtttggatc atcatgatct gtttctttcg ccaaaaagca tttttggtta aatctatccc
    31561 tgaaatctct acacctttca gaccaataaa taaaacggca aatacagcca tcatccataa
    31621 agcaggactg ttgactaagc taaaaaaaat gaccaaactt cctaatatca attgaaaatt
    31681 ttgaaattta gtacgaggat gtttgcgcat agctagatat atattgaaaa taccgaatat
    31741 cactaaaagc aaaagtccag tattgtttac gatttgccaa acagcaaata acagcagtag
    31801 tgcttcaact accacaaaaa aacgccaaga actattcatc gctctttctc cctcctgaca
    31861 cattctcatt tcatcatacc tattttttta actgttggct ataggacttt agatgtaaga
    31921 tcgtttgttg aaatctaaaa tacacaaaga gaaaaagatc tagagtgtat catcagtaag
    31981 atgaactctc tagatctttc agtcagacta cgcttgttct actttgacga ttttaactaa
    32041 catatcgcca cctggagtag caatagttac ttcatcattt ttacgtttgc caatcaatgc
    32101 ttgcgcaata ggagaatcat tggaaatttt gccagaaaac ggatctgctt cagcgcttcc
    32161 gacaatcgtg tactcttctt cttctccatc tggtagttcg atgaatgtaa ctgttttacc
    32221 aattgatacc tcatctgcat ctacaccgtc gttatcgatg atttgagcaa aacgaatcat
    32281 attttcaatt gttgtgattc ttccttcaac aaatgcttgt tcatctttcg ctgattcata
    32341 ttcagagttt tcggaaagat ctccgaaacc gcgggcaatt ttgattcgtt caatgatttc
    32401 tccgcgtttg actgttttta gttcttctaa ttctttttct aatttttctt taccttctag
    32461 agtcattgga taaactttct ctaccatgtg taacgtctcc ttttttcgat aaaataccgc
    32521 aaaaggtaat cttttgagga atatagtcca aaaaagatta ccacgctttt cttcaaatgt
    32581 aaatgttttt ctaaaaatga tcaatgtcta gagcctgttt ttctaagaat tatttacata
    32641 tttttccact aattccatat gttcttcata agttttagcg aaataaacat ttccagtgct
    32701 cgtatctgct acgaaataat aatagtcatt ttctgctggt tctaatactg ccttgattgc
    32761 ctcttcgctt ggattgtcaa aaggtccagg tccatagccg gtattggtat acaaattata
    32821 tggtgaatct acagctgtat cttcatacgt cactaattct ttgtgttcac ctaatgcgta
    32881 taaaacagag atatctgact gaagtggcat atttgctttg attcggttaa agaagacttg
    32941 tgcaatgttt ttacgatcat tttcttttac gccttctttt tcaactagag aagctagagt
    33001 taaaacttct tgtacagtca aatttttctg cttgatagct gaataatagt tttgcatgac
    33061 cgtattcgtc ttgttcacca tttcaatcac tagatcctta agcgaagtac tcgtgtaata
    33121 atcatatgtg gctgggaata agtaaccttc caaacggtat tttgtgttct cagctttaga
    33181 tgcactttcc aacagttctg ggaatttttg gtgcaactcg ttaaagaaag tctcatcatt
    33241 cactaaatct aaaaattctt tcttgtcttt accagttacc ttcgctaccc gttcagcaat
    33301 ctgatcgata tcgtaccctt ctggaatggc aattttccca tctgcaactt ttgttggctc
    33361 agaagtaccg ccttcttgca actgtttgcc aatctcgtca agagtcatat taggcgctaa
    33421 ctgataatag ccagcttgaa agccagttaa atttttaaat tttgtataat aattgaaaac
    33481 aatcccgctt ttaataatat tgtccttctc caaaatctca ccaatctgct tgttagaaga
    33541 gccactcggg atctcgactt ggactaattg gtcatctgat ttatctaaag gcttcaaccc
    33601 gctatctaca taacgataca cggtaaatcc aagaaatccg ccaataatca ataatgtaag
    33661 agcaatcact aaaatgatct tcctcacaat cttatcttct tttttacgca gatttctccg
    33721 cttgtcgatt atatctgctg tagctgcctt actggcttct tttttatctt tacttgggtg
    33781 ttgtttgttt cctgacaaaa caactcctcc ttactatcaa gttcgtaact ttattcatta
    33841 tacatgatat ttgaattggc gtgaatgatt ttatttaaat attctatcaa aaatgtaagt
    33901 agtaaatcca ttactataag attaaaataa atgttatcaa gatacaagct attttagctt
    33961 tattctttct gtaaaaaaga aaggagacta aataaactat tatgaaaaaa ggaaaacatg
    34021 ctaaaaaaag aaaatattca tttttaatga tcgttgtcgt gttgtttagc cttagtgcgt
    34081 tctctacagg aactattctt ggatggcaat tttacaataa tgaacactct cagatagttg
    34141 cccatgctca atcagctcaa aaaaagttga tactgactac caatgaacaa cagctcgttg
    34201 aaaaaatcga tcaactatta agaaaaaatg attatattgg ctcaatttac gtaaagaaaa
    34261 ataatcgcat catcatggaa aaaggctatg gctatgcgaa taaactcacg caacaaccaa
    34321 atgatccttc tctttattat cagattggtt cggttcaaaa agcattgact actcttttga
    34381 ttttaaaaca agtcgagcgt gggcaccttt ctttagatac taagctggct caattctatc
    34441 cacaaattgc tggcagccag caaattacta tccgtgactt gctgtatatg cgctcaggct
    34501 tgcaccgaac tgcttctcca accataccga tgactgatga agaagtcgtt caatttgcga
    34561 ttcagcactt aaaatttaca gattatcaga cctctcatta tgattcattg aactttacca
    34621 tattgactgg aattttgatc aaattaacaa atcaaccgta tgaaaaatta ttgaccgatg
    34681 atatcatcga acctttagga ttacagcata caaaatttta tgaccaagtc aaaaattcat
    34741 ccaatcatgc actatcctat caaatgtcta aagaggatga ttactttaaa ccactgacag
    34801 aatctgaaac agagatccat aacgaattag ggactggtaa tatcagtatg tccgtctatg
    34861 atttaaatac gctgtttaca caagttttat ctggaaaact agtttccaaa gacttactct
    34921 tctcattatg gcgtgaaaat gcgaatggac atccatatag tggtggggta tatagcggag
    34981 ataattatat cctgtcgcaa ggaaatatcg actcttttga tacatctgct atatttagca
    35041 aagattcaca ggatgctatc atcatggaaa gcaatataaa agctgacaaa actatcaaac
    35101 tgcctactgc tgatctaaga aaccaaatct atgacctgat ggaaggaact actaagttgg
    35161 caaatagctg atatgatgaa aagatagcaa atactacttc tttgaatagc atttgcttaa
    35221 aaaaagtact cgactaaacg taggaaagag tgtgggacaa atctaaaaat caatttttgc
    35281 tacagacttt aattccaaat aaacagaaca gaaagaggtg ttcttctttc tgttctgtcc
    35341 aaattattct aagatttctc tttaccaaca ttatttgaca aagggcctat taaatctaaa
    35401 atagtatctt tagattgcta aaatatgtgc tagcaaatca ttcatggctt gttcttattt
    35461 ttaattttat gcaataaaaa agaagaacaa ttagcgtgat tatagaaata aataatgaga
    35521 ttttagaaaa aaatgtgtat gaatacctga ttgttacttt atttattcca ctttcacttt
    35581 ttactgtaac aaaaggacct ccttgtttta gtactgtagc attttttgaa ttaacagtaa
    35641 ccgttaatcc tttataccgg aatacaggta aaactattgt tttgcctttt gaagatttaa
    35701 acgtaaatgt aatttcatct ttatttggag aaaagctact ataaataact tgaccatctt
    35761 ctgtaacttg atggtttttc aaaagcaatt gatctttttt atagcctata ggtacataat
    35821 caaaattctc aaattcttca agtttatttt ccatttgttc attagaataa gttccttgaa
    35881 aattcatttc tttcaccatg ttaactactg atgaataatt taaaattgaa atagctaata
    35941 ttaataatag tataaaattt cttctccttg tataagaaaa tttttccaac tgtggagaaa
    36001 taaatagaga tactgaaaaa gatatgaaca atgttgaaaa tatcataaaa cgccaaggag
    36061 cttgaataat atttaaagga gtattttgta aggcaaacca tggaaatatt tttgtacaaa
    36121 gtagaataga tattataccc attatataag tgattttaga taagattcct aattttttaa
    36181 agttaaatgt tagtataatt aaacaaataa gcaaagttaa gccaatacca aatgatagta
    36241 attctgaatc tatacttcca atcaaaaact ctttacccag ataaattgct cttttttgta
    36301 atatatccac ataaggtcta ttcagatctt gatacaatgt ctgctcaatc ataggaccta
    36361 taaacgctag tgaaagaagt aaagtaacta tagcagcttt tattaattta ataattaaga
    36421 agatttctat tttctttttc cgataaaatt gaataattaa agcaattata atcattacca
    36481 tagtcatagc tacagaaaga acatgagtgt aaattaataa agtcatgcta atcgccaaca
    36541 taacccaaga ttcttttttt gagtcgtaaa gctttataaa cccgagtaaa ataagaggaa
    36601 gtattgacat tgcgagtaat tcccccattg cccctcgaag aaatatttct acactcctaa
    36661 aagaagatgt tgtatatata atagcgaaaa aggagctagt aacatgtttt ttggtaattt
    36721 catacattac ataatgacat ataaatagag taatgagcgt aacaatataa agaaaaaggt
    36781 tatagcctat tgctaaatta gtaaacttga tcaataaaaa taaaggatat agagttaacc
    36841 atgggtaaaa attatttact tgcatgccat atgatctaaa cgtttcaaaa ttaatagggg
    36901 agctaaaaat agattctaaa cttaacgcac gattaatgtg aaatgccgta tcataaaagc
    36961 caaatatttc catgttttta aatgctggaa ataaatataa taaactaaca aatgcataaa
    37021 ctataaatat ttttacagaa aaaatttttt tcaaattcat ataccacctc atgttttttt
    37081 atattacata tagtatcatt tatttttttt aaacactaga ttagtcacta tataaaatgt
    37141 gattaatttt attattaata aaatattttt tatattacaa aagtagtgtt t
//
LOCUS       DAZKSR010000009        36568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000009 DAZKSR010000000
VERSION     DAZKSR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.009"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8XG_000473"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8XG_000473"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0772416.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8XG_000474"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8XG_000474"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0772417.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8XG_000475"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8XG_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0772418.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8XG_000476"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8XG_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0772419.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8XG_000477"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8XG_000477"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0772420.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8XG_000478"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8XG_000478"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0772421.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8XG_000479"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8XG_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0772422.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8XG_000480"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8XG_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0772423.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8XG_000481"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8XG_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0772424.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8XG_000482"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8XG_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0772425.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8XG_000483"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8XG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0772426.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8XG_000484"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8XG_000484"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0772427.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8XG_000485"
     CDS             15402..16376
                     /locus_tag="ACG8XG_000485"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0772428.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8XG_000486"
     CDS             16546..17946
                     /locus_tag="ACG8XG_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0772429.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8XG_000487"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8XG_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772430.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8XG_000488"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8XG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0772431.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8XG_000489"
     CDS             19454..21253
                     /locus_tag="ACG8XG_000489"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0772432.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8XG_000490"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8XG_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFQ0772433.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8XG_000491"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8XG_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0772434.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8XG_000492"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8XG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0772435.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8XG_000493"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8XG_000493"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0772436.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8XG_000494"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8XG_000494"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0772437.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8XG_000495"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8XG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0772438.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8XG_000496"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8XG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0772439.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8XG_000497"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8XG_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0772440.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8XG_000498"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8XG_000498"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0772441.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8XG_000499"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8XG_000499"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0772442.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8XG_000500"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8XG_000500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0772443.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8XG_000501"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8XG_000501"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0772444.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8XG_000502"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8XG_000502"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0772445.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8XG_000503"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8XG_000503"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0772446.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6705 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccctttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAZKSR010000010        36541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000010 DAZKSR010000000
VERSION     DAZKSR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.010"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACG8XG_000504"
     CDS             complement(29..1261)
                     /locus_tag="ACG8XG_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0772447.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACG8XG_000505"
     CDS             1516..2085
                     /locus_tag="ACG8XG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0772448.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACG8XG_000506"
     CDS             complement(2263..2703)
                     /locus_tag="ACG8XG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0772449.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACG8XG_000507"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACG8XG_000507"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0772450.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACG8XG_000508"
     CDS             3657..4580
                     /locus_tag="ACG8XG_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0772451.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACG8XG_000509"
     CDS             complement(4805..5044)
                     /locus_tag="ACG8XG_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772452.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACG8XG_000510"
     CDS             complement(5049..5468)
                     /locus_tag="ACG8XG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0772453.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG8XG_000511"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG8XG_000511"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFQ0772454.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="ACG8XG_000512"
     CDS             complement(7040..7675)
                     /locus_tag="ACG8XG_000512"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0772455.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="ACG8XG_000513"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="ACG8XG_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="ACG8XG_000514"
     CDS             complement(8017..8652)
                     /locus_tag="ACG8XG_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0772456.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="ACG8XG_000515"
     CDS             9164..10306
                     /locus_tag="ACG8XG_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0772457.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="ACG8XG_000516"
     CDS             10299..10505
                     /locus_tag="ACG8XG_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0772458.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="ACG8XG_000517"
     CDS             10584..11777
                     /locus_tag="ACG8XG_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0772459.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="ACG8XG_000518"
     CDS             11982..13121
                     /locus_tag="ACG8XG_000518"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0772460.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="ACG8XG_000519"
     CDS             13114..13914
                     /locus_tag="ACG8XG_000519"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0772461.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="ACG8XG_000520"
     CDS             13914..14741
                     /locus_tag="ACG8XG_000520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0772462.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14746..15453
                     /locus_tag="ACG8XG_000521"
     CDS             14746..15453
                     /locus_tag="ACG8XG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0772463.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="ACG8XG_000522"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="ACG8XG_000522"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0772464.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="ACG8XG_000523"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="ACG8XG_000523"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0772465.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="ACG8XG_000524"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="ACG8XG_000524"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0772466.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="ACG8XG_000525"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="ACG8XG_000525"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0772467.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="ACG8XG_000526"
     CDS             19038..21071
                     /locus_tag="ACG8XG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772468.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="ACG8XG_000527"
     CDS             21115..22395
                     /locus_tag="ACG8XG_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0772469.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="ACG8XG_000528"
     CDS             22605..23411
                     /locus_tag="ACG8XG_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772470.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="ACG8XG_000529"
     CDS             23423..24643
                     /locus_tag="ACG8XG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772471.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="ACG8XG_000530"
     CDS             24633..26219
                     /locus_tag="ACG8XG_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0772472.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="ACG8XG_000531"
     CDS             26258..28396
                     /locus_tag="ACG8XG_000531"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0772473.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="ACG8XG_000532"
     CDS             complement(28764..29747)
                     /locus_tag="ACG8XG_000532"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0772474.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="ACG8XG_000533"
     CDS             30125..31516
                     /locus_tag="ACG8XG_000533"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0772475.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="ACG8XG_000534"
     CDS             31532..32572
                     /locus_tag="ACG8XG_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0772476.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="ACG8XG_000535"
     CDS             32591..33676
                     /locus_tag="ACG8XG_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0772477.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..34671
                     /locus_tag="ACG8XG_000536"
     CDS             33709..34671
                     /locus_tag="ACG8XG_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0772478.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            34664..36091
                     /locus_tag="ACG8XG_000537"
     CDS             34664..36091
                     /locus_tag="ACG8XG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0772479.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            36093..>36541
                     /locus_tag="ACG8XG_000538"
     CDS             36093..>36541
                     /locus_tag="ACG8XG_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772480.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKN"
BASE COUNT        11919 a         6117 c         7104 g        11401 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa agataatgag
    34021 tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg aatagatcta
    34081 gatcaaatta aacataaaga attttattat aaacatatag atgtatatca aaaacatgat
    34141 tatcctagaa ttaataggaa taacacaact gcatttaaaa tttttagagc tataattaat
    34201 cgttgtctga ggatgattaa acttccccca aaaattagac accataaatt tgatttgtat
    34261 catggttctc aatggtgggc tttgtcgaaa gaatgcctaa cagaattaat acaaatgtat
    34321 gaacaaaatc aacaagacta cctgaatttt aaaataggta tgtttgcccc agatgaaaaa
    34381 ttttttcata cacttttttt taattcctcg ttcaaaaata aaaatgttat tggaggacca
    34441 gatttaccac ttgaattgaa aaatattgaa gaaactactt tacaaacttc gaaattagca
    34501 aatattcata ttattgatcc ttccatgaac aaatggtttg atgaaaggga tatagatcaa
    34561 ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat cggattactc tgataaacta
    34621 ttaaatagaa ttgataaaga aattttgaaa gttggaaata tagatgaata gaatagtacc
    34681 tgttttagag aagagtttat taagtcgaga tgcgtttcga gtaataaatt taatcatttg
    34741 tctgctttat tttatgccta gtacttttaa tgtaactaat atcccgatga aaatagcttt
    34801 tgcttggggt ggaattatag ttttgtatga ggtttttata aagaaaaatt tttttaaaat
    34861 gcgtttttct tggctactga ttggagcaat gattagtttt atcttaacta taatcattaa
    34921 cagagaaaat tttttaattc catctattta taattttgga tatttgctga ttactttgat
    34981 agtaattttt cctgcagact tttcacaaag tgaagaggaa aaaaagaaaa aaatggtgta
    35041 ttttaataat gttttcatta tcataatttt tttagctgcg ctaatatcta tctatcaatt
    35101 tatttttttg atttcttatc atgtcccaac aggaacgcca ggattacttg caagacaagg
    35161 ttttattgaa catcggttat ttggtgtcta tacaagtcca aatgttggag ctatgtttgg
    35221 atatacttct attattttaa gtttaattag tgtattaatt tcaaaaataa caaaaacgat
    35281 tcgtgttttt tatatgatga attttatcgt acaaatattt tatattacac tttcttcgtc
    35341 tagaggtgcc caggtagtaa tttgttcttt tttagtatta tttcttttgt tgtttgccaa
    35401 ttcaaaattt agaaagagca ttagagaagt actacctgta aaaatcaaat ggatggtaag
    35461 catattatta gttctattgg tatatttttc ttttggtact aattatgcta aggatttttt
    35521 agcaacaatt cctgtatcga ttgatagaaa agtagaagtt atagagtcaa atgaatcaga
    35581 taaaactcca cttccagaaa aagaagtaat attacagcat tctagtgata actccgaaat
    35641 ttcagctggt cgttttacga tttggaaagc tgcgcttaaa gttataagac caactttgtt
    35701 atttggttat ggagagaccg atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt
    35761 agatcttgat actacagatg tgaatgaact aaaaagagca catggaaaca tgcataatgg
    35821 atttcttcaa gtacttgtat caagtggtat aattgctttt atatgtatct atgctttcta
    35881 tgtgttaaat atctttagtt tactcaaaac attttttaga agtaaagtga attatgcgtt
    35941 tcttggtatt gttttaattt ttatactatc tatttttata gatgaaatgg tggaggctca
    36001 tgtacttttc aataagcgcg atgtcatttc aattgttttc tggtattatt tgggaagcat
    36061 ttcttacttg tatttaaaaa aggagaatta atatgaagac agctttcatt gcttggactc
    36121 ctttacattt gattaatata ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag
    36181 atttatttat ttattctgaa tttgaaaacg ctagaagata tagtgagcgt ataaaagaat
    36241 tagggctatt taatgatgtt gtttttgtga atcaccggaa aatcggttct tcctttaaac
    36301 gtaaaattaa cttgttaact aaccataatc aatttttctc ggatgattta tccgtttatg
    36361 atgaaatttt tatacagggg gaaaattatt tcacaaaaat acttttctcc aatgtttaca
    36421 aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg tttgggtact tatttgaaga
    36481 agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta aataaaaata
    36541 g
//
LOCUS       DAZKSR010000011        36520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000011 DAZKSR010000000
VERSION     DAZKSR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.011"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACG8XG_000539"
     CDS             complement(383..1849)
                     /locus_tag="ACG8XG_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0772481.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACG8XG_000540"
     CDS             complement(2140..4224)
                     /locus_tag="ACG8XG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0772482.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4366..4488)
                     /locus_tag="ACG8XG_000541"
     CDS             complement(4366..4488)
                     /locus_tag="ACG8XG_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772483.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAGAMIEEKKKE"
     gene            complement(4749..5108)
                     /locus_tag="ACG8XG_000542"
     CDS             complement(4749..5108)
                     /locus_tag="ACG8XG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0772484.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="ACG8XG_000543"
     CDS             complement(5138..5479)
                     /locus_tag="ACG8XG_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772485.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="ACG8XG_000544"
     CDS             complement(5476..6150)
                     /locus_tag="ACG8XG_000544"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0772486.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG8XG_000545"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG8XG_000545"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0772487.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="ACG8XG_000546"
     CDS             complement(8664..9854)
                     /locus_tag="ACG8XG_000546"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0772488.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="ACG8XG_000547"
     CDS             complement(9875..10402)
                     /locus_tag="ACG8XG_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0772489.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="ACG8XG_000548"
     CDS             complement(10449..13238)
                     /locus_tag="ACG8XG_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0772490.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="ACG8XG_000549"
     CDS             complement(13385..13585)
                     /locus_tag="ACG8XG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0772491.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="ACG8XG_000550"
     CDS             complement(14038..14358)
                     /locus_tag="ACG8XG_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772492.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="ACG8XG_000551"
     CDS             complement(14861..15979)
                     /locus_tag="ACG8XG_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772493.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16212..16334
                     /locus_tag="ACG8XG_000552"
     CDS             16212..16334
                     /locus_tag="ACG8XG_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772494.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16450..17757
                     /locus_tag="ACG8XG_000553"
     CDS             16450..17757
                     /locus_tag="ACG8XG_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0772495.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17878..19077
                     /locus_tag="ACG8XG_000554"
     CDS             17878..19077
                     /locus_tag="ACG8XG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0772496.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19104..20372)
                     /locus_tag="ACG8XG_000555"
     CDS             complement(19104..20372)
                     /locus_tag="ACG8XG_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0772497.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(20428..20494)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(20542..21183)
                     /locus_tag="ACG8XG_000556"
     CDS             complement(20542..21183)
                     /locus_tag="ACG8XG_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFQ0772498.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(21592..22095)
                     /locus_tag="ACG8XG_000557"
     CDS             complement(21592..22095)
                     /locus_tag="ACG8XG_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0772499.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(22218..22982)
                     /locus_tag="ACG8XG_000558"
     CDS             complement(22218..22982)
                     /locus_tag="ACG8XG_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0772500.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            23090..23365
                     /locus_tag="ACG8XG_000559"
     CDS             23090..23365
                     /locus_tag="ACG8XG_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0772501.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            23925..24875
                     /gene="yidC"
                     /locus_tag="ACG8XG_000560"
     CDS             23925..24875
                     /gene="yidC"
                     /locus_tag="ACG8XG_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0772502.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(24921..25322)
                     /locus_tag="ACG8XG_000561"
     CDS             complement(24921..25322)
                     /locus_tag="ACG8XG_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0772503.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(25336..26145)
                     /locus_tag="ACG8XG_000562"
     CDS             complement(25336..26145)
                     /locus_tag="ACG8XG_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0772504.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(26132..27022)
                     /locus_tag="ACG8XG_000563"
     CDS             complement(26132..27022)
                     /locus_tag="ACG8XG_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0772505.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(27035..27514)
                     /locus_tag="ACG8XG_000564"
                     /pseudo
     CDS             complement(27035..27514)
                     /locus_tag="ACG8XG_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(27690..28631)
                     /locus_tag="ACG8XG_000565"
     CDS             complement(27690..28631)
                     /locus_tag="ACG8XG_000565"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFQ0772506.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(28628..29623)
                     /gene="lacD"
                     /locus_tag="ACG8XG_000566"
     CDS             complement(28628..29623)
                     /gene="lacD"
                     /locus_tag="ACG8XG_000566"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0772507.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(29616..30812)
                     /locus_tag="ACG8XG_000567"
     CDS             complement(29616..30812)
                     /locus_tag="ACG8XG_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0772508.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(30830..31558)
                     /locus_tag="ACG8XG_000568"
     CDS             complement(30830..31558)
                     /locus_tag="ACG8XG_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0772509.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(31999..32973)
                     /gene="lacD"
                     /locus_tag="ACG8XG_000569"
     CDS             complement(31999..32973)
                     /gene="lacD"
                     /locus_tag="ACG8XG_000569"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0772510.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(32970..33428)
                     /locus_tag="ACG8XG_000570"
     CDS             complement(32970..33428)
                     /locus_tag="ACG8XG_000570"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0772511.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(33425..34888)
                     /locus_tag="ACG8XG_000571"
     CDS             complement(33425..34888)
                     /locus_tag="ACG8XG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0772512.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(34870..35802)
                     /gene="pfkB"
                     /locus_tag="ACG8XG_000572"
     CDS             complement(34870..35802)
                     /gene="pfkB"
                     /locus_tag="ACG8XG_000572"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0772513.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35944..>36520
                     /locus_tag="ACG8XG_000573"
     CDS             35944..>36520
                     /locus_tag="ACG8XG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0772514.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT        10689 a         7848 c         6210 g        11773 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt
    18001 gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg
    18061 attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg
    18121 ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg
    18181 ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta
    18241 ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc
    18301 acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt
    18361 tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata
    18421 aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac
    18481 tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc
    18541 gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga
    18601 atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc
    18661 ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa
    18721 caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca
    18781 accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc
    18841 tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga
    18901 caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac
    18961 gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt
    19021 tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa
    19081 aacaaggtgt gacaaaacat atgtcacacc ttgttttttt cttttattct cttgacgatt
    19141 ggcaagaata gtattccttt tttgacggct acttgctgtg cagaataaat accgctgttt
    19201 cctgaacaca taaagctgat caagcagatc ataaagaagt aagcagcagc ttgtgaacca
    19261 aataattcga ttcccatcac aaaacaggca atcggtgtat ttgtcgcacc agaaaatacc
    19321 ccgataaaac ctaatccagc aagaaaagga acagaaacat ggagcaaagg cgctagtact
    19381 gcgccaagcg ttgcaccaat ctcaaatagt ggtgtcactt ctcccccttg gtaccccgca
    19441 cctaaagaca agaccgtgaa gaataatttc ccaataaaat cataagcatg tgcctgtcca
    19501 ttaaatgatt cctgcagtaa cggaagactc aacccaaggt aacgttgtgt ttgcaaaatc
    19561 aacgctgcgg ctacaactac tgctccacct atcgtatttc gccaaacagg atgaggaatc
    19621 cattgcgcat accgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa
    19681 ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc
    19741 acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcatgaa atttgcaaaa
    19801 agcgcagcaa agaagctagg aaatattgct tctgttcgga ctttaccgat tgccaacact
    19861 tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg
    19921 attccactga taaccaaaat ctcccgttct gctttatcca atcggaataa atgcgctata
    19981 ttatcagcta atgctccccc catttgaact gctgttcctt cacgaccaac agatccccca
    20041 aataaatggg tagtgatggt cccaaacaag gtcaatggaa tgagccgcaa agggatcttc
    20101 tcttctcctc cattcccttg atcgatcact aagttattcc cacgactaga aagaccgcca
    20161 tgatacgcat aaagataagc aaaaagcgcg ccgctgactg gcaatagata aagtagccaa
    20221 ggatgtgcta gtcggatatc tgtcacgaac gtcaggctat ttaagaaaaa agcagacaag
    20281 ctccccatga atactccgag aatcgctgtg accaccagcc acttcaacat gtacatcaaa
    20341 atacgcactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaaa taaaaaaacg
    20401 cctactaaag aaaggggctg gccccagact ttagtaggcg tcattagcta tcttacgtta
    20461 gcatttaaag gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg
    20521 taaaaaacaa taaaattatc gttatttctt tacacgttca gcaaattcat agaaaggagc
    20581 caatttcgtt aactgttgat tacattgatc actatcatga caaatataat tccccttttt
    20641 cgtatacgtg ccatcagaac cggctttcgt tgtagaaaga aagagcgaga cattcgatgt
    20701 tttctgacag atcgcacaga ttcccttatt aatcgttggt gataaagtac cgctgatccc
    20761 ttttaatttt ccatcttcgt aataaaccat gaattttttt tgcgtccctg gatcattcca
    20821 accgatgtac gtatgtgctt gcaagtcgat ttcattccaa gcgggttgtt tgagtttttt
    20881 cacttttcgg aataatttct cgatttgtgg tttgctcggc tgcataaacg gctggacgat
    20941 ttgcttcagc tcctccaatg ctcgttctgc ttgcgttttt gtcagtttta catctaaaac
    21001 tgtcgttaaa aacgtatcga tttctgatgg ttcgtaacct aatacatcgc gaatcttttc
    21061 agctgctaga gcctgaaccg tttgtaccgt ttttgagtca ttgacagatg agtacgcatt
    21121 taataactgc tctacttctc tttttataaa aaaatattga tatggtttca ttgtttgttc
    21181 catgatttta aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt
    21241 ctgcaaagta tgtgttacct gaagatcccg gactctttta aataagatac gttttgcatc
    21301 ttatcatcac cccctttaat gttcattctt ctatgtacat gattcattcg ataaataaaa
    21361 gtaaaccgta tcttgagcag atagtcaact gcaaacagaa aaaggactgt gacaaaagtc
    21421 gtgtcaaagt ccctttctac aaacaaacgg tgttccagaa gtagcttctt caaaaaataa
    21481 gccggaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    21541 ctgacccact tctgtcacaa cctcaatatg gtctattatt ggttttacct gttaaatcga
    21601 ggattctttt ccgaagtaac gttgccatct cttacgcata cttgctgaca taggctgagc
    21661 ggcttctttg actgcgcagt atttgtctac gaatgttacg atatatcctt ctttgtactt
    21721 aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg atatctcgct caagatccga
    21781 caaatcggtg atcttttctg cattctttac agcgattctt ggatggatat acgcatgtgt
    21841 gccttcatca aatttcgtcg tacgccaatc atagtagaac agatcatgca aaagtccagc
    21901 acgcgctgtt gctttagcat tgccacccca tttttttgcg agcagataac tatagtaaga
    21961 aacactgatg gaatgctcca aacgagtgga atgaacatgc tgtgtgtaat tcgctaattt
    22021 tttcactgct tctgtttcta aaagatcttc tacataagac atatattctt cgtcttcacg
    22081 ccatttttct gtcataatta tcgatctgcc acctttttga atttcatttt cttctgaaat
    22141 tgatctaagt ataccaccag tgttcggatt attctagtcg ccttggataa aatagccaaa
    22201 gcttaatagt tccttaatca taaagaaaaa tgataaagaa caatccctgc cgcaacccca
    22261 acattcaacg attccgcatt tcctttcatc gggatatata cgttctgatc agtcaaagac
    22321 aagatttcct gacttacccc ctgaccttcg ttccccatga ttattgcgaa gttatctgat
    22381 ttttctactt tgtctaaggc gtgtgcttct tcatttaatt ctgtgccata cactgtaaac
    22441 cctttgtctt tcattaaagg aatcacttct gtcaaatctt tttgcaacac aggcaaatga
    22501 taattgcttc cctgcatgct gcgcagcacc tttgtactat aaatatctgc cgttcctttg
    22561 cctaataaaa caccagccat atcaaatgca tctgccgtac ggatcatcgt ccctacattt
    22621 ccaggatctt gtacattatc taataacagc catcctcctg aaaaatctgg cgtgccttca
    22681 gccgttaacg ccatcactgc taagattcct tgtggtgtag gtagatccga tacagcatcc
    22741 atgacctcat ccgaaactag aaaagtagaa atctctttgt ttgcggccag ccagtctccc
    22801 cattctttca tgcctcgctg tgtcacaaaa agttctttta tttctgcatc agacgacgca
    22861 gcttcttcaa tcagatggaa cccttccaaa agatattctt ccttttcttc ccgatatttc
    22921 tttttatgta gtttttttaa ttctttgatt cttggatttt ttgctgattg gatctctttc
    22981 acattcctca cctccccatg attatatcat ggaataggtg cttgagcttg tttcatccta
    23041 aaaaaatcgg tatcattaat ttataaaata ggaaagaaag gtgacgaaca tgcgcaaagt
    23101 taaaatgaac gtccaaggac gcgtccaagg cgtaggattt cgctacatga ccaagatggt
    23161 cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa gatgacggct cagtcacgat
    23221 cgaagcaatc ggcaatgacg acatcatcca gaaatttatt gaagaagtca agaaatctcc
    23281 gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa gacccgatga ttgaggaacg
    23341 aaagaaattt gatgtggtgg gataaggttg tgaaaaagct ggtctgcatc aagcattaag
    23401 gacaggttgt gaaaaatagc tctccatatt tttcaacagg ctggacttaa tgccgtagat
    23461 gcaagctttt gaacaccgtc aataaggttg tgagaaagtc gcttagtttt gagtagtaag
    23521 gagaattttg tgaaaatggc tttccacatt tttgcataaa atttgactta ataccgagaa
    23581 actgactttt gaacaccgtc gataaggttg tttttgagac gctttgcctc aagtattaaa
    23641 gataggtagt aaaaaagctg atctataact tgcaataaat ttgcttaacc catattttcc
    23701 agacagaaca aaaaagtttt tagtttttta aataagaaag aaacacttgg aatcactctt
    23761 ttctttccaa gtatttcttt cttgattttt tgtctttttt tcaaaatttt tcgctacgta
    23821 aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg cgctttttta
    23881 gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat aaatggaaaa
    23941 actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc tgtgtgcgga
    24001 tggattcaaa cggaaaccct gacacttcgg gaatcgttta tcgagtactc gtccatccta
    24061 tgggccaagc gattacatat ctagttgaga atttcaattg gtcttatggc tgggcagtta
    24121 ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact aggtattagt caatcgaaaa
    24181 aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa gcagcccaac
    24241 aaaaattgaa agctgcaaca acacgtgaag aacaaatggc agcacaagca gaaatgcagc
    24301 aagtataccg cgaaaacgga ttgagtatga ccggcggaat cggctgtctg cctctattga
    24361 tccaaatgcc gatcttctct gccttgtact ttacggcacg ttatacagaa ggtattcggg
    24421 aatcatcttt ctacggcatc gatttaggct caccaagtat ggtacttgtt gtcattgcag
    24481 gggtcgccta tctgcttcaa ggatacatct caacgatcgg tattcctgaa gaacagaaaa
    24541 agacgatgcg tacgatgcta atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg
    24601 cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt ctgcaaacat
    24661 tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac
    24721 aaaacccgcc aaaacaagtc gtcactccaa gaaaagacgt gacagaatca gcaaagccag
    24781 ctaacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    24841 gccgcaacgc aggaaagcag cagaagcgga aataaaaaca taggacaaaa aagatgagag
    24901 ctaagccctc atcttttttg ttactcttcc tcttctgcta ttccattaat taattcgttg
    24961 acagagacaa tgctttcaca tcctgcacga acaaactcgg gctctgaacc aatcatttgt
    25021 gagttgataa attcaatgac catcggaaga ttcatcccag cataaagagt aatctgttgt
    25081 ccttccatta ttagtttaga aatgatattt gcaggtgtac cacctaaaag atcgcatagt
    25141 accacaaaat catttaacga atctgtgaca gtgagaaact tttgtttgta tgtttcagtg
    25201 ccatcttctg gtaataaagg taacgtgtag atatcctctt gaggccccat gatcatttct
    25261 gtactttttt tcaattcctc acaaaatttt ccgtgactaa tcaataccaa ttgtctactc
    25321 atttttcgct ctcccttatt tcgaaatcac acccaatgca gataaagcaa cacacacaat
    25381 taatacgata aaaatcgctc gagtaggagt catgtttttc tttcctaata accaataaac
    25441 accaccgacg atcccagcag gtaaaatttt aggtaagatc atatcgatat agttttgcat
    25501 attcaatgtc acatcgccaa tcgttggtgc ccatgaaaat ttaacattga tcattgtcgc
    25561 aactaatgcc cctaccatga atacccctaa taaagtggct gcgtccgtta atgcagttaa
    25621 gcgagattgc atagtagtaa ctaaggaaat tccttctttg tatgcaaatt caagttgctt
    25681 ccaacgaaaa accatgacaa cgatctgagc aaaaatccat ataaacacac ctatcggatt
    25741 tccatcagtt gccatattcg ccgccaaagc cccaaaaatc gttggaatca aagcagcaaa
    25801 tacggaatct ccaatcgctg caaaggaacc catcaatcca gctttcagcc ccgatacggt
    25861 ttcctttgaa gcaattccct ctttttcttc cattgcaaga tcaatcccag taatgattgt
    25921 attaaaaaaa ttagaagtat taaaaaattg cgtatgtgtt ttcatcatct cttttaattc
    25981 tggtgtattg tctccatata tttttcttag ttgtggcaag atagtatata aatagccaga
    26041 accctgcatt ctctcatagt tccaacctaa ttgaaaagtg aacaaacttc ttcgattgat
    26101 ttgcttaaaa tcttcttttg ttaatttgta attagatttc gtcatcttcg atttccccct
    26161 caacagaatg tgatggctca ctagctaatg gttgatggtc tttgttttga tcattcttat
    26221 aactgatcat tgctagtgct agaccaatca atgctacagc aagcatcggc agcgaattga
    26281 aagttgccgg aagatcttta acaacgcttg ccacacttgt tcctaataat tgaacattag
    26341 agaataacgt tgctaataat gccgtgatcg taaatcctaa aataagataa gatagatgtt
    26401 tttttactgg taaatagcgc agaagaatgg caaacccaac agctggtaaa acagctcctg
    26461 ctactgaaag tccatcgcct agccatttta gattcccgtt taaaacagaa acaacatttt
    26521 caactaatcc accaccaaat gctaaagcta aaaatacagg aatcatacga gagagcgacc
    26581 aaggtaaagc tcccattaag aaattgcgtt cgattgcttg ataattcatg tcttctacgt
    26641 gacggtcaat acgatgggca aagtatgtgt tagcgaatct agctaaaata tccaattgga
    26701 tcattaagct tgctacaggc actgcaatag cagcaatcgc ttgttctgga ttcattccaa
    26761 gagaaatcga gaaggcagtt gctaaaatcg tcccagagtt ggcatctatt tttgatgcgc
    26821 ctccaaacgt ccccactcct aaaactgtca actgcatact gccaccaata atcaaacctg
    26881 cgtttaaatc acccattact aaacctgcat ataaccctgc aaacactggc gcactcattg
    26941 aagaatagat ctgtaattca tctaaaattt gataacctgc atataatgtt aataataaga
    27001 tctgccacca ttgaatatcc atggatcatt cctcctattt gattaaaaga ttcataaagt
    27061 cttctgcttt atcgtttggt accatttgag caattaaatg tataccaagc tgatctaatt
    27121 ttttaaatac ttcgatatct gcatctacca cattgattga ttttgtaatg gaccgtgttt
    27181 cattcgtttg tgacatattc cccacattta cttctttgat aggaactcct ttttctacta
    27241 aagataagat tcgatctggc ttttttgcta cgatcaatac tcgttgagag tcatatttgc
    27301 cagctaaaat atttgttgct gctttttcga ttggcaaaat gcttaatttt acccctgctg
    27361 gagtcgctaa cttcaatcca cttttttcaa ttgtgtttgt ggctacctca tcatccacga
    27421 ccatgattcg actaatattc agtttcgttg tccaaagatt tgctacttat ccatgtatca
    27481 aacgtccatc gattcttaca ccaataattg ccatcatgca ttctccttat ctattttttc
    27541 tatttattgt tcaattaaaa ctgtgacatc atcaaatatc ccttctgttt caaagataac
    27601 gttttcattt tttctttctt atatttcatt tttaagctaa gaacaaattc cttcccaact
    27661 aaagaaaacg ctttctttta tatttaagtt catttgattt tcttaacttg aatgtgtttc
    27721 atcaacatcg taactttcat agggtcgatc cagccagttt tctcttgttg cgcgtttgcc
    27781 ataccacaag ccatagaaaa tgcgagaatc tcttcgattg attgcctttt tgataacgca
    27841 tgggccgatc ctgcaagggt cgcgtcacct gaaccaacag gattaacaac tgatatttta
    27901 ggaataatga cttcgtaaaa acactgagca acttttgcca cagcacctct ctctcctaaa
    27961 gaaacaacaa cccatggcac tccttcaaat attggatgag ctaataagga ctgaattttt
    28021 tctaattgaa tcgaagaaca gatacgttgg ccaatcagtt ctcctaattc ttccagattg
    28081 ggcttgataa gtagtggctt ctccttacct tccaatactt caagcaaaaa tttaccagaa
    28141 gtatctagta atatgtcttt ccctttttta tcagctaaag caaccaattg cttatagtaa
    28201 tcaagagggc atccttgggg taaactacct gagatcgtca taatatctga tatttcgagg
    28261 agctgctcga aatgatcggt aaatgtctgt tgttctttct ttgttatgaa ggggcctcgt
    28321 tccaaaagct ctgtttgtct cgaactatgc aggatagcaa tagaatccct tgtctctcct
    28381 ttaattggaa caaactgatg catgatccct aactgatcta gctggttctt tagatattca
    28441 ccgtgctggc cacctattaa accagtagct atcacctctt ctcctaataa atttagaacc
    28501 ctcgccacat tcactccttt tcctcccgct gttttattga ctgtttcaac tcgattgact
    28561 ttgtcgaggt ttaattcttc aattgtatac gcgatatcga tggaaggatt caaggtaact
    28621 gtcgtaatca tttcatcttc acctttttgc tttttgtttt attgaaccaa ggggtagcgg
    28681 tttcatttat tactttattt aaagtctcga tgttttctct cccatttgtt cttaaccaat
    28741 gaatagctcc gtcttttcca ttttcgacgt atgctttgac cccatctttc catgtcgcac
    28801 gtccacacaa tacaccattg aaggtggaac ccgattcatg agcaaaaatc agtgtttgtt
    28861 gaaataattc tgttgaaaca cccccactca aaaaaatgaa tggtaatgga gtcgcttcac
    28921 tttgctcctt aaagaaggca gcagcctctt ctttgctgtg aataaattca cctgtggaat
    28981 agccattcac ataattcata ttgactggta cttctacttt caacacatca actttatatt
    29041 ttggatcaga aaattctttc atcatctcaa taactttgtg gggcttgact ttcgcatact
    29101 ctggagaaga actatcgctt atttgtgcgt cataagaaac taactccaaa tagaagggac
    29161 gattttctcc ttcgcactca ctacctaaac gttcgatgaa cacatgcttc aaatgattga
    29221 tttcgggtgc ttcatcaaca tcgtagtaaa gtaagaattt tactgcatcc gcttgctgtt
    29281 ctttcaatct gagagaagac caatccgcta ataaatcagg tagacgacca ggcgtagttg
    29341 cgtcatagcc ggttttctca taagctaaca ataaccccgt ctcttttgct ctgacttttg
    29401 ctgctggcaa tccatattcc ggatccaata aaatagcaga agcatagggt gtcagctcag
    29461 ctgatactag ttctttgaat tcactgattt ggtcttcact tggctctttt cccaacgatt
    29521 tgatcatttt ttttagagca ccacgctgat cgatcgctaa tgcactaata atcccatctt
    29581 ttgttgatag tctctccatt gccctatttt tttcttcatt catctgtcat ttctccttcg
    29641 gtttcatact tatgaataat gacgcccttg actactcgat tgaccgtgcc tgttggagat
    29701 ggtgtatctg gtttattagc tactttgatt gaagtataga gtgcaatgat ttgtgcagtc
    29761 ataatcatcg ctaatgcaag ataggcatcg ggcaatttac tcaccgagtc ttccaatagc
    29821 acagtcatac ctgagaaatc aatagtaccc ggttgcgcaa tcgcaatgac attggcagtg
    29881 acccgatctt cttttatttc ctctaaaata tctaagtcat aatttcgcgt atatggttga
    29941 ttgctcacaa aacctacaac gagtgtctcg ctatttaaaa aagactttgg tccatgacga
    30001 aaccccatcg aagaatcaaa aattgtagca actttacctg ctgttaattc taaaattttt
    30061 aattgtgctt ctcttgtcgc acctgccaaa cttcctgaac ccagataagc gatccgttca
    30121 aaaaaatcag gtaataactt ttgaatttct tgatctcttg aaagaagatc ttgaccgatt
    30181 ttaactaatt gttcaagata tctttctttt ttctccattg ctgtttgatc aaagatccat
    30241 aaagcactca ataacataca cgtaaaactt cctgtcatag caaaacctgc atcgtttgct
    30301 ctctctggca tcaataacag ataattctgt ttatcatcct gtgcgttttt tgccaacatc
    30361 ccctctttag cacaagtaat cgtcaaatga aacaatgttt caacttgttc atttgcaatt
    30421 tgcacagccg ctacactttc tggactgttg ccactgcgag caaatgaaac taataaagtc
    30481 ggttcatttg gaaacaaaaa ttcttttggt gaagctacaa tatctgtcgt gccaaaactg
    30541 tggaagataa aaaactgatt gtctccatgt tcatttagat agggtaagag cgtatcccca
    30601 atatattgag acgttcctgc acccgtaaaa ataacacgta ttcttcttcc gtttgcttct
    30661 ttttttattt gatcaaaaaa tagagctaaa ttttcttggc tttgcatcaa tctatcgatt
    30721 gtttctttcc agagatctgg ttgttgtttt atttcttgtg tcgtgattgc tgcacctatt
    30781 tgttttaatt tgttcttcga tagctcaaac atgttgttct ccctctcaat cattttcgga
    30841 tatgccgaat tttataatga aactggtccg ctcttgctac actatctgta tattcgataa
    30901 tctcattttt cacgttatag gttatcctcg tcagatgtaa gacgggagcg ccatcagcga
    30961 ttcgtagcac aacagcatct tttttttgca cgatacttgc ataaaactct tcatcagcta
    31021 attgaacttg ttgttcataa tcctgtgaaa acacatcata caacggttta ctttcaagta
    31081 acggcaaatc aagtcccata aatagtttcg ccggtagata agaacgctca accatcaatg
    31141 gctcttcatc tgccaaacgc aatcttttaa gtttaaatac agtttcacct aaatggatgt
    31201 tcaaattctt agcaattctc ttattagctt ccatttgtct aaattctaaa atgaccgtct
    31261 tagggacgcg ccctatttcg cgcatttctt ctgtaaagct atatgctcca gctaagttcg
    31321 atgacttatc agacaaatca gagacatagg taccttttcc atgtcttcgg taaatataac
    31381 cttgattttc caattcctgt aaagccagac gtacggtggt acgactcacc ccatagtgtt
    31441 gacaaagttc tctttcagat aacagcttat catgcggagt catctgtgtt tcgatacgat
    31501 ccttcaataa atcaactagt tgataataca atgattttcc tttatttgag ctctccataa
    31561 aaatcacctc ttctttactg gttataacca caacacaaga atagtgaatt attaatcatt
    31621 tgtcaaaggt tttttagacc aaaaaagcta ttataacagc tctttaaagc tttatatctt
    31681 gaatagacaa agatacctca aaaataccag ttaccaataa aagaacagaa aaaaagacga
    31741 accacataag cgatccgtca aaatggtcta acttatcgag cagcgtgatc cacattaatc
    31801 cctatgatat tcctacaact atcgttttat tacttccaat atcatttaca acaactaaaa
    31861 atagcttatt aatgacaaat gttattatta gttttagata acttttgcag ttttatactt
    31921 tttttctata aaatttgaca aacatttttt taaataacta attatttttt gaatctgtca
    31981 ctcagacaat cctttctctt acttgttaac ataaatagaa gttgctgttt cttctaatag
    32041 tgtacttaat tctttaatat tttgttttcc ttcttctaac atccaactac tcgctttttc
    32101 ttcgccttcg gtaacaaaag gtgctactcc cttcgcccat gttgctcgcc cacataagac
    32161 accattgaat gtcgaacctg attgttttgc aaaacgcaat gtatcttgaa atagtgcagc
    32221 gctgactccc gcacttaaaa aaataaacgg ttgttcagtc actttcgatt gttcatagaa
    32281 atacgcggat gcctctgctt gactataaca agtatcttgc ccatatcctt ccacaaatct
    32341 catattcact ggtacttcta ctttcaatac atccacttga taacgttctt ttgaaaattc
    32401 tcgcatggct tcaatcactt tatgcggttt tacttttgca tagtcaagag aagacggatc
    32461 gccaatagtt gcatcgtaag aaaggagctc taaaaagaaa ggtatgcctt ctgcttcgca
    32521 ttctgaacca atccgttcta taactatttg tttttgcaca ttaatctcta ctggttcatc
    32581 aacgtcatag tacaataaaa atttacaagc atccgcaccc acttctttta accttttcac
    32641 tgaccaacta ttcagtaaat ctggtagtcg gcctggcatt ttcgcatcat agccggtttt
    32701 ttcataagct agcaacaaac cacattcttt ctctcgaagc gctgctgcag gcagaccata
    32761 ttctggatcg agtaaaatag cggaagcata cggagtcagg tactgagaaa ccatctgttt
    32821 aaattggatg atatcttctg atgtagcttc tttatattgg ttgatcattt tcttcaaagc
    32881 accacgttgg tcgatagcta gcgcgctaat aattccagct tcatccgtca atcgcttcaa
    32941 acgttcctgt ttttctatac tcattttctt catatatctt cacctatttc tttagttaat
    33001 attgtagcta tttctatttt tgattttgca gctctcaact gttctgtaac ctcttttcgc
    33061 atcaatgatc gtgcaacgag agacaataat ttcaaatgag tcgttcctgc ttctgaatct
    33121 gggataaata acgcaaaaat aaaatcaact gactgtctat ctaaactgtt ccattcaatt
    33181 ccttttttca gtgtgacgat aatcaaacta ggttcattga ttgtttgaga cttcgcatga
    33241 gggatagcaa aacctgacat tattcctgta gtaccttctt tttctctttc cagaagtttt
    33301 tgatatactt ctttttcgtc atgagcgatt cctaaacaga atgtttgctt tgctaaaaac
    33361 ttaaatgctt cttcttgatt atctaatggc tgttgtaaaa aaatgttctc ttcatgaatg
    33421 attgtcatga taaacctcct gctcttcctt cttcgataac tgtacgattt gttgctttat
    33481 tatccctctt cttgtcttta tcagttactc ttttcttttg gatcaaacct agtatcactc
    33541 caccaacgat cgaaccaata agaatcgctg taacccattg gatttttccc gtcactacag
    33601 gtaacactaa aaaaccgcca tgtggggctg gtacttggac accaaacaga taggtcaaga
    33661 cagcagcaat tgatgagcct aacatcatcg ttggtaatac tcggatcggg tctttcgcag
    33721 caaacggaat agctccttcc gtaatatgag ttgatcctaa aataaaatta accaaacctg
    33781 cattacgatc attagtatca aaatatttac caaacaataa agtagcaaaa ccagtaatca
    33841 acggtggtgc aatgcaagct gctgacgcac cagccataaa agtcgtgttt ccttgtgtca
    33901 aaagagctgt tcctgttaca tatgccgcct tattaaccgg tcctcccata tcaaacgcac
    33961 acatacagcc aacaatcacg ccaagaacaa gtggattcga atattcaaat ccagctaaaa
    34021 agctcatcat tccttcattt atagccttca ttggacctgc cagcaaagac atcacgatgc
    34081 caataaatgc tacaccaata actggatata agacgattgc cttcaagcca tctagttgtt
    34141 taggtaaaat tatcagtagc ttttgcaaaa ataagataaa ataacctgct aagaacccag
    34201 ctaaaatacc tcctaaaaat ccagtaccgc cattcatggc tacgattcca ccaacaaatc
    34261 caacgactaa ccctggacgt ttcgcgatcc cttctgcaat ataagctgct aaaactggaa
    34321 ccatcattcc cattgctgca tcgtcaactg cttttaccat tgccgctgta gcattgtatt
    34381 gtgcatgatc tggatcagaa gaatagattc cccaaacggc aaatgaaata gcaatcaata
    34441 ctccgccagc taccacgaaa ggcaacatat gagagacacc gttcatcaaa tttttataga
    34501 tgctacgacc aaaattagca ccacttatcg cttctggttc taaagtatca gcagcttcat
    34561 tatctgctaa gacagtacct tttcctgcca atgcttcgtc aatcagccgt tgtgcatcct
    34621 tgattcctgt actgacagaa acatcaatga ctcgttttcc cacaaatctt tctgaatgga
    34681 catctttgtc tgcggcaata atgacagcat ctgcctgttt gatttcttca gccgttaatt
    34741 cattctcgat cccaatttgt ccatgggtct ccactttgat cgtgatgcct tttttcctag
    34801 ctgcttgctc taatgcctct tgcgccatgt aggtgtgcgc aattcctgtc ggacaaccgg
    34861 ttgctgcaat tagttgatat tttcccatct ttcgaacctc tcttccttga ttttgatttg
    34921 tttacttaat tcctccacat ccaaaaaatc agtcagtcct ttacgaaaag cagtagagct
    34981 gcctgctgct atgcttttac gtaacgtttc atcaagcgat cgtcgattca tgtatcctgc
    35041 taaaaaagtg cctaacatgg catcacctgc acatgctgta ttgacaactt ttccttttgg
    35101 cgagtttcct cgaatcaccc tgtctttcgt aaaaagaatc gctcctttct caccgaggga
    35161 taacaaaaca ttttctgcgc ctgactctac tagtcgttgt ccataaacaa agtaatcttc
    35221 atctgtaacc atttttcgtc cgaaccaaga agaaagttct tcctcatttg gtttcagcaa
    35281 aaacggtcga tagggtaaac aatccatgac ttcttgatca ctactatcaa taatcaaaca
    35341 aatccgactg atttctatta aaacctttgg agacagccct cttggtaaac tcccagaaac
    35401 acaaagataa tcccctgctt gtaatgctct tatttgattt aaaaaattat gaacagctgt
    35461 ctgcggtatt tcaggtcctt gattcactaa tttgtattct tctcccgttt gattgacttg
    35521 tgtaaataca ttgatacgcg tcataccagg cacttcaata aactctgttt ggatttgttt
    35581 ctgctgtaaa tagtcttcta tatactttcc tgtgaaccca gcagaaaaac ctaatgccgt
    35641 acttgggaaa tctagcatct ttaatactag tgacacattc accccttttc cgtttgcctg
    35701 aatgtcatcc tcttttgtgc ggtttaccat aaaaggatgc atctcatctg tttcaataaa
    35761 tagatcaatt gccaaattca tcgtgcatgt gtagatagac ataaccaacg acctccttga
    35821 ttctattatg aaatacgcga aatagaaaat gttttcttaa tcgtcccaac acaaagaaaa
    35881 cctttacatt tataaactcg tcgtgctttt tttaactaac ttttggtgaa agaaggaaag
    35941 aatatgaatc acaaattaag tgaaacagag cagtacctct ggaactttat cgaacacctc
    36001 atcctagaga tccctaacta ttccatcgtg aaactaagtg aacaagcaaa tgtctcaaca
    36061 gccaccattg tgcggacaat gaaaaagaaa ggctatgaag ggtttacttc gtttaagcat
    36121 catctaaaag aaggagaaaa caagaacatc aatttttcat ttttagataa agtagacaaa
    36181 ggaatccgcc gagccattct aaaaaatgaa caagaagtgg tacgtacaat caatatgttg
    36241 gaaatcggga acattgaaga tgcaatacaa agaatcaaat ttgctgaccg ggtttttgtc
    36301 tttgcccgag gattttcaga aatgattggc caagaaatgc tggtaaaact tcaattaacg
    36361 ggaaaaaatt gtcagatgca tactgatcca gaaattatca aaagcatcag tcggaaacta
    36421 actaagaaag acagcgttat ttttgtttct ttgaacggtg agaaaacaaa taaaaacatc
    36481 aaaaataaga tttatgtctt tatttttcaa ttttttaaat
//
LOCUS       DAZKSR010000012        35818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000012 DAZKSR010000000
VERSION     DAZKSR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.012"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1088
                     /locus_tag="ACG8XG_000574"
     CDS             <1..1088
                     /locus_tag="ACG8XG_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0772515.1"
                     /translation="DVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAADPN
                     KRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAPEAA
                     APKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVTLHD
                     EVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQEIVY
                     KHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRNGTI
                     TISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFDHRI
                     VDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1096..2502
                     /gene="lpdA"
                     /locus_tag="ACG8XG_000575"
     CDS             1096..2502
                     /gene="lpdA"
                     /locus_tag="ACG8XG_000575"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0772516.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2664..2939
                     /locus_tag="ACG8XG_000576"
     CDS             2664..2939
                     /locus_tag="ACG8XG_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0772517.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            2945..3712
                     /locus_tag="ACG8XG_000577"
     CDS             2945..3712
                     /locus_tag="ACG8XG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0772518.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3880..5709
                     /gene="typA"
                     /locus_tag="ACG8XG_000578"
     CDS             3880..5709
                     /gene="typA"
                     /locus_tag="ACG8XG_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0772519.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            5970..7742
                     /gene="recQ"
                     /locus_tag="ACG8XG_000579"
     CDS             5970..7742
                     /gene="recQ"
                     /locus_tag="ACG8XG_000579"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0772520.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7856..8158
                     /locus_tag="ACG8XG_000580"
     CDS             7856..8158
                     /locus_tag="ACG8XG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0772521.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8161..9324
                     /locus_tag="ACG8XG_000581"
     CDS             8161..9324
                     /locus_tag="ACG8XG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0772522.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9350..12778
                     /locus_tag="ACG8XG_000582"
     CDS             9350..12778
                     /locus_tag="ACG8XG_000582"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0772523.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12799..13923
                     /locus_tag="ACG8XG_000583"
     CDS             12799..13923
                     /locus_tag="ACG8XG_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0772524.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13904..14350
                     /locus_tag="ACG8XG_000584"
     CDS             13904..14350
                     /locus_tag="ACG8XG_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0772525.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14334..14672
                     /locus_tag="ACG8XG_000585"
     CDS             14334..14672
                     /locus_tag="ACG8XG_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0772526.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14791..15351
                     /gene="rsmD"
                     /locus_tag="ACG8XG_000586"
     CDS             14791..15351
                     /gene="rsmD"
                     /locus_tag="ACG8XG_000586"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0772527.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15348..15839
                     /gene="coaD"
                     /locus_tag="ACG8XG_000587"
     CDS             15348..15839
                     /gene="coaD"
                     /locus_tag="ACG8XG_000587"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0772528.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15829..16872
                     /locus_tag="ACG8XG_000588"
     CDS             15829..16872
                     /locus_tag="ACG8XG_000588"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0772529.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17031..17759
                     /locus_tag="ACG8XG_000589"
     CDS             17031..17759
                     /locus_tag="ACG8XG_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0772530.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17875..18120
                     /locus_tag="ACG8XG_000590"
     CDS             17875..18120
                     /locus_tag="ACG8XG_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0772531.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18117..18335
                     /locus_tag="ACG8XG_000591"
     CDS             18117..18335
                     /locus_tag="ACG8XG_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0772532.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18471..19010
                     /locus_tag="ACG8XG_000592"
     CDS             18471..19010
                     /locus_tag="ACG8XG_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0772533.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19003..21276
                     /locus_tag="ACG8XG_000593"
     CDS             19003..21276
                     /locus_tag="ACG8XG_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0772534.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21314..22360
                     /gene="holA"
                     /locus_tag="ACG8XG_000594"
     CDS             21314..22360
                     /gene="holA"
                     /locus_tag="ACG8XG_000594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0772535.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22449..22703)
                     /gene="rpsT"
                     /locus_tag="ACG8XG_000595"
     CDS             complement(22449..22703)
                     /gene="rpsT"
                     /locus_tag="ACG8XG_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0772536.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23103..23372)
                     /locus_tag="ACG8XG_000596"
     CDS             complement(23103..23372)
                     /locus_tag="ACG8XG_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772537.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23634..23762)
                     /locus_tag="ACG8XG_000597"
     CDS             complement(23634..23762)
                     /locus_tag="ACG8XG_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772538.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23842..24297)
                     /locus_tag="ACG8XG_000598"
     CDS             complement(23842..24297)
                     /locus_tag="ACG8XG_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0772539.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24435..26363)
                     /locus_tag="ACG8XG_000599"
     CDS             complement(24435..26363)
                     /locus_tag="ACG8XG_000599"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0772540.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26560..27393)
                     /locus_tag="ACG8XG_000600"
     CDS             complement(26560..27393)
                     /locus_tag="ACG8XG_000600"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0772541.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27585..28289)
                     /locus_tag="ACG8XG_000601"
     CDS             complement(27585..28289)
                     /locus_tag="ACG8XG_000601"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0772542.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28312..28938)
                     /locus_tag="ACG8XG_000602"
     CDS             complement(28312..28938)
                     /locus_tag="ACG8XG_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0772543.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29264..31639
                     /locus_tag="ACG8XG_000603"
     CDS             29264..31639
                     /locus_tag="ACG8XG_000603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0772544.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31663..32154)
                     /locus_tag="ACG8XG_000604"
     CDS             complement(31663..32154)
                     /locus_tag="ACG8XG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0772545.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32341..33261
                     /locus_tag="ACG8XG_000605"
     CDS             32341..33261
                     /locus_tag="ACG8XG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0772546.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33415..33759
                     /locus_tag="ACG8XG_000606"
     CDS             33415..33759
                     /locus_tag="ACG8XG_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0772547.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(33949..34449)
                     /locus_tag="ACG8XG_000607"
     CDS             complement(33949..34449)
                     /locus_tag="ACG8XG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772548.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            34977..35060
                     /locus_tag="ACG8XG_000608"
     tRNA            34977..35060
                     /locus_tag="ACG8XG_000608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35011..35013,aa:Leu,seq:cag)
     gene            complement(35384..>35818)
                     /locus_tag="ACG8XG_000609"
     CDS             complement(35384..>35818)
                     /locus_tag="ACG8XG_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0772549.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11849 a         6248 c         7389 g        10332 t
ORIGIN      
        1 gggatgtatt agtagaaatc gatgctcctg gacacaatag tgcaccgtca actagtgcgc
       61 catcagctga agcaccaaaa gaaaaagtgg aaacatcagg ttcagcaagt gtcgttgaag
      121 ctgccgatcc aaataaacgt gtattggcta tgccatctgt tcgtcaattt gcccgtgaaa
      181 aagatgtaga tatctcacaa gtaactgcaa ctggaaaagg tggcagagta acaaaagagg
      241 acatcgaaaa cttcttggca ggtggtccaa gcagtgcacc agcgaaatca gaagcgccag
      301 aagcggcagc accgaaagaa gcagctcctg cagcagaaag caaaccagct gcaccagcaa
      361 aaccattcaa gtccaactta ggagacttgg aagaacgtgt agcaatgacg ccaacacgta
      421 aagctatcgc taaagcaatg gtcaacagta agcatacagc tccacacgtg acattgcatg
      481 atgaagtaga agtgtctaaa ctatgggata atcggaaacg atttaaagaa gttgctgctg
      541 caaatggtac gaaacttact ttcttgccat atgtcgtgaa agctttaaca gcgactgtta
      601 agaaataccc agtattgaat gcatctatcg acgatgcaaa ccaagaaatc gtttataaac
      661 attactacaa tatcggtatt gcaactgata cggatcacgg actatatgta ccaaatgtga
      721 aagatgcaga ccgcaaagga atgtttgcta ttgcagacga aatcaatgag aaagcaaaat
      781 tagctcatga cggcaaacta tctgcagaag atatgcgcaa tggaacaatt acgatcagca
      841 atatcggatc tgtaggtggc ggttggttta caccagtcat caactatcca gaagtagcta
      901 ttttaggtgt aggaacgatt gcacaacaac caatcgtaaa tgctgaaggc gaaatcgttg
      961 tcggtcgtgt gatgaaactg tcattaagtt tcgatcaccg tatcgtagat ggagcaactg
     1021 cgcaacaagc aatgaataat atcaaacgtc ttttagctga tccagagcta ttaatgatgg
     1081 aaggatgata aacaaatggt agttggagat tttgctgtag aattagatac agttgtaatt
     1141 ggtgcgggac ctggtggcta tgtagctgcc atccgcgctg ctgaaatggg acaaaaagta
     1201 gcaatcattg aaagagagta tattggcggt gtttgtttga atgtcggctg tattccttca
     1261 aaagcactga tagctgcagg acaccattat caagagtcac tagattctga aatgtttgga
     1321 gtaactagtg aaaacgtcaa actagatttt gcaaaaacac aagaatggaa agaaaacaaa
     1381 gttgtccata cgttgacttc tggtgttggt ttcctattaa aaaaacataa ggttgaaaca
     1441 atcgaaggag aagcgttctt cgtagatgat cacacactac gcgtcattca tccagattca
     1501 gctcaaactt attcgttcaa taacgcaatc atcgcaacag gttcccgtcc aatcgagatt
     1561 ccaggattta aattcggagg acgtgtgtta gattctactg gcggattagc attgaaagaa
     1621 gtacctaaga aatttgtgat catcggtggc ggtgtgattg gtgccgaatt gggcggagcg
     1681 tacgcaaact taggagcaga agtaacgatt cttgaaggct caccacaaat tttaccaact
     1741 tacgaaaaag atctagtaaa attagtagaa gatgatttca agaaaaaagg cgtaacagtc
     1801 gtaacgaatg caatggcgaa agaagcagtc gataacggtg acagcgtgac agtgaaatat
     1861 gcagtggacg ggaaagaaga atccgttact gctgattatg tgatggtcac tgtaggtcgt
     1921 cgtccgaata cagatgatat gggcttagaa caagcgggtg tcgaagtagg agaacgagga
     1981 ttgatcacgg tggataaaca aggacgtacg aacgtaccta acatctatgc catcggtgat
     2041 atcgttccag gggcggcatt agcacacaaa gccagttatg aagcaaaaat cgctgctgaa
     2101 gcaatctctg gcaaaaaagt agctgtagat tataaagcaa tgccggcagt agccttcact
     2161 gatcctgaat tggcttctgt aggtatgacg atcaaagaag caaaagatgc tggtatcgaa
     2221 gcaaaagcat ataaattccc attttcaggg aacggtcggg cattatcgct tggtaaaaca
     2281 gaaggattca tccgtctagt tacgacgatc gaagataacg tgcttattgg cgcgcaaatc
     2341 ggtggagtcg gcgcaagtga tatggtctct gaattagctt tagcaatcga atcaggaatg
     2401 aatgctgaag atatcgcctt gacgattcat cctcatccat ctcttggcga aatcgtcatg
     2461 gatgcttcgg aattagcatt aggtttacca atccatattt gatttgaata atacaggcga
     2521 aacgcaactg aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt
     2581 aaacttaaaa atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg
     2641 aatatgaaat aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga
     2701 caacagacga aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa
     2761 aagggataaa caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat
     2821 ccattggtga ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat
     2881 atcgtacagt gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag
     2941 ataaatggaa cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat
     3001 caaatcttct caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac
     3061 aaatgtcgat cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga
     3121 ggcgaaaata ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt
     3181 ctttatcatc gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg
     3241 tattatgctc gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat
     3301 gagaaatgaa cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt
     3361 gcctagacca gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt
     3421 cggtcataat aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta
     3481 cggatgcgct ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc
     3541 aaaccttagc ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt
     3601 aacaagcggt ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg
     3661 gacttcttgt gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg
     3721 tagaaatcat tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga
     3781 aaacatttta ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg
     3841 ccctgtcata aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc
     3901 cgtaacgtcg caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta
     3961 ttaaaacaat cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca
     4021 aatgcgctag aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat
     4081 aaaggtattc gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta
     4141 gaacggatca tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca
     4201 atgcctcaaa cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt
     4261 gtcgtaaaca agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt
     4321 gaactattca tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca
     4381 tctgcactaa atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg
     4441 gcacctattt ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa
     4501 ccattacaat tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc
     4561 ggacgtgtat ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac
     4621 ggaacagtta agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta
     4681 gagattcaag aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt
     4741 gtcggtgaaa cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac
     4801 gaaccaacat tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt
     4861 aaattcgtaa ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta
     4921 tccttacgag ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa
     4981 ttgcatttgt ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct
     5041 cgtccagaag ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg
     5101 caaatcgata caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa
     5161 ggtgaaatgc aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca
     5221 cctgcccgcg gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga
     5281 atcatgaacc atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt
     5341 caccaagggg cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt
     5401 atcgaagaac gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt
     5461 atcggggaaa acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg
     5521 acaaacgtac ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg
     5581 acgttggaag aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa
     5641 agcattcgct tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag
     5701 aaaaaataat ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt
     5761 tctttttttg tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca
     5821 gaaaatacgc cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg
     5881 tttcataata agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa
     5941 gtaaaatggc aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct
     6001 atgatgagtt tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg
     6061 tattaggaat catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac
     6121 tattagatgg gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg
     6181 acgcggcgaa tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg
     6241 aaacagctag acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc
     6301 ctgaacgttt tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga
     6361 tcgctattga cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt
     6421 tacagatggc gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag
     6481 ctacagcgac tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca
     6541 accatattca gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa
     6601 aaaaagaaca atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt
     6661 atgcagcaac gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt
     6721 ccgtgggtag atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct
     6781 ttttatacga tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata
     6841 aaagcaatgt ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc
     6901 aggaagctgg tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc
     6961 ctcaagatat ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa
     7021 agcaaaaaga atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc
     7081 ttcagcaata catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta
     7141 actgtttaga tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt
     7201 gcttaaaacg tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat
     7261 cgaaagaaca aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga
     7321 aaaaccaatc gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc
     7381 ttctgaccat caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat
     7441 tgaaaggaca agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg
     7501 aagaaaccga tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag
     7561 caggcgttcc cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac
     7621 gtccatcaag cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata
     7681 aatatggcga agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt
     7741 agaaagaacg aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc
     7801 cttatgaaaa tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga
     7861 ttctttgtcg aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg
     7921 attaatccga aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt
     7981 ggtagataca cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt
     8041 aatcggaaag aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt
     8101 atatagtgag gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac
     8161 ttgcctaaga aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta
     8221 attttatgtg tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa
     8281 aacggacaaa atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg
     8341 attgctatcg cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc
     8401 atggcagcca tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag
     8461 atcaatgggg cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac
     8521 ctaaaaatca tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta
     8581 caaaaagatt ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca
     8641 ttagtcgcta ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt
     8701 ctgcttttag ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt
     8761 tgtctaagta cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga
     8821 cgtttacagt atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta
     8881 aataaagggc atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc
     8941 aggggattag gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt
     9001 atttatgcaa tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg
     9061 ctattcttta tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat
     9121 tcgcttttgt gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga
     9181 ggaattactg gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc
     9241 tccagtttgc tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa
     9301 aaacgcaaga gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt
     9361 tttagttgct aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg
     9421 aattaaaact gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc
     9481 ggatgaagca tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga
     9541 agggatcatt tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct
     9601 tttgtcggaa aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg
     9661 acccaaactg catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat
     9721 cgaagcgggg attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc
     9781 cttggagttt gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg
     9841 cggacgcggc atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc
     9901 gaaaagcgaa gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc
     9961 taatcctaag catatcgaag tacagattct cggggatacg catggaaacg tcatccatct
    10021 ttttgaaaga gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg
    10081 tgtctctatg aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa
    10141 atatgtcggg tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta
    10201 ctttatcgaa gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga
    10261 tattgatatc gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat
    10321 cggattgcct cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac
    10381 tacagaagac ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc
    10441 acctggtggt tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac
    10501 accgtatttt gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc
    10561 cattcagaaa atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat
    10621 tccatttatg ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac
    10681 cttcattgat agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa
    10741 aacgatgaaa tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga
    10801 aaaaccattt tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt
    10861 aacggctaag aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg
    10921 acaggaaaat ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc
    10981 aactcgtgtt cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc
    11041 tgaactcttt tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt
    11101 gaatgaagat ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct
    11161 gcaaatgctt ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt
    11221 ggtggaattt gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag
    11281 tttgaactgg actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat
    11341 cgcagaggca gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa
    11401 tgttcagtat tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc
    11461 aatcaaagat atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt
    11521 gaaagaaacg acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat
    11581 catcacttat tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc
    11641 aatgagtggg aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg
    11701 tccacggctg ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga
    11761 tgtgcgcatg tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata
    11821 tatgcatgaa atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg
    11881 attaggtcat cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg
    11941 cgatatcgtt aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt
    12001 ccaaaatgat cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga
    12061 atctgttgtg actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa
    12121 attgcaaaag attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc
    12181 tgaatcagtc gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc
    12241 aaaacaagac gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa
    12301 agcatataat caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat
    12361 acgtttgggt gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt
    12421 agatgaaatc ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg
    12481 acaaagacgt gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt
    12541 gaaagctgaa ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga
    12601 tgtattagtg aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc
    12661 gatgaaaatg gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt
    12721 gtcttcagga gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata
    12781 agcgaaaggt ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt
    12841 attgtggcat tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac
    12901 tctgatcaac aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact
    12961 ggtttttcta cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc
    13021 tatgatcgat taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt
    13081 catctcacat ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa
    13141 acagggcctg ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa
    13201 atcactacta tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa
    13261 ctaatggaag aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct
    13321 attttatttt ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat
    13381 agtcttttga aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca
    13441 gataaatcgg ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta
    13501 ttaaatatgc ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa
    13561 aaccacagtc agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag
    13621 gaacgtatgg ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca
    13681 ctgacgaatg atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg
    13741 ctatttattc atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct
    13801 tacaaccatg accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa
    13861 gaaaatatgt tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt
    13921 taacgaagag ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa
    13981 gagtaaaacg atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt
    14041 ttgccagttg aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata
    14101 tggtgagtat gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca
    14161 gcttgatatg gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct
    14221 gttggatcaa attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc
    14281 aggtagcccg ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat
    14341 tagacaatag tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct
    14401 taaaacaatt aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga
    14461 aatatgtcgt gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta
    14521 aattacattt tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg
    14581 ccgaaaaaat aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa
    14641 atacagaaat ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa
    14701 aaaataactt ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta
    14761 gtaccagtac catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg
    14821 cgccgtttaa aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa
    14881 tccatattca atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca
    14941 ggaagcggtg gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta
    15001 gacaaaaatt accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca
    15061 gagaaattta ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag
    15121 aaacaacaat tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat
    15181 caaatccata aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa
    15241 acggataaaa cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag
    15301 atttatggca taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat
    15361 tattcccagg aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg
    15421 ctaaattatt cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt
    15481 ttcccccaga agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa
    15541 aagtcatgca tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg
    15601 cgttgatcag aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga
    15661 tgaatcatca tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata
    15721 gtcacgtgag ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct
    15781 acttaccgcc agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa
    15841 caatggttga aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta
    15901 gttcccttgc cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt
    15961 acggtcaatg ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc
    16021 aggcaagcta caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc
    16081 agtgaagaag aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat
    16141 tatatggatt cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct
    16201 tataaaatgg aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt
    16261 ggcaagcttt cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca
    16321 gaagcattta tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat
    16381 ttgcataatg gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa
    16441 aaggcaggaa taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt
    16501 cgtattaatt ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc
    16561 tatgaacaag tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg
    16621 atcaacagtg aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg
    16681 gataaagctg gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag
    16741 aaagaatatc ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg
    16801 aattcctcaa tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag
    16861 aaaatgaaat gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc
    16921 tctttttcgt caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt
    16981 agatgttgaa gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag
    17041 taatgaatga tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca
    17101 agaaaatact agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt
    17161 gtatcgcaag tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg
    17221 ctgcaaaaga aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa
    17281 acgatgtaga agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga
    17341 aggagcctgg tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg
    17401 caggtggatt attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag
    17461 atcagcaagt cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt
    17521 tggcatcctc ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca
    17581 atacagccac tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag
    17641 aaatcattcg atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga
    17701 tttctggtat cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga
    17761 atgattcaaa atcgactggc acgagcaata aatggacgct aatggatact aagaataatc
    17821 caattctaag agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt
    17881 gttcgcaaag aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct
    17941 gggattcata ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc
    18001 tttgccaaag atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat
    18061 gatctagcag agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga
    18121 tagttcagct gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag
    18181 tcgatgagac aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc
    18241 tcacagaagt agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga
    18301 acaaatggat tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag
    18361 tgaagggtag gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt
    18421 aagcaatgag tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa
    18481 aatcttcaaa agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac
    18541 gcattccttg ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt
    18601 gtacacgttt agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct
    18661 ataatggagc agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg
    18721 gacactgctt gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg
    18781 gtatcccgac ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa
    18841 aggctttgtt gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc
    18901 cttatgccca agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact
    18961 cacagcattt ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa
    19021 aggcatgttc gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg
    19081 tattacactg acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg
    19141 ctaaaagaag gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt
    19201 tcttataggc tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact
    19261 gtatttacag atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca
    19321 gagaataaac aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca
    19381 tggcttaact tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca
    19441 gcaaaaaagc ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt
    19501 tctggacggt tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac
    19561 tggcttcaga gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa
    19621 acaagtgtat atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca
    19681 gaagaaattt ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc
    19741 caattttatt tagggtggct gtattatttg tttcgacgta tcggatggcc actacacgtc
    19801 agtattattc cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt
    19861 tctgttttgc gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag
    19921 cattattcca ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct
    19981 cgatttcttt ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg
    20041 atttcttttg atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc
    20101 acgatgccgt tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca
    20161 ttactgattg caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc
    20221 ttcaatcgct ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt
    20281 gagcgaataa tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg
    20341 ctatctggga tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct
    20401 gtgaaacact tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta
    20461 cgcttttctt caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa
    20521 agtccttttc gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga
    20581 gaaaagtggg cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta
    20641 aaaggtcagg ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat
    20701 gtgggagaat tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg
    20761 ggcgctggac gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact
    20821 gagatcattg aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg
    20881 actccttttg aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat
    20941 aacaataggc gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt
    21001 ttgatttatc ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg
    21061 tcaagtaaca cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc
    21121 gtaaataatc gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag
    21181 ataaaaacat ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt
    21241 catcctcatg ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg
    21301 ataatttttg aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa
    21361 agaaaaactt gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt
    21421 tcggacagaa attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag
    21481 ttttgacatg gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt
    21541 tttcggagat caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt
    21601 aaacaatgga atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga
    21661 atcgactgta ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac
    21721 gaaacaattg aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt
    21781 acgtcaatat ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat
    21841 tgatttattc ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa
    21901 attggtcctt tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt
    21961 tcctaaaacg ttagaacata atatttttga catgacccaa tatattttaa aagggaagac
    22021 ggaacaagct ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat
    22081 taatgcgatt ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat
    22141 tggttatcag caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact
    22201 tgccatgcag gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt
    22261 agttgagatg gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact
    22321 attcgttttg cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag
    22381 catgcgggaa ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc
    22441 cgattctttt atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt
    22501 ttgtggatta gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac
    22561 gcatctacgt tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc
    22621 atagcgcttg tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg
    22681 attgcagatt caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt
    22741 taccgcataa ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca
    22801 tgacaagttt ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag
    22861 tacaagctga aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact
    22921 agatttttat ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta
    22981 tataacaaag agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt
    23041 ctttgttttt ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa
    23101 ggctattctg gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt
    23161 tttatgacca actggttccg acggttccat ttgccactta cttccagcac atatttattt
    23221 aggggcaaac gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca
    23281 cggtcctcaa gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac
    23341 gtatcaatta aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac
    23401 ctgaaaacag gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt
    23461 tcttttgtcc actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag
    23521 agcatcgaat tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt
    23581 aaaaaaagcc cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa
    23641 gcagtatcaa acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc
    23701 aaaagtaccg ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac
    23761 attgtatcac ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc
    23821 ctaaaataga caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt
    23881 gataccagaa gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat
    23941 cgtgccgaaa acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt
    24001 ggaagataag acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt
    24061 aaaggcttct aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc
    24121 cgctgtgatg atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga
    24181 acgggaggct ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg
    24241 atttttagct aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa
    24301 atatattcct cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag
    24361 gaattcatcc ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata
    24421 aaaaaacact ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta
    24481 catttttttg aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga
    24541 ataaccaata ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc
    24601 aggaagttag aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg
    24661 atgatccgcc gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt
    24721 ttcattaaga cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca
    24781 cctaaagaag cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt
    24841 acttggtaaa gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt
    24901 gcatagtaat gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg
    24961 ccggttatca tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta
    25021 tttgcttttg gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga
    25081 taaatcacgg ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg
    25141 attatcatcg tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag
    25201 gtagtttgta atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt
    25261 attgtgcaat cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc
    25321 atagcaacaa gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca
    25381 actggttttc ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa
    25441 agcttttcta aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga
    25501 gcagctagag aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat
    25561 aataccattc ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga
    25621 aggatcatta gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg
    25681 ataaatgaag ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta
    25741 gcaaagacag tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc
    25801 agcgttcctg catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt
    25861 aatgcagact catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct
    25921 gctcctttag cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc
    25981 atttgtccat tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta
    26041 atttcttttt gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg
    26101 aatgtcaaca ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa
    26161 gccatcaata aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat
    26221 gtttgtttat atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga
    26281 gagaatcggt cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca
    26341 aaagcttttt tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata
    26401 ataaaaatgt aattgataat aataacagct aaatgagaac gataatggtt atcatttggt
    26461 gattaagctt gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac
    26521 cagaacattt ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata
    26581 accaatcaaa atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt
    26641 cttcaaatag cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt
    26701 tcctacaaga agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt
    26761 gacgatcttc aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc
    26821 tgtagcgaca aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg
    26881 ggatgttccg ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta
    26941 cgtaaagtca ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat
    27001 aataaaggca ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt
    27061 tgcttctaac caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat
    27121 cactttaaac caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa
    27181 tgggtttaac ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac
    27241 gacattgaac atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag
    27301 gatcaaatga ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag
    27361 aatcactgct ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac
    27421 atcttatagt ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa
    27481 cttgagaaat aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag
    27541 actttgcctt ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg
    27601 cgactatgtg ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat
    27661 cgaccgtttg cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc
    27721 tgtttaggat cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa
    27781 acatttccac caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca
    27841 cggtctcgca atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca
    27901 ttagcaattt gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc
    27961 aatacacgag ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa
    28021 caccattctc taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct
    28081 cctagaataa tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa
    28141 aagtcgacat tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg
    28201 aggggtgtat cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt
    28261 cgtacgatat aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt
    28321 gaaaaaagga tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata
    28381 gactgcaaat tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc
    28441 taaattccct gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga
    28501 aacgacaaaa tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac
    28561 gacgcgaaaa gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg
    28621 cgagacaata acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg
    28681 atcacctagc catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg
    28741 taaaggttcc actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg
    28801 ctgttggttt gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat
    28861 gatcaaaagt gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt
    28921 taatctttcg atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag
    28981 tataacgcat tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa
    29041 ttatcagact atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata
    29101 gcattttaat atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat
    29161 atccctgcct aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa
    29221 ctaggttttt tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa
    29281 aatatctgtg cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa
    29341 aaaaagtaac catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc
    29401 gggagaaaac tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta
    29461 cctgatcgtt gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga
    29521 tgaaatcaaa acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt
    29581 ctttcatcaa gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat
    29641 aaatctgcaa ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca
    29701 tcagacaaga gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg
    29761 gcttgttttt caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt
    29821 cccccatgat gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat
    29881 ccgtacgaaa cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac
    29941 gttattcccc gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac
    30001 ggctaaaaca ataactagac aagtagctga agatgctatg acagcattaa aagatacagg
    30061 agctgaagta aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac
    30121 gacctgtaat ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg
    30181 tttatgggat cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc
    30241 aagaaagcat acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt
    30301 aatcattggg gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga
    30361 agatgaaaaa aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg
    30421 ttcccgagag atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc
    30481 tataccaaag gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga
    30541 ttcgatccga gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc
    30601 tgaatcttta gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga
    30661 atttcctgag cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt
    30721 tttgaaggtc agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg
    30781 cgatctgata gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga
    30841 taaaggaaaa agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga
    30901 aacactgggg ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa
    30961 ccagcaagtt ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct
    31021 ggcaaaatta gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt
    31081 tttctttcct tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc
    31141 cgacagcaat gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt
    31201 aacaaaattt caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat
    31261 tttttcagaa ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt
    31321 aggtttgccg cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga
    31381 acaccttgga tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg
    31441 tggtcgagtg attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt
    31501 ttctagtttt tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg
    31561 aagtgttggg gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa
    31621 tcaaaaaaac gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa
    31681 ttgcaactcg attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc
    31741 acggttctta cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt
    31801 ttcatttttg atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa
    31861 atcggcagtt tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt
    31921 tttgattggc acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat
    31981 tttgaaatca tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt
    32041 agaatccttg aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt
    32101 tacttcttct gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac
    32161 cctcctgata tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga
    32221 ttcttacctg tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat
    32281 aaactgttat tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt
    32341 atgactgcgt atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga
    32401 ttgaccgctg cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg
    32461 atgaatggga tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata
    32521 gacacaggaa catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa
    32581 gtaggaaaac gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg
    32641 atgctgcttc cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt
    32701 gttttattag gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg
    32761 gatatcatct ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg
    32821 attctaaatg gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg
    32881 tatacgatta tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat
    32941 caaaaagtaa cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca
    33001 aaacggatgg tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa
    33061 gggaacatga tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt
    33121 catgaaatcg acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg
    33181 tttgcaaatg aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa
    33241 aatcgttcac aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca
    33301 tctaataaga acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca
    33361 agcagctaaa aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc
    33421 gaagcaaacc aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct
    33481 gccttggaaa tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt
    33541 tatgatatag aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact
    33601 atgggctgtc cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg
    33661 ccagaagtaa cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag
    33721 atgagccgtt atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat
    33781 gagaagcaaa taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa
    33841 ggtttctttg tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc
    33901 tattgataaa ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt
    33961 gattttcgta tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg
    34021 tgctttaaga ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat
    34081 tttttttgcc tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata
    34141 attaggggag atgccgtaac caatatggcc agcgccgttt cttaatgttt cgttcagttt
    34201 atggcgcaaa ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt
    34261 tgcgggaacg taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac
    34321 ataatctaaa aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt
    34381 tggtccaaag ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat
    34441 cagtttcatc gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact
    34501 attgttgtaa atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc
    34561 gaacaataca aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt
    34621 atgatctcta gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag
    34681 taatcaacag aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag
    34741 aggaaaaaag aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa
    34801 cattgaaatc gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga
    34861 gttgctcgga tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag
    34921 aacaaaaata tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg
    34981 ctatggcgga attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag
    35041 ttcgagtctt cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga
    35101 tgagaaagaa aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc
    35161 agattcaaat aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat
    35221 tgattcaaat agtagattta tagattctga tacaagaaat actggcaaac gatagttaag
    35281 gaataaagac taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct
    35341 cttactatat tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg
    35401 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
    35461 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
    35521 aacggccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
    35581 tccattcttt tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag
    35641 caggttgtgt ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa
    35701 caggttgttc agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag
    35761 gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAZKSR010000013        35366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000013 DAZKSR010000000
VERSION     DAZKSR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.013"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACG8XG_000610"
     CDS             <1..420
                     /locus_tag="ACG8XG_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0772550.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACG8XG_000611"
     CDS             complement(504..1226)
                     /locus_tag="ACG8XG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0772551.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACG8XG_000612"
     CDS             complement(1216..1953)
                     /locus_tag="ACG8XG_000612"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772552.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="ACG8XG_000613"
     CDS             complement(1965..2957)
                     /locus_tag="ACG8XG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0772553.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="ACG8XG_000614"
     CDS             complement(2972..3715)
                     /locus_tag="ACG8XG_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772554.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="ACG8XG_000615"
     CDS             complement(3956..5278)
                     /locus_tag="ACG8XG_000615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0772555.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="ACG8XG_000616"
     CDS             complement(5265..6230)
                     /locus_tag="ACG8XG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772556.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="ACG8XG_000617"
     CDS             complement(6227..7765)
                     /locus_tag="ACG8XG_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0772557.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="ACG8XG_000618"
     CDS             complement(8181..8468)
                     /locus_tag="ACG8XG_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772558.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="ACG8XG_000619"
     CDS             8602..9270
                     /locus_tag="ACG8XG_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0772559.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="ACG8XG_000620"
     CDS             complement(9561..10301)
                     /locus_tag="ACG8XG_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772560.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="ACG8XG_000621"
     CDS             complement(10431..11378)
                     /locus_tag="ACG8XG_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772561.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="ACG8XG_000622"
     CDS             complement(11507..12271)
                     /locus_tag="ACG8XG_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0772562.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="ACG8XG_000623"
     CDS             complement(12275..12664)
                     /locus_tag="ACG8XG_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772563.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="ACG8XG_000624"
     CDS             12799..13341
                     /locus_tag="ACG8XG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0772564.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="ACG8XG_000625"
     CDS             13489..14121
                     /locus_tag="ACG8XG_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772565.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="ACG8XG_000626"
     CDS             complement(14255..14380)
                     /locus_tag="ACG8XG_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0772566.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="ACG8XG_000627"
     CDS             complement(14514..15407)
                     /locus_tag="ACG8XG_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0772567.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="ACG8XG_000628"
     CDS             complement(15589..16515)
                     /locus_tag="ACG8XG_000628"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0772568.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG8XG_000629"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG8XG_000629"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0772569.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG8XG_000630"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG8XG_000630"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0772570.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG8XG_000631"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG8XG_000631"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0772571.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG8XG_000632"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG8XG_000632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0772572.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="ACG8XG_000633"
     CDS             complement(24786..25220)
                     /locus_tag="ACG8XG_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0772573.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="ACG8XG_000634"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="ACG8XG_000634"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0772574.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="ACG8XG_000635"
     CDS             complement(26117..26992)
                     /locus_tag="ACG8XG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0772575.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG8XG_000636"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG8XG_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0772576.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG8XG_000637"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG8XG_000637"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0772577.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG8XG_000638"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG8XG_000638"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0772578.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG8XG_000639"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG8XG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0772579.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG8XG_000640"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG8XG_000640"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0772580.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG8XG_000641"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG8XG_000641"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0772581.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..>35366)
                     /gene="dprA"
                     /locus_tag="ACG8XG_000642"
     CDS             complement(34530..>35366)
                     /gene="dprA"
                     /locus_tag="ACG8XG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0772582.1"
                     /translation="LLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIKIGKITKH
                     RELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGDLSLLSCN
                     MLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISHGGRTIAV
                     IGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAALSKGTCVF
                     EAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSGEDILAEF
                     KE"
BASE COUNT         9657 a         7493 c         6073 g        12143 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataa
//
LOCUS       DAZKSR010000014        34980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000014 DAZKSR010000000
VERSION     DAZKSR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.014"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..390
                     /locus_tag="ACG8XG_000643"
     CDS             <1..390
                     /locus_tag="ACG8XG_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0772583.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            complement(506..2059)
                     /locus_tag="ACG8XG_000644"
     CDS             complement(506..2059)
                     /locus_tag="ACG8XG_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772584.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            2207..3376
                     /locus_tag="ACG8XG_000645"
     CDS             2207..3376
                     /locus_tag="ACG8XG_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0772585.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3502..4581)
                     /locus_tag="ACG8XG_000646"
     CDS             complement(3502..4581)
                     /locus_tag="ACG8XG_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0772586.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4690..5130)
                     /locus_tag="ACG8XG_000647"
     CDS             complement(4690..5130)
                     /locus_tag="ACG8XG_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0772587.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(5187..5597)
                     /locus_tag="ACG8XG_000648"
     CDS             complement(5187..5597)
                     /locus_tag="ACG8XG_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0772588.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5778..6212)
                     /locus_tag="ACG8XG_000649"
     CDS             complement(5778..6212)
                     /locus_tag="ACG8XG_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0772589.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(6312..7160)
                     /locus_tag="ACG8XG_000650"
     CDS             complement(6312..7160)
                     /locus_tag="ACG8XG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0772590.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            7549..9315
                     /locus_tag="ACG8XG_000651"
     CDS             7549..9315
                     /locus_tag="ACG8XG_000651"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0772591.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            9417..10676
                     /locus_tag="ACG8XG_000652"
     CDS             9417..10676
                     /locus_tag="ACG8XG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0772592.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10823..12124
                     /locus_tag="ACG8XG_000653"
     CDS             10823..12124
                     /locus_tag="ACG8XG_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772593.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            12127..12978
                     /locus_tag="ACG8XG_000654"
     CDS             12127..12978
                     /locus_tag="ACG8XG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0772594.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12991..13806
                     /locus_tag="ACG8XG_000655"
     CDS             12991..13806
                     /locus_tag="ACG8XG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0772595.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            13957..15123
                     /locus_tag="ACG8XG_000656"
     CDS             13957..15123
                     /locus_tag="ACG8XG_000656"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0772596.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            15353..16000
                     /locus_tag="ACG8XG_000657"
     CDS             15353..16000
                     /locus_tag="ACG8XG_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0772597.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            16084..17028
                     /locus_tag="ACG8XG_000658"
     CDS             16084..17028
                     /locus_tag="ACG8XG_000658"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0772598.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            17163..18614
                     /locus_tag="ACG8XG_000659"
     CDS             17163..18614
                     /locus_tag="ACG8XG_000659"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0772599.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            18635..19471
                     /locus_tag="ACG8XG_000660"
     CDS             18635..19471
                     /locus_tag="ACG8XG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0772600.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            19471..20130
                     /locus_tag="ACG8XG_000661"
     CDS             19471..20130
                     /locus_tag="ACG8XG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0772601.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            20256..20945
                     /locus_tag="ACG8XG_000662"
     CDS             20256..20945
                     /locus_tag="ACG8XG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772602.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            20951..21907
                     /locus_tag="ACG8XG_000663"
     CDS             20951..21907
                     /locus_tag="ACG8XG_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0772603.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            21951..22463
                     /locus_tag="ACG8XG_000664"
     CDS             21951..22463
                     /locus_tag="ACG8XG_000664"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772604.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(22719..23267)
                     /locus_tag="ACG8XG_000665"
     CDS             complement(22719..23267)
                     /locus_tag="ACG8XG_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0772605.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(23356..24537)
                     /locus_tag="ACG8XG_000666"
     CDS             complement(23356..24537)
                     /locus_tag="ACG8XG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0772606.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(24773..26281)
                     /locus_tag="ACG8XG_000667"
     CDS             complement(24773..26281)
                     /locus_tag="ACG8XG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0772607.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            26440..26631
                     /locus_tag="ACG8XG_000668"
     CDS             26440..26631
                     /locus_tag="ACG8XG_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772608.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            26834..27751
                     /locus_tag="ACG8XG_000669"
     CDS             26834..27751
                     /locus_tag="ACG8XG_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0772609.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            27744..28817
                     /locus_tag="ACG8XG_000670"
     CDS             27744..28817
                     /locus_tag="ACG8XG_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772610.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            28965..29483
                     /locus_tag="ACG8XG_000671"
     CDS             28965..29483
                     /locus_tag="ACG8XG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772611.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            29464..29856
                     /locus_tag="ACG8XG_000672"
     CDS             29464..29856
                     /locus_tag="ACG8XG_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0772612.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            29932..30867
                     /locus_tag="ACG8XG_000673"
     CDS             29932..30867
                     /locus_tag="ACG8XG_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0772613.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            31000..31779
                     /locus_tag="ACG8XG_000674"
     CDS             31000..31779
                     /locus_tag="ACG8XG_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0772614.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            31772..32089
                     /locus_tag="ACG8XG_000675"
     CDS             31772..32089
                     /locus_tag="ACG8XG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0772615.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            32333..33943
                     /locus_tag="ACG8XG_000676"
     CDS             32333..33943
                     /locus_tag="ACG8XG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0772616.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            34100..34948
                     /locus_tag="ACG8XG_000677"
     CDS             34100..34948
                     /locus_tag="ACG8XG_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0772617.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11572 a         6218 c         6752 g        10438 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccaattcttg atacataaat ccggaggttg ttaataagca
      481 ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat
      541 ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt
      601 tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca
      661 attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta
      721 ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat
      781 aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat
      841 acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa ataactggta
      901 acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt
      961 ctttcaacac gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat
     1021 tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct
     1081 tttctttttc aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg
     1141 aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt
     1201 cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt
     1261 cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa
     1321 tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct
     1381 ctttacatat ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa
     1441 gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat
     1501 aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa
     1561 tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac
     1621 cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat
     1681 agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca
     1741 agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag
     1801 ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt
     1861 ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg
     1921 attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag
     1981 ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa
     2041 tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa
     2101 tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg tagaatctct
     2161 actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga gatattcaac
     2221 catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc agacagtcca
     2281 cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg tcaatcgaat
     2341 tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa gaaaaggaca
     2401 aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta tactacaatc
     2461 agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg agaacacttt
     2521 gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc aggacttgtt
     2581 aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc atcatcattt
     2641 aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact caggaggaga
     2701 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
     2761 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
     2821 aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag aaaaaccaga
     2881 tgtagaccca gaagaaaaac cggatgtgac tccagaacca gatacggatt ctggaaacca
     2941 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaactaga
     3001 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
     3061 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
     3121 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
     3181 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctag
     3241 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
     3301 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
     3361 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
     3421 tatccagaaa acgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
     3481 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
     3541 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
     3601 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
     3661 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
     3721 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
     3781 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
     3841 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
     3901 caacgtgttt aaaagagcga taacaaattg agcttctaat accaaaccaa atgtattgac
     3961 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
     4021 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
     4081 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
     4141 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
     4201 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa tcaataacgg actggatcat
     4261 atcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
     4321 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
     4381 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
     4441 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
     4501 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
     4561 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gaaagagcaa
     4621 aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat
     4681 ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag
     4741 atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg
     4801 aataccttct gcttgttttt gcaacgcagc accttgagga gtcagcgtaa catttacgat
     4861 tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag
     4921 taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt
     4981 ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg
     5041 cttgaggagg gaagtgtagt gtctaatgat ttccttagag gcggcatata aaggaaaaca
     5101 tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt
     5161 atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata
     5221 tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc
     5281 gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc
     5341 gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt
     5401 ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac
     5461 aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg
     5521 aatgtacgat cgggattatg aacttctcca gagcgtccgc ctgtgttgat cattttcgta
     5581 gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac
     5641 acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca
     5701 tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaaatatcag agacaaaaac
     5761 taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaactt
     5821 acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag
     5881 ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt
     5941 aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt
     6001 ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat
     6061 ctcgtgccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca
     6121 atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt
     6181 aatacaaaat ctatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac
     6241 agttactaga ttcttttatt ataccagtta aagaaaacgc attcaaaaaa atagcggatt
     6301 tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg
     6361 ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa
     6421 aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct
     6481 tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct
     6541 tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata
     6601 taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt
     6661 ccaaaaacaa acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa
     6721 gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa
     6781 gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca
     6841 gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa
     6901 aacaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt
     6961 accaacataa cgatattcaa cactaaaata actatagctc ggtcaaatcc taaaacagct
     7021 tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac
     7081 atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata
     7141 aattgtctcg tttctttcat ttatccactt ccttaattca ttaataatac tcttatctta
     7201 ttataactta cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa
     7261 attattatgt ttcattcttc tagaatatac ttctacgtta aaaactgtta aaaaactttt
     7321 actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag
     7381 gaagtagatt aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa
     7441 aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct
     7501 ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct
     7561 gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt
     7621 attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca
     7681 tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt
     7741 accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat
     7801 gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt
     7861 ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat
     7921 caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt
     7981 cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga
     8041 agtaaagatc cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat
     8101 ttggattact tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc
     8161 acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat
     8221 aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc
     8281 gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac
     8341 ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat
     8401 cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact
     8461 ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc
     8521 gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa
     8581 gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc
     8641 tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat
     8701 gcagtgatga attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc
     8761 acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg
     8821 aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt gaccctttgc
     8881 caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct
     8941 ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc
     9001 aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa
     9061 cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta
     9121 atagtatatg atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc
     9181 tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt
     9241 cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt
     9301 ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt
     9361 tgtgcccatt ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga
     9421 aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat
     9481 tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt
     9541 taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg
     9601 aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat
     9661 cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc
     9721 ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag
     9781 cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg
     9841 cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa
     9901 cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag
     9961 gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg
    10021 caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga
    10081 ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc
    10141 caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca
    10201 ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata
    10261 tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg
    10321 gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat
    10381 ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa
    10441 tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag
    10501 ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag
    10561 tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag
    10621 aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa
    10681 aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga
    10741 gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag
    10801 caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa
    10861 aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa
    10921 aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt
    10981 tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt
    11041 tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg
    11101 tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa
    11161 tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac
    11221 acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt
    11281 cactgcattt actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt
    11341 aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc
    11401 agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg
    11461 tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat
    11521 cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat
    11581 attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc
    11641 ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat
    11701 tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt
    11761 acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca
    11821 gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac
    11881 acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc
    11941 cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact
    12001 tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt
    12061 catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga
    12121 ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt
    12181 acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca
    12241 agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg
    12301 actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat
    12361 acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc
    12421 tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg
    12481 attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta
    12541 gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg
    12601 aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct
    12661 aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa
    12721 ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta
    12781 gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt
    12841 atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca
    12901 ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct
    12961 ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc
    13021 gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt
    13081 ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc
    13141 aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa
    13201 caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa
    13261 caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa
    13321 tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat
    13381 aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta
    13441 cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca
    13501 acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc
    13561 attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt
    13621 catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca
    13681 gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg
    13741 ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc
    13801 gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta
    13861 gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat
    13921 taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat
    13981 caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg
    14041 aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc
    14101 ccaatgaaag agattacaag taacgcttct ttggcactag gagaagccta tatggatcat
    14161 cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac
    14221 agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa
    14281 caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt
    14341 tggctagatc caactttgac ttattcttgt gcttatttca atacacctga agatacgttg
    14401 gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc
    14461 gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag
    14521 tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa
    14581 agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa
    14641 ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct
    14701 gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct
    14761 ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat
    14821 attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac
    14881 gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat
    14941 tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc
    15001 tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc
    15061 gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca
    15121 taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct
    15181 ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc
    15241 tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt
    15301 cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa
    15361 aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac
    15421 caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc
    15481 ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc
    15541 aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa
    15601 atacccagat actgcaatta ctagtttgga attagatagt gattggggat cttacttcta
    15661 caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga
    15721 aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa
    15781 aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc
    15841 cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac
    15901 tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag
    15961 ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag
    16021 agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt
    16081 tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca
    16141 tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga
    16201 gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat
    16261 tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat
    16321 tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt
    16381 ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa
    16441 agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca
    16501 caactagctt acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt
    16561 tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg
    16621 gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat
    16681 ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc
    16741 ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca
    16801 cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca
    16861 tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa
    16921 gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga
    16981 tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa
    17041 ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa
    17101 gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata
    17161 atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag
    17221 aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg
    17281 tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag
    17341 ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc
    17401 tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg
    17461 tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg
    17521 gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg
    17581 aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc
    17641 ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat
    17701 tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat
    17761 ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat
    17821 tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc
    17881 aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact
    17941 cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag
    18001 aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt
    18061 attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc
    18121 agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc
    18181 agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg
    18241 tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg
    18301 cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca
    18361 gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga
    18421 agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta
    18481 ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag
    18541 aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa
    18601 atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct
    18661 aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga
    18721 taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact
    18781 gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc
    18841 aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg
    18901 acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac
    18961 aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat
    19021 gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa
    19081 agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt
    19141 agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac
    19201 agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat
    19261 ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa
    19321 agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga
    19381 tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa
    19441 agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ctctaattga tttttccctc
    19501 atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc
    19561 gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat
    19621 tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca
    19681 ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct
    19741 tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca
    19801 ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt
    19861 ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt
    19921 aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa
    19981 atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt
    20041 ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta
    20101 gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca
    20161 ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa
    20221 aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa
    20281 tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca
    20341 cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa
    20401 gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca
    20461 aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag
    20521 taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag
    20581 aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg
    20641 ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa
    20701 aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat
    20761 tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt
    20821 tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc
    20881 atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg
    20941 agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa
    21001 agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg
    21061 tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc
    21121 tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg
    21181 gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag
    21241 tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga
    21301 tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag
    21361 tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag
    21421 aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt
    21481 tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt
    21541 cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg
    21601 caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt
    21661 gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact
    21721 cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg
    21781 actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct
    21841 agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat
    21901 ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc
    21961 ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt
    22021 tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga
    22081 ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg
    22141 gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga
    22201 aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag
    22261 gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata
    22321 tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt
    22381 ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag
    22441 catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca
    22501 ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa
    22561 aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt
    22621 acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac
    22681 gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt
    22741 attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata
    22801 gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc
    22861 tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta
    22921 ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct
    22981 gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa
    23041 ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga
    23101 ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt
    23161 gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa
    23221 gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt catttctcct
    23281 ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact
    23341 ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa
    23401 agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt
    23461 taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag
    23521 gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc
    23581 tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac
    23641 tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat
    23701 ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc
    23761 aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta
    23821 ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc
    23881 tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt
    23941 caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat
    24001 cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag
    24061 gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc
    24121 tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac
    24181 agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat
    24241 aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc
    24301 ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc
    24361 caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag
    24421 ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat
    24481 caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat aactcaaata
    24541 atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt
    24601 tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa
    24661 gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa
    24721 actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt
    24781 aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca
    24841 atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt
    24901 cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg attgataaag
    24961 cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca
    25021 atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg
    25081 ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac
    25141 gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg
    25201 taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt
    25261 cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt
    25321 cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt
    25381 tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca
    25441 tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata atacgtttga
    25501 ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa
    25561 agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa
    25621 tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac
    25681 cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc
    25741 cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat
    25801 tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt
    25861 ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg
    25921 caggctgtcc actatagcga gtgataacga cactgttgat taataagcca taaccacgta
    25981 attcatcgat taaccgcaag atatccatat cataagtaat cccataatca ccacggatct
    26041 tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt
    26101 tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct
    26161 tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt
    26221 ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca
    26281 ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg
    26341 aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt
    26401 attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag
    26461 aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa
    26521 gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt
    26581 gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga
    26641 taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa
    26701 ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac
    26761 aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga
    26821 ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga
    26881 ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga
    26941 aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt
    27001 ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt
    27061 aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga
    27121 tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag
    27181 ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct
    27241 gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga
    27301 gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac
    27361 agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa
    27421 cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa
    27481 gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga
    27541 aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc
    27601 agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt
    27661 cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca
    27721 atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg
    27781 aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc
    27841 ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa
    27901 aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa
    27961 atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa
    28021 cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc
    28081 cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg
    28141 gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg
    28201 gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt
    28261 atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac
    28321 gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg
    28381 tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg
    28441 cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac
    28501 ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc
    28561 agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa
    28621 atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag
    28681 gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg
    28741 gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa
    28801 tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt
    28861 ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa
    28921 atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa
    28981 tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta
    29041 ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa
    29101 aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg
    29161 gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg
    29221 tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg
    29281 cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc
    29341 aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg
    29401 ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa
    29461 aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca
    29521 agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg
    29581 caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc
    29641 ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat
    29701 aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg
    29761 atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat
    29821 ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg
    29881 cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat
    29941 tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct
    30001 atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc
    30061 cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat
    30121 aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca
    30181 atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa
    30241 aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta
    30301 tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg
    30361 aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg
    30421 attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca
    30481 atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt
    30541 ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt
    30601 gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc
    30661 agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca
    30721 aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa
    30781 aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa
    30841 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
    30901 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
    30961 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
    31021 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
    31081 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
    31141 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
    31201 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
    31261 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
    31321 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
    31381 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
    31441 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
    31501 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
    31561 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
    31621 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
    31681 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
    31741 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
    31801 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
    31861 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
    31921 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
    31981 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
    32041 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
    32101 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
    32161 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
    32221 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
    32281 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
    32341 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
    32401 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
    32461 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
    32521 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
    32581 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
    32641 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
    32701 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
    32761 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
    32821 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
    32881 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
    32941 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
    33001 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
    33061 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
    33121 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
    33181 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
    33241 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
    33301 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
    33361 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
    33421 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
    33481 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
    33541 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
    33601 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
    33661 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
    33721 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
    33781 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
    33841 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
    33901 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
    33961 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
    34021 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
    34081 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
    34141 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
    34201 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
    34261 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
    34321 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
    34381 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
    34441 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
    34501 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
    34561 tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
    34621 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
    34681 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
    34741 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
    34801 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
    34861 tgcagaaatc gaaattggtc attttggacc agtcattggt acacatttag gtgaaaaagc
    34921 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaatat
//
LOCUS       DAZKSR010000015        34959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000015 DAZKSR010000000
VERSION     DAZKSR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.015"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG8XG_000678"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG8XG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0772618.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG8XG_000679"
     CDS             complement(2017..2919)
                     /locus_tag="ACG8XG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0772619.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG8XG_000680"
     CDS             complement(2916..3608)
                     /locus_tag="ACG8XG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0772620.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG8XG_000681"
     CDS             complement(3651..4292)
                     /locus_tag="ACG8XG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0772621.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACG8XG_000682"
     CDS             complement(4330..4644)
                     /locus_tag="ACG8XG_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772622.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG8XG_000683"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG8XG_000683"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0772623.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG8XG_000684"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG8XG_000684"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0772624.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACG8XG_000685"
     CDS             complement(5981..6913)
                     /locus_tag="ACG8XG_000685"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0772625.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACG8XG_000686"
     CDS             complement(6910..7686)
                     /locus_tag="ACG8XG_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0772626.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG8XG_000687"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG8XG_000687"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0772627.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACG8XG_000688"
     CDS             complement(10930..12006)
                     /locus_tag="ACG8XG_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0772628.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACG8XG_000689"
     CDS             complement(12011..13291)
                     /locus_tag="ACG8XG_000689"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0772629.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACG8XG_000690"
     CDS             complement(13310..14236)
                     /locus_tag="ACG8XG_000690"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0772630.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACG8XG_000691"
     CDS             complement(14249..15532)
                     /locus_tag="ACG8XG_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0772631.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG8XG_000692"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG8XG_000692"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0772632.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACG8XG_000693"
     CDS             complement(16320..17231)
                     /locus_tag="ACG8XG_000693"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0772633.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG8XG_000694"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG8XG_000694"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0772634.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACG8XG_000695"
     CDS             complement(17697..18395)
                     /locus_tag="ACG8XG_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0772635.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACG8XG_000696"
     CDS             complement(18382..18768)
                     /locus_tag="ACG8XG_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772636.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACG8XG_000697"
     CDS             complement(18915..20585)
                     /locus_tag="ACG8XG_000697"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0772637.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACG8XG_000698"
     CDS             20768..21001
                     /locus_tag="ACG8XG_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0772638.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACG8XG_000699"
     CDS             complement(21100..21531)
                     /locus_tag="ACG8XG_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0772639.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="ACG8XG_000700"
     CDS             21610..22023
                     /locus_tag="ACG8XG_000700"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0772640.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="ACG8XG_000701"
     CDS             complement(22061..23209)
                     /locus_tag="ACG8XG_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0772641.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="ACG8XG_000702"
     CDS             complement(23331..23927)
                     /locus_tag="ACG8XG_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0772642.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG8XG_000703"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG8XG_000703"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0772643.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="ACG8XG_000704"
     CDS             27157..27588
                     /locus_tag="ACG8XG_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772644.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="ACG8XG_000705"
     CDS             complement(27633..28982)
                     /locus_tag="ACG8XG_000705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0772645.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="ACG8XG_000706"
     CDS             complement(29052..30215)
                     /locus_tag="ACG8XG_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0772646.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="ACG8XG_000707"
     CDS             30344..30667
                     /locus_tag="ACG8XG_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772647.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="ACG8XG_000708"
     CDS             complement(30850..32199)
                     /locus_tag="ACG8XG_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0772648.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVPILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="ACG8XG_000709"
     CDS             complement(32268..33221)
                     /locus_tag="ACG8XG_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0772649.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="ACG8XG_000710"
     CDS             complement(33323..34513)
                     /locus_tag="ACG8XG_000710"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0772650.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34959)
                     /locus_tag="ACG8XG_000711"
     CDS             complement(34713..>34959)
                     /locus_tag="ACG8XG_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772651.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9950 a         7640 c         6121 g        11248 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcggtacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc ttccgaata
//
LOCUS       DAZKSR010000016        34770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000016 DAZKSR010000000
VERSION     DAZKSR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.016"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1113)
                     /locus_tag="ACG8XG_000712"
     CDS             complement(226..1113)
                     /locus_tag="ACG8XG_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772652.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="ACG8XG_000713"
     CDS             complement(1110..1979)
                     /locus_tag="ACG8XG_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772653.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="ACG8XG_000714"
     CDS             complement(2116..3402)
                     /locus_tag="ACG8XG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0772654.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="ACG8XG_000715"
     CDS             3675..4253
                     /locus_tag="ACG8XG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0772655.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="ACG8XG_000716"
     CDS             4253..5182
                     /locus_tag="ACG8XG_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772656.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="ACG8XG_000717"
     CDS             5179..6915
                     /locus_tag="ACG8XG_000717"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0772657.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG8XG_000718"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="ACG8XG_000718"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0772658.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="ACG8XG_000719"
     CDS             complement(7811..8320)
                     /locus_tag="ACG8XG_000719"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0772659.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG8XG_000720"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="ACG8XG_000720"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0772660.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="ACG8XG_000721"
     CDS             complement(9621..10712)
                     /locus_tag="ACG8XG_000721"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0772661.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG8XG_000722"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="ACG8XG_000722"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0772662.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG8XG_000723"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="ACG8XG_000723"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0772663.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG8XG_000724"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="ACG8XG_000724"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0772664.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14675..15649)
                     /locus_tag="ACG8XG_000725"
     CDS             complement(14675..15649)
                     /locus_tag="ACG8XG_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0772665.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15630..17513)
                     /locus_tag="ACG8XG_000726"
     CDS             complement(15630..17513)
                     /locus_tag="ACG8XG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0772666.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17531..19756)
                     /locus_tag="ACG8XG_000727"
     CDS             complement(17531..19756)
                     /locus_tag="ACG8XG_000727"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0772667.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19871..20905
                     /locus_tag="ACG8XG_000728"
     CDS             19871..20905
                     /locus_tag="ACG8XG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772668.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20972..21985)
                     /locus_tag="ACG8XG_000729"
     CDS             complement(20972..21985)
                     /locus_tag="ACG8XG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0772669.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22063..22251)
                     /locus_tag="ACG8XG_000730"
     CDS             complement(22063..22251)
                     /locus_tag="ACG8XG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0772670.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22691..22819)
                     /locus_tag="ACG8XG_000731"
     CDS             complement(22691..22819)
                     /locus_tag="ACG8XG_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0772671.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="ACG8XG_000732"
     CDS             complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="ACG8XG_000732"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0772672.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24306..25361)
                     /locus_tag="ACG8XG_000733"
     CDS             complement(24306..25361)
                     /locus_tag="ACG8XG_000733"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0772673.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="ACG8XG_000734"
     CDS             complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="ACG8XG_000734"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0772674.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="ACG8XG_000735"
     CDS             complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="ACG8XG_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0772675.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="ACG8XG_000736"
     CDS             complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="ACG8XG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0772676.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="ACG8XG_000737"
     CDS             complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="ACG8XG_000737"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0772677.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="ACG8XG_000738"
     CDS             complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="ACG8XG_000738"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0772678.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33123..34682
                     /locus_tag="ACG8XG_000739"
     CDS             33123..34682
                     /locus_tag="ACG8XG_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0772679.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9519 a         7494 c         6335 g        11422 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac ctagtataga
    14461 cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc tgatagttaa
    14521 ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat gaacaaaagt
    14581 atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc tttgtcttgt
    14641 aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag ccgatttcat
    14701 ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag ctattgatac
    14761 ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt
    14821 cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa
    14881 atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta tccgtattat
    14941 ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg ccgcattctt
    15001 ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct tcgacaccac
    15061 cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa aattttgctt
    15121 tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct ttttgtccat
    15181 aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa attgtcactt
    15241 gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt
    15301 cattacttga atattgatta tttactggag caatcggcaa atcgatcttt ttattgtcca
    15361 ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac ataccggatt
    15421 taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg
    15481 tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg cctcttgtat
    15541 aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg ccgaagacga
    15601 ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata ataatatctt
    15661 acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat
    15721 tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg
    15781 attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc tccttggtac
    15841 atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga attccccatg
    15901 tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag gtagtcaatg
    15961 atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc ttcgaatgct
    16021 ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt tttgaagtaa
    16081 tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga ccaaaaaaga
    16141 atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc
    16201 tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc cattttttgc
    16261 catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc ttggtcgcat
    16321 aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt gatctttgca
    16381 ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg tccttttttc
    16441 ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt gaagtagatg
    16501 attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata ctccgttact
    16561 cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg aaaatgcccc
    16621 cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt taacggatat
    16681 tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag ctgactgttt
    16741 ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca tttgtcatct
    16801 aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac taagaagaca
    16861 tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc agctgtactt
    16921 cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt taaatcaaat
    16981 tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa ccaaataaac
    17041 attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag accttcttct
    17101 aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga aaagatccct
    17161 tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg tccctcccac
    17221 ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat atgactagga
    17281 acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt ttttggtcgt
    17341 accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg tttaagggag
    17401 gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg tgcatttgat
    17461 tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt catacttttt
    17521 tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat
    17581 cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc ttcgaggtct
    17641 gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg aacgctggtt
    17701 gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta ggagacacaa
    17761 acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa tggccatact
    17821 ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc tttttttctt
    17881 caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata gctgtttctc
    17941 ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct gatacatgag
    18001 ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc atacgctcca
    18061 ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac ccggcatcaa
    18121 accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca gttgtcaaat
    18181 gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttcacct tgtctctgtg
    18241 ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat ttgatgtagt
    18301 caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc cgaacatcta
    18361 accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta cctggctcct
    18421 gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc atttcaggtt
    18481 caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt tcacgtaatc
    18541 catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg tccgcatctc
    18601 tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc tttgcttcat
    18661 caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc cagttattga
    18721 ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca cgtagcaact
    18781 catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg atcatttcag
    18841 gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg ttgatcccac
    18901 cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg ttgccggaat
    18961 aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga tgaactaaag
    19021 caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat acttttgttc
    19081 cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa
    19141 tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc gctttttcta
    19201 gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc actgctctat
    19261 cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa
    19321 tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag ccttcgatct
    19381 ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag tctgatccat
    19441 tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga gaagagtatt
    19501 cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca tttcctgaaa
    19561 atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact ggtttgccaa
    19621 aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa agaaatgtat
    19681 ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta tctgtttttc
    19741 tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact tttttttata
    19801 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    19861 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    19921 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    19981 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    20041 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    20101 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    20161 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    20221 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    20281 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    20341 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    20401 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    20461 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    20521 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    20581 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    20641 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    20701 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    20761 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    20821 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    20881 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    20941 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    21001 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    21061 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    21121 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    21181 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    21241 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    21301 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    21361 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    21421 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    21481 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    21541 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    21601 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    21661 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    21721 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    21781 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    21841 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    21901 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    21961 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    22021 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    22081 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    22141 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    22201 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    22261 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    22321 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    22381 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    22441 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    22501 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    22561 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    22621 atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc ttagtctttc
    22681 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
    22741 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
    22801 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
    22861 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    22921 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    22981 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    23041 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    23101 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    23161 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    23221 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    23281 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    23341 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    23401 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    23461 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    23521 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    23581 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    23641 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    23701 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    23761 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    23821 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    23881 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    23941 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    24001 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    24061 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    24121 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    24181 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    24241 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    24301 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    24361 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    24421 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    24481 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    24541 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    24601 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    24661 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    24721 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    24781 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    24841 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    24901 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    24961 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    25021 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    25081 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    25141 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    25201 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    25261 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    25321 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    25381 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    25441 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    25501 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    25561 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    25621 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    25681 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    25741 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    25801 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    25861 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    25921 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    25981 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    26041 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    26101 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    26161 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    26221 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    26281 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    26341 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    26401 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    26461 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    26521 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    26581 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    26641 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    26701 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    26761 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    26821 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    26881 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    26941 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    27001 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    27061 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    27121 tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc gcgcttaagg
    27181 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    27241 tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag cggataccat
    27301 cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa acagccacac
    27361 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    27421 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    27481 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    27541 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    27601 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    27661 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    27721 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    27781 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    27841 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    27901 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    27961 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    28021 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    28081 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    28141 atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt
    28201 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    28261 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    28321 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    28381 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttccgt
    28441 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    28501 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    28561 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    28621 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    28681 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    28741 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    28801 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    28861 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    28921 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    28981 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    29041 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    29101 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    29161 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    29221 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    29281 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
    29341 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    29401 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    29461 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    29521 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    29581 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    29641 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    29701 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    29761 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag taaaaccaag
    29821 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    29881 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    29941 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    30001 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    30061 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    30121 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    30181 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    30241 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    30301 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    30361 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    30421 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    30481 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    30541 gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt tatcataaac
    30601 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    30661 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    30721 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    30781 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    30841 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    30901 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    30961 ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct tcgatttcca
    31021 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    31081 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    31141 catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct cgagataaag
    31201 gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat cctttggctg
    31261 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atgtcggaaa
    31321 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    31381 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    31441 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    31501 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    31561 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    31621 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    31681 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    31741 aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata tctttgattt
    31801 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    31861 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    31921 aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa cgggtttcgt
    31981 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    32041 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    32101 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    32161 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    32221 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    32281 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatacg
    32341 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    32401 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    32461 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    32521 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    32581 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    32641 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    32701 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    32761 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    32821 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    32881 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    32941 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    33001 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    33061 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    33121 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    33181 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    33241 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    33301 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    33361 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    33421 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    33481 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    33541 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    33601 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    33661 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    33721 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    33781 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    33841 gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt tacaaccgat
    33901 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    33961 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    34021 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    34081 cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    34141 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    34201 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    34261 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    34321 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    34381 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    34441 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    34501 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    34561 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    34621 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    34681 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    34741 attctataaa aaaagaagag gctgggaaaa
//
LOCUS       DAZKSR010000017        34544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000017 DAZKSR010000000
VERSION     DAZKSR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.017"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(156..612)
                     /locus_tag="ACG8XG_000740"
                     /pseudo
     CDS             complement(156..612)
                     /locus_tag="ACG8XG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            777..3139
                     /locus_tag="ACG8XG_000741"
                     /pseudo
     CDS             777..3139
                     /locus_tag="ACG8XG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3507..4429
                     /gene="truB"
                     /locus_tag="ACG8XG_000742"
                     /pseudo
     CDS             3507..4429
                     /gene="truB"
                     /locus_tag="ACG8XG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            4433..5374
                     /gene="ribF"
                     /locus_tag="ACG8XG_000743"
     CDS             4433..5374
                     /gene="ribF"
                     /locus_tag="ACG8XG_000743"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0772680.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5726..6511
                     /locus_tag="ACG8XG_000744"
     CDS             5726..6511
                     /locus_tag="ACG8XG_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0772681.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6524..7375
                     /locus_tag="ACG8XG_000745"
     CDS             6524..7375
                     /locus_tag="ACG8XG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0772682.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7499..7951)
                     /locus_tag="ACG8XG_000746"
     CDS             complement(7499..7951)
                     /locus_tag="ACG8XG_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772683.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8416..8784)
                     /locus_tag="ACG8XG_000747"
     CDS             complement(8416..8784)
                     /locus_tag="ACG8XG_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0772684.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8934..9416
                     /locus_tag="ACG8XG_000748"
     CDS             8934..9416
                     /locus_tag="ACG8XG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0772685.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9453..10463
                     /locus_tag="ACG8XG_000749"
     CDS             9453..10463
                     /locus_tag="ACG8XG_000749"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0772686.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10578..11291)
                     /locus_tag="ACG8XG_000750"
     CDS             complement(10578..11291)
                     /locus_tag="ACG8XG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772687.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11464..12071)
                     /locus_tag="ACG8XG_000751"
                     /pseudo
     CDS             complement(11464..12071)
                     /locus_tag="ACG8XG_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            12555..12782
                     /locus_tag="ACG8XG_000752"
     CDS             12555..12782
                     /locus_tag="ACG8XG_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0772688.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12798..13964
                     /gene="hemW"
                     /locus_tag="ACG8XG_000753"
     CDS             12798..13964
                     /gene="hemW"
                     /locus_tag="ACG8XG_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0772689.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14198..15241
                     /gene="hrcA"
                     /locus_tag="ACG8XG_000754"
     CDS             14198..15241
                     /gene="hrcA"
                     /locus_tag="ACG8XG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0772690.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15335..15898
                     /gene="grpE"
                     /locus_tag="ACG8XG_000755"
     CDS             15335..15898
                     /gene="grpE"
                     /locus_tag="ACG8XG_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0772691.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15949..17778
                     /gene="dnaK"
                     /locus_tag="ACG8XG_000756"
     CDS             15949..17778
                     /gene="dnaK"
                     /locus_tag="ACG8XG_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0772692.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17929..19095
                     /gene="dnaJ"
                     /locus_tag="ACG8XG_000757"
     CDS             17929..19095
                     /gene="dnaJ"
                     /locus_tag="ACG8XG_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0772693.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19177..19806)
                     /locus_tag="ACG8XG_000758"
     CDS             complement(19177..19806)
                     /locus_tag="ACG8XG_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772694.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            19818..19991
                     /locus_tag="ACG8XG_000759"
     CDS             19818..19991
                     /locus_tag="ACG8XG_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772695.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20036..20773)
                     /locus_tag="ACG8XG_000760"
     CDS             complement(20036..20773)
                     /locus_tag="ACG8XG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772696.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20775..22277)
                     /locus_tag="ACG8XG_000761"
     CDS             complement(20775..22277)
                     /locus_tag="ACG8XG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0772697.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22536..24530
                     /gene="uvrB"
                     /locus_tag="ACG8XG_000762"
     CDS             22536..24530
                     /gene="uvrB"
                     /locus_tag="ACG8XG_000762"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0772698.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24540..27356
                     /gene="uvrA"
                     /locus_tag="ACG8XG_000763"
     CDS             24540..27356
                     /gene="uvrA"
                     /locus_tag="ACG8XG_000763"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0772699.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27619..28884
                     /locus_tag="ACG8XG_000764"
     CDS             27619..28884
                     /locus_tag="ACG8XG_000764"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0772700.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28888..29619
                     /locus_tag="ACG8XG_000765"
     CDS             28888..29619
                     /locus_tag="ACG8XG_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0772701.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29754..30638
                     /gene="rapZ"
                     /locus_tag="ACG8XG_000766"
     CDS             29754..30638
                     /gene="rapZ"
                     /locus_tag="ACG8XG_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0772702.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30635..31633
                     /gene="yvcK"
                     /locus_tag="ACG8XG_000767"
     CDS             30635..31633
                     /gene="yvcK"
                     /locus_tag="ACG8XG_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0772703.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31667..32602
                     /gene="whiA"
                     /locus_tag="ACG8XG_000768"
     CDS             31667..32602
                     /gene="whiA"
                     /locus_tag="ACG8XG_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0772704.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33145..33795
                     /locus_tag="ACG8XG_000769"
     CDS             33145..33795
                     /locus_tag="ACG8XG_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772705.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33982..>34544
                     /locus_tag="ACG8XG_000770"
     CDS             33982..>34544
                     /locus_tag="ACG8XG_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0772706.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11458 a         5842 c         7327 g         9917 t
ORIGIN      
        1 caaaaactcc cgcaaatcct gtgaaatcag gggttgcggg atttttttgt tttcattaag
       61 acgtattacc taagagttcc atgactttag tcttgcggcg tgtcagctaa ccaaaaaaga
      121 gaaaaagaaa agtaaaaaat tcgactttct tttttctagt agtatataaa tttttgcata
      181 tagataaaga tcaagatacc gataacggaa gctagattgt aatctatttt agtgacagtc
      241 aggtatagcc aagtaactag aagatcagtg gaacctgcac cataaaagtt tgagtttgtc
      301 ggtccgttga cagttaaaag aaaaatcacg ttgaagttat tgatatttcc gattacgccg
      361 cttcgatttg gtcacgatcc aaattttgga taatccctgt agaaattagc atagtagctg
      421 ggataccaat tcacatattg acaaagataa cagagacttt tacataaaac ggatcaatca
      481 ggaaaggaat tgctcgatcc agccccagtt ttccagcaat gtgttaactg gcccagcgtc
      541 attcagcaaa ttatgcatga tgaggagcga aatgaactgc ggaacagcca ttgtgataac
      601 gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt
      661 atacctgttc atttggctag atataaaatc caaacaaatg aacagaagtg cttattaggt
      721 ttcaattggg agtatctgcg ctagaataag agtaataatg aaaaggagtg ttatgtatgg
      781 attattctag taaagaatac tttgataaaa tgacggcttg gtggcgtgct gctaattatc
      841 tttctgttgg gcaactttat cttaaagata accccttact gagacgtaca ctgaaacccg
      901 aagatgtcaa aaaacatcca atcggtcatt ggggaacgat tcctggccaa aattttattt
      961 atgtccattt gaaccgagtc atcaataaat atgatttgaa catgttttat attgaagggc
     1021 caggacacgg cggacaagtc atggtatcga atgcttattt agatggaagt tacacagaga
     1081 tctatccaga agtaacggaa gatgagacag gaatgcaaaa attattcaaa cgattttcat
     1141 ttcctggagg cattgcttct catgcagcac cagaaacacc aggttctatc catgaaggcg
     1201 gagagcttgg atactctctt tcacatgcag tcggagctgt attagacaat cctgaagtaa
     1261 tctctgctgt cgtgattggt gatggtgaag cagaaaccgg tcctttagct ggaagctggt
     1321 tctcaaatgt ctttatcaat ccagtgatag atggagctgt tttaccgatc cttcatttaa
     1381 atggtgcaaa aatagccaac ccaacgatct tggcacgtaa atcagatgga gaattagcaa
     1441 attactttaa cggacttggc tgggaacctt tctttattga aggaaatgat tcagaaaaat
     1501 taaatcctgt tatggctgaa aaaatggatc aagcaatcga gaaaattaaa tccatccaaa
     1561 aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg
     1621 tacgcacacc aaaaggatgg accggtccaa aagaatggga cggggaacca attgaaggaa
     1681 ccttccgagc acaccaagta ccgattcctg tagatcaaga acatatggat catgcagatg
     1741 ctttactccg ttggctgaaa tcctatgaac cagaaaaatt atttgatgca caaggacgta
     1801 ttttagaaga aatcagagaa atcgcgccta ctggtgacca aagaatggca aaaaatccta
     1861 tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc agattggaaa aaatatactt
     1921 tgcaatttga aaaaccaggc tccgttaaag cagaagatat gaccgaactt ggtaaattcg
     1981 ttcgcgagat cattgagaaa aatccagaaa atttccgtat atttggacca gatgaaacaa
     2041 aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg
     2101 aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt caattatcgg
     2161 aacaccaaga tgaagggttc ttagaagggt acgttctgac tggacggcat ggttttttgc
     2221 aagttacgaa tcattcttgc gagtggttga ctctatgttg actcagcact tcaaatggat
     2281 gcggaaatca catgaccttt cttggcgaaa tgattatcct tcgctaaatc tcattgcaag
     2341 ctcaacagtc ttccagcaag atcacaatgg atatagccac caagatccag gaatattgac
     2401 acatttggca gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg atgcaaatac
     2461 attgctcgca gtcatggata aagcattccg ctcgtcagaa aaaatcaatt tgatcatctc
     2521 ttccaaacat ccgcgtgctc aattttactc agcagaagaa gcagccgtct tagtgaatga
     2581 aggcttgaaa atcattgatt gggcttcgac agcaaaagag gaggaacctg aattggtcat
     2641 tgctgctgca ggaacggaat ctaacttaga agcattagca gctgtaactt tgctgctgga
     2701 agaattccca aaactaaaaa ttcggttcat caatgtcgta gatttattga aattacgtca
     2761 tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt gatcaatatt ttacaaaaga
     2821 caaaccgatc ttatttgctt tccacggtta tgaaacattg gtccgcacga ttttctttga
     2881 tcgtcataac catcatttaa tgattcatgg ctacaaagaa aatggagata tcacgacacc
     2941 atttgacatg cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag acgcagcttt
     3001 aaaaatcaaa ggaagtcaag cagaagattt tgcgaaaaaa atggatcaga aattacaaga
     3061 acatcaaaac tatattcgtg aaaatggtat tgatctgcca gaagtattgg attggaaatg
     3121 gaaaaatctt gaccaataaa atgataaaat cgatcccgca aaatagaatt ttttacattg
     3181 attaagaaac tgaaaaaata aataaaaaaa acggaaaaaa gactggaaga atggccagtc
     3241 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
     3301 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaagcataac
     3361 atgatttcta tttacgtgag catgaatttt gttacgtttt tattgccgaa aaataaaaac
     3421 cgttcattat gatgtcaaat tcgtcagtaa ttgatgcaat gtgttataat aattcagtta
     3481 aatcaaaaat gaaatggaga aaagacatgg acgggttatt acctttatgg aaagaacgcg
     3541 gaatgacaag tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg
     3601 gacatggggg tacgcttgac ccagacgtag acggtgtgtt gcccatttgt attggaaaag
     3661 ccacaaaagt tatcgaatat ttgacagatt ctgggaaaat ctatgtaggc gagataactc
     3721 taggtttcag tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag
     3781 atatgtcttt atctgaaaaa aaatagatga agtgatggct tcatttattg gtgagatcac
     3841 ccagatacca ccaatgtact cagcggtaaa agtcaacggt aagcggttat atgaatatgc
     3901 ccgcaataat gaaacagtgg agcgtcctgt tagaaaggct cagatttacc gttttatccg
     3961 gacaagtgaa ttgacaagag acgaagtgac aggaaccatt tcttggcgct tcgaagtaga
     4021 atgcgggaaa ggaacctatg tccgcacatt agctgtagat acaggcaaga aacttggtta
     4081 ccctgcgcac atgtctgatc tgacccgaac agcaagtgcc ggtatgtccc agaaggatgc
     4141 tctgacatta gaagaagtag ctcaagctgt gaaaaatggg acaatcgatg attgtttgct
     4201 gccaatagaa aaaggagtgg aacggttcaa acgagtagat attactgatg aagtatggca
     4261 gaaagtaaaa aacggtatga gacttgatta tactgtattt gcgctcccag aaatgccgtc
     4321 agaagaaatt gctttgtttt ataaaggaaa agttgtcagt atttatcagc caaacccgaa
     4381 agaaaaaaac aaattaaaac ctagtaaagt attgagaaat gaggaataac gaatgaatat
     4441 catcaagatc agacatcctt accaatcaga acaaattcca gaagacgatg tcgttttagt
     4501 gctgggattt ttcgacggcg tgcatatcgg acaccagaaa gtcataaaga caggtatgga
     4561 aattgcacaa aaagaaggat tgaaattagc attgatgact ttcaatcagc atccatctat
     4621 tgtctttaaa aagatcgatc ctagcagcgt gaaatatttg actagcctta agcaaaaaga
     4681 agaaaaaatg gccgctttgg gtatagatta tttatatgaa gttgatttta cttcttcttt
     4741 tgctcattta gcaccgcagg aatttgttga tcaatacatc gtagggttac atgcaaaata
     4801 tgctgtttcc ggttttgatt atacgtatgg accaaaagac attgcagatg tcgctcattt
     4861 tcctgaatat gccaaaggaa gattcaaaat cgtcacagtt tctaaagaag aagaacaagg
     4921 tgaaaaaatc agctctaccc gtattcgtgg attactagac agcggacaag taaaagaagc
     4981 agcaaaacta ttaggttgca catatgaggt agaaggggta gttgtccatg gcgatgcacg
     5041 aggcagacta ctaggattcc ctacagcaaa tgtcaaagta aagagcacgg ttcatttgcc
     5101 taaagaaggt gtttatgtgt cggaaataaa aattggggaa acatggtatc cagccatggg
     5161 ttcaattgga cataatgata cattcggtga aggacgggag ctgactgtag agttgtatat
     5221 tttggacttt aatcaagata tctatggtga acatgtcgcg gtacgctgga atcaattttt
     5281 aagagatcaa gtgaaatttt ctagtgtaga ggaattgatt gaacaactca aggaggatga
     5341 acaagcaaca gctgaatatt tttctcagca ataaaacatt atgcataaaa tctcttgttt
     5401 atgtataact agcaattaca ataaaatggg tgataaataa gcccaggaaa ggggctgtga
     5461 caaaagtctt gtcacagcct ttttttccaa ataaatggtt tttcagaagt agcttcttcg
     5521 gaaataagcc gaaatttctt gaagctgacc acttctgtca caacctcttt ctttatagaa
     5581 ataaaaggac actagcaatt catgaaaacg attgctagag cgtatcattt gctttagact
     5641 atatatcttg gtgtacactc actttaaggc ggaaataaaa gttatccgta ataacaggtt
     5701 taggtatgaa aaaggagtgg gtactatgta caaggaatta gaaaacaaag tcgcagtagt
     5761 aactggtggc tcaaagggga ttggaacagc gattgctcaa cgattcggga aagaaaaaat
     5821 gaatgtagtt gtcaactata actcggataa agaaggagca gaaaaagcag tcaaagcaat
     5881 cgaagaggca ggcggaaaag cagtctctgt acaagcagat gtcagttctg aagaaggaat
     5941 acagcgttta cttgatacgg ctttagaaaa ttttgggaca ctggacgttt gggttaataa
     6001 tgctgggatg gaaaatcagc atccaaccca tgaacttagc cttgaagatt gggaaagagt
     6061 gttaaaagtc aatttgactg gcgtttttat cggaacgaaa gcggctttaa attactttac
     6121 agagaataat aaaaaaggaa atatcatcaa tatgtcttct gttcatgaac agatcccatg
     6181 gccaacgttt gctcattacg ctgcttcaaa aggcggggta aaattattta ccgaaacggt
     6241 agcaatggag tatgcaccga agggaatcag aatcaatagt ataggccctg gtgctatccg
     6301 tacacctatt aatgcggcaa aatttgatga tcctgaacaa aaagaactct tggaaagtat
     6361 gatcccacta ggacgcgtag gtgaaccaaa agaaatagcg gcgacagctg catggttggc
     6421 ttcagacgaa tccagttatg ttacaggtat tacactattt gcagatggtg ggatgacttt
     6481 atatccttct ttccaaggtg gaaaaggata agggtgaata aaaatgggtt tattgattgc
     6541 attgatcccg gcagttggct gggggatcca gccactgatt ttgaaaaaga ttggtggcag
     6601 accaacaaat gagatattag gaactggtat tggagcggtg cttgtcggct tactcgttca
     6661 attattcgct tctccagctg gtatttcagt gactacattt ttgattagtt tactttctgg
     6721 tgcattttgg gttattggac aaacaggaca gtatacatct ttcaacttga ttggtgtttc
     6781 taaaacgatg ccaatttcga ctgcattaca gttagtcgga acctcgttga ttggtgtctt
     6841 cgcttttgga gaatggtctg gaagtatggg gaaaatcatt ggagcagcag caatcattct
     6901 tcttgtattt ggttctgctt taacggcaat cagtgatgat cctggaaaaa aaggcggtat
     6961 cacaaaagga attagtctat tagcatcaac aagtattggt tattgggtat acagtgcatt
     7021 gcctaaacta gttgatgcaa gcggactttc cattttcttt ccgcaaatga tcggtgtctt
     7081 tttaggggca gtgatttatg tgattttccg taaaccaacc gcattttctg aagacaagag
     7141 ttggaaagca acgatcgttg gagccatctt tagtgtgtca gcattggctt atattttttc
     7201 agcaaatgaa aatggggttg ctactgcata tatcattaca cagctaaacg tagttatctc
     7261 tacattaggc ggtatgtaca tcctgcatga aaataagaca ccaaaagaaa aaaaattaac
     7321 gattatcgga ttattgctga tcgtagtagg aagcatggtc acggtcttta tttgaggata
     7381 aagatggtaa aatttcattt attaaaaaaa tatttgtttg aaccaaacag taagtactta
     7441 taaaaaaata gcgctagaat ttccaacatc tctggaattt ctagcgcttt ttaattgttt
     7501 aagatacaag ggaaatatct gtctggatca ataatttttc attgatccat tgactaaagt
     7561 ttacgacata attgaagtta ccaccgatta gtatctccgc atgattgtct ttataaagat
     7621 cgtattgtaa aagacggtct tttttatttg cgtaaacatc gttatggtac tggactaatt
     7681 gttctcggaa ttgattgatt gctgtaaaat cacgatattg aaggttgaaa cgcatcgtga
     7741 ttggtgcatt tagcatgaat cgctgcattt cttttgctgc ttcctctgct tgatctccgt
     7801 ataggaactc aaaaagatta cgttcagaaa atttccattg cccgccaacc tttgttcctt
     7861 ttagttttcc ttcatgaaga taatttctga tagaacgtgt agtaacacct aattgttcgg
     7921 ctaattcatt tactgagtaa aaatctttca atgttcaccc tcctatgtga atggttctac
     7981 tgacttagtt gatatttgct taatcacgaa atgtagaaag aacgatttta attaaggtca
     8041 ttgtaaaaat caagtcagaa aattctaact tttatttctc taaagaaatt cgttctctcc
     8101 taaagtgata ttatatttaa atatacctga aaataatggg cagtattatg aaagatcact
     8161 gatattttct cgtatttttt ccttattcaa aagttttgca ggaaaattgc tgaaaaaata
     8221 attttactaa gtagaaaatt caaagccatt caatcagtga tttttttcag cacaagaaag
     8281 actccttata aaaatagcag atactttgtc aattaagtgt ttaaccaggg atgataaaaa
     8341 ggataggatt gaacaaaacc tgtataaggt gatgaatagt ttcctcgcca gtaaaaggtt
     8401 tattcgtata aagctttatc gccaaaattt atcatgacct tttttatctt caatattaaa
     8461 tgaaatcaat tgttcaatca attcccagtt gattacactt ttccaaggaa ctttgatgac
     8521 gttatccgta tatacgtagc ctgcttcttg gatatttttt tcaagggcac gaatcgctgc
     8581 tttttccggt gcgatagaaa aatgattttt agcaaaacta aatgcaataa taaaggtacc
     8641 atggtcagta aacataggtt ggttccattt tacagttgtg tctagctggg gatacgtttc
     8701 tgttatctta gcaaaaaggt ctttgatctt ttgttgatgt tctttttctt tgatttgatt
     8761 gatgatcggc tgaaattttt ccattctaaa acgcctccat taagtggttt gatttatttt
     8821 accagaaaaa caatagttat gtataaaaaa cgaacaaaac acaagtaatg ttgtaaccgc
     8881 tatatttata tgataagatg tctcaaataa taaaatagaa aagagacata aaaatgattg
     8941 acttggcaaa gaaattctta cacgaaaata tatattttga gttgtatcat catcgagcca
     9001 tttatacaaa tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga
     9061 ccaaaagctt attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta
     9121 cgaaacaaac ggatttcaaa gcattaggga agatcgtcgg taaaaggctt tctattgttt
     9181 catcagaacg catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt
     9241 atgaaaaaca gattccgatt attattgatg aagagctctt atcaaatgaa aaacttgttt
     9301 ttgcaccagg tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca
     9361 tccagctact tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa
     9421 gtaaaatagt gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa
     9481 atgcaacgat tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac
     9541 tcgaatcagc tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc
     9601 tttggacgca agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc
     9661 aaatgatgca agatgaaaat atccaaatga tctttccgac ttggggagga gaattattga
     9721 ttgagattct cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt
     9781 cagatattag tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg
     9841 gaactaacct tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa
     9901 gtgtactgaa aacaagagaa ggagaatcga ttctacagca atcttctgaa aagtatcaaa
     9961 aagtgtggcc ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt
    10021 ggcaatcagt cagcggagat gcagtaagga tgaagggacg attattaggt ggttgtattg
    10081 atacgatcag gcatttaatc ggtactcctt atggagatgt ccaatctttt aaaagaaagt
    10141 ataatgatgg aaagccgatc ttgtggtttc tagaaaactg tgagctatcg tcagttgatt
    10201 taagaagatc acttgttcag atgaaactgg caggctggtt tgatggcagc tcggggatca
    10261 tgttcggtag aagtgcggca gaggatttgt tagattataa aatgaaggat gtatacgaag
    10321 atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc
    10381 cacaactgac attgattaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga
    10441 ctgttttgca aacgtttatt taaaaaattt tttcctgcct tggagtctat caaagaccaa
    10501 taaatctaaa tagaaaagaa agtgaaagaa gctgacaaaa tgctgtcagc ttctttttgt
    10561 ataacatatt agaaagtcta attatctata actgtcacat cgattaactg tttcacgtca
    10621 ttgggatttc ctgtaggaat agctactaca gatgcattat caatgatttc ttgagttgtt
    10681 tctctaatga acgctggata agatgtttga tctgcaggca tttgccaatg tgaaggaact
    10741 ggataactag tgattgtccc gtcatggtta cttgaataca ctaaaactcc ctggtaactg
    10801 tatttcccag tgagcataac cacatctttc ggatagactg cactccctgc atcataaggg
    10861 gcgataggtg ttcctgcagg ataatgaata acatgtaatt cgaaatcacc cgatgtatct
    10921 ctaaaagtat tacctctaat agctagccaa acacgtgcgt actcaatttg tagatcagaa
    10981 tatcctgcta acggatctat tgactgagaa gactgtgagg aggttgtgtc tattgttgaa
    11041 gaagaaatat ttgtctcaac tgtactacta gatgtagaag aagtattggg ctctgagact
    11101 ttactgcttg ttgaagatgt attcgtttca gtagaagctg atttctgagt acttgatttt
    11161 tcagctgaac tggtttgtct gattgaactt ttgtctgtgg tcgcagatgg atcactattt
    11221 tctttactag gagacgtaca tcctacaagg aataaagaga ccaacaataa aataaaacct
    11281 aactttttca tataaaatac ctcattattt ttttaatacc agtatagcat aatcctgaat
    11341 ttttgaatcc gtaaaacctt gtctatgaat ataaatagag tgtatcttct ttgataaatg
    11401 tcagaaaaag tgtgacaaat aatcatcagt aaaattactt gtcacactat tgataaggag
    11461 tatttaattg aagagaatga ttaggagaaa ggatctccgt actttttata gtaatcatag
    11521 aatccaagtt tgtatagacg aatcgttttt tctcttattt cttctgtttc aaaatggact
    11581 gcttgaagaa tcaagtctgt gaattctata ggagctgttc cattcgcagt aatcaaatta
    11641 cgatctttca cagcttgttg ttctataaaa tcttctttat tctgataatt ttccgattct
    11701 aaccaaggaa attgcgcatt tccggtgtgt ttgtaacctt gcaataatcc gctctgagct
    11761 aaataatcaa ctgccccaca aatagcagct actggtacgt ctgtttttaa caatttatcg
    11821 attatttgat ataaatgatg attttttatg tcccatgaat tgccaccgat cataataaaa
    11881 aggtcaactt tttcaggtat ttgttcgatt aagtaatcga tttttgtttt taatcctcca
    11941 atggaagtga cctctgattg gatagaagca gttttgtttt gccatttctc attttggttt
    12001 agtgtgctag taatataagc accttcccaa tcggcatatt cctctaatag aaacgtaatc
    12061 gcagttttca ttttcaagct ctcctttact atctataaca aggacaacag ttttaaccta
    12121 cagacattca ttaaaattgg gtagcgacag cgaaaacaac aattcttaag ttttagacag
    12181 agtatacaga tagtcaactt aattaaatac gaactaaata aaaatgacag gtaatcattt
    12241 ttgtttacca gcattataac attttgcata catggatgac aaaagaacca attcccactt
    12301 gcttgagtac aatattaaaa gaaactggaa ggcaaaaagt aattatagaa acgaaaaata
    12361 aatcatatcg ggcactcaga catgaaaaca accctagaga tatataacta ggtaaccagt
    12421 ttaatgaaag aaaaaatcat ccaagaagtc gattcttgga ttttttagtg gatttgttcg
    12481 ccaaatacgt ttagtatgcg tatatgagag gttgtaagca aagcgttgtg gtctgagcac
    12541 ttaaggagga atctgtgaaa atagcttttc atatttttgc agattttgac ttaatgccga
    12601 aggtcagctt tgcgattacc gtttatcgag aggctgtaaa agcaccgtgt agctccgatg
    12661 acaataagca agcattttta gagctaggtt tccttgattc taaatatgcg gcttatttca
    12721 atgagttggt gcttgaacgc gattttaaga aggtcgaaaa caggcacgac ttttcgacgt
    12781 aaggagaaag aatttcaatg atagaaacaa taaatcccgc agaaaacaag gtttctgcgt
    12841 acattcatat cccattttgc gagcatatct gctattactg tgattttaat aaagtttttt
    12901 tagaagggca gccagtagat gagtatgtgg aaatgctgct gaatgaaatg aaaataatgc
    12961 tagaaagtca tccgacagcg aatttagaaa cactttacgt agggggcggg acaccaactt
    13021 ctttatctgc taaacaactt gatcgactgt tatcaggtgc tagagagatc ctgccttttc
    13081 aaaatggtaa tgagtttact gtagaagcga atccaggtga tttgacccgt gaaaaacttc
    13141 aagtaatgaa aaactatggc gtcaatcgtt tatccatggg agtgcagaca tttgataatc
    13201 gcttattgaa aaaaattgga cgtaaacaca ccgcagaaga tgtgtatcaa actatgcaat
    13261 ttttagaagc agaaaatttt accaatgtaa gcatcgattt gatttatgca ttaccgggac
    13321 aaacactaga aggatatctg gatactttgg aaaaagcttt ggctttggat ctgcctcact
    13381 attctttgta ctcattgatc ctcgaaaaca aaacaatgtt catgaactgg gtacgacaag
    13441 gaagactcga actgcctgat caggaaacgg aaaccagaat gtttgaagag acgattgaag
    13501 caatggaaaa gcatggacgt caccaatatg aaataagtaa ctttgggctc tccggacatg
    13561 aatctcaaca taacctcatg tactggaaca atgcccacta ttttgggttt ggagctggtg
    13621 ctagcggtta tcttggagat attcgttacc gcaacaaagg accaatccaa cactatttac
    13681 gcccattacg agcaaatgaa cgcccggtat tggaagaaga aaaattgaca cgaaaaaatc
    13741 aaatagaaga agaaatgttc ttaggactaa gaaaaaaaac agggattact aaaaaacatt
    13801 tctatgatcg ctatcaaaaa acaattgaat cggtgtacgg tgatgtgcta agggaattag
    13861 agcaagaagg gttacttgtt aatgaagaag accgtgttta tttgacacca agagggacct
    13921 ttttggggaa caatgtgttt gagcgctttt tgtttgaaaa atagtttaaa gaatatttgt
    13981 tttttatctg aatttaaata aaaaaagttc aaaaatacaa taaaggacta ttgaaacgtt
    14041 gatataactg gatttacatc aattatttag aattagcact cttgtgttta gagtgctaaa
    14101 aaatggagtc tttctcttga caggagaagg gcttcttgct atatttataa gtggattagc
    14161 acttagaata tatgagtgct aatgagaggt gaggaagatg ttaacaaagc gacaaaacga
    14221 cattttacgt ctgatcatcc aacactatac gagtagtggc gtgcccgttg gttccaaaac
    14281 attgatgtct gagggagtca aggccagctc agcaacgatt cgcaatgata tgaaagcatt
    14341 agaagatgaa ggattgctgt tgaagactca ctcttcttct gggcggattc catctgcttt
    14401 aggctatcgt tattacgtgg atcatttgtt aaaacctgct cgcgtagcag aagatgattt
    14461 gctgctgatt cgtcagtctc taggcagaga atttcatgaa atcaatgaga ttattaagca
    14521 atctgctgag atattatctg agctgacaag ctatacgact ttttcattgg gacctgaaat
    14581 aaaagaccgc cgactgactg gctttcgtat cgtacccttg aataatcgac agattattgc
    14641 tatcgtcgta acagatatgg ggaatgtaga aagccaagtg ttcactcttc ccgaatatat
    14701 gggaagtcag gaactagaaa aaatggtaca gattgtaaac gaccgtctag tcggagatcc
    14761 tctgatgacg gtgtaccata aacttcgtac ggagattccg atgattcttc ataaatattt
    14821 ccaaacgaca gaagggatgt ctcagttatt tgacaccgtt cttggtcatg tttttgaaga
    14881 taaggtttat gtcagtggac aaatgaatct cttggattat gagccccgtc aagatcttga
    14941 tcagttcaaa tcgatgtttt cttttatgaa agactcagac ggacttacac aaatgatcac
    15001 tccgatggat agcaatattc atatcaagat tggtgctgaa ttaggcaatg atttattgca
    15061 gaatatgagt atgattcaag caagttacga gatagatggt catggaagag gtacaatagc
    15121 cttgttaggg cctactagta tgccttattc tcggatgttc ggtctagtcg atgtttatcg
    15181 aagagaacta gcagctaaac ttgctgatta ctaccgttcg cttgacttgt caaactcgta
    15241 atgtgaataa cgaagctatt tgcagctgat cgttctctct ttcttagtaa gagcaatcaa
    15301 acagctgact gaataatgaa aggaagactg tatcgtgaaa gaaaatcaag aagaactaga
    15361 taaagaaatg acagatgctc agccagaacc ggaaatcgat gtcgaagctg cagaagattc
    15421 gggtatttca gaagctgaag cggaagaatt cgaaacagcg aaattaaaag ctgagttaga
    15481 agaaatggaa gataaatact tgcgggctcg tgctgaaatc gcaaatatgg caaatcgagg
    15541 aaaaaatgag cgtgagcaat tacaaaaata ccgttcacaa gatttagcca aaaaattact
    15601 tccatcaatt gacaacctgg aacgtgcatt agccacagaa gtgtcagatg atcaaggggc
    15661 aggtttgaaa aaaggcgtgg aaatggtctt ggaaagtttg cgtaacgcac tggaagaaga
    15721 aggaattgaa aaaatccctg ctaaaggaga agcctttgat cctaatcttc atcaagcagt
    15781 acaaactgtt ccagcgactg aagatacacc agccgatacg attgtagaag tactacaaga
    15841 aggctacaaa ttacatgatc gcgtgttaag accaaccatg gtcattgttg cacaataatt
    15901 gtgcggatat aaaacttaca ctttcaatta aagaaggaga cgttaattat gagtaaaatt
    15961 atcggtattg acttaggtac gactaactct gcagtagcag tattggaagg aaacgaagca
    16021 aaaatcatcg ctaacccaga aggaaaccgt acaacacctt ctgttgtatc attcaaaaat
    16081 ggagaaatcc aagtagggga agttgcaaaa cgtcaagctg tgacaaaccc aaatacgatt
    16141 tcatcaatca aacgccatat cggtgaagca ggttacaaag tagatgtaga aggtaaatca
    16201 tatacaccac aagaaatctc agcaatgatc cttcaatata tcaaaggatt cgcagaagac
    16261 tatttaggtg aaaaagtcga aaaagcagtt atcactgtgc cagcttactt taatgatgca
    16321 caacgtcaag caacaaaaga tgctggtaag atcgctggtc tagaagtaga acgtatcgtc
    16381 aatgaaccaa ctgcagctgc tcttgcctat ggtttagata aaacagatcg cgacgaaaaa
    16441 atcttagtat tcgaccttgg tggcggtact tttgacgtat ctatccttga actaggagac
    16501 ggtgtctttg atgtattgtc aacagctggc gacaaccatc taggtgggga cgactttgat
    16561 aacaaaatca tcgactacat ggtagcagaa ttcaaaaaag aaaacagcat tgacttgtct
    16621 caagacaaaa tggctttaca acgcttgaaa gacgctgctg aaaaagcgaa aaaagattta
    16681 tctggcgtat caagcacaca aatcagcttg ccatttatca cagcaggaga atctggacca
    16741 ttgcatttgg aaatgacatt gacacgtgca aaatttgatg aattaacagc tgacttagta
    16801 gaacgtacaa aagtaccagt acgtcaagcg ctaaaagatg caggtttatc acaatcagaa
    16861 atcgacgaag ttattttagt cggtggatct actcgtatcc ctgcagttgt agaagcagta
    16921 cgtaaagaaa caggaaaaga accaaacaaa tcagttaacc ctgatgaagt agttgctatg
    16981 ggtgctgcta tccaaggtgg cgtgatcact ggtgatgtga aagatgttgt tttacttgac
    17041 gttacaccat tgtcattagg tatcgaaaca atgggtggag tgttcactaa attgattgac
    17101 cgcaatacaa cgattccaac aagtaaatca caagtattct caactgcggc agataatcaa
    17161 ccagcagtag acatccatgt attacaaggt gaacgcccaa tggctgctga caacaaaact
    17221 ctaggaagat tccaattgac agatattcca gcagctcctc gtggtattcc acaaatcgaa
    17281 gtaacattcg atattgataa aaacgggatt gtaaatgtat ctgcgaaaga tctaggtaca
    17341 caaaaagaac aaaaaatcac gatcaaatct tcttcaggtc taacagacga agaaatcgaa
    17401 cgtatggtaa aagatgcaga agcaaatgca gaagcagata aagctcgtaa agaggaagtt
    17461 gacctacgta atgatgtaga tgcattgcta ttctctgtgg acaaaacatt gaaagaatta
    17521 gaaggcaaag tggacgaaga agaagtgaaa aaagcagaaa cagctcgcga tgaattgaaa
    17581 gccgctgttg aagcaaacaa catcgaagaa atgaaaacta aacgcgatgc attgaacgaa
    17641 atcgttcaaa acttgactgt taaactatac gaacaagcgg cacaacaaca agctcaagaa
    17701 aacccagaag cagctcaagg cggagcagat gatgtcgttg atgctgattt tgaagaagtc
    17761 gatggcgacg ataaataatc agttcactga atttcgagag aaggccaaag ttgctacttt
    17821 ggctttttct tttgaaaaat agtttgaagt atcggttttt gtttcaaaca aaatatgata
    17881 tgataacaag cgacgtgaaa atgatgagaa ccaataggag gaaagcctat ggcaacaaaa
    17941 cgtgattatt atgaagtctt agggctctca aaaggagcat cagaagatga aataaagaaa
    18001 gcttatcgta agctttcgaa aaaataccat cctgacatca ataaagaacc tgatgcggaa
    18061 gaaaaattca aagaagtatc agaagcatat gagattttaa gtgacccgca aaaacgcgct
    18121 gcatatgacc aatacgggca tgcaggcaca gatccaaatt acggtgctgg tggagctggt
    18181 ggcggatttg gtggattcgg cggcttctct ggtggcggct tcggtggttt tgaagatatc
    18241 ttcgactctt tctttggagg aggcggtcgg tcagtcgatc caaatgcgcc tcgtcaaggt
    18301 gctgatttgc aatatacggt cgacttgtct tttgaagaag cgatttttgg tgtagaaaaa
    18361 gaaatcaagt ataatcggga agaaatctgt catacttgtg gtgggaacgg tgcaaaacca
    18421 ggcacacaac caactacttg tcataaatgt catggtgcag gtacgattaa tgtagaacgc
    18481 caaacaccac ttggacgagt gatgagccgt caaacttgtg atgtttgtca tgggacaggg
    18541 aaagaaatca aagaaccttg tccaacctgt catggctcag gacatgagaa gaaagcacat
    18601 tcagttaaag tgaatgtacc agctggtgta gaagaaggac aacaaatgcg tttagctggt
    18661 caaggagaag ctggagaaaa cggcggacca tttggtgatt tgtatgtggt cttccgtgta
    18721 gaagaaagtg atatctttga tcgtgaaggt tctgaaattt actatgaatt gcctttaagt
    18781 tttgtacaag cagctttggg ggatgaagtc caagtaccaa ctgtccatgg agatgtgaaa
    18841 ttgaagatcc ctgctggaac acagacaggt acgaacttcc gtttacgtgg aaaaggcgct
    18901 ccgaagttac gtggtggtgc aacaggcgac caacatgtca aagtaaaact gatcacaccg
    18961 aaaaatctaa atgaacaaca aagagaagca ttacgtgcat tcgctgaagc tggcggcatc
    19021 aaagttgaag aacagcaaga agacggtttc ttcgataagt ttaaagatgc ttttggcggg
    19081 aaaaagcgga agtagttttt ctagtaaaaa cgctaagtca ttaataatta aaaaccagac
    19141 gaacaatcat atgttcgcct ggtttttttg tagttgtcat gtttgtacca acaaaaaagc
    19201 cagctttaga atatgataat cgtcatagtt tctaggggta taaccagtga gagaatggaa
    19261 cttgttcaat cgatattgca aggtgttttt atgaataaat aattcttctg agcaattttt
    19321 gatgcttccg ttatttttac tgaagcatgc caaagtcttc ttgaacaaat gtacttcttc
    19381 ttctgtcagg cttttcaaaa tacgttttac atataatgtt cgttgatctg acgggattgc
    19441 tggtaaaacg atcccgaggt ctaaatcttc aaaaaagcgg atctcgttca cagattgatg
    19501 gaaagctatc cattcgcaaa cttgtttggc atgaaggaaa gattgcggtg tctcttgctc
    19561 tgtataacaa agcgacccaa ttccaaagtt tagctgtaac tgatacattt tattaaactt
    19621 tgtctggatt ttatcaagca atgatagcaa tgaggctctg gaaatccgat tgactaggat
    19681 aatgatttgt tgtctatgta cagtgagcaa agcctgtccg taataagaga agaccctttc
    19741 gagcaactcc aataaaggat tgatccgttc ttgacacaat tgctgattgt cagaaagttg
    19801 tcccacaact acataatatg gagaagaaaa atcaagctca attaagtgag ggtggtatcg
    19861 actatgtact tgatatcatc aatgaaccga tggtactttc ttatgcgatg gaacaggtag
    19921 agcgattgct tgtttgtgca ggaaaacaag cgatgcggct gatacgatta ggtgagaaaa
    19981 taagaagata aaaatacacg gtcccttcat ggcgaccgtg catttttggt ttttattaaa
    20041 tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt tcaaagattt
    20101 gttgaggcgt tccatcttct aaaaagtttc cgtcgtcaat gaacatgacg cgattggcca
    20161 cttctttagc gaaccccatt tcgtgcgtaa cgataaccat cgtcatacct tgttctgcta
    20221 actttttcat gacgttcaag acatcaccga ccatttcagg atcgagtgca gaagtaggct
    20281 catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc acacgctgtt
    20341 tttgtccacc agaaagcatt tcaggataca tatctttttt atcttctaga ccaactgtct
    20401 gcagtaattt gattgctttt ttctctgctt cttctctaga ttctttcttc aggtccatcg
    20461 gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag tgttggaata
    20521 ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc aagtgggcgc
    20581 cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa cgcaagaatg
    20641 tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg ttgatggata
    20701 cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc tcaactaaga
    20761 ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt tgtcaatgct
    20821 gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact ttgtgttgta
    20881 cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc ggaaatgatc
    20941 gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat gatgcgtatc
    21001 gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa acttctggaa
    21061 gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc tgcgatataa
    21121 gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat cgtaaatcca
    21181 acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg aattcctcgg
    21241 atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc tacactgaat
    21301 aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt taggagaatt
    21361 gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc aagaaatgtt
    21421 gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac atattcttta
    21481 ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt gaacatatta
    21541 agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc agattctttt
    21601 ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac cggataatca
    21661 tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc agcagcatca
    21721 aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc agcactttcg
    21781 gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt gatcgaagta
    21841 tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga aaaatcatag
    21901 cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc ttgtcctgat
    21961 tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt aaatccttgc
    22021 agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc ctttgcgttt
    22081 tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc ttttttgggc
    22141 tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt gtcagcttgt
    22201 gcaacatgac caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa tgagtgaaat
    22261 aaatgctttc ttttcataat caacctccta aatttccaat attttattat tataggcgaa
    22321 aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg agtttctaac
    22381 attttatgtt ataaaacata acatgtataa taagataaaa aagtggcgaa agtatgttcg
    22441 gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt attggtcgta
    22501 aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat acctcccgac
    22561 actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg attaaccaat
    22621 tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg actggaacag
    22681 ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca ctgattattg
    22741 cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt ttccctaaca
    22801 atgctgttga atattttgtt tcttattatg attattatca gcctgaagct tatgtgcctt
    22861 caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac aaattacgac
    22921 attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca tccgtttcat
    22981 gtatttttgg attaggctcg ccttttgagt accaaaaaca agtagtttcg attcgacagg
    23041 gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa ttcgaaagaa
    23101 atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg gagatttttc
    23161 cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag gtcgaacgta
    23221 tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac gtagcaatct
    23281 tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta gcgaatatca
    23341 aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt ctggaagctc
    23401 aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg ggctacacgt
    23461 cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa ccgccttata
    23521 cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg cacgtaacca
    23581 tgcctcaaat cagaggaatg tataatggtg accgagcgag aaaacagatg ctagtagatt
    23641 acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa gagtttgaaa
    23701 aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa catgaacaaa
    23761 cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca gtgatcgaag
    23821 ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa cgagtcgaaa
    23881 aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac ctcacagatt
    23941 atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa acgctggaac
    24001 gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt ggaatcaatt
    24061 tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg gatgcggata
    24121 aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga gctgctcgga
    24181 atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg cgtctagcga
    24241 tggatgaaac gtcaagacgt cgtacgattc agcagaagta caatgaagaa catgggatcg
    24301 ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa gaatcggaag
    24361 atgatacaaa agaagcggta caagtctcgt acgaagaaat gacgaaggaa gaaaaagaca
    24421 cgttattgat gaaattagaa aaagaaatga aagatgcggc aaaagctttg gattttgaaa
    24481 cagccgcgaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag gaggacaaca
    24541 tggctaacga taaaattacg attcatggtg cacgtgccca taacttgaaa aatatagatg
    24601 taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct ggaaaaagtt
    24661 ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag agcctttctg
    24721 cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc atcgacggat
    24781 tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg tcgacagtag
    24841 gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta ggacatccga
    24901 tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg gcagatcaag
    24961 taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg gtaaagaaaa
    25021 aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc cgcgttcgag
    25081 tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac aaaaaacatg
    25141 atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct cgcctatttg
    25201 attctttaga ggcggccctg cgattagcag acggctatgc tctggttgac gtgatcggtg
    25261 aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt actgttggcg
    25321 aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca gactgtgatg
    25381 gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga acgaagacac
    25441 ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat cctcaaatgt
    25501 tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa gaattaccgg
    25561 aagagcatca ggagatcatc ttgaatggga cagatgatct tttccatttc cattatgaaa
    25621 atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg aagaatatcg
    25681 agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt ctttatatga
    25741 cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct ctatctgttc
    25801 aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa gcagtagatt
    25861 tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg attttaaaag
    25921 aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta actttgagtc
    25981 gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct acacaaattg
    26041 gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga ttgcatcaac
    26101 gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc aacaccctcg
    26161 ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatttgatt gacgtcggac
    26221 ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa gaagtagcta
    26281 atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc cctgtaccaa
    26341 aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca gaaaacaatc
    26401 tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca ggtgtgtctg
    26461 gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg caaaaattaa
    26521 ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa agtatcgaaa
    26581 agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat ccagcgactt
    26641 atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag gcaaaaatgc
    26701 ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt gaagcttgtc
    26761 gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat gttccttgtg
    26821 aagtatgtca tggtaaacga tataattctg aaacattaga agtgcattac aaaggaaaaa
    26881 gcattgctga tattttggaa atgacagtag aggatgctgt agaattcttc aagcacattc
    26941 caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat gtgacgatgg
    27001 ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt gccagtgaat
    27061 tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg acaggacttc
    27121 atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat gctggtaaca
    27181 cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat atcattgatt
    27241 taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca ccagaagaaa
    27301 tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg gtataaagag
    27361 gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt gttttattaa
    27421 tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt atagataaaa
    27481 acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggac tttcctttga
    27541 ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa acaagagatt
    27601 atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat ccctatatta
    27661 ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc atacggcaaa
    27721 atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa aattgtcaat
    27781 gtagaagtta ttccaggatt tgataaagac cctgtattta tcgagacgat gcttcgtttg
    27841 gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg tggtgatgga
    27901 tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat ctgtccatat
    27961 attgattatt ctttatttga aaggctgatc aataaagtaa gcttttatga agtttgtgaa
    28021 gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt agtaaatgga
    28081 catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc aaacagtgtg
    28141 gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga gactagagaa
    28201 gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga aatgattgta
    28261 caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta tgtagatgaa
    28321 gatcatcaag ttcggatgat gtgccttggt catccactat tagaagatcc tacaccagca
    28381 tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta tcaaaccatt
    28441 aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat gaagtatgat
    28501 cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg tagcagtttc
    28561 tttgtgacat tgaacggctt gaatctggct cgttttgtca cagaagatcg agtgttcaac
    28621 aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag attatggatg
    28681 ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa aaaactagct
    28741 gagcaaatga tcaaagaaaa tcgttacggt actactgtat tttatgaaaa agaccgatca
    28801 atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag attcaaaaag
    28861 tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt aggcggaatg
    28921 ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa agctacaaaa
    28981 gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga tcgtacagct
    29041 tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga tattgagaaa
    29101 caaaatttat tgcgaccaaa cttcattgtg ttgacttgta acaccgcaca ctactttttt
    29161 gaagagttac aagcggcaac ggatattcct atcttgcata tgccaagaga agcagcaaat
    29221 gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga aggaagtatg
    29281 aaagctggaa tctatgaacg cgaagtcaaa aatcttggtt tcgaaacagt gattcctgat
    29341 actgcgcttc aagaaaaaat caattacctg atctatcatg agatcaagga atcggattat
    29401 ttgaatcaag agctctatta cgaaatctta gaggaagcgg tagagcgcct aaactgtgaa
    29461 aaagtcatcc taggttgcac agaattatct ttgatgcatg aatttgctga agataatcat
    29521 tacccagtga tcgatgctca atctatttta gcggatcgaa cgatcgaacg tgcattagct
    29581 gaaagaagcg aggcattgga tacagcatcg gaaaagtaaa aaaattggat ttggggcaaa
    29641 tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac tggtaatgat
    29701 acgcccggga tgatataatt ttattatgca caatgtgaga ggggttcgta aatatgacag
    29761 ataatttgca attagtcatc ataacaggta tgagtggtgc agggaaaaca gtagctatcc
    29821 aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga ttgattccaa
    29881 aattctggga attgatcaaa gaatcaggta aagtcacaaa aatggcttta ggagtggatt
    29941 tgcgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc gaaaatacga
    30001 acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta gtggctcgct
    30061 acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact gaagggatac
    30121 gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc attgatacga
    30181 caacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa tcaaagggag
    30241 accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg ccgatcgatt
    30301 cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg gaactaagac
    30361 cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat gaaacgcaat
    30421 cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac attaaagaag
    30481 gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt tctgtggcat
    30541 tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg acacatcggg
    30601 acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct accggataag
    30661 aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc tgaaaagttt
    30721 acgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg gtggaagcag
    30781 cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa atgtcttagt
    30841 ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt tcaaaaagga
    30901 agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag tttccgagat
    30961 gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg tagatggaca
    31021 tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtttttaaag acggaacgac
    31081 tgcagtagga gaatctaaaa tcgcgatcaa ccgtaaaacc attgaccgtg tgtatgtctg
    31141 caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag caattatgga
    31201 cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc caaatttagt
    31261 gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct atatctgtaa
    31321 tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata tacgtgtttt
    31381 acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg aaaaagtgcc
    31441 tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg cacacgactt
    31501 caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttag aattgaaaga
    31561 tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt tagttttcgg
    31621 ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt catttgctgc
    31681 agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga aagcggaatt
    31741 agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt ttattttgaa
    31801 cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga aagatcatta
    31861 tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga acaatgtcta
    31921 tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata tcatggatgg
    31981 tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa aaatgcgttc
    32041 ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa cgagtcgtta
    32101 tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg atatgttaaa
    32161 ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt cgtatttaaa
    32221 aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact caatgctccg
    32281 atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc tagtcaattg
    32341 tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg aaaacatcga
    32401 attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg aaatcgcaga
    32461 acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga ttccctcagg
    32521 tgcaatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat ttgcggaaaa
    32581 actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca ggagatgcga
    32641 atgtatcagt tttttttaaa agctatactt gcttttcttc cgtcaaacta taattccaac
    32701 tttactggag taacataatg ttgaaaggct tctattgttt tttaatacaa tcgtataaaa
    32761 agcggggaga caactgaatg aagattttag ttttatacat aaacttttgc ttaatataga
    32821 agtcctgata tagataggat caagtattta tctataactg gagattaatt aaaaactgga
    32881 aggaactgct cgatcaacct tgagaactgt ttttcttact tctggtcaag attctcgtgg
    32941 ttggtggata aggatggttg ataagttcat tggattagct agtcgaaaac aaccaggtgt
    33001 aattaatgta gaagttttaa caaggggatg gagtgattta ttcagtgata atcataagtt
    33061 cataaatagt aattgggatg tatggttggt atacgttata cctgaacttg aactatcaag
    33121 atttggttta gaatttgaga atcgatgttt ataaagggag ggcaaaaaat gtgctttttt
    33181 acaactttta ctatcattat gattgcagta ctttttattt ttctagatat agcaaagcga
    33241 aatacagcat ttttattata tcgggtttta cttcgagcag gtctcataac atttatatcg
    33301 atcgttggat tttttctatt cacagttatt gtatttattt ggagaactac agcaccacca
    33361 ttgccagaga ttacatatgg agaatttccg tttagtcttg aatatgaaat agatgaggaa
    33421 agaattttga ttgaagatac ggtgattgca gaatttatag gaagttttag agggaatgtg
    33481 acaactcaac ctagacgaag ttggagtata aaacttttaa ataatagtgg agagttgaaa
    33541 gcatttcatt atgaattctt aataatagaa agtgaagatg taacaatcac tttaagtagt
    33601 ggctctgctg gatattttat gaatgatggt ggaagcaagc cacagctcac agtccgtgat
    33661 cgtattgcag gttttatgca tttttctcac tatgacttag aagaagctcg taaaatactt
    33721 gcagaatatg ggatcacatt aatcaattgg gaatatgcgc caccgataag aaattttttc
    33781 aatgattatt attagtctta taaaaaacag aatgtaaaat ttatgtgcgc gagtatttag
    33841 ttttccattt tttgtttgat agatatatag cattgttgaa gcatgggata gtggtgccaa
    33901 ttttatcaat gaggttggtc aggcagttaa cataattggt gatgctataa atccatttaa
    33961 ttggaattgg aggaattagg tgtggaaaca gtgagattat ggatattcat gaaaaatttt
    34021 cgttcggctt tgagatatgt tattctggaa tcaaaatcag gttattatct gattgacaca
    34081 gatcactata ctttttgggg aggctacctt tttccgattt ttaattggat aatcccccat
    34141 aaggttatga aaattaactt gcctgcagaa gaaataaata gattaatact caacaaagat
    34201 ttacaaagga aaagagagaa agaacattct aatgttgctt ggataggtcc agcatttgca
    34261 gtagtgactt cttcaattgt cggtcctatg ctagagagta tttatagtga tttttctatt
    34321 ggaatacgtt atttatttgt tactttaagt atagctgttc ttttgtgcat aaagggttat
    34381 gttagcaaga agaataaaga tattttaaca ctaatcggtg taaacaattt atctagtact
    34441 aaaattatac tgtttcctga atctataaaa gaaggatcaa ggttgttgat aatttttata
    34501 atgcttatac tgttttctgt tttatttggg gtgatgttta taga
//
LOCUS       DAZKSR010000018        34393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000018 DAZKSR010000000
VERSION     DAZKSR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.018"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1936)
                     /locus_tag="ACG8XG_000771"
     CDS             complement(44..1936)
                     /locus_tag="ACG8XG_000771"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772707.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="ACG8XG_000772"
     CDS             complement(1933..3660)
                     /locus_tag="ACG8XG_000772"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772708.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="ACG8XG_000773"
     CDS             complement(3676..4326)
                     /locus_tag="ACG8XG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0772709.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="ACG8XG_000774"
     CDS             4530..4826
                     /locus_tag="ACG8XG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0772710.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="ACG8XG_000775"
     CDS             5018..5638
                     /locus_tag="ACG8XG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0772711.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="ACG8XG_000776"
     CDS             complement(5730..6896)
                     /locus_tag="ACG8XG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0772712.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="ACG8XG_000777"
     CDS             complement(6908..7261)
                     /locus_tag="ACG8XG_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0772713.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="ACG8XG_000778"
     CDS             7389..8411
                     /locus_tag="ACG8XG_000778"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0772714.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="ACG8XG_000779"
     CDS             8430..8591
                     /locus_tag="ACG8XG_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772715.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="ACG8XG_000780"
     CDS             complement(8850..9284)
                     /locus_tag="ACG8XG_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772716.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="ACG8XG_000781"
     CDS             complement(9394..11253)
                     /locus_tag="ACG8XG_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0772717.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11254..11394
                     /locus_tag="ACG8XG_000782"
     CDS             11254..11394
                     /locus_tag="ACG8XG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0772718.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11371..12531)
                     /locus_tag="ACG8XG_000783"
     CDS             complement(11371..12531)
                     /locus_tag="ACG8XG_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0772719.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="ACG8XG_000784"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="ACG8XG_000784"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0772720.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="ACG8XG_000785"
     CDS             complement(14278..14976)
                     /locus_tag="ACG8XG_000785"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0772721.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="ACG8XG_000786"
     CDS             complement(14933..16594)
                     /locus_tag="ACG8XG_000786"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0772722.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="ACG8XG_000787"
     CDS             16812..18212
                     /locus_tag="ACG8XG_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0772723.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="ACG8XG_000788"
     CDS             complement(18464..19543)
                     /locus_tag="ACG8XG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0772724.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="ACG8XG_000789"
     CDS             complement(19629..20588)
                     /locus_tag="ACG8XG_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0772725.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="ACG8XG_000790"
     CDS             complement(20600..22111)
                     /locus_tag="ACG8XG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0772726.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..22697)
                     /locus_tag="ACG8XG_000791"
                     /pseudo
     CDS             complement(22133..22697)
                     /locus_tag="ACG8XG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(22703..23566)
                     /locus_tag="ACG8XG_000792"
     CDS             complement(22703..23566)
                     /locus_tag="ACG8XG_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772727.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23580..24476)
                     /locus_tag="ACG8XG_000793"
     CDS             complement(23580..24476)
                     /locus_tag="ACG8XG_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772728.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24651..25940)
                     /locus_tag="ACG8XG_000794"
     CDS             complement(24651..25940)
                     /locus_tag="ACG8XG_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0772729.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25912..27429)
                     /locus_tag="ACG8XG_000795"
     CDS             complement(25912..27429)
                     /locus_tag="ACG8XG_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0772730.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27627..28517
                     /locus_tag="ACG8XG_000796"
     CDS             27627..28517
                     /locus_tag="ACG8XG_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0772731.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="ACG8XG_000797"
     CDS             complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="ACG8XG_000797"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0772732.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29426..30898)
                     /locus_tag="ACG8XG_000798"
     CDS             complement(29426..30898)
                     /locus_tag="ACG8XG_000798"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0772733.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31535..31897)
                     /locus_tag="ACG8XG_000799"
     CDS             complement(31535..31897)
                     /locus_tag="ACG8XG_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0772734.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32120..32872
                     /locus_tag="ACG8XG_000800"
     CDS             32120..32872
                     /locus_tag="ACG8XG_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0772735.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33012..33935)
                     /locus_tag="ACG8XG_000801"
     CDS             complement(33012..33935)
                     /locus_tag="ACG8XG_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772736.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33950..34222)
                     /locus_tag="ACG8XG_000802"
     CDS             complement(33950..34222)
                     /locus_tag="ACG8XG_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772737.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10148 a         7166 c         5911 g        11168 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa
    22381 gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa
    22441 caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct
    22501 ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca
    22561 ttacataaaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22621 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    22681 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22741 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22801 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    22861 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    22921 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    22981 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23041 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23101 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23161 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23221 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23281 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23341 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23401 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23461 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23521 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23581 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23641 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23701 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23761 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    23821 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    23881 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    23941 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24001 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24061 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24121 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24181 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24241 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24301 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24361 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24421 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24481 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24541 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24601 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24661 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24721 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24781 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    24841 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    24901 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    24961 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25021 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25081 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25141 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25201 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25261 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25321 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25381 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25441 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25501 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25561 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25621 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25681 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25741 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25801 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    25861 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    25921 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    25981 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26041 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26101 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26161 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26221 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26281 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26341 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    26401 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26461 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26521 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26581 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26641 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26701 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26761 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    26821 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    26881 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    26941 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27001 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27061 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27121 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27181 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27241 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27301 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27361 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27421 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27481 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    27541 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27601 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27661 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27721 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27781 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    27841 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    27901 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    27961 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28021 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28081 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28141 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28201 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28261 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28321 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28381 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28441 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28501 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28561 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28621 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28681 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28741 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28801 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    28861 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    28921 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    28981 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29041 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29101 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29161 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29221 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29281 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29341 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29401 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29461 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29521 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29581 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29641 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29701 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29761 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    29821 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    29881 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    29941 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30001 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30061 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30121 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30181 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30241 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30301 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30361 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30421 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30481 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30541 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30601 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30661 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30721 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30781 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    30841 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    30901 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    30961 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31021 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31081 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31141 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31201 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31261 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31321 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31381 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31441 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31501 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31561 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31621 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31681 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31741 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31801 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    31861 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    31921 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    31981 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32041 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32101 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32161 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32221 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32281 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32341 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32401 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32461 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32521 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32581 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32641 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32701 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    32761 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    32821 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    32881 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    32941 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33001 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33061 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33121 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33181 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33241 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33301 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33361 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33421 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33481 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33541 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33601 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    33661 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33721 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33781 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    33841 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    33901 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    33961 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34021 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34081 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34141 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34201 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34261 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34321 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    34381 attattagtg aat
//
LOCUS       DAZKSR010000019        34323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000019 DAZKSR010000000
VERSION     DAZKSR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.019"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8XG_000803"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACG8XG_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0772738.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACG8XG_000804"
     CDS             499..861
                     /locus_tag="ACG8XG_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0772739.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACG8XG_000805"
     CDS             913..2595
                     /locus_tag="ACG8XG_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0772740.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDDIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACG8XG_000806"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACG8XG_000806"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0772741.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8XG_000807"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACG8XG_000807"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0772742.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8XG_000808"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACG8XG_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0772743.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="ACG8XG_000809"
     CDS             6430..7434
                     /locus_tag="ACG8XG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772744.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="ACG8XG_000810"
     CDS             7435..8379
                     /locus_tag="ACG8XG_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772745.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /gene="opp4B"
                     /locus_tag="ACG8XG_000811"
     CDS             8379..9341
                     /gene="opp4B"
                     /locus_tag="ACG8XG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0772746.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="ACG8XG_000812"
     CDS             9368..10282
                     /locus_tag="ACG8XG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772747.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="ACG8XG_000813"
     CDS             10308..12089
                     /locus_tag="ACG8XG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0772748.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /gene="rnc"
                     /locus_tag="ACG8XG_000814"
     CDS             12437..13123
                     /gene="rnc"
                     /locus_tag="ACG8XG_000814"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0772749.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="ACG8XG_000815"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="ACG8XG_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0772750.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="ACG8XG_000816"
     CDS             16733..17542
                     /locus_tag="ACG8XG_000816"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0772751.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="ACG8XG_000817"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="ACG8XG_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0772752.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="ACG8XG_000818"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="ACG8XG_000818"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0772753.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="ACG8XG_000819"
     CDS             complement(21094..21546)
                     /locus_tag="ACG8XG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0772754.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="ACG8XG_000820"
     CDS             21696..22646
                     /locus_tag="ACG8XG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0772755.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="ACG8XG_000821"
     CDS             22639..23598
                     /locus_tag="ACG8XG_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0772756.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="ACG8XG_000822"
     CDS             23595..24350
                     /locus_tag="ACG8XG_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772757.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="ACG8XG_000823"
     CDS             24373..25326
                     /locus_tag="ACG8XG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0772758.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="ACG8XG_000824"
     CDS             complement(25970..26266)
                     /locus_tag="ACG8XG_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0772759.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="ACG8XG_000825"
     CDS             26621..26977
                     /locus_tag="ACG8XG_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0772760.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="ACG8XG_000826"
     CDS             26987..27886
                     /locus_tag="ACG8XG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0772761.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28406..29134
                     /locus_tag="ACG8XG_000827"
     CDS             28406..29134
                     /locus_tag="ACG8XG_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0772762.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="ACG8XG_000828"
     CDS             29279..31306
                     /locus_tag="ACG8XG_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0772763.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="ACG8XG_000829"
     CDS             31414..32511
                     /locus_tag="ACG8XG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0772764.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..34287
                     /locus_tag="ACG8XG_000830"
     CDS             32818..34287
                     /locus_tag="ACG8XG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772765.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTGNPKNIRLFL
                     YNLFNSTEISFVELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAEQAD
                     SLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETI
                     EWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFREN
                     YPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSY
                     GAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRA
                     VDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT        11518 a         5922 c         7299 g         9584 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tgatatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gttgacaggg aatcctaaaa acattcggct ttttttatat
    33301 aatcttttta actcaacaga gataagtttt gtggaattgt atacgctacc tataattata
    33361 aaaacagaaa aaatgttaaa agatttaaaa cagtattatc ctctgtctgc ttatgaaaaa
    33421 aagaaattat tccacttttt agcaatcaac ctgttggctg aacaagctga ttctttagat
    33481 attgctagca atcagtcgct atttttttcg gatataggat tttaccactt aaaaggagta
    33541 ttaggagaaa aatccgttat ttttatcaat aagacactct cttggcttta tcttcatgat
    33601 aaattggatc aaaaatactt gagaaaaaat gaagatgaaa caatcgaatg gttgaatgaa
    33661 cgattcattg aaagttttga aaatcagttt aatctgttgc gagaagagac gagccaattg
    33721 atccgaagag agttaaccaa aatacatttt aaacttttgc attacactat taataaagct
    33781 gatgaatttc cacttgatgt ttcttttttt agagaaaact atccagcatt ttattgctat
    33841 ttgatagagt atataaatga tctaacgtca aaacgcaaag agttgcttca agataaattt
    33901 tttctttttt tcagttattt attattattg atcaatcaca tacccattca actgatttct
    33961 gaaccagtaa agatcatcat cgatttttct tatggtgctg cctataatca atttatcaag
    34021 aaaaatttaa gcttgtacat taatctgaac acggaagtga tagaggcagg aaagcctgag
    34081 ttcccggatg ttataatcac gaataggaat aatctatatg aagaaagtgc atctcaagta
    34141 gtggtttggc tcgatccgcc aagagcagta gactggggga atcttacaaa gattttgttg
    34201 actatccaag aagagaaata caaagacagc aaaattgaca agcaaataga cgattttcct
    34261 gaagaaatca tcgacaggat cgaatagtga acttctttta gaaagtccga ctgctttttc
    34321 taa
//
LOCUS       DAZKSR010000020        33489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000020 DAZKSR010000000
VERSION     DAZKSR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.020"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..537
                     /locus_tag="ACG8XG_000831"
                     /pseudo
     CDS             49..537
                     /locus_tag="ACG8XG_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(626..1018)
                     /gene="rpsI"
                     /locus_tag="ACG8XG_000832"
     CDS             complement(626..1018)
                     /gene="rpsI"
                     /locus_tag="ACG8XG_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0772766.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1034..1462)
                     /gene="rplM"
                     /locus_tag="ACG8XG_000833"
     CDS             complement(1034..1462)
                     /gene="rplM"
                     /locus_tag="ACG8XG_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0772767.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(1996..2463)
                     /locus_tag="ACG8XG_000834"
     CDS             complement(1996..2463)
                     /locus_tag="ACG8XG_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0772768.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2583..4121)
                     /locus_tag="ACG8XG_000835"
     CDS             complement(2583..4121)
                     /locus_tag="ACG8XG_000835"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0772769.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4201..4887
                     /locus_tag="ACG8XG_000836"
     CDS             4201..4887
                     /locus_tag="ACG8XG_000836"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0772770.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5088..5615)
                     /gene="msrA"
                     /locus_tag="ACG8XG_000837"
     CDS             complement(5088..5615)
                     /gene="msrA"
                     /locus_tag="ACG8XG_000837"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0772771.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5699..6376)
                     /gene="pgmB"
                     /locus_tag="ACG8XG_000838"
     CDS             complement(5699..6376)
                     /gene="pgmB"
                     /locus_tag="ACG8XG_000838"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0772772.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6373..7501)
                     /locus_tag="ACG8XG_000839"
                     /pseudo
     CDS             complement(6373..7501)
                     /locus_tag="ACG8XG_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7501..9684)
                     /locus_tag="ACG8XG_000840"
     CDS             complement(7501..9684)
                     /locus_tag="ACG8XG_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0772773.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9681..10706)
                     /locus_tag="ACG8XG_000841"
     CDS             complement(9681..10706)
                     /locus_tag="ACG8XG_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0772774.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10751..11578)
                     /locus_tag="ACG8XG_000842"
     CDS             complement(10751..11578)
                     /locus_tag="ACG8XG_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0772775.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11575..12642)
                     /locus_tag="ACG8XG_000843"
     CDS             complement(11575..12642)
                     /locus_tag="ACG8XG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0772776.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12668..13504)
                     /locus_tag="ACG8XG_000844"
     CDS             complement(12668..13504)
                     /locus_tag="ACG8XG_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772777.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13501..14370)
                     /locus_tag="ACG8XG_000845"
     CDS             complement(13501..14370)
                     /locus_tag="ACG8XG_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0772778.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14440..15723)
                     /locus_tag="ACG8XG_000846"
     CDS             complement(14440..15723)
                     /locus_tag="ACG8XG_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0772779.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15737..17422)
                     /locus_tag="ACG8XG_000847"
     CDS             complement(15737..17422)
                     /locus_tag="ACG8XG_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0772780.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17697..18722
                     /locus_tag="ACG8XG_000848"
     CDS             17697..18722
                     /locus_tag="ACG8XG_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0772781.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19317..22970)
                     /gene="rpoC"
                     /locus_tag="ACG8XG_000849"
     CDS             complement(19317..22970)
                     /gene="rpoC"
                     /locus_tag="ACG8XG_000849"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0772782.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23049..26666)
                     /gene="rpoB"
                     /locus_tag="ACG8XG_000850"
     CDS             complement(23049..26666)
                     /gene="rpoB"
                     /locus_tag="ACG8XG_000850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0772783.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27394..28362
                     /locus_tag="ACG8XG_000851"
     CDS             27394..28362
                     /locus_tag="ACG8XG_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0772784.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28380..29285
                     /locus_tag="ACG8XG_000852"
     CDS             28380..29285
                     /locus_tag="ACG8XG_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772785.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29282..30088
                     /locus_tag="ACG8XG_000853"
     CDS             29282..30088
                     /locus_tag="ACG8XG_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772786.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30245..31213
                     /locus_tag="ACG8XG_000854"
     CDS             30245..31213
                     /locus_tag="ACG8XG_000854"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0772787.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31530..32054)
                     /locus_tag="ACG8XG_000855"
     CDS             complement(31530..32054)
                     /locus_tag="ACG8XG_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0772788.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32341..>33489
                     /gene="scm"
                     /locus_tag="ACG8XG_000856"
     CDS             32341..>33489
                     /gene="scm"
                     /locus_tag="ACG8XG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0772789.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTT"
BASE COUNT         9456 a         7196 c         6290 g        10547 t
ORIGIN      
        1 aacatgttta gacattttcc tctaggaaaa aattaactag caccacgggt tgattttaat
       61 aaaaatacgg ttcgtctcaa atatgcttta gataaatatc aaagaaagac acatacaaaa
      121 ggttggaaag acaccgttat tcaggcacca gaagttgtga tgacattatt aagagaatgg
      181 aaatcagctc aggctgaaca attgttgaaa ctaaaaatta tacaaacacc tgaccagtac
      241 ctctttacct attccaaacc ttctggagaa gtaaattgtc ctgttcacgc tgactactta
      301 aactatcgaa tcaatgcaat aaaaaaaagg catcctgaat tggctcaatt atctccacac
      361 aaacttcgtc atacctatgc aactatagct agggaaggtg gagcaaatat gaatcaaatc
      421 tctaatgctt taacgcactc ggatatttct actacaaaaa tatacgtgaa cacacctgac
      481 gtagttgata aatctgtttt tgaagcattt caaaaaggat tgaataagtg tgattaacat
      541 gtgttctcaa atgtgttctt attataaaaa aagacacttt ccaaataatt gaaaagtgtc
      601 ctgaaatatt gatataaaaa gaatattaac gttttgagaa ttgtgaagct ttacgagctt
      661 ttttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac
      721 gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac
      781 ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg
      841 cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg
      901 gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac
      961 gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg
     1021 aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat
     1081 gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt
     1141 ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca
     1201 attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt
     1261 tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat
     1321 cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa
     1381 caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt
     1441 ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt
     1501 ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt
     1561 aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat
     1621 atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa
     1681 aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt
     1741 ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt
     1801 aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg
     1861 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg
     1921 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
     1981 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
     2041 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
     2101 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
     2161 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
     2221 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
     2281 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
     2341 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
     2401 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
     2461 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
     2521 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
     2581 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
     2641 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
     2701 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
     2761 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
     2821 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
     2881 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     2941 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     3001 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     3061 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     3121 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     3181 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     3241 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     3301 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     3361 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     3421 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     3481 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     3541 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     3601 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     3661 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     3721 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     3781 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     3841 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     3901 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     3961 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     4021 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     4081 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     4141 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     4201 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     4261 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     4321 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     4381 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     4441 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     4501 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     4561 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     4621 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     4681 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     4741 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     4801 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     4861 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     4921 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     4981 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     5041 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     5101 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     5161 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     5221 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     5281 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     5341 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     5401 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     5461 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     5521 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     5581 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     5641 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     5701 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     5761 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     5821 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     5881 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     5941 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     6001 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     6061 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     6121 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     6181 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     6241 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     6301 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     6361 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     6421 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     6481 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     6541 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     6601 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     6661 gaggtctgct tgctgtaccc gtatcgaact ccagtaaatc caatacgtag tcgatgccag
     6721 agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac
     6781 tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt
     6841 cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc
     6901 cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca
     6961 gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg
     7021 cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga
     7081 tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt
     7141 gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt
     7201 ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac
     7261 cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt
     7321 gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta
     7381 taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt
     7441 gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca
     7501 ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac
     7561 aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac
     7621 aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg
     7681 aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat
     7741 aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg
     7801 atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc
     7861 ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt
     7921 tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa
     7981 taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct
     8041 acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc
     8101 aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac
     8161 tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc
     8221 ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt
     8281 gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc
     8341 gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt
     8401 tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag
     8461 gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt
     8521 gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg
     8581 gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc
     8641 ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag
     8701 gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt
     8761 cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc
     8821 gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg
     8881 ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt
     8941 ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac
     9001 gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt
     9061 gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat
     9121 atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg
     9181 cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt
     9241 tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg
     9301 atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc
     9361 agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta
     9421 tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct
     9481 gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac
     9541 accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat
     9601 accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc
     9661 aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat
     9721 aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc
     9781 tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact
     9841 ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga
     9901 tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata
     9961 ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca
    10021 aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt
    10081 tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca
    10141 gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct
    10201 tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc
    10261 gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg
    10321 tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct
    10381 gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca
    10441 ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattggctgt acagacactc
    10501 acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat
    10561 cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat
    10621 tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca
    10681 gcgcctatga ttgctacttt cttcatttat ttctctctcc tttgcttatt tacttattgt
    10741 tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt
    10801 tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt
    10861 catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat
    10921 caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat
    10981 actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa
    11041 aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg
    11101 ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac
    11161 ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc
    11221 cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg
    11281 ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt
    11341 gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt
    11401 tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat
    11461 tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc
    11521 taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg
    11581 ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata
    11641 acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca
    11701 atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca
    11761 tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt
    11821 gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata
    11881 agaaatgatc ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc
    11941 actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc
    12001 gacatctgct ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct
    12061 acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg
    12121 aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac
    12181 atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt
    12241 ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag
    12301 cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa
    12361 actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac
    12421 cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc
    12481 ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt
    12541 agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt
    12601 gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct
    12661 tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata
    12721 acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc
    12781 aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg
    12841 aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa
    12901 cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga
    12961 tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat
    13021 aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg
    13081 ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca
    13141 aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga
    13201 agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg
    13261 ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat
    13321 tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg
    13381 caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat
    13441 tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt
    13501 tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa
    13561 tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa
    13621 ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa
    13681 catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt
    13741 cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt
    13801 tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca
    13861 gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc
    13921 ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat aactattatt
    13981 gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat
    14041 cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa
    14101 tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga
    14161 acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc
    14221 gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg
    14281 aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt
    14341 taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag
    14401 ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc
    14461 gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc
    14521 gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc
    14581 aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt
    14641 gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc
    14701 accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt
    14761 gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga
    14821 tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga
    14881 gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt
    14941 tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg
    15001 ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc
    15061 gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc
    15121 actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt
    15181 gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc
    15241 tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat
    15301 atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc
    15361 tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat
    15421 cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa
    15481 cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc
    15541 tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc
    15601 gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc
    15661 accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt
    15721 catttttttc cacctcttat tttattattt tcagccacat atattcatac ggctggaata
    15781 tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc
    15841 cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact aagactttct
    15901 cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca
    15961 aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac
    16021 catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa
    16081 atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc
    16141 gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg
    16201 aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg
    16261 catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt
    16321 agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg
    16381 gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa
    16441 aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat
    16501 tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg
    16561 cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag
    16621 tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt
    16681 tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag
    16741 cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt
    16801 tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc
    16861 atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg
    16921 gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg
    16981 tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga
    17041 caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca
    17101 aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga
    17161 aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct
    17221 taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga
    17281 aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac
    17341 tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg
    17401 cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat
    17461 ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata
    17521 cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat
    17581 ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg
    17641 tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga
    17701 aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag
    17761 tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca
    17821 aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg
    17881 cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag
    17941 atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa
    18001 cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga
    18061 tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt gaattggatc
    18121 aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact
    18181 cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg
    18241 gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc
    18301 tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta
    18361 tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac
    18421 gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag
    18481 tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc
    18541 ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt
    18601 taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa
    18661 aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt
    18721 agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt
    18781 caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat
    18841 ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga
    18901 agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac
    18961 atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa
    19021 taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa
    19081 atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta
    19141 tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat
    19201 acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa attattttag
    19261 acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat
    19321 tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg
    19381 tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata
    19441 ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga
    19501 tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca
    19561 gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga
    19621 gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg
    19681 atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta
    19741 cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata
    19801 cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact
    19861 tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata
    19921 tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt
    19981 tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca
    20041 tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct
    20101 tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta
    20161 cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct
    20221 tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca
    20281 ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca
    20341 acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt
    20401 gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat
    20461 tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa
    20521 tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct
    20581 gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata
    20641 aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt
    20701 cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg
    20761 ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc
    20821 atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa
    20881 cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta
    20941 tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact
    21001 gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta
    21061 gaagtttctt ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc
    21121 ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct
    21181 tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga
    21241 taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc
    21301 gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg
    21361 atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct
    21421 ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct
    21481 agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt
    21541 tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg
    21601 acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa
    21661 cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc
    21721 gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat
    21781 acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg
    21841 cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga
    21901 agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag
    21961 tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga
    22021 gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta
    22081 tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca
    22141 ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa
    22201 agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa
    22261 tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg
    22321 cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca
    22381 gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat
    22441 tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt
    22501 tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg
    22561 atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat
    22621 acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct
    22681 aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat
    22741 cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag
    22801 tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg ttttaaggta
    22861 cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg
    22921 ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc
    22981 ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat
    23041 agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt
    23101 tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca
    23161 aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt
    23221 tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct
    23281 ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg
    23341 attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct
    23401 tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt
    23461 tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg
    23521 attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca
    23581 ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc
    23641 gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca
    23701 tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat
    23761 tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg
    23821 attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc
    23881 cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg
    23941 atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta
    24001 aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg
    24061 cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat
    24121 tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca
    24181 ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg
    24241 atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct
    24301 tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca
    24361 tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc
    24421 atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc gactttctca
    24481 cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt
    24541 gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca
    24601 acgtattcaa cgacaccatc atgtttacat aacaaagcag ctcctgagtc atgcgctgat
    24661 ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga
    24721 cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata
    24781 catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca
    24841 atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt
    24901 ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct
    24961 gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt
    25021 gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg
    25081 atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg
    25141 tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggcgaaaaga
    25201 cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga
    25261 gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat caattgttga
    25321 ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt
    25381 gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt
    25441 cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc
    25501 attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt
    25561 ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca
    25621 gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca
    25681 ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca
    25741 acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga
    25801 cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa
    25861 cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt
    25921 tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt
    25981 gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt
    26041 tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc
    26101 ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc
    26161 caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact
    26221 tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc
    26281 ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct
    26341 tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca
    26401 gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca
    26461 taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca
    26521 aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc
    26581 aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt
    26641 ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga
    26701 ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt
    26761 ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc
    26821 gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc
    26881 cttttcttca tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc
    26941 aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat
    27001 gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga
    27061 aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca
    27121 actgaataac ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa
    27181 acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc
    27241 acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt
    27301 ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat
    27361 tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta
    27421 ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca
    27481 tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct
    27541 gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta
    27601 acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa
    27661 ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta
    27721 ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc
    27781 catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg
    27841 ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc
    27901 atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa
    27961 ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa
    28021 caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg
    28081 ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt
    28141 gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa
    28201 agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt
    28261 gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa
    28321 gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca
    28381 tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac
    28441 ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag
    28501 gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat
    28561 tgacagcttg tcttctaggc tttttaggga gtgcggcagc agggcttgtt tccggactgc
    28621 ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat
    28681 attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga
    28741 tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc
    28801 tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac
    28861 ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag
    28921 ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct
    28981 tgcttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg
    29041 gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc
    29101 tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac
    29161 tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt
    29221 ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca
    29281 aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa
    29341 cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat
    29401 catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc
    29461 tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga
    29521 acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt
    29581 aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg
    29641 tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg
    29701 gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat
    29761 tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc
    29821 agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga
    29881 gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag
    29941 tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca
    30001 gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa
    30061 agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc
    30121 caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt
    30181 cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga
    30241 tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc
    30301 cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa
    30361 agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc
    30421 ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt
    30481 gaaagtacaa acgatgatga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc
    30541 tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg
    30601 ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca
    30661 tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt
    30721 gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta
    30781 catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg
    30841 caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt
    30901 tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac
    30961 aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga
    31021 aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa
    31081 tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat
    31141 cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat
    31201 ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt
    31261 ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct
    31321 agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat
    31381 gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt
    31441 ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt
    31501 ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca
    31561 tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct
    31621 cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc
    31681 cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct
    31741 atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta
    31801 tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt
    31861 cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc
    31921 attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag
    31981 acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata
    32041 tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct
    32101 aggataccta cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc
    32161 atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc
    32221 aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt
    32281 ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa
    32341 atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat
    32401 ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat
    32461 ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat
    32521 aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa
    32581 aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa
    32641 aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa
    32701 tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga
    32761 tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat
    32821 attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc
    32881 actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat
    32941 ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg
    33001 atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc
    33061 aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat
    33121 gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat
    33181 ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt
    33241 actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat
    33301 cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc
    33361 gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc aagcgacagt
    33421 acagatacta cctctaccac aagtgatagc acggatacat cagcttcaag cgacagtaca
    33481 gatactacc
//
LOCUS       DAZKSR010000021        32909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000021 DAZKSR010000000
VERSION     DAZKSR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.021"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..874
                     /locus_tag="ACG8XG_000857"
     CDS             131..874
                     /locus_tag="ACG8XG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772790.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            880..1245
                     /locus_tag="ACG8XG_000858"
     CDS             880..1245
                     /locus_tag="ACG8XG_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772791.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            1261..1791
                     /locus_tag="ACG8XG_000859"
     CDS             1261..1791
                     /locus_tag="ACG8XG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772792.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            1953..2261
                     /locus_tag="ACG8XG_000860"
     CDS             1953..2261
                     /locus_tag="ACG8XG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772793.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            2392..2676
                     /locus_tag="ACG8XG_000861"
     CDS             2392..2676
                     /locus_tag="ACG8XG_000861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772794.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            2813..3613
                     /locus_tag="ACG8XG_000862"
     CDS             2813..3613
                     /locus_tag="ACG8XG_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772795.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            3650..3832
                     /locus_tag="ACG8XG_000863"
     CDS             3650..3832
                     /locus_tag="ACG8XG_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772796.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            3943..4260
                     /locus_tag="ACG8XG_000864"
     CDS             3943..4260
                     /locus_tag="ACG8XG_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772797.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            4263..4406
                     /locus_tag="ACG8XG_000865"
     CDS             4263..4406
                     /locus_tag="ACG8XG_000865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772798.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(4610..5089)
                     /locus_tag="ACG8XG_000866"
     CDS             complement(4610..5089)
                     /locus_tag="ACG8XG_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772799.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            5173..6546
                     /locus_tag="ACG8XG_000867"
     CDS             5173..6546
                     /locus_tag="ACG8XG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0772800.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            6569..7096
                     /locus_tag="ACG8XG_000868"
     CDS             6569..7096
                     /locus_tag="ACG8XG_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772801.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            7083..7646
                     /locus_tag="ACG8XG_000869"
     CDS             7083..7646
                     /locus_tag="ACG8XG_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772802.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            8068..8805
                     /locus_tag="ACG8XG_000870"
     CDS             8068..8805
                     /locus_tag="ACG8XG_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772803.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8822..9238)
                     /locus_tag="ACG8XG_000871"
     CDS             complement(8822..9238)
                     /locus_tag="ACG8XG_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772804.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9631..10878
                     /locus_tag="ACG8XG_000872"
     CDS             9631..10878
                     /locus_tag="ACG8XG_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0772805.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11146..13536
                     /locus_tag="ACG8XG_000873"
     CDS             11146..13536
                     /locus_tag="ACG8XG_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0772806.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13585..15420
                     /locus_tag="ACG8XG_000874"
     CDS             13585..15420
                     /locus_tag="ACG8XG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0772807.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15495..16352
                     /locus_tag="ACG8XG_000875"
     CDS             15495..16352
                     /locus_tag="ACG8XG_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0772808.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            16342..17451
                     /locus_tag="ACG8XG_000876"
     CDS             16342..17451
                     /locus_tag="ACG8XG_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0772809.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            17790..18590
                     /locus_tag="ACG8XG_000877"
     CDS             17790..18590
                     /locus_tag="ACG8XG_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772810.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18696..19484
                     /locus_tag="ACG8XG_000878"
     CDS             18696..19484
                     /locus_tag="ACG8XG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772811.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19716..20585
                     /locus_tag="ACG8XG_000879"
     CDS             19716..20585
                     /locus_tag="ACG8XG_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0772812.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            20605..20952
                     /locus_tag="ACG8XG_000880"
     CDS             20605..20952
                     /locus_tag="ACG8XG_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772813.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20965..22227
                     /locus_tag="ACG8XG_000881"
     CDS             20965..22227
                     /locus_tag="ACG8XG_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772814.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            22307..23683
                     /locus_tag="ACG8XG_000882"
     CDS             22307..23683
                     /locus_tag="ACG8XG_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0772815.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            23680..24309
                     /locus_tag="ACG8XG_000883"
     CDS             23680..24309
                     /locus_tag="ACG8XG_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772816.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            24321..24791
                     /locus_tag="ACG8XG_000884"
     CDS             24321..24791
                     /locus_tag="ACG8XG_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772817.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            24788..25270
                     /locus_tag="ACG8XG_000885"
     CDS             24788..25270
                     /locus_tag="ACG8XG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772818.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            25267..25929
                     /locus_tag="ACG8XG_000886"
     CDS             25267..25929
                     /locus_tag="ACG8XG_000886"
                     /inference="COORDINATES: protein motif:HMM:NF019467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0772819.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            25916..26284
                     /locus_tag="ACG8XG_000887"
     CDS             25916..26284
                     /locus_tag="ACG8XG_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0772820.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            26365..26817
                     /locus_tag="ACG8XG_000888"
     CDS             26365..26817
                     /locus_tag="ACG8XG_000888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772821.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            27526..28125
                     /locus_tag="ACG8XG_000889"
     CDS             27526..28125
                     /locus_tag="ACG8XG_000889"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0772822.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            28141..28617
                     /locus_tag="ACG8XG_000890"
     CDS             28141..28617
                     /locus_tag="ACG8XG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772823.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            28634..29536
                     /locus_tag="ACG8XG_000891"
     CDS             28634..29536
                     /locus_tag="ACG8XG_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772824.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29609..29800
                     /locus_tag="ACG8XG_000892"
     CDS             29609..29800
                     /locus_tag="ACG8XG_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772825.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            29964..30551
                     /locus_tag="ACG8XG_000893"
     CDS             29964..30551
                     /locus_tag="ACG8XG_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772826.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            30551..31450
                     /locus_tag="ACG8XG_000894"
     CDS             30551..31450
                     /locus_tag="ACG8XG_000894"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0772827.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31450..32364
                     /locus_tag="ACG8XG_000895"
     CDS             31450..32364
                     /locus_tag="ACG8XG_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772828.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            32416..32631
                     /locus_tag="ACG8XG_000896"
     CDS             32416..32631
                     /locus_tag="ACG8XG_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772829.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12444 a         4451 c         6662 g         9352 t
ORIGIN      
        1 atctttaggg cgtaagccct atttttttaa aattaagtga taacaaataa atgatttctg
       61 aaacaaacgt tcttactcaa ctactcgaaa cacacaagct aattaactag attataaagg
      121 agaatgcgta atgaaagaaa tcagtgcaaa tgaagctcat attccgcaaa agaatcaggt
      181 tggtatatat aaggtgcttc atggtgaagg aatttataaa atacagttac ttaccggtta
      241 caataatggt gcaaatggga agtggttaaa ggcaacaagt attcgtgaac ttagaagtga
      301 agtagaaaaa attaggcttg gtctcacaag tgaagaaata gctaaactga agggaaataa
      361 ttcttttgta attgaaaaag aaaaggaaaa acatctactt tatcgtgagc tgaaaattga
      421 agaatactat gaaattgatt tacctgaaaa attaagaatt attgatcaac gttcagaaga
      481 aaaatggaaa actgaaatat atccaagctt aaatgactac aagacatcag gaaagcccat
      541 gtataactac acaggctttt taacctacac acaatacaaa tatgcctttc gcaaattaaa
      601 aggcattaaa caagctgaaa ttgccctgta tgagatgaaa aaagtggata gattgaaatt
      661 gattcaagat ttagtgcaaa atacatggaa tcaagcgatt gtcgagctag aaaaagaaaa
      721 tgggaaaatt ttaactaata aatatctgat cgtaaatgaa attgaagaat taattggttt
      781 attttcatgt gaaaaaggtg aagtcttctt gaagtcatca ccaacctatt caattttgga
      841 cagtccgtat cagtttatac aggcaaggag atgagttaga tgtctagctt gaaagatgac
      901 tatatccaag caaaacaaga gtatgaaaaa atacagatgt tgaaatctga gaaagtcgaa
      961 ttatttttaa cacaaaaatt agcagaggat catattgcta acttagatgc taaaacttta
     1021 gaggaatatg atcggcagtt taatcagata catcacaata tcattttgct tgttgagcag
     1081 gcaaagcgaa ggtttgaaaa agcggaatat gatctattag atcaaggaat tgtatgcttg
     1141 gataagggat tgcgaagata ttatggtgca gagttaaaaa agcaaaatgt agatagaaat
     1201 gcactaaaac atgaaatctt agaattgata atgcaacgaa tttgaaggga tagaaagcaa
     1261 atgtcacaaa acattagtgc tgtaaaacaa ttaatagaag aagatttaga gaccacagtt
     1321 cagagattag cggaacgaat ccattttgca aattatcaaa ccatctctaa ctggtcaagc
     1381 aggaacagaa atccggaaga aattccaatg aaattttttt atcgaatcgg aaaagaatat
     1441 ggtgttgatc cgataaaaat tttcgagaag accatgattt attactatca tggaacacct
     1501 aacgaagtgt tagaacatct tttggaagag gcaaaagaaa aatcatcaat attagagcta
     1561 tcgcaaaatg acaaagttat tcaagtgaaa tgcgttgatc caacaggaaa gattattgtc
     1621 ggttttgata ttgaaagaaa atccaaaata gattttatta cacaattaaa aaagataaat
     1681 aatttagata ttctaacgct tagaccagat catgaacagt tgtttatgag ttttaaaaaa
     1741 caattgaaag aaatacaaaa aagatataac aaggtgtttc ctaatgcgtg aaaagctcaa
     1801 agaaatcaga gttttttgga taattaacaa agaatgtatc atcttatata ctcttggatt
     1861 tcttagcatg atcttgtttt ggtggttata tttaaaacag taattggaga gggtcaagag
     1921 gatttttatc aagataagga tgtggaaact gcatgtggaa taatagatta aaaacaggat
     1981 tgttattaat agttatttcc tgcgctatga tgattggtat gaggatacaa agggaacaat
     2041 cctattttga agtttctgct aacaacgtaa tagaaaaatg ctattacggt caacattatt
     2101 ggagtgagga agtcagagaa aatatagatc gtgaatatgt tcaaagaatt gtttgggatg
     2161 catattctat caaagattat cctaaaagtt taacgagtag attattttac agtgaaaaag
     2221 ataatcaaaa actctcagat ttgatgatga aaaaagtatg actattatta cgaaagagaa
     2281 acagagtgct atattcgagg ggaaaaagcc tacaacaaaa gtgactattg gggtgacaag
     2341 gcgaagcgat taactgcaaa ttttagagtg tatgaatcag agtttataaa cttgaccttt
     2401 cttaatagtg tttttattga ttatgtaatt actacgaaac gcattggaag ttattcaagt
     2461 acctacgcat ccttagttcc aatgttaaac tcgatgagcc gtttcttaaa agaaagagaa
     2521 aagaaagagc tacagtatat caaagaaatc atgccggatt ttgaaatgca cgaatggcaa
     2581 attgctttat cagattggaa attggcgaat aaggttcgta gaataacacc ttttcaagcc
     2641 aagcgctttt tgagagctat gaaatttgtg caataaaaaa agccaccaga caagttgatg
     2701 gagactaaca caattaccaa cagttgtaat tgtgttagct tacaaattaa ttatggtgtt
     2761 tagtcaatcc caaattgtga aatggcacca ataattggaa aggaaaaatg aaatgtctga
     2821 agatttacga gagctgaagt tacgaaagaa gcagtatgat cagtattttt ccggtttgtt
     2881 gaataaacat attgaagaag gacttgatca tgattttgga aaagatgaat attgtttata
     2941 ttgcgaacgt tacaatgaaa aaatgtctga atatctacag ctgaaaaaag agatacagaa
     3001 attagagaaa aaagtacatg cttggaagta taaagattcc atgtctggtt atgttgaatc
     3061 tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta aagatggtaa atggggaaga
     3121 gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag
     3181 gaaaaaggtt gaaacaataa atgagataga cgacagccaa tacaatagca tctatattga
     3241 aacaagtcat ggtggatata agaaattagc aactttcctt agaagttcta aagttgggat
     3301 aatttctaaa aaagacataa gtgctgttac cgatagtttt tgtactttat ctgttcctaa
     3361 agcttatttc caatacgata gtaaaagatt tgaacgctta gagaaattct gtagaccgta
     3421 tactgtattg aaaactaaaa aatttaataa aactaaaaga gtttctatac agttaccgac
     3481 tggtcaaatc tcattaggtg aaaaagacta tctgataaaa gtaggaaaag agttaattat
     3541 aaaagtaccg gaacacttat ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa
     3601 agaagaaaaa tagtttggaa gacatagaat ggtataaggg gaaattgaaa tggcagtaaa
     3661 taaaatattg caggtactaa tcatagacga atttggcgga ggagaatggt ttgatgtgca
     3721 gtccatagac tttgaagaag aagtcgtagt tatagaaaat atttacggcg aagtatattc
     3781 atattcgctt gaaggtgaga atattgagga tttacggttg attggtgagt agaaaaaaac
     3841 agaaatatca aaatggagat aaaaattaat agcatgtaaa gatagtcatg cttgggttaa
     3901 aaatgttaaa attcaaaaaa atgagtcaga agggataaaa gtatgaaaag agaggaatac
     3961 aaacaaagat taaacgaact gcttgaagag gacgaaacat taactcatgg ttcgcctgat
     4021 gaaattctat acatgattga taatatggtg atctttggtg gatatgaact tggtaatagg
     4081 tcagttgatc ataatatttt agaatttgat gatgtatcat gggaagaaat tcttgattgg
     4141 ggaattttgg cggtacctga aactaaaacc tatattagtg acactatggt tccttttttt
     4201 gaggaattgg attataaaag acttcccaaa aatgaaaatc atatactggg aggaaactaa
     4261 caatgttaag tgacttattt attttactga tctatctagg tgtaagtgaa attattgcgt
     4321 attttttgtt tgtcattgct aattatggta tttccagtgc aatcgttcaa atgtttagat
     4381 taccagtaag tgaactaaaa aaatgatgag attatcagca tatcttttaa tgtttttgaa
     4441 tgtggtcacg ttaatctttg tagtaatcgg tgtgactttc gatttaatgg aatagtggga
     4501 aatctataat ggaaaaaaga gacacgaatg aacatatttt ttgaatcaat attgttgatg
     4561 gtttttgcga tcagcgctta ccagaaatct aaaaaagagt aatgattgtt taatcatcac
     4621 tctttttttg tagggaaatt gaaatatgtg gagcttgtat tggtttagtt aggattgctt
     4681 ggctataagg agctagttct ttatcaaaac catcaaagta ggttttcagt agcgatttaa
     4741 ctgatcgctc atcttgtttg atttggataa tttcagcatc caaatcggtt gtgagatcag
     4801 gagcttttga tgcgattacc tgattttttt tatcttccaa ctctcttagg ctgtttaaca
     4861 agttgtaaaa agaatcattt tgattgttca ttccaacgtg agttgcaatt ttaacagaag
     4921 tttcgtccgt attgtcatca aaagaactca ttggaatatc aagtagtaac gaggatgttt
     4981 cttttactga ctttttcttt acagcatttt ttgtttttcc atcttttttg ctctttactc
     5041 gagatgtttt tggagctagt ttaggtaaat cacttgtttt atcagtcatt tattagcttt
     5101 tcctttctat taatttatgt tattatatta tcataataac ataaaaaagt acactttttg
     5161 ggggcgtagt tattgaaaaa atctaagaga aagattgcca cattgcttat gttaatggtg
     5221 caatttacag ggattgcaat gagtgtaatc acaataaatc cgcaagtgat acatgctgat
     5281 gggatagcta cttatgatgc acaatttaac tgtactactg aaggtagtgg gtcaggaaca
     5341 gatagcgatg gaaacgatac tggaagtggc gctgtaacag gtgatgtagc caaaaatatt
     5401 gaagatattt acaaatacat gaatggagaa tatggcttat caggtgctag tattgcaggt
     5461 attttaggta actggaaaca agaatcaggt attgatcctc gagcagtaga agggtattac
     5521 ggagatggac agagagaagt tgacttcatc aaacaattta ctgatggtct gatgggtatt
     5581 ggctatggtc aatggacagc tgggcgacat acagcattag ttgattggtc taagaaaaat
     5641 aatggcggtt cttggtggac tacagaagcc caattggact ttatgttcaa aggtgatggg
     5701 ggattcgtag gaatattaaa agattatgct ttaaatgctt ctgatgatgt cgtagaaaat
     5761 gcagtaactt ttcataaaaa ctgggaaatt tcagctgatt ccgaatcagc tgtaatagct
     5821 gtacgtggtg aaaatgcaaa agaaatctgg aagtacatga aaagcaaggg tatggacggt
     5881 gcaaaagatg aaagtaagat caataaaata tccggaggtt caagtaccgg agatggatcg
     5941 aataattcat caagttcagc atcaggaaca gtagaagatg cctgtgcaac tacggatgac
     6001 gctaattctg gttctggatc aattgggaaa gaaggaaaaa ttaacggtaa aaaaggtcaa
     6061 gtaatagcga atatgactta tgaagaagct ttgaaaaaat atgggaaagc gatcacgcta
     6121 ccaaaattca aagaaccaga ttggtcaact tcacctttta acggaatata tcaaggtcag
     6181 tgtacagagt tgacgtgggc ttatatgagc caattatacg atgggaatca accgactact
     6241 ggaaatggtg gttatgtatg ggaaagctac aaatcggcag gagcaaaaat aacgaacaaa
     6301 ccaatcgtag gttatgggtt tagtgcttcg gatggatggt tatgggctgt tggagatgca
     6361 gggcatactg gtgtagttat agcggtgttt gaagatggat catttctatg tgctaacttt
     6421 aatgttccac caacccaagc tccaacaagg ggagtttcgg taacgttgat tgatggagtg
     6481 gacggatcag ataatgtgca tttctttagc ggtgtaggaa aagctaaatt tgaaatcgaa
     6541 aaataaattg gaatgaagga cgatatagat gaatagtaaa gtgaaagata caattatcat
     6601 ttctttgctt tctggtatgt tattagcact gcttgttgga atcctattct tcaataagca
     6661 gaatcaggac atagaagctg aaaaacacag tgtgcatatt gaagataagc tgacattctt
     6721 taaagaggtt aaaccaaatg aggtggatgg attacggaaa gatttagaaa atgatattca
     6781 agaaatgttg aatggatcag ttcaaaataa gtttgcagat caatcaggtg aagaattttt
     6841 gagaaaacta tttattgtaa caggtaaacc accaattact gataagagta gtgagaaaga
     6901 aaaaatcgag tactataagc cttttacttt taagctaaca aactttggaa ttaagacatc
     6961 tagtagtggg ttgtatgaag cattgaccga ttttgaagtt caatacaatg gaaaaaaagt
     7021 gtatgatacc acattaacca tcacactaaa taatgataag aaagtgactg ggggtgcttt
     7081 ttatgcaaaa tcatgaaatc actactgcta aaaaggcgat cggaatactt tccataattg
     7141 tgatcctttg ttgtgtggta ctatttcaaa agaaaaatgc ttatgataat aatgtcaaag
     7201 aattggaaga ccttaaaagt aaacaaatcg aagtagtcaa gaataaccag aaagtgaaag
     7261 ctatgcagga agctcaatac aaagaattgg gaaatgctga aattaaggaa caaagtagga
     7321 aattctttaa tttattttac aactggaatt catgggaaaa atatacgaag aatatgaagg
     7381 aaatacaaaa agaatttcct cagatagtaa agtctagtcc tgttgatatt tctggcacga
     7441 tggtaggcac tggtaatagt ccaatatcta gctatactgc tgattactat acaacaaatc
     7501 aaaaaggtaa aatggttgaa ttgattacac agacaagaaa tactccaaca acagcaacaa
     7561 caacaatttg gcgtggagta acaagcatag gagaaaatca attgttttat ttggaggatt
     7621 tagatagcta tgtaagagca gagtaaaata aaaaaagagc taacggtacc aacgttagct
     7681 ctttgtgctg acgatgataa caaaacctac cgaaaaagga ttgtttcatc acaaacacaa
     7741 acagaaaaga attgataaaa aacccgaacc tcagaatttt tattaatcaa ttcatttaga
     7801 ttataataaa ttgagctgaa cattacaata cgtatatgtt gtaaaacgta aaaaaagtca
     7861 aaagtaaata ctttcttaaa aagtcaaata aaactattaa agacaatata tatggtttat
     7921 ttgacataag aaaatggtct agatgataat tgtaatatag aattgtagac ttaaataaag
     7981 cataaaatac cttttatgat aaggtatagc tcttaatttt aagcagtttt agatataaga
     8041 ttgaaagtaa agtgggggag aataataatg gcagatcata aattagaaaa gttattagaa
     8101 gaaacgtgga atccaaaaga atttagtgaa ttttttatgg agaattttga aactgactta
     8161 gcagtgattg ttaaagatgc tcttagagag caagggtatc ctgaaactgc aaactatata
     8221 aacattaatt ttactttgta tactgaaaat aaaggaactt gggatttttg ggctacttta
     8281 gcaaataagg agttaagtga taaatcagac acaggaattc gtaacttttt tgaatcaaac
     8341 agagatgatt atatgtatgc taacaatcaa aataaattga actttagagt agagtttgat
     8401 gaaacacctg aagaatttat agaaagacaa ccgcctaaag aaaatgtagc aaaggtattg
     8461 gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta gcgaactagg cggacaatat
     8521 gaaccgctag tagaagcggt tagagaagag ctaagactga ataagtttcc tgatgttcaa
     8581 aatattgatg tttctcaaat tgaaataaac gtcaagatca cgaataaact tgattatggt
     8641 agttgggcag atatagcatt agaaaaatac atctactcaa cgttaaaaga gtttatcgaa
     8701 aatagaatgg atatcatgta tctacagcat ccacagtatt taaactttgg tgttgaaata
     8761 gctacacctc ttgaagaatg gaaaatggag caagggcttg actagccttt gctccatttt
     8821 tttattttac aaatgaatac agttcgtcgt ttttaccaag aatgattgtt tgcccaactt
     8881 tgagattgtc aggtgatcct tcagcggttt gtaaggagaa cgttccattg aacttatcgg
     8941 ataccgttat aggtagcttt aattctgatc cttcctcatc tttgagaatt gctgtttttc
     9001 gttcatcaaa ataaacagtc tttattaaat aatttttcgt agtgatctca gcagatttgt
     9061 ctttttttgt agtagtacca gtttctttag aattagaggt attttctacg actattccat
     9121 ctttggcatt tgagcttgtg tttatttgat tgaatatttt cagcgacaag ataacgctaa
     9181 gaatagtcaa tatagaaaca agtgtaatta atactgataa tttccttttt ttgttcattt
     9241 tttcttaaca tccttttttg ttttgattag ttagatattc cttatctaac aagccttttt
     9301 aggctattaa atgaagataa accccaaaaa aatattgcaa ttatgacagt gcaatggtac
     9361 tattattata caataaattt tgcgaaaggg gtaggtctaa atcgcaaaca aaaagaaatt
     9421 gaacctagac aaatacaact tgtataccgc aagggaagca agtttgattt tgggtaaagg
     9481 tagaaattat gttacaaaca taatcaacta ttatccaaat cgtttgccta aaggttcgtt
     9541 caagaaagtg ggtgacaaaa cctatattat aacggaagaa ggaatagaag cattgcgatc
     9601 acaaacagaa aataaaaaca aaaagaagga atgaatatga agagaaattc gcttggtatg
     9661 gttttattgg catcagctgt acttgcgggg ttatcagtac cagtaacctc acatgctgat
     9721 gaatggactg caaacacacc ggatcaaatc caaattacaa caggacaatc aagttatgcg
     9781 ttcaaaaaag gagatactat ttggtcaact gtagagaagc ttaaaagctt aggttacaag
     9841 actactgttg ctgagtttgc cgaattgaac aatgttgatt tggcaaaagg agaacaacgg
     9901 actatcccga ttggacgaac tttcaacatc agtaaagata cattaacagt taaggaagca
     9961 gatggcgaag tagttagtca aactgttcaa aaagataaac ctgatcctat tgaaaatacg
    10021 gagcaagaga acaaaaaatc cgagactaca aaagaatcgg aaccaacagc aaacacttct
    10081 gataagacag aaacaagtgc ttcaagtgtt gatggagcaa gttcatctaa tacaaataat
    10141 tctacaagtg agaaagaagc atcacaggga acaagtgaaa gtagcactac ggaagctgat
    10201 actggaacat caacaaataa ctcacaatca acatcaaatg atgcaaatga atcaaccagt
    10261 acatcttcta gtgatcaatc acaaacaggt tcgagtgatg aaactgacag ttctacttca
    10321 ccaagtaaac ccgaacaacc aactaatgtt gtgctaggcg ttccatttat tagtcaaggt
    10381 aacacaatgc tttgcgaagg aacatctttg ctagaagcgt tacattataa aggtgttgca
    10441 acaaatcaag atttaatgac ttttgttaaa tcaatgccac tatctcctga caataatcca
    10501 aataatggtt tttctggtga atggagacac aatgtggatg gaacttatca aggtatgaat
    10561 ccggctcctg tagtttcatg gggtgttgct aatggcggaa atgttgtaga tatttctggt
    10621 tcatcggtac aaggattaaa agatgagatt gtaaatggaa atcctgtagt agcgtgggta
    10681 acttatgcct ttgaacctgc tcaacataaa ccaatgtttt ggggcgatgc tatttggaat
    10741 cgacatgtag tagccgttga tggatttaaa gacggttttt accatgttgt cgatcctgtg
    10801 tttggggcaa gttggattaa tgcaggatca tttgaaacag catataacgt atctcaatta
    10861 gcggtttcgg tacgttaata aaagatatcg tttatatact aagtagttat atagtcttta
    10921 tttgtccttg cttaactact tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg
    10981 tgatgctatg aatactagag cgttgaaaca gcttgctttc aacgaaaaaa atgattaata
    11041 ggtgtggtga ttttaactta ggaatgggcg cgaaggtcat taccacaccg acatatcgtg
    11101 gctcattttc ttcgcctaac tggtataaag aaggagtaac gagagatgaa gcatgaaaaa
    11161 acgataattt taaaatcaaa aataatttat tgttttgcga ctttagtaat gatgttatct
    11221 acgcttataa tgcctttttc agcaataaga gctgaagcag atgtcacaaa accgacaagt
    11281 gcaaaattta tagaaactgg cgtaaaaaca gacggttata ttagggttaa tatgccgaat
    11341 catccaaatg aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa tattggatat
    11401 tgtatggatt cagaattgcc atccccaaca ggaagtggag ccggaagctt gaagtacaag
    11461 ggagctggca gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc aaaaacagca
    11521 aaggaactgg gtgcaggaaa tgatacagaa gcatggtatg caacacaatt agtttcatgg
    11581 gttttagccg gaaactttaa agtttcacag attgtatggt cacatccaaa ccataccgcc
    11641 gcagaaacag ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc taagaatgga
    11701 aaagatacgc ccaacactga gttttctatc acagcttcta agacagctga cgaaggaaaa
    11761 tatcacactt tcacatataa aacagcatca aataagactg gtaatgcgaa gctcactttt
    11821 acatcagcaa aaccagctgg tatgaagatt tatgatgcag atggtaaaga aattactaac
    11881 aacactgtga aattaaattc aagttttact attaaagtac ctgtgactac accaagtggt
    11941 actttgtcat ttaaaggtac tgctaatgtt agcacaacca atccttttac ttttgatgga
    12001 cgaggtgtgt atcaagatgc cgtagttatg atcactactt cagaaacaaa ggactctaaa
    12061 tctttgagtg ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa
    12121 gatggaaatg ctttaaaagg agcggagttt actctaactg acaataacaa tgctaaatct
    12181 gttaagacaa ccggagatga tgggttagct acgtttgatt tgcaacaagg cttaacatac
    12241 agcttagaag aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt
    12301 actgttaata ccgatggaca agaatttagt tacactgcta cgaacacaat taaaaaaggt
    12361 aaagtaaaag ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc tgaatttacc
    12421 ttgacggatg attcatccca aacacaagca gaaaaaacag atgaaaacgg acttgcaagc
    12481 ttcgatattg tagcaaacaa gacctacagc cttgaagaga ccggagtacc aactggttat
    12541 acaggcgtat tcaagcaaga aggaattacc gttaaagatg atggacaaac ttttgaatat
    12601 acagctacaa acactttgaa aaaaggttta gtaaaagttc ataaagtcga tcaagatgga
    12661 aaacctttgg caaatgcaga gttcacttta accgatgaaa aaggtgcaac acaagtagaa
    12721 aaaacagatg aaaatggact tgcaagcttc aatattgtag caaacaagac gtatagccta
    12781 gaagaaaccg gagtaccagt aggttacaca ggtgcattta agcaagaggg tatcaccgtt
    12841 aaagacgatg ggcaaacttt tgaatataca gctacaaata atttgaaaaa aggtgtagta
    12901 aaagttcata aagtcgatca agatggaaaa cctttagcag atgcagaatt caccttaacg
    12961 gatgaaaaag gtgtaaaaga agttaaaaag acggatgcaa ctggtctggc tacgttcaac
    13021 attattgcga acaaaactta caacttagaa gaaacaaaag taccaacagg ttacacaggc
    13081 gcattcaaac aagaaggaat taccgtcaaa gatgatggac aagtctttga atatacagct
    13141 aaaaacactt tgaaaaaagg tgtgatcaaa gttcataaag tcgatgatca agggaaagca
    13201 ttagcaaacg cagaattcac tttgacggac gacaaaggtg taaaagttgt acaaaaaacg
    13261 gacagaaatg gcttggcaac atttaatatt gttgccaaca aaacgtacaa aatcgaagaa
    13321 acacaaaacc ctaatggtta tacagggaaa tggtctaaag aaggcatcac cgttgctgac
    13381 gatggacaaa cctttgaata tacagtaatt aatcaccttg tacctgaaac agtaagaaca
    13441 ggcggaatcg gtacatcagc cttccttctt gtatttctaa ttgcaggatt gagtatggtg
    13501 ctactgtatt atcgagaaca gcacaaagca aaataaaaaa gtgttaatgg aaaagaagta
    13561 aatatgaaaa aagaaggctg acgtatgaaa tcaaaaaaaa ttttttcaaa tttaatgcta
    13621 tttggtactt tgctaggagt gggattgggc gcaagctcaa tctcttctag ccttggacta
    13681 ggggggcaag tagcatatgc acaacaaaac tataattatc aaggaactgg aacgattcat
    13741 atccataaag taacaaatac taatggggat gggaaccctg tctcagatat tgataacaaa
    13801 gggaaagaaa tagctttacc aaatggtgta aaacctcttg aaggcgttga gttctcttat
    13861 gtgaaactta ctaaagatga agctaaatcc ttgggatgga cggatagtga tactacaaaa
    13921 ggtcaacata ttgatgatgc taaagcacga ttattactaa acaaaaaata cagtgaagta
    13981 agtagaatga agactgattc acaagggaat atagacatac ctatacaagt aaatacagac
    14041 gatttaacag ataacttgta tttgattgtt gaaacaggaa cagtcaaagg tgtagttgaa
    14101 gaagcgtatc ctatgattgt taacgtacct gaactaaacg gatcagatga tcaatacttt
    14161 aattatgatg tacatgtcta tcctaaaaac tatttccaat taaactcagc agagcttgat
    14221 aaaaaagacg aagatggaaa ggctcttgcc ggaactgttt ttgaattgcg aaaagatgat
    14281 ggatctgatc aggttgttgg aacttataaa acagacagca caggtaaatt aactgtggat
    14341 ggtttggaat taggtgacta ttatttccta gaaaccgatt tgggaacaga tgctaataga
    14401 acaacctatt tactagatgg taaaaaattc aaatttagcg ttaaacgtgg ggagactgct
    14461 aaagtagttc ttgatgttac caactacaaa cgacctgtaa ttactaaggt aattgataaa
    14521 gaaaccgttg atactggcga agatgttcaa tatacattga aaccatcaat tcctggtaat
    14581 attggtaatt acgaaaaata tgttgtcaaa gatgttctag gtgcagagtt aacttatgat
    14641 tcatcagaag tgatcgaagg aatgacttta gatactgatt acagtatcga acgtgacgga
    14701 caaacgacaa actatgtctt tactgaatca ggtatggata agcttcagaa acaatttgat
    14761 gcaggggatc gcttctttac aattttgatg aacgctcata ctaatagtaa agctaaaatg
    14821 gatacaccac ttaagaatga tgtttcaacg atctacaaca atggatatga acccgaagac
    14881 gaaacaccag ttgatcacgc ttatacaatc attggtgatc accaattcat taaaacagac
    14941 ggagatacaa gcaaagcatt gagtggagca aaatttgtag tgaagaacaa aattgatccg
    15001 aataaagtta cttacctaaa acaagatcca acaacgaaag ctgttacttg gattgcagat
    15061 aaatctaaag ctactgaatt cgtttccgga tcagacggta agttccaaat tgaaggtttg
    15121 caatatacct atgatgtaga ttttgataaa caaggtaatc caacaatcac agataaaaaa
    15181 atcaatgatt atgctcttga agaagttgtt gctccttcag gatatacacc aactagaaac
    15241 gatgttccat ttgtggtatc gaatgatgat gcttctagcg aagtaacaga catcaagaac
    15301 cttaaaacac ctgaaacagt tcgtacaggt ggaattggaa cagtagtatt tctagtggtt
    15361 gcaggtgtgg caggaacagg gctatatgtt gtctacaaac gagaacaaca caaagcttaa
    15421 tgaaataaaa ttccctagag tgtttttggc tctagggttt ctttttaaaa agtgtgtgaa
    15481 aagtaggtat gagcatgaaa aagaagaata tcaagttgat catcttaaaa attgggatgt
    15541 tgctatgttt aggagtaggt ctatatcctg taattactca ggcttatacc tactaccaaa
    15601 ctcaacaaac actaaactcc tataaggaaa aatatgatga agcttctgaa aaggagaatg
    15661 aagaagtctt caagagatat tcagaagtta ataggaaacg aaaagacaca gaacaaacct
    15721 cagtcacgat tcctgaagat aataacaata ttccgtcaaa tggaaatctt cagaaggata
    15781 aattaggtga attagtgggt gaaattctta taccaaagat taatgtggat ttgccaattt
    15841 atgatggtgt aaacgatttc caactatcag caggagcagg aatattaacc ggaactgatt
    15901 atccaactgg tgggattgga aaccattctg ttatatcagc tcattcaggc attcctaata
    15961 aaaaatattt tacagattta gagttgctca aacttggtga taaatttttt attgatattt
    16021 cagggaaaaa acttgcttat caagtagatc ggataaaaac aattctacca accgaatttg
    16081 aggatttgct tcctgtggca ggaaaagatt atgtaacact tatgacatgt acacctgttg
    16141 gagataattc tcataggtta ctcgttagag gggttagcat accttaccaa aaaagtattg
    16201 ttgagaaaga aaagaagaaa gaagatttaa aggtaaaggc gcagatgaac tggtatccta
    16261 tcgtagttgt aggggtttta ttaataatag ctgtgatagt cattattaaa aaaatagtag
    16321 caaagagacg aggaatgaaa aatgaagaat aaaatactac tttttttgac tcttatttgc
    16381 actttggttt taacaattta tcctgcaaat cagctctatc atcaattcaa aacaaaagag
    16441 aatgtggcgg aaatccaaga gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg
    16501 gcaaaaaaag tcgaacaaat aaaatcgaac agcggtaacc aaggattaga caatactcag
    16561 aaaacagata aaggagtaga caatactcag gaaacagacg gtggatcatt tgatattcgt
    16621 gaagctgaag gaaaggtcaa tccatacaca ggagatgttt tttcaaaaaa tcaaacgaaa
    16681 gattttgaac catctaccta ttcaggagcg gttggttatg tctatattcc aaaaataaat
    16741 gtgctagagc ttttgtttct aggcgcttca caggagcatt tatatagagg tgtggcacaa
    16801 atgagtgcta ctagtctacc tttatcaggt aaaggtcatc aaacagtaat agcaggtcat
    16861 cgtggcggtt atacgtcacc acaatttctt tatgttgatc aattagtagc cggagaccac
    16921 atttatatag attatcttgg tgaaaaagca gtttatacgg ttttccgaac agacgttatt
    16981 agaaattacg gtcagaattc aacactgact gatattcctg atttagatat gttaacactt
    17041 tatacttgtc atccatatcc tcagaactac gatcgtttat tagttagaag tctaagagat
    17101 gaaaaagcaa aatacatcac gccacaagta acagttggca ataccgcata cgctgatgcc
    17161 attgatagcg agtccgtttc agataagaca gaaactgtta catcttctaa tgatttattt
    17221 gaggatgttg atcgtgccaa caaggaatta gatggaaata atgatcttaa tattccggaa
    17281 caaggtagcg tagagatgca agacagaacg tttgctgaaa aggcaagttc aatatttaca
    17341 ggaattacca ataaacatca gaaaatgctt ttagtcattt ggactgtaat aggagcatta
    17401 gcaagtctaa aggttcttat tcttttaatc aaagagtttc gaaaagcata atgaagatta
    17461 taaaaattga tagagtacag taaaaaggga ttgatactat tattgatctc ttttttattt
    17521 tgaatagagc tagattcact tttaaataaa atcaaaactg atcttaattc taaagaaaaa
    17581 gtcaagaaaa aaatacaaca aattttttta tcagaatatt tatcagaata ttattagaaa
    17641 agataaactc aaaagcttaa tttaataggg ttattggttg tatttagaaa taatgtatgg
    17701 aaaagctata aatcaataga taagatgtag aaaatcttat ttgtgaatga aaaacaagtc
    17761 atcgtaaaat attaatagga ggtgtagtaa tggtgggaat atcaaaaaca gctttagact
    17821 cattagctaa actatttagt agcaggtttg atggtgcaga atttgttgaa tatctaagcc
    17881 aaaacgaaag tggaaaaact ctgatcgaaa caatgaaaaa agactatttg aatgaaaatg
    17941 actttccgac atggacgaat attgaagatg ctacatggga tgtagtatgt acgatatatt
    18001 ctccttgggt aacgattgct gattttgttt tagataaaat tggaggggat acataccctg
    18061 atgccaaatc tcttgctaca agtgacttta aaacgttcta tggttatcta ataggtctaa
    18121 taggcaatgg aatagatttg aaagttacgc caaaacaaac acctgaagaa attgaaaaag
    18181 aaaaaggaga ggttcaaatg gcttatgcaa atgacagaga aaaagtaaaa ttattggaag
    18241 gtaaattatc tgaagaagaa cttggaaaaa tggtgcaaga aatcaatgaa gacagcaact
    18301 attttcctga attgaaggta tatcaaaatg acgaagattt ctttaatgaa atgtttagta
    18361 atagtccatg ggaagcagtg agtaaaacaa atttaggaga atggaacttt caagatgatc
    18421 tagttgcatt tagcaatgat gggtatttac gatcatattc agaaagtgag tatagagctg
    18481 aattaaaaac gtatgaaacg gatattctga tgagctattt aaatctatta gagagtggtc
    18541 agggagagtt aagagataaa gttgaagaac tattaaaaga agagctatag ggattatttc
    18601 aacataaaca caagcagaaa actactaaac aaaacgtacc atgcaaaaag tcggttaaac
    18661 cggctttttg ctctattatg agaggagatc aaaccatgag tgcaacaacc gatatggctt
    18721 tagaagcagt aataaaagat aatacaggaa atgtcgaaaa cgtagaagac tttagcgata
    18781 ttgcaatcaa tctttcagca tggatttcag ctgtaggaga agaaaatatc gaatttgaga
    18841 tcaagaatat ggatgactta aaagcaaaat ttccttttat cgcatcgaaa ctaagtcaag
    18901 ttgccgagga taaatcgtgg aatgaattag ttagtttttc cgaaaaaatt tatgatatta
    18961 cgcaagaaga tgagtgtgtt tcatgggaaa tgaataatca atacaagaaa attgacgctt
    19021 tcgtcaaatc acgtatagaa ggaacagctt ttgagtttga aggtatagat accgcagata
    19081 attggttaaa taaagaatcc tttattaaaa gtatagcgga tgatcttgaa aaacaatggt
    19141 ctaaggaaga ctatgcagag tacctaaaaa cagaaagaga tacagaggtg aaagaaaaac
    19201 tcactgaggt atgtttggat gaacatattg atcccaatat cgtaaaacaa gaagactatg
    19261 aagtggttat tgataaagtt cctactgatt ttgaggaagt tgtaaagtat ctagttgaag
    19321 aagcattatc gcaatactct tcagatgagg aagtggacga agaaactgtt cgtaccagag
    19381 tttggcaact ggtttttgat gacaaagatt ataaatattt gagtgatcaa aactcggtta
    19441 attggagtgt acgattatta attgatcaaa atgatattga ataaaataga gctgaaaagc
    19501 tctatttttt ttgttaaaaa aactagctat tttattaaaa ataatttggt tcaaatttta
    19561 tttttttaac ttgataattt cttgccatcc ttttttaaat tggctatata tcaaggttta
    19621 tagtgggaat aaataaaaat acatttgaca aaatttacaa tatagtttat tatatattta
    19681 tcttattatg ataacacaat aaaaggaatg gtggtatgaa aaagaagaag tttagtcgat
    19741 cgttgttagg aattttggtt ttagttgcgg ttggtcttag tggcaagggt tactttcaga
    19801 ataacagcac tcaggaacat gattttcaga tcaaaagtag tgttcagata ggtaataaag
    19861 cagacagtga atcaaaagaa tttttaggga tacgttatga tccaaacaaa tatccaaata
    19921 atatcgtaaa cattaatgat gggaaatata cactgacaaa agaagaccat gaagcattat
    19981 cagaaaaagg cagtcaaacc ttttgggtaa attatgcaga tttagatcaa cttggaagag
    20041 caggagatgt tgaagcgttg atcacctatg atgctgtaaa ttctcactca tctaaaaatg
    20101 tggaacgtcc atctttcaaa agttctgtcc atattgcagg agaatacaag gatggagtat
    20161 ttgatcaaga gagagatact tgggtcggca aagattcaaa caatgaaatc actcagctag
    20221 aaggctataa aggttatctc tataacaaaa gtcattcatt agcatggtcg ctaggaggag
    20281 atatggaggt acacaattta acacttggaa cgagagcgca aaatgttggg aaaaatgatc
    20341 agaatggagg gatggcttac tcagaaacga ttgtacgtga tgcaatctat aaaaataaga
    20401 atgaaaaagt attgtataaa gttgatccaa tttataaagg gaatgaatta gttcctcgtg
    20461 gttctcatgt cgaagctttc tcagttaacg ataatggaaa aacggtaaac cttaatgttt
    20521 atgtatttaa caatcaaaaa ggaatcaaca tcaactatca aaatggtact tgggtaagag
    20581 attagaaatt gagaaggtga agaattggaa acaataagca tcaatggatt agataaatca
    20641 gttgcaaatc gaataaaaaa aggaaatggc tactatcttc tgaagaaggg taaatattca
    20701 gttgctagag agttagaaac aatgagtatc gtgtatgtag ctaagaatca ttttgacgta
    20761 gaacaggcta tttttgatcc agattctgaa atagggaaat gtgttgaatt atcagagaaa
    20821 actgttagta caaaaggggc gatggtcatt gagagagaaa tgttagacga aatcagagta
    20881 atcattccta aagatacgca gatgtgtgag gacagcataa gtgacgatga accaaaatca
    20941 gaaagaaaat aaggagatat tcagatgagc gaaaaatcca ttgaattaaa ggaatataag
    21001 cgaagcgatg gagaaaaata ttatcctgta gccagttttt ctaaatcagt atatctggtt
    21061 ggacgtgcat tggtagttgg tgcaattggc atatatattt tgcattgggt aacaaaggtt
    21121 gtacaactgc ttgtagatac cttagtaagt attcttgtct cgttaaatga tgttaacgga
    21181 acagatgatt taggattaaa agagatgttt aaagagttac tcaatgcgtt ttttcccaat
    21241 tatttaagtt attcatataa tgtacaaagc ttttttgcaa agctacactt ggtagacatc
    21301 tctattttag tattagaagc aggaacggta ttctttgttg gatatttggt tactttgatg
    21361 ttcaggcatc actatggaga acaaagacca ttttttgatg atatggaaag tagaaggttg
    21421 aaaagagaag ctctagaagc tatggatgtg aagtatgatc caaacaagat tataggagat
    21481 gaaacgaaca acaaaaaaag aaaacgaaac aaaacgcaat tagaatccga tgtgctatca
    21541 acgcttaaaa atatcaaaat ttctgttcat acaagacgtg atctaagtgt caatgaagat
    21601 attcgagaat atatcgtaaa aatcccgcaa ccaaaaggga agaaagttag agaaacagtt
    21661 ttacgacaaa ttcaagattt agggtcgatc ctgacaagag caacaagcgg acgagccaaa
    21721 tttggtgaaa aagaagatga agtttcaggc gattatttca ttttccgagc atcagtaaat
    21781 attgaaagag aacgtaagga aagagtaagt accaaaaaca aagaaacgga aagagcaaca
    21841 tcttcaatcg cagtttcaaa ttcacaattt tcttttccaa tcaatctctt tgccgataat
    21901 tccaaaaata tccaaaacca aaccgaaaaa gctagaaaat atgccgaaac tcaaaagcaa
    21961 tctttagaac tttatctatc ttctgctgac gtacctgcaa gtctaacaga tatgcacgtt
    22021 gggaatacat ctattgaata tttgtttact ttatctcgta ccagaaaaat tcaactaagc
    22081 tcaatgaaag aagaaattga aacttacttg catatatcag gtgtaatcgt gcggatcaac
    22141 gcagggcgta ttcaaattac tttaccatta ggagaaaatg atcgtattcc aattgatgta
    22201 ccatcaatga tcaatagcgt gttttagaag acatcacaaa tagaaacaga aaatacaaac
    22261 taccagttca caaacagaaa agtaccgaga aaagtgagga atagaaatgg ctatgaaatt
    22321 attagagaaa gtcaaaaaga gagccgaaaa agctgaatct attttgctag ataaagaacc
    22381 taatataaag cttaatggaa aattcagtgt caatatgttg aacacaaagg ctaaagaccc
    22441 aacagatgct atttttggaa ttaagcctga tggcacaatt gctcattttt gcatcagcga
    22501 aatgccacac atgttggttg caggaacgac aggatcaggg aaatcagtat tactgaatga
    22561 aattcttgtt acagtaatgt gtcactcaac gcctgatgaa ttaaaaatag gaattgtaga
    22621 cccaaaaagg gttgaatttg gaagatacaa aaaattgccg ttcatgttgg ctgatccaat
    22681 tacagatatg gatgaagctt atgatttttt tgaatatctt gtgatcttga tgcatgaacg
    22741 ttacaaactg atggaaaaag cagggttaca aaacatcacg ctatataacg aatatgccga
    22801 aaaaaatgga ttagatagat ttccctatgt gatcttatta gttgatgaat attcacagtt
    22861 ggttggaaca cataaagaag tagaaggttt gattgttcaa ttaggacaaa tggcaagggc
    22921 agcaggtatt catgttatct tagcaacaca atctcctcga tcaacagttg tcactggtat
    22981 tatcaaagcc aattttccgt cacgtgtttg ccttatggta gcaagtgatt tagaaagtag
    23041 gattgtatta gatgaaggcg gtggagaaag tctaagtcca aagggagata tgcttattaa
    23101 gcttgttgat ggaagtatgg ttagagcgca gggagcttac atcagtaaca aagaaattga
    23161 aacaatcttc aatcacctta gaaatactat gccagaacca gaatatgttg attaccgagc
    23221 aatcgtagct gaaactcgtg gtgagggcgg tgaagacgag gattttggtg gaattggtaa
    23281 agctcctgta agaaaatctg ctaaaagaaa tttgaacaca cctttagcaa atccatcatc
    23341 taaagagcca tcaattgaaa agaagagtgc gtttgctatt aagcttgaaa aaaataaaga
    23401 agaaaaagct aatcagggag ctaagtcact agaaaaactt cagcaagaaa aattagaaaa
    23461 actagaaaaa gcattatcat catcaacttc tgaaactcaa tcttcaaagg gtatagataa
    23521 tggacaatca attacaacta aaaacaagcc taaaaatagt aattcaaatc tctcggtaaa
    23581 tgttgttatc acaactgata aatctgaaaa aaaacaagtg aacggaacta cttcacccat
    23641 catgcaaaaa atgataaagg caaaaaaaca ggtgcagaaa tgattatcat tagttccttt
    23701 ttgagatggc taacaccata taaatataca gatgaaatag ttgagaagcg agcgaagaaa
    23761 aaagttaaaa aaacgatgtt aactcctaaa gaaatgctag atcgtggaat attgggagtg
    23821 aaggttttag aagtaaacca accgtttgaa ttggataatg cgagagatag gttgcttaga
    23881 gctgaagttg atgtcgcata cttttatcat gataaaagca agcatcaaat agctatcaaa
    23941 ttcaatccaa aatcggcatg gcgataccaa gcaagagatg acctaaagcg taggaagctg
    24001 aaaccgatgc tctatcctga aggaaaagag gcatttgata gaacccatat tgttcccatt
    24061 ggatttcatg gtacagagtc agataaccgc ctattggtag ggtggaacag ctcacaaaat
    24121 agagttcaga tcaataaatt tgagaagaag gtaagaaaat ttaacgatcg gaaaataata
    24181 ctttggtttg ccaatattca gaagcaacca gatgggacag caaagtggga agcggtcata
    24241 tttgatgaag atggcaaaaa agtaatgtct gaagtatggc atgataaagc taaatttgtt
    24301 tggcagtaga ggtgaaaatt gtgttaacta acctaaaact atatagagag ggatttaacg
    24361 aaaaagtaag tatcggtgaa tctattgaat atttaggaaa gaagtacatt attattcgta
    24421 ttcttgaaat taaaaaactt tattttagta aatataataa tttagcagtt agagttctcg
    24481 tccaaaaggt tggaaatcca agtgactata gaagtgatta ccataactat aaaacagaat
    24541 catctgtaat taagcgttat aatcaatcaa aaaatgatga gagaatattc aaagtaggag
    24601 aaattattat ttctaagtat gggatagcat acgaaatagt ttctattaaa aaattttatt
    24661 atgaatttgt tgatttggtt gtagaatatt ctgttcgatt gatcgttccg tggagtaaag
    24721 aagaaattaa tgaggcatta aaacatgaac gaaaaagtac attcaaagtt ttagaaggtg
    24781 aaaaaaagtg aaaacagacc ttactaggca agctgagaag tgcttatggc atgaaacaaa
    24841 tcgcatgggt gtatttggtt gtttcgaagt cactatcggt tgggatggca gtgaaagagt
    24901 agattatata acttattcag tagataacac agttcgatgt tatgagatca aagttacttt
    24961 agctgattta aaaagcaagg cgaaacagac ttttatcggt gattataact atttagttgt
    25021 tactagtgat ttgtgggaga aaatgcaaca agacgataaa cttatgcgga aatattacta
    25081 tcaaggcgta tacgtgttca atgaaaaata tcctagcata ataagtgtta agaaagcaag
    25141 gaaacaaaaa gtatcgctag gtacaagatc aactgtatta gaaagcatgg ttcgttctat
    25201 gaatcgtgaa gttaagaaac tctataaagt aaaaccattt tgggagatag cagaggtgga
    25261 aaaaggatga ttcaaacatt tgcaaaaatg agtagaagtg cttggattga aaagctggaa
    25321 aatgatttga gtgttatgcg attagctcca aagaaattca aacgagagat tgcagaaaaa
    25381 gaagcaattt tgtcggtatt gaatgcggta gaactggacg aaccgaaaaa gtcagttgat
    25441 gtacctgaaa atgtagcaga gttactagat tattatcgta attcgtcaga tgtcgatttg
    25501 ttagctctga tattaacatt tcacgattgg tattactgtc aaaatgaaga tactcagcat
    25561 gataaacatg ataaagcagt tgactggtta atagagaatc cagaaaagtt catgcgtgct
    25621 tggctagatg gctatgatag caagaaaaag ccactggaaa gtaatgagga aacgtattta
    25681 acggcagaac aatatgacta tgctagaaaa acaggagatt gggaatctgg taagactttc
    25741 aatcacagaa aagatcaacc aaaaattagt ttttcagata tagccgcaat tatcgaaggt
    25801 atctactatg gacaagccga tgaagagatc ttttctatac aagatttatt agaatttttg
    25861 aacaaaaata aggatagctt tgtagaacta actgattatg aggaggatac agcaaatgga
    25921 aaattatgat ccaaatatgc gatggggtac acatacgctt aaagtttctt ttcaacgttg
    25981 ggattacaag ggatttgtga cttttagaaa agctggaaat tgtaaaggat tagatgtctt
    26041 agcgttggat gaagactatc tatatgacca tccattgacg gataatccaa tcggatttgg
    26101 attgcttcct gaagatgatg agggcaatga atggttcaaa atgattctta caaacgataa
    26161 aggtgatcaa ttatttgtag aagatacttg gtcatatttg agtgaatata ttgttagcat
    26221 taaaatcatt gattttgtag cagataaaga aaaagagatt ggagaaggta aatctaatta
    26281 ctagctaatg aatagcaact ttggaggata agtcaaaaga ataaaaaaag aatactgtgt
    26341 gtcattgcag ggatgtatct ttgcatgata gtatctgcgg ttaccattta ttggttagga
    26401 aatcagcaaa acagtttaat tgttgatctg ggggagttaa aggttggatt acgaatgact
    26461 agatggcttt tattagctca aacagttggt ttagccatat tgacttggat atgtgtggaa
    26521 aaaataacat ctaataataa tcaagagatt gttgaatcaa caaacctaag aataagaata
    26581 actgaacaag caattgaaaa gcaaaaagac aaagaaaaaa aggaaataat gtcaatttat
    26641 ttatctagtg gagcatcatt ttttcatgaa atagattcga tgaaactgag tagtcaagaa
    26701 atcattgata gatggaaaaa catgcctcta ggtggtgagt taatcgttga agatagtgat
    26761 ggttggtcag cttataagaa ggacgagatt gtagctatca cgattttgtt tgaatagtaa
    26821 tatattacca ctagataatc tgaatatgtt gatctgctag tgacgtacca aatgcgttta
    26881 atagaacctt ggtcacaggc agaaattgat aaagcagtaa aattgaaccg attatcaaaa
    26941 tttaacgtat taggaaatgc cttttaaaaa tcgcaataca caaacagaaa aataccaaaa
    27001 aattagcaat gaacgtaggt aagaacatga caataaataa taataaccct aacaaatata
    27061 atgctcttgt ccaatgctat atcgaaaaca aaattacaat gcaagaagtc gctgatgaat
    27121 taggcaaatc aaaaagccta gtatcacacg actttaagca caatgttttt ccgaacccga
    27181 ttgagctgag gaaaaaattt caaaagagag tttatcaaga aatagggaag tggattgatg
    27241 aagggaatgc aattgaccct ttaattgaaa aatatccttt tttaaaaagt aaaaataaaa
    27301 ttcaaaggtt aattcgtcaa ggcgatattc aagctaaaga acctgttttg acaaccaatg
    27361 atttggctaa aataaatctt cataaacaga ttattgaaga ctttgaaaaa aatctttcaa
    27421 caactgaaat tttaaaaaaa tacaatattc cgagaggaaa gctaacatcg actcttttta
    27481 gagaatatgg agaatcggca gttactaaag caaaggaagt agcgaatgag agaaaaaaac
    27541 aaaaatagta gtcgatacct agtagcgact gtagtttttg tttgtgtatt gattttcctg
    27601 tttgattttt tgcagattgg aaaaagcata attggaaata cattgagtgc aaaggaaatg
    27661 gttgctttag gtggtttgta tgaaggacat ttgttcccga atatattcat ttctatcgtt
    27721 tctcatatta acttgtacca tttaatttct aatttattgt tcttagtagt tcttgaagaa
    27781 gcactgaatg atcaatatga gtggttaaca ctattgattg ttttaatcat atcaggactt
    27841 ctaggaagct tagttccttt tctgttacaa agcaacatcg taagtatggg gcttagtgga
    27901 ggtatttttg gatggattgc tttatttgcg attgcataca tccgctttga tagtaaggaa
    27961 catttattac taagtgtttt tatattattg ataatcggca ttttactggt atctactttt
    28021 gaatcaggcg agacaaacgt cttgtcacat gtaatcggat ttgtctgtgg gatgatcttg
    28081 ggatttattc taaagaacat ttcattgatc aagcaaaaga attaaaaggg agtgctaaaa
    28141 atgccaaaaa aaaaagaaaa gaaaattcca gtgtttttgt ctgtctcaga aatgttatca
    28201 cttcaaatac tgttaagaaa aaagattgaa acaatagacg aagaagttag aaataaaagg
    28261 gctatgtacg aacaacgtga agcgctaaga gaacgaatcc actattttga tgatatacac
    28321 actaaaatct atgaagcacg tacaaataac gaagacgaaa gcaaattagg attagctcaa
    28381 caacgaatat ttgaatggct aaatacgaat actgagcaag aaccgttaag agcaattagt
    28441 aatctttttg aatttggaac gaagcattgg tttaacgcag tagaaaagga tgctgactta
    28501 caagaagcct ttgacacgct aagtaaggat agcaaatcca tgcgagattt attgattact
    28561 tatctaaatg agcatgagtt tgaacaaacg aaaaccaaaa agaaaaataa agaatagaaa
    28621 gttgtgatga atcatgctat taaccctgtt aggttattta tgtttaattc ttgctttttt
    28681 tggggcaatc gctataatta ttggtcaatt tcaaacaaat aatccgatta tgcgtaacat
    28741 aggcattgga attgtgattg taacctttat tgtgtttaat gccactaaga tttttggaag
    28801 taacgataaa caatttggta tatcgggagt taataatagt tatgctccag attctttggg
    28861 aagggtagta atagatggaa catttgaaaa tgtaacgagt tataaaaatg tttcgctgac
    28921 agtgaaggaa gatggcaaga aagcatcaga cgacgttctc gctactatta agccgtctca
    28981 atcacaattt gaagtgtttt atacagttaa agagaatagc cctgtacaca agctgacatt
    29041 ggtttttaac gttgatggag aagaatacta taaagatgta aaaataaata aaacatcaac
    29101 gaaaaagatt atcgaatcag cgaaaaaaga agtagctaca gaagaaagta caactgaatc
    29161 aagttctact gtcgaagcgg aactaccaga taaaattgat tttgatcctg aaaaagtcaa
    29221 atcgtttgaa tctgtttctg gttctatggc tgatcggttt aacactgaag tcttcaaagc
    29281 tatcaaaact gttaaattgg atactgaaaa gcaaatgatt aatgttacct tattcaattc
    29341 agttgaatct atggatcagg ataaaaaagt tacggttgca agagaactag gaaaaagagt
    29401 aattggtttg tatcgtggta ctgctaaacc agattcagac ggtgcagatt tgccagttca
    29461 tgtagtttat gaagacggta aagtttttgg agattctaca gcaaacgaag cattaacgat
    29521 taaattggct aaatagccag aaagttataa taaacaaatg gtgtcacacc aataatatat
    29581 aggtgtgaca ccttggagat gatagctgat ggtagtaaat aaagaaaaag ttagaaagta
    29641 tgtgacttta aaaatagctt ctgttaaata tttagaaaag cgagctaaag aagaagaaag
    29701 agcaagaggt aagaaagtaa cagtttcaga gttgttagaa gaattgatcg agaatgatca
    29761 aacaataaga cgtgcattta ctaataggtc tatcagataa caatataatt aataaaaaag
    29821 aaagagtgat ttggtcttag actaaatcac tctttttata taaagtttct ttacactcat
    29881 tccgaataat gttaaaattc tttatatgat atatagttcc ttgtcagagg aagagataac
    29941 cagcaatatt ggagggataa taagtggaaa acaataatga tattagacat tttagcattg
    30001 gggaaagagt tacaacagta agtgaattat caaaggatta tagagataaa gagggaacga
    30061 ttatttctct taataagaat cgaagaggcg tttggcaata tgttgttgat ttaggtgatg
    30121 gcgttacagt tgagtgtaaa gatagtcaac tgatcagaaa taatggcagt cattccattg
    30181 aaaaagcaat tcttgtatac ccaaaagtat caagtaagat catgaatgac tggttgcttg
    30241 gaaaatttgg tgagtacaat aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa
    30301 cagtattagg gtatctaaaa aagataacga gtaaagaagc aaaaattgag gatgttttaa
    30361 cagtaagaga gcttcaatca ttaatcgagt atcggaataa acaaataaca aatcctgagt
    30421 accttagaaa agaagatggt ctattgaaac aattggaaca agtaaataaa atccggaaga
    30481 tgcaaaaggt agctccttta agccttgata cctttaaaaa aatctatttg aaagagatca
    30541 aaaaagacta atgtggactg taactttacc gttccaacct gtatctgcac caagaccaag
    30601 tgcaagaagg aatgctgctg atgaaactaa tgacgtagca gagaaaagga tttcaaccta
    30661 ctatgataaa aagtatcttg attatttgaa aaaaataaag agctttatcg aagatcaaga
    30721 tttattagat gatgaatttt actcgattgt aaatgatcct atgggcgcaa ttatggaagt
    30781 ggacttttac taccagatac caaaaagcta taaaaaagtc tataacatta tgaagacaac
    30841 agcgccagat ttagataatt tagtaaaaag tgctatggat ggtattttta acatctcagc
    30901 tatacgagat agccatatca caggcttaat tgcttttaaa tataatgtgg cgaacgaagg
    30961 aaaaacagtt gtgaggatca aacgatattc agaatttgag tgggatactt ctgaaggatt
    31021 gaacggagat atatgggaag ctacatttga ttttaaacca gtagcaacgc caagaccaaa
    31081 gtctaaattt agaggaaatg ggattatcac ttactatcca aaagagtaca acgactattt
    31141 ggaaaatatc aagaatacgc ttaaagagaa agatttggta aatgatcaac tgaaaaaggt
    31201 aatgaatgct cctatggggg ttatagctca gatagattat ttttaccaag tgagaaaaga
    31261 taagaaaaag cttgattcac tgatgcgtac ttcacagccg gatattgata atttagtcaa
    31321 aggaacattg gacggaattt ttaacaagga aataggcatc aaagatagca gggttgtagg
    31381 gttaattgct tttaaataca atacttttga aaagtcgaaa acaaacgtga gactacagga
    31441 gatgggctga tgaataataa tgaaaaaatt cttcatgtac ttgatagctt tgaaataatt
    31501 caagaagaac taaaaaaata tcgggatgtt ttggaacaaa gatatgattt tgtcaatcag
    31561 caaaagagta atcacatgga tttcattctc aatatgaatg acttgaagaa gaaattagtt
    31621 gaacgaaaag aacaagaaaa gttgatcaaa gcttactttg aactcggtga aaaagaagtt
    31681 aaaaatgcaa tggaactgaa tgaagagcgc agggttttag atcagctgtt agaacaatta
    31741 ttggtgatgt ttcaaaaagg acgaatagat gaggatttaa ttgaagaagg attgagaaaa
    31801 tatccttcaa actcaggtat cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa
    31861 gctttcatac cgccagagga ttttgaatcg gcaatggaat atataaaata ttatagccaa
    31921 gggatcactg cttttagaga gtttgatcct gaagatgtta ttcatgactt gaacaaccta
    31981 aaagaatggt gtgaaagtta tggagtcgat gattccggat tggactactt gatcagtatt
    32041 atggaaatcg aagaagagat gccggataag cctgatccaa cagatatttt agaattgatc
    32101 catgaagcta gaaatccaat tgcctatatt tctagaggat atactgtatt ggaatactat
    32161 aagccttata tatcagcgat gaaccattta agacgtgtat taagggaaaa acgtgaatac
    32221 cgctcagtgt tgaatgcaac gaatagatta gaaaaagctg tttcagagct agatgcttat
    32281 tatcgtgaac attacctgca agcaggtggt atgccaagaa atacgaaagc aaatatcagt
    32341 aggtatataa aaaaagctga gtaggcggag ttagcccata aaatggcaaa aggtaacctg
    32401 aaaggggcat tttgaatgaa aagggatgtt tttgaattgt acgtattaga acgtctagaa
    32461 gacggcatga tggcacaaga gataattgat gaaattgatg atatgatcag cagatttggt
    32521 caattggagt tgaatgatgg aaacaaaaat tcggtagtgc taaaacaatt ttggttgaac
    32581 aatttttcgt ataaggattt tatcaaagaa aaggcaaata cattgtactg actaaattca
    32641 cgaggagcag tgatgagagc ttattgctaa gagcagttag gctctttttt gtttgtgtaa
    32701 aattatttgt tttataatac acatttatac acaaaaaaat attgacagat agtacacaaa
    32761 tgtggtagga tatatttgta agcaagttag attaacaagt taaaggaaga gcagttatga
    32821 aaaaagaaga actaatagat atatttcaaa ttgttgaacg agcaaataat atgggaatta
    32881 tgttttttga tcgtatttct ttaaagatg
//
LOCUS       DAZKSR010000022        32849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000022 DAZKSR010000000
VERSION     DAZKSR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.022"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..842)
                     /locus_tag="ACG8XG_000897"
     CDS             complement(75..842)
                     /locus_tag="ACG8XG_000897"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0772830.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(835..1695)
                     /gene="ylqF"
                     /locus_tag="ACG8XG_000898"
     CDS             complement(835..1695)
                     /gene="ylqF"
                     /locus_tag="ACG8XG_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0772831.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            1955..2353
                     /locus_tag="ACG8XG_000899"
     CDS             1955..2353
                     /locus_tag="ACG8XG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0772832.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2590..3132
                     /locus_tag="ACG8XG_000900"
     CDS             2590..3132
                     /locus_tag="ACG8XG_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0772833.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(3215..3766)
                     /gene="lepB"
                     /locus_tag="ACG8XG_000901"
     CDS             complement(3215..3766)
                     /gene="lepB"
                     /locus_tag="ACG8XG_000901"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0772834.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(3918..4145)
                     /locus_tag="ACG8XG_000902"
     CDS             complement(3918..4145)
                     /locus_tag="ACG8XG_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0772835.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(4142..4660)
                     /gene="msrA"
                     /locus_tag="ACG8XG_000903"
     CDS             complement(4142..4660)
                     /gene="msrA"
                     /locus_tag="ACG8XG_000903"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0772836.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(4680..5393)
                     /locus_tag="ACG8XG_000904"
     CDS             complement(4680..5393)
                     /locus_tag="ACG8XG_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0772837.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(5396..6292)
                     /locus_tag="ACG8XG_000905"
     CDS             complement(5396..6292)
                     /locus_tag="ACG8XG_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0772838.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(6450..7292)
                     /locus_tag="ACG8XG_000906"
     CDS             complement(6450..7292)
                     /locus_tag="ACG8XG_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0772839.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            7424..8077
                     /locus_tag="ACG8XG_000907"
     CDS             7424..8077
                     /locus_tag="ACG8XG_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0772840.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(8140..8667)
                     /locus_tag="ACG8XG_000908"
     CDS             complement(8140..8667)
                     /locus_tag="ACG8XG_000908"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0772841.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(8687..9634)
                     /locus_tag="ACG8XG_000909"
     CDS             complement(8687..9634)
                     /locus_tag="ACG8XG_000909"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0772842.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(9646..11529)
                     /locus_tag="ACG8XG_000910"
     CDS             complement(9646..11529)
                     /locus_tag="ACG8XG_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0772843.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(11692..13770)
                     /locus_tag="ACG8XG_000911"
     CDS             complement(11692..13770)
                     /locus_tag="ACG8XG_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0772844.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(13783..14058)
                     /locus_tag="ACG8XG_000912"
     CDS             complement(13783..14058)
                     /locus_tag="ACG8XG_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772845.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(14420..14875)
                     /locus_tag="ACG8XG_000913"
     CDS             complement(14420..14875)
                     /locus_tag="ACG8XG_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772846.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(15029..16237)
                     /locus_tag="ACG8XG_000914"
     CDS             complement(15029..16237)
                     /locus_tag="ACG8XG_000914"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0772847.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(16349..16681)
                     /locus_tag="ACG8XG_000915"
     CDS             complement(16349..16681)
                     /locus_tag="ACG8XG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0772848.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            16829..17704
                     /locus_tag="ACG8XG_000916"
     CDS             16829..17704
                     /locus_tag="ACG8XG_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0772849.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(17786..18367)
                     /locus_tag="ACG8XG_000917"
     CDS             complement(17786..18367)
                     /locus_tag="ACG8XG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0772850.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(18373..19632)
                     /locus_tag="ACG8XG_000918"
     CDS             complement(18373..19632)
                     /locus_tag="ACG8XG_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0772851.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(19634..20680)
                     /locus_tag="ACG8XG_000919"
     CDS             complement(19634..20680)
                     /locus_tag="ACG8XG_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772852.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(20915..21190)
                     /locus_tag="ACG8XG_000920"
     CDS             complement(20915..21190)
                     /locus_tag="ACG8XG_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0772853.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(21433..22743)
                     /gene="der"
                     /locus_tag="ACG8XG_000921"
     CDS             complement(21433..22743)
                     /gene="der"
                     /locus_tag="ACG8XG_000921"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0772854.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(22925..24154)
                     /gene="rpsA"
                     /locus_tag="ACG8XG_000922"
     CDS             complement(22925..24154)
                     /gene="rpsA"
                     /locus_tag="ACG8XG_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0772855.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(24289..24969)
                     /gene="cmk"
                     /locus_tag="ACG8XG_000923"
     CDS             complement(24289..24969)
                     /gene="cmk"
                     /locus_tag="ACG8XG_000923"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0772856.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(25018..25653)
                     /locus_tag="ACG8XG_000924"
     CDS             complement(25018..25653)
                     /locus_tag="ACG8XG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0772857.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(25719..27122)
                     /locus_tag="ACG8XG_000925"
     CDS             complement(25719..27122)
                     /locus_tag="ACG8XG_000925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0772858.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(27109..28140)
                     /locus_tag="ACG8XG_000926"
     CDS             complement(27109..28140)
                     /locus_tag="ACG8XG_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0772859.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            28171..28392
                     /locus_tag="ACG8XG_000927"
     CDS             28171..28392
                     /locus_tag="ACG8XG_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0772860.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(28557..29294)
                     /locus_tag="ACG8XG_000928"
     CDS             complement(28557..29294)
                     /locus_tag="ACG8XG_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0772861.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(29447..30787)
                     /locus_tag="ACG8XG_000929"
     CDS             complement(29447..30787)
                     /locus_tag="ACG8XG_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0772862.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(31024..32547)
                     /locus_tag="ACG8XG_000930"
     CDS             complement(31024..32547)
                     /locus_tag="ACG8XG_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0772863.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         9202 a         6859 c         5551 g        11237 t
ORIGIN      
        1 tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat aagatacgta
       61 aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt acgatggatt
      121 ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata acctgcatta
      181 tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg gtctcgatat
      241 actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc tttgattatt
      301 ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata gcttggtaca
      361 atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata aatattcaca
      421 tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt ttcttgaact
      481 tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt cagccctaat
      541 atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc taatggtcct
      601 cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc ttgcttttca
      661 aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa tcgtcgttca
      721 aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga ttctagtgta
      781 ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat ggattcagtc
      841 atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa gttttccttg
      901 acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat acccacgttt
      961 tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga ttgataaacc
     1021 ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc cgtaaatcgc
     1081 taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta gttttttgcc
     1141 gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca attccaactc
     1201 attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc ctgtctgtgc
     1261 aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg gaatacctaa
     1321 gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg cccgtttttc
     1381 tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt cttgtgccgt
     1441 gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct ttgtctgttg
     1501 tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct gaatgatctg
     1561 atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt caaatacgat
     1621 gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat gtcccggaaa
     1681 ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc cggctttact
     1741 tgtttttttc tttttcaata taaattcact tttttattat aaagaaattc catctattag
     1801 ttaagtgaaa gtgaaccagt cctcattcta tcacatcttc acttttttga tgatgcaaat
     1861 ttttattttg gtttctatat atgaatctaa atttttgaca gcgtttccta aataagagat
     1921 gatagacgaa caaaacagaa aggagacgaa acaaatgaat agtatggttt ttgttaattt
     1981 tcctgttgct gatgtcaatc gttccgttgc tttttatgaa aagctgggtt ttaaaaaaaa
     2041 tgaagaattt tctactgaag aagctggttc aatggtatgg gatgacactt tttggataat
     2101 gcttctctct catgattttt ataaaaaatt tataaaagat aaagaaatag ctgataataa
     2161 aaaaacaagt ggtgctttga tttcgtttag tgttgaaaat gcggatgaag taaaaaaatt
     2221 tgctcaaatt gcaaaagaaa atggtggtac tttccacgaa gtagatatgg gaatgcctga
     2281 agaagaaatg ttttcattag aagtccaaga tctagacgga aatacattag aacccgtatg
     2341 gatgaagatg taagcagata actaatctga cgttttttat aacaaaaaaa caaaggcgga
     2401 tgatacgata agtattctcc gcctctattt tttttattcg tacaatctct tttttacagt
     2461 ttataaaagc agtcttctca cccttttttt aattgttaga tgccagtgta tgtaaatcac
     2521 caatgtgtca tgacacattg gtgattttat ggtatccttt tattattaac tgacaaagga
     2581 gagaatattt tgaacacttt gattgcaaaa gacgttttta gattgcccga aatgaatgca
     2641 agattgttgt cgacctctgc tcttttatta gcccttgatg tcatcttata caagatagcg
     2701 attggtcctg cttattttca aatcacattc ggatttttaa gtatggcgct tatgggttat
     2761 ctatatggac cgatctgggc tggtttgctt gaaatgttgt cgaacacttt aaatttcacg
     2821 atatttggtt caggaacgtt tcaagctgct tttcttatca cggctttcct tggtggattt
     2881 attaatggct tatttttata tcataaaaag ataaacctgc cttccgtcat cgctgcccaa
     2941 ttattgatta tgatcccagt cagccttttt atcaattctt ggctgatgtc tgttttgttt
     3001 ggtgcagatt tcaaagtaat ttttctaggt agattcatca ggaatatgat ccaaatccct
     3061 attcaaataa tggttgttta ttcatttatc aaaactttag aggctagaaa agtgtttaaa
     3121 cgagctgttt gattttacgt actaaggtag taaactgatt ttggattaaa caataaagcg
     3181 tgcagtctca acagactgca cgctttattg tttctcactg gatccatttt atttcatcaa
     3241 gtggataata gacaaatctt gctttaccta gaatatcagt tttattgatc gtgccaaacg
     3301 aacgactatc ttttgaaact cttcgattat ctccaagtac aaaataatta tcttccggaa
     3361 gcttggattg tcccgttagc tcttgtagtg taaagtccgt cgtataggat gcatttgcat
     3421 gatctgattt caggttttta gttagataag gttccttaac tactttccca ttgatcttca
     3481 attgatcatt ttggtagctg acattttctc ctggaagacc gattactcgt ttgatataaa
     3541 tcgtgccatc tgctaattga aaaacaacaa tatcaaatcg tctgatctcc gaaaattttt
     3601 ccatcacaac catatcacct tgcttcaaca cgttttccat tgaattgcct tccactggta
     3661 ccgggatgaa taaaaatccc cgcaacatga acgcaaggaa tacagaaaca agtaagtatt
     3721 tgaatacaag ccagaagcga ttaatatagc gttgtttttt tgtcaatctc tatcaatcca
     3781 tccctctttt tttcattctt tctttattat aagaaaaata tcaaaaaaat gcgactagat
     3841 agctgaaata aaaataattc tttcaattag gctattggac aaaaaacttt tttgatcata
     3901 aagtacaaaa ctatctctta tatgtcatga ttattttcta aatatttttc ccatgcttcg
     3961 tcaaagatat ccatggacgg caaatagtcg acattcagtt ccagataatt agaaatttct
     4021 tcgtagctag agctgtgctt aggaaattgg atgtctttac cggcttctgt agcgaaaaca
     4081 gataaagcat catgtgtgcc accgcctcgc agtgtcatta aataatgaaa aaagcttcga
     4141 ttcacccttc gttcctccaa ttttcttcga taaaggcagc ccggcgtgca tggtttcttt
     4201 taaaattttc aggatgcttt ttataatact cttgatggtc ttcttctgct agataaaatg
     4261 gttgggctgg ttcgattttt gtaacgattg gctgttcgaa acgtccactt tcagctaact
     4321 gtttcttact tttttcagca atgatccgct gttcttctgt caaataaaag atcaccgggc
     4381 gataattgtc accgcgatcc tcgaattgtc cgaaagcgtc agttggatca gtttgctgcc
     4441 agtaaagttc tactaattgt tcatatgaaa tcaatgttgg atcatagata atctccactg
     4501 cttctgtatg tccagtcgtt tggcttttta cttgttcata agtcggatct ggtacatgcc
     4561 ctccagtata tcctgacatc actgtatgga taccaggaag cgtgtcgaaa ggctggatca
     4621 tacaccagaa gcagcctcct gcaaatattg ctcgttccat tatgattccc cctctaatac
     4681 tactcatccg atgatttttt attttctttt gtcttgcttt cagtactgct ggtgctagtt
     4741 ttgttggtat ttgagttgtt tgaagcatca gaggtatctt ttggcaaata tagactaaac
     4801 cgaatgtcat catttactaa gtcgatttta tctgctttaa tatacatccc gttttgcatt
     4861 cggaattgat ccaacctaag catgatagtc agatcatctg tattgacttc gatccaatct
     4921 ggtaatttat agtttctctt gatcatattc atcacatctt tgattggcaa actcaatgtt
     4981 ccgatagaaa gacttctagc ttttagttgg acatttccgt tttccatcac atacggatca
     5041 aaatataaat agaaatcgat aggaaaacct agtacttcga aagtaccatt gagtagtgct
     5101 tcattttcca aataaaattt atattcaata tcactatctt tttgatactc actaaggaaa
     5161 aagctgatga tttgattgac tttttcttta tttgaattga tcgttaagac aggatctcct
     5221 tgtctttccg taagagctgg tatttctggg agttctggtt cccgattagc aaaaatacgg
     5281 ctgcctaaaa atacggtcga accaagtact accccaagaa ggataaggaa ggcgaatttc
     5341 cagtaatttg ttttctttgt tttgtttgaa gatacgtcag atcttgtttt catgtttatt
     5401 gatcatcctc cttctctaac cattggtttt gtgtctcgat cagctgatct ctcactgcat
     5461 ttgccatcaa ttgataacct aaattatttg gatggaaatg atcttcttca tagagcacat
     5521 tatttttgat gccatttaga ttttctgtcg tgctagttgt ttgatctgtt ccttcttctg
     5581 tgacacctac ttcctgattc aatccttgat aaagcaaatc attgattggg ataaagtagc
     5641 agttcggcat tttttttgtc accgtttctg ttcctttgtt ccaattgtta acaattgtct
     5701 gcatgtcagt gatctcagga aagttcaaat aaaatggatt gtagatgcct aaaatgtaaa
     5761 taggtgcggt tttattgagg gatcgaaatt cttccaatag ttctgtgaca ttttcctgat
     5821 agtttttcag tggcttttta aaggaattga ccgttaaccc gaataaatta ttttggataa
     5881 ctttcatcaa atcattaccg ccaacagtca gtgtgataaa atcagctgtt ttgatattgt
     5941 ttcgaatttc ttcttttgtt tttactcgct ttaagatctg gtcacttcgt tcaccattta
     6001 caccatagtt ttctgtttca accgcggtca aattataacg gtcttttaga tcatttgcga
     6061 caaggggaac gaatcctcct tgcttggttt gatctcctac tccttcagtc agcgaatctc
     6121 cgatagctat gtagtgaagt ttttcttttt tatttggatc ttctctcaca gtagttactg
     6181 gtttcattag tgggccagct ttcggtatag aagcagctaa acctgcatag atgatgatcg
     6241 caattaataa tgggaacaaa acgaggatca aatgtcgttt cgttaatttc attttttcac
     6301 ctcttgtatt cgattggttc taatgttatc aaaaatttta tcaaaaaggt agtctttatg
     6361 aaaaaagaaa acaattattt tcctaatttt ataaagaaaa agagaataag ctgcaagcct
     6421 ttcaggctga gggcttatcc tcttttcgac taatctacat aatacatgat tgcaaacgcg
     6481 tctttaccag tatgcgtagc gataaccgga ttcgtatgca gtacagggat atgcatgtcc
     6541 gggaataatt cttgcaaacc ctctttgaat tcttgcgcca attcttttgc atccgcatgt
     6601 gaaataccga tttgtcgcac atttggcaat tctttcagtt catttttcaa attattaaac
     6661 catttatcga acgttttctt tcctcgcccc ttagttacag ggattaattc agaatgggcg
     6721 aaattcatca ccacgcgtat gttcaaaaca ctagacagca aaccagttgt tcggctgatt
     6781 cttccacctt ttaccaagtt atctagtgtg gatattccga tgtataattt tgtattgtct
     6841 tgtacttttt tgacttcttc aaggattttc tctactgttt taccttcttt tgctaattca
     6901 gctgctcgaa ttacctgaaa agataatcct tggtcagtgg tatcactgtc gataaccgtt
     6961 actttcgttt ttgttagatt actggcttgt cttgctgctt ctactgttcc actcaagcct
     7021 tttgtcatat ggatcgaaat cacttcactg ccgtcttctc ctagctcatc atacatcgag
     7081 atgaattctc caattggtgg ttggcttgtt ttgggcagat tttttgcttt cgccatcatt
     7141 tccataaatc tttcgccgtc tagttggtca tcatcgggat atactgttcc gtcgatcatg
     7201 atggatagcg gcatgacacg aatgcctaaa gcatcgcgta catttttttc catcgtacat
     7261 gaagaatctg ttacgatttt tacatttgtc attattttct ctctctcttt ctttaacaag
     7321 atgccttatt catgttaaac tacatgtaag acaatttcta tttttagtat aacagacaaa
     7381 taatgatttt tcattaatta cacctagtaa aggagccgtt ttcatggaaa aaacgaaatt
     7441 taatcgaacg tatgaaatat taaatgaagt gtttaatgca gtcacccatg gaattgggac
     7501 tggattgagt atcgcgggac tagtggttct tttagtaaaa ggtgcccact tgcattcctc
     7561 cctccatatt gtctcttatg ctatctatgg atcgatgatg attcttttat ttttaacgtc
     7621 tactttgttt cattcattga tttttacaaa agcaaaaaag gtgttccaag tattcgatca
     7681 tagttcgatt tttcttttga ttgcaggaag ttatactcct ttttgtttgc tgagtatccg
     7741 aggatggcta ggttggactt tatttgtatt aatctggttg tttgcgattc ttggcattat
     7801 atataaagcg ttgacgttgc ataaacggga gagagtatca aaagtatcta cgatcatcta
     7861 tatttttatg gggtggctct gtgtcgtggc tgctgttcct ctttatcact ctcttggaat
     7921 gactggtgtt attttgatgg tagcaggcgg gctttcttac acgataggcg cattctttta
     7981 tagcttgaat tctatccgct atatgcatgt tgtttggcat ttgtttgtta tgttaggtgc
     8041 aggttttatg tacttctctg ttttattctt tacttaataa aaaaaataaa gctatggatg
     8101 ggacagctct aaataacagc tagtctatcc atagcttttt tatatcgtca attcgcctgt
     8161 cccgcttttt acaggattct cattacgctg atacgtttca aattgatgcg gataacgatt
     8221 tttttcatcc atttctcctt gactagtatt gatcagtgac caatcagacc agtcaacagg
     8281 tggaaaataa gcatctccat cgaatacatg atggatgact gtacggtata atatcgtaca
     8341 aaatggcaga aattcttgat aaacagctga ccctccagcg atgaaaaaga taccgtctgt
     8401 ttctttttcc agagcaagca cgtcttccac actatgcatg acatgcacgc cttctgcttt
     8461 ataggaagaa tctctcgtca aaacaatcgt ctgacgattc ggcaacggac ggctacccat
     8521 cccttcgaat gtttttcgtc ccatcactag tgtatttgct tctgtcattt gtttgaagaa
     8581 tttcagatca tttggcaaac gccaaggaag acggtcttct tttccaatca atccgttttc
     8641 atcctgtgcc caaattgcag ctaacatttt ctcatccttt ctgattctaa acagcgatag
     8701 gagctttgat cgccggatgt gggtcatatc cttctaattg gatatcttcc atctcaaaat
     8761 caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt atttctcggc
     8821 tcaattgttc tttcatttgt tcgatatgat tgatataaag atgagcatct cctaatgtgt
     8881 gaacaaattc gccaaccgct aaacctgttt catgtgcaat taggtgagtc agcaacgcat
     8941 aacttgcaat gttgaacgga acgcctagaa acacatcccc actgcgttga tacaattggc
     9001 agcttagccg tccctcttga acataaaatt gaaacatcgt atgacaagga ggcaaagcca
     9061 tgctaggaac atcttccgga ttccatgcag aaacgatcaa tcttctagaa tcaggagtct
     9121 ttttgattgc ttcgatcact tgtttgattt gatcgataaa gccaccgtct tttgtttccc
     9181 agtgacgcca ttgatagccg tagatgtgtc caagatcgcc gtatgtttca gcaaaggaat
     9241 catcggttag gatcttctcg caaaaaagct tgctctgctt tttatattct tcattgaatt
     9301 caggatctgt caaacatcta tggccaaaat cagtcatatc tggtccatta tagtctggac
     9361 ttttgatgta gcgttcaaat gcccattcat cccaaatatg gtttttattt tgtaatagat
     9421 agcggatatt cgtatctcct tttaaaaacc aaagcaattc gcttttgatc aatccaaaag
     9481 gtactcgttt cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc atttggtatc
     9541 cgaataagct taatgtgcct gtgcctgttc tgtcttcttt acgatgccct tcttccaaca
     9601 attttcttcc taagtcaaga tacgcttgtt ccatcctttt tcctcctaat cttctaccca
     9661 ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg tttcagatac
     9721 gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca tctcttcttg
     9781 gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt cccattcttt
     9841 ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta tttttggttt
     9901 cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg acatgtttcc
     9961 gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg ctaccttgtc
    10021 caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc taaaataatc
    10081 ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca ataataaaac
    10141 attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc cgcctgaaag
    10201 tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa gtaattctgc
    10261 tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt gtaagtaagc
    10321 aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg caagatgaac
    10381 cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta gaatattaag
    10441 caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc gagattgaat
    10501 caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc catctttgat
    10561 ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat catcttcttg
    10621 gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg cttgctgctt
    10681 ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat aaagccgctt
    10741 gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta agacgtaagc
    10801 ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga ttcggtttgc
    10861 tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac ggtattgttt
    10921 caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg gttcgtctag
    10981 taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt ttttctgacc
    11041 acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt gcaaaataat
    11101 ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt ctgctttagc
    11161 ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca atgcttcctc
    11221 atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt ggagaacagt
    11281 taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat cagttggata
    11341 aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat ttaagagact
    11401 tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt ggaccaaaaa
    11461 agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag ttacttttaa
    11521 ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag tgaacttcag
    11581 gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg accgattcga
    11641 tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt tttattttat
    11701 tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt tatcagatgg
    11761 tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt tcgcagaaaa
    11821 cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa gataataacc
    11881 tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag aaatcaaatt
    11941 ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt gaaccatcgt
    12001 ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag tatttttttg
    12061 atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac gatcagcata
    12121 tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac ggacaacaat
    12181 gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga atcgagaaag
    12241 aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat aactttcatc
    12301 caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt cttgcttttc
    12361 atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat tttctatcac
    12421 tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt cttttcgcaa
    12481 aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt tcgcttcctc
    12541 tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta agatgacacc
    12601 cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga ttgaaaatgg
    12661 caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg ttaagatcaa
    12721 gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat aaaaaagact
    12781 gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa tcgctaataa
    12841 aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg tcaattcgat
    12901 tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc atgtactgtg
    12961 ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag aagcttggag
    13021 aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg ccaatacata
    13081 ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt gtgctgatgc
    13141 tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc cgatcaaagc
    13201 tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa aagatccggt
    13261 gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat tcaaaagacc
    13321 ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag aaagagaact
    13381 gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg cagcaagtgg
    13441 gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca aaatgactcc
    13501 tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa tatctgcttt
    13561 ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt caggttcaaa
    13621 atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa tttggatcag
    13681 tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga tcgttactgc
    13741 aaatacaata actaactcag caaattccat cgtccatccc ctttattttt tattttgtgt
    13801 aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta cttcaacctg
    13861 tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa gcagatcatg
    13921 tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact tacaaagtat
    13981 ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt ttttttgttg
    14041 ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca ttatagcaaa
    14101 gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt agaaatttcc
    14161 ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt aaaacttaag
    14221 aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt tcttgttctt
    14281 ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct tattctgaat
    14341 aaacggtgtg gaagagacag cctatcagta gtaataagac acatctcttt cacagcctca
    14401 tttttgcgta aaaatcgatt tattcgaaaa attccccgta tacatcttct acacgttgat
    14461 ctggattgat cacgatatac aagttacctg ttttttcgct gatttttgtt gattgatatt
    14521 gattgtctaa tccttctgtt cctttatcta gatatgggaa gtacacaaga tcaggtgtgc
    14581 catttcggta aaggatttcc attcgctcga tatcttcgta tttcaatgcg cgattaaaca
    14641 tgcccatttc cagtccgcct aggttgatat catgtgtcgc aactgcgtcc ccttcatgga
    14701 agatttcgat tttaaatcca gcacacggat ggatttcaac aaattcactg ccattgattc
    14761 gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg cggttgatcg
    14821 tccaggtttc tccatttcta aaataaaatt ttagcgctgt catttcttta ctcattaaaa
    14881 agactccttt atgatgtaat ttattcttgt actacctcag tttagcacaa atttatgtaa
    14941 gtggtcacat cttatgtttt ttcttctaat gacgtatctg cccactttaa gtagccaaca
    15001 aagctgagca gatacagtct agtgtttttt aaatcaattt ttctttcgat aaaaaatcca
    15061 aaacctgttt tttatcattt tttattcttt gttctacgac ggctttttca gctaattgca
    15121 aagcttcact aacccactta ccaggttttt ttccagtttc ttctagcaag attttaccat
    15181 ctactgctaa ttcgcttaat gacttgatag gtaaacgatc ataggattct cctacggcat
    15241 ccatctgagg tgtttcgtca aaatagatca tcaattcttc tacttgcagt gcatgttttt
    15301 tgcctaagaa gtacaattcg aatggttcca aagctccagt ttctcgttgt cgtagtcctt
    15361 ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca cttccacgat ttcaaaaagg
    15421 aacgaatctc attttctttt agatccagtt ggctgatcaa taatgcccat gcttggctct
    15481 cttctttcaa tgggacggat ggtaattcag caaaacgtaa aagtgcttcg ccttcttctt
    15541 tcaaaccggg acagcaaatg tagcactctg tttcaataaa tgcatggatt cccgcggccc
    15601 gttcatttcc caagagcatc ttgatgaatt ccacatttat acgttctaca gatattttac
    15661 ctaatagctc gtggttttct ttgatggcgt agaaggtttc ttcctctaat ttgaacccta
    15721 gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg aaccgctcat
    15781 gaggattgcc tacagctcgc aagatctgtt cttctagatc ttgcagacca tcgaacaaat
    15841 cgatgatttc tccatcttct ttcaaagcaa acgcattgat cgtaaaatct cttcttttca
    15901 aatcttctgc caacgaacga acgaattcga catgatctgg tctacggtag tcctgataag
    15961 ttgattccgt ccgaaatgtg gtgatctcat attgttcatc attttctaat accaacacag
    16021 ttccatgttc gatcccaata tctatcgttt ttggaaaaag ctgtttgatt tcttctggaa
    16081 atgcgcttgt tgcgatatcc acatcatgga ttggctggtt cagcaagata tcacgaacac
    16141 ttccacctac aaaataagct tcatatcctg cattctctaa ttttttcaga actggaattg
    16201 ccttttggta ttccagaggt aattgtttta atttcatttt tccaaccttt cctgcgtgtt
    16261 tcttgtcctg agataaagac tcagttgcat ggtgactgtt tgacaagtcc gtttcagagt
    16321 cttcaaggcc aggaccactt catcttgttt actcgtcctt acgctcaaag cgattatggt
    16381 ctctttgatt gaatttttcc atgttttttt caaaaacttc agttaagtcg atattcaatg
    16441 aattagccat gatcatcgta acgaataaaa catcaccaag ttcttctgct accgagttaa
    16501 ctggttcaga agcctttttg cttttttctc cataagtgtg gttgacttct cttgccaatt
    16561 cacctacttc ttcagttaat cgagccatct gagctaatgg agagaaatac cccaccttga
    16621 attgctggat atattcatcc acttcctgct gcatactgta taaagaacgc tttttatcca
    16681 tctttttccc tccaaaacta tcttaccaaa actttaacgc aaatcctaga taagagcaat
    16741 tgagaaaatc gaatttccat gctacattta ggaaggaatt tctaaatgga aattctgtga
    16801 aaatacataa ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa aagaagttct
    16861 ttttatcatt ataggaacaa gtatttatgc atttggtctt gtttacttga acatcgctaa
    16921 tcatttagca gaagggggag tctcagggat caccttgatc ttaagagctt tatttggtat
    16981 tgatccagct tatacgactt tattaatcaa catcccattg attttactag gaggaaagat
    17041 cctcggtaaa cgttccttgg cctatactgt tctaggtaca ctttctttgt cattcttttt
    17101 atggatctgg caaagggttc cactagaaat caatttacag catgatctac tgatcgtttc
    17161 tctacttgcg ggtttagttg caggtgtagg aagcgggatc gtctaccgca tgggtggaac
    17221 aacaggcggc agtgatatta tcgctcggat tttagaacaa aattacggca tcactatggg
    17281 gcgttcccta ttggcatttg atatcatcgt cttacttgct tcattaacct atatcgactt
    17341 gaaacgtatg atgtatacgt tgattgtttc gtatgttttc agtcgtgtga ttgattcgat
    17401 cttagatggc ggatattctg caaaaggtct acttgtcgtc tccaacaaaa gcgaagagat
    17461 tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg gagaaggcgc
    17521 attctctggt aattcaaaaa aaatcatcta tatggtcgtt agctctagtg aattaactga
    17581 agctaagagg atcgtccacg agattgacga aaaagctttc ctttcaatca tcaatgtgca
    17641 tgaagtggaa gggcaaggct ttacttattt gaagccacag acaagattac tgaaaaaact
    17701 ataagaaaaa agacaaagat aggctatgag gaaagattcc acatccagct gacctttgtc
    17761 ttttttagta ctatactatt tttaactaat tgttatgttc acttacccgg ttacttttct
    17821 tttgtgtctg caaaaggatc tttcggttaa cttcatcaga taatttcaaa aaagcatccc
    17881 tatctccatc ttctagtgct tgatcgattt gagcataaag catttttagt tttgcctctt
    17941 cttctttttc ttgaaaatat cgttcaatta attcagtatc ttcttcactg actgaagaat
    18001 cccaagaaga aaatggattg tcttctaaga tagatagata ttctcttgtc tgccaagcat
    18061 tttcgaatat acattccaca tataaaggct ttttccaatt caaacggatt tcatgaaaaa
    18121 tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt tccccttcaa
    18181 cagatagatc actgatctgc agtcctcgat ccgcaaaatt cgccccttca acaaagtgga
    18241 cattgcttaa aatcgcttca tgattggcaa gataattcaa gatccaaaaa acttctcttc
    18301 ggctaaaaga tacatgattg accatccacg ttaaaaaatt cttcttttcc cgtacatcaa
    18361 ttagcatttt tctcacctct ctgttaggtc atctaataat gacagtgcct ccatatctcc
    18421 tggttcaagt gttaaataat aagtcaataa ttcttgcgct cttttcagct gcccttcttc
    18481 tcttaagaaa aaggcatact ctttcaagaa atctggttca tgtgatagtt catgataagc
    18541 ctgttcatag tgtacagctg caacggtaaa gtcttctaat tcattataag catgggctag
    18601 gttccattct gcataaggat tagcagtttc ttccatctga ttgatcactt gtatcacatc
    18661 ttcatatctt tcatcatcca agtaaagatt acttaaagta agtaatgttt catcttgttt
    18721 gtctcctact tctaaagctt tcagtaaaag ttcttctgct cgctgtctat catgcagacg
    18781 gaaagcattt tccgaggcca ggtgataaag ttcgacttgg taaggatttt ctttgattcc
    18841 ttcttctaac actgtttgtg cttcttcaat catttcttct tcttgaagag cttgtcctag
    18901 atatagatat aacgattgat aatgcggatt aacttctcga agttcttcca gatagttgat
    18961 tgcctgctga ttttctttca attgaagatt cgtaaaagct agctggaaca gtcgatcatc
    19021 tgtcctttct tctttcagtg cttcttctaa aggtacgata gctgcttcga attctccttg
    19081 catacttaag gattgtccta aacgttcctg catcgagatt cctgaaattt catttatccc
    19141 gcttgccaac aatgtttcat aaaccactgc tgcctctttg aaacgatcaa ctgaaaaata
    19201 caattcagct agcgcaaact ggatcaatgg ttcatcagga agcaacccag ctgcttcttt
    19261 caattttgct tcactcactt cagggattcc gataacttgg tacaaatcag ctatcgccaa
    19321 taagctttgc ggatagtaat cactttgctt atccactttt tccaggtatt caaaagctaa
    19381 gtcgatctca ttatcttcga ttgcaatttc tgctaacggg atgttcagtc cgtcattttc
    19441 tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa agccaatagc
    19501 cagcaattct tcgcctagtt cagctaatat atctgctggg tcttctttta atgctttttc
    19561 taaaaagatt tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca ttttttcgct
    19621 atttgtattc atttcaattc cctcttatct cactgacgat ttgttgaaat accgtcattg
    19681 ttctcaataa ttctttttcg tttggatgtt ggtatccgtc tattatacct attttttcta
    19741 aaacaagtat ttttttgctt ttttctgcca aaaaaagaac tttttccaaa tactccatct
    19801 ttgaaatctg ttccagccag tggattggat agccaagcaa gtccagccac acaaaaaagt
    19861 tttttagttg gaaatcaaaa ggatatgctt ggagattcca cacgaaatga aacatcattc
    19921 ctaaaaattt tttcatacta gcagaaagta gatggccatt ttcggtcaga taaaaagctt
    19981 gttcaaacag tttaccataa tttgacaggt tttctgcctc taattgataa aaatagatca
    20041 tttcttcgat catcccggca aacggccgag tcgttaattg ctttttattt ggatagtttt
    20101 tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata aaagtcaaaa
    20161 agtcaatctg ctttccgtcc acctgccgct cgctgattgt ttgatcataa acgatgcgtt
    20221 ctggcagatt gatggtttgc agcactacac gatttggtag ctgcaaaatc gtccgctgcg
    20281 gtgttaatgc tcggcttaga gaacggatcg atacagggat catccatagt gatgaatcaa
    20341 gggttgtatg tttttgaaaa aaacctgcca gatccatgat cccttcattt ccaaatccga
    20401 tgataaacat ggattgattt tcgggatagc gtttgacgaa atcaagcagg ttctttaatt
    20461 ctgttaaatg attgcattga gtgttggcac aaatatacca gtcattgttt gcttgatttg
    20521 caaataacgg gttgatctta tctgcaaaca aatcataata gcgctgattc gttaaaaata
    20581 agattggctg atcaatggaa agttctctta gctgactgac tagcgtttct ccgtaaaaca
    20641 ctttcgtcaa cgcatcttct acttgataat caagttccat acgcatccct cctcttctta
    20701 tttttatttt accattagta agcaaattct agaaaagata agagcaaagc tcacctcata
    20761 acttgcaaat aaataaaaat taaataaaat acatgtgttt tctatgtatg acatacggat
    20821 tcggaatctc tgtctcttat ttattccttt tctgctagga aataaaaaga aaagcacttg
    20881 tagccctaga gccacaagcg ctttttcaac tgtcttattt aacagcgtct tttaatgctt
    20941 tacctggttt gaatgcaggt actttgctag ctggaatttc gatttcttca ccagtttgtg
    21001 ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa ccgattaatt
    21061 gaactttttc gccgttagct aaagcatctt gaatagttga aaatacagca tctactgctg
    21121 cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt aattctgctt
    21181 tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata tcatttagtt
    21241 aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc aatattctgt
    21301 tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc acatgctttt
    21361 tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa aaacgtcgat
    21421 atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc cttcaaaagt
    21481 aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca tcaattcctc
    21541 ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag cataaaaaat
    21601 cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat ccataatgat
    21661 atcattcaac acagccgaag agatacgaag attctggttc atacttactt gttcgatcat
    21721 ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga ttggagcgta
    21781 atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg tattggtttc
    21841 tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag cttcatgggc
    21901 gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca gtaccatcaa
    21961 aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt tttcagtatt
    22021 ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga atttctgacc
    22081 actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa tatccgaaac
    22141 aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa catttgggcg
    22201 accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt cagagaaata
    22261 cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg aaacaggata
    22321 aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt ctggattatc
    22381 tactttattg actgctaaaa tcactggttt attacttctg tataaaattt tcgcaaccat
    22441 ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta catctgcttc
    22501 ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat cgccaagatc
    22561 aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt ttgcataaat
    22621 acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac cagcgatgcg
    22681 gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg ttggatttgc
    22741 catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg cattgccaac
    22801 tttcaagcat agattgtcaa ttaaacgaag tatttttatt ccttattgaa atactaatcg
    22861 cttttcaaaa gaaaaactga gacaaaagca gctgctttca tctcagtcac agatacaaaa
    22921 tcaattattt ttcagaatca tcggttgctt tttcttctac ttcttctttc aacgcatcac
    22981 ctaaaatgtc tcccatagta aatccagtat tttcttctgg taattcataa gactcaacat
    23041 tttttggttc ttcttctgaa gctggttttt cttctaaagc tttgatactc aatgcaatac
    23101 gatgttcttc tggatggatc tctaatactt tcactttcac ttcgtcacct tcatgaagca
    23161 cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga gatatggact agcccttcta
    23221 ctccagggaa cacttcaaca aaggcaccga agcttgtcaa acgtttaact gttccgtcta
    23281 atactgtgcc aactggagct tttgtatcga tttctgacca aggtccaggc tgtgtgtctt
    23341 tgattgacaa ggaaacacgt tcttgttcag gattgataga caacactttg acatttacat
    23401 catcattgac agccaataca tcacttggtt ttgctacatg actatgtgaa atctctgaga
    23461 catggactag accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca gtaagacgtg
    23521 caacacgtcc actgagaaca tctccctcat ggatagttga aagaatttct tttttctttt
    23581 cttctctttc gttttccact accgctttat gcgataagat caaacgattt tctgatggtt
    23641 cgatttcgat gattttaaag ctcaatgttt tgcctttata gtctgaaaag tctgcaacaa
    23701 aatgatcttc caccattgat gcagggacaa aaccacgtac accgacgtca acgactaaac
    23761 cgcctttgac aacatttgta acaggtgctt cgatgacctt gccctcttgg aaatctcttt
    23821 cgatatcttc ccaaactttt ttagcatcta aacggcgttt tgaaagtaga tagctgccgt
    23881 tttctttatc tttgccgatc gtactgataa cgactaaatc taatacatca ccgactttta
    23941 ctacttcgct gatatcttct accggcaaag ttgataattc ttttgccgga acgactcctt
    24001 caactccagc cccttcgatt ccgacaatca cttgtttgtc ttcaatgact aaaacttcgc
    24061 ccttaacaac atcgccaact tttacttctt gtacgctttt cattgcgtct tccattgact
    24121 ggttgttctc ttgattttga tcaaattctg tcataacact tgtcctccta accaacattc
    24181 tgccgtaagt acggtactgt ttttagttta attgattatt caaaaaaaat aaagcgattt
    24241 cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt ttcttaattt aaaaaagaag
    24301 ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa tcgatttgcc
    24361 agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt cccttgttga
    24421 atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag tcggaattcc
    24481 tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg ctactaaaaa
    24541 gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt ccatgacaac
    24601 gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga cttttccatg
    24661 tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta aagtcacatc
    24721 ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga taggatactt
    24781 tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg taatagctaa
    24841 ataagttact gcgcgataca tggcaccggt atctgtataa atataattca attcttttgc
    24901 taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg caatactgat
    24961 ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg gctattatta
    25021 tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta agccattcaa
    25081 ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg gaccttcgcc
    25141 tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg tttctgctgc
    25201 attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg gtgttgtctc
    25261 tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag attcttccgc
    25321 gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg tacttgtaga
    25381 actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat tgttgcttga
    25441 tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga ccagtaaaat
    25501 caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag aacttcttga
    25561 tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg tttcgttatt
    25621 ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac tctatcgtgt
    25681 gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat gtttttttta
    25741 aataaattta gtaaaatcgt tccccaatga agttgttcag tcttagaaaa aagcttcctc
    25801 tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg cttttcggag
    25861 agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc attaatatag
    25921 cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag ccgttccacc
    25981 caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg cagctcatca
    26041 aagggagctt gctctgtggc atattccaaa taaagctcaa aactttgtcg ctgttctcta
    26101 gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa aagaattgct
    26161 gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt ttctagactg
    26221 tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc cataccaaat
    26281 gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg aagttgtctt
    26341 ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc cctgaataaa
    26401 tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc tccttgtgct
    26461 ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac aaaaagcgag
    26521 atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa tagtacatga
    26581 cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt gaccgggttt
    26641 cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc aactccccat
    26701 tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt tttcagatgc
    26761 tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt atattgcgat
    26821 agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa agcagctact
    26881 cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga aagcagcggt
    26941 gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca aaggcttttt
    27001 cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc ctgaataatt
    27061 tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc atttattaac
    27121 attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt ctccataatc
    27181 taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat ccttatttgg
    27241 ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat catcaaaaaa
    27301 caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac ggattgccat
    27361 gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca tgctttgatt
    27421 gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg gccgtttttc
    27481 gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaaccg ctgactcatc
    27541 attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag ataattggtt
    27601 agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca aactctcata
    27661 cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt tttttagttg
    27721 gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg ttgccagatt
    27781 agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg ttctgccata
    27841 gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat cagcccctgc
    27901 aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg caattttttg
    27961 caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga taaacaaaag
    28021 ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa ggagttggta
    28081 aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa aaacaatcat
    28141 attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact tgtacctgaa
    28201 cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga ttatgatgaa
    28261 gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt gatgccggaa
    28321 gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat tttattagaa
    28381 aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct aaacttgaaa
    28441 ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga aagattcatt
    28501 tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca tttatattat
    28561 ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg gtctgcgtta
    28621 gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc tccatgattt
    28681 ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc ttcttgataa
    28741 gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg aacagctgat
    28801 tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc ttttaaatgt
    28861 ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa tgaatggcca
    28921 ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt gtttccgccc
    28981 atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt ttttgctaaa
    29041 tcttgcgccc aaatactata actttctggc tctacgagag caccaggata aaaaataagt
    29101 aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc ttctttgttt
    29161 tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt ttgctgtata
    29221 tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat tagtattcgt
    29281 ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt cttaatcaag
    29341 tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc aaataaaaaa
    29401 agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat ttagcacata
    29461 aacgatacac agcttctgca taaatttcca ttgctttata catactactt aattcccatc
    29521 gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg aatgccacac
    29581 actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca gtcatgtctc
    29641 cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct agtggcacgt
    29701 ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct gctgttttag
    29761 ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata tctacaccga
    29821 tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc tcgccggatt
    29881 gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga actaattttc
    29941 ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc agacgagaaa
    30001 tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga acgacgatgc
    30061 cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt acttcagaaa
    30121 gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct ccagctttta
    30181 cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct ttttctgcat
    30241 aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct tctttttcgt
    30301 tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata aaacgaatac
    30361 gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt gccgcaatag
    30421 aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt atagttggat
    30481 cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc atatgacaca
    30541 agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag ccttctggat
    30601 ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc aaagcttcaa
    30661 tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt acggaaggaa
    30721 ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct gtcacaaatt
    30781 ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc ctccgacaca
    30841 acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca gaatgttctt
    30901 aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttatc cacgtgctta
    30961 tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg tatttgtcta
    31021 ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata atatcgcaat
    31081 taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg ctacagcacc
    31141 cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag attgataagc
    31201 agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca tgattcccat
    31261 tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga tatagaaaat
    31321 aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt cgccccaatg
    31381 taagatggtg tctgtgatta atccattgtt cataaccact tggataccac gagcaacagc
    31441 tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag agacaaattc
    31501 agactctttc atgccataag tgattgctac tacgatggac attactaaga ataacatagt
    31561 aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa ccgtacctag
    31621 taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat tcgcattcaa
    31681 ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga acaatacatt
    31741 aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa tatcgaaatg
    31801 cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt tttcgatctt
    31861 tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga acatcaagaa
    31921 tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta cgcctgttgc
    31981 aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata cgattgctac
    32041 agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag catagaatgg
    32101 aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca gcatcaatac
    32161 tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa tcccggcatc
    32221 tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa tgaagaaaga
    32281 gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac ctagcatccc
    32341 attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta ctcgttcata
    32401 tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg gtataaacca
    32461 agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg aaggcatttg
    32521 aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt tagagacaac
    32581 aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata ataacacgca
    32641 ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct tttttcaggt
    32701 ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac aaacattttc
    32761 gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt tatgcataaa
    32821 tcaaaaaaat actcaaaaca aacaaataa
//
LOCUS       DAZKSR010000023        31896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000023 DAZKSR010000000
VERSION     DAZKSR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.023"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACG8XG_000931"
     CDS             complement(26..601)
                     /locus_tag="ACG8XG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFQ0772864.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACG8XG_000932"
     CDS             complement(606..1580)
                     /locus_tag="ACG8XG_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0772865.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACG8XG_000933"
     CDS             1863..2831
                     /locus_tag="ACG8XG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0772866.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACG8XG_000934"
     CDS             2928..3221
                     /locus_tag="ACG8XG_000934"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772867.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACG8XG_000935"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACG8XG_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0772868.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACG8XG_000936"
     CDS             3678..4433
                     /locus_tag="ACG8XG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0772869.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8XG_000937"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8XG_000937"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0772870.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8XG_000938"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8XG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0772871.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8XG_000939"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8XG_000939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0772872.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8XG_000940"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8XG_000940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0772873.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8XG_000941"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8XG_000941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0772874.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACG8XG_000942"
     CDS             9170..9712
                     /locus_tag="ACG8XG_000942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0772875.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8XG_000943"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8XG_000943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0772876.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACG8XG_000944"
     CDS             11305..12207
                     /locus_tag="ACG8XG_000944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0772877.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8XG_000945"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8XG_000945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0772878.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACG8XG_000946"
     CDS             13650..14072
                     /locus_tag="ACG8XG_000946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0772879.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACG8XG_000947"
     CDS             14541..14771
                     /locus_tag="ACG8XG_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0772880.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACG8XG_000948"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACG8XG_000948"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0772881.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACG8XG_000949"
     CDS             16182..16358
                     /locus_tag="ACG8XG_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0772882.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8XG_000950"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8XG_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0772883.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACG8XG_000951"
     CDS             16853..17320
                     /locus_tag="ACG8XG_000951"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772884.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACG8XG_000952"
     CDS             17405..18289
                     /locus_tag="ACG8XG_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0772885.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACG8XG_000953"
     CDS             18403..18639
                     /locus_tag="ACG8XG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0772886.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACG8XG_000954"
     tRNA            18727..18807
                     /locus_tag="ACG8XG_000954"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACG8XG_000955"
     tRNA            18814..18885
                     /locus_tag="ACG8XG_000955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACG8XG_000956"
     CDS             19011..19181
                     /locus_tag="ACG8XG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772887.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACG8XG_000957"
     CDS             19401..20108
                     /locus_tag="ACG8XG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0772888.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACG8XG_000958"
     CDS             complement(20167..20811)
                     /locus_tag="ACG8XG_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0772889.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACG8XG_000959"
     CDS             20938..21225
                     /locus_tag="ACG8XG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772890.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACG8XG_000960"
     CDS             21333..21653
                     /locus_tag="ACG8XG_000960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0772891.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACG8XG_000961"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACG8XG_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23763
                     /locus_tag="ACG8XG_000962"
     CDS             23044..23763
                     /locus_tag="ACG8XG_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0772892.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="ACG8XG_000963"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="ACG8XG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0772893.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="ACG8XG_000964"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="ACG8XG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0772894.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="ACG8XG_000965"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="ACG8XG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0772895.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG8XG_000966"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG8XG_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0772896.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG8XG_000967"
     CDS             29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG8XG_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0772897.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="ACG8XG_000968"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="ACG8XG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0772898.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10607 a         5565 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DAZKSR010000024        31014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000024 DAZKSR010000000
VERSION     DAZKSR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.024"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..2480)
                     /gene="glyS"
                     /locus_tag="ACG8XG_000969"
     CDS             complement(405..2480)
                     /gene="glyS"
                     /locus_tag="ACG8XG_000969"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0772899.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="ACG8XG_000970"
     CDS             complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="ACG8XG_000970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0772900.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3783..4595)
                     /gene="recO"
                     /locus_tag="ACG8XG_000971"
     CDS             complement(3783..4595)
                     /gene="recO"
                     /locus_tag="ACG8XG_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0772901.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4738..5637)
                     /gene="era"
                     /locus_tag="ACG8XG_000972"
     CDS             complement(4738..5637)
                     /gene="era"
                     /locus_tag="ACG8XG_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0772902.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5652..6047)
                     /locus_tag="ACG8XG_000973"
     CDS             complement(5652..6047)
                     /locus_tag="ACG8XG_000973"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0772903.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="ACG8XG_000974"
     CDS             complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="ACG8XG_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0772904.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6524..8716)
                     /locus_tag="ACG8XG_000975"
     CDS             complement(6524..8716)
                     /locus_tag="ACG8XG_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0772905.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8740..9711)
                     /locus_tag="ACG8XG_000976"
     CDS             complement(8740..9711)
                     /locus_tag="ACG8XG_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0772906.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10417..11796
                     /gene="brnQ"
                     /locus_tag="ACG8XG_000977"
     CDS             10417..11796
                     /gene="brnQ"
                     /locus_tag="ACG8XG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0772907.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11952..12398)
                     /locus_tag="ACG8XG_000978"
     CDS             complement(11952..12398)
                     /locus_tag="ACG8XG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0772908.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12425..12601)
                     /gene="rpsU"
                     /locus_tag="ACG8XG_000979"
     CDS             complement(12425..12601)
                     /gene="rpsU"
                     /locus_tag="ACG8XG_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0772909.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12763..13191)
                     /locus_tag="ACG8XG_000980"
     CDS             complement(12763..13191)
                     /locus_tag="ACG8XG_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0772910.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13283..14143
                     /locus_tag="ACG8XG_000981"
     CDS             13283..14143
                     /locus_tag="ACG8XG_000981"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0772911.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14227..14577)
                     /locus_tag="ACG8XG_000982"
     CDS             complement(14227..14577)
                     /locus_tag="ACG8XG_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0772912.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14593..16320)
                     /locus_tag="ACG8XG_000983"
     CDS             complement(14593..16320)
                     /locus_tag="ACG8XG_000983"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0772913.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="ACG8XG_000984"
     CDS             complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="ACG8XG_000984"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0772914.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17604..19037)
                     /locus_tag="ACG8XG_000985"
     CDS             complement(17604..19037)
                     /locus_tag="ACG8XG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0772915.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19037..20440)
                     /locus_tag="ACG8XG_000986"
     CDS             complement(19037..20440)
                     /locus_tag="ACG8XG_000986"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0772916.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20670..21572
                     /locus_tag="ACG8XG_000987"
     CDS             20670..21572
                     /locus_tag="ACG8XG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0772917.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21613..23499)
                     /locus_tag="ACG8XG_000988"
     CDS             complement(21613..23499)
                     /locus_tag="ACG8XG_000988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0772918.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23596..24447)
                     /gene="licT"
                     /locus_tag="ACG8XG_000989"
     CDS             complement(23596..24447)
                     /gene="licT"
                     /locus_tag="ACG8XG_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0772919.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24905..26689)
                     /locus_tag="ACG8XG_000990"
     CDS             complement(24905..26689)
                     /locus_tag="ACG8XG_000990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0772920.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26689..28440)
                     /locus_tag="ACG8XG_000991"
     CDS             complement(26689..28440)
                     /locus_tag="ACG8XG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0772921.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28582..28806)
                     /locus_tag="ACG8XG_000992"
     CDS             complement(28582..28806)
                     /locus_tag="ACG8XG_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0772922.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29057..30643
                     /locus_tag="ACG8XG_000993"
     CDS             29057..30643
                     /locus_tag="ACG8XG_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0772923.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8675 a         6650 c         5522 g        10167 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcctatttt gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt
      421 tgatctaaac ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg
      481 ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc
      541 agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt
      601 tttttcattg cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca
      661 gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc
      721 aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct
      781 gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca
      841 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc
      901 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatca
      961 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
     1021 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc
     1081 ccaactgcaa agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct
     1141 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1201 gttgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt
     1261 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1321 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1381 atgacttgta cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1441 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1501 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1561 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc
     1621 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1681 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1741 acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca
     1801 tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca
     1861 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1921 tgtcctcgag aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca
     1981 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     2041 tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct
     2101 ttcgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct
     2161 ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc
     2221 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2281 ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa
     2341 aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2401 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2461 agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt
     2521 tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2581 acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc
     2641 gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg
     2701 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag
     2761 tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt
     2821 gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc
     2881 ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca
     2941 aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca
     3001 tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa
     3061 acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc
     3121 ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg
     3181 gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc
     3241 attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc
     3301 tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3361 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3421 ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga
     3481 ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc
     3541 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact
     3601 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3661 tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt
     3721 aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc
     3781 atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat
     3841 acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca
     3901 tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag
     3961 tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg
     4021 cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg
     4081 ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc
     4141 caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt
     4201 tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc
     4261 gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa
     4321 ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt
     4381 aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc
     4441 aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg
     4501 ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat
     4561 aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct
     4621 ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca
     4681 acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca
     4741 gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt
     4801 ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt
     4861 tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt
     4921 ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg
     4981 cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa
     5041 gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc
     5101 atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc
     5161 gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga
     5221 ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat
     5281 cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc
     5341 gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta
     5401 agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc
     5461 gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt
     5521 ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga
     5581 ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata
     5641 cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca
     5701 atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt
     5761 ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta
     5821 ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc
     5881 cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc
     5941 ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac
     6001 atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat
     6061 ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt
     6121 gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta
     6181 agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg
     6241 caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag
     6301 agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg
     6361 gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata
     6421 agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt
     6481 tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt
     6541 cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc
     6601 tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac
     6661 tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga
     6721 ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat
     6781 tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg
     6841 ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat
     6901 gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
     6961 ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
     7021 gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc
     7081 cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta
     7141 cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt
     7201 ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac
     7261 tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga
     7321 agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca
     7381 ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg
     7441 cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag
     7501 agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa
     7561 tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga
     7621 aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt
     7681 gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca
     7741 catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
     7801 tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag
     7861 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
     7921 ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
     7981 cagattgttt taattcttct gtctttttct cattcaatac atcattgact acaatgcctt
     8041 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct
     8101 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
     8161 gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt
     8221 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
     8281 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
     8341 tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat
     8401 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
     8461 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
     8521 tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt
     8581 ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
     8641 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
     8701 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
     8761 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
     8821 aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc
     8881 acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
     8941 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
     9001 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat
     9061 ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
     9121 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
     9181 gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
     9241 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
     9301 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
     9361 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
     9421 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt
     9481 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
     9541 ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
     9601 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
     9661 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
     9721 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
     9781 aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata
     9841 gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata
     9901 tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg
     9961 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    10021 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    10081 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    10141 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    10201 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    10261 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa
    10321 ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat
    10381 cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10441 tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat
    10501 ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta
    10561 ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca
    10621 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10681 ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac agctgctact
    10741 gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt
    10801 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg
    10861 gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt
    10921 gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa
    10981 acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg
    11041 cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa
    11101 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat
    11161 tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    11221 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta
    11281 ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11341 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11401 ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca
    11461 gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa
    11521 tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc
    11581 gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc
    11641 atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc
    11701 ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg
    11761 tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata
    11821 agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta
    11881 ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt
    11941 aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg gtttccatct
    12001 gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat
    12061 gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt
    12121 tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg
    12181 tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac
    12241 aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt
    12301 atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct
    12361 gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac
    12421 agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg
    12481 gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac
    12541 gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca
    12601 tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca
    12661 aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct
    12721 tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca
    12781 tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa
    12841 ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg
    12901 atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa
    12961 atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc
    13021 gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta
    13081 gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc
    13141 gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc
    13201 ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa
    13261 aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt
    13321 catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata
    13381 tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat
    13441 gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca
    13501 agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt
    13561 gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc
    13621 tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc
    13681 tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct
    13741 aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa
    13801 agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga
    13861 tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga
    13921 acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg
    13981 agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt
    14041 cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga
    14101 ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa
    14161 aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac
    14221 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga
    14281 atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc
    14341 tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat
    14401 tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc
    14461 actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa
    14521 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    14581 tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt
    14641 ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc
    14701 agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc
    14761 agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt
    14821 gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa
    14881 tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat
    14941 cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt
    15001 tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc
    15061 gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat
    15121 aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc
    15181 tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct
    15241 agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc
    15301 ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga
    15361 gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag
    15421 caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc
    15481 tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag
    15541 tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc
    15601 gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc
    15661 aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt
    15721 gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct
    15781 gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc
    15841 ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat
    15901 tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact
    15961 ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa
    16021 ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc
    16081 gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt
    16141 cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg
    16201 tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt
    16261 taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat
    16321 gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca cttattagat
    16381 atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg
    16441 ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga
    16501 acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc
    16561 aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt
    16621 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt
    16681 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca
    16741 gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg
    16801 ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg
    16861 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    16921 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    16981 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    17041 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    17101 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    17161 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    17221 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    17281 gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg
    17341 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    17401 gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata
    17461 tgtagattct cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc
    17521 tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac
    17581 aatagacttt tataaatcat tgatcaatca ttcgaaagat tttctccatt tgaatcaatt
    17641 acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg
    17701 ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat
    17761 actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc taattctact
    17821 gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca
    17881 taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt
    17941 ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc
    18001 caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca
    18061 gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaataata agaaaaggag
    18121 ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat tttgatctga
    18181 atgccatttc gttccatttc tttcaatgca taagcaggat attctcctcg tgactggaca
    18241 tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg
    18301 caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct
    18361 acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga
    18421 tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgcgcc catgaatggt
    18481 gcatgaagga tcatattgat ctcattgaat gtcaaccaat atttgacaag tcctttatag
    18541 cgattgaaga tgactcggca cagattttca taaaaaccaa cgagttctct ggaacgccac
    18601 gcaccatatt ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt cacaagcggc
    18661 tcgatcccgt acttttgaca ttctttaaag acatcctcat aaaatttcaa accagattca
    18721 tttggctctt tttcatcgcc cattggaaaa atacgactcc aagcaatgga catccgataa
    18781 gtcttgaacc ccatttctgc aaataaagca atatcttcct tataatgatg atacatatcg
    18841 atcgcaattt ttgctggata aaaatgattt tcatcaaatt caaacatttt cttttttcca
    18901 gtaatgacag gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc taacccacgt
    18961 ccgccttcat cataaccgcc ctcgcattga ttggctgctg tagctccgcc ccataaaaaa
    19021 tcttttctga atcccatcat acttcctcca ttacatgtgt attgcttgat aattctcttg
    19081 ccagttcatc ttggacttcg attccttctg cttttaaccg gctgattgtt tccttgaatt
    19141 gcggtaaata ctgctgatgt gcaataaaca actcattcat gatccgtttt gctgtctttc
    19201 ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc tgttccataa tcaccagtca
    19261 cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag
    19321 cagcaggaag ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca taggcagtga
    19381 cttctactac acaatctgcc ggcagatcag gaacagctcc atcattcttt gtagatacca
    19441 cgatctgtgt ttctttattt ccatagatcg aagcaatcgt ttcacaggca gcatctgaat
    19501 aataagcacc acctcgtttt tgcaattgtt caggtttatg atccaattct ggatctttgt
    19561 ataattcaaa cagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt
    19621 gatattcttc taaagcatgt tccagcattt cttcttcacg gtagtaatag cggtggtagc
    19681 cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat
    19741 ttttcggcaa tccattgtct tcatcgtata aacgatcaat caattctgcc gtgcggtcat
    19801 ttcctttatt atctgctact ttgtgccaat ggaagtgatt tagaccggca aacttgtaga
    19861 tcagctcttc ttttttcttg ccaagcattg ttggttctgt catcatcgcc attactggca
    19921 cattgcataa tcctatcact ttatcccatt tgccatatct gataactgct tctgtcacca
    19981 tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtctt
    20041 caacgattgc caaaaccact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat
    20101 ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat
    20161 ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc
    20221 gatccagagt tgcatagatt ttgacttcat acggagaagc atcccacata cgctgagaca
    20281 tctgactgac gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc
    20341 gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc
    20401 tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt
    20461 tacactttta ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt tctttccagt
    20521 aacatttaca aaagttagtg ttttctactg gatttttgta aatatcttgt caaaaaatgt
    20581 ctttattaag ctatataaca taaatccgtt tacaaatcat tttcaaaaaa gttataattt
    20641 acgtaaaaaa acaagaagaa ggtatctaca tgttattacg tgaaaaaatc aaagaaatct
    20701 ccttttctcc ttcagaaaat gaagtgattc agtttctctt aagcgattct ccagcacttg
    20761 gcgaaatgac catccaagag attgctgagc gatgttatgt ccacccgtcc acactcatcc
    20821 gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt tcgtaatgct tttttagctg
    20881 aaacagccta tcttgaaggg aattttcaag acgtagatgc gaatcttcct ttttccacca
    20941 cagacggtat tatgacgatc gcaaataaaa tggcttcttt agaacagatg acgattcaag
    21001 atacactttc tctcattcat catgaccaac tgcaaaaagc caaacaacta atgcttcaag
    21061 caaaaaaaat cgtgattttc gccagtaact cgaatacttt gatttctcaa gatttcatgc
    21121 tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac cacttttggt gaatcgttct
    21181 atgaagcgta caattgcacg agtgatatgt gcgggattct gatttcttat acgggtgaaa
    21241 acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa tcatgttcca attttagctt
    21301 tgaccagtat cggtgattca acgctatctt caatgagtac agccgctttg cgacttacga
    21361 cacgcgaacg actgtattcg aaaattggta attttacgat caatacatcg atttgttatc
    21421 ttctagacgt gttatatagt tgtgtttttg cagaaaatta tcaacagaat ttagtccatc
    21481 taatttctgt aggacaaaaa tttgataaac gccctatcag ctcagccatc atgaaagagc
    21541 ctttcaattt tgaaaataaa gaagtggact aaaaagaata attaagccta gtccattctt
    21601 tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc
    21661 ttgattttcc aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa
    21721 actgtatcct tcctcttcga ttttcttttt gtcgaagcgg agcaacagat cgccttgttt
    21781 cactctttct ccttgctgca ccaagctttc aaaatatttc ccttccaact gaacagtatc
    21841 caatccgaca tgaatcagca attccagacc tgcttcagaa accattccga ttgcatgttt
    21901 cgtcgggaac agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg
    21961 gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt
    22021 cagtggagcg atttgtcctt gtatcggtgt ttttacaatt tctttacctg atcttttctg
    22081 tctatcatct gtttcacctg cttcctcctt ccaaaagaaa aaagtcagcg caaaggatac
    22141 caccatcgca atggcgattc caatcaatac attaatcaca tccgttaaat caccatctgg
    22201 tctaatatag ttgggaattg taaagatgcc taatccacct ggattccaag aagttaaatt
    22261 cattaatcca ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt
    22321 tttacgtggc aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac
    22381 aatggctggt attgcgattg ctttgtcttt tgggtttcta agcttaaagt agagtgcagt
    22441 aagtgctgca gtttgtccaa aagacactac tgcaacaggt gtcatgatat tttgccaacc
    22501 attcacagaa aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac
    22561 tagaatttgc cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag
    22621 tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg aatgctggtc caacgagtaa
    22681 aaaacctaag ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat
    22741 ctcaggtact aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat
    22801 aatgggaatg acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg
    22861 ataaataggt gcttcaaaaa tagtgccaga aaatatggta gtcagtggtg cttctgctgc
    22921 accactcaaa gcttccagcg acaagctagg attcattaat gctgccccaa gcaacatccc
    22981 tacgaattca tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa
    23041 ttggaataca gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa
    23101 tgcgcctaag aagacaagta atgctgtcag tccacgaatc atcccaccag cagctagaat
    23161 agcaaggaat ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat
    23221 tttttctgtt gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa
    23281 cacgactggc acatgattcc cgatgacaac ttggtactgt cctcccgctc gcattaccgt
    23341 cacaacaccg tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt
    23401 gaaacgaagc cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt
    23461 ttctacaatt ttgctcgcta attcttgata ttttcccatt tctattccct ccaatttatt
    23521 agtggaaggt ttagccaact gaatgtcact acccttattt catgcgttct tgttaattta
    23581 tcctcctttt tttatttatt tacaacgatt ctcatgcact aaacgcgcaa tatggatcgt
    23641 taaatacatt ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc
    23701 tattttctca acgcaaccaa acgtatcctt atattttttt tgaataacct ggtacaattc
    23761 ctcagaaact tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg
    23821 taggtgtgtg acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta
    23881 atagcggacg atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg
    23941 ttggctttga atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc
    24001 tgataacttg atgtggtatt tatccgagat tttcttcact gtttttttcc cgatggcata
    24061 ctctttagga aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat
    24121 tttgtgacgt tcgatagccg tatacaaatg atccgtcaat gcaatataca ccgaatcatt
    24181 caactggact tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta
    24241 ctcaagagga atcgcttgtg ccaattcttg gaactgttgt gaaagtccgt catttgtcaa
    24301 ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc
    24361 caaacctttt cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt
    24421 gttattcagt actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta
    24481 tccaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt
    24541 ttagcttgca tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc
    24601 aagcactttt gtcaacgctt tcttagcggc tattgggcac ttctgactag ctgtcgtgat
    24661 tttttttaaa tctggtaatt acgacactaa cgctcacaga tgaagaatct gggaagctct
    24721 gttttatttt cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta
    24781 atatacggtg ttccagtacc agcttatcgg tgataagcca aatttatgca aaaatctgga
    24841 aagccttttt tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca
    24901 ctttttacac accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta
    24961 gaagtgtttc atgattccca cgttctacta tatgaccttt gtctaaaact aaaatcaatt
    25021 ctgcatcccg aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca
    25081 tttttgtcaa tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt
    25141 catccaatac tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc
    25201 gttgtccgct agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta
    25261 attcatcgat gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg
    25321 tgatagttgg attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat
    25381 cttgcaagac caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat
    25441 gatcatcaat caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga
    25501 tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga
    25561 caaaagagat attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa
    25621 actcgatctt acctgaagta atcgcttgat ctgctccagg attttgttgc ggtgcatatt
    25681 cttctgtatc catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc
    25741 tcaaaaagtc catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg
    25801 catagatcag tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg
    25861 ccaacgcaat cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaatttg
    25921 ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag
    25981 ctaaacgagc ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca
    26041 atttcgtgtt cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt
    26101 tctggtaata ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa
    26161 tttctccatt gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca
    26221 ataccatcaa aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag
    26281 aaacagtcga tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt
    26341 caaataatct tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc
    26401 attggaaaaa ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa
    26461 acaaaataac ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga
    26521 tgatccttgg caataggaca ttgatcacag acagggcaaa cgcaaataag atagcaacga
    26581 tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt
    26641 ctttaagtgg aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc
    26701 tccttctatc ttcgcttcta atttctgttt ctcccacatt cgttcatacc agccgcctaa
    26761 tgcaagcagc tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat
    26821 ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt
    26881 tcttatttct tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag
    26941 tgcatcatct aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct
    27001 ttgtttctgt ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt
    27061 tggtaattcc ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc
    27121 ttctgaaagt tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa
    27181 gtgatcttga ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat
    27241 cgaacatccg ccaaattgaa tttttccttg ataatcatcg tattcacgca tcagtaattt
    27301 gatgattgtc gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg
    27361 gatcgcaaat ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga
    27421 aatagctaca ttcagctcgc cttttgccgg agtctgcact gcacccagtt gttccacgat
    27481 atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa
    27541 tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat
    27601 aaatgagacc atctgcccga ttgagatcgt gccttccaag acatactttc cacctaaaat
    27661 aattgttgca acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc
    27721 taaaaaattg actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc
    27781 gagatcttct ttttcttgtc caaatgtttt aatgactttg atcccagtaa tactttcttg
    27841 tgtcttatca ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt
    27901 tgaccccagc actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt
    27961 taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga
    28021 atctgcaaag gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt
    28081 tgcatgagcc atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt
    28141 aaaatgtttg aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat
    28201 attcgtccgc cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa
    28261 gatccaccag gaaatgatct ttattgataa atttttatcg gcgatctcat caatgatgat
    28321 cccaatcact tttggaggag ctaactgtac caaggccact gcgaataggg agaaaatccc
    28381 aattaaataa tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat
    28441 tttactcctc ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg
    28501 aatagtctga atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc
    28561 aaaaagtctt tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg
    28621 gcgaattttc ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc
    28681 ctcattaacg ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa
    28741 gccacctact gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga
    28801 taccatataa atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag
    28861 tttattgaaa aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag
    28921 agaagaacaa aatttctttc attaaccagc aagacacgcc acgaaaaaaa attaggcata
    28981 ccaaatcttt ttctttaatt ttttctttct gaggtgtaga atgtaatcgt agataaatag
    29041 gaaaggtggt tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat
    29101 tgaacataca aatggtttat ctttagctga agtaaatgga acagtggatg tacccaaggg
    29161 aaaaagtttt tttaaaacac ttcttgctta ctctggacca ggtgcattag tagcagttgg
    29221 ttatatggat ccaggtaact ggtccacttc gatcactggt ggacaaaatt tccaatattt
    29281 attgatgtca gttattttga tgtctagtct gattgctatg cttttacaat atatggcagc
    29341 aaaattagga atcgtgaccc aaatggattt ggcccaagca atccgagcac ggacaagtaa
    29401 gacattgggt gtcattttat ggatcttgac tgaattagcc attatggcaa cagacatcgc
    29461 agaagtcatt ggtgcagcaa tcgccctcta cctgcttttt gatattccat tggtaattgc
    29521 cgtatttatc actgtgttag atgttttcgt ccttttattg ctgacaaaag tcgggtttcg
    29581 taaaatcgaa gctttggttg tctgcttgat tttcgtcatt ttattcgttt tcgtctatca
    29641 agttggcttg tctaaccctg attggcacag tttgtttgcc ggatttatcc ctaacagtca
    29701 cacattctca gactcaccgg tcatcggcgg cgaaactccg ctgactggag ctttagggat
    29761 tattggtgca acagtcatgc cgcataactt atatcttcat tcagcagttt ctcaaactcg
    29821 gaagatcgat caccaagacg aagaagatgt agcacaagcg gtacgctttt ctacttggga
    29881 ttctaatatc cagttgtcaa tggcttttct agtcaatgca ctgctattga tcatgggtgt
    29941 ggctgtattc aaaaccggtt ctgtccaaga tccttccttt tttggtctgt atgaagcttt
    30001 atctgatcca tcagctatga gcaatggtat cttgattgct gtagcaaaat ctggtatctt
    30061 gtctacttta tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac
    30121 gttgaccggt caagtcatca tggaaggctt catccatatg cgtatcccaa tttggctaag
    30181 aagactagtc acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag
    30241 tacaaaagga acgatcgaag aacataccgc tttgaataac ttgatgaatg aatcccaagt
    30301 ttttctggct tttgcattgc cattctcgat gattccttta ctgatgatga ccaatagcca
    30361 agcggagatg gggagccgat tcaaaaattc atttgttgta aaactacttg gctggttctc
    30421 tgtgattagt ctgacttact tgaatttacg aggattacca ggtcaaatcg aaagtttctt
    30481 cggcgagcaa gccacctcag ctcaactagc attggctgat caaatcgctt atgtaatcat
    30541 tgttcttgtc cttctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa
    30601 atatgaacag cgtttgcagg aaacgataga agaaatcgag tagaaactga ttcttttaca
    30661 caaaggaggt tgtgtcagaa gtgggcagct tcaagaaata agaaggaatt taagaaaatt
    30721 gcttttcaaa tttttgtgaa atttcggctt atttccgaag aagttacttc tggaacaccg
    30781 tgtatcagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    30841 cagcttttta tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgagcac
    30901 cgtttattcg gaaaaaggaa ctgcgacaag acttttgtca cagttccctt tcctacgatt
    30961 agtcaagtaa aaggtgtaag aaaaatagct cataaacgaa agcgacaaaa tcaa
//
LOCUS       DAZKSR010000025        30731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000025 DAZKSR010000000
VERSION     DAZKSR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.025"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            246..1115
                     /locus_tag="ACG8XG_000994"
     CDS             246..1115
                     /locus_tag="ACG8XG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0772924.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1324..2349
                     /locus_tag="ACG8XG_000995"
     CDS             1324..2349
                     /locus_tag="ACG8XG_000995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0772925.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2388..3338
                     /gene="manA"
                     /locus_tag="ACG8XG_000996"
     CDS             2388..3338
                     /gene="manA"
                     /locus_tag="ACG8XG_000996"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0772926.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3432..5135
                     /locus_tag="ACG8XG_000997"
     CDS             3432..5135
                     /locus_tag="ACG8XG_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0772927.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5137..7170
                     /locus_tag="ACG8XG_000998"
     CDS             5137..7170
                     /locus_tag="ACG8XG_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0772928.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7181..7621
                     /locus_tag="ACG8XG_000999"
     CDS             7181..7621
                     /locus_tag="ACG8XG_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0772929.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7821..8948)
                     /locus_tag="ACG8XG_001000"
     CDS             complement(7821..8948)
                     /locus_tag="ACG8XG_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0772930.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8951..9628)
                     /locus_tag="ACG8XG_001001"
     CDS             complement(8951..9628)
                     /locus_tag="ACG8XG_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0772931.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9892..11658
                     /locus_tag="ACG8XG_001002"
     CDS             9892..11658
                     /locus_tag="ACG8XG_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0772932.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11673..12110
                     /locus_tag="ACG8XG_001003"
     CDS             11673..12110
                     /locus_tag="ACG8XG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0772933.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12123..13274
                     /locus_tag="ACG8XG_001004"
     CDS             12123..13274
                     /locus_tag="ACG8XG_001004"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0772934.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13336..14034)
                     /locus_tag="ACG8XG_001005"
     CDS             complement(13336..14034)
                     /locus_tag="ACG8XG_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0772935.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14027..15379)
                     /locus_tag="ACG8XG_001006"
     CDS             complement(14027..15379)
                     /locus_tag="ACG8XG_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0772936.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15602..17002)
                     /locus_tag="ACG8XG_001007"
     CDS             complement(15602..17002)
                     /locus_tag="ACG8XG_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0772937.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17163..17900
                     /locus_tag="ACG8XG_001008"
     CDS             17163..17900
                     /locus_tag="ACG8XG_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0772938.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18025..18504
                     /locus_tag="ACG8XG_001009"
     CDS             18025..18504
                     /locus_tag="ACG8XG_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0772939.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18501..19268
                     /locus_tag="ACG8XG_001010"
     CDS             18501..19268
                     /locus_tag="ACG8XG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0772940.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19498..20067
                     /gene="lepB"
                     /locus_tag="ACG8XG_001011"
     CDS             19498..20067
                     /gene="lepB"
                     /locus_tag="ACG8XG_001011"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0772941.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20171..20773)
                     /locus_tag="ACG8XG_001012"
     CDS             complement(20171..20773)
                     /locus_tag="ACG8XG_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0772942.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20879..21109
                     /locus_tag="ACG8XG_001013"
     CDS             20879..21109
                     /locus_tag="ACG8XG_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772943.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21100..21348
                     /locus_tag="ACG8XG_001014"
     CDS             21100..21348
                     /locus_tag="ACG8XG_001014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772944.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21454..22038
                     /locus_tag="ACG8XG_001015"
     CDS             21454..22038
                     /locus_tag="ACG8XG_001015"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0772945.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22041..23114
                     /gene="prfA"
                     /locus_tag="ACG8XG_001016"
     CDS             22041..23114
                     /gene="prfA"
                     /locus_tag="ACG8XG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0772946.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23107..23946
                     /gene="prmC"
                     /locus_tag="ACG8XG_001017"
     CDS             23107..23946
                     /gene="prmC"
                     /locus_tag="ACG8XG_001017"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0772947.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23939..24118
                     /locus_tag="ACG8XG_001018"
     CDS             23939..24118
                     /locus_tag="ACG8XG_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772948.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24140..25165
                     /locus_tag="ACG8XG_001019"
     CDS             24140..25165
                     /locus_tag="ACG8XG_001019"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0772949.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25356..26600
                     /gene="glyA"
                     /locus_tag="ACG8XG_001020"
     CDS             25356..26600
                     /gene="glyA"
                     /locus_tag="ACG8XG_001020"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0772950.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26753..27700
                     /locus_tag="ACG8XG_001021"
     CDS             26753..27700
                     /locus_tag="ACG8XG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0772951.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27834..28463
                     /gene="upp"
                     /locus_tag="ACG8XG_001022"
     CDS             27834..28463
                     /gene="upp"
                     /locus_tag="ACG8XG_001022"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0772952.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28602..28844)
                     /locus_tag="ACG8XG_001023"
     CDS             complement(28602..28844)
                     /locus_tag="ACG8XG_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772953.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28965..29037
                     /locus_tag="ACG8XG_001024"
     tRNA            28965..29037
                     /locus_tag="ACG8XG_001024"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28998..29000,aa:Thr,seq:cgt)
     gene            complement(29156..29794)
                     /locus_tag="ACG8XG_001025"
     CDS             complement(29156..29794)
                     /locus_tag="ACG8XG_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0772954.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29917..30549)
                     /locus_tag="ACG8XG_001026"
     CDS             complement(29917..30549)
                     /locus_tag="ACG8XG_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0772955.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10463 a         5401 c         6199 g         8668 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt
       61 cttattactt gatgcagaac agctttttca caacctcttt ttataagagt ttttactcat
      121 taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac aaaatcaaag
      181 ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag aggggagcaa
      241 gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta gccattcggg
      301 tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt gatttgaaaa
      361 gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa atgttgctgc
      421 tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg aaccgttttt
      481 cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc cgtaaaatga
      541 attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg ctcgtagacc
      601 cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa agagaaattc
      661 cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc agtaaactat
      721 caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca aaagaacgcc
      781 aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc atccatgtat
      841 tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca attgatttgc
      901 ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact gaaagcatgg
      961 aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac aaaaagcaca
     1021 cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt gagccaaaag
     1081 aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa cgattgtcgc
     1141 atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag aaagagaaaa
     1201 tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa aaaaggttga
     1261 ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa gcaggagaaa
     1321 aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg attttatcca
     1381 ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc cggctttgcc
     1441 caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc agcctcactg
     1501 acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg aaagatagaa
     1561 ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat cactttgcgt
     1621 catttactga cacatacttc tgatatcaaa acatggatac caaacagaga taagctatct
     1681 caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact aggaaaagaa
     1741 gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga aatttaccaa
     1801 gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat gatgaacagc
     1861 ttatttcttc cttttccttc caaaaaaatc gttcccacag aacaactgcc ttcgggtgag
     1921 gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca tgcaggtaac
     1981 gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta tttgaacaag
     2041 ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt actgcaagat
     2101 caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa ggatgtagaa
     2161 gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt gattgatata
     2221 aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga tcatcgtgcg
     2281 gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa agcggtatta
     2341 ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg caggaaccta
     2401 tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta cgttcagtct
     2461 ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt gcgcatcctc
     2521 atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat gaactatggt
     2581 cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta ttgatcaaga
     2641 tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc tatgggatga
     2701 aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct gaacctggtg
     2761 cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag atgatcaaag
     2821 atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat ttcttttatg
     2881 taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa acacagcaaa
     2941 gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa ggacaaacaa
     3001 gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa acaccagaat
     3061 tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta gaaactgagt
     3121 ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa caagcccctt
     3181 atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga aaaacatatc
     3241 cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg gcggtccaag
     3301 gtgaactagc aatcattgcg tctgaaccag gcaaatagtt cccagatagc tgaaagcagc
     3361 agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata aatagaagtc
     3421 tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg tctatgaaac
     3481 aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta agtctgttta
     3541 tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg cacaccacat
     3601 tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc agttattcag
     3661 gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct tctttagggt
     3721 tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg ccgcttgccg
     3781 gcatcgtata tgaacaattt gtttctagat tccatgtgaa attcaaggct ggatatgaaa
     3841 tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata attggaatcg
     3901 tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt gtgtcttggc
     3961 tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta aaagtactat
     4021 ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa ttctcacaaa
     4081 cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt ttagggatct
     4141 tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt ggagcattga
     4201 tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg ctgcgaccgt
     4261 atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt caatggttag
     4321 atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt gccaatgcgc
     4381 cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt gtgaccttca
     4441 tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa acggtcaaag
     4501 aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct tgcgactcag
     4561 gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca gcagaaaaaa
     4621 tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta aatcagctgg
     4681 ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa agtcaaatct
     4741 atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa gaagcgatcc
     4801 aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa aaaacgtcgc
     4861 ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact atgggcatga
     4921 cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca aaagaatcaa
     4981 tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa ctagctgtaa
     5041 ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg gtagctacga
     5101 atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc cagagaaaaa
     5161 caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa aatgatgaat
     5221 ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga agtctcattg
     5281 aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat tgaggcatca
     5341 gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga cttatccact
     5401 gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc gttgagtctt
     5461 gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga tatcaaacag
     5521 ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg atacgagatc
     5581 gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt agaaatcaat
     5641 gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt ttttcgattc
     5701 gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt gatccaaaaa
     5761 aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac attgacgatg
     5821 gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct ggcaaacaaa
     5881 gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac aaaacaactt
     5941 tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac gaactcattt
     6001 gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt aaaacaactg
     6061 attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac tttgtacaaa
     6121 atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga agagacactg
     6181 accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa tgcaatccgt
     6241 aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat cgcttatatg
     6301 gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat cagtattgca
     6361 ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt gaaaaggtat
     6421 tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa aatcgattta
     6481 caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg tggaaaatat
     6541 attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga agctttttcg
     6601 catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga gtggatgata
     6661 gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt actcggtcgt
     6721 ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg cagcttattt
     6781 ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg gctgcttaag
     6841 cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt ccatacatcc
     6901 agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc gttgaccatt
     6961 gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct ggcgccttcg
     7021 ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat catcatgaat
     7081 gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca gttgttagcg
     7141 aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc taccagcaga
     7201 aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg agatcggcca
     7261 gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga tcgaaagaca
     7321 agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga ctgacaaaga
     7381 tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg tggactttgg
     7441 aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa aaaaagaaca
     7501 tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc aagtaatggc
     7561 gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg gacatatata
     7621 accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa ccaagataaa
     7681 aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag cttttgaaaa
     7741 ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt ttttgtcacc
     7801 gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc catacctatg
     7861 aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa agtaacctgt
     7921 tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa atcaacataa
     7981 gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa caagcgatac
     8041 tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg ttcaaataaa
     8101 gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag gaacagaaag
     8161 ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat gatcgttcct
     8221 acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga gacgatcaaa
     8281 aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg gatgctatgt
     8341 ttctttacca tactatccag taatagattg actagagaga ttacctgctt tcttttttca
     8401 taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa actagaaatc
     8461 attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg caagtcaaaa
     8521 atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac acgtttttgt
     8581 tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag tatatctttt
     8641 tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt tgcatgtaag
     8701 attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt ctcattctca
     8761 tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt cagttcatcg
     8821 aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag cacgaccaac
     8881 aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc aaatgtaagt
     8941 aaagacatag ttacacctca tataatttac gataaatagc taaaatctct tccatatttg
     9001 aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa atcgattgga
     9061 tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc cattgtctga
     9121 tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt tcatcgaata
     9181 acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt ttcattccta
     9241 gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt tggaaaacag
     9301 tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa tcaacagccg
     9361 atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg cgataatctg
     9421 tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt ggcagcagtt
     9481 gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc aaagcagcta
     9541 cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta ccacgtttca
     9601 tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat ttaataacat
     9661 aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc atattataaa
     9721 aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa tctttttatt
     9781 ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac tatttctctt
     9841 tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa aatggaacgg
     9901 acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg cagtacttta
     9961 tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt aacggctcta
    10021 tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag cccaatgcta
    10081 acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc tggtgaccgc
    10141 ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga tattccaatg
    10201 atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa atttgatcag
    10261 ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt ttcagcaggt
    10321 cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt tgtcgatgca
    10381 ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt gattccattg
    10441 acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat caatcatggg
    10501 attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat tctattcttg
    10561 cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt gtttggcaaa
    10621 ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg cgggatccat
    10681 gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt tataggcgga
    10741 ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc gccagcttct
    10801 ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct tcctgttatt
    10861 ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat tttgaaagcg
    10921 gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa ggcagctaag
    10981 caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg cgtacaaaaa
    11041 atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc gatcttgaga
    11101 aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat cagccaactg
    11161 acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag agcaaaacaa
    11221 aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc tcgatatgat
    11281 gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat tgagaatgag
    11341 ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct acttgtccac
    11401 gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat attagacaag
    11461 aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga tgaacctgat
    11521 tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc acctcaatcc
    11581 attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat tgcacaatat
    11641 ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc gacatgatcg
    11701 agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt cagatgcttg
    11761 tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga gacaaaatgt
    11821 tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa gcaaaaaaat
    11881 atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat tttggtaccg
    11941 aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga gatgagcatt
    12001 tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt gtccagctag
    12061 cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa ggagagagaa
    12121 aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt ggagaggttt
    12181 tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc attgatgctt
    12241 taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa aaaatcactg
    12301 taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc caagaaatcg
    12361 ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt attgctgagt
    12421 tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct ttagatatca
    12481 ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg tataaatatc
    12541 ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca gtcgatcgaa
    12601 tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct ttcaaagaat
    12661 gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg gttttatatg
    12721 tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact ggtcatgcga
    12781 ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca atgcaagatg
    12841 cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg ctgattgcca
    12901 aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt catcgcttcc
    12961 aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta cgtaagttag
    13021 gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac ttgacttgcc
    13081 cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa gatgaacaaa
    13141 gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt caagaagtca
    13201 caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac cgttatgcca
    13261 agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt tgtcacattc
    13321 ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca attctttacc
    13381 gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat ttcttgcaag
    13441 gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt cagattggtc
    13501 ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac cttcacctaa
    13561 gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt cttcgttatg
    13621 gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt gatccagtgc
    13681 actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta gcaattggta
    13741 gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca aagaatcttt
    13801 ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac cgccaaagaa
    13861 aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct ccgtatggca
    13921 agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt tatcaccata
    13981 tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag ccacggttca
    14041 ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat acagcagtgt
    14101 aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg attacttctg
    14161 gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt agacgtaaag
    14221 tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata tcggcaaagt
    14281 tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa ttggcattta
    14341 ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt acttgattga
    14401 tacctacagg atttttgaca agtactaatg tacatttttt cccattgata ttgatgactt
    14461 cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta tcaggatcga
    14521 cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta tacataccgc
    14581 caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca ttgtccatcc
    14641 gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa tttggacagt
    14701 aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga tgacaatttg
    14761 gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct tcatggtcaa
    14821 agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt gaatcaccgt
    14881 tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt ttataggtcg
    14941 tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat aagaacagtt
    15001 ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct aggacagcaa
    15061 actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt ccctgtacca
    15121 tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa atattgactg
    15181 ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact tggtagtctt
    15241 ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct ggtaaacttg
    15301 atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact gcaatcgcaa
    15361 aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga ataaattcaa
    15421 ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt aagaaaaggt
    15481 tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa aacagcttga
    15541 aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct atattcccat
    15601 tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt gtttcttttg
    15661 gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc agtgtagcaa
    15721 tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa ccagttgttc
    15781 caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct gtagcagata
    15841 aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg actgattttc
    15901 gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca cttttgagga
    15961 aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg ccgcctacag
    16021 aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt gtgatttgaa
    16081 aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa taaaacatcg
    16141 aattgatcca tttgacactc atattggaat cttggaataa atggtccttt tccaatagat
    16201 aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct aatttggtaa
    16261 acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc cattcccggc
    16321 actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg atcaggatca
    16381 agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca aacccagagt
    16441 tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc caacgatcaa
    16501 aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat tgaaaccaga
    16561 ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc ggttgattca
    16621 tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa aaaatgacaa
    16681 aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt tccagcagta
    16741 taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca gtcacactca
    16801 ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct ggttcgcgaa
    16861 aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa taaaaaacga
    16921 tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc tgcagccttc
    16981 gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt ctaccagtat
    17041 acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac agctttcgat
    17101 taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg aggaataaca
    17161 aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata aattatgata
    17221 cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct aagatattaa
    17281 tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct caatatgcag
    17341 agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat ttgcgaagat
    17401 tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat catgttgcgc
    17461 taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca aaccatcttg
    17521 aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga ttagatcttg
    17581 ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg accatctcgc
    17641 caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag atttacggga
    17701 cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct ttgcaaatgt
    17761 gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct gccaagcagc
    17821 aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa acatcaaccg
    17881 tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa ataaagaatt
    17941 tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga aacaagaata
    18001 aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa gccaatcgct
    18061 gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag ttccagctac
    18121 cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac ttcagtctcg
    18181 acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag tacatgggac
    18241 gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc aagcgaaaca
    18301 agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac aagtccggat
    18361 acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa cacgcaaaat
    18421 gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca aacgtataac
    18481 cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg gtgcataaat
    18541 tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg attttaagtg
    18601 tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac caaaactatc
    18661 ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact gttttaaatg
    18721 tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt tttgtgaagt
    18781 atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat ggaaattcga
    18841 tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat caattgaaat
    18901 tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat gaactcggtc
    18961 gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat gtcaagaaaa
    19021 caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg gaaaaagtag
    19081 ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg ttcatcgtcg
    19141 gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg aaattgattg
    19201 caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa agaagaagac
    19261 attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt tattcggaat
    19321 aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga aagataagac
    19381 gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag aaagtacaac
    19441 gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag gtaagctgtg
    19501 aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat tttattagct
    19561 agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc tacattaagt
    19621 gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga tattattact
    19681 acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg tatgcctgga
    19741 gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta tgatgaacct
    19801 tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga atatgcttat
    19861 agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga ctttgaatat
    19921 actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc aaaagacagc
    19981 cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt ccgttattgg
    20041 cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg acatgttttt
    20101 tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc actttttttg
    20161 caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt ttgttcataa
    20221 atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc gttttttcca
    20281 atcgattatt caattcttct tggctaaagc cattatggac tttcggatga ctaatttgtt
    20341 cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt tcataaagtt
    20401 ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga aggatgagat
    20461 tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg acatttttca
    20521 gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa gcatcggcaa
    20581 tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt ccgcctccgt
    20641 aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt tcttcgcgaa
    20701 taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg gaatcaaaaa
    20761 tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact tattatagca
    20821 cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta gaaagaatat
    20881 gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag aaatattact
    20941 agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg aggaatcaag
    21001 taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta tcactctttc
    21061 acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa aaaagactat
    21121 catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc agcaaaacat
    21181 tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata tgatcaaact
    21241 atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa tttatgggat
    21301 gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc aatagattta
    21361 atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat caagtataat
    21421 accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt taagtatggc
    21481 gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta tgaagaacaa
    21541 aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt aggcgttgtt
    21601 tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac agacgtattt
    21661 gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga agcgcaattc
    21721 ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga tattccggtt
    21781 atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc aaaatattta
    21841 ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg ccacaaaaaa
    21901 gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga tcaagtgcaa
    21961 attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca cccatcaatg
    22021 attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac cgttatgaag
    22081 agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt tttatgcagc
    22141 tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt tataaagaag
    22201 tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat gatgaaatgg
    22261 ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc ttagaagaaa
    22321 aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc atcatggaaa
    22381 tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt ttcaatatgt
    22441 accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct aacgttaccg
    22501 gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta tattcgaaat
    22561 taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa tcgcaagggc
    22621 gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa gtagagatcg
    22681 atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct ggcggacagc
    22741 acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg atcgttgttg
    22801 ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa atcttgcgtg
    22861 ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc aatcgaaaat
    22921 cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc ccgcaaaatc
    22981 gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt ttagcaggga
    23041 aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa ctggaagcta
    23101 tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc ttttttagag
    23161 gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa acaatggaaa
    23221 aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca gcggttgatc
    23281 gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct aggatatgca
    23341 gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg accagaaaca
    23401 gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga agtgatagat
    23461 attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa aaattggcat
    23521 atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa tgctcagaaa
    23581 gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat ggatcagcaa
    23641 tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga actgatggat
    23701 gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga tggattagct
    23761 atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg aaagattttt
    23821 ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca agcatttcca
    23881 gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat ccaagtttat
    23941 ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa aacacttatt
    24001 tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat tgacaaatag
    24061 attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga atgtatgaaa
    24121 aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat tgcaagaggc
    24181 agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag tatacggatt
    24241 gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa aagggagacc
    24301 gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag agtatgtgga
    24361 caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac cattaacttt
    24421 gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat tatccacagc
    24481 tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt ccggtgttcc
    24541 gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg cagatcatgt
    24601 atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta cgcgaattgg
    24661 tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga ttttacgacc
    24721 aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg ctattgatca
    24781 gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca aacactactc
    24841 acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag ttcaatgggc
    24901 aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg atcaagtgcg
    24961 gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg cggctaaagg
    25021 attgtttgca ggtctaagag gattagatga accaacactt ggattagatt tgatttttgt
    25081 acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga aaaaagcagc
    25141 aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt tagtatgata
    25201 caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac taatcgtaat
    25261 agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat tgtaggaaaa
    25321 taaaattgta ggaaaataaa aaggagctgt tgtttgtggt agattacaaa acgtttgacc
    25381 ctgatctttg ggctgcgatc gccaaagaag aagaaagaca agaacataat ttggagctga
    25441 ttgcctctga aaactttgtc tccgaagcag tgatggctgc acaaggaagt atcttgacaa
    25501 ataaatacgc agaaggttat ccagggcatc gctattatgg tgggtgtgaa tttgtcgata
    25561 tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt cggagcgaaa tttgcgaacg
    25621 tccagcctca ctccggctct caagccaaca cggctgctta tctcgctttg gtagaaccag
    25681 gggacacgat cttaggaatg gatctttccg caggcggaca tctaacacat ggatcaccag
    25741 tcaacttcag tggcaaaacc tatcactttg ttgcttacgg tgttgatccg acaacagaag
    25801 ttattgatta taatgtggta cgtattttag ctagaaaaca tcaaccaaaa ttgatcgtag
    25861 caggtgccag tgcttacgga cgaacgatcg actttgcgaa attcagagaa atcgcagatg
    25921 aagtaggcgc aaaactgatg gtagatatgg cacatatcgc aggattagta gcagcaggac
    25981 ttcatcctaa tccagttcct tatgcagata tcacaacgac taccactcat aaaacattgc
    26041 gtggccctcg aggcggcatg attttgacaa acgatgaagc attagcaaaa aaaataaata
    26101 gtgcggtatt tcctggtatt caaggcggcc ctctagagca tgtgattgcg ggtaaagcag
    26161 tagcattcaa agaagcatta gatcctgcat ttaaagaata tagtgaacaa atcatcgcca
    26221 atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg aaccagagtc atttcagggg
    26281 cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt aggtataaat ggaaaagaag
    26341 cagagagcat tttggacagt gtgaatatca ctgtaaataa aaactcgatt ccttttgaaa
    26401 cactaagtcc attcaaaaca agcggtattc gaatcggcac gccagcaatc actactcgcg
    26461 ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt tgtcaaagca ttacaagcaa
    26521 aagatgataa cgctcaacta gacgaagtca aaacaggtgt acgcgaatta actgaaaaat
    26581 ttccgttgca taaaaaataa aaatttaaat tccattagta aacagaaaag ttcaatgttg
    26641 ggcttttctg tttatttttt gggaaaacgg attctttgac aatatcggtg atgctctcta
    26701 gtatatgagg tgtaatttta gtcttcacac taaatctcag ggaggaaaag tcatgactga
    26761 tcgtatctcg ttagaaaagg aagcagtagc attcagtgat gcaaatgcgc ctcatccgcg
    26821 tatctatgaa ctagcaccgg aagaaggaag agttttacta gaagaagtac aagaatcacc
    26881 tgtgaacaaa tattccgtcg atattgaaga cttgatggtg gatactggcg aatggggaaa
    26941 actcaatatt cgtctcgtac gaccagaagg gaatatggat aaattaccag ttatttttta
    27001 tattcatgga gcaggctggg tttttggcag tgctcaaacc catgataaat tgatcagaga
    27061 acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa tatagtcgat caccagaagc
    27121 aaaatatcca acagcaattg aacaaaatta tgctgtgttg caacagctga ataaattagc
    27181 caagtcaaaa aatctggatc tcgagaatct aatcgttgca ggagattcag taggtggtaa
    27241 catggctaca gtcatgacga tcttaacaaa agaacgaaag ggcctcccaa tatccaagca
    27301 attgctgtat tatccagtca caaacgctga ttttgataca gattcctatc atgaattcgg
    27361 agaaaactat tttttgacaa gagaaggaat gatgtggttt tgggaccaat atacgacgaa
    27421 tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga gcaaatataa atgaactaaa
    27481 tgatcttcct tcagcaatga ttttaacggg agaagcggat gtattaagag atgaaggtga
    27541 ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt acacaagtac gcttccaagg
    27601 aatcatccat gattttgtca tggtaaatgc tatggatcag acaaaggcaa cgcgtgcagc
    27661 aatggcatta tctacttctt ggatcaagaa aaaagaataa gctttgcttg tccgcaggaa
    27721 aacttctttt caaaaacgga ttttcttagg aaaaatggcg agtttgacta gtcaatcact
    27781 tgattttgtt ttacaattaa aaagaaacga cagaacaaag gagagcttaa aaaatgggca
    27841 aatttcaagt gatcgatcat ccattgattc aacacaaact aactattatt cgggacaaaa
    27901 actgcggaac aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg atggcatatg
    27961 aagtatcacg ggatatgcca ttggaagaca tcgtgatcga aacaccgatc actgaaacaa
    28021 cacaaaaaac gttgtctggt aaaaaagtag cgattattcc aatcttgcgc gcaggtattg
    28081 gaatggttga cggtatcttg gaattgattc cagctgctaa agtgggacat atcggtcttt
    28141 atcgagatga agaaacattg gaaccacatg aatatttcgt caaacttcca gaagacattg
    28201 atgcacgtca gttgtttgta gtagatccaa tgttggcaac aggcggatca gcaatcatgg
    28261 caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt ttggtagcag
    28321 cacctgaggg agtaaaagct ttgcaagaag cgcatcctga tattgacatc tatacagctt
    28381 ctctagatga aaaattgaat gaacatggtt atatcgttcc tggactaggg gatgcgggtg
    28441 accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa cttttgtcac
    28501 atcctttttt ggtcattttt aacgtttgat gcagaacagc tttttcacag ctttcgtttg
    28561 catataaaaa aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt ttttagttct
    28621 ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt ttctgcagtt
    28681 tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc agtttgcgac
    28741 ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc aatttccgat
    28801 ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc tgcctttcac
    28861 atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt ccatcggact
    28921 ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc ttagctcagt
    28981 tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata gctggcatta
    29041 aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc tttttgttta
    29101 aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata aaaatttagg
    29161 agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact gcccaacttg
    29221 taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc accccttgta
    29281 tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta ctgtaatgga
    29341 taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac ttactagaaa
    29401 aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata ggcaaaataa
    29461 taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc catttcaaaa
    29521 tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa aaatatttgg
    29581 ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct tttttaccaa
    29641 aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta ataatggatg
    29701 gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat atcccttttt
    29761 taagttctat tttttttcct aaaatgttat tcatgataat aactcctact attcagtttt
    29821 tgaagataga gtcagtatac atgaagagtt aaaataggca atgttttttt atggatattt
    29881 gtattgctaa aaatttatct tatatattca ttttgattat actactttta tttgtaaaaa
    29941 ttttctagca aaaccaagtg cttgcaactg attaagcgta caatttttta gatgttccga
    30001 gcgtacttca atcatttcaa aggagttgct acttaaatcg atatttttta aatcagtatc
    30061 aaaaaagcta gcattttcta aatcattagt agaaaacatt attttagtga gggaacagtc
    30121 tacaaaatct gcgcttgata agacagagtg ctcaaaggta ctttctttta tgtttgaacc
    30181 agataaattt acatagtcca ttttacaatt ctcgaataac gtgttcccaa tataactttc
    30241 catcaattta gtacctgtta atttacaatt gacaaatttc acgcgatgca acatgctttt
    30301 tgtcaatata cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa tctccattct
    30361 ttgaaaatca ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa taattggatg
    30421 gcagattatt tggttttcga tgacctcttt ttgtgaaagg atattataca gttcaaaatc
    30481 ttcgttttca tagatatgaa aaaaggatcc tttattcaag cttgcttcat taactttagg
    30541 aaaaatcata tttaattacc tccattttca taaagtcata ttaacaaaag ctaaaccaac
    30601 ttacattagc agaaaatgag ataggttttc gcctttttcg ataacgatag tgtcgctatc
    30661 tcttgaaacg ttcttagaat tgccctgaga gacacaaatt aggcgtaatt ctaataaaaa
    30721 tcttagaata a
//
LOCUS       DAZKSR010000026        30591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000026 DAZKSR010000000
VERSION     DAZKSR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.026"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..731
                     /locus_tag="ACG8XG_001027"
     CDS             363..731
                     /locus_tag="ACG8XG_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0772956.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="ACG8XG_001028"
     CDS             911..1852
                     /locus_tag="ACG8XG_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0772957.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="ACG8XG_001029"
     CDS             1921..2652
                     /locus_tag="ACG8XG_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0772958.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="ACG8XG_001030"
     CDS             2796..4976
                     /locus_tag="ACG8XG_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0772959.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="ACG8XG_001031"
     CDS             4996..5478
                     /locus_tag="ACG8XG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0772960.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="ACG8XG_001032"
     CDS             5494..6255
                     /locus_tag="ACG8XG_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0772961.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /gene="murC"
                     /locus_tag="ACG8XG_001033"
     CDS             6386..7720
                     /gene="murC"
                     /locus_tag="ACG8XG_001033"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0772962.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="ACG8XG_001034"
     CDS             8027..8512
                     /locus_tag="ACG8XG_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0772963.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="ACG8XG_001035"
     CDS             8505..9188
                     /locus_tag="ACG8XG_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0772964.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="ACG8XG_001036"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="ACG8XG_001036"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0772965.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8XG_001037"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8XG_001037"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0772966.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8XG_001038"
     CDS             12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8XG_001038"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0772967.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="ACG8XG_001039"
     CDS             13485..13817
                     /locus_tag="ACG8XG_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0772968.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8XG_001040"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8XG_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0772969.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="ACG8XG_001041"
     CDS             14643..16016
                     /locus_tag="ACG8XG_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0772970.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8XG_001042"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8XG_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0772971.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="ACG8XG_001043"
     CDS             17071..17511
                     /locus_tag="ACG8XG_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0772972.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="ACG8XG_001044"
     CDS             17655..18356
                     /locus_tag="ACG8XG_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0772973.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="ACG8XG_001045"
     CDS             18353..19474
                     /locus_tag="ACG8XG_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0772974.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8XG_001046"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8XG_001046"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0772975.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="ACG8XG_001047"
     CDS             20853..21761
                     /locus_tag="ACG8XG_001047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0772976.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="ACG8XG_001048"
     CDS             21796..22068
                     /locus_tag="ACG8XG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0772977.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="ACG8XG_001049"
     CDS             22079..23125
                     /locus_tag="ACG8XG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0772978.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="ACG8XG_001050"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="ACG8XG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0772979.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26822)
                     /locus_tag="ACG8XG_001051"
     CDS             complement(26136..26822)
                     /locus_tag="ACG8XG_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0772980.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26800..27294)
                     /locus_tag="ACG8XG_001052"
     CDS             complement(26800..27294)
                     /locus_tag="ACG8XG_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0772981.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="ACG8XG_001053"
     CDS             complement(27314..28534)
                     /locus_tag="ACG8XG_001053"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0772982.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="ACG8XG_001054"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="ACG8XG_001054"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0772983.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10251 a         5446 c         6594 g         8300 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg t
//
LOCUS       DAZKSR010000027        29240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000027 DAZKSR010000000
VERSION     DAZKSR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.027"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..1594)
                     /locus_tag="ACG8XG_001055"
     CDS             complement(170..1594)
                     /locus_tag="ACG8XG_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0772984.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            1805..2251
                     /gene="nrdI"
                     /locus_tag="ACG8XG_001056"
     CDS             1805..2251
                     /gene="nrdI"
                     /locus_tag="ACG8XG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0772985.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(2316..4298)
                     /locus_tag="ACG8XG_001057"
     CDS             complement(2316..4298)
                     /locus_tag="ACG8XG_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0772986.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4312..5208)
                     /gene="murQ"
                     /locus_tag="ACG8XG_001058"
     CDS             complement(4312..5208)
                     /gene="murQ"
                     /locus_tag="ACG8XG_001058"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0772987.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(5211..6272)
                     /locus_tag="ACG8XG_001059"
     CDS             complement(5211..6272)
                     /locus_tag="ACG8XG_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0772988.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(6483..7010)
                     /locus_tag="ACG8XG_001060"
     CDS             complement(6483..7010)
                     /locus_tag="ACG8XG_001060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0772989.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(7077..8579)
                     /gene="eat(A)"
                     /locus_tag="ACG8XG_001061"
     CDS             complement(7077..8579)
                     /gene="eat(A)"
                     /locus_tag="ACG8XG_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0772990.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(8837..9958)
                     /locus_tag="ACG8XG_001062"
     CDS             complement(8837..9958)
                     /locus_tag="ACG8XG_001062"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0772991.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            10084..11025
                     /locus_tag="ACG8XG_001063"
     CDS             10084..11025
                     /locus_tag="ACG8XG_001063"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0772992.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(11175..12071)
                     /locus_tag="ACG8XG_001064"
     CDS             complement(11175..12071)
                     /locus_tag="ACG8XG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0772993.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            12340..13680
                     /locus_tag="ACG8XG_001065"
     CDS             12340..13680
                     /locus_tag="ACG8XG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0772994.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            13898..14341
                     /locus_tag="ACG8XG_001066"
     CDS             13898..14341
                     /locus_tag="ACG8XG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0772995.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(14845..16263)
                     /gene="nhaC"
                     /locus_tag="ACG8XG_001067"
     CDS             complement(14845..16263)
                     /gene="nhaC"
                     /locus_tag="ACG8XG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0772996.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(16666..17889)
                     /locus_tag="ACG8XG_001068"
     CDS             complement(16666..17889)
                     /locus_tag="ACG8XG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0772997.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(17920..18759)
                     /locus_tag="ACG8XG_001069"
     CDS             complement(17920..18759)
                     /locus_tag="ACG8XG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0772998.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(18776..19456)
                     /locus_tag="ACG8XG_001070"
     CDS             complement(18776..19456)
                     /locus_tag="ACG8XG_001070"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0772999.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(19458..20531)
                     /locus_tag="ACG8XG_001071"
     CDS             complement(19458..20531)
                     /locus_tag="ACG8XG_001071"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773000.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(20587..20825)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(20868..21101)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21178..21849)
                     /locus_tag="ACG8XG_001072"
     CDS             complement(21178..21849)
                     /locus_tag="ACG8XG_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0773001.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(21936..24584)
                     /gene="mgtA"
                     /locus_tag="ACG8XG_001073"
     CDS             complement(21936..24584)
                     /gene="mgtA"
                     /locus_tag="ACG8XG_001073"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0773002.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(24926..25100)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            25273..25710
                     /gene="ndk"
                     /locus_tag="ACG8XG_001074"
     CDS             25273..25710
                     /gene="ndk"
                     /locus_tag="ACG8XG_001074"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0773003.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(25732..26598)
                     /locus_tag="ACG8XG_001075"
     CDS             complement(25732..26598)
                     /locus_tag="ACG8XG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0773004.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(26758..28851)
                     /locus_tag="ACG8XG_001076"
     CDS             complement(26758..28851)
                     /locus_tag="ACG8XG_001076"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0773005.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         8430 a         6191 c         5136 g         9483 t
ORIGIN      
        1 aaaagaggct gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg
       61 tttattctga aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt
      121 ttgaataaac gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa
      181 gcatcccagt ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta
      241 aaatcttatc taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt
      301 aaaaataaat gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga
      361 ctagcatagc ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt
      421 ctttccgctg ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa
      481 tagctgctgt accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa
      541 gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt
      601 tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt
      661 caatcggtaa gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt
      721 tgatcacgac tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct
      781 gcgccttgat cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca
      841 agacgtaaat aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt
      901 gtttttttgc tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca
      961 tcaccatgat gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca
     1021 atataatgtc gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta
     1081 ctacaataaa taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac
     1141 tagcattttc gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca
     1201 tcacgatcga tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc
     1261 tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac
     1321 cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt
     1381 ttaaatctgg acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg
     1441 ttcctacgag tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca
     1501 taataccaaa tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta
     1561 aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc
     1621 ttttatcaaa aagtataaca aacctttttt atttttactt aattaataaa aactttaata
     1681 gaaatataat tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca
     1741 aattacacca ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg
     1801 atccatgaac attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct
     1861 agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa
     1921 agaaatccat gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc
     1981 tacttattta gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac
     2041 gatgcgtgaa tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag
     2101 cggaaataaa aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt
     2161 tggctttcct ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat
     2221 ttatcagatt ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt
     2281 cagtttcttc tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt
     2341 gcttttgctt ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg
     2401 gttaccaaga cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct
     2461 tctgctacaa gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc
     2521 gtaccgccta agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc
     2581 gttgtgatcc caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga
     2641 gaatagatat gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca
     2701 aaaactggat cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg
     2761 atagtcacac tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg
     2821 gctgtttcag tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat
     2881 gtagcaacga agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta
     2941 ccgtcagaga tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca
     3001 tttccgatca ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat
     3061 gggcgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg
     3121 actggtaaag ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat
     3181 aatgccaatg ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc
     3241 gtacgaccag tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg
     3301 aacataacca tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg
     3361 ccaagtaccc agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg
     3421 atgaagaaaa tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata
     3481 tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca
     3541 gcgaagatga cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag
     3601 tttttcagtg gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca
     3661 ccaagagttg gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg
     3721 tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct
     3781 gcatcaagtg ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc
     3841 ccagtaccga caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc
     3901 gatttgattt tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt
     3961 tcgtttacac ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa
     4021 agtttttctc ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact
     4081 ttcccaggtc cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga
     4141 atcgctttta attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga
     4201 gtcatgcagt ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt
     4261 tttgccagtt cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt
     4321 cccttttaca aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt
     4381 tgtcaaaatc atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc
     4441 agctgtttcg taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag
     4501 tttttcattt gttggtttga catcgaccat caagttattg aatactttac ctataccaat
     4561 catactgata gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg
     4621 agtagagcct gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt
     4681 actgatttcg gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc
     4741 gtaatctaat ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat
     4801 atcatttttt gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc
     4861 ggccccttcg acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat
     4921 ttccggttct acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc
     4981 acttgttccg gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt
     5041 agcaatcact ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc
     5101 ttctttgttc attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc
     5161 ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct
     5221 tcttttctta taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct
     5281 tctttgataa ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt
     5341 aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc
     5401 atacgcgtag ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg
     5461 cttcgtatga catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg
     5521 taaaattctt catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa
     5581 gcaaattgct cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg
     5641 ttacttgcta atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa
     5701 gttggcaatc cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg
     5761 acaaggagac cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc
     5821 cctgtttcgg gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct
     5881 gcttctttca attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc
     5941 aattgatgtg accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct
     6001 gttacaccaa ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat
     6061 gcctctcctg aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt
     6121 tcgatcaatc gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa
     6181 ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa
     6241 gaatcgttca taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt
     6301 catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata
     6361 attaaatttc atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa
     6421 aaggcactgc taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat
     6481 aattactcat aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta
     6541 ggcatcggat gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga
     6601 tagacatttt cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca
     6661 tctaagactg actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag
     6721 ttctcatctt tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca
     6781 tcaacggtca gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt
     6841 tgaagagggg aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct
     6901 agtttctgtg cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact
     6961 aatccttgtg cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct
     7021 ccttcgttgg caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag
     7081 attctctcaa gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc
     7141 actagtaaca tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag
     7201 acatccaaat aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat
     7261 ttagccaatt ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa
     7321 acatctcttt ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg
     7381 tttttctctg caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa
     7441 ctaatgctca gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc
     7501 agaagataat ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc
     7561 tgatgaggct cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga
     7621 agatcttcta cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa
     7681 tcgatatatt cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga
     7741 ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta
     7801 ttcactcgtc tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct
     7861 cgggaacgag accattcggc tttttcagct gctgttttct ttagcctact gacttctttt
     7921 ttcaattttt cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag
     7981 atagagaaat tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc
     8041 actacttcat cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt
     8101 ttcaaataag ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg
     8161 attaacggga aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct
     8221 ccccccgata gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg
     8281 agctctcttt cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt
     8341 aaacgttctt tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact
     8401 tgcccttgat aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg
     8461 tttcgtccga taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat
     8521 aataatgtgc cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt
     8581 ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa
     8641 gggttggata cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct
     8701 gcacactgct tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt
     8761 aatttttcaa tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag
     8821 aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta
     8881 agatttttgt tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac
     8941 ccaaatgaat acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc
     9001 tacttccgca aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt
     9061 atggaacttt tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat
     9121 ctgcttgttc gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa
     9181 ccatcttcgc tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg
     9241 gaatatcatc taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt
     9301 gtttatctaa gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag
     9361 cagctccttg ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt
     9421 tagaaacaag aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc
     9481 cttctgtgta cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc
     9541 tacacccgct tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt
     9601 taataagttc tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat
     9661 caagtagatc taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc
     9721 aagttgtcaa taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg
     9781 tacgccatgt tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg
     9841 caatcggcac attgattttt cttcctttac gatcttccca gccatttaga actgcctgat
     9901 ttagttcaaa tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct
     9961 gtatccctca ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat
    10021 gctattttct tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa
    10081 aacatgaaaa tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta
    10141 catcaaagcg gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt
    10201 cgtacaaatg ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc
    10261 tattcaccag aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca
    10321 aaagccaatc aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact
    10381 tatgttttgt gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca
    10441 aagaaaaata tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa
    10501 gtcacattgt taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca
    10561 tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat
    10621 aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc
    10681 acgattttgg attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt
    10741 cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa
    10801 gaagaagtgt accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac
    10861 ccatctatgt accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat
    10921 ggtgccattt ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg
    10981 acccaattag tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga
    11041 gaatcagctg aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa
    11101 aacacaactc tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc
    11161 aaaatgtagg ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa
    11221 atagtggaat cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat
    11281 agctctgata ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca
    11341 cgttctcgaa gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga
    11401 ttatccgtca ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct
    11461 tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac
    11521 tcttcataag taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg
    11581 gtcgtaacat ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca
    11641 ttattcgttg caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt
    11701 ttcgaataaa atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa
    11761 tgataaatct catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct
    11821 gctttgaagt gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt
    11881 cgagccatct ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac
    11941 tgttgtctag ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct
    12001 aaagagcgga tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca
    12061 tttttgttca tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta
    12121 aatagtaaaa agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga
    12181 aaatatgatg aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt
    12241 gtaattgtct gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat
    12301 ataaattttg attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa
    12361 aaacaactta gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt
    12421 ctatttaaag gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt
    12481 ttgattggtg ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc
    12541 ggtggcgaca cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct
    12601 gctgatttta ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct
    12661 gttgcagctg cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt
    12721 tttatgttgg ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa
    12781 acagaatatt ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct
    12841 acttatcttg tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg
    12901 actgaccatg gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta
    12961 gttatctatt cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat
    13021 cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac
    13081 atcattcctt tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat
    13141 gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc
    13201 aattttgtga ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca
    13261 agaattttgt tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta
    13321 aacaagcatc aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt
    13381 gtagctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg
    13441 tatacagtat tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga
    13501 aaaggaacat tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt
    13561 ttccttggta tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac
    13621 ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga
    13681 aatcgataga aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt
    13741 tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt
    13801 aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg
    13861 atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt
    13921 gttgatgatc ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc
    13981 gctcgatcat atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt
    14041 catggcagtc atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt
    14101 tatgatctta gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca
    14161 aactactgtc aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat
    14221 cctggcaagt aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat
    14281 cattcaggca caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg
    14341 accattatcc gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata
    14401 agaagaagct acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca
    14461 agtaataaga acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt
    14521 accgcagatg caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac
    14581 ctgagtatta gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta
    14641 ataccgaggg tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt
    14701 ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt
    14761 aataccgaga aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc
    14821 ttgtcacaat ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc
    14881 ttgcggtttc acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa
    14941 cacaggagaa agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa
    15001 tgtacttgct acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc
    15061 accatcttct agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc
    15121 tttaaatgca ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac
    15181 tccgatactt gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga
    15241 tactcgttcc atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa
    15301 agttagaata atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag
    15361 tgaatcgatt tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa
    15421 cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt
    15481 caacaaaatc gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa
    15541 aggaatcagt gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg
    15601 aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga
    15661 aacaacaaat gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc
    15721 atcaacaata gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa
    15781 aacggcacct gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc
    15841 aataccgaaa aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct
    15901 acctacgatt gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt
    15961 gaacccaatg accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga
    16021 accgactaaa ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc
    16081 ttcgtgcagc caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat
    16141 cgctgctaag atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc
    16201 taaaacaata accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc
    16261 catgataatc tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt
    16321 actccgccgc aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac
    16381 taaggacgaa tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca
    16441 aacttttatt caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca
    16501 cagcgaccac cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg
    16561 tacgaagttt accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt
    16621 tctgaaaatt atttttataa ctttcacaca caagattatt ttttactaga aagtagcgcc
    16681 ctcttgtttt tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt
    16741 tggctctttc aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga
    16801 agcaaggcct ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata
    16861 tgtctcagag attgctaccg taggctggat tgttggtatg acttggctga catttcctac
    16921 atcagaagaa ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc
    16981 ttcttttgca tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac
    17041 tgcattttgg aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac
    17101 gatattttcc actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata
    17161 gaaacgtcca gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat
    17221 cccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac
    17281 ttggatcaat gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc
    17341 gacaccgtaa aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata
    17401 acgaaaagct ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac
    17461 aaagctgcct ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg
    17521 gatttctcca ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta
    17581 agtaccaaac aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc
    17641 caaaaaggca attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg
    17701 gtgtccagct acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc
    17761 tgctgaggaa taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag
    17821 tgcttgatat tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt
    17881 tgttgtcata aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc
    17941 ccatgcaggc acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt
    18001 ttgataagcg tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac
    18061 gatgatattc acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga
    18121 agcaggatta aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc
    18181 tgacaaagca cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt
    18241 ctcggtaata tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc
    18301 agcagtttgc aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac
    18361 ttttgctccg tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg
    18421 tgcattgaca gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc
    18481 taaaaagaat tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt
    18541 tggttgattg taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg
    18601 gtctttcacg ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt
    18661 ttcttttttg tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc
    18721 tacaaatgta atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat
    18781 gtgtaatttt tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa
    18841 tcaataatgt agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag
    18901 caagattccc taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt
    18961 tgatgatagt caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac
    19021 gtaaaatgat accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat
    19081 caacttctag cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca
    19141 atggcactaa agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg
    19201 ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa
    19261 gtatttcaaa tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca
    19321 cacctaagat gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg
    19381 tttgcttcgt cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt
    19441 taaagaaatc agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg
    19501 tttttttgta aaatatttat ttttacacca gatttgatca aataatccaa tgcttttttc
    19561 cgctgtttgg tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat
    19621 aactctacat tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg
    19681 attagaggtt cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg
    19741 atctgatcgg caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta
    19801 gctgttcgaa taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgctg
    19861 ctaccttttt caacgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg
    19921 acaacttgca tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt
    19981 tttaacaatg caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca
    20041 cataataata ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt
    20101 ccccccgaca gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt
    20161 agttcattca ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa
    20221 tcaacgttgt caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg
    20281 atggcctttc tttcttttcg caattctttg ggagagagct gactcagatt cgtctgattg
    20341 acaatgactt ctcctgctgt tggtttctgc agcaaattca tcacacgaac taacgtactt
    20401 ttccctgcac ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct
    20461 acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc
    20521 aattcaatca ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt
    20581 cattttcttg aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca
    20641 caactcctct tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct
    20701 tacatctatg ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc
    20761 ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca
    20821 aataatattc aaaaaaaaag tccttttact aacataaact atgtcagtaa aaggacgacg
    20881 tatcgcggta ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga
    20941 gagattggcg ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt
    21001 attctccaag gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc
    21061 tgcaaagttt ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta
    21121 atctacatga ggaaaactga aaagtcaagt acgaaattca aattattttt tctatattta
    21181 tttttctgtt tttttattta acatttcaat aacagttcga agatcttttc cttccgcaag
    21241 aagtttcaat ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat
    21301 caaaacaacg cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac
    21361 tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg
    21421 tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa
    21481 tttaaagtga tccaagatat ctgttgctct tttcgtaaca gtggtcgtag caatgcctag
    21541 tttcatatga cgttctttta agtctgccaa tatttctttt gcatatggtt ttaagtggat
    21601 ggctccgctt gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga cttcctgtac
    21661 tttttcaggg ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc agttttttcc
    21721 gccccaagcg tcaacgattt cttttgggat atggatatca aattgctgaa atccccaaag
    21781 ccagccatca cgatacgttt tttctgtgtc aactaacaaa ccatctaaat caaatattgc
    21841 acctttcatc tatacattaa tcctttccaa aacaatacac atagtttagc acgactggtc
    21901 caaaaagaac agaagcaaag aaccagcctt ctcgtttata agagttcttg gaatcgacgg
    21961 atatagattt tcttcacagc tgtcacaagc gccagataag cgatgactgt agcaaataac
    22021 cataaccagt aattgccagg aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa
    22081 ggaaggacag aaccgacaat aattcctaat gtagtgataa caaacatggc aaatgttgcg
    22141 ttgctttgga taaatggtac tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc
    22201 catagtgatt cgacaaacca tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa
    22261 ctatccaatg tatggaaact tccgcccact acagcaggac aaataacaaa atacatcaac
    22321 aagtaagtag taatatcaaa tatcgaactt gttggaccaa agtaaaccat gaattcccca
    22381 atactagatg cattccattt ttttggctcg tccaaatatt ctttatccat ccgatcccat
    22441 ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa acaaaagttg taaaggcagc
    22501 attggtaaaa atggcaggaa cgtacttgca atcagcacgg aaaacatatt gccaaaatta
    22561 gaacttgctg tagccttgat atatttcatg atattcccaa aagtttctcg tccagaaaga
    22621 aggcctcttt ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc agattctttc
    22681 gcaatatcca cagctgtatc tacagaaata ccgatatcag ccactttcat tgcaggagcg
    22741 tcattgatac catctcctaa aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc
    22801 aaacgtgctt tttgctgagg attcaatttt acgaatactt catggtcttc agctacttgt
    22861 gctaattcat gatcactcat ttccgagatt ttctcaccgg taaccaattc attgatctct
    22921 agacctactt gacggcacac tgaacgtgta actaattcgt tgtccccagt cagaactttt
    22981 acagctacac catgttcttt cagagctttc aacgcttgtt tcgttgtttc ttttggtgga
    23041 tcaaggaaag caagataacc aatcaaaacc atgtctgact catctttgac tgaaaattct
    23101 ccaacgacgc ttggattggt cttttgtgcg acaccaatca ctcgtaagcc atcttcatta
    23161 agtgactgta cttttgcaag tacggcttct tttcgttctt ttgttaatgg tgtaacagta
    23221 ccgccaagat caataaattt agaaatgttc agcatttctt caatcgcacc ttttgtgatc
    23281 atttgtgttt taccagctgg atcagcaacc acaacactca tacgacgacg ttcaaaatca
    23341 aaaggaattt catccacttt ttgataattg atcttatctg tatctaaagt ttctgttgct
    23401 tcatcaatga tagccacatc taacaaattt ttcagccctg tttgatagta gctatttaga
    23461 tacgcatggc gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga
    23521 atgattttat cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc gattgcacca
    23581 aagttctgga tggcattcaa atttttgatc actgttcctt ttttggccat cacacttgct
    23641 cctttgacca aattcgtcgt aacgatcatt ggaagcattt caggtgttag accaactgct
    23701 acagatagtc caaaaagaaa agcttctagc caatcccctt ttgtaagccc attgatgacg
    23761 ataactgctg gagccatcaa tgacataaat ttgatcagta gcatggatgt tttatggatg
    23821 cctaattcaa agcttgattg aattggtttt tcagatagcg tttctgctac atgaccaaat
    23881 agtgtttcat ttccaacaga aacaatgatt ccttccgcac ttccactgat gacattactt
    23941 cccataaaga caagattttc gtaacttgtt tcgctttgct gatcgtcttc acatactttc
    24001 gcatgctttt ctactggata gctttcccct gtcaatgctg cttgggaaat gaataaatcc
    24061 tttgcggaca ataaacgcat atccgctggt atcatatccc ctgctgaaag ccgaacaaga
    24121 tctccacaga cgatttcttc cataggaatc tctgtgtact ttccattacg ttttacagct
    24181 gccgttactt tcacaagtga tttcagtttt tcagccgctt gattggaacg tacggactga
    24241 atcaacgtca ttgtcccgct gacgataacc atagtgaaga taatggctac accgaaaaga
    24301 tctttatctc caggagctgc taatacatat tccgtgatga atgaaataat tcctaatgcg
    24361 atcaacacta atgtaaatgg cgtgatgtat gctttcaata tttctacaat gaatggtgtt
    24421 tttttgccat atgaaatcgc attttcaccg taattttctc ttgaacgttc gatttgttct
    24481 tctgtcaatc catttgctga tgcatggaat agttcaaata attcttgttt cgtttttcca
    24541 gcaaaagctg catagatttt ttgtgtatct ttcttttttt ccatctcaat ctctcctttg
    24601 tgtccaaaag acagctgttc tcgctgcttt tggtctttag ctattcatcg gatcgataga
    24661 gaaaaaactg aaaggggctg agaacagata gataatagcc ctggcctttc ctctagatcc
    24721 ttgtttttcg aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt
    24781 catgacttcc aatcattttt cgaatagtta tttttccaat tagaccacct cctttttatt
    24841 tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt
    24901 ttgaaaaagc atattaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca
    24961 acgtaaaaag cagatacctg cttttagcta ccttatgaaa agccttatct cgacaattcc
    25021 tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc
    25081 ctcacctaac aaatcttatt cggttttcct tcgttaatat aacaactaaa aaagaggatg
    25141 tcaaatcaat tttccatgtt tttcatactt tttttaaaat acacacgtta tattcctgtc
    25201 tcacgcggtt ttatttgtct cattttccac ctcccgctat aatagaaata tagtaggaaa
    25261 cgaggtgctg ttatggaaag aacacttgtt attatcaagc cagacggcgt gagacgccat
    25321 ttagtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaag
    25381 tttgaaacta tgactcctga attagcaaaa gaacattatc agcacttgac tgagcgcagt
    25441 ttttttgatg aactgatcga ttacatgact tccggtcctg tcgtttatct tgtgctggtc
    25501 ggcgaggaag tgatcgatat cgtccgtaag atggttggag caacaaaagc tgccgatgct
    25561 gttccaggta cgatccgagg agactacgcg ctgccgggga ctgaaaacat cattcacgct
    25621 tctgattccc gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc attcgatata
    25681 aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg tctagttgct
    25741 gggttcagca atggccagca gttttttctc taaggacctc cgttgattct ttcctaacat
    25801 atattcgagc actgtgaaga attcttggta gtctttcgtc ttttcttccc ctaaagcagc
    25861 agaaatcaat ctttgataat aatctgccat caatgtttca tagatcactg tacttctttc
    25921 tttatgtgct aagctccgtt tcatatcttc attcacacta cgtgcctttt tcaaccgacc
    25981 attttctata taaaacgaag tcaggttcat taaaaaagta aagagatact gctcatgatt
    26041 gttcaactgt gctaatcgtc gcattctttt tttgattttc aaataattgt tctcaatgaa
    26101 ccgatcactg aatatccaag gaaaatcagc tagaatagat agttcaaatt gaccatagtc
    26161 tggtatctgc atcaagtatt cttcaatgat tcggcattca taacgtaaaa attcaaattc
    26221 tgtctcagat acttctgttt tttcgaccat gtgaagattc aaaaccaaat aatacagatc
    26281 ttctgttttc ttaaaatgcc tacggttatt gctgatctct ttataaggat caaagctgtc
    26341 ttcttgtacc cgatctagca ttcgtctttt tttctttaac tttattggtt catccagctg
    26401 gtttcgcatc cattcatatt ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa
    26461 tgttacaaat gacatttttt tcactgaatt tgtctcaatc ccgactagag aagtcttaaa
    26521 acagattcct tccgctaatt gggactgtgt caacttcttg ttgactcgaa gtttcttgat
    26581 caattcacca tcatacataa tagttctcct ttttaattta aatttttcca cattcaataa
    26641 atagatacgt taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt tttcataaaa
    26701 aataccaacc ttcaagcatt agaatgttgt ctaactactg aaggttggtt tgattgttta
    26761 ttgattattt ttttgctgtt caacgtctgt tcttgttact ggatataagt tggttgttgt
    26821 tttattacca ttttttgaaa agacacttgt cgaatgttcg cctttttgct tttcgattgc
    26881 ttcattacgt tccgcttcat ttttatagtc atattttttc ggatcaatcg tctcaaatcc
    26941 gtttggcaca taaaaccgca agagattttc ctgattcagc ttatctgaaa gacttaacgc
    27001 agtcttgact ttttcctgat ctttggagat ttcctcatcg attcctgctg ctttcgtatc
    27061 gatgatttct cctgtctgat tattgtaagc ttttcctcct aggacggtgt atttgggtgt
    27121 cacaaagttc ccatttcgaa aagcaactac ttgatcatgc tgagaagata ataagtctgt
    27181 accaaaatga agataatctt tatcatcgat tcccaataga tgtagtaatg tcggcaaaac
    27241 atcgatttct ccaccgtatt catgattgac tgtcccttta gtataaccag gcatatggat
    27301 catcaatggc actcgttgca tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt
    27361 accaagagcg cttgctaaat ctttattatc tgtattcgag atcccaaaat gatccccgta
    27421 aatcacaaag attgaatttt ggtaaatacc agattttttc aaatacgtaa aaaattgttc
    27481 caatgattga tccagataat gtgccgttcg aacataatta tctactgtgc tattgccagt
    27541 attcaaagat ggaaagtcga aattcttgtc atccgtataa taaggcgtgt ggttggttac
    27601 ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca agatattggg cagattctct
    27661 gaataaatct ttatctaata tcccataacc taacgtttcg tcggattcat caaaataaga
    27721 tcgatcaaag aaattgtcat aacctaagtt tttatataca tgatctctat tccaaaaact
    27781 agctacattc ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg
    27841 tgcggcttga aagacattgt ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc
    27901 aaatgtgcca gtttccaaca tattttcagc atcacttgtc tttccctgtc ctacttcatg
    27961 aaagaaattg tcaaagctga tcgttgcttg attctggaaa atactattta aaaacggtgt
    28021 gacttcttgt ccgtctacct tcatattaat caaaaactgc tggaaacttt ctaaatggag
    28081 aacgatcaca tttttccctt tagcgatccc aaatgtttcg ggatttggct ctgcataatg
    28141 tttctttgta taatctaaaa cttcatcaat cccattactg cttgcatgcg cgcgaacact
    28201 gctagtcatt cctgttttga ttccgtcata aatagtatac gcatcgattc ctagatattt
    28261 gacaatatat gaacggtcaa atgttcgctg cagcaattgt ggacggttgg cttcgctcag
    28321 cgctagattt cctgaaaaaa taagccctga aagacaggta acagcgatag ccatcggttt
    28381 gccgacaggg ttgcttttga taggtatttt cttgaccttc aaccaaacca gtagcccaat
    28441 gaacacagcg atatccagcc agtagatgac atcatggggc ttcatcaaag aaaaggagct
    28501 tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca gatttgactg taatgaaatc
    28561 tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa agcgtattta aaatatcaat
    28621 gatcaaaaga atagctaacg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa
    28681 caataaggta gtcgcaattg ggttgatcca caagataaag tactggatcg taccttccac
    28741 acctaatgaa aaatctgcat agtaggcaat gatcgtcttc agccagaaaa agaaaacagc
    28801 tgtcaaaacg attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac
    28861 ttctctctat tcacattttt tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag
    28921 ttgttatctt aagatttttt tattctttct aaagattatt ccgtccactt ttcacagcat
    28981 aaaaccgctg cgatgtaagc gattaaacta atgaagagat aaaagtcttg tggtgaccgc
    29041 ttcactccac ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa gaggctgtga
    29101 caaaattctt gtcgcagctc cttatttctt gatgcaggac agctttttca caacttcttc
    29161 cttaaacggt gttctcctgc caactcctcg gccacaagtc acaatattta taaaacatga
    29221 gaagctgttt tttgaaattg
//
LOCUS       DAZKSR010000028        29142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000028 DAZKSR010000000
VERSION     DAZKSR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.028"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1076)
                     /locus_tag="ACG8XG_001077"
     CDS             complement(27..1076)
                     /locus_tag="ACG8XG_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0773006.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1158..1847)
                     /locus_tag="ACG8XG_001078"
     CDS             complement(1158..1847)
                     /locus_tag="ACG8XG_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0773007.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1953..2321)
                     /gene="rplL"
                     /locus_tag="ACG8XG_001079"
     CDS             complement(1953..2321)
                     /gene="rplL"
                     /locus_tag="ACG8XG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0773008.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2374..2877)
                     /gene="rplJ"
                     /locus_tag="ACG8XG_001080"
     CDS             complement(2374..2877)
                     /gene="rplJ"
                     /locus_tag="ACG8XG_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0773009.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3081..3208)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3287..3976)
                     /gene="rplA"
                     /locus_tag="ACG8XG_001081"
     CDS             complement(3287..3976)
                     /gene="rplA"
                     /locus_tag="ACG8XG_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0773010.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4080..4502)
                     /gene="rplK"
                     /locus_tag="ACG8XG_001082"
     CDS             complement(4080..4502)
                     /gene="rplK"
                     /locus_tag="ACG8XG_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0773011.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4845..5513
                     /gene="sdaAB"
                     /locus_tag="ACG8XG_001083"
     CDS             4845..5513
                     /gene="sdaAB"
                     /locus_tag="ACG8XG_001083"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0773012.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5526..6398
                     /gene="sdaAA"
                     /locus_tag="ACG8XG_001084"
     CDS             5526..6398
                     /gene="sdaAA"
                     /locus_tag="ACG8XG_001084"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0773013.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6489..7589)
                     /locus_tag="ACG8XG_001085"
                     /pseudo
     CDS             complement(6489..7589)
                     /locus_tag="ACG8XG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7681..8568)
                     /locus_tag="ACG8XG_001086"
     CDS             complement(7681..8568)
                     /locus_tag="ACG8XG_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0773014.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8645..9190)
                     /gene="nusG"
                     /locus_tag="ACG8XG_001087"
     CDS             complement(8645..9190)
                     /gene="nusG"
                     /locus_tag="ACG8XG_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0773015.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9291..9461)
                     /gene="secE"
                     /locus_tag="ACG8XG_001088"
     CDS             complement(9291..9461)
                     /gene="secE"
                     /locus_tag="ACG8XG_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0773016.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9485..9637)
                     /gene="rpmG"
                     /locus_tag="ACG8XG_001089"
     CDS             complement(9485..9637)
                     /gene="rpmG"
                     /locus_tag="ACG8XG_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0773017.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9799..10443
                     /gene="cbpA"
                     /locus_tag="ACG8XG_001090"
     CDS             9799..10443
                     /gene="cbpA"
                     /locus_tag="ACG8XG_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0773018.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10499..11002)
                     /locus_tag="ACG8XG_001091"
     CDS             complement(10499..11002)
                     /locus_tag="ACG8XG_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0773019.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11015..11061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11599..12564
                     /locus_tag="ACG8XG_001092"
     CDS             11599..12564
                     /locus_tag="ACG8XG_001092"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0773020.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12663..13835)
                     /locus_tag="ACG8XG_001093"
     CDS             complement(12663..13835)
                     /locus_tag="ACG8XG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773021.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13822..14967)
                     /locus_tag="ACG8XG_001094"
     CDS             complement(13822..14967)
                     /locus_tag="ACG8XG_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773022.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14981..15913)
                     /locus_tag="ACG8XG_001095"
     CDS             complement(14981..15913)
                     /locus_tag="ACG8XG_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773023.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16030..17109
                     /locus_tag="ACG8XG_001096"
     CDS             16030..17109
                     /locus_tag="ACG8XG_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0773024.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17106..17724
                     /locus_tag="ACG8XG_001097"
                     /pseudo
     CDS             17106..17724
                     /locus_tag="ACG8XG_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17834..18061)
                     /locus_tag="ACG8XG_001098"
     CDS             complement(17834..18061)
                     /locus_tag="ACG8XG_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0773025.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18164..20752)
                     /locus_tag="ACG8XG_001099"
     CDS             complement(18164..20752)
                     /locus_tag="ACG8XG_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0773026.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20772..21431)
                     /locus_tag="ACG8XG_001100"
     CDS             complement(20772..21431)
                     /locus_tag="ACG8XG_001100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0773027.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21480..21983)
                     /gene="trmL"
                     /locus_tag="ACG8XG_001101"
     CDS             complement(21480..21983)
                     /gene="trmL"
                     /locus_tag="ACG8XG_001101"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0773028.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22204..23070)
                     /locus_tag="ACG8XG_001102"
     CDS             complement(22204..23070)
                     /locus_tag="ACG8XG_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0773029.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23179..23820
                     /locus_tag="ACG8XG_001103"
     CDS             23179..23820
                     /locus_tag="ACG8XG_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0773030.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23882..25243)
                     /gene="mgtE"
                     /locus_tag="ACG8XG_001104"
     CDS             complement(23882..25243)
                     /gene="mgtE"
                     /locus_tag="ACG8XG_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0773031.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25261..26160)
                     /locus_tag="ACG8XG_001105"
     CDS             complement(25261..26160)
                     /locus_tag="ACG8XG_001105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0773032.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26162..26959)
                     /locus_tag="ACG8XG_001106"
     CDS             complement(26162..26959)
                     /locus_tag="ACG8XG_001106"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0773033.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26937..27641)
                     /locus_tag="ACG8XG_001107"
     CDS             complement(26937..27641)
                     /locus_tag="ACG8XG_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0773034.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27772..28347
                     /locus_tag="ACG8XG_001108"
     CDS             27772..28347
                     /locus_tag="ACG8XG_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFQ0773035.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28465..29142
                     /locus_tag="ACG8XG_001109"
     CDS             28465..29142
                     /locus_tag="ACG8XG_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0773036.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8577 a         6176 c         5238 g         9151 t
ORIGIN      
        1 tttagacatt ttcctctagg aaaaaattaa ctagcaccac gggtaaatac agtattattt
       61 cggttgcttg ctgcttcacc tagcccttgt actcgtgttt gattggcgac ttgaccaaat
      121 tgattcccga cgtctaataa aataatagct aaaagcaaaa tggccaaatg gcttccgcca
      181 accaataaag caataaagct tattgtttgc ataacaagtc ccatatacac gatttttctt
      241 tcagaataag aatctgccag tcgtcccacg ataggtgcag caactgctcc agacaaacca
      301 aaaatcgcta atatccctgc ttcgaatgtt ccccaattgt aggcgggact gctgatataa
      361 aaaatcaacg tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc
      421 gcttctctta atagacgttg agaagcaact aaatgcggca aacttttaag tgaatcaagg
      481 tatgaaacag ctgtactact ttgctggaca gaacgcggca ttttccagta aagcagaagt
      541 gtaagaattc cgactgcaca taaagcaaat aaatagatca tccgccaaga aaaaagactg
      601 gcaacaatac cagataccgt ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat
      661 gtacccatga ccttccctct tgcttttggt cccgctagta ccgcaccgta aggaatgatg
      721 atttgcggca cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac
      781 gaaggcgcaa aaaaagcggc caacagagag acggcagata acgctaccat tcgaatgatc
      841 aattttggac gatccaccac gtcccctaaa ggaactaaaa acagtaaccc taacgcatag
      901 cctaattggg tcaacatcgt caatatcccg actgcacttg tgctaacaga taaactagct
      961 gctacttttg ttccaatcgg ctggatgtaa tacatattgg caacaacaac gccacaagtg
     1021 attgataata gtaaaataag cgaagttgtt aaaggtgttt tttcattact cttcattgat
     1081 ttcccccatt tcctatctta gtttacaact tatggaaaag attggaaata gaaaaataca
     1141 actattcgcc acacttatta tttgtgcttt tgttttcctt gttttttctt gcgatcttta
     1201 taaatgaaga caactaaccc tactagcaca gtaacacttg caaccaacgt gactgcagtt
     1261 ttgttatatc cgtgaaacag agcttctcca ccacacgcat acaacacaga cgaagggatg
     1321 actccaatca cgatcgacaa tatgatttgc tgtagaggca atttcatcgc atctgcagca
     1381 aaattgacca tagaagatgg aataaccggt atcatatagc ctaacattac acctattttt
     1441 ggatgtttca tttgtcggat tgcttgcacc caatgattcg tttcggtttt cttatctaaa
     1501 aattttaagc gatgcataag gaaaattgca gtgatgttac caagagcatt acccaaaatg
     1561 ttgatgatcg cacctgtcag tggtccaaag ctcaaaccag caatgactcc aactactgaa
     1621 gtaggaatac ctggaacagc acacatcacc gctgtcaata cgatcaagag tatcgatgca
     1681 aaagtcccat gcgaacgaat atgtgcgagt agctctgcct tactcgctct cggatttaaa
     1741 aataattgga tgtctgtctg atattctaaa tacaaccgat agcccactac cagtatcaga
     1801 atgattccca acgagatcaa catcaatcgg attattttct ttttcatatt tcctcctgaa
     1861 aaaaatctat attcattaaa taatagttta acataaaaac aaaaaagcac cgaaatccaa
     1921 cataggatct cagtgctttt aaatagtaaa aattatttaa cagttacaga tgcgccaact
     1981 tcttcaagtt tagctttcaa ttcttcagct tcttctttag atacgccttc tttaactggt
     2041 gcaggagcgc catcaactac agctttagct tctttcaagc ctaaaccagt tgcttcacga
     2101 actgctttga taactttaac tttttggtct ccagctgcag ttaattctac agtgaattct
     2161 gtttgttctt cagcagcagc agcgccacca gcagcagcta cagctacagg agcagcagca
     2221 gatacgccaa attcttcttc aatagcttta actaggtcgt taagttctag gatagttgct
     2281 tcttttaact cagcaacgat gttttcaatg ttcaatgcca ttataatttc ctccgtttaa
     2341 ttgttctttt ttatttaatg ctttatgcgg caattaagct gcatctccat cttctttgtc
     2401 tgccactgct ttgacagcgt aagccacgtt gcggactggc gcttgtagta cagatagcaa
     2461 catagataga agtccttcgc gatttggcaa ttttgccaat gctgtgattt cttccaatga
     2521 agagacttta ccttcgatga taccgccttt gatttctaat gcttttgctt ctttagcaaa
     2581 ttcgtccatg attttcgctg gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg
     2641 tccagtgaat acttcgtcca tgccttcaag gccagctttt ttagctgcac gtgaaagaat
     2701 agaatttttg ataactttca tttctacgtt agcatcacga agttgtttac gtaaacgtgt
     2761 tacttcatca actgttaatc cgcggtagtc aacgactact actgatgcag ctgctgtaaa
     2821 tttttcgctt acttcatcaa cgattgctgc tttttttgcg ataattgctt cactcatttt
     2881 tgagtttcac ctcctgaatt ttgatggaag agttttttgc actgaaaagg caagtcactt
     2941 ttatagttac tttgctcaac cagtattatt catcacctat tcacttcgtt catagtgatt
     3001 ctactcgctt tgctcgaaag gaaactcctt tcgctttgct caaccagtat tgttcatcat
     3061 ctcttcactt cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag
     3121 ggactattga atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta
     3181 ctgtcttcgg tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag
     3241 tcaagaatta tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt
     3301 ggtcaacatg gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac
     3361 ctttagctgc tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa
     3421 ctaatttttc atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt
     3481 ccacacggta agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg
     3541 taactgtacc agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac
     3601 gacctacagt agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt
     3661 tttggatttt ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt
     3721 cttttgcttt gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg
     3781 gtaatacaac agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt
     3841 atgcaacttc aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg
     3901 cttcggcaac aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt
     3961 tgctcttttt agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac
     4021 gtgttctata tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat
     4081 tattctacag tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca
     4141 acgttagctg cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta
     4201 gaaactttcg caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct
     4261 tttttcaata gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg
     4321 tatacagaaa taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat
     4381 tcttttgtga atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga
     4441 gctggagttg ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc
     4501 acgagacata cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc
     4561 acgttttatc tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta
     4621 cagtatagca aatttatttg ttttttcaag atttttttct gctattttta cattttgctt
     4681 gaataaacgc taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta
     4741 tgttacactt gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag
     4801 acccttgtac aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt
     4861 atagaagtgt ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg
     4921 ccggagctgc tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg
     4981 tagatattta tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg
     5041 cattagttgg cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga
     5101 aattggcata tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac
     5161 atccaaactc tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca
     5221 tttcgatcgg cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa
     5281 gcatgggtac tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag
     5341 tgactagtat actttctgag accaatacga atatcggcac tatgacagtg acacgggaat
     5401 caaaaggtga aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg
     5461 taaaaagact caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta
     5521 aaaaaatgtt tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag
     5581 cagaattgat gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg
     5641 agatcatgga aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg
     5701 ttacctccgt cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg
     5761 aggcagactt tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta
     5821 atgaagtcaa tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg
     5881 ttgttcctgg tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat
     5941 tggaattctt atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca
     6001 gtggtgcaga agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag
     6061 ctttggtttg tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat
     6121 tgaaaaatat gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg
     6181 tcaaacgtaa tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg
     6241 gtatcgaaag tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc
     6301 aaatgccgtc gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta
     6361 aacgacttgc ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta
     6421 ttaagagatt agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc
     6481 gttttttatt aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca
     6541 agttgcatcg tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct
     6601 gccccaccta gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca
     6661 cttgccagag ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt
     6721 acacgtttgt tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca
     6781 tctaattgac gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg
     6841 attactgcat cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct
     6901 ttgattgttt tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg
     6961 attccttttc cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc
     7021 attggtaatg gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt
     7081 gaatctgccg ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac
     7141 tcattgaatc tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct
     7201 gcatcggctg cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca
     7261 gcagcagcta ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc
     7321 gctgatggcg cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa
     7381 tcggctgagt cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc
     7441 gtattttctt tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct
     7501 gcttccatgc gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga
     7561 tgttcttcat tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca
     7621 tataaatcat actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg
     7681 ctattctttc caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat
     7741 actatgagag gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt
     7801 caacaacgca tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt
     7861 ttcatctagt tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat
     7921 ttgctggtaa tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt
     7981 cagagactgc gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt
     8041 ctgaacttgt ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac
     8101 tcctcccatg gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata
     8161 caaaagacaa gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa
     8221 caatacttgg accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg
     8281 tccatctgtt cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg
     8341 ccgcaacatc cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg
     8401 aatagtggtt ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc
     8461 agtagtattt atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa
     8521 agctgctgca gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct
     8581 ttccatatag aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc
     8641 tttattaatc gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa
     8701 acatatcgat gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt
     8761 ctaatccagc aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat
     8821 cattttgtcg caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta
     8881 atggtgctgg tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt
     8941 tgcggacaac ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga
     9001 atgttttatg aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag
     9061 gaacaacgac acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat
     9121 ttgcttttac tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa
     9181 aagattccat tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa
     9241 gtttttcttt cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa
     9301 atccagccaa ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata
     9361 atagaagttt cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt
     9421 acttgtttga tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta
     9481 ctgtttattt cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga
     9541 tttccaaacg ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac
     9601 aaacggaaca agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga
     9661 atcagactta tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa
     9721 actatctatt gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag
     9781 acaattggag gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca
     9841 accgttacag agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac
     9901 cgttgtgtgc cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg
     9961 catatctatc gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg
    10021 aaaaacgcta caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc
    10081 aaagagcttc cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca
    10141 catagtacgt tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta
    10201 ctgacaattg cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca
    10261 aaacacagca gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga
    10321 cgtacattga tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac
    10381 cacttagaga aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca
    10441 taatcaaaaa gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt
    10501 agacacgctt ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag
    10561 ccatgacgat aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac
    10621 ctgttggaac taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca
    10681 atactaaacg catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag
    10741 aaagcaatgt ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat
    10801 tatagacgat gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat
    10861 gattcaaact ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa
    10921 tagttaacgc tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt
    10981 gtctgctttt cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga
    11041 tggttgatga accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt
    11101 tcaatccgtc ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa
    11161 aaatcgtggg atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag
    11221 acgaagtagg atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt
    11281 gggaatttga gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg
    11341 acatgccgaa ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt
    11401 ttttgtgttt ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca
    11461 agaattaatt ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga
    11521 aaaagcttgc taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt
    11581 gaatagcagg aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc
    11641 agcggaaatg gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt
    11701 gactatacac ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg
    11761 gcaaaagaaa cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc
    11821 cttttgatca tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag
    11881 atggaagtag aattcccagt tgcttatatt ccagcaggtt ctggaaatga ttttgctcga
    11941 ggggcaggcc ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca
    12001 ccagaaaaag tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct
    12061 gttaataatt tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc
    12121 acaaaaaagc gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc
    12181 cgtgctttat tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta
    12241 agtttttcaa acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc
    12301 gcgattgttc caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga
    12361 atcaatttct ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa
    12421 tcgaaatatt tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa
    12481 catggtcaag aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca
    12541 actaaacagt tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac
    12601 cgtttattcg gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc
    12661 agttaattct ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat
    12721 tggatcattg aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat
    12781 tctgcaaatg tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac
    12841 caatatgttg gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt
    12901 acaaacaccc caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg
    12961 gtttctgagt tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa
    13021 aacaagaaag tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact
    13081 aacagaacca ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta
    13141 aaggttactt tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg
    13201 atctctttgc gattaaacgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac
    13261 aacccataag acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta
    13321 tatcccgcac ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata
    13381 gctgcatcga ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc
    13441 acttgctgtg taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc
    13501 gccatctttc ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt
    13561 acacgattaa aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg
    13621 actgtttcct tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca
    13681 ctaatcaagg cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca
    13741 aaaaagaatg tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct
    13801 ttaagcaatg tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc
    13861 atttcctaac gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact
    13921 agccaaatca gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt
    13981 taattgacca acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat
    14041 cataccagca aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac
    14101 cagattcatt tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga
    14161 ccccagagcg gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat
    14221 aaaacgaaag acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc
    14281 caaattagcg attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt
    14341 tgacgattga tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag
    14401 aatcgccatt ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc
    14461 atgaacttct tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac
    14521 gttctccact tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat
    14581 gacgtttttc atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga
    14641 accatcaatg agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac
    14701 ttccgatagt tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac
    14761 tgtggtaaaa ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat
    14821 cgtttcaaga ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa
    14881 aagaattgga aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt
    14941 gcgatattta taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc
    15001 cagtaatctc taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac
    15061 tgatcttttc ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag
    15121 ttgttacttg gagctgacct tttttataag cgattccatc agaagctatt gttcgctgaa
    15181 tcgctgacaa cacattttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc
    15241 gtaccatatc ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga
    15301 tcacgatctg gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg
    15361 ttgctccttg tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt
    15421 ctacagctac tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg
    15481 cgatgttcaa ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt
    15541 tttctaatcc cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg
    15601 aaacatataa accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg
    15661 caacgtcttg aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg
    15721 ttgtcccgaa caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga
    15781 tggcagtcga tttcccactg ccgtttggac ctaacagacc taagacttct ccttcttgta
    15841 cttgcagatc aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa
    15901 tttcgataat catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag
    15961 tgcaagaaaa gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag
    16021 gagtctgcta tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca
    16081 ttgcttttgc tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc
    16141 gttttattca ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt
    16201 attctatttc ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg
    16261 cgagtgatcg cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg
    16321 atttcgacgg ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt
    16381 ttttcctttt acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt
    16441 gtctccaaag atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg
    16501 gccctgtcac aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc
    16561 cgagacatcc atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg
    16621 cttgaaatga tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc
    16681 gttcacactg ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc
    16741 tcttttcaac actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa
    16801 ggaaacttgt ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa
    16861 gaagccttaa ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga
    16921 acgttgccgg ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat
    16981 tctgctcccg gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa
    17041 gtccatattt tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga
    17101 caccaatgat aacagtagtc attattgatg atgatccatt tgtgacgaaa tcgttacaaa
    17161 ccattttaga aagcacgaaa gagatccgtg tgttaggcat tggtcattgt gccaaggatg
    17221 cgcgaaaccc atcgtcccga tgttttgctg acggacatcc gcatgcctaa acaaacgggc
    17281 atcgatgctg ccaaagaaat tttggctaaa tttcccaaag ccatcctttt gcttttgacg
    17341 acattcaaag atgaagaata catccgcgaa gcattttcca tcggtgtcaa agggtattta
    17401 atcaaacaga acttgcaagc gattattcct tctgtcaaat ccgcttataa tggacaggcc
    17461 gtatttggta atgaaatcat cgagactttc actcaaatga tgaagaataa accgacgatt
    17521 gatagtcatg cttatgcttc ttttagtgaa cgtgagcttg caatcatcaa agaagtagct
    17581 gccgggaaaa acaataaaga aattgcagat gccttgtact tgagtgacgg cactgttcgc
    17641 aattacatca gccaattact agaaaagcta gatttacggg atcgcacgca attggctatc
    17701 tatttttatc agcatttgca ataagtctta tctaacacaa aaaaacctga atgatgctcc
    17761 ttcgcatcat tcaggttttt ttcacatctt ctattcaatt gtcccgcaaa ccatttcctc
    17821 acgattgaaa gaatcaagga ttcgcagggc tgccaggatt cgttggattg ttcggcgtgt
    17881 taggcggaaa aataggcgcc ttgtctaacg ttttttctag ttttgattct tcacgatcgt
    17941 ctcgttctac atcaggactt tcattcaatt tgaattcttt ttccatctca tctaactttt
    18001 taaagtcatt gccagattct tttgctaatt tcgctaattt atcttcggtt tctttagcca
    18061 taaagtaacg cccccctttt gatttcagct tagcatatca cactgaaaaa acaaaagaaa
    18121 acgctaacca acgatcattt ttagctattt ctcatcttta aaatcaagca ttttttgccg
    18181 gtataaaatc gtagggagaa ctgttttcca taccaatcat cggatctact aactggttat
    18241 cgatcacttc tgctgtaaga acgaaatctt tagaaacagc aggttctacc tcttctttct
    18301 tttcttctcc aacaaatgaa gaaaacagat ctggttccac tgtttccatt acttggtttt
    18361 tcgtttcatt cgtcccactg tcatcttcta caggtagctt ttcttttaca acccgtggct
    18421 cttttgtata gttcccatct tcaaatggat catttccgac caaagaatct tcataagctg
    18481 ctagtttggt ccggagagtg atattcattt tatcattatc catgatcctt gatttcaacg
    18541 tagtcatccg gcttgctgat tcattcgcta cacaagtgac ataggcttgc tcttccccta
    18601 acaaaatcaa cagttcctta ctgcgagtca ctgccgtgta gagcaaatta cgctgaagca
    18661 tccgctgata ttgatggacc atgggcaaaa taaccatctt gaattcactg ccttgtgatt
    18721 tatggattga gcaacaataa gaaagagtga ttttattcca ttcgtttctt ttataagtga
    18781 cctcatttgc atcaaactgg ataactaatt catccacttt atcttcagaa tcttttgcca
    18841 agatgatacc cgtgatttct cccatatctc cattgaatac attcaattcg ggagtattga
    18901 ctaattggag gactttgtct cctatccgat aaacggtgtc gttccaatgt acttcctttt
    18961 ttccaggttt ccctggattg aatatctcct gcatcatttt atttaacgca tcgatccctg
    19021 caggtccccg atacattggt gctaatactt ggatatcttg tgcacggaat cccttatttt
    19081 tcgctttttc cacaatttta gagatcaagg gttcaatttg ataagcatca cttcggaaat
    19141 aggaacgatc tttttgattc tttgtgaaat cttttggcag acgtccttct ttgatctcat
    19201 gggcaagagg aataatacta gaaccatcac cttgccgata aatctgattg agttccattt
    19261 tgggaatttg ttcgatttcc aaaagatcat ggagtacttg accaggaccg acagagggaa
    19321 gctgatcttt gtctcctact aagataactt gcatattcgt cggaatcgct ttgaataatg
    19381 tgttagccag ccatgtatcg accatagaca tctcatcaac aatcaaaagt ccgccttcta
    19441 attctttcgt actgactgaa ggtgtttttt cccgtccatt cagccccaga agacgatgga
    19501 tcgtactggc aggcagacct gtcgtttcgt tcattcgttt agctgctcgg ccagttggtg
    19561 ctgcaagtag aataggaaaa atttcttggg tatattttga aggatccaat tccagtccgt
    19621 tcaactctgc aaaaagattg acgattccgt tgatcacagt agttttcccc gtacctggtc
    19681 ctcctgttaa aataaataga ggggaacgga ttgcttcagc gatagcttgt tcttgcgaag
    19741 gtccatactg tatgccgaat attttttcga tacgccgaat attcttctgc acttcttttt
    19801 caggataatg gatttctttt ttacgactga gaaggcgctg gatcgaatta ccgatccccc
    19861 attcagaaaa gtacaaactg ttttcgtaaa gattagtctc ttcttgttgt atctttcctt
    19921 cttcaacaag ttggataatt acagtagcaa cctgatcagg ctggatttca attgggcgac
    19981 ttgtttctaa aatacggatc gtttgctgaa gcaatgtctc cgccggtacg tatgtatctc
    20041 ctgtttgaac tgcatgctga aaaatctgat gcaagatagc tgctcgaatt cttctcggtg
    20101 aatctgctgc aataccgatt tgttcggcta gattatctgc acgtttgaac ccgattcctt
    20161 ctatgtcttc gactagctgg tacggatttt cttcgatgat atccaaagac tcgttcttat
    20221 aggcctggta gatactgaaa gctaactgac taccgaaccc gtaacgattc agtcccatga
    20281 tcacttgatc catcccgtgg tttagccgaa tcgtctcagc aagcatttct ctttttggct
    20341 tcgtcaaacc ggagatttgt tttaataccg aaggatcagc aatgattttt tcgatagcat
    20401 cttctcccag caattctaca attttttctg ctgttttctt cccgattcct gggaattttt
    20461 cgctggaaag atacgctacg atcccatttg cagttgttgg cttttcttgc tcatatcttt
    20521 cgagtttaag ctgccttccg tatttaggat gatccactaa ttctccataa aaacgataag
    20581 tctcattttc ttgaatatcg ccgaaacttc cggtgactac aatctctttt tcacggtaat
    20641 cagctgtggt ttcactgatg tgtaccaaaa gcactttgta aaaattacta ggattttgaa
    20701 aaaaaatcgc ggcaactgta ccaacgaaat aaggcatttc ttctggctgc atcggaagcc
    20761 gcctccttct tctatagtaa aggttctggt gctgtttcag ttcctaaaaa atctgtttgg
    20821 ttccatactt tcagttcata tggcaataat ctgcaaggct cttcagtctc taaaatcgtc
    20881 aaactactat tgaacaatcc acccatttca cgcaactcac ttagctcttt tcctgtcatc
    20941 cattgaatgg ctgcagttaa agaagctcca tgtcctacga ataaaagtgg gccatcttta
    21001 tgtcgggcaa ccgcatctgc tacaacagtt tcgatacgtg tgattgccga ttcaatcgtt
    21061 tctccgtgaa aaggagaagg atcatacaga tctaaatgat tacgcaaatt cggcagatta
    21121 tccggatatt tctggtacat ttcatcgatc gactgacctt ctaaagtacc cagccctagt
    21181 tctcttagct tatctgtata aaaaatctct accggacgag caagctcttt gttgatacct
    21241 tctgctgtat cacgtgctcg ctttgctgta ctggaataaa ttgctttaaa aggaacatat
    21301 tttacctttt tcccaaatgc tttgatttct tcgtaacttt gtggcaaaag cggagaatcg
    21361 ccattgctcc cttgaaaacg tctttcctga ttccattgtg tcttcccatg tcgggtaaaa
    21421 tatagttcca tacgcagcaa actcctgtct ttcttttatt ttactctata ataaactgtc
    21481 taatccagct tgtcattttc atagtgatgc gtcaattcta aattagggaa tccttgctgg
    21541 cgaagtgctt catacactat cattgcagct gtattcgata aattcaatga gcggacatgc
    21601 tcatcattca tagggatgcg taaacacttt tcttcgtttt cacgcataaa tgtttctggc
    21661 aaaccggttg tttcttttcc aaataaaaag aagtgctctt ctccatcagc aaaattttcg
    21721 tcactatata ctttatgggc aaattttgag atcaaatgta gtttacgatt acctaaaaat
    21781 gttaaaaaag cttctaaatt actgtggtaa gtaatattta catcattcca ataatccaac
    21841 cctgcccgtt ttagatgttt gtcatcagtt gaaaaaccta gtggctcgat taaatgaagc
    21901 ggagtattcg tggctgcaca cgtacgggca atatttccag tgtttgcagg gattaatggt
    21961 tcaaataaaa cgatatgatt catcaggatc attcctctca tatttatgtt cgtttctatt
    22021 ttctgacaat ttgatttttg cataaaaaaa aacgtatcaa ctcggtgtca gtcactgcga
    22081 gtcgttacgt tagtgaagct cttctcacta acttttatca gaacaggaac ctgataaaaa
    22141 ccaatgaaaa aacaacttgt tgctaatata acaccctcaa cttttttttg caacctctac
    22201 tcattaaaaa ctgctagaaa aacgttttct cttagttcct tttaataatt ctatgtcaat
    22261 tgtaatcttc ttcaaaggat tcttatataa gtctgctttg attcgctctt ctacctgcca
    22321 ttctaaaggt gacatggcca aaagatcttt ttgattttcc tctggtatct caaattcata
    22381 ggttacacgc tgacgttcag ttaatttcat tgcttcagca aacttggcta ctacacgttc
    22441 attggaataa ctctgttttt caggttgatt cggataaaaa gcctgacgta gttcctttaa
    22501 ataattttct tgtggcacca ccttcaacac ttctccatct tctgagagta cacgttggaa
    22561 ttcttggtaa tgagaaggcg aaaagatatt taaaaccgcg ttgaatgttt gatcagcaaa
    22621 cggcaaattg gtcaaatcag ctacacacca aaatgcttcg gcagattgtt ctgtagctgc
    22681 gtagactcct tcttttgaaa tatcaaatcc aacagcattt tgaatctttt tcaattttga
    22741 aagttcattc aagaagctac cttctccgca gccaacatct aatagcgaga cttcttcagg
    22801 taaatgctgc gcaatagtct caataacggg ctgatacatg ccagattgga tcattctacg
    22861 ccttggaacg aacattcctt gatcgtattc tgttttaacc gcatgggata aaaaataaag
    22921 tgttcctttt ttggaaaggt caaaacggtg attatttttg catatcaagc cattatcaga
    22981 cttttttact tcttcttggc aaaccggaca gcggaacagc tgatgatttt ctgccaaaaa
    23041 ctggcgtcct cggtcaattt tttttaacat agttccccct catttctgtt ttatcttacc
    23101 gttagatgct cactttggca actgctaaaa atatgataca atgcctagaa gaaagtgaaa
    23161 gataaaggag aagattagat gatcaaagaa ttttgcgctg aaaactacac atctattcct
    23221 ctagcaattg ctagaggagc cgatcggatc gaattgtgtg acaacttagc ggtcggtggc
    23281 acaaccccga gtactggtgt gatcgaagaa acgttaagtt atgctgggga aaaagatatc
    23341 cccgtaatga cgattattcg tcctagaggg ggcgacttcg tctataacga tatcgaattg
    23401 aaaatcatgc atactgattt gattgaagca aagaaattag ggacggatgg cgtagtattt
    23461 ggttgtctta ctccttcagg ctggctagac gaagaggcct tagaactatt gatcgaaaca
    23521 gcagaagggt tgcagatcac cttccatatg gcttttgaca gtattcctga agatcggcaa
    23581 ttcgaagcaa tcgattggct ggtagaacat ggtgttgacc gtatcttgac tcatggcggt
    23641 cctgctgaca aaactattgt agaaaatttc ccacggctta aagaattgat tgcctataca
    23701 gatggccgct taatcatttt gcctggtggt ggtgtcactg cagagaatgc agcacatgta
    23761 gctaaagaat tgaacgtaag cgaagtacac ggaaccaaaa tcgtggatct tcagttataa
    23821 tcttataaac aaaaaaacat ttgcaacagg cataatgctt attgcaaatg tttttgtttt
    23881 tttacataaa atagcccata aataaagaag caatattgaa ataaatcagt acactcgtca
    23941 aatcgctcaa agtcgtgata aagggaccgc ttgctactgc tggatcaaat cctagacgat
    24001 ccattagcat cgggattaaa ctacctgcaa gattcgccac tgtgattgca aatagcatag
    24061 ccatccctat gacaaacccc aagataaaat tatgtttcca aaaacctacg attagaaaaa
    24121 tcgtgatccc cgtaacagct cctgtgacaa cgccggtcaa gacttcagcc aaaacaagtc
    24181 gaacaaatcc atgatctttc tcatcagaaa tcgcaagtcg acgaacagcc acagctagcg
    24241 actgggttcc agcattaccg gcagttcctg taatcagcga aatgaagact gctaaaatac
    24301 tcgcttcact taccaagtct tcatagtgac tgattaaaga agcggtcgac atacctaaaa
    24361 acaacagagt gatcagccaa ggcaaacgtc gagacgctgc ctttactgga ttttcattaa
    24421 tttcttccac attgaccccg gcaagtcctg aataatcgct agcagcttca tcatcaataa
    24481 cgtcgatgat atcatcgact gtgacgattc ctaacaaatg atcatcataa tcggtaacag
    24541 gaagagctaa gaagtcataa tctctaaatg tttgtgccac atcttcttgg tcgtcgccta
    24601 catgaactga aagcactcgt tcgctcatca gatcagaaat cattgcgtcg tcatcattca
    24661 cgattaggtc gcgcagagaa ataaccccta ctagctgacc ttcttgatta acgacataga
    24721 tgtaatagat tgtttcagct acatctgctt cgttcttcag cacgtacatc gcagagcgaa
    24781 cagtttgatt ggctacgatc gaaacaaatt cagttgtcat gatcgcgcca gccgtttcat
    24841 cctcgtaatg gagaagttcc ttgatttcac tggcatcgtc tgccgacatc aaacttaaat
    24901 atttagcaat ttgcttttta tccagcgtat taagcaagtc aacagcatta tctgtataca
    24961 tctcagaaag catatcagct gcatagctag gacgcatttc atctaggtat tctttcatat
    25021 gttcgttgtc ttcttcaatc acatcgaaca tatccgctaa ttctttaggc gacagatagg
    25081 aatagatcag ctgcctgttc tcttctgtaa gagattggta gaattgcccc tgctcataga
    25141 tatgcatctc taaaaaactg tttctgaatg cctgaatatt attttccctt aaatcttgag
    25201 tcaactgggc aaatctttcc tctaattctt gattttcatc caaaccaatc tcacctcgcc
    25261 ttattcttca aaccgtctca caactgcctc catgtctgcg gctaaaggct gcttgaactc
    25321 cagcaactct tctgtaaatg gatggtaaaa acgcagatca taacaatgaa gggcttgtct
    25381 ttttattccc agatccattc gcccgccata catatcatca ccgagcaggg cacagccaat
    25441 tgcggaaaaa tgaactctta tttgatgtgt ccttcctgta tgcagctgga tatcaactaa
    25501 cgcgatttct gttagtcgct tctctatcca gtactcagtc tgtgccgaac gtccttcatc
    25561 agagaccatt cgcttcaata gcgaagaata atctcgggcg atcggcaaat cgatatgtcc
    25621 atgtgccgat aaatctgaaa gccttccact gactaaggcc tgatattttt tcacaaactt
    25681 cttttcacga agttgcatat ctaattttgc gtgcgcaaat ccatgctttg caaaaagcat
    25741 caagccagat gtatcccgat ctagtcgcgt caccacatgg attacttggt tttcgtaacc
    25801 ttgtttttta taataagcct ttacacgatt agccattgtc ccatttggat ggtattgggc
    25861 aggaatagac gaaacgcctg ctggtttatt tactaccaga acgtgttcat cttcatacac
    25921 gatatcgatc ggtgtttcat ccaaaagtac ggtttcatgt tctccttcag caggaatcac
    25981 aatggttacc ttatcatcct tagctagaga aaacaagaca ttttctactt gatcattaac
    26041 tttgatttga ccgccttgaa attttatttt cgctaacaga ccctttgaga tccctttttc
    26101 ttttaaaaaa tattttactt gctgagaatg atctttctca taatcccatg taaactgcat
    26161 atcaatcctc actaataaag gcatctttca cacgatgcca aaaatgcata tgtcgataag
    26221 aagcaaaatg gatccgttct tctgctattc gataataaat tgagcggata tttgcttttg
    26281 cgacattcaa ttgatcaacc gttacaagat aatcttctgt attttccaat tttatttcta
    26341 gccattcatc atgggcaatc acgatcggag aacccaaagt acgaaacact cgattattca
    26401 gtgaagcaat ctctgccaac tgaaaggcat tgatactagg atggatcaca gcaccgccga
    26461 cacttttatt atatgctgtc gaaccagtag gcgtagaaat cgtcaatcca tctcctctaa
    26521 aactttcaaa cagctcgttt ttgatgtata catcggctac catcgtacga ttggcccgtt
    26581 taatcgttga ttcattcagt gctaaaaaat gctggtccgg tgtttcatcc aaataactga
    26641 tccgaacatc cagcaaggga taactaacac tttgttcccg gttcgttcgt aagctgtcta
    26701 ctagttcttc cagctcgtaa tcccgccaat ccgtgtaaaa ccctaaatga cctgtatgca
    26761 cgcccaagaa acgcacatca ttcagacaat gactgtagtg atggaacgcc gacaataatg
    26821 tgccgtctcc gccgactgag atgaccaatt ctggctcatg atcatctcgt tgaatccctg
    26881 cttgttctaa aagtgcgttc aaacgttctg tcacttctat agtctttgct tctttattat
    26941 gaacgatcgc tactttcatc ccctgcctcc tctctttcgc tttctttctt caaaagcgtt
    27001 cgatagtaga catcatctga ttttcctctt ccatgggaga ataaccgctg tgcttcttgg
    27061 atttcttcac ggatactgga catctcttca tccaactgat aggcagcttc tgccgcacgc
    27121 tgaagtcgtt cactcatttc ttctggaaaa acgccttgat atttataatt cagcgaatgc
    27181 tcaatcgttg cccagaaatt catcgcaaga gtccgaatct ggatttccgc caaaattttc
    27241 ttctcaccgc tgatcagctg tacaggatac tcgatcacca gatgataaga acggtagcca
    27301 cttgcttttt tatttgtgat gtaatctcgt tcttggagaa ctttcatgtc ttttctttta
    27361 cggatgatct caacaacttg atggatatct tctacaaact gacacatgat ccttaacccg
    27421 gcaatatcag acatttcttc ttctaaacgt cccattggaa tatgccgtaa ttgtgatttt
    27481 gttacaatac tatcgactgg cttgactctc ccagtgacaa attcaatcgg gacatgtcga
    27541 ttttgttcac ggaactgttt gcggatcccc cgcagtttca ctttcaattc tgatacagtt
    27601 tgttcatatg gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac ctctacttca
    27661 ttaacattca ttgtttattc taccatatat gcaaagtttt ttcgtactct tgatttatgc
    27721 attgaccaaa aaaagaaaat taggcaaaat agacaaaaag gagtcgatca aatgacacaa
    27781 atgcttgaaa tcgaatataa atctatgctg acaaagcaag agtacgagat attcatcgct
    27841 cactatcaat taacagataa agatttccgt gtacagacta atatttattt cgataccgtt
    27901 gatgaacagc tgaagaaaca aaattgtggc ctacggatcc gtttcgttgg tcatcaagct
    27961 gaatatacat taaaaacacc tgctgaaaaa gggcgtctcg aaacaacgga tacctttata
    28021 acagaagaag cagaagcatt tatcttcgaa aaacgtctgc ctcgttcagg cgcagttttt
    28081 caaaaacttt ctgatttgaa tatcgatcct gcttcattaa tccaaaccgg aaagcttaca
    28141 acaaaacgag ctgaattccc tattgaagaa ggacttttag ctattgatga aagctgggga
    28201 gacaatctac acgattttga actggaacta gaggtagggg atgcatctgt gggaaaaatt
    28261 gcttttataa attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc
    28321 caacgtatgc ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca
    28381 tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata
    28441 gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg
    28501 gaatactgtt tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca
    28561 ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg
    28621 gatgtcatgc aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc
    28681 tcaatgactt attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa
    28741 ggccgccgtt ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct
    28801 caagaacttg ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa
    28861 gatcgtcagt ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa
    28921 atgggcatca caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt
    28981 ggtgttttag tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa
    29041 acaacagata actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag
    29101 ttccattcaa gaactagcca tttacacttg ctttctaatt aa
//
LOCUS       DAZKSR010000029        28706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000029 DAZKSR010000000
VERSION     DAZKSR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.029"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8XG_001110"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8XG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0773037.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8XG_001111"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8XG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0773038.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8XG_001112"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8XG_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0773039.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8XG_001113"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8XG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773040.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8XG_001114"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8XG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0773041.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8XG_001115"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8XG_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0773042.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8XG_001116"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8XG_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773043.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8XG_001117"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8XG_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0773044.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG8XG_001118"
     CDS             complement(7139..7417)
                     /locus_tag="ACG8XG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0773045.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG8XG_001119"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8XG_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0773046.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8XG_001120"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8XG_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0773047.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8XG_001121"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8XG_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0773048.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8XG_001122"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8XG_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0773049.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8XG_001123"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8XG_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0773050.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8XG_001124"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8XG_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0773051.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8XG_001125"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8XG_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0773052.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8XG_001126"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8XG_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773053.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8XG_001127"
     CDS             13967..14695
                     /locus_tag="ACG8XG_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0773054.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8XG_001128"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8XG_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0773055.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8XG_001129"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8XG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773056.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8XG_001130"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8XG_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773057.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8XG_001131"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8XG_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0773058.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8XG_001132"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8XG_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773059.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG8XG_001133"
     CDS             16394..16606
                     /locus_tag="ACG8XG_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773060.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG8XG_001134"
     CDS             complement(16639..16833)
                     /locus_tag="ACG8XG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773061.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG8XG_001135"
     CDS             complement(16846..17028)
                     /locus_tag="ACG8XG_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773062.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG8XG_001136"
     CDS             complement(17025..17165)
                     /locus_tag="ACG8XG_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773063.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG8XG_001137"
     CDS             complement(17655..18020)
                     /locus_tag="ACG8XG_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773064.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG8XG_001138"
     CDS             complement(18060..18362)
                     /locus_tag="ACG8XG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0773065.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG8XG_001139"
     CDS             complement(18362..18661)
                     /locus_tag="ACG8XG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773066.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG8XG_001140"
     CDS             complement(18654..18818)
                     /locus_tag="ACG8XG_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773067.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG8XG_001141"
     CDS             complement(18824..19093)
                     /locus_tag="ACG8XG_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773068.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG8XG_001142"
     CDS             complement(19105..19854)
                     /locus_tag="ACG8XG_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773069.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG8XG_001143"
     CDS             complement(19851..20543)
                     /locus_tag="ACG8XG_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0773070.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG8XG_001144"
     CDS             complement(20549..21220)
                     /locus_tag="ACG8XG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0773071.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG8XG_001145"
     CDS             complement(21213..21554)
                     /locus_tag="ACG8XG_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773072.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACG8XG_001146"
     CDS             complement(21732..22430)
                     /locus_tag="ACG8XG_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0773073.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="ACG8XG_001147"
     CDS             22517..22987
                     /locus_tag="ACG8XG_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773074.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="ACG8XG_001148"
     CDS             complement(23030..23209)
                     /locus_tag="ACG8XG_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773075.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="ACG8XG_001149"
     CDS             complement(23196..23534)
                     /locus_tag="ACG8XG_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0773076.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="ACG8XG_001150"
     CDS             complement(23550..23753)
                     /locus_tag="ACG8XG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773077.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="ACG8XG_001151"
     CDS             complement(23768..24544)
                     /locus_tag="ACG8XG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0773078.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24843..25163
                     /locus_tag="ACG8XG_001152"
     CDS             24843..25163
                     /locus_tag="ACG8XG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773079.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25181..25612
                     /locus_tag="ACG8XG_001153"
     CDS             25181..25612
                     /locus_tag="ACG8XG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0773080.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25742..26203
                     /locus_tag="ACG8XG_001154"
     CDS             25742..26203
                     /locus_tag="ACG8XG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0773081.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            26237..26872
                     /locus_tag="ACG8XG_001155"
     CDS             26237..26872
                     /locus_tag="ACG8XG_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0773082.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            26985..27620
                     /locus_tag="ACG8XG_001156"
     CDS             26985..27620
                     /locus_tag="ACG8XG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773083.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(27613..27747)
                     /locus_tag="ACG8XG_001157"
     CDS             complement(27613..27747)
                     /locus_tag="ACG8XG_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773084.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            27919..>28706
                     /locus_tag="ACG8XG_001158"
     CDS             27919..>28706
                     /locus_tag="ACG8XG_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0773085.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKF"
BASE COUNT         8429 a         5744 c         4492 g        10041 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca gtatctgagt aatactctat actaagagca taattaaata agacataaaa
    24661 acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg
    24721 tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc
    24781 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
    24841 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
    24901 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
    24961 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
    25021 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
    25081 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
    25141 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
    25201 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
    25261 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
    25321 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
    25381 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
    25441 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
    25501 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
    25561 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
    25621 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
    25681 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
    25741 aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag taactgatga
    25801 ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta ttaaaggaga
    25861 aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag gtatgagtaa
    25921 tggttatata caatttacct tgctaggcgg aaacgaaagt cgcggaggta tattagcggc
    25981 cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt
    26041 aaaaaaatat atagagaagc aacaatctat attagataat aattccaaag aaacacaaat
    26101 ttcaagtgca gatgaaatta aaaagtataa agagttgtta gatgaaggga ttattaatca
    26161 agaagaattc gattttaaga aaaaagaatt gcttggttta taatattcaa tatcaaaaga
    26221 gaaagaggat ctattaatgg acggtatatt tggttttact ggtattgttt tatttattat
    26281 aggtttaatc atgttaattg taagatttat caaaaaaaca aataagaaaa cacctatgat
    26341 tctccttatt atcgggatta tttttactac cgtaggtttc tcgctttcct catcaaacga
    26401 ggcaaatgac aataacaaac aggaaagctc atcatctgat actcaaacta ctacatctaa
    26461 cagcagcaat aaaagcctaa ctcaatttga agaattttta gagacaaata atcatgactg
    26521 gggtgctttc ttagatagtt attattctat tactccagct actgagcaaa atacagcttt
    26581 tactaaatat attagtggta aaacatatac ttttgaagga actgttatcg aatcaatgac
    26641 tactagaata gccattatag cagacaaaga gtacgatgat aaaagttgga atgatatttc
    26701 tgcaacacct aaagtttcat atgtaatttt tgcgaaggac ttaaacaatg ctgatacctt
    26761 tacaaagggc gataaagtaa cttttacagg agaaattagt tcaagaggat cgaacataga
    26821 aaaatcctat gctcaatggg atatgattaa tagtaaagta agtaaaaaat aaatgaacca
    26881 cgccccacta agagttagat tgtgcacaat aataaaacct atacaaaaga gtgccatcag
    26941 gagtcacaat gcttgtaaag gaaatgacaa aataggaggt aactatggat aatagcaatg
    27001 aaaaactaac caaaaaagaa tatcttgaat taatagttga aggcggtatt ggagcaattc
    27061 cttatattgg tggcgttgtg cagtcacttt attttgggtc taaaaacgag aagcgtttca
    27121 aacgcatcga gaaattctac aaaaatttaa acgaatcatt agaaaacatt cgcgatcaaa
    27181 ttccagaaga tgttttcaac ttagagaaca aggaccaact cattggaata ttcgaaggga
    27241 taaatgacga ggtagaaaaa tcaaaggcac aaaataaaat agatatgtat aaaaatcttt
    27301 ataagaattg tttacttcga attaacaacg cttcatggga taacgaggag tattatcttc
    27361 ttgtactgaa tcaattaaca tcgattgaga ttcaattgtt agcatgctta atgagtcgtg
    27421 gaaacgaaaa tttcactgga aatatcagca tgtctggcta ttcacaagag ctaatagatg
    27481 gtagcttaaa tagattatct gattttggtc tgttagaaaa aacgattagc tccatagtat
    27541 taggtgatgt cggcaaacaa aatatgggct acaaaatatc tcatttagga attcaattta
    27601 ttaactcaac tcttacttaa gttttctttc taatatgaca tctagaccta tcagcaatgc
    27661 aacaataaaa aaccagacat accaataatc aacaaataat ctaaactctg gaagttcagt
    27721 tattctttta aaaatcactt taatcataat aaacacctct gattggatga tctaatacac
    27781 atccaattat accattaaaa cgacaaatag cacatccttg cgccggcaag caaaaaggat
    27841 gtgcaaaaaa taataaaacc caaacaatgg gcttctttat agttcctatt gtatcagaga
    27901 aagagagctg attcaattat ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa
    27961 atttcataca aaaaagatga cggaaaatac acgaagataa gaaaatcagg atttaagaca
    28021 aaaggagaag caaaagatgc cgccaacgaa ttagaatata acttgaacaa aggccttaaa
    28081 ggggatcgca aaaatctatt attatcagat tactttgagg attggatgca actttataaa
    28141 gaaggaacag tatctcctat cacttataga aaatacgaag atacgttaat gaacataaag
    28201 aaatatatgc cagcggtatt gatttctgat ttagatagag tgggatatca acgcttttta
    28261 aataagtatg ctaaagacca tgtaaaatct accgttatta agtttaataa ccatattaga
    28321 gcgtcgttga aagatgccgt agaagaagga ttaattccgt ttgatccaac tagaaaagca
    28381 gtaatcaaag gaaaagattc attgaagcca aaagaagata aatatttaga ttatgatcaa
    28441 tttaaatctt taatgaaact cgtagaagaa aacctttctg cgcagtactc ttctcctatg
    28501 ctcgtgttag ttgctggtgc cactggaatg cgatttgctg aacttctagg attaacatgg
    28561 gaagatatcg atttcgaaga tcaaatcatc actattaata aaacatggaa ttataaatta
    28621 aatgaatggg gaaaaacaaa aaacgaaact tcaaatagga aaatttccat tgataaacat
    28681 acgattgatc tcttaaaaaa gtttaa
//
LOCUS       DAZKSR010000030        28615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000030 DAZKSR010000000
VERSION     DAZKSR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.030"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..694)
                     /locus_tag="ACG8XG_001159"
     CDS             complement(113..694)
                     /locus_tag="ACG8XG_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0773086.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(877..1503)
                     /locus_tag="ACG8XG_001160"
     CDS             complement(877..1503)
                     /locus_tag="ACG8XG_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0773087.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1525..2856)
                     /locus_tag="ACG8XG_001161"
     CDS             complement(1525..2856)
                     /locus_tag="ACG8XG_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0773088.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3011..3412)
                     /locus_tag="ACG8XG_001162"
     CDS             complement(3011..3412)
                     /locus_tag="ACG8XG_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0773089.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3498..3704)
                     /locus_tag="ACG8XG_001163"
     CDS             complement(3498..3704)
                     /locus_tag="ACG8XG_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773090.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3799..5301)
                     /locus_tag="ACG8XG_001164"
     CDS             complement(3799..5301)
                     /locus_tag="ACG8XG_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0773091.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5319..5774)
                     /gene="argR"
                     /locus_tag="ACG8XG_001165"
     CDS             complement(5319..5774)
                     /gene="argR"
                     /locus_tag="ACG8XG_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0773092.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5872..6489)
                     /locus_tag="ACG8XG_001166"
     CDS             complement(5872..6489)
                     /locus_tag="ACG8XG_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0773093.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6710..8083
                     /locus_tag="ACG8XG_001167"
     CDS             6710..8083
                     /locus_tag="ACG8XG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0773094.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8158..9270
                     /locus_tag="ACG8XG_001168"
     CDS             8158..9270
                     /locus_tag="ACG8XG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0773095.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9342..10979)
                     /locus_tag="ACG8XG_001169"
     CDS             complement(9342..10979)
                     /locus_tag="ACG8XG_001169"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0773096.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11147..11755)
                     /locus_tag="ACG8XG_001170"
     CDS             complement(11147..11755)
                     /locus_tag="ACG8XG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0773097.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12139..13290)
                     /gene="queG"
                     /locus_tag="ACG8XG_001171"
     CDS             complement(12139..13290)
                     /gene="queG"
                     /locus_tag="ACG8XG_001171"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0773098.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13309..14061)
                     /locus_tag="ACG8XG_001172"
     CDS             complement(13309..14061)
                     /locus_tag="ACG8XG_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773099.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14202..15152)
                     /locus_tag="ACG8XG_001173"
     CDS             complement(14202..15152)
                     /locus_tag="ACG8XG_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0773100.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15153..15992)
                     /locus_tag="ACG8XG_001174"
     CDS             complement(15153..15992)
                     /locus_tag="ACG8XG_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0773101.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15989..16735)
                     /locus_tag="ACG8XG_001175"
     CDS             complement(15989..16735)
                     /locus_tag="ACG8XG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773102.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16973..18343
                     /locus_tag="ACG8XG_001176"
     CDS             16973..18343
                     /locus_tag="ACG8XG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0773103.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18398..19294)
                     /locus_tag="ACG8XG_001177"
     CDS             complement(18398..19294)
                     /locus_tag="ACG8XG_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0773104.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19408..19578)
                     /locus_tag="ACG8XG_001178"
     CDS             complement(19408..19578)
                     /locus_tag="ACG8XG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773105.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19575..20252)
                     /locus_tag="ACG8XG_001179"
     CDS             complement(19575..20252)
                     /locus_tag="ACG8XG_001179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0773106.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20343..21248)
                     /locus_tag="ACG8XG_001180"
     CDS             complement(20343..21248)
                     /locus_tag="ACG8XG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0773107.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21423..22847
                     /locus_tag="ACG8XG_001181"
     CDS             21423..22847
                     /locus_tag="ACG8XG_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0773108.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            22893..23924
                     /locus_tag="ACG8XG_001182"
     CDS             22893..23924
                     /locus_tag="ACG8XG_001182"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0773109.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23931..25118
                     /locus_tag="ACG8XG_001183"
     CDS             23931..25118
                     /locus_tag="ACG8XG_001183"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0773110.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25274..26656
                     /locus_tag="ACG8XG_001184"
     CDS             25274..26656
                     /locus_tag="ACG8XG_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0773111.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26693..27013
                     /locus_tag="ACG8XG_001185"
     CDS             26693..27013
                     /locus_tag="ACG8XG_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773112.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(27213..27881)
                     /locus_tag="ACG8XG_001186"
     CDS             complement(27213..27881)
                     /locus_tag="ACG8XG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0773113.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(28070..>28615)
                     /locus_tag="ACG8XG_001187"
     CDS             complement(28070..>28615)
                     /locus_tag="ACG8XG_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0773114.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         7974 a         5846 c         5249 g         9546 t
ORIGIN      
        1 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
       61 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
      121 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
      181 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
      241 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
      301 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
      361 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
      421 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
      481 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
      541 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
      601 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
      661 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
      721 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
      781 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
      841 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
      901 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
      961 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     1021 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     1081 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     1141 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     1201 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     1261 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     1321 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     1381 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     1441 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     1501 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     1561 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     1621 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     1681 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     1741 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     1801 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     1861 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     1921 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     1981 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     2041 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     2101 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     2161 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     2221 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     2281 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     2341 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     2401 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     2461 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     2521 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     2581 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     2641 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     2701 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     2761 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     2821 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     2881 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     2941 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     3001 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     3061 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     3121 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     3181 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     3241 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     3301 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     3361 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     3421 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     3481 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     3541 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     3601 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     3661 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     3721 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     3781 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     3841 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     3901 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     3961 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     4021 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     4081 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     4141 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     4201 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     4261 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     4321 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     4381 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     4441 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     4501 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     4561 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     4621 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     4681 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     4741 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     4801 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     4861 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     4921 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     4981 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     5041 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     5101 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     5161 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     5221 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     5281 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     5341 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     5401 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     5461 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     5521 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     5581 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     5641 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     5701 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     5761 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     5821 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     5881 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     5941 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     6001 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     6061 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     6121 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     6181 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     6241 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     6301 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     6361 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
     6421 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
     6481 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
     6541 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
     6601 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
     6661 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
     6721 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
     6781 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
     6841 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
     6901 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
     6961 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
     7021 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
     7081 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
     7141 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
     7201 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
     7261 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
     7321 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
     7381 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
     7441 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
     7501 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
     7561 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
     7621 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
     7681 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
     7741 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
     7801 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
     7861 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
     7921 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
     7981 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
     8041 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
     8101 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
     8161 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
     8221 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
     8281 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
     8341 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
     8401 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
     8461 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
     8521 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
     8581 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
     8641 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
     8701 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
     8761 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
     8821 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
     8881 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
     8941 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
     9001 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
     9061 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
     9121 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
     9181 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
     9241 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
     9301 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
     9361 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
     9421 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
     9481 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
     9541 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
     9601 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
     9661 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
     9721 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
     9781 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
     9841 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
     9901 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
     9961 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    10021 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    10081 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    10141 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    10201 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    10261 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    10321 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    10381 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    10441 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    10501 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    10561 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    10621 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    10681 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    10741 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    10801 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    10861 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    10921 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    10981 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    11041 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    11101 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    11161 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    11221 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    11281 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    11341 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    11401 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    11461 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    11521 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    11581 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    11641 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    11701 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    11761 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    11821 tttcataggt aaaagaaaca tgacatttaa tgacaaaata acgaattatt taatatttgt
    11881 aacatttaaa caaatatttt tttgatacaa aaccataaaa gccttgttgt ataagcaaag
    11941 taatcgtttt tctatgtttt tttatttttg aatgaaaaac gcagattctg taacattcac
    12001 tgcaataatc cataaaaaaa agcaaaaaaa tttttgattt ttatcaaatt ctcttaaaaa
    12061 aatgtaaaaa atacaaaaaa accagtaatt gttacaacaa ttactggaca aaaactttga
    12121 ataatgttaa ctattttttt attttaactt tctccgcaat aatttctggg catgttccaa
    12181 ttcgtttata acgacttctt ctgtttcttt agctaatatt tcatctaata catcaagcca
    12241 tttttccggt tcttttgttc ctagacgtcc aatagcccaa gctgctgttc ctctgatgac
    12301 agggcggaca tctgttaaac attctgaaat ctcaggcaat gccgttcgat cacctagatt
    12361 agctaaagca atcaatgcgt ttctctgcaa aggtttcttc ccacgccatg aaccagctaa
    12421 atgcccaaat tgctgtttga agtctttatt agacatcgaa agcattggct ttaattttgg
    12481 atatatttcg tctacttttg gttccatctc ttcatgaaaa tgaaaatctt ttccttgatt
    12541 gtaagggcag actaactggc agatatcaca tccataaatg acatttcgca tttttttacg
    12601 atattcttcc ggcatcatcc cttttgtctg agtttgataa gacaaacatc gctgggcatt
    12661 cattcttccg tcaccaagca atgcttttgt cggacaagca gtcacgcagc gcatacagtc
    12721 accgcaacca aattctcctg gtttatcagg ctcaaataca atattagtaa cgatctcacc
    12781 aagatagaca aatgaaccga actcttcagt gatcaacaat ccgtttcgtc caataaatcc
    12841 taaaccagct cgctgtgcta cagcgacatc gatcagttct ccagtatcta cttgcggagc
    12901 tagacgccat tcctcttctt tttgccattt ttctgctctt gtttggatga attcgatcaa
    12961 tcggttcaaa cggtctctga gtatatcatg gtaatcgatc ccccaagaag cacgagcaaa
    13021 ctgcccgcgt ttttcatccc taggcacttc ttcatggatc ctagtaggat acgcaagtgc
    13081 aatggaaatg atcgatttcg gctgatcaaa tgtcatttct ggataaagac gctcatcgat
    13141 atttggatgt tcaaagccag aagtatggcc agctgctttt tgttcttcca acgattcttt
    13201 taatgaatca aaaggttccg ccgtggtgaa cccgattttg tcaattccta atcgtttgct
    13261 ttcttgtatg atttcttctt ttaatgtcat ggtcttcctc gcttgctgtt aacggctgta
    13321 taacccataa tatagttttt cttttttcag ctggacaaac tggatctgct gatcggcaat
    13381 cttcttttct tccatccatt tttttcttgc agcaaatact tcccaataat ttgttgaaat
    13441 taattttctt ttgttatcta atgcattaaa aaaattcaat gaaactggtt ttgtacttaa
    13501 cagttcatgg agccttacct cttcccatgg cgaaaactca gacagatcgc gggaatacaa
    13561 tacttcttca tagagatttt tttgctgtgt gtacgtgaat gtttgcggga ttttcccagg
    13621 cctagcaatt tctactagac gattataaaa catattcatt aatttgctgt tggcaataaa
    13681 ttgactttta taataggccg actggatctt tttgattact ccttctgctt tgatgactga
    13741 aaataatgca ggccatgctt tgacggttat ttcgtcgttt tgtttaacta atacgccagg
    13801 tgtgaagccg actggagaaa ggaacacaaa ataaccgata ccggtatttt catctaaagg
    13861 ctgaaattca tcaaacaacc atttgcctat gtacagctct tcatctggtt ctgttgtctc
    13921 attttgattt gctatgaata atttagctaa ttctgcttca agaaattgaa ggtcttgatc
    13981 cttgtcggta ttttcctgct ggctgatcaa tcgaaagact tcttctttaa tcgtatattt
    14041 atctttacgt gtaaaattca tagccacacc ttccttcctt actctaacta taacggaatt
    14101 tcttcaaaaa aggtatctgt acgacgaaaa acagcataat tttttttcat atctcttata
    14161 aaaaatgcaa ggtctgctcc tcaagctaga ccttgcactt ttcagctttc ttttgtttgt
    14221 gacaaacctt catggatcgt ttcaatattc catttcatca tctgatagta agaatctcct
    14281 tgttctcctt cttttgctat ggaatcagtg aacagtttat catagatttt caaacccgtt
    14341 tcttttgcta cccgttccat gcttcttgag tctacactgg tttccacgaa taaaaccggt
    14401 acttcttttg ctcttatctg atcaatgatc gctttcattt gatcaggcgt accttgactt
    14461 tctgtattga tctcccatat ataagctgct ggcaaatcat atgctcgtga aaaatacttg
    14521 aatgcccctt cacttgttac taataatttt ttgttctctt cgatagaagc aaaactttcc
    14581 ttcgcttgtg tatctaattc ttttagtttt tccacatatg cttctgcatt ttttttatag
    14641 agtgtcgcat tttctgcatc tttttcagag aatatacgtg cgatttcctc cacatatttt
    14701 attccgttag ataggtctag ccaagcatgg ggatctgctt ttgtatgttc ttcaccgcta
    14761 gttaaataaa gaggttctac atttttgcta actgcaaaat aatcttttcc ttcttctttt
    14821 ttagccgttt ccatcaagtt atcgaaccag ctgttacctg tttcaagatt caaaccgttg
    14881 tataaaataa catctgcatc gcttgccttt ttgatgtctt ctggtagtac ttcatattca
    14941 tgcggatctg ttccgactgg tacgatactg tggatatcta tttgatctgt tccgacctct
    15001 tttgccatgt ccgccaagat cgaattggta gccacgactg acaacttttc tttacttcct
    15061 tcatccgctt ggtcagaagc ctgctttccg caaccggcta ataccaagaa actaactgcc
    15121 aagattaaaa agagtgattt ttttgttttc atctatctgc ttcctttctg aaataaaatt
    15181 ccttttgttg gagaaaataa aaaagcaagg ataaaaaatg cggtacatgc taacacgatc
    15241 gtagctcctg atggccaatt gtaactataa ctgaaaaaca caccgacaac tgcacttagc
    15301 ataccgattc ctgcagctgt aatgatcatt ttcagtaaat ggttcgttag caagtaagcc
    15361 gtggcggctg gtgtgatcaa catggcaatc accaagattg ttcctactgt ctgtaaactg
    15421 actacagcca ctaatgtcaa aaagaacatc aaaagataat ggatcagcga tgtgttcagc
    15481 ccataagctt ttgccattgt cggatcgaat gatgtgatct tcaattcttt ataaaagaaa
    15541 aagacaaata tcaagacgat tccactaacg agtgctgtca gcaacaaatc gctttcccta
    15601 acagctaaga cattcccaaa taaaatatga tacaaatctg tcgcactctg ggcatatgaa
    15661 atcaagataa tccctaatgc aaaaaaggag ctaaagacaa ttccaatcgc tgtatcattt
    15721 ttcaatcggc tccgctgagt cacgaaacca atcactgctg ctgttagcat cccaaaagca
    15781 gtggctccaa agatgtaact aaaaccaaac atataagaaa tggctacacc tggaagaact
    15841 gcgtgagaga tagcgtctcc catcaaagac atccctcgta aaataataaa agaaccgatg
    15901 actccagaag caagtcctac aagcatggaa gtgaccaaag cgtattgcag gaattgataa
    15961 ttcattaatc catcaataaa ggattggatc attttttctc tcctcctttt actaaatgtc
    16021 tgatgatctc accataggcc aattgaaggt tcttcgatgt aaagacttct tgtactggac
    16081 cggaagcgat cagctgttta tttaaaagaa taacctcatc gaaatattct tctactttat
    16141 gaagatcatg atggacgatc aaaatcgttt ttcctgaatt cttcaattcc tgcaagatgt
    16201 caaagatctt tcgttcactt aacgcatcaa tccctacgaa tggttcatct aaaaagatcc
    16261 attctgctcc ttgggctaga gctcttgcaa taaaaactct ctgaagctgt ccacccgata
    16321 gttcgctgat ctgtcttttg gcatattctt ccatccctac ttttttcaaa gcttgtcttg
    16381 cacgttcttt ttcttttttc ccaggtcttt gtccaattcg taaagatgga tatgttccta
    16441 aaagtacaac gcctaataca tcaattggaa aggaaagatc caattcactt cgctgttcca
    16501 cgtaggctat ttttttacgg actgagttta ccgactgtcc agaaaaggtg actttaccag
    16561 tagtagctgg tatcaactct agcaaagatt tcataaatgt tgattttcct gcaccgtttg
    16621 gtccgataat accggtaatt ttttgtggat ttatcgtcaa gttgatagtt ttcaaaacag
    16681 ttttcccttg ataagcggct gaaaggtctt gaattacaat ggccgtacgt tccaaatctt
    16741 acactccttt ttaggtttac ctaacttttt atttaaatat agtttaacta aaatggaaag
    16801 cattttcaag tagaatgatg aaaaagtttt tttcgaattt tcctcgcatt ttatgaaaat
    16861 tttctgtaga atcacatcaa gagttcaata aggctaacct aataatgaaa aacatgtgtt
    16921 gaaacgtttg attatttttt cttgttgact caacaacgaa aggagttttt gtatggaaga
    16981 agaaaagtta actcgaagtc ttagtgcccg tcatatccaa atgatcgcac taggtggaac
    17041 gatcggtgtc ggtctgttta tgggtgcctc ctcaaccata cgttggacag ggccctcagt
    17101 gatgggagcc tatgcgattg caggattctt tttgtatttg attatgcgtg ctttaggcga
    17161 aatgctttac gttgatcctt cgactggttc ttttgcaaat tacgcgagcg agtacatcca
    17221 tcctgtggct ggctacttga ccgcttggag taatattttt caatatattg tcgtaggaat
    17281 aagtgaagtg attgctgtag gatcttatat gaattattgg tggcctgatt tgccggcaat
    17341 tattcccgga atcattgtcg tcttgtttct tatgctggca aatcttattt ctgtcaaggc
    17401 gtttggcgaa ttagaatttt ggttttcgat catcaaagtc ataacgatca ttttgatgat
    17461 cattgcaggt cttggggtaa tcttatttgg atttggtaat catggtcagg cagtagggat
    17521 ttcaaatctt tggaaaaatg gtggattctt cactggaggc gtcaaaggat ttttctttgc
    17581 attatcgatc gttgttgctt cctatcaagg aatcgaactg atcggaatga ctgctggtga
    17641 agcagagaat cctaaaaaga cgatcattga agctgtccag tccactatcg gtcgaatctt
    17701 gattttttat attggtgcta tttttatcat tgtgagtatc tatccgtgga atcaattgag
    17761 tgagattggt tcaccttttg tacaaacttt ttctaaagtc ggcattacat ttgctgcagg
    17821 actgattaat tttgtcgtga ttacagcggc attatcaggt tgcaattcag ggattttcag
    17881 cgcaagtcgt atggtctata cacttgccat aaatggaaag atgtctaaaa aatttctgaa
    17941 actatctcgt cacggtgcgc ctttttatcc tgtcgtagca atctcaggtg gaatcttaat
    18001 tggtctgatc ttaaattatc ttctaccaat gttcatcaac cagtcagaaa atattttcgt
    18061 tttcgtatac agctcaagta ttttgcctgg tatggttcct tgggtagtga ttttgatcag
    18121 cgagattcgc ttcaaaaaga agcacgcaga taaaatgcat gatcatcctt ttaaaatgcc
    18181 actatctcct tatacgaatt atgtgacact tgctttttta gctatcgtat tgattttcat
    18241 gtttatcaat cctgaaacga gaatctcatt gattattgga ctcgttttct tgctttacat
    18301 gacgatccat tactttgtac gcgaaaaaag aaccggaaaa taaaaaaaga acgaagcttc
    18361 accttagtgg aagcttcgtt cttttttatt gcttttatca attcaatttt agcgtctcac
    18421 cgggagtcag gaaataattc tccatgctca tcccattcag cgacaagatc gtttctactg
    18481 gtacgccagt tcgttcggct atttgatttg gaccttctcc ttccaaaact acggttgtcg
    18541 cagcctgtga agtcgcgctg ttggtattag tttcagagga atctgttgtg tcttgtgttg
    18601 ttcgagcttg catttcagaa gcttctaccg actctgctgt ctcagaatca gaagatgttg
    18661 tgtcttgtgt gcttgttgac tctgcttgaa gagaggcgct agtctgcaaa taaatatcgg
    18721 aaatcttagc gacatgattc gtataattag ccgataattg attgatcaaa gtatctaagt
    18781 ctacaccgtc tttattttct gctgcctgat acgctgcacg taattttctg atcccttcgt
    18841 cggagattgc ttgaagctca cgtgcttttt ggttagcaac tgctgataaa ccagctattc
    18901 cctcttgatt tccctgaatt tctgtctggt attcttcaat caatgcaggc gtcttttctt
    18961 cgatcttttt cgaataatca tttacgattg cttgaagttg ctctggcaaa ccatttgctg
    19021 ttttttcttc agaagtttgt gtctcttttg ttttagttga tgactcactt gtttgtttcg
    19081 tctctgtttt ctttgtcgtt gtttccgtta tggtcgtact tttcgcttct gacgaatttt
    19141 ttccatgaaa atatagaccc gcaactcctc ctgctatgat caaaaacacg gcaactgctg
    19201 ccccaatcat gatcatccgc ttacgagctg cctgtttttg ctgtcttctt cgggtcatcc
    19261 gaacattttc tccccactca ttttcatcat acattttttt caccaccaat ttttatgtat
    19321 aatagcgtta tcaaacgctt catattctta ttgtagcaaa taattgtcgc tgtgcacatt
    19381 tttatctttg ttatttataa gaaaatttca ccagttgaga tgattccaag attgtccgtc
    19441 gatttctcct tgcagctgca ttgttcgttc gtctatctca gccatcagtt tctttatttc
    19501 atataagagc gcacggtctt gatattctga tttttcgctt aattcatcta attgttggat
    19561 cagccaatca tttgtcataa tgaagttcct ttcatttctt gcagtgtaaa ggtcaagtct
    19621 tcgatctgct cttttgcctc agtcagtgct tctacgatat atgcttgttt ttcagattct
    19681 tcttcctgca ttttctgatt ttcctgtact ctttgaataa accagtcaat ccgctgagtc
    19741 agcgatggtt tgccttccaa tgagagatac tgctgataag caattagctt ctcccaacgt
    19801 ttttcctggg ataaatacat aaattgtgct aaaacgaatg gcggaagata ggtcatatgg
    19861 aatttatttg cgattcgctg atacggcctc aaaaattcgg aaatcgtata ttcttctttt
    19921 cctccagctt ggtattcttt ttccgccaca cctgttgtca atacgattcc tagttctttc
    19981 ccttttaata catgtgctgc tcgttccaag acattatctt gccaaatttt taaatgagca
    20041 ggagaactat accagtaaaa cggaaactgg aaaatgatcc gatcatgctg ggccaagagc
    20101 tgtagttcag tctctatatc tatttttccg tctggataac ttgattccaa atgatgccaa
    20161 gtagctggta aaccagcaga agcttctttg aaaaattgct gatttaaaga ttcttcgatc
    20221 gttggatggc taatgatgat aagtgtacgc atcccttttc ctccttttta gtcttcgctt
    20281 tacagtgtag cataaccgcc acaagtttct tttgccgatg cttgtcattt taaccagact
    20341 aatcaggttg tccaagaaac tgcggtttgt ttttccaact gttttttcaa ggtatccaaa
    20401 gcaatattct taaaattttc gtttttcata cgtttacgat agttcgtagc cgtttctccg
    20461 gtactctttt tgaacattcg gctaaatacg actagactat tgaaccctac ttgataggca
    20521 atactttcca cggattcatc cgtcagagaa agaagaaacg cccctcgatt gatccgtacc
    20581 ccgatcaaat attccttgat actggtaccc atatgatttt tgaatactcg cgacaaatgg
    20641 ctccgattga ccgctaaatg ctgggcgatt tcctcaacag acaatttttc agcataatgt
    20701 gcctcgatgt atttgactgt ttcaacagct aaagaggaat gaaccttttg tacaccaccg
    20761 ctgacctttc cgccatcttt taaaaggact gataaaaaac ggtaggttaa tgctgttaac
    20821 tgtaattcat tttccaaggt ttcttccgta tctttcatgc attctaaaat aatgtcgata
    20881 tatttttgta tgtcagaaga gaccatcgtg tattggtctt ctctgaaaag cgactgccga
    20941 atgatttctt ctgcagcact cccaccaaat gaaagccagc aatacatcca cggatcttct
    21001 ccatctgata agtaaaacgt cgagtctcct ggacgaatca agaacagatc accttttttc
    21061 aattggaatt gttgattttt cacatgataa gatcctttgc cttccatcac gacatgcagg
    21121 atgtaatcat tgcgaatagc aggaccaaaa gaatgaaacg gcatcgtttt agagaaacca
    21181 caaaaatcaa aatacaattc tttttctact tgttgatgtt ccttttttaa aaaaatcgat
    21241 tgctccacgt ctatccctcc ttactccttt agtataatgc aacaaaaagt taaataaaag
    21301 tcacagaaat atattaaaaa ggaagcgatt tcttgttaag tttaagtcat taaaagatct
    21361 ttgttattcg ttgtaaccgg tttacttagt attatatatt gaacaggaga ggaatacaga
    21421 ttatgaaaag caaaatgatc caacgtgtgt cctacgcttt aggcgctttt ggacacgaca
    21481 cattctatgt tacattaagc acttatttta tggtattcgt gacaagccag atgttcgcag
    21541 gaacagataa ggcaacgaat gcaaaaatga ttgctacagt tacaagctta gttgtaggta
    21601 tccgcttagt tgaaattatt tttgatcctt tgattggtgg catcatcgat aatactcgta
    21661 caaaatacgg taaattcaaa ccatggttag ttatcggcgg cgttgttagt tccgtactct
    21721 tggtagcaat ctttaccaat ttctttggct tagctactca taatacgact ttatttatca
    21781 tattatttgc aatcgttttc atcttattgg atagttttta ttcatttaag gatatcgctt
    21841 tttggtcaat gattccagct ttgagtacgg aaacaaagga acgtgaaaaa tttgctacgt
    21901 ttgctcgttt cggctcttct ctaggtgcaa atggtactac tctagtcgtt gtccctatcg
    21961 ttactttctt tacctatctt gttactcata aacatattga aggcgcttca ggctggttct
    22021 ggttcggtgt gattgctgct gttatttcag gcggtactgc gattatcact gccttgggca
    22081 caaaagaagt cgatactgcg atccgtcagc aaaatgaaaa agctgggatc aaagatgtgt
    22141 tcaaagcagt tgctcaaaat gatcagttga tgtggcttgc ccttgcttat atcgcttatg
    22201 cgattgccaa tgcagcaaca actgccgtat tattctactt cttcaaatat gtacttggcc
    22261 aaccaactga attttggatc gttggtctag ttgctgcctt acttggttta gtagctgttc
    22321 cactattccc aatgcttaca cgcgtcatct ctcgtcgcta cgtttacctt ggagggatcg
    22381 caatgatgat ttcttcttac tttttcttta cgatcgcagg ttcaagtttg ccaatcgtta
    22441 tcatcgggct agtattgttc tacttccctc aacaattgat tttcttatcc gctttgatga
    22501 cgatcacaga ctctgttgaa tatggtcaat ggaaaaatgg tatccgtaac gaagctgtta
    22561 ctctatcaat tcgcccttta ctagataaaa tcgctggtgc tttgtctaac gggatcgtag
    22621 ggtttgtcgc tatcgcttct gggatgactg gtaatgctac tgctgctgat atcactgctc
    22681 atggcgtaac gactttcaaa gcttatgcat tctatgctcc tgctgctttg atgattttct
    22741 ccgctattat ttttgcttgg aagattacat tgacagagaa aaaacatgca caaatcgttg
    22801 agaaattgga aaacaaattg gctccagctg atacaatgga agattaactt cacgatgcag
    22861 tagttccttc ccattaagaa aggatcttga aaatgaagat tacagaaaaa gattttggcc
    22921 aaggttatca cttgataacc ttggagaaca aaaacaagct agcactttct atctctgatt
    22981 taggtgctag aatcgtcagt ttaaaatcaa atgatcgaga attagttttg ggatttgaca
    23041 ccgctgaaga atatatcgaa aaagatccgt atatcggtgc ttctatcggt cgcaccgcag
    23101 ggagaatcga aaatggccgc ttttctctca atggaaaaac ttaccaatta gccacagatc
    23161 caaaaactgg acataatctg catggtggtg ctcctggatt tgaattaaaa aaatggtctt
    23221 atgtcatttt gaatggcgaa aatgaagctt ctgtcatttt tactactaca agtcccgacg
    23281 gagaacatgg ttttccaggt acaatggatg tggaaatccg ctatactttg acaaaagata
    23341 atatctggcg agtgacctct cgcggcacca gtgaccaaga tactttattc aatccgacaa
    23401 atcatgttta cttcaacttg acgggagatg cttctcagtc aatcgatcaa catgagcttt
    23461 ggctcaatag cgaagcttat gctcctttgc gaacagatag tattcctata ggggtaaaag
    23521 aaaatgctgc aggaagtgct ttcgattttc aaatacctaa aaaattagcg tccgtttttg
    23581 ctagtgattt ggatcaaaaa aatcttgttg acggaatcga tcatccattt ttcttaaaag
    23641 aaaccggctt gggtaaagaa gccgctcgct tgactagccc cgataaaaga atccaagtag
    23701 atattgctac agatgcttct agtgtggtta ttttcactgc aaatttcggc acagaaacac
    23761 cagaaatgcg caaccgaaaa ctggcacatc acggtggcat cacatttgag actcaaacag
    23821 ctccaggtgc agagcgcttc tcagcatttg gttcgattca tttaaaagcc ggatcagtat
    23881 ttgaaacagt aacagaattc aaaatcaaaa caagaaagga ataactcatc atggaaatca
    23941 aaagaagctt aaacacaaaa tttgccgaaa cattcggacc taaaaaaact acacaatact
    24001 tctcaccagg acgcatcaat ttgattggtg aacacacaga ttacaatgga ggccatgtct
    24061 tccccgcttc catcacttat ggaacgcaag gtgtagcagc acctcgagac gataataaaa
    24121 tattagttta ttccacaaac tttgacgata tcggtgtcat tagttttact ttagatgagc
    24181 tagaatatga taaaaaagca aactgggcaa attacgtaaa aggaatggtc cttaaattaa
    24241 aagaagctgg acactcaatc gatcatgggt ttgagttatt agtggaagga acaatcccta
    24301 atggtgcagg actttccagt agcgcttcat tagaattatt agtaggtgtt gtattagaag
    24361 atctgtttga tttaaaaaca gatcgcttgg aactagtcca aacaggtaaa aaagtagaaa
    24421 atgaattcat cggtgtcaat tcaggtatca tggatcagtt tgccatcggc ttcggtgagc
    24481 tagatcatgc aatcttatta gatacaaata cgctgaaata cgaaatggtt ccagtgaaac
    24541 tagatggtta tgcaatcgtc atcatgaata cgaataagcg gcgggaattg gcagattcaa
    24601 aatacaatga acgccgtagc gaatgcgaag aagccttggc acgtttgcaa aaaactttgc
    24661 ctatctcttc tttaggagac ttagatgaag aagaatttga atcaactatt gaccaaatcg
    24721 gcgatgatac attgatacgc agagctcgcc atgccgtttc tgaaaatcag cggactttga
    24781 aggcaaaagc tgaattagaa gctgggaact tagaagcatt tggtcaactg ttaaatgatt
    24841 ctcaccactc tcttcgctat gattatgaag taactgggat cgaattagat acactagtag
    24901 atgctgctca aaaacaagaa ggtgtcttag gtgcacgaat gacaggagct ggatttggtg
    24961 gctgtgcgat tgctcttgtc aaagaagaaa atatacctgc cttcaaaaac aatgtctatg
    25021 atgaatacat gaaagttgtc ggctatgctc cggaatttta tgttgcacac attggaaatg
    25081 ggacaacaaa gatcgatcca gaagctgttc atgtttgata aaaaaatcaa cgatctcagc
    25141 taaaaacaaa tagataaaaa taacctctca gcaatttcca aatgtgagcc ttttgctcgt
    25201 atttgaaaat tgctgttttt tattgacacg gaaagagaac gtgtgttcta attgtgttga
    25261 agaggtgatt gttatgtggg cgaatcaaaa tccggtattt gtttatgaaa aagaaccttc
    25321 aagagatatt ttatgtattg actgcaaatc tttttacgcc tccgtagagt gcgttgaaaa
    25381 ggatcttgat ccccttacaa ctaaattagt agtcatgtcc tatccgtccg attcgacaga
    25441 aacacggggt agcggattaa tcttggcttc cagtcctgca gcaaaaaaag cttacggcat
    25501 caccaatatc agccgtgcca gagatcttcc cttcccctat ccaagtgatt tatatattgt
    25561 tgccccaagg atggcctatt atatggaaaa gaacagagaa atcaattcta tctataaaaa
    25621 atatgcagac gaagcgaacc atcatgtcta ttccgtcgat gaaagcttcc ttgatgtaac
    25681 aaatagcatg gcactctttg gtaaaacaaa tgcgtacgac atggctaaac tgatccataa
    25741 agatgtgttt gagcagaccg gaatctacac gactatagga attggcgata acccgctact
    25801 tgctaaacta gccttagaca atggctcaaa agacggaaag caaatgtttg ctgaatggcg
    25861 ttatgaagat gtaccagaaa agttatggaa aatctcagat atcactgact tttggggaat
    25921 cggcaaacga acagcaaaac gtctgaaaca aatgggtata cattctgttt atgatttagc
    25981 tcacgcggat tattatcact tgaaaagcaa gctaggggtc atcggtactc agctttatgc
    26041 ccatgcttgg ggcatcgatc gaagtttttt gggacaagaa tatacaccaa aatcaaaaag
    26101 tatcggaaat agtcaaatcc tagatagaga ttatacaaga cgagaagaaa tcgagatcgt
    26161 tatcaaagaa atggccgacc aagtcggcac tcgcttgaga cgatctgcag caaaaacaca
    26221 agtagtcagc ttatgggtag gatactcttt aggctatctt gatccaaatg gcaagaccgg
    26281 gtttcaccag caaatgaaaa tcgaagcgac aaatgctagc agagatcttg cagatgcttt
    26341 attgaccatt ttccaccagc attaccacta ccaggatatt cggcgtgtag gcgtgaactg
    26401 ttccgacctc gtatacgcga caggtttaca gcttaacctt tttgaagatc ctgaaaagca
    26461 gataaatgaa gcgaaagttg attttgttgt cgatacgatt agaaaaaaat acggctttca
    26521 atcaatcatc catgcgcata gtctcttgca aggaggacgc gccatcgcac gtagttctct
    26581 cgttggcggg catgcaggtg gtatgagtgg attagaaggt ggaaaagatg agacggacag
    26641 caaaaaaatt cattgactat aacgagtatc gagatcggcc atttggatta aaatggggga
    26701 ctgcttttgc gatggaagaa ctagtcaacg gtatcaaaaa aatgaagaag aagcacgcaa
    26761 acagcctctt cctaaacaac aaatgacatt gcaagaaatc gatagtatac tgacagaagc
    26821 ctttttatct cgtaaagcag taatcattca gctcaattta aaagatgaat ttggaagact
    26881 cttggatgag gtcgaagggc acttcattgg tgaagcctat gaagattact ttatgattga
    26941 ccatcagccg atattgtggg aagacgtacg gtatattgag ttaaaggaaa caaaaaaatg
    27001 gtttgtgaca tgagaggttg tgacagaagt gatcaacttc aaaaaataag aaaggatttt
    27061 tcgaaaattg cttcttaaat ttttgtggaa ttttggctta tttttcgaag aagcaacttc
    27121 tggaacaacg tatattagat atcagaggtt gtgaaaaagt cgcttagttt tgagtaacaa
    27181 gccaaggcgc tgatttcaca acctctgtct atctatccat ctattcggta agattcatgc
    27241 atgacgtctt ctttatttag tatgtagagc cctttttctt ctgaccattc aacataaatc
    27301 agctgtttta catctgcata gccttctttc ttcaagttct ccataagcca tgcttcagtc
    27361 aattgattgg actgaagcac tttatgctga atttgccctt catctacaag caggatcgat
    27421 aaagctttgt cttcatctga ttttttcaat acactcattt gaccggcatt ctctaggata
    27481 acatgtttgg cattttcaag aaaaaagcat tcttgctgtc ttaagattgc tcgcaactgc
    27541 tccatctcga tatggttatt tgtcaactct gtcatgttga tgacgccgtc tttgattaga
    27601 acagatggct cgcctttgac ccaacgattg accttttcat ttttttggac gatacgttcg
    27661 ataccataaa tcattactgc ccaaagcgca atggcaaata ggacatgccc tacatggaca
    27721 ttgtcatcat aggttgattc ttctaagatc ccacctaaaa caagcgtata gactaaatca
    27781 aacggtgtga tttctgacat ggatttcttc ccaagcaaac gtaccactaa taataaaccg
    27841 atcaacccga acaccagttt cattgtaatt gaaaaaatca ttttttctcc ccctaactat
    27901 ctgttaatta aattatatat gctttctcac gaacacgcat ggaatataca aggaagaaat
    27961 gtatcctttt actaaaaaaa ggactgtgac actgtagtga cccccaaaag ttagactttt
    28021 ttggagtgga gaaatccact cctttttttt atgctctaaa atgatatttt tacacaaccg
    28081 ttacggttgt tttacgaaat tggacgagac tttgccagtt taatttctgt tttatccgtt
    28141 cattattgta gtaatagata tagctatcga tggctgattt taattcctcg aaacagttat
    28201 aaactttccc gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc ataagtgaat
    28261 tatctaagca gttccctttc cgagacatgc tttgaaagat tttgtactcc ttcaatttgt
    28321 ttctataagc tttcatttga tacgcccaac cttgatctga gtgaaacgta cgacgatagg
    28381 gacaatcgtt agtggtctgg atagcttcct ccaaaccttc cattaccgct aatgcattgg
    28441 gcttttcaga gatacgatag gataaaatct cactattgaa catatccata aaaggatcta
    28501 gatacaattt cttttgacgg ataatgcctt tggcatccac ttcatggtat ttaaattcag
    28561 ttgtatccgt cgtgattttc tgatggcaga tactggtgta gaaacgacga tggat
//
LOCUS       DAZKSR010000031        27826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000031 DAZKSR010000000
VERSION     DAZKSR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.031"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(276..1220)
                     /locus_tag="ACG8XG_001188"
     CDS             complement(276..1220)
                     /locus_tag="ACG8XG_001188"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0773115.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1516..2460)
                     /locus_tag="ACG8XG_001189"
     CDS             complement(1516..2460)
                     /locus_tag="ACG8XG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0773116.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2681..3895
                     /locus_tag="ACG8XG_001190"
     CDS             2681..3895
                     /locus_tag="ACG8XG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0773117.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3917..5932
                     /locus_tag="ACG8XG_001191"
     CDS             3917..5932
                     /locus_tag="ACG8XG_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0773118.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6015..7388)
                     /locus_tag="ACG8XG_001192"
     CDS             complement(6015..7388)
                     /locus_tag="ACG8XG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0773119.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7414..7698)
                     /locus_tag="ACG8XG_001193"
     CDS             complement(7414..7698)
                     /locus_tag="ACG8XG_001193"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0773120.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7700..9715)
                     /locus_tag="ACG8XG_001194"
     CDS             complement(7700..9715)
                     /locus_tag="ACG8XG_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0773121.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9894..10199)
                     /locus_tag="ACG8XG_001195"
     CDS             complement(9894..10199)
                     /locus_tag="ACG8XG_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0773122.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10288..11319)
                     /locus_tag="ACG8XG_001196"
     CDS             complement(10288..11319)
                     /locus_tag="ACG8XG_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0773123.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11526..12401
                     /locus_tag="ACG8XG_001197"
     CDS             11526..12401
                     /locus_tag="ACG8XG_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0773124.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12553..13395)
                     /locus_tag="ACG8XG_001198"
     CDS             complement(12553..13395)
                     /locus_tag="ACG8XG_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0773125.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13487..15364)
                     /locus_tag="ACG8XG_001199"
     CDS             complement(13487..15364)
                     /locus_tag="ACG8XG_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0773126.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15361..16782)
                     /locus_tag="ACG8XG_001200"
     CDS             complement(15361..16782)
                     /locus_tag="ACG8XG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0773127.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16785..20174)
                     /locus_tag="ACG8XG_001201"
     CDS             complement(16785..20174)
                     /locus_tag="ACG8XG_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0773128.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20431..21822
                     /locus_tag="ACG8XG_001202"
     CDS             20431..21822
                     /locus_tag="ACG8XG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773129.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21895..22542)
                     /locus_tag="ACG8XG_001203"
     CDS             complement(21895..22542)
                     /locus_tag="ACG8XG_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0773130.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22550..23236)
                     /locus_tag="ACG8XG_001204"
     CDS             complement(22550..23236)
                     /locus_tag="ACG8XG_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773131.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23242..24315)
                     /locus_tag="ACG8XG_001205"
     CDS             complement(23242..24315)
                     /locus_tag="ACG8XG_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773132.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24537..25211
                     /locus_tag="ACG8XG_001206"
     CDS             24537..25211
                     /locus_tag="ACG8XG_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0773133.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25216..26556
                     /locus_tag="ACG8XG_001207"
     CDS             25216..26556
                     /locus_tag="ACG8XG_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0773134.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26705..27691)
                     /locus_tag="ACG8XG_001208"
     CDS             complement(26705..27691)
                     /locus_tag="ACG8XG_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0773135.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8120 a         5252 c         5047 g         9407 t
ORIGIN      
        1 cagttaatca aatatgaacg aattagatat gaatcattgt atttgccgtt gcggaaggat
       61 tttttaatta accacagata aacattgata acgattcgct tattgtaatc aacagattaa
      121 atttcatatg ttcatatgta cggatacatt ttcggaccga ttaattttaa ccattctatt
      181 tatttgctta cctattagat atcaataaaa aactgcttaa cagctatttc agctgttaag
      241 cagttttagg atttttagtt catattcatt gtttcttata atacagtgtt caatacttct
      301 cttaaagcac ttgttgattt gctgaacatt gctttttctt cttcgttgat tgataattca
      361 acaacttcac gaacaccatt ttcgtcaacg attgatggca cacctgtaaa gatatctttt
      421 tcgtcgtatt caccagttaa ataagcagat acaggcaata cagcttgttc gttattcaag
      481 atagctttta cgatacgtgc agtactcata ccaataccat agtaagttgc ttttttacga
      541 tcaatgatct cataagcagc attacggact ttatcagcaa tcacatctaa ttcatcttgg
      601 gcaatacgat ggtctttttc tacaatttca aacactggtt taccaccgac agttgtatgt
      661 gaccatgcag caacttctga atcaccatgt tcacctaaga tatagccgtg gacgctgcgt
      721 gggtcaactt ttaatttcaa tgcgatttct ttacggaaac gagttgtatc caatgttgtt
      781 ccagtaccga taacacgtga tgttggcaga ccagattcgt tccaagcgat atatgtcaaa
      841 atatctactg ggtttgaagc aacaacgata ataccgtcga atcctgatcc catgatttct
      901 tttacgattt ggcgcatgat tgacgcattt gttttaacta aatctaaacg agattgacca
      961 ggtttttggt tgataccagc tgtgataaca acgatatcag catctttaca ttcttcatag
     1021 ccacctgacc atacagccac attttcatcg ccccaagcca taccatctaa aaggtccaat
     1081 gcttcccctt ctgctttttc ttgattcaca tcgatcaata ctaattcatt agagattcct
     1141 tggttgatca ttgcataagc gatacttgtt ccaacaaaac ctgtaccgac gattactact
     1201 ttacgacttg tttttttcat gaataaacag ctccttttca taactatttg ttactttcat
     1261 gaaaagtata gtcttgttta ttcattttat caacatgtta attataatag ttgttattat
     1321 aatttttgat ggaaacgaat tcctgaatac gcataacaga taaatgacaa atgtttacac
     1381 ctatatcccc tgataataca accaaaagtt tagaactgaa aataaatcca aaaaaattcg
     1441 ctgcaacgat ataataagac agcaatcgca cctaaaacaa ttttccttca tatgctttag
     1501 tgaaagattt tcttcttaat gtttatttct ccattcattg ggagtacatg ttgaatactt
     1561 cttgaataat ttgaataaag aagtcgtttg tttatatccg atacgattag cgatttcttc
     1621 caacgaaaaa ctcgtttctt gaatcagttc caacgcaaag tcgaattttt tctttagaac
     1681 cagttcttga aaggaagcat caaaatacat tttcaattta ttactaagat agaccggatt
     1741 gtagccaaaa tgagctgctg tttcttttaa cgtacaagaa gtaaaatgct tatcgatata
     1801 atctaatact ctaagcatct cattttctgc gcatccatct gctgatgaaa agctttgttg
     1861 ttctaataaa tgagtcaatt cgatcagtaa tagtgatgta tagagattta acgaacggat
     1921 cttatgactg tatgtatcaa aataaatcaa cagcatattt tcaatcaaat gcgtcaaacg
     1981 acttgttttt ccgccgcgaa aatgaatgaa ttgtgtatgt tccccttttt cttttgatgc
     2041 atttatcaaa aaagaagaaa gtagattatt tgtgcttgcc aaatgattca atatctctgt
     2101 agataaggtt tcttctttta ataagatgtt gattaaaata tcctcttgcc ctgtgtattc
     2161 gattctttga gtagcttctt tatcaatgat caccacgtca ctttcttcca tctctatcct
     2221 ttctccatta ataatttgta aacatgtacc gctatacata taattgattt caacaaaatc
     2281 atgtgtatgc tcgggaactt ccgcatatcg acggtgttta ctgatatgaa tatttccctg
     2341 ttgaaataga tcccctcggt ggatttttgg cggtgtctga agatggtaag ctaagtttaa
     2401 atcattgata ttcattcctt gttcacgctg cagagtttcg attgattctt cgttacgcaa
     2461 aaaaaatgtc taattcagct agttccaatt gactccctcc tttttgtaag cggtttcttt
     2521 cgcttattag ttcacgctgt actgctatta tatgcaaagc tatttttggt ttcataaaga
     2581 ctttctatgg ttgggacatg aacttcctgt gattccgcta ttgagagaat aaccacttca
     2641 tactatattt ttgataagcg cttacaaaaa tgaaaggaac atgcttatga ataaaaagat
     2701 gatgaaattt tctctgctgt cagtctctct tattttgact tctgctggtg cagtcacgtc
     2761 tgcaattccc agtatgtcag ctacttatgc cacgcgttct ttatctgctg ttgaattatt
     2821 aacgactgct ccctctttta tggtcactat ttttgttttg attagtagtt ttattgctag
     2881 gaaaatcgga caaaaacaaa ctgtgatcat cggattagtg attgcatcga tttgtggtat
     2941 cctccccctc ttctcacaaa gttacagtat tgtcttagct tcaagagctg gactaggtat
     3001 tggctttgga ttgatcaatt ctttggcagt cagcatgatt gctgcctttt atcacggaga
     3061 tgaacgagcc tctatgattg gttttcaaag tgcatttcaa ggatttggtg cagctttaat
     3121 gacatttgtt gcaggacagc tagtacagtt cggttggcaa tatacctttt tagtctacgc
     3181 catcagctta cctattcttg tcttgtttgt tttgtttgtt cctacacctg aaacaactga
     3241 acttcaatcg catggcacag ccccaacaag acaagtaatc aaaacgaatc ctaaaatgat
     3301 tagttatggt atttttctct tgctggtcat cgtggtatat aatgctgtat cgattaaatt
     3361 agccacagtc ctgacgactt attctctagg aaacgaaact aatgcagcga atatcttaac
     3421 aatcatgcaa ttggcaagta tggctgccgg agtttgtttt ggctggcttc agacaaaagc
     3481 acatcgctat atgcttacac tttctttact actgatggga atcggctttg tattgattgc
     3541 cactgtgata aacctgtatg tgattgggtt tggtgcgatc ctaacaggaa tttctttttc
     3601 gttattcatt ccttacttat tcaaccaagt aagtatccaa gcgcccaagt acgcggagag
     3661 tttcaatact tcaatcttat tggttggcgc taatttaggt actttcattg caccatatgg
     3721 tttgctcttt ctctcttttg cttctcttgg gagtaaaaca cttgctgttt tcgtcaatgg
     3781 cagtatcttg ctgttgatta gcgcgcttat tagccttcta gtagtacaca ggacgaatag
     3841 aaagacagtg ataagagtag atgaagaaat aaaagaacaa ttgacaacaa aataaatttg
     3901 atggaggtcg tgtaaaatga caaatgaaaa acataaagat agatggcttt ggtatcctgg
     3961 agactttgag atccgccatg gactgctaca aaatttccaa cgggaagaac gaggttttga
     4021 ctggccagcg tactggtaca tggatgattg ccatcggaac gtcaagttta agagatatta
     4081 ctttcttgat caaccttcca tgtttaaagt gacgatccaa ggagttgggt atgtagaaat
     4141 taatggacaa aaacatccat gtggaaaatg gctgacttgt ccagctggta aagcaaaaat
     4201 tcgtatcttt gtaggacata catcagggct accagcgatg tttatcgagg gagacgaagt
     4261 aaaatcagat attggctgga cagcaagtaa ttttattgaa gaatatccag ctggctggag
     4321 tccgctttat gtcgatatcg caaaagatcc aaatcaaatc tattatcaaa aagaatgcgt
     4381 ccatccagtc actgaaacgg agattggcgg aggaatctta ttcgattttg ggcgagcagt
     4441 gaatggctgt gtagaactaa tatctctgga tgaccatatc gaatctatca ctctatgtta
     4501 tggagagtcc gatgtagaag ctatggatat cgattattgc tactataagc aaataggaat
     4561 gacaagaaaa atgaaagctc gtaaacgcgc ttttcgctat ttgtttatac cagaggtttc
     4621 tactggaatg gttcaacttc aagtttttca tgaattttta tctctttcga aacaagctgc
     4681 tttttcaagc gattcttctt tactgaatca gatctggtca gtatcagaag aagccttcaa
     4741 gttatgtagt gatttgtttt ttatcgacgg tatcaaacga gatcgctgga tttggtctgg
     4801 agatgcgtat caatcattca tgatcaatca atatcttttt tttgacgagg aaatcaataa
     4861 acgaacgata ttagcattaa gaggacaaaa tgaaatcaag cagcatatga atactatcgt
     4921 tgactattcg atcttgtggt tgattggtat cgaaaaccac tatatgatga cgaaagacta
     4981 tgaattttta cgcatcgtct accctaagat gcaatcatta atgagatact tgatggagca
     5041 aacgaatgaa ttagggttta tttatgggag agcaaaagat tggatattta ttgattggtc
     5101 tgaaatggat aaagaaggaa cgcttgctgc agaacaagtc ttattactga aagcctatca
     5161 ttccatgatc acttgcggag atatcttagc tgaaacatat ggagagaacg aaaattatca
     5221 tactcatctc tatcaagaaa aatatcaact attgaaacaa aacctatttg actacttttg
     5281 ggatgaagaa cttggcgctt ttatcgattg ctatgaatct gggagaagaa atgtgacgcg
     5341 tcatgcgaat attttcgctg ttttattcga ttttgctaac aaagaccaac agcaatcgat
     5401 cttaaagaat gtcttattga atgacactat cacacagatt actactccat atttcaaatt
     5461 ctatgaacaa gatgctttat gcaaactcgg acagacgaat atcgtctacc aaacgattct
     5521 tgattattgg ggagggatgt tatctcatga tgcggtaact ttctgggaag aatacgaccc
     5581 tactcaaaca ggaaatgaaa agtacagtat gtatggagat ccttatggaa aaagcttatg
     5641 ccatgcatgg ggagcaagcc caatctatct tttaggacgc tattttatcg gtttgtatcc
     5701 aacaaagcca ggttatgaag aatttatgat acaacctaat ctagattcat ttgaaacact
     5761 gaattgtacg ctacctatca aagatggaaa gatttcgctt gaaaaaacaa aagataagat
     5821 cgtgattact tctgatagaa caggcggaac agtgaaaatg aatggtaaaa gttatcctct
     5881 tctagcaaaa cagccacttg tcttaacaag ctattcaaaa ataaatgcat aaaacaaaaa
     5941 actgcttgcg acatatcaga agactttaaa tcttcttctg atattgtcca agcaattttt
     6001 attttgaagt ttgcttaatt ctgttcttta gaaacaatgt ttgttctttt tccggcaaaa
     6061 agaaagctag ataatacgac aagtaaaaag aaagcgatga ttccaactgt caaggctatt
     6121 tgaccgtatt ctcctagttt cccaagtaca agtcctggta ctagataatc agtatcggca
     6181 aatgtagaag aagcgatttt aagatctcct aatactggaa ggataaataa tggcagccaa
     6241 ctaatcaata agctattgaa agcagaacct aataccgccc ctcttctacc gccagtcgag
     6301 ttgccgaaga caccagaagc agctccgcag aagaaatgtc ctactacccc aggaatgata
     6361 actgtagttt gcaagcctat catagcaaac atggatagtg ttcctacaaa gaagctaaca
     6421 aaaaagccaa ttaatacagc attaggtgca tatggaaaaa cgataggaac atctaatgct
     6481 ggtttagaat cgggtacgag ttttttagca atcccttgga atgctggaac gatttctgcc
     6541 aacaccattc gaaccccttg aaggacgata acaaacccag ctgtgaatgt tccagcttgg
     6601 atcagtgcaa agattacagc gttgtctcca ttgcttagtt ctctttcaac aaatccagga
     6661 ccagctaaca aagcaagtgc gacatatacg atcgacatca agagcatgat cgatacagta
     6721 gaatctcgca aaaagctgaa acttttagga atctttatgt cctctgtaga acggtctttg
     6781 tttccaaaaa actttccgat ccatccacta gaagcataac cgatatttcc ggtatgccct
     6841 aacgcaactt gattgtttcc agtgatgttc ttcataaacg gttgacaaag agcaggcgta
     6901 atagttaata gtgttccttc gaagaccccg cctaataaga tcatccacaa atttgcagta
     6961 aaccccgcgc ttactaaaat gactgctgtt agacaagaaa cataaagcat tgcttgacct
     7021 gtaagaaaaa tgaatttata tcttgtgaat ctagcaagta aaatattcac gatcatccct
     7081 actagcatga tcaaagcagt aggtgtaccg tattgttgca aagcaatcgc cactactgct
     7141 tcattgctag gaactactcc ttgagtatgc agagctgttt ggaacatcga agcaaaaggg
     7201 gttagtgaat tactgattaa accagcaccg ccgatcagta ctaggaatcc gacaaatgtt
     7261 ttgatagttc cttggatcac ttctgaaaat ttcttcttct gaagaacgag accaagcagt
     7321 gcaatcaaag aaacgaggat ggagggctga ctgacgattg acacaaacaa attcaacaat
     7381 ttatccattg atagttcctc ccttgaatca tttttattct tttgacaaga tcttcgttac
     7441 tttttctttt acttcttcgc tgtcaatgat cgattgcaaa ggtacgagct tttcatcagg
     7501 tatagaagtt agcgtatctt tcaaagaatg ctcaatgaag aaataatcag ccatatcgct
     7561 agatacactt ccaacatccg tatgacttac ttcaaactcg tccacatctg ctccaagatc
     7621 tgcaagtgct tgtttgatgt tcatctctac cataaaactt gttcctaatc cactttgaca
     7681 tactgcaaaa attttcatat cattcgtctc cttttcttat gatcgctaat atttcttctt
     7741 ttgttgatgc gtgtaataaa tcttgaagat tttgtttatt agaaagtagc tttgttaaat
     7801 ttgccaaagc ccgaagatgt gcctcattat cgatagcagc taaacagatg aacaaactaa
     7861 tgggatgggc tggattatca agcagatcca caggttgttg aagtttcagc aaagacatcc
     7921 ctacttcgtt tacgccatct tctggacgag catgaggtaa tgcgatgtga tcgccaatat
     7981 ggataaacgc accatactgc tccacgcgct cgatcatagc tttgatataa ctttcttcta
     8041 ttttattttc tgataatagc ggctgtgcgg caaatgtgat tgcttcttgc cagttttttg
     8101 gttgatccga taattggatc atttcttctg ttaataattc agatagcata ggacgttgat
     8161 cctccttctt ttctaaaagt ttgttcattt tacgatttaa tacttgatag agcttttctt
     8221 cgtctacgcc ttcttttaat tcgatataag gcagtagcgc cgacaacaat tcttgggcag
     8281 aaggcattga aaatcctggt atcagccaat ctctttgtac ttgattgatc agctgatttt
     8341 tttccaagtc agtcatgatt ggatgcagaa catagagaga ctttttctta tcagtaagtg
     8401 tgagaggtac agtagaaaaa ataatatcgt aagaagtttc gtcaacctcg tgtaagcggt
     8461 cagctgaatt agtctctgta aaaaagatca tcgggaataa ttgctgaagt tcacttttta
     8521 agatcaatga agagctaatg ccacttggac agacgatcac ggctcttatt tttctatttc
     8581 tttcttctgc gtccgctttt ctgatctctc cgccaaatag tatagtgaaa aaaccaatct
     8641 cttcttcagg gatgctcttc cctattcgtc tttctaacgg aagtaatgct tttcttgtca
     8701 tttcgtagat ggaagcatat tgatgcttaa tttgatcgat catcacatta ggtaaataaa
     8761 aagaatagga aatacgaaaa taggcaggga ccaagtgata aaagacattc atcaataatt
     8821 cacgaaacga agcaaattct acggccatca ggaattccat gcgatggatg acttctgaag
     8881 aacattccag caaaaaatca agtgcaggct cctggacatc tccttcaacg atcgatgcta
     8941 agcaaagaga gaaaaatata atctccgaat tttgatgttc ggaatagcca aggccagtca
     9001 atacttgtcg actaatgtca gtgactgcaa gagaagccaa cagttcagca tcttttacca
     9061 gttccatgga tgtttttcca attcgaatgg ttgtcaaact caaataataa gctaattcat
     9121 ccattcgatt tggtgcgaat cgaagatctg atagtgaaat cgttttatct agcaaatctc
     9181 taactttggc gtaataatca atggaaaggg ttgcggttaa atacgtgagc ccaaatcttc
     9241 cagcaagtga ctgcatcaaa cgagcagtgc gattttttgc ttctcttctt aatattgctt
     9301 ctgaccctag cagcctaaaa ccagttttcc tagtatacga cagctgacaa tcttccactt
     9361 ctaattcatt tcttatcttt ttgatatctg acaggactgt ccctttagaa acctgaagaa
     9421 aatcttgaaa atgaaaaaca gaaacagacg ttgcagtcga ataaaccatc aaataaagaa
     9481 gatctcttcg ctcttgttcg gtatacacag cttcaaatcg agtaatatcc aaaaattttt
     9541 ctgcccagtc gattgctcgg aatttcttta tacggatacg ggtatcatcc acatattcgg
     9601 caaaaggagt taattgtttg atcgcctcgc ttgtatttac ttcgatgatc tctgctgctt
     9661 tttctagact gatttctctt ggtgttttca ggagataact aattttgtag ttcacagcgc
     9721 tgttcacctc ctacttatat tctagtatgg tagcgctttt aaatcgaggg gtgtttggta
     9781 caacttacag ttggattttt tttacgactg taccgaacga tttctatcga atggatacag
     9841 aaaaaaagca ggtgaccaaa tgtctcctgc ttttatgaat tgatttcgtc cgatcaatga
     9901 tgatcatgtt tattttttga ggtcagttct tcaaattttt ctttgacatc ttcaaccgct
     9961 ttttcgatat ctttttcaaa gtgattgggc tcattaggct ttgtttcctc ttctacagaa
    10021 tttgaaccag ataaagctgt ttctttattt tctgtttcac cagagtttgt gccagctaat
    10081 gattcttctt tgtcttctgt ttcaccagaa ttagttcctg ctaaagccac ttcttcttct
    10141 gttttagggg ccattgaacc tcttaatcgt ttgtgttttt caccgtcata atttgtcatg
    10201 ttggttcctc ctagtagttc tatacttttt catcgtacaa taagacagtt tatgcgtcaa
    10261 aaaacaaacc cgattcttac aaatatgtca tatttttcct tctgattctt gaatgctttt
    10321 gattccttct tccaaaattt tcatttgtaa gattgtttct tcatctttga tgactttttc
    10381 tgcgccatta actaatgtct cgtaaatccc ttcgtagaca cggctgtaat caccaacctc
    10441 ggaaattact ttttcttcat gataactccc ttgttcatct atataggtca atacaccata
    10501 atgttcgggc aaatcgatac caaagtcttc atgatcaggc atgtaaaaca ttttcagatg
    10561 ttcttcttgt cgatcttttg tttgtttcac gaacatccct ttttttccgt aaacgacgaa
    10621 acttggacgt tctttgatgc ggaaataact ggattttatc gaaattttca tatttccata
    10681 gaaaaaatcc agatcgaagt aatcattcat tcgtcctttc cctaaaagtt gacgtacatc
    10741 atagctgata tcatctggtt cgccgaaata agaaagaact tgatctactg tatgacaagc
    10801 atgtccatat aaatagctgt tcgcagtaga aaattcatgc gtattttctg gtacttccgg
    10861 tctgaaataa tcataatgca tttctacttc taaaatatcc cctaattttc cgctttcgat
    10921 tactttttgg actgtcaaga agtcagaatc gaaccgtctg ttttggtaac attggataaa
    10981 tagacctttt tcttttgcta atgcaaataa ctcttctgct tcctttgacg tttctgcaaa
    11041 aggtttctct actagtacat tcttaccatg ttcgagtgcc atttttccat attcataatg
    11101 aaattgacta ggtgtagtga tgacaactaa ttggatctca ggatcatccc aaagatcaga
    11161 cagctgatca gtatattcga tgtcttcgaa tgttttccac actggttcag atggggaaaa
    11221 aatttttttg atattgattt tgttcgtaag ttttcttgaa aaaggcaaat gatagcgatt
    11281 tgtactcttt ccattgccaa gataagcgat tgtaagcatg agtggtgcct ccttttattg
    11341 atgttttcag tatatcctag aaaagcgcag aaagtattta taatagctat tctggtaaaa
    11401 aagttcagaa aaacacttgt tatcattcgg tttggtgaaa ttaatgttca aagtaaggat
    11461 aaaaatcaaa aagatagtag cggaaccaag tgtttatcag taagatagaa tttggaggtc
    11521 agatcatgct tattgaagaa aaactaacaa aacaagagtt gtttaccacg acagagaaac
    11581 ggattgtcga ttacatccgt aggaatatcg aagctgcagt ctacatgacg attgaagaat
    11641 tagcaaaagc tacgtatact tcccattcag ctatcattcg tttatgcaaa aaaatgggat
    11701 tcagcggatt taaagaattc cgtttcgagc tggggcggga agttcatcaa ttgattgctc
    11761 agttcaatca aacgattgat cctaattttc ctttttcaag tgaagatagc actcagcaaa
    11821 ttgcaaagaa aatggcggat ttatcgatcc agtcaataaa aaaagcacaa ttgcagattg
    11881 aacatgaaga tttagatcaa atcggtaaag tattgaacaa ggcacagcga atctttcttt
    11941 ttgcaaaagg tgattctcaa atcacggcaa ggaaattcca gaataaaatg gtcaagttga
    12001 ataagttttt gatcatggct gaagaataca gtgattcttc ttggaatgca gccaatttga
    12061 ctagcgagga ttgcgccatt tttattagct acagcggtcg catccatcat tatgaacgaa
    12121 taatgaccta tttgaagcat gtagaggccc ctaccttgct gattaccgga aatcagcatt
    12181 cagaaatggc caaacaggct gatatgtgtc ttgttatatc gcaggaggaa tatgattttg
    12241 ctaaagtagc gactttctcc tctcaaattg ccttcgatta cgtattaaat acgttattct
    12301 cagtgatata ttctcaaaat tttgaagaga atatccgcaa cttgaaaaac aaacaagaat
    12361 tgatgcaaaa aggccttctt aaagaatatc ggaaaactta agaaaagatc ctcatttagt
    12421 aataagaaag atagtgtttg ataggctctc tttgatatca tcataaaaac aggctgaagt
    12481 tttctttttc aaacctcagc ctgtttgttt acggaacgat tcgaatagat atattataga
    12541 aatgaggaat agctaacttc tgtttcttat tttgaacgat tgacgaaaaa gatatataag
    12601 aaggaggaaa atcagtatac atgcagctaa caatccatac aggaaaaatc gtttgttctc
    12661 ggatactttc tgtacttctt tttgaacttc ttttgcatga taaggaaccc ggtgccctct
    12721 gacaagtaag cgatgactgt tgaccatata aggtgtacaa gttaggagtg tcacgaaatc
    12781 ctgccctttt tcgattctca gatcttttgt atcagtcggt tctaccgtct tgatctgatc
    12841 gacttgataa gcgagtgtcc gtccattgat ttcgatatag aaagaatcac ctaaagtcag
    12901 tttcggtaag tctgtgaaca actttgcttg aggcagtcct cgatggctgg aaataacggc
    12961 atgtgtattt tctccccccg taggataaga cgttccttcc aataatgagg cacctttttc
    13021 tagcagcaca gtattcgttt catcaaaaat tggcaaatgg acattgatct cagggatcgt
    13081 caaaataccg atcgtatgtt tctcaaaata ggttctatcc tttttcgcag gttctttttt
    13141 cttagtaaag ggatctgcac ctggatttcc accttctttt gctaattgct ggtttttctt
    13201 tgtcatgttt tcttggattt tagccatgac ttcttcattt tctttgaccg cttgttgctg
    13261 ataatgagag atgatctgct gatccagata attattcagc gcgtcgctaa caaatggata
    13321 agctagtatc cctattcctg agagaagcaa gaaaagcatc agcagatcta gaagtttcct
    13381 tttctttggt ttcatttgtg ttctccttgt gatgactgtt tgataataaa agaaaaagga
    13441 gcatttgcgt aatccgctgc tcctttttcc aattcttgca aaaagactaa atctgactgt
    13501 gcttgcgtct agcaaagtac agtccagcaa tcagtagaag gactactcct gcaccgatat
    13561 acacatagat tcccttaccg cctgttgaag gaagtgtacc tttgtgttta tttggtattt
    13621 tttcaggaga aatcaactgt cctgctgtta cataagattg ttcatcgata gtaaatgcta
    13681 cacggtttgt taatggaaca tatttttctg gcgctttcgt ttcttccaga tagtacgtgc
    13741 catattttag acctgtcaca tcgattaaac cgttacttgt ggttgtaaaa accgttgctg
    13801 attctttcgc cgatacccag ctgacggctt ttgtggatgg gtcgatcgat aaatatttcg
    13861 ctgtgtcact atcttgatca cgaacgacga attctgctcc agcaagtgtt tggtctgatg
    13921 taacgtcacc atctacttta acaaatcgtt tgcccccagt aacgacatcg actgacggtg
    13981 gtgtttgatc atttgtatgc ccgttatcga catttgcttg gttagaaaat cctttggttg
    14041 gatctgcttt ttcgttcaaa tgcatatagt aaacgaattt caatgtaccg ccaggagtta
    14101 atgaaggaat ataattcgga tcaactgaaa ccgtgaatcc gtctgtttgc tcagtgacag
    14161 aataatttac tgggtcgatt tctttatttc catcatataa ggcataagca tacgttcctg
    14221 aagaatcatt atcaaatgtt aaagcagcgt catgagtatc gataagtttg aatgttgtgt
    14281 acttgttttg cgttccttct ttatcagcga tacctaatgg gatattgaca gaaatctcat
    14341 attgaatttt ttcaccgatt gcgacatctt ttccattcaa ttgaggtgtt gttttatcaa
    14401 ctttagacgt atcatttttg atggtctttt ctactggtgt ttggctattt gccaggatct
    14461 caaaaggcgt gattgtttgc ttttcaatca tttccgcatt atcaggagct tttacttctt
    14521 ctaaattata ttttccaact tctaaatgat taacgatcaa ctggcctttt tcaatagatg
    14581 cctcggcaaa gtcattgttg ccgatgtcat aagaatgacc agtaatgaaa tgtttggctt
    14641 tggtttgatc agttgtccaa gtgtacaatc catctgtgac actttggatg tattttttga
    14701 cgcttggtgt tccttcttct ttagaaataa taaattctgc tccatttagt gcttcgtttt
    14761 cggcagtacc gatttttgta actttcaacg ttccatcatt accgactgta tttttagggt
    14821 agagatggat agtatctagt tcttctgtcc cgtatttata agagccatct gcttgtttga
    14881 tcatctcata tacagggaaa gctaaaacca tgtttgcggc agctgacact ccgtcttttg
    14941 gttcttcttt gatcgtgtag actgcatctt tcccattttg ttttttaggt aaagataaag
    15001 tgacatttcc atcagcatct gtcgttcctg aagcaactgg tgtaccagga gtcaaagact
    15061 ggactgcttg ttttgctgca tccacggacg ctcctttatc tcgttgcgca taaaattctt
    15121 gagtgacgtt ataaactgaa aatgaaatat cggctaatcc ttggtattga tcgaattcgc
    15181 tcatttcttt cccgctgttt tggattaaag gatcgggtaa atcagtcatt ttctttttat
    15241 gaaggatgac ttgagctgct gtctcctctg caaacacttt ttttgcgccg aagctgtttg
    15301 tgagtaatgg taaaataagg aaaaggactc ctaacataac gtttattttt ttatgatttt
    15361 tcattttttc actcctgttc tttttttgat aaacaaccag attgcaaagc ttgcgaatac
    15421 cataccaaca acgcctaaaa gtgtattttt cgtttcgttt gtttttggaa gaaatggacg
    15481 gctattttct tttttattga ctactttcaa tatactgtcc gcctctttat cctcagaaag
    15541 aaaagttgaa atggtaaacg gaatttctgt ttcacttaaa atataaccag aaggtgcttc
    15601 gatctctttt aatcgataac ttcctgtttt caacccggaa attgaaaaag cgccattttt
    15661 atcggaaatc aggtgaagcg catctgattc gttctttgtc cacttgtatc cgttggctgt
    15721 ttgctggagg taatttcctg ctttgttttt aataaggaat actgcgccag ctaatcgttt
    15781 atctggttta tttgtctcga ttttgacaaa ttgcttactc cctgtatata cagagacttt
    15841 ctttgtactg acaccaaaac caaaatccag tgttccgttg ttgttgattt ccttgttcgc
    15901 ctgtgctgta ctgcttagct gcatctgata agaaatggtc agtttttttc cagcatattt
    15961 ttccaacgaa gggatcgaaa ataaaacatc aaatccgtga ggtttcttgt gtatgacaaa
    16021 gccttctgtc agcttttctc cattcgatga aatcgttaaa ctattaggta aaaacgttaa
    16081 tgcttcatcc gcactatctg acaattcgaa cttttgatag tcaaggatat ttacaggaat
    16141 tgtcgtatgc aaggaataag tgattttttc accaatcgta aaatcatttc taggctcttc
    16201 taataccttc tcaaaaggtg gatcagggta gtcgttttct tcatttttag gatacaaatg
    16261 gatagttgaa agtttttgat tgttttgtcc atgtacagga agaacaacta ccatattctc
    16321 tgccttttct ttgacgactt ctggtacttt ggattcaatg aaaagatacg ctttatctct
    16381 tttttcttga tctttagcgg ccagagaaaa agaagcgata ccatcttcgt tgttaagagt
    16441 tgtagttgtt gcttctgcgg taaacatacc ggaagacgca ccgttttttg cgatctctgc
    16501 ttgtgcttct tctaccgttt tgcccttttc ccgtagatgg taaaaagaat ctgtgacatc
    16561 ataaacttgg aatgtgacac catttaatcc tcgatacgtt tgtaataaag ctttttcttg
    16621 tccgtcattt ggatgatttt tcggcagttg cccgtttggg aaaagcaatt tgtgtaaagt
    16681 aatctgaacc atttctgttt ctgtttctgt tgctacctga gtaaaagctg gtttaaatag
    16741 taacaagaag agacacatac taagccaacc aagttttttc atatttagct gccccctctt
    16801 tcttttttca aaaaaagata ccaaatagaa aacgcacaac caatcatccc tactagatag
    16861 attcctaaac ggccaattcc tcccgtttca ggtaatggta cttttgcctg attcgtgatg
    16921 tctaaagaaa tctggttgtt tttggctcct ggtgacagaa cagattcatg atcttgtcca
    16981 tccacttgaa ttgacccatc ttcgtgtata actatagtaa ctggctcttt tagaccttgg
    17041 tatccttctg gtgtaaaagt ttcggtcagc gtataagttc caggttttaa attttcgaat
    17101 agaaaggtat ctgtttcttg tccatctttt ggcgattcta agtccatttc tggtccctgc
    17161 agtctgaatt ttgctccttt taatgtctgt ccgttgtctt cttttttgtg taccgttaga
    17221 tcaaacgctt ttagtcgatt ttgatgagtg agcgtccata cattatttgt catctcggta
    17281 ttttttcccg tgtaaatgat ttttccatat ttatcgattt ggaattcata atttcccggc
    17341 gaagtatcat atccggcagg accagctgtt tcaacgactc gatatttacc aggtgtgagt
    17401 gaatcaaaac tggcaagacc tttttcattc gttgtttggc tgtcaattgg ctgataagta
    17461 ccatttgctt cttttttctg tagggaaaat gctgctcctt taagatttat ctctttgtcc
    17521 gtcccttgca tcgcatactt acgtactgct acaggaactt caacaaaagg attttcaata
    17581 gtcaactgga tcgtatcatc ggaagtagtg actgtttttc catcaatggt taccgtacca
    17641 tcagaagcga tcttgatttc ccaagtagtc tttttgctta atccatgccc ttctggagct
    17701 tttgtttctg tcagcgtata ggtcatttct ttttgcaatt tgacattttc tggaagagaa
    17761 taggttccgt cgccatgatc tgttagtaaa gtatcaatag aatctcctgt taatttgaaa
    17821 acagcgccaa taagatcctt ttcacctgta gaggaatttt tcgttatttt caagtttaac
    17881 ggtgtgaatt gtttagtatt tttccatgtc attgcatcta tttgttgaga atcgtatcct
    17941 ggtacaggta attctttgat tgtttggtaa ctgaatgctt gaccttgatt attgtattga
    18001 ggtagtgata atatctcttg ataactttct ccgctatttg cagataattt gtcaatgtct
    18061 gcacgttccc acgtatttgt tgtatcttta gctggtttaa tgattcgaat atatccgttt
    18121 ttccaagctg cagcatttgt cgtatgttcc cgttgaatct caaaagtaac ttgatctgga
    18181 cgatcagcta gattgttgtc aaattcttcc cataactttt tgatgtgaag actgactccg
    18241 ggagctttag cagatggtat accgaattca gctaattcat tggtatcaat gtttggctgg
    18301 aaagttgtcc tgccgttcat ttgataccag aaatttggat ggaagtcctc attttctgtt
    18361 tggattctca cttggtaatg gatttggatt tcttggtctt ttcctaaata gatctgattg
    18421 ctcttgatgg tatttccttc tatggaaata gatggagtga ccgttgtagg acttgtcccc
    18481 acactcttga ctgaaagtgt accaggctga taaatgaatg gttctgcgat tggatcattt
    18541 atttgtccat tgcttacagt tgctgagatc tgtacagctt ttttggctag gtattcagag
    18601 atatctgttg catgatcagc tgattcatag taaagatcgc ctttttcatc tgatgaaacc
    18661 atttgtcgca tacgagactc tacttctgct tttgagagac cagctgccgg atcgctttga
    18721 agttggatgc caagaccatg tatttcgatt cctcgttctt tgagtgccat cgcttctccg
    18781 atggttgcga taaacgtact gtcgattctt ctggatagat tgttttggtc aggtgcatca
    18841 tagattcttg agattagaga agtatttccc ggccgatctt gcgtattaga aaactgagtt
    18901 ccgtaataat tgctgcttga ttgtgcgtgt acccgctgta ctttatagga aaatgttggt
    18961 acaccatccg tcagcaaaac gatcactttt ttatgtccat ttggaacgga tagcatgctt
    19021 cctgcatctc ttagtgcttt ttgtgtaaaa gtaccaccat ttgtccgtga aggtgtaatg
    19081 gattttactt gatttttcac tgaatcaaat gaacccatct gtactgcacc gttactgtag
    19141 ctatatcctt cgcttgagta gccgacatat cccatattga ttttgtctgt gataccgcta
    19201 tctgctaaag tatcgacaaa acgatcgaca ccaatcttta cttcaccgat ccgattattg
    19261 tcgttcatac ttcctgacca gtctacgacc aatacgagat caagaggcgt gatatctttt
    19321 tgtacatttc cacgagcatt caaataaaca tcaaacaatc cgggtgtact tgtttccttt
    19381 gcatactttc gtatcgcata gtctgcttga ttaccggttc ctccatattc gatatacgaa
    19441 tgggtccgat cattaggatc tccattccaa gaatttatac catcccattg atttgttccg
    19501 tttttattgc cttgatgatc aagaacattt gtatttcctg taggttgcca actggcttta
    19561 ggataagtgc cctgatcatc tgttgtatat tctgtaggaa tcgtttgatt agatggcaca
    19621 ctcacagctg gcaaggataa tgtaggcaat gtaaatgggc tgacagaatt ttcgttttct
    19681 tttgatgatt cttcttctat ttcgtctggc tttggtactg acacatctgt atctttctta
    19741 ttttctgtct catcaataat gtcatattct ttattatcga cgatcagctt gtttgcctgg
    19801 ctgtggtaaa gtgctggatc gattggaaaa ctcagatcga tagtttgttt agtgtttgat
    19861 gagatttcca acgtaatttt cccgctattt acctgataag catagccatc tccttctttt
    19921 ctttccacat cacttaagta attttcgact agaccaattt ctttttctat cttttcagca
    19981 ctggtgttat tcgcatttag cttgatgttc agtgcttttt ttccagactg atccgaaatg
    20041 acttcagctg aaattgtttc taaaactgtt ggatcatctg tagtgtctgc tattgcagat
    20101 aatggcacaa agtttgataa actcaatatt atcagcatga aaataacttt cagtttcttc
    20161 cctgtggtcg tcatttttcc cctccttttt ctttttttct gttttgacgt gaatagtgaa
    20221 aattattaaa ataaacaggc gttacataat tttatttatt ttaatatgat aattattata
    20281 cgaagggcgc taaagggaat cacaccaatt ttcataccaa ttggtttcat ttatgaagtt
    20341 gattccttcg attaatgagc gtttcattga aagaaaaggg ttgagggaga aaaacatatg
    20401 atcaggttgt tggaattaaa ctatagaaag atggaaaaaa tcattcgaat tctgtccatt
    20461 tctgacaagt ggatgactaa aaaagaactt gccaaacgaa ttggtagttc agaatctaca
    20521 tttgtaaggt atatcgaaga gatcaaaatc cgctggggaa cggatgtaac gattcaatct
    20581 tcacaaaaat tggggtatcg tcttgaacat ttgaatgtac cagcttattt aaatgttctg
    20641 atggaaatgt cgcaaagttc tacaaacagt cgtttattaa atgagattat tgtcaatccg
    20701 ggtaaaacga ttgagtatta ttgtgaaaaa atttccatca gccggtcttc ttttacacgt
    20761 aagttgaaac aatgcaatca aattttagaa agctatttat taaaagtgat cgttgaccaa
    20821 ggttttcagt taactagcct taaaagtgag ctccagttgc gtatctttgt tacctttttc
    20881 tttttaagtt actacggaag acgcacgttg ccatttccaa tcgaaaaaaa gaagatcaaa
    20941 gaaattatgg tcaagaataa ttgtcagatg aatttgtttc aggaagaaag cccatatgag
    21001 caatctttct ttattatgta ttttgttgtg catctcatga gagaatccca aggaactcca
    21061 tttgaatgga aagctggaga ctcttatgta cagttgataa atatcgataa aaaagacttc
    21121 ttaatcttga aaaatctctt aggaaaattg aagtacagca gctatataaa agtagtgaac
    21181 tattttgtca atacgatttt ttcagaattt tcggagaaag aaaaaaatga aatcagtgag
    21241 cagatacgaa aaaatatgtc ctactgttcg ttttcatccg ctgattgcaa agaaaaaaac
    21301 gaagaatatg ttgttcaatt attaacgcaa atttattttt tctcccgtat tttcccatac
    21361 gatacgactc atttgacgat ccgtgcaact tactttgcac agcagatgca acaccgccag
    21421 atacgtctat atgctggatt tcgtgcagaa cttcaatttt attccactct actgaatcag
    21481 aatctgattt attatttatc tagctatatc ttttggatgc ttatcggtaa tcctgaaatt
    21541 caccattata cttctgataa aaaaatactg attatcagcg acttaagtct tcgacatagt
    21601 caatatattg aagaatatat cagtgatatt cttgctgtgc ataaaattca ctctgagaca
    21661 actgctatta ctgaagatca gctgtcgaaa tacaatcttc tcgagtatga tctgatcgtt
    21721 acgaatcaac ccattttgaa tgcccatgtt cctcatatat tgattgatga tagtgtgtca
    21781 ttcgcaaatg aagaagaact catccgctta ttcgagttat gaataatcaa aaaacagcta
    21841 tgtataaacc accctgataa aaatttcagg acaaatacac atagctgttt ctttttattg
    21901 tattccttca gcaatccaac gagatttata gcgaaagtct tttaacaact cttcttttgt
    21961 ccaattattt gcaatgagat ccgtcaaagt gttcattacg agggtcatgg aaaagtatag
    22021 gaaactaact gcttcttccg acgattgaat ggagaaatga ttgattctta ataattccag
    22081 ccatttttcg ttcattggag aatcagccga tagttctcta cgtttggttg tctgctgatc
    22141 atttcgctgg gttaacaagt ataacctttg ccaatagata ctttcgtgat ctaagtcgca
    22201 gcaccactct aaatattttt cgatccccaa aaagaaatgt tgtttgtttt cgctaatgaa
    22261 ttttaagata tcttgatgga ctatgtgaga actgcttttg atcgtataca aataagcatc
    22321 ttccaaatct tgaaaatatt tataaaaagc accgcgcgac atccccatct tgtcaacgat
    22381 ttctgagact ttgacttggc tgatgtgctg tgagcaaaat atctccagta ataggtcatc
    22441 gattctctgt ttctttgcgt tcgataaatt gaaaaaagtt tcttttggca tccacatctc
    22501 tcctttatct ctgattatac gtggatagcc cttcattgtc atgaaggatt cattccttct
    22561 ttttctcttc ttctgtcaag tacccatctt ccattcgata aacgcgatca ctccattgaa
    22621 tcatacggac atcatgagta accataatag tcgctttttg tttctcatga gcttctttaa
    22681 ctagtaattt gacgacttca tatgcatgat ctgtatccaa actagctgtt ggttcgtctg
    22741 ctaagatcaa gcttggctca ttatataaag cgcgagcaat tgccactctc tgtctttctc
    22801 caccagaaag gtctttagga taactatttt taagtgactg gatatctaaa gattgcagca
    22861 aggaatcgat ttttgttgaa tcttcttttt ttgaagtaac ttggtcaact agttcaaatt
    22921 gttcctttac cgttaaaaaa ggaatcaaat tagagctttg gagaataaat cctatttctt
    22981 taaagcgtaa atctgctcgt tttttttctg gaagctcagt tagtgagtga ccgttgatca
    23041 ctatttctcc ggaagtcggt gtttgcaatc ctcctgaaat ggtaaggaaa gtacttttac
    23101 ctgaaccaga aggaccaatg atgctgacga attctccttc ctttacataa aaatcaattg
    23161 ctttcaaagc tgagaccttt gtatgaccag aaccaaatgt tttggtaact tttttcatcg
    23221 ttaatatttt tttcattttt attaacctcc tattgctgta attgggtcta ctttggttat
    23281 cgtacgtatc gaaaacaatc cgcctagcat tgcgacaatg atcaaaatcc cactatatag
    23341 caaccattgt gaccaaaaaa cagcaaaagg catcgcgctt ggtaaaatca gactagtcaa
    23401 ataagcaaac aaaagagcaa gcagaacgcc taccacccca acaataaagg actgtgctac
    23461 taaagattta gcgatgaaga aatttttgat cccttgtgct ttcattactc caaaaatagc
    23521 agtcttctga agtgtcatca cgtacatgaa aataccgacc acggcagcaa ccactaagaa
    23581 caaaaagtat atcatcgcat ttaaggtcat attttgggca gaatagccag gaatactttc
    23641 aatgaatgtc ccaatagtta ataactgtag acccttcgat gctttttctt tcgaaatttt
    23701 agctgaatcc tttattacga tcccattgat cggctgttct tcttcagaag caaacggctg
    23761 ttgtccatac ttcaaagcag tccacgtatc tagggacgca tagatgacgg gagaaactgt
    23821 gtaataagtt tcaggaaaga taccgacaat cgtcaaagga tcgtcgtaac tgccaatttt
    23881 gatcgtatct ccaattttat agcctgaatc agccagattt tgagagatga tgatttcatt
    23941 ctttgtttta aattcatgcc ctttcgtgag ttttggtaat aaaaatgctt ctttcgttgt
    24001 gccgaaaact gtaacatttt ctttgttttt tcctttgatt gctccgctgt acaatccgat
    24061 tggtgctttc tctcctcctt caatgtattt cagatctcct cttgttaatt gtgaagctgc
    24121 aaatacttga tttgcatcct ccgacaaaac aatctcttgt gcgtcccaat catcgattgc
    24181 ttttttaaac tcttctgaaa gtccattcgc tagtcctgac aacatgaata cgacatacgc
    24241 aatcaagaac ataataccga cgatcaagcc atatcgtaat ttagaatagc gaatttcttt
    24301 gatagctaga aacatttgtt tttcctccct atgatagatt gatagtgaca aaatgtcact
    24361 tgttgaatag tgtaactcta aagtgacaat ttgtcacttg tgttgttttt ttcttatcaa
    24421 taaattgtct aagacatctt tcgataaaac cttaacatga gtcataaaaa atttatttaa
    24481 gccttattta tgctttccat gataaactaa agtgaccaaa tcaatgaggt gggaaaatga
    24541 ttaaactatt agtagtagaa gacgataaag tattatccaa taatataaaa gaaatactcg
    24601 ctgcaatcgg cgagataaca caagtttatg atggtagtga aggactgttc gagttggaaa
    24661 gtggagttta tgatcttgca atcttggatt tgatgatgcc ggaaatgaat ggatatgaag
    24721 tgttagcaaa aatacggaaa gaaaaaatac agactccagt attgattttg actgcaaaag
    24781 atagtttgga agataagatt gaaggatttg aacgaggtgc agatgattat ctaacaaaac
    24841 ctttctatcg agaagaactg atcatgcgcg tgaaagcatt attgaaacgc tcgctaggtc
    24901 tctttgatga aaatgttctg aagtataagg atatcagttg tgatttaaaa agcaatgaag
    24961 tcttgtataa agaacaagtt ttgcccatac aaggaaaaga gtttgaattg ctcgtttatt
    25021 ttttacaaaa taagggaatc attttgacaa aagagcagat atttgaccgg atctggggat
    25081 ttgattctga tacgacgatt acagttattg aagtttatat gagcaactta cgaaaacatc
    25141 tgcgaagtac tggattggat aaagaaatca aaaccttacg taatgtcggt tatatactgc
    25201 aaagggtgtg atgcaatgaa tggagatatt acacgaaagc aacgtagtcg cttttttgcg
    25261 acaaatgtag cagcatttgc ttttatcttt tttcttttgg gattcattac tttacagata
    25321 ttaaaccaat ctgcatacag agaaacagat ctgtcattag agaaaataaa ttttgattct
    25381 cgaacagtac aattagagat acaaagatat cagcaaggcg atcccttttt aaacccgact
    25441 caaacggaac aaaacagaac aaacatggaa cagccattaa tggaaccatc aaatgaccgt
    25501 tttaacactc aagtgatctt gtggtctaaa aatggtgaga tattgaataa agcagctatt
    25561 ggtgggcgtt tcaaccaaat caaagaatta aaaatggata cgaaaaatct gaataccatc
    25621 caaaatattg aattaaatga agacagtggc tcatctgccc tctctttccg ttcgattacg
    25681 aaaaaagcgg agacgaatca agatggcgtt gcctacatcc aaattttaga aaatacaaat
    25741 caaatcaaga attcattagc gacttttcgc acgattatga ttttgtgtat gattgttttt
    25801 tggattctct ctattggtat cagttattac ttgtctagtc taagcatgcg ccctattatt
    25861 ctttcttgga gaagacaaaa agaatttgtt gagaatgctt ctcatgaatt gcgcacaccg
    25921 ttaacgatta ttcaaaatag tttggaacat ctttttacca agcctaatca tactattatt
    25981 gaagaatccg aaagcatcgc tcaagcattg tcagagacga ggcgcctcac tggattgact
    26041 tcggatttgc tgacaatcgc tcggaatgat tctgatcagc agctgctttc aaagcaaatg
    26101 atcaatacac aagaatatat cgaacaactt gttaaaccat ttcaagagat ggccatcata
    26161 gatggaaaag agtttattct ccataacgat gcaacattag aagtaccaat tgatgaaaaa
    26221 aaaatccatc aagttttggt tattttgctg gataatgcat taaaatatac gaatccagaa
    26281 gataagatca ttttggattc atctgttaac cataaagaat ggctgattca cgtaaagaat
    26341 actggccctt ctatcagtga agaagatcaa gcaaggattt ttgatcgatt ttatcgggaa
    26401 gatcaatctc gggcgaaaga gactggcgga tacgggcttg gcttggcaat tgcaaaacag
    26461 gtaatcgaac agcatcatgg acagatttct gtatccaatt atcttccaca aggagtcgat
    26521 tttaaagtcc aattgccgtt gaagcctaaa aaataatgct tatccctaaa aaagggctgt
    26581 gacaaaaatc ttgtcacagc ccttttttgt tgcgaataaa cggtgttcca gaagtagctt
    26641 cttcggaaat aagccgaaat ttctttaagc tgaccacttc tgtcacaacc tctttagctt
    26701 acgcttacga ttttttattg cggtcttcaa tgtgtacatc accgcttgtg tcaatatcca
    26761 attcttctcg gctaacatct tctgtcactt tttcaacatc ttcatgttct tctttatgaa
    26821 tcccaacttc ttcacgaata actgtatgtt tgcttacatc tacttgctct tctttgattg
    26881 ggatagtaat cgtgtcatct tccaattttt cgtctgttcc ctgtgaagat tgatctgtta
    26941 ctggtttgcg ttcgatgacg atttcttcac gtttgaccgg tacttctacc gtttcagtat
    27001 cgttgaccac atgtttgtgg atgtcgactt ctccagttgt cacatcatgt gtatttacat
    27061 ctaatttttc ttcttttagt tgaattgtat catcatctag atttggtttg cgatcgatgt
    27121 cttcagtctt gcttgtaccc attgtatcag tctcgccagg tacgtccatc ggatcagttc
    27181 ctggtgtcat attcaatgga tccgttgtat tttgaaagcc tgtcgtgcca cctacgtcca
    27241 ttggatctgt tccagtgcca gtttctaatc cagtccccat tgtgcttgct gggctaacag
    27301 tatcttgtgt atccattgta tcttctgttg tttctggctg acgatagcct tttactgtga
    27361 tgacaaattt tccatcagaa atatctcctc tatattgata taaaggatca ttttctggtg
    27421 tagccgtctc agaatcatag gtatcaaaag aaaaagtatc ttttattttt tcccataaag
    27481 aacggtcttc ttcaccatcc atccgatttt catctgtttc aacatctata ccagacaatc
    27541 cgtcaaatcc tagtctagat gctgcttcat cagtagtata aataacgata tcatctcgtt
    27601 catacccttc gttacgcaac cgttcaatta ctgataatgc ttcttccctc gtactatagc
    27661 ttccgatgac cgttaaattt ttctcttcca tgtaaattcc tcctagcaaa tagatttatg
    27721 attggtatat tgttacttat aaatttattc tataggagac gattgttttc tacaaatatt
    27781 tagattggaa tttgattaaa acaatttaat tgaaatttga aaacat
//
LOCUS       DAZKSR010000032        27592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000032 DAZKSR010000000
VERSION     DAZKSR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.032"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG8XG_001209"
     CDS             complement(287..649)
                     /locus_tag="ACG8XG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773136.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG8XG_001210"
     CDS             complement(723..1103)
                     /locus_tag="ACG8XG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0773137.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG8XG_001211"
     CDS             1185..2360
                     /locus_tag="ACG8XG_001211"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0773138.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG8XG_001212"
     CDS             complement(2437..3021)
                     /locus_tag="ACG8XG_001212"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0773139.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG8XG_001213"
     CDS             3222..4220
                     /locus_tag="ACG8XG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0773140.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG8XG_001214"
     CDS             4332..4841
                     /locus_tag="ACG8XG_001214"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0773141.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACG8XG_001215"
     CDS             complement(4871..5530)
                     /locus_tag="ACG8XG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0773142.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACG8XG_001216"
     CDS             complement(5532..6296)
                     /locus_tag="ACG8XG_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0773143.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACG8XG_001217"
     CDS             complement(6304..6981)
                     /locus_tag="ACG8XG_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0773144.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACG8XG_001218"
     CDS             complement(7018..8403)
                     /locus_tag="ACG8XG_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0773145.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACG8XG_001219"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8XG_001219"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8XG_001220"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8XG_001220"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0773146.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACG8XG_001221"
     CDS             9342..10199
                     /locus_tag="ACG8XG_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773147.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACG8XG_001222"
     CDS             complement(10280..10615)
                     /locus_tag="ACG8XG_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773148.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACG8XG_001223"
     CDS             complement(10682..12052)
                     /locus_tag="ACG8XG_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0773149.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ACG8XG_001224"
     CDS             complement(12130..12666)
                     /locus_tag="ACG8XG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0773150.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ACG8XG_001225"
     CDS             12975..14456
                     /locus_tag="ACG8XG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773151.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="ACG8XG_001226"
     CDS             complement(14559..15266)
                     /locus_tag="ACG8XG_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0773152.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="ACG8XG_001227"
     CDS             complement(15345..15683)
                     /locus_tag="ACG8XG_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0773153.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="ACG8XG_001228"
     CDS             complement(15794..16756)
                     /locus_tag="ACG8XG_001228"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0773154.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="ACG8XG_001229"
     CDS             complement(16771..18102)
                     /locus_tag="ACG8XG_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0773155.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="ACG8XG_001230"
     CDS             complement(18110..18790)
                     /locus_tag="ACG8XG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0773156.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="ACG8XG_001231"
     CDS             complement(19009..19488)
                     /locus_tag="ACG8XG_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0773157.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="ACG8XG_001232"
     CDS             complement(19615..21294)
                     /locus_tag="ACG8XG_001232"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0773158.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="ACG8XG_001233"
     CDS             21842..23344
                     /locus_tag="ACG8XG_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773159.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="ACG8XG_001234"
     CDS             23357..24019
                     /locus_tag="ACG8XG_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773160.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="ACG8XG_001235"
     CDS             complement(24120..24323)
                     /locus_tag="ACG8XG_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0773161.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="ACG8XG_001236"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="ACG8XG_001236"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0773162.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="ACG8XG_001237"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="ACG8XG_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0773163.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8214 a         5760 c         4671 g         8947 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taataaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tg
//
LOCUS       DAZKSR010000033        26844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000033 DAZKSR010000000
VERSION     DAZKSR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.033"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..354)
                     /gene="rbfA"
                     /locus_tag="ACG8XG_001238"
     CDS             complement(7..354)
                     /gene="rbfA"
                     /locus_tag="ACG8XG_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0773164.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(381..2687)
                     /gene="infB"
                     /locus_tag="ACG8XG_001239"
     CDS             complement(381..2687)
                     /gene="infB"
                     /locus_tag="ACG8XG_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0773165.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2700..3011)
                     /locus_tag="ACG8XG_001240"
     CDS             complement(2700..3011)
                     /locus_tag="ACG8XG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0773166.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3008..3301)
                     /locus_tag="ACG8XG_001241"
     CDS             complement(3008..3301)
                     /locus_tag="ACG8XG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0773167.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3323..4498)
                     /gene="nusA"
                     /locus_tag="ACG8XG_001242"
     CDS             complement(3323..4498)
                     /gene="nusA"
                     /locus_tag="ACG8XG_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0773168.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4521..4994)
                     /gene="rimP"
                     /locus_tag="ACG8XG_001243"
     CDS             complement(4521..4994)
                     /gene="rimP"
                     /locus_tag="ACG8XG_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0773169.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5139..9497)
                     /locus_tag="ACG8XG_001244"
     CDS             complement(5139..9497)
                     /locus_tag="ACG8XG_001244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0773170.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(9709..11418)
                     /locus_tag="ACG8XG_001245"
     CDS             complement(9709..11418)
                     /locus_tag="ACG8XG_001245"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0773171.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(11486..12754)
                     /gene="rseP"
                     /locus_tag="ACG8XG_001246"
     CDS             complement(11486..12754)
                     /gene="rseP"
                     /locus_tag="ACG8XG_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0773172.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12915..13715)
                     /locus_tag="ACG8XG_001247"
     CDS             complement(12915..13715)
                     /locus_tag="ACG8XG_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0773173.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13712..14524)
                     /locus_tag="ACG8XG_001248"
     CDS             complement(13712..14524)
                     /locus_tag="ACG8XG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0773174.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14719..15276)
                     /gene="frr"
                     /locus_tag="ACG8XG_001249"
     CDS             complement(14719..15276)
                     /gene="frr"
                     /locus_tag="ACG8XG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0773175.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15279..16001)
                     /gene="pyrH"
                     /locus_tag="ACG8XG_001250"
     CDS             complement(15279..16001)
                     /gene="pyrH"
                     /locus_tag="ACG8XG_001250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0773176.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16137..17018)
                     /gene="tsf"
                     /locus_tag="ACG8XG_001251"
     CDS             complement(16137..17018)
                     /gene="tsf"
                     /locus_tag="ACG8XG_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0773177.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17117..17899)
                     /gene="rpsB"
                     /locus_tag="ACG8XG_001252"
     CDS             complement(17117..17899)
                     /gene="rpsB"
                     /locus_tag="ACG8XG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0773178.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18258..18737
                     /locus_tag="ACG8XG_001253"
     CDS             18258..18737
                     /locus_tag="ACG8XG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0773179.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18954..20045
                     /gene="phnW"
                     /locus_tag="ACG8XG_001254"
     CDS             18954..20045
                     /gene="phnW"
                     /locus_tag="ACG8XG_001254"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0773180.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20038..20166
                     /locus_tag="ACG8XG_001255"
     CDS             20038..20166
                     /locus_tag="ACG8XG_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0773181.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            20170..20715
                     /locus_tag="ACG8XG_001256"
     CDS             20170..20715
                     /locus_tag="ACG8XG_001256"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0773182.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(21169..22860)
                     /gene="argS"
                     /locus_tag="ACG8XG_001257"
     CDS             complement(21169..22860)
                     /gene="argS"
                     /locus_tag="ACG8XG_001257"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0773183.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23280..24227)
                     /gene="arcC"
                     /locus_tag="ACG8XG_001258"
     CDS             complement(23280..24227)
                     /gene="arcC"
                     /locus_tag="ACG8XG_001258"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0773184.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24342..25361)
                     /gene="argF"
                     /locus_tag="ACG8XG_001259"
     CDS             complement(24342..25361)
                     /gene="argF"
                     /locus_tag="ACG8XG_001259"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0773185.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25452..26681)
                     /gene="arcA"
                     /locus_tag="ACG8XG_001260"
     CDS             complement(25452..26681)
                     /gene="arcA"
                     /locus_tag="ACG8XG_001260"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0773186.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7144 a         5778 c         4784 g         9138 t
ORIGIN      
        1 ttttgattag tctttgttca agtcgcgaag caattcatcg atatggttgc catagacaac
       61 tgattcatca agttcaaaga acaattcagg tgtcttataa atacttagat tgtgccctaa
      121 ctctcgacga atcaatcctg atgctttatt caatccatct tgtgctgttt tcttatctga
      181 tgctaagtca gataaaatgc tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt
      241 cacatctgtg atcgttacgt tctccacacg agggtcacgg acctttttgc gcaaaatatc
      301 attgacttct tttaagattt cttgaccgac tcggcggtca cggtagttag ccataatgat
      361 tggcctcctt ttatcagttt ttagtcattt ttgacttctt ccatgataaa tccttcaatt
      421 acgtcatcta ctttgatgtc attgaatttt tcaatcattg caccacattc ataacccatt
      481 ttgacttctt tcacgtcatc tttgaatcgt ttcagacttg caagttggcc ttcatagatt
      541 acgattccgt cacggattac acggacaccg ctatctctac gaatatatcc ttctgttaca
      601 taggcacctg caatcgtacc aactttagaa actttgaagg tttcacgaac gatcatttga
      661 ccggtgattt tttcttcaaa ttcagggtct agcatccctt tcatcgcagt ttcgatttct
      721 tcgatggctt tatagatgat acggtgaaga cgaatatcga cttcttcagt atcagcttgc
      781 acttttgcct gcggtgtagg acgaacgttg aaaccgatga taatagcgtt gctggctgct
      841 gctaaggtta catcactttc attgattgct ccgacagctg aatggacgat ttttacacgg
      901 acaccttcca catcaatctt cttcaatgat gcagccagtg cttctgctga accttgtaca
      961 tctgctttga tgattacgtt aacttctttc aattcgcctt ctttcaggct ttcaaataag
     1021 ttatccaaag tcacacggct tgtggcagca cggttttcta ataatgcacg tttcgcgcgt
     1081 tcttcacccg ctgcacgtgc tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct
     1141 tgaggtacat cattcaagcc agtgatttct actggcgttg caggtcctgc ttctttatcg
     1201 cgacggccaa gatcgtttgt cattacacgg acacgtccgt aagtattacc gacaacgatt
     1261 gggtcaccaa catgaagtgt tccttcttgg acaagtaagg ttgacacagg acctttacct
     1321 ttatcaagac gtgcttcgat cactgttccg attgcgcgtt gtgttggatc tgcttttaaa
     1381 tcttctactt ctgcgactaa caggatcatt tccagcaatt cttcgatgtt ttggccgaat
     1441 ttcgctgaga tctcaacaaa gatcgtttct ccaccccatg cttcaggaat taactcatac
     1501 tcacttaatt cttgcataac atgttgaggg tttgcacctg gtttatcaat tttatttaca
     1561 gcgacgatga ttggaacgcc tgcagctttg gcatggttga ttgcttcaac tgtttgtggc
     1621 atgacgccat catctgcagc aacaactaag atcgtgatat ctgtgatact tgctccacgt
     1681 gcacgcatac ttgtaaaggc cgcatgtcct ggtgtatcca agaaagtgat tggtttacca
     1741 tcaatatcga tctggtaggc accgatatgc tgtgtgattc cgccggcttc gccgcttgtt
     1801 acacgtgaat ggcgtaatgt atccaataaa gttgttttcc catggtctac gtgtcccata
     1861 atcgttacga ctggcggacg tgagactaat ttatctggat cgacttcttc tgcctcgaag
     1921 aatttatcga tatcagcaat gtcaacttgg actttttctt gtggttccat gccatagtcc
     1981 gtcgctaaca gttcaatcgt atctttatcc agtgcttggt tttgattgac catgacacca
     2041 agcataaata attttttaat gatttctgct ggctcacgat gaattttttt cgcaatatct
     2101 gctacgttca taccttcagt atactctaat acatctggca attcacggaa tttgcgtggt
     2161 ggtacagctg gtttagttga ttgttgctgt ttgccttttt tacctttttt attataacga
     2221 ttacggttat tattattaaa tttgccgcga ttgttattgt ttggtttgtt gttattgtta
     2281 gtacggtttt gattgccgcc ttggctcgtt ccttgacgat tttggctgct ggtatttccc
     2341 gtattatttt ggtttgttgt tggttttttt ccttgattca cttgaccgct cccttgaccg
     2401 cgatcttgat aactgcggtt atttttattt ttttgttgat tgttcgttgt ttctttttta
     2461 cgttgatcgt tcggctgttg attggctggt tttctgcttg cttgttgtgc agcttgttgt
     2521 ttcgtgcttg ggtttttgaa cgcttgttgc aatttcttct catcattgct agtaatcgca
     2581 cccatatggt ttttcacatc gatgccaagt tgttgtgctt tttccacgac gtctttacta
     2641 gattttttga gttcttttgc taattcatag attcttttgt tgcccatgca atcaccttcc
     2701 taaccttcaa ttagctcctt ggtcttcttc gcaaatccgg catccattat tccgaatacc
     2761 ttgcgaggtt ttccgatcat ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga
     2821 acttcataat atgaactctt atcttttatc ttttttcttg tattctcgct tgcatcggtt
     2881 gccacaaaca ccaattttgc tttttgtttg cggatttcag caatggtcag ttcttcaccc
     2941 gtgattagtt ttcccgcgcg catcgctaga ccgatgagat tcatggcttt ttgccgatta
     3001 actgtattca ttttccaaat aactctttac gtgctttttg gtgagtgaca tagtcaagaa
     3061 gttcttgata aaactcatct gttaatgttg tttcgagtac tcgatcgaga atttttttat
     3121 cccaagcttg ttgtacttct tccggctcaa gtgagacata cgctcctcgt ccaggcattt
     3181 ttccagttgg atcgatcgat acttcccctt cttttgaacg ggtgattcgg accatctctt
     3241 ttttaggttt catttcacct gaaaccacgg atttgcgtaa aggaattttc ctttgtttca
     3301 tctctttgcc tccttactaa agttaagctt ctggtttttc ttcgaccgtt tcattgttga
     3361 atgcaattgt ttcatattca tcgtctgtta aatctgactg aacgattgct tcatcatgca
     3421 gttcttctgt ttctttttgt tgttcttgat tttcataaaa ttccgtcatg tcagattctg
     3481 atttgatatc gattttatgg ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt
     3541 taccgattgc tagcgataat tggtagtctg gaacgacaac tgtgcaggcc tttggatttg
     3601 tctcatcgaa gatcacatcc atgacttgtg caggattcaa tgcatttgca atgaatacag
     3661 ctggatcttc attccattcg acgatatcca tgttttcgcc ttttaattca ttgacgattg
     3721 cttgaactcg ttgtcccttt ggcccgacac atgtaccgac tgcatcgata tttggatcat
     3781 ttgagcgaac tgccactttt gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa
     3841 cgattccatc atagacttct ggtacttctt gctcaaataa gcggcgcaat aggtctggat
     3901 ggctgcggct aacaaatact tgcggacctt tagacgtatt ttctactcgg gaaacataaa
     3961 ctttgattcg atcatgtggt tgataaaatt catttggcat ttgatcttgt ttagaaagaa
     4021 ctgcctcaat tttaccaaga ttgacataaa tataacgttt atcctggcgt tcgacgattc
     4081 cttgcatgat gtctttttca taagcactga attcgttata aataatcgtt ctttctgctt
     4141 cacgtacacg ctgtaaaata acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt
     4201 ttggtgttac ttcaaaacgg atcgtatcac cgatctcata tgctggattg attaaaagtg
     4261 catctttcaa tgagacttcc aactgtgaat ccatcacttc ttccgttact tcttttaccg
     4321 catagacatg gatctttcct tttttctgat caaattccac ttcgacgttt tgtgcttggc
     4381 catagtgacg tttgtatgca gaaaccaatg ctgcttctaa agcatcgatc acgatttctt
     4441 ttgagatccc tttttcagct tctaaagcgt ctaacgcatt taacatttct ttactcattt
     4501 tttctgattc ctctccgtga ttaaaattgg atagctaagc gagcttttgc aatatttttt
     4561 cggtcaaaag tcaattcttt ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca
     4621 gcatcaaatg attgaagaaa tccttcatat tgtttttcgc catctactgg ttggtataag
     4681 gagacatgga cataatcacc aagtgccttt tcatagtcgc tttctttttt caatggccgc
     4741 tctgctcccg gggaggatac ctccaggaaa taagcttgag gaatcggatc tggatctgct
     4801 gcatcaagtt tttcactcag tttctcactg acaaaggcgc attcttcgat atcgataccg
     4861 ccttctttgt caataaatac tcgtaagaac cagctttttc cttcttttac aaattcgact
     4921 tcgacaagtt caaaattttg ttcatctaaa atcggtgtca ccatttctgt gactgtttca
     4981 acgacgctac tcaaaagttt cgcctcctta aaaaattaag tgcgtaattc cattaaaaag
     5041 agtgagcgaa aaatccgctc actcacagtt agtatcatta cctataagaa ctataccata
     5101 ctctgcttaa aattacaagc agacaggaaa acagatgctt atagcatatc gaataacgat
     5161 aattgatttt catctggtag atctttcaat acgccattgt cgttcatata ttcgatcaat
     5221 gtttttgaaa ctttaccgcg ggtggcaaga tcttctttgg ataaaaatgg accgtctttt
     5281 cttgcttcaa cgatttgttt tgccacgttg gttcccaaac ttgggacagc tctaaatggt
     5341 gcgatcaacg tatcaccttc aataacaaag ttaacggcat ccgatttata caaatcaatc
     5401 attccaaatt tgaatcctcg ttccagcatt tcattggcta actccagcac tgtcagttga
     5461 ttcttttctt ttacggaagc atctaatcct ttgtccgtga tttctttcat tgcttgtttc
     5521 acagcatctt ttcctttgca catggagact agatcaaagt cgtctgcacg tacagaaaag
     5581 tatgcacagt aatacaaaat agggaaataa accttgaaat aagcgacacg aagagccatc
     5641 aatacgtaag ctgcagcatg ggctttcgga aacatatatt tgatttttga acaagaatcg
     5701 atataccagt cgggcacatt attttctttc atcgccgata gataggtttc tcgcaattca
     5761 tcagggatct tgttccattg tcctttacga acagtttcca tgattttaaa tgccatcccg
     5821 ctatctaatc cagcatggat caaataaacc ataatatcat cacgacagcc gattacttcc
     5881 gccagtgtcg catctcctcg tcgaatcaat tcttccgcat ttcctaacca tacgtctgta
     5941 ccatgagata gtccggaaat ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct
     6001 agcatccctc gaacaaaacg agtgccgaat tctggaatac ctaatgtccc tgttttggaa
     6061 taaatttgtt cctgacttac tccaagtact tcaggaccgg caaatatccg catcacttcc
     6121 gggtcatccg tagggatcgt ttgtggatcg atccctgata aatcttgaag catccgaata
     6181 acagttgggt catcatgccc tagaatatct aatttcaata cattatcgtg aatcgagtga
     6241 aaatcaaaat gagtcgtttt ccattccgaa ttttgatcgt ctgctggata ttggatcggc
     6301 gtaaaatcat agacatccat ataatcggga ataacgatga tcccccctgg atgctggccg
     6361 gttgtccgtt tgactccagt agcgcctttt gctaatctgt cgacttctgc acttcgaaac
     6421 tgcagattat tgtccctttc gtatcctttt acatagccat atgctgtttt gtcggcgact
     6481 gtaccaatcg ttccggcacg gtaaacatag tcttcgccaa acaatacttt cgtatagtta
     6541 tgggcttctg cttgataatc acctgagaag ttcaaatcaa tatcgggcac tttgtctcca
     6601 tggaatccta aaaatgtctc aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca
     6661 cattttggac attgtttttc tggcatgtca aatccagaac catacgtacc atcttcaaaa
     6721 aactcggaat attggcactc tggacaataa taatgagggg ctaacggatt aacttcagtg
     6781 attcctgtca tcgtcgcaac aaagctagag ccaacagagc cacgtgaacc tactagatag
     6841 ccatcttcat tacttttatg cactagtttt tgggcgatca gataaataac cgagaagcca
     6901 ttaccattaa tagaattcaa ctcttttttt aatcgttttt gaatgatctc aggtaacgga
     6961 tctccgtaca tttgttttgc ttttgtgtaa ctcaattctg atatttcatc ttctgaacct
     7021 ggaatcttcg gtgtataaag ttcatctttg actggaatca cttcttcgca gatatctgcg
     7081 attttattcg tattttctac tacgatctct ttcgctgttt cttcccctaa aaagtgaaat
     7141 gcagttagca tctcatcggt tgttcggaaa tggacatccg gcaaactatg acgattcaac
     7201 ggattcgctc cgcccataga attgatcaaa atttttcggt aaatggcatc ttcttcgttc
     7261 agataatgta cattacctgt tgcaacaacg attttatcca aactttttcc gatttctacc
     7321 aaattttgga taatttcttc caaatcatgt tcatttttta ccaattcttg ctcgatcaaa
     7381 ggcgcataga ccgcttttgg cataacttca atataatcgt agaattttgc tcggtttctt
     7441 gcctcttcta cccctttttg catcatggct tcgaatattt ctcccttatc acaagctgag
     7501 ccgatcaata gattttcacg catctttttc aattgggagc gtggtattcg tgggacgcgt
     7561 tcgaagtatt cgacattcga cattgaaatc agtttgaaca aatctttcag tcctgcctga
     7621 ttttttgcta aaatcgttac atggaaaggt cgagcccttt tgtaagaatc tccttctccg
     7681 atatgctcat ttagctgatc atgataaagc atgttatgat tgtccattgc ctctttcaca
     7741 aagatccatg ctaagtgacc agttgcttca gcatcataga ttgctcggtg atgctgttcc
     7801 aagctgacac caaatttttt agataacaca cctaatccga aacgtttgaa ttgaggatac
     7861 agatatctag ctaattccaa cgtatcaatg actggattgg ccgcttcagg aataccatac
     7921 cttgcgtagc tagtattcaa gaaccccata tcaaaagccg cgttgtgggc taccaaaatt
     7981 gtatcttttg aaaattcaag gaacatccgc aatacttctt cttccgactt tgatcctcga
     8041 accattccat ccgtgatacc agtcagatct accgttgttc gtgacaaagg atgtcctgga
     8101 tcgataaatt catcaaagct ttcgatcaca tttcctttat acattttgac tgcggctagc
     8161 tcgatgatcg tgtcatatac tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat
     8221 gtggcttcac tcaatgcttc atgtgcatca ttataagcaa ttgggacacc atcatctacg
     8281 acattcgctt ccacgccgta taagattttc acacctgctt tttttccagc actatgtgct
     8341 tccggaaagg cttgcgcacc accgtggtct gtaatggcga tagctcggtg tccccatttc
     8401 cctgcttgtg ccacaagatc agagatgctg tttgttgcat ccatcgtact catattgcta
     8461 tgaacatgaa gctctactcg tttttcccct tcaggagcat aatctttgcg aggtgtatgt
     8521 ttgacttcta tgatatcttg cgcattcatg accaaatcac ggacaaacgt atcttcttga
     8581 atacttcctc gtacctttaa ccagctaccg acactgatcg catcaaatac ttgttcatct
     8641 ttttcgccgt tcgagaattt tttgacgata aatgatgaag tataatctgt gattttcaaa
     8701 gtcaagatct tgcgttttga acgtagttct ctgacttctt tgtcgaatac gtacccctca
     8761 agtgtgactc ttcgttcttc ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct
     8821 ggaatatttc gtcctagctg gatgtggatg ggtccttcaa gagctggcga ttgctctttt
     8881 ctttcctttt tcttttgttc gtggacaact agactttcag ctgcttgctt cataaaggct
     8941 tctgcctgtt cttgtttgcg ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca
     9001 tccacttctg gttcgatacg aaattttggg aatccgtaac tgacgaacaa ctcttctact
     9061 aatggcaagt attgttgctt caaataagaa atagctcctg tactgtcgat tggcaagatg
     9121 actttacgat cttttatcac aggtacctgc gttttcaata cttgctgtgc tagtggtgta
     9181 tcgcattgct gattttccaa agcttgggcc cagtaatctt gcaagagctg ttcatcgaat
     9241 gtctggtcat ctgctaagat attcagtttg acttgtgcaa tatctttaaa agcgatagtc
     9301 agatgctgag tcaatgaccg atatagcata ataggcaaaa ttttggaaaa atgcagcgta
     9361 aattcccata aacgcgattt acgatggacc actacactat ccatttcacc agcagtaagc
     9421 agtggatgat ttttttctgc ttcctctagt tggatttgtt ccaacagttt ttcaaataaa
     9481 tctcgttttt ctgacaaaga tatacgccct ttctgatgag aaacaatgca attgatcgca
     9541 tcttatcctc ttctttttca gtacctttta gtatactcgt tttttctgag ttttacatta
     9601 aaaaattgga actgcgattc aaatcgcagt tccaatttta aaagcgaatc atcagattgt
     9661 caaaaacagc tgattcagct atttttattt tctgtgtttt aacgcttatt attcgccttg
     9721 gttcaagagg attggtaaag tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc
     9781 agttttcttg attttcactt ctacgattcc atccactgct tttttaccga cagtgattct
     9841 gattggacag ccaatcaaat cagaatccgc gaatttcact cctgcgcgtt catttctgtc
     9901 atcaaccagt acttcatagc cagcttttgt catcgcttct tctagttcat tcgtcaggtt
     9961 tgtttggtat tcgtctttta gattcatttg gacgatatgc aaatcaaatg gcgcaattcc
    10021 ttttggccag ttgattcctt gttcatctga atgttgttct acgattgctg ataataaacg
    10081 acttacaccg ataccataac agcccataat cacaaatttt tctcgtccat tttcgtctag
    10141 tacagttgct cccatagatt cactgtaacg agtgccgatt ttgaagatat gtccgatttc
    10201 gattcctcga gtaaaggcaa gaacaccatt gttgtctggt gaaggatctc cttcttgtac
    10261 aaaacgaaga tcttcataag aaagtacagt gaaatcgcgt tctggattca catttttata
    10321 gtggaatcct gtttcgttcg ctccagtaat ggcattcgct aaatcttgga cataacggtc
    10381 tgcatatact tttacgtcat ctgaaacacc gactggtccg acagaaccaa actcagcacc
    10441 tagatatttt tgtgcttctt cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt
    10501 tttcagtttt acgtcattga cttcatggtc tccgcgtacc aaaacaagga caggttgttc
    10561 atccgcaatg aacaatacag acttgatgat tttttgaggt tcaacagaaa agaaatctgc
    10621 tacttcgtca attgatttta cgttaggtgt agctactttc tccaattcaa gctgtgtttc
    10681 atgcgatttt tttgatacat agtagcttgt agccatctcc aagtttgcag cataatcact
    10741 ttcagtcgaa taacagatcg tatcctcccc gatttctgag atcgccatga attcttttga
    10801 gtctttcccg cccattgcgc caccgtctcc gataatcgag cgaaattcta agccacaacg
    10861 cttgaatact tcggtgtagg ctttttcgta atctttatat gtctcgtcca aactttcttc
    10921 cgttgcatgg aaagaatacg catctttcat gataaactct cttccgcgaa gcaaaccgaa
    10981 gcgaggtcgt ttttcgtcac gatatttagg ttggatttga taaagattta aaggtaattt
    11041 cttatatgaa ttgatttcat tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc
    11101 aaggatcatt tttcgatcat tgcggtcttt gaaacgatac aagtttggtc catatgtttc
    11161 gtaacgcccg gattcttccc aaagttctgc tggcaagatg gcaggcatca gcatttctac
    11221 cgctccgatt ttttcaaatt cttcccgcat aatgcgtttc agtttttcca atacacgatt
    11281 tgctaatggc agatatgaat aaatacccgc tgatacttgg cggatatatc cagctcttag
    11341 caagatttga tggcttaata cttctgcatc atttggaact tcgcgtaatg ttggaatcaa
    11401 catttttgat tgtctcattc tatatactcc tttatttcct caaaatagtt atcgtttgtt
    11461 attataccct atttgtcaac tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa
    11521 ctagcaccat caacaacatt aaaaatccaa agccagctaa tgtcaatatt ccttcttttt
    11581 cttgacttaa tggtttcccg cgaatccctt caaaaatatt caagactaat ttcccgccat
    11641 ctaaggcagg aatcggaagc agattgacga ttccgagatt cattgaaaga agcgccatca
    11701 aactaataac agttgttaca ccttgatttg ctgcttctga agacaattgg tacatcatga
    11761 caggtccgcc taatttatct aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa
    11821 tttccaatga accgctgaaa gcttgacgcg tccctccaat gatcttatcc ataaaacctg
    11881 tattcattgg ggcttttata ccaagttggc caactttttc gccatttgac tctacagatt
    11941 tcggtgtcac gtcgacagat tgcacctgac cttctcgttc gatcttaaag tctaatgttt
    12001 tatccggatt tttcgtaatg actgttgtca gatcattcca tgaatggatt tcttttccgt
    12061 ccacactgac tacctcatca ttttctttta agcctgcttc tgccgcagct ccattaggca
    12121 tgatctctcc tacgcgatta gtgttcgtca cttgaacacc accttgcata aatgccagaa
    12181 tgatgaataa caggattgcc aaaataaagt tattcatggg tccagcaaag ttggtcagca
    12241 tacgctgcca caatttagcc gattggaact gtacgtcttt tggcgcaatt ctgatttctg
    12301 ttccgtcgtt ttcaataatc gtcgcatcgt gatcaacagg gtaggttact acttctgttt
    12361 cgtctccatt cacataacca gtgatagtca gctcatcttc taaatcataa cggctcaatt
    12421 ccatcgggat agcatttgtc aattggattt tcttacttaa atttatcttc tctacgatgc
    12481 cgtcagaatt tagtaaaaga gaaagaggca ttcctggtgc catttctgtt tcatcatccc
    12541 cattgcctgc cattcgaaca taaccgccaa taggtaatag gcgaagcgta tatgttgtcc
    12601 cgtcttttgc ttgatgccca taaattttcg gacccatacc gatagcaaat tcgcgaacga
    12661 ggattcctga tcgtttcgca aagaagaaat gaccaaactc atgaacaatc actaatatac
    12721 caaaaacgat gataaatgtc agaatcgttt tcatagcttt tttctccttt acttgttttt
    12781 tgtgtttttt ataaaacaac gataaaacac tataatttta ccatagtata ccaaagacac
    12841 caatagtatc aatataatga aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa
    12901 cgcaggtata cttactaaat aatgccaaat aaatgcatga taggaaatac aaacagcaaa
    12961 ctgtcgaaac ggtctaagat tccaccatgg cctggtaaga tatttcccga gtctttcaca
    13021 tcataatgac gtttgattgc agattcgacc aaatcaccaa attgtccgac tgctgaaaaa
    13081 ataatcgtca tcagaagcat gacaaacata ccatgattaa atagttcttt tgctgggtat
    13141 agcaataaat aaagaaatgc gactacaaca gcactaccaa taccaccgag cgcaccttca
    13201 atcgttttgt tgggcgaaat atcaggactt aacttatgct gaccataacg tcgaccaacc
    13261 atatatgctc cgatatcagt tgcccaaaca ataaatagac caaatatcaa aacagccaag
    13321 ctttctgttc gtgcattgac aaaattctgg aaacctattc ccacatacaa gctgacaagc
    13381 acaggaaaac ctgcttcatc aatcgtatac gtatttttag aaataactga tgttcctaaa
    13441 agaatcataa ctataagata aaataacata aatgtactta cattctctgg aagaaagaaa
    13501 aaccatcttt cttttggcaa aacaagtaac actgcaccaa cggcggataa aactccttca
    13561 aaacttaaga gtgtcaatcc tttcatccga aacagctcgt aaactcctat tactgctaag
    13621 attgctgctg taagctcaac cgctatgcct ccgatccata taatcggaat aaacaagact
    13681 agcgccacgg ctgctgtgac cacacgttgt ttcatgactg atcccctttc tcatcttctg
    13741 ttttttttac accgccgaat cgacggtctc gattttgata agaggcgatt gcttcttcaa
    13801 gatgcgcacc atcaaaatca ggccaaagcg cttttgtaaa atacagctca ctatatgcga
    13861 tctgccataa cagaaaatta ctaattcgct cttctccact tgtacggatc atcagctcag
    13921 gatcttgtaa ctctttaggc aagaaacctg tcattaaatg cttagcaatc aattcatcat
    13981 cgatttcttc tgcgtgtatt tctccttttg ctacttcttc ggcaatttct tggacagcag
    14041 tcacgatctc tgctctgctt ccataattca aagcaaaatt caataccatt ccagtattgt
    14101 tttttgtttg ctcaattgca cgtcttacag catcttgcgt atgttctggc aatacgtttt
    14161 cataacccat gacatgaact ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa
    14221 agaagtcgac aggtagttgc atgagaaaac ttacttcatc ttttggtcgc ttccagtttt
    14281 cagtcgaaaa ggcataaagc gtcaacactt tcactccaag ccttgatgct ttttttgtga
    14341 ctttctttac tgtttccatg ccttctttat ggccagcaac tcgtggcaaa cgacggtttt
    14401 gcgcccaacg accattgccg tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg
    14461 tattaaattg aaattcgctt ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta
    14521 acattctttt tcctcctata cttaaaagat tcccactatc ttaagtatta tagcaatatc
    14581 taactgtttt tcctatgaaa tcattgtttt tttatttaaa aactgaataa ttttttcttt
    14641 ttttaatttt taaataaaag aaatataaat aaaaacagag gaagcggaat gatccgcccc
    14701 tctgttttta tgattttatt aaacttccaa taattctttt tctttatcag cagcgatctt
    14761 atctacttct ttgatgctgt tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa
    14821 atcatcttcg gtaatatcac catttttttg ttgtttttta tattcatcga tcgcatcacg
    14881 acggatgtta cgaatcgcga tttttgcatt ttctgcttct tttttaacat ctttagctaa
    14941 ttcttttctg cgttcttctg tcagttgcgg gataactaag cgaatgacat ttccatcatt
    15001 ggttggactg attccgatat cacttgccat aatagctttt tcaacatctt gaagaatgct
    15061 cttgtcaaaa ggtgtgatca ttaaaacacg tgcttctgga atttggattg atgccatttg
    15121 gttcaatggt gttggcgcac cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt
    15181 cgcacggcct gcgcggattt gcccaagttc acgttgaaga ttttgtgctg cttttttcat
    15241 tttttctttt gcttctgtca taattgcatc tgccattttt atttccccct tacagttgtt
    15301 ccgatatttt cgcctaagat tgcacggcga atatttccgt gttcatttaa attgaagaca
    15361 agcagtggaa tatcattatc catgcttagt gaacttgcag ttgaatccat gacttgtaat
    15421 cctttggcaa taacttccag atgagtcagt tcttcaaatt ttactgcatt tgcatctact
    15481 ttcggatcag cagaataaac accgtctaca ttgtttttag ccatcaggat aacatctgcg
    15541 ccgatttctg ccgcgcgtaa tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta
    15601 ccgcctgcaa agataacgat ccgtcctttt tctaaatgac gttctgcttt acggcgaata
    15661 tatggctctg cgatttgacg catttcaatc gaagtttgca cgcgtgtcgg tacacccacg
    15721 ttttccaacg tatcctgtaa agctaaggca ttcataactg tagctaacat acccatgtaa
    15781 tcagcttgag cacgttccat gcccatttgt gctccgattt gtccgcgcca gatatttccg
    15841 ccgcccacaa cgattgccat ttcaacgccc agctcgtgaa cttctttgat ttcttggatg
    15901 atttctttga ttactggtgg tttgattcca aatcctgcgt ccccagctaa agcttctcca
    15961 ctcaatttta agactacacg ctgatatttc ggtgtgacca tgttcattcc tccaatattt
    16021 tctattgcca ttttaccata cttagatgat ttcgacactc taagactggt ttttcttgtt
    16081 ttgcttaaaa aaagggagcg cacaaaccgt gcgttcccgt ctgttattca acagaattat
    16141 tttttcactt ggctcataac ttcttctaca aagttatctt cgcgtttttc gataccttcg
    16201 ccaacttcga aacgtacgaa tgttttaaca gttgcgcctt ttgatgcaac gtatttttca
    16261 acagtcatat caggatcttt aacgaatggt tggtccacca aagcgatttc tgctttgaat
    16321 ttcttcaagc gtccttctac cattttttca acgatattcg ctggtttgcc ttcgttcaat
    16381 gcttgttctg taagaacagt tttttcatgt tctaattcag cttctggaat ttgtgattcg
    16441 tttacataac gaggattgat agctgctacg tgcatagcta cgtctcttgc aactgtttcg
    16501 tcagttgttc catctagaac agtcaatacg gcaatacgtc cgcccatatg caagtatcca
    16561 ccaaatgcag cattgtcgtc tttttctaca acttcaaagc gacggaagct gattttttct
    16621 ccgataactt gagttgcttc gatcaagtca gattcaattg tacctttatc tgttttgatt
    16681 ttcattgcag cttccatgtc tgctggtttg ttttcagcaa caagttctgc aatttctttt
    16741 actaaatctt ggaacatttc gtttttagaa acaaagtctg tttctgagtt tacttcaacg
    16801 attgctgcag tgttaccttt cacagcaact gaagccaatc cttcagctgc aatgcggtca
    16861 tttttcttcg cagcctttgc cattcctttt tcacgtaaaa gatcaactgc tttttccatg
    16921 tcgccttcta cttcaactaa tgcttttttc gcatccatca ttcctacgcc agtcatttcg
    16981 cgtagttctt ttaccatttt agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt
    17041 ttctttttaa aaaaagctgc ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca
    17101 gctccatcat tcaaaattat tctgctgaag aattgtcgcc ttcaacaaca tctacgattt
    17161 cttcgattga tgttgctgtg ttttcttctg tgaaatcttc ttcaactact tgatcttcac
    17221 cttggtttcc ttcgatgaaa gcatcagcca tttttgaagt aatcaattta acggcacgaa
    17281 ttgcatcgtc gtttgaaggg attactacgt cgatttcatc tggatcgcag tttgtatcaa
    17341 ccatagctac gattgggata tttaatttgt gtgcttcttg aacagcaata cgttctttac
    17401 gtgggtcaac gatgtacatt acatctggga ttcttggcat atcagcgata ccgcctaaga
    17461 atttttcaag acgttcacgt tctttgttca aaccagcaac ttctttttta ggtagaactt
    17521 caaaagttcc atcttcttcc attgcattga tttgtttcaa acggctgata cgtttttgga
    17581 tagtatccca gtttgtcaat gttccgccta accaacgatg gttaacgaag tattgtcctg
    17641 cacgagttgc ttcttctttg attgcttctt gtgcttgttt ttttgtacct acgaataatg
    17701 ctacgccgcc ttcttcagca acgctcttca tgtaatcata agctgcatct actaatttaa
    17761 ctgttttttg caagtcaatg atgtagattc cgtttctttc tgtgaagata tatttcttca
    17821 tttttggatt ccagcgacga gtttggtgac caaagtgtac gccggcttct agtaattgtt
    17881 tcatagaaat tactgccatg tttgtttcct ccgatttggt tttatttttc ctcttcttat
    17941 cttctacctt gccggcaaac tttttcaagc acctaccgac aatccaataa gaatgtggat
    18001 ttgctgatgc ataaagcacc ctcagctatt atacagacaa aagatccgta tcgcaagagc
    18061 aaatatactt ttctgctcaa cattcaatag tttacctgaa agataaaaag aattcaagaa
    18121 aaacagtaga tttctctttg tttctttatt atcctttttc cataaaataa ttttaggtat
    18181 aaaagacgaa tattattggg caatccttct tttttttgtt atgatacatg tgaaatcttt
    18241 cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt gctgattaaa caagtcattg
    18301 aagaattccc gatccgtacg caagaagaac tattaaacaa acttgggcat tatggcgtta
    18361 ccgccacgca ggcaacgatt tctcgagata tccgtgatct aaaaatcgtg aaggcacctg
    18421 atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa aggaactcct gagactagga
    18481 ctgaggaaga ggatcggctg gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag
    18541 tccacttttt gacgatcgtc aataccttgc cagacaatgc tcatttattt gcatctgttt
    18601 tagatgagat caaacctccc tctattgtca gtacaattgc tggattcgat acgatcatgg
    18661 tcatctcgga aaatgaggag gacgcaaaac gagtagaaga atttcttcac catccaactg
    18721 aagtatcact tttttaatca gagcacgtat tcctaatatg gaatgcgtgt ttttttattt
    18781 tcaatatttt ccttattgaa taaaccttta cactctttac aaaaatacat cattttttat
    18841 tcaaattttt caacaccttg attgaatcac aataatcatc atttatattg gatgttaaca
    18901 agagcagctt cttgctcttt gtgtatttca aaaaagaaat gaaaggaaat catatgtata
    18961 aattattaac ttctggacca ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg
    19021 atcattgtac ttgggatcaa gaatacaaag aaattacaca atggataaga aaaaaattat
    19081 tggatcttgc tcaagtatcg gaaggaatat acacaacggt cttaatgcaa ggtagcagct
    19141 ccttcggggt agaatcggta ttaagttctg tgatcaggcc agaagatact gtactgattt
    19201 gttcaaacgg cgcatataga caacgaatga tcaacatgtg cgaatgtctg aaactgaact
    19261 ataccgttta tgaggtatcg gaacatcaaa tcctccaagc tcatgtgatt acacagctga
    19321 ttgaagagaa tccgtctatc tccgcactag caatggtcca tagcgagact acttccggta
    19381 ttttgaataa tttggaagag attgctgctg tcacacaaga aagagatctt ttgtttatag
    19441 tggacgcgat gtcaagcttt ggtgggatac cgatttgtgt agccgaacta gggattgatt
    19501 ttttgatttc cagctcaaac aaatgtattc aaggtgttcc aggcttttct tttgtacttg
    19561 ctaataaagc ggaactggaa aaacgaaaaa atgttagccg aagtttgtca cttgatctat
    19621 atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg gcgtttcact tcccctaccc
    19681 atacggtttt ggcttttcaa caagcgctga aggaactaga ggcagaagga ggaatcgaaa
    19741 ctcgatattc acgttatctc gccaataatc atgtgctacg acaaaaaatg acagagatgg
    19801 ggttccagcc tttcattcaa gaatatcagg ggccattcat cacctctttt ctttacccta
    19861 aggactggcg atttgatttt aatcattttt atcagtattt aaagcaaaat ggatttgtga
    19921 tctatcccgg gaaaatcagc gatcatgaat gttttcgtat cggaaatatt ggcgagatct
    19981 atccggaaga tattttggaa ctgacagcaa ttatcaaaga ctataaggag aacgttaatg
    20041 attaaaactg taatttttga ttgggcagga acaactgtgg actttggctg tatggctcct
    20101 gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc aactgactga caaagaaatc
    20161 cgctaaccga tgggaaaatt aaaatgggat catatccaac agcttctttc cctcccttct
    20221 gtcaaaaaac aatggatagc tatttatgga caactacctc aggaaaaaga ggtggaggaa
    20281 ttgtatcaac aatttgaaca ttatctgttt aaagatttgg tcaagcattc taccttgaaa
    20341 cccgacacat tggaaacgat cgaacggttg agaaacgctg ggatcaatat tggttccacg
    20401 aatggattta atgctcctat gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat
    20461 tcaccagact tcatggcaac ttttgaagac gtcgaagggt atggtcgacc gtttccttat
    20521 atgatccata aaaacatgca gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc
    20581 ggagatacgg tctcagatat ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta
    20641 attgaaggta gttcgctaat ggggatttct tatgatgagt accaaaaatt agagatgggg
    20701 aaaaagatcg tttaaaacag tctgttgctc ttagattcca acaagccggc gctgatattt
    20761 gtgtaaacaa tttaaaagag ttaacagaat acattttgta cgctgacatg aatccagaat
    20821 gacttttata caaaataaga ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag
    20881 ccaagcaaaa atagctcctc atatttttcg tttggttagg cttattaccg cagatgcaag
    20941 cttttgaaca ccgtttattc gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt
    21001 ccccttattt gcaaacaaac agtgtttaag agacaacctc tccactaaac ggtgttcatt
    21061 cagctgactc tcggtatttg ctcaaaaaac gataaaattt gaaaagctat tttctctttt
    21121 gtttctctaa tactcggatc agaacgctga tttcacattc tctttttctt acatcttatc
    21181 tggtgcatga agtcctagca aacgaaggtc ttctttcaat aatacagtca ctgcataaac
    21241 tagagctaag cgtgcttctt tttgttcatc atcagctaaa atcttcgtat gagcatagta
    21301 tttgttgaat gcttgtgcta atttgattgc atgtttcgca atcactgacg gttcgtattt
    21361 ttccccagca gacaagaccg tttcagggta tttttgaact aatttaacca cttcccagct
    21421 ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg acaaatcctg ctttttccaa
    21481 caggctgact gcacgagcat gtgtatattg cacataaggt ccagtttctc cttcaaatcg
    21541 aacaacttct tccaggttaa agtcaaatgt attcagtcga tcgtttttca aatcgtggaa
    21601 aatgactgcc ccaaccccaa cttgtttagc tacagcatct ttgttttcca aatcagggtt
    21661 tttctcgctg atttgttctt tagcagattg gattgcttca tttaatactt cttctaataa
    21721 gacgatcttt cctttacgag ttgataattt tttaccacct tgcgtgatca aaccaaatgg
    21781 aatatggtgc atgtcgtcag accagtcaaa gcccatttcc ttcaagacag ctttcaactg
    21841 tttaaaatga tagctttgtt cattccctac tacatataga gattgtttga agtcgtaagt
    21901 acgtttacgg tacaatgcag cagctaagtc acgtgtaatg tatagtgtcg caccgtctga
    21961 cttcttaatc aacgctggat tcaggtcata agcagatagg tcaacgatct ctgcaccttt
    22021 atcctcgttc agcaaatgtt tttcttctaa aagtttgacg atttcatcca tcttgtcatt
    22081 gtagaaagct tcaccgttca acgaatcgaa acgtacttcc agcagatcat agattttatt
    22141 gaattctttc atcgattcat cacggaacca ttgccataat tgaatagcct ctttatcgcc
    22201 ttcttccaga cgtttgaacc aagcacgagc ttcttcattt aattctggtt ctgtttcagc
    22261 tacttcatgg aattggacgt ataaacgaag caattcattg attggttctg ctttaacagc
    22321 ttcttctgta ccccattttt tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc
    22381 acctaaatgg ttgatgcgga ttggctgata accgattttt tccataatga aaccgatcga
    22441 attaccgata actgtcgaac gcaaatgtcc cattgagatt ggtttggcaa tattaggaga
    22501 agacatgtcg attggtactg ttccttgatt cccgatgttg ctatccccat aatgttcttt
    22561 ttctttcact actgtttgca aaactttttt gctgataagt tctttattca tgaaaaagtt
    22621 caagtaaggt ccgacaactt cgattttttc aaagttggca gaatcgattt tttctgctaa
    22681 atctgcagcg atttgctgtg gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa
    22741 tgcaacatct ccgtgttcag ctgattttgg attttctagt aattgctcga cttgttccag
    22801 tgttaaatca tctttgacag cgtcatagac agcttttgcg acaagatctt tattattcat
    22861 ctttattcct cctaaagtgt tcccttgatt gttaaacaaa aaaaatctct agcgacggca
    22921 tacttagtat gccatcacta gagacgagat ttcccgcggt accactccaa ttgtcctaaa
    22981 aaaggaccag ctccatctga tatcggtaga cgaccgtctg tttaaaacag aattctccaa
    23041 gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact tccgaattcg ctagccaaga
    23101 atgatacaag tactctcttg ttcaacaaat cttagtctta ttaaactttc ttgaattata
    23161 tcagaaaaaa aaacgaaagg ctagtcgttt cataaaaaaa caatccagat ggcctaaagg
    23221 agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga cttaacaaat
    23281 taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat attttctaaa
    23341 gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc tgcttctact
    23401 ttcggaagca tgcttcctgg agcgaattgc ttttcatcaa tgtattgttt catttctgaa
    23461 acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata cacattgtct
    23521 actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt ttctgaagca
    23581 aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc tacaacagga
    23641 atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt ttcgatgaca
    23701 cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca tccacgtcca
    23761 gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc ttcttcttcc
    23821 gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt ttcatccaca
    23881 ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt cagtacttcg
    23941 cccatggcgt tttgtaacca gtaaccgata gatccttctg tcattgcaac acatgtatct
    24001 aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa taggttaccg
    24061 acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat aaatttcact
    24121 aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc atccgtagac
    24181 aagatggcat tgccccctaa agcgacaact acttttcgtt tggccatttc attcgtcctt
    24241 tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt gattttccct
    24301 caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg agggataaac
    24361 aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt ttctgcttgg
    24421 tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat ttcagtgatg
    24481 ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa agctggcaag
    24541 cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc catattgatt
    24601 tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc cattgatacc
    24661 catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc gtcggtaatc
    24721 atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat ttcttcttca
    24781 ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc acctgtgact
    24841 aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt gataccttct
    24901 actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt tggatgccat
    24961 tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc ctctaccatt
    25021 ttttgactga atccacggaa ttcgattccg tcaaacatac gtcctagtac tttagctgta
    25081 tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata ttcaggatgt
    25141 gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga tgttttttca
    25201 aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg ttttttcaag
    25261 tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt aaagtctttt
    25321 tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat tctcctttag
    25381 attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta agactgccgt
    25441 caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca gcgtggacca
    25501 ccacgacctc gtgacaattc acttgaattg atttctaaca ctttgatgcc atagctacga
    25561 agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg agcgattgcc
    25621 aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc tccattacca
    25681 gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc gtgtaagtta
    25741 tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac atccatctta
    25801 ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac cattgtaaat
    25861 acggtatcca aatgcatcat tgcacggttg ttcggaattt tgattgccaa cactttttca
    25921 aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt cgcattagtt
    25981 cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc tccgccttcg
    26041 atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc aaatcttgga
    26101 tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc
    26161 ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt
    26221 ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcata tagactgcta
    26281 gaacgtactt cgatatcttt tgtacgaacc ccagccatga ttttatctac catttcttgc
    26341 gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga tgcaatatga
    26401 gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc aatcgcttct
    26461 gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt cttcgcaaag
    26521 ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa cagacgatcc
    26581 ataatatctg gtgtcaaatt ttccacttct tgtccaggcc gtttcagtaa aactgtcttt
    26641 aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg cctccttctt
    26701 tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt tgtctcattc
    26761 aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt ttttctattt
    26821 ttaagcgtaa tttattcatt tttt
//
LOCUS       DAZKSR010000034        26160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000034 DAZKSR010000000
VERSION     DAZKSR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.034"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8XG_001261"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8XG_001261"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0773187.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACG8XG_001262"
     CDS             1286..2461
                     /locus_tag="ACG8XG_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0773188.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACG8XG_001263"
     CDS             2465..3106
                     /locus_tag="ACG8XG_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773189.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACG8XG_001264"
     CDS             3103..4020
                     /locus_tag="ACG8XG_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0773190.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACG8XG_001265"
     CDS             4017..4688
                     /locus_tag="ACG8XG_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773191.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACG8XG_001266"
     CDS             complement(5229..5717)
                     /locus_tag="ACG8XG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0773192.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACG8XG_001267"
     CDS             complement(5918..6184)
                     /locus_tag="ACG8XG_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0773193.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACG8XG_001268"
     CDS             complement(6184..6447)
                     /locus_tag="ACG8XG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0773194.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACG8XG_001269"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACG8XG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACG8XG_001270"
     CDS             complement(7250..9073)
                     /locus_tag="ACG8XG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0773195.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACG8XG_001271"
     CDS             complement(9671..12376)
                     /locus_tag="ACG8XG_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0773196.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACG8XG_001272"
     CDS             complement(12682..13629)
                     /locus_tag="ACG8XG_001272"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0773197.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8XG_001273"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8XG_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0773198.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACG8XG_001274"
     CDS             complement(14264..14626)
                     /locus_tag="ACG8XG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0773199.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8XG_001275"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8XG_001275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0773200.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8XG_001276"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8XG_001276"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0773201.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACG8XG_001277"
     CDS             complement(16254..17765)
                     /locus_tag="ACG8XG_001277"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0773202.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8XG_001278"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8XG_001278"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0773203.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACG8XG_001279"
     CDS             complement(19592..20668)
                     /locus_tag="ACG8XG_001279"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0773204.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACG8XG_001280"
     CDS             20864..22609
                     /locus_tag="ACG8XG_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0773205.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACG8XG_001281"
     CDS             complement(22889..23326)
                     /locus_tag="ACG8XG_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0773206.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACG8XG_001282"
     CDS             complement(23310..24683)
                     /locus_tag="ACG8XG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773207.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACG8XG_001283"
     CDS             complement(25005..25493)
                     /locus_tag="ACG8XG_001283"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0773208.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACG8XG_001284"
     CDS             complement(25515..26117)
                     /locus_tag="ACG8XG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0773209.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DAZKSR010000035        25536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000035 DAZKSR010000000
VERSION     DAZKSR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.035"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8XG_001285"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8XG_001285"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0773210.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG8XG_001286"
     CDS             complement(937..1518)
                     /locus_tag="ACG8XG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0773211.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8XG_001287"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8XG_001287"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0773212.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8XG_001288"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8XG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0773213.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8XG_001289"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8XG_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0773214.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8XG_001290"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8XG_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0773215.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8XG_001291"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8XG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0773216.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8XG_001292"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8XG_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0773217.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8XG_001293"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8XG_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0773218.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8XG_001294"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8XG_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0773219.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8XG_001295"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8XG_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0773220.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8XG_001296"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8XG_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0773221.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8XG_001297"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8XG_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0773222.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8XG_001298"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8XG_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0773223.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8XG_001299"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8XG_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0773224.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8XG_001300"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8XG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0773225.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8XG_001301"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8XG_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0773226.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8XG_001302"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8XG_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0773227.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8XG_001303"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8XG_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0773228.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8XG_001304"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8XG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0773229.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8XG_001305"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8XG_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0773230.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG8XG_001306"
     CDS             13972..14157
                     /locus_tag="ACG8XG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0773231.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8XG_001307"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8XG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0773232.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8XG_001308"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8XG_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0773233.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8XG_001309"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8XG_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0773234.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8XG_001310"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8XG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0773235.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8XG_001311"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8XG_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0773236.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8XG_001312"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8XG_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0773237.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG8XG_001313"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG8XG_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0773238.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG8XG_001314"
     CDS             18232..18879
                     /locus_tag="ACG8XG_001314"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0773239.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG8XG_001315"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG8XG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0773240.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8XG_001316"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8XG_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0773241.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8XG_001317"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8XG_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0773242.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8XG_001318"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8XG_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0773243.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG8XG_001319"
     CDS             20317..21255
                     /locus_tag="ACG8XG_001319"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0773244.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8XG_001320"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8XG_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0773245.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG8XG_001321"
     CDS             21935..22774
                     /locus_tag="ACG8XG_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0773246.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG8XG_001322"
     CDS             22750..23619
                     /locus_tag="ACG8XG_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0773247.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG8XG_001323"
     CDS             23609..24406
                     /locus_tag="ACG8XG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0773248.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG8XG_001324"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG8XG_001324"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0773249.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5497 g         7028 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctc
//
LOCUS       DAZKSR010000036        25299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000036 DAZKSR010000000
VERSION     DAZKSR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.036"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..614
                     /locus_tag="ACG8XG_001325"
     CDS             <1..614
                     /locus_tag="ACG8XG_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0773250.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            611..1384
                     /locus_tag="ACG8XG_001326"
     CDS             611..1384
                     /locus_tag="ACG8XG_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0773251.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1381..1779
                     /locus_tag="ACG8XG_001327"
     CDS             1381..1779
                     /locus_tag="ACG8XG_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0773252.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1964..2635
                     /locus_tag="ACG8XG_001328"
     CDS             1964..2635
                     /locus_tag="ACG8XG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773253.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2694..3134
                     /locus_tag="ACG8XG_001329"
     CDS             2694..3134
                     /locus_tag="ACG8XG_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773254.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3146..3814
                     /locus_tag="ACG8XG_001330"
     CDS             3146..3814
                     /locus_tag="ACG8XG_001330"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0773255.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3878..4501
                     /locus_tag="ACG8XG_001331"
     CDS             3878..4501
                     /locus_tag="ACG8XG_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0773256.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4557..4943
                     /locus_tag="ACG8XG_001332"
     CDS             4557..4943
                     /locus_tag="ACG8XG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0773257.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5156..6547
                     /locus_tag="ACG8XG_001333"
     CDS             5156..6547
                     /locus_tag="ACG8XG_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0773258.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            6894..7541
                     /locus_tag="ACG8XG_001334"
     CDS             6894..7541
                     /locus_tag="ACG8XG_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0773259.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    7670..7886
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7934..10579
                     /locus_tag="ACG8XG_001335"
     CDS             7934..10579
                     /locus_tag="ACG8XG_001335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0773260.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            10889..12202
                     /locus_tag="ACG8XG_001336"
     CDS             10889..12202
                     /locus_tag="ACG8XG_001336"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0773261.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            12192..12845
                     /locus_tag="ACG8XG_001337"
     CDS             12192..12845
                     /locus_tag="ACG8XG_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0773262.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            12900..13592
                     /gene="radC"
                     /locus_tag="ACG8XG_001338"
     CDS             12900..13592
                     /gene="radC"
                     /locus_tag="ACG8XG_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0773263.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            13742..13942
                     /locus_tag="ACG8XG_001339"
     CDS             13742..13942
                     /locus_tag="ACG8XG_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0773264.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14196..15875)
                     /locus_tag="ACG8XG_001340"
     CDS             complement(14196..15875)
                     /locus_tag="ACG8XG_001340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0773265.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15877..16089)
                     /locus_tag="ACG8XG_001341"
     CDS             complement(15877..16089)
                     /locus_tag="ACG8XG_001341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0773266.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16482..18212
                     /gene="efrA"
                     /locus_tag="ACG8XG_001342"
     CDS             16482..18212
                     /gene="efrA"
                     /locus_tag="ACG8XG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0773267.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18209..19969
                     /gene="efrB"
                     /locus_tag="ACG8XG_001343"
     CDS             18209..19969
                     /gene="efrB"
                     /locus_tag="ACG8XG_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0773268.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            20064..20261
                     /locus_tag="ACG8XG_001344"
     CDS             20064..20261
                     /locus_tag="ACG8XG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773269.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20414..20899
                     /locus_tag="ACG8XG_001345"
     CDS             20414..20899
                     /locus_tag="ACG8XG_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0773270.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(21003..21596)
                     /gene="clpP"
                     /locus_tag="ACG8XG_001346"
     CDS             complement(21003..21596)
                     /gene="clpP"
                     /locus_tag="ACG8XG_001346"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0773271.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21776..22303
                     /locus_tag="ACG8XG_001347"
     CDS             21776..22303
                     /locus_tag="ACG8XG_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773272.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22394..23131
                     /locus_tag="ACG8XG_001348"
     CDS             22394..23131
                     /locus_tag="ACG8XG_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0773273.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23135..24052
                     /gene="miaA"
                     /locus_tag="ACG8XG_001349"
     CDS             23135..24052
                     /gene="miaA"
                     /locus_tag="ACG8XG_001349"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0773274.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            24054..25283
                     /gene="hflX"
                     /locus_tag="ACG8XG_001350"
     CDS             24054..25283
                     /gene="hflX"
                     /locus_tag="ACG8XG_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0773275.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8149 a         4304 c         5529 g         7317 t
ORIGIN      
        1 ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt gcggcaatca
       61 tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg gaagtcggag
      121 atctagtagg gaaatatatg atcaaaaaga aaagtttgac cgttttggtc agtttgggat
      181 ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg ggacaacaag
      241 tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc gtcagcgtcg
      301 gcactggagt atttctagcc tttgcgctgc ttcgggtcgt attcaagcta tcctattatc
      361 aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc agttccgaat
      421 ttatgccgat tgcttttgat tcggggggag tgactactgg tccagtaact gttccattta
      481 tcttggccct tgccggaggg atgactagta tgatcaaaca aaagaaaaat gaaaatgaca
      541 gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg attttggggg
      601 tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg ttaccatgga
      661 agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata tcattagttt
      721 tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt ttcttgataa cgactattgg
      781 gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac agcatttagg
      841 cagtgctatt gcgggattgc cttacaatta tatactggtt ccccttggct ttttaatggg
      901 gttcttagta gggtttgctg agccggcgat tcatgtaatg atcaaacagg ttgaagaatt
      961 gagcgagggg agaatccgat cgaaaatcat gctgatggta atttcaattg ggatcggcgc
     1021 agcagtagga ttgtctatgt ggcgtttgct cgtcggattc tctctttatt atttcttgat
     1081 cccaggatac gggctagcat ttattttagg acgaaaagtg gacaagatct ttttagcaat
     1141 ggcttttgat aacggtggag tagcaacggg cccgatgtgt tcgacattta ttttatcaat
     1201 gagcgtggca atcgctgcac aaattgaagg aagagatccg atcattgacg ggtttggtgt
     1261 ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat atatttataa
     1321 acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg gggaaagcaa
     1381 atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga cggaggaatg
     1441 ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac gatcttacat
     1501 ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat ttcgccagaa
     1561 aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat ggatgcgatt
     1621 ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat tgatgtagac
     1681 aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat gcaagaactt
     1741 ttagatgaaa ataacgatag agaaaagtct gaatcataga caagcgtttg aagaggaaat
     1801 aaacacaaac aagaaggcgg atgagcgtta tttccgcttt tttgtttgta tttttctcgt
     1861 gaatacttat gaaatagtaa atttttatga aattctactc taagcatcag actttctttt
     1921 caaaatatgc tattttaaaa atgatgaaaa aagggggaga aaaatgaatt ttttagttgt
     1981 tttaaaagat actctgattg agcgatttac atttctatat gttcaattgc aggaaaataa
     2041 aacattgctg catttttcac cagagttcca tctagagggg ctaacattag gagaaatcta
     2101 tcaagcagaa gggctgtcgg ctgttttgca cttttttaag gtagaattgg aattaccagt
     2161 aaaagattat atcgagcttt ctcttgaatc ttgggatcga gcagtggttg aactgtttcc
     2221 agaaggttta atgatcgata caaacgatgg gaagatccat ctgactgcag cagaactgca
     2281 aaaacaaatg cgttatcaaa tagatgacga aaattctttt tctattttta gacgtcagca
     2341 aaagattcta aaaagctttt taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac
     2401 acagctgttg aaaaaatatc caggtctgct tcacacgtct attcaatttc ctcaattgat
     2461 tacgatgatc catcgtttta tacgtatgca acagctacca tctaagaagc tgtttcttcc
     2521 attgacggac acatttcgag tagtcaacag agaagataaa aagaaagtga ttgtgattga
     2581 ctttaccaga aatcggaatg tccttcacca agttgcaatg gaaaaggcca attaaggctt
     2641 tttctttttg gatttttttg tcataataaa tattagatga tggaggagat aatatgatgc
     2701 aaacatggtt agaaaggcta atacgatttc agcagaacta tcacaaactt atgcagggaa
     2761 gatatgcgaa atttgatcag ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt
     2821 tgctaagtcg ttggttgccc tatagaatcg gacaattgtt gttcatcgtc tttttcggat
     2881 ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt
     2941 atttgaaata tcttgataga gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag
     3001 tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc gaattgtcat caaaagcagc
     3061 gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg
     3121 aaacgaatgt ttaagggaga aaagaatgaa attagtatta ttacgtcatg gcgaaagcga
     3181 agcaaatttt gaaaattact ggacaggatg gctagatgtc gccttaacag aaaaaggaaa
     3241 agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt
     3301 ttatacctct gttttgaaac gtgcgattct taccggacaa gtcgcactag aagaaatggg
     3361 tcagttatgg cttcctataa taaaaacatg gcgtctaaat gaacggcatt atggggatct
     3421 tgtgggtaaa aacaaagata agatgaaaga aatatacgga aaagatcaag taaaaacatg
     3481 gcgcagagga tatacggaag ttccgccgtt aaccgcagaa aatagatttg accgcagata
     3541 tgatcacttg gatccgcatt tgatcccata cggagaaagt ctggaaatga cagtgaatcg
     3601 gatcattccc ttatggcaag atcatctagc accaaaacta aaagatcggc aagatttact
     3661 agttgtgggt catggtaata gcatccgagc actgacaaaa tatttagaag atgttcctga
     3721 agatcaaatg gatacgatcg atatcccaaa tgcacaacca atccagtata cattagcaga
     3781 tgatttgaca attctggaca aaaaaatact ttaaagaaca cctttatcta aaagtgagag
     3841 atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg aaaaattata tatgggactt
     3901 tgatggtaca ttgtttgata cgtatcccgc aatggtagat ggtgcatggc aagcactaaa
     3961 agattttggt atcagtatgg acaaaaaaga aatctacttc aaaatgaaaa agtattctac
     4021 tagttatttg atcaatgaat caaacttgaa tgccagagag tttaatgaat tatttcaccg
     4081 atatgaaaaa gaaagtacgg aagtttcccg tccatttcca gaaacgaaac aagtactgga
     4141 aatgctaaaa gacaacggcg gtcgtcattt catcctgacc caccgtttga cagaatcaac
     4201 atggggcctg ttaaaagaac atcgattggc acatctgatt gaggaagtag tagggatcga
     4261 tcaggacttt cctcgtaaac ctgatccagc atctttaaat tatttgatcg atacttttca
     4321 tttagaaaga acagacacga tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa
     4381 aaatgcagga gtcgctactt gtctttacga catcgatcat tttctgggtg agatcccggc
     4441 agattatgtg gtaggaaatc tgaatgaaat ccttaccttg cagagcatca ttaaaaagta
     4501 gtatcccctt tcaaaggaga tatgttataa ttttcataaa ggagtgataa aattatatgg
     4561 aaatcagaag acaaaaagaa gttgtagaag catttcatta tgatatgaga ttgccgaacc
     4621 aagaagtaga aacagatttg aaagtaggct tctcgccttt aaaagaaacg atcgaaaact
     4681 atccgaaaga acatagcatc gtaggagcac gcttagagtt ccgtttagtg tttgatacat
     4741 atgttttatc tggaagtgtg agtcagatca atcatatcct caatcggaaa attgaaggtc
     4801 aaaatgatgt cacacaagaa gaagtggacg agctagtagc accgttattc gatatcgtac
     4861 gccgaatgtc ttatgaggtg acagagatcg ccttagatca gcctggggta aaattgaatt
     4921 ttcaatcaga aaaacaagaa tagacagtaa aaacatacct gcgatagatt aaagctatca
     4981 caggtatgtt tttttattac taattgtaag caaaaagaat gtcatttcga taaaaatcgt
     5041 ctaaaatata aaagtcactg aaaaaaattt tttcaggagg atggttgaag aatcattctc
     5101 gggaaccttt acttccttcg atggacttgt cattaataat atgaggagga gaaaaatgac
     5161 aacactcatc acagttttag tggttctagt ttttgcggca ttattatatg tgttctatcg
     5221 tatgcaaaag cgccatacaa aattttcaac acgcgtcttt acagcattag gagttggtat
     5281 cgtctttggc ggtctgcttc aattgatctt tggtgtagac gacaaagtcg ttactcaatc
     5341 aatggaatgg atcgggattg taggtcaagg atatgtatca ttcttgcaaa tgctagttat
     5401 gcctttagtg tttatttcga tcattggtgc attcacaaaa atgaaagttt ctgaaaagct
     5461 agggaaaatt agtgcgactg tattgacttc tttacttgga acaacagcta ttgcagcatt
     5521 gatcggaatt ggttcaacaa tgctgttcgg actccaagga gctaaattca cacaaggagc
     5581 tgcagaaaca gcacgaatct ctgaactggc aacacgtcag gaaacagttg aaaacttatc
     5641 gattccacag cagattttat cctttatccc taaaaacgtt tttgctgatt tagcaggtag
     5701 tcgtgcaaca agtacgattg gagtcgttat tttcgcggca tttgtcggaa tcgcttatct
     5761 aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt tcagatttag tcgacagtct
     5821 gtataaaatc attatgcgga tcgtgacgct tgttttgcgt ttgacacctt atggagtatt
     5881 tgctttgatg acaaacgttt tggcaacaag tgattttgca gctatcgtca atttaggtaa
     5941 atttgtcgtt gcttcttacg tagcattgct tgctgttttg attgtccact cactgatttt
     6001 gattggtgca aaagtcaatc caatcaacta tttcaaaaaa gcctttccgg tattgagctt
     6061 tgcgtttact tcacgttcaa gtgctggagc tttgccattg aatatcgaga ctcagacaaa
     6121 agctttagga gtagatgatg caacagctaa tttctcagca agttttggtt tgtctattgg
     6181 acaaaacggc tgtgctggcg tttatccagc tatgttggca acgatcgtcg caccagcagc
     6241 tggagtgaat gtctttagtt tggaatttat tttgatgctt gtggcaattg taacaatcag
     6301 ttcttttggt gtagctggag tcggtggggg cgcaacattt gcttcactga tcgttttagg
     6361 ggcaatgaac ttgccagtag caatcgttgg gttagttatc tctgttgaac cattaatcga
     6421 tatggcacgt acagcagtaa acgtcagcgg tagcatggta gctggtatcg tgacaagtgc
     6481 acgcatcaaa gatttagatc gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa
     6541 tgcatagtca aaaagaggtt gtgatagaag tggtcagctt taagaaataa gaagaagttt
     6601 cctaaaaata gtttctcaaa tttttgtgaa atttcggctt atttccgaga agctacttct
     6661 gaaacaccgt gaataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     6721 aaacaaaaat agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct
     6781 tttgaacacc gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg
     6841 tgttagaaac atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag
     6901 aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact
     6961 tgaagatgct ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca
     7021 cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa
     7081 gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta
     7141 aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca
     7201 acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac
     7261 tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag
     7321 ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg
     7381 ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag
     7441 taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc
     7501 ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta
     7561 ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg
     7621 ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct
     7681 gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc
     7741 tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac
     7801 gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg
     7861 taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa
     7921 ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt
     7981 tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa
     8041 aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt
     8101 agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg
     8161 atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt
     8221 agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat
     8281 cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa
     8341 aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga
     8401 agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata
     8461 tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa
     8521 agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt
     8581 agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc
     8641 agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa
     8701 acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa
     8761 aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg
     8821 cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat
     8881 ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat
     8941 taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga
     9001 acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga
     9061 taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt
     9121 acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca
     9181 aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc
     9241 ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt
     9301 gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt
     9361 ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat
     9421 tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt
     9481 gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat
     9541 ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc
     9601 accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa
     9661 caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga
     9721 gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga
     9781 aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca
     9841 actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt
     9901 actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact
     9961 tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga
    10021 acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga
    10081 gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt
    10141 tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa
    10201 aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt
    10261 ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc
    10321 agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga
    10381 agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca
    10441 aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga
    10501 gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag
    10561 tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa
    10621 aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt
    10681 cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta
    10741 ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc
    10801 ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta
    10861 taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca
    10921 tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt
    10981 ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg
    11041 ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt
    11101 tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc
    11161 agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca
    11221 atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta
    11281 ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga
    11341 ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac
    11401 cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca
    11461 aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac
    11521 agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct
    11581 gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc
    11641 tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga
    11701 attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa
    11761 gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt
    11821 tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa
    11881 agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag
    11941 agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga
    12001 acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat
    12061 cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt
    12121 attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt
    12181 aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca
    12241 cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg
    12301 ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc
    12361 atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc
    12421 aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac
    12481 tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag
    12541 cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt
    12601 ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga
    12661 agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag
    12721 cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag
    12781 aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa
    12841 aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt
    12901 tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa
    12961 tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc
    13021 aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg
    13081 cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga
    13141 aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa
    13201 gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga
    13261 aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct
    13321 taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt
    13381 tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg
    13441 gtaatgttat tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag
    13501 aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt
    13561 tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca
    13621 agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc
    13681 tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat
    13741 tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg
    13801 tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac
    13861 attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc
    13921 tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt
    13981 ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg
    14041 caggtatttt tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg
    14101 tatcaaggaa taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat
    14161 attcaggaca aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc
    14221 attggcaaaa tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca
    14281 gtcatcgctt ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa
    14341 gcgtggaata agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca
    14401 aatccacgag agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc
    14461 gcgacagcta atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta
    14521 ccaatatcac cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca
    14581 ggacgcgcag aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt
    14641 tctgcaggaa cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct
    14701 ccgtggacag gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct
    14761 tgtccaccgt gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca
    14821 gcttcagaca gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa
    14881 cttgaaaaga tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg
    14941 atgcggctca acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca
    15001 ttggcaggca agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata
    15061 tatcctaaac gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt
    15121 ttacgaccag ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca
    15181 aagctggcaa aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca
    15241 atcgtttttt ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg
    15301 cacaagactc cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca
    15361 ggagtgaaat cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc
    15421 acgatcccta atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt
    15481 ctaaaacgga tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca
    15541 tgctcatcta atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata
    15601 tttgcttgtt tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc
    15661 accaaggctt ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag
    15721 tcaattccaa gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca
    15781 tcttggaatt gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag
    15841 acgcctgtct cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc
    15901 agcattttct ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt
    15961 gtatggggta ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta
    16021 aagagatttt gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt
    16081 atagatcatt gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt
    16141 ttagacaaca caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata
    16201 ctggattcga gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa
    16261 ccgtaccttc tgtataaaaa atatacaaca attaattgtt attctagcat aaaacagcaa
    16321 ataagtatag aaatcaacta gattgaaaga aaaaatcaat gtttatcatc ccgctataat
    16381 cataatgact tttttatgag agaagtgttc tattaatagg ctcgttgtgc tatcattttt
    16441 agtaatgaaa atgtaacaaa agattaaaag gagatgagaa agtgaaacta atgtggcatt
    16501 ataccatgcg ttataagaaa ttcttgtttt tcaatttcat atgcgtattt ggttttattt
    16561 tgattgaatt aggacttcct acgattttag cacgtatgat cgatgtagga atccgcaatg
    16621 atgattttga ttatgtgaag caacaaggga tatttatgat cgttattact gtaatcggta
    16681 ttattttgaa tatcttgtta ggttattttg gttcgaggat tacgactaat atcgtagcag
    16741 atattcgaga tgatttgttc gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc
    16801 taggcgtatc ttcattgatc actcgtacaa caaacgatgc ttatcagatc atgttgttcc
    16861 tgcaaaatat tttacgtatc ggttttatgg caccaatgat gttcattgtc agtttgtaca
    16921 tggtcatgcg tacaagtgtg acactcagtt tgtatgtagt aggtgcatta cctttactat
    16981 tattggcagt cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa
    17041 acttagataa aatcaacagt atcttgcggg aaaatctttc aggattgcgt gtgattcgtg
    17101 cattcgtcaa tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag aattatgctt
    17161 ctagttcaaa aagcttattc cgtttgatgg ctgttgcaca accgggtttt tatttcttat
    17221 ttaatatcgt aatggtattg atcatctgga gcggggcagt gcaaatcgat gcaggcgagc
    17281 ttcgtgtagg agatttgatt gcgtttatcg agtatatatt ccatgccttg ttctcattca
    17341 tgttatttgc tagtgtgttc atgatgtatc caagagcagc tgtttcagct ggacgtatcc
    17401 aagaagccct agatgccaaa ccttcgattg tagaagatcc aaatggtata tctgatacaa
    17461 aaactaaagg gtatatcgaa tttcaaaatg taacatttgc ttatccaggc cattcgcaag
    17521 agccagtgat acgaaatgtc agttttacag cctctccagg agagaccgtt gcttttattg
    17581 gaagtaccgg tagcggaaaa tctacgttga tccaattgat ccctcgcttt tatgatgtga
    17641 gtgaaggaaa gatcttgatc gatggagtag acgtgagaga ttatcggcta agcaagttgc
    17701 gagataagat tgggtttatt ccgcaaaaag cgctgctatt tactggaacg attgcagata
    17761 atttgcgata cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca gctgagattg
    17821 ctcaagcttc agaattcatt tctcgaaaac cagatggatt taatgaacat ttatcagaag
    17881 gtggttccaa tttttcaggt ggacaaaaac aacgattggc aatagccaga gccatcatac
    17941 gccgtccgga aatctatatt tttgatgaca gcttttctgc attagattat caaacggatg
    18001 cgaagttacg tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg atcgtagcac
    18061 aacgtgtggg aacgatcatg catgcagata agatcatcgt attaaatgaa ggagaagtcg
    18121 tcggtatggg gacgcatcgg gagttgttgg aaaaatgccc gatttattat gatattgccg
    18181 cttctcaatt atcaaaggag gaattagcat gaaaaaaacc ttttcttcct taaaacgttt
    18241 agctcgatat atcagaccgt atcgtgagac ttttctttta gtgatcctat ttacagttct
    18301 gacagtggct ttcaacgtgg ctttgccata tgttacagga cttcctacta cagaaatcag
    18361 ccgtaatatt gccaatggcg aatcaatcaa tttttcttat atattcaaat gtctcatttg
    18421 gatcacagtt gttggaatag ggtattgtat ttcgcaatta ctttctggtg ttttgatgac
    18481 aaatgtcgtt caaagtgcca tgcatgattt gagaagagac atagaagaaa aaatcaatcg
    18541 tcttccggtt tcatatttcg ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa
    18601 tgatgtagat gcagtcagcg gtgctttaca acaagctttt attggtatcg taaatgcgat
    18661 attgggaatt acgttggctg cagcaatgat gttttatatc cagccgatga tggcgctgat
    18721 ctctatgatc atgattccaa catcgatctg gatctcaaaa cgagtgatca atgcttcgca
    18781 gaaatatttt caaagcatgc agaattctct tggtgagcta aacggctacg tccaagaaaa
    18841 tatgacaggt tttagtgtat tgaaagtata tggacgcgaa aaagaaacat tggaaggatt
    18901 caaaaaagtt aatcacagct tgaaatactt tgggtttcgt gcagcattca tttctggctt
    18961 gatgatgcct ctagttcaat taactgccta tgctacttat attggtatgg ctgttctagg
    19021 aagcttttat gttattactg gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata
    19081 tatttggcaa atcagtcagc caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag
    19141 tgcatcagcg gcaacgaaac gtgtattcga gattttagat gagccagaag aaaaagaaaa
    19201 caaagtagat gcttcactgc cagaaacgat tttaggagat gtgacattcg atcatgtcga
    19261 ttttgcatat gatccgaaga aaccattaat ccaagatttg aattttgaag tgaaagctgg
    19321 tcaaaccgta gctgtggtcg gtccgaccgg tgccggcaag acgacattga tcaatctatt
    19381 gatgcgtttt tatgacgtag acaaaggagc aataaggatt gatggaatcg acaccaaatc
    19441 aatgagtcgc agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg catggcttta
    19501 tcaaggaacg attgcagata atatccgctt cggaaagctt gatgcgacag attatgaagt
    19561 agttgatgct gcgaaaacag caaacgtaga tcattttatc cgaacgatgc cagatggata
    19621 tgatatggcc atcaattctg agggagacaa tgtgtcttta ggacaaaaac agttgctgac
    19681 gattgcccga gcagtggtct ccgatcctaa aatcttgatt ttagatgaag caacaagttc
    19741 agtagacacc cgtttagaag cgttgattca aaaagcaatg gatcgcgtga tggagggacg
    19801 gacaagtttc gtcatcgctc accgtttatc gacaatccgt gatgcagatc tgattctagt
    19861 gatggatcaa ggtcagatca ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg
    19921 attttatgaa aaactgtata acagccaatt tgctgaggaa gcagaataag tcatataaga
    19981 ataaaacagt ttaaaactta ttttttgttc tctattcttg ataatggtaa agtaaagtta
    20041 tcaagaaaag tgaggacacg gagatggaac agtttttaaa tgaaaaaatt actgaagcca
    20101 aaagagaagt tgatcgttta acgcacgtct gtcagacgaa gccttttgat tcagctgatt
    20161 tttttgaaaa tgagtttcaa atcgaacgct tggcagcaca aatagaagtg tacgaattaa
    20221 ttttaaaaaa agtaatagaa gaaaatatag atgtacaata aaaacaggta aaacgtcagt
    20281 taccagatac tgtaaataga catctggcct gagcaggttt tacctgtttt ttttcttatg
    20341 aaataaatat atgctatgat acctttattc aataggatta gtttctacaa aaaatgaaag
    20401 gagaaaaata aatatggaac ggtttatagc atcaaatgcc acagtgattg gtgatgtgac
    20461 attgtcagaa gatgtgacga tctggtatca agctgtttta cgcggggaca gtaattggat
    20521 aaaaatagga caaagaacga atatccaaga tggtacaatc atccatgtcg atcatgatgc
    20581 acctgtagat attgcggaga atgtgacggt aggacatcag tgcatgctac atggctgtac
    20641 aatcgaaaaa ggggcgctga tcgggatggg gaccacgata ttgaatcatg cggttattgg
    20701 tgagaatagt ttgattggtg ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa
    20761 tgtcttggct tttggtcgcc cagctaaagt gatccgtcct ttgacaaaag aagaaataca
    20821 aaaaaacaaa gaaaacatcc agcattatgt cgagataggt cggatgcatg cgcaaggaaa
    20881 attcaaggag caaagatagg cagtagcatc cataaaataa aaaaggatgg tcagtttgag
    20941 ttgaccatcc ttttttattt ggtttagata agagagctta aaaaagcttc ctttgaaaca
    21001 gattaattta atgaagaact gttttccatg acttcatcga tcaatccata tgcttttgct
    21061 tgttcagctg tcatatagtt gtcacgatct gtatcttttt caatcacttc aagcggttgt
    21121 ccagtgcgtt cagctaaaat tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc
    21181 gcaatttcga tttcagttgc ttgcccttga gcgccgccaa gaggttgatg gatcatgatc
    21241 tcagcatttg gtaaagcaaa tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc
    21301 attgaagcag ccatacccat aacaattgtt tggacatctg ctttgacaaa gttcattgtg
    21361 tcgtaaatag ccatacctgc tgtaactgaa ccaccaggag aattgatata aagatagata
    21421 tctttttcag aatcttgagc gtctaagaaa agcaattggg cgatgatcga attagcaaga
    21481 tcatctgtta cttggccgct taacatgatg atacggtctt tcaataaacg tgagtagata
    21541 tcgtaagctc tttcacctcg tgaagattgt tcaatgactg taggaattaa attcataata
    21601 gagttcctcc ttgatttcgt ttagaaatag tgtaccataa tttagtttct aatacctaaa
    21661 atgtagtata catttttggt caaaaaaggt caaataaaaa acttaggcaa attagtcgca
    21721 taaacccgct ttatggtatg ataagcagag aaacaaaaca aggaggaaca acagattgat
    21781 agttggtatt actggcctta tcggagtgct gacaggtgct gtagttgtct atatcggcat
    21841 gatactgcaa atgcgtcact tggaaaataa agaacagcgt agacgtgaat tggaattgaa
    21901 agtcaaagaa atcgagacgc tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa
    21961 acggacaacg ctgatggaag aatatgtcag ctttgatgca tttgatgatt gttatatcac
    22021 gattgacgat tttgcgtatc tccaatcatt ttcagcgcaa aatagttttt atctgccaaa
    22081 ttatatttta gacgagctat ttaagaatat ctcccatcga aaagtgattt tatctccaga
    22141 tgaaacaatg aagattggtg gatatgctta taaaggcgga cgcgtgattt tagagaattt
    22201 tacggatagt ctgactgaaa tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg
    22261 ccagccgatt ggtttcttct cgaaagatga tgtttggagc tgatattttg aagcattgcc
    22321 gttgcacatg agagcgtggt aatgctttct ttttttctta atgatacaat agaactgaac
    22381 ggagtgaaga aagatgttac aaatctatgc ccatcgcgga agcagcggca cccacccaga
    22441 aaatacgctt cctgcatttg cagaagctgt acgagtaggt gcagatggta tcgaactaga
    22501 tgtccatcta agcaaagatg gttatttgat cgttatgcac gacgaagaag tggaccggac
    22561 gactaacggt aaagggctga ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc
    22621 agggggcaaa ttcagcaaag aatttccagc agctaaagtt cctgaattga gagaagtgtt
    22681 aggactgctc ctccaaaaaa attaccgagg gatgctaatg attgaaatta aaactgatca
    22741 gtatgaatat cccggtatcg aagaaaaatt agccgaaaca ttaaaaagta gagattggcc
    22801 ttttcaacat gcatattgca gctttcattt ggattctttg gaaaaaatcg cattactgga
    22861 accggagata cagctggact tattgatgag tacttctgat aaaaagatcc aactagcagc
    22921 agaaacccct tatatagaag gagtacatcc aaaaaatact tggttgttcg aacatccagt
    22981 ccaggcagct gattttccaa aagcaattcg tgcatggacg attaacgatg aagaagaaat
    23041 ggccaaatgt caagaactca aaatagatgg aataataact gattttccgg agaaagctct
    23101 tcggtggaaa caatcaacga aaagtaggta aaatatggaa aaagtattag tgatcgtcgg
    23161 tccgacagca gtcggtaaaa cagcgctaag tgtggaactc gcacaactat ttggaggaga
    23221 gatcatcagt ggtgattcat tgcaaatcta taaaaatctg gatatcggta cagcaaaaat
    23281 caaagaagaa gagatgaata atgttccgca tcatttgatt gatgtgatcg aacctactca
    23341 acgttactca gccgctgatt ttcagcattc aggtcgacaa ttgattaccg agattgcgaa
    23401 ccgagggcac ctacctatca tagctggtgg tacgggtctc tatatccaat cccttctata
    23461 tgattatcag ttaggagcga cagaagaagc gaatcctgag attagaaaaa agtatgaaac
    23521 gttagctgaa acaattggtt cccaagcatt atggaatcag ttgaaagaaa aagacccggc
    23581 agctgctgag aaaatccact ggaacaatca acgcaaagtg ataagagctc tggaagtttt
    23641 agagacgaca ggacatagta tcacggcacc taaagaagag ccaaagcaat tgtatgatta
    23701 cctcatgatt ggattagata cgaagcgtag tattctttat gaacgaatca atcaaagagt
    23761 cgatttgatg ctagcagaag gtttagtagc agaagcgcgt aaggtgtatg agcttggaga
    23821 agtccaggcc agtcaaggta ttggatataa agaatttttc ccttattttg aagggaaaga
    23881 aacattggaa gaagcaacag aacaagtcaa actacattcg agaagatacg cgaaacgtca
    23941 gttgacgtgg tttcgtaacc gtttggatgc acagtggttt gatcttataa gtgagccaaa
    24001 tcaaaaaagt cagattgagc aaacaatcaa aaaatggctg gaggcggaat aaaatggaag
    24061 aaaaagtgat catcgtaggg gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa
    24121 tggaagagct agttcagctg acgaataccg catcaggaga agtcgtattc acaattacac
    24181 aaaagagacc acaagtggat cggcaaacga ttatcggtaa agggaaatta caagaattag
    24241 ttcaacaagc ggatgcgcat gaagcggatt tgatcatctt taatcatgag ctaactccga
    24301 gacagagcca actgattacc gatgctgttg gtcttccggt aattgatcgc gtgcagctta
    24361 tcttagatat ttttgcgatg cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg
    24421 ctcagttaga atacctgctg cctcgtttag tagggcaagg gaaaacatta tctcgtcttg
    24481 gcggtgggat cggaacaaga gggccaggtg aaacaaagtt agaaacagac cgccgacata
    24541 tacggaataa aatttttgct gttaagaaag aattgaaaga agtagaagct catcgagagc
    24601 gcaatcgcca aaaaagaaaa aatagcgaga tttttcagat tggacttatt ggctacacga
    24661 atgccgggaa gtcaactatc ttgaatctat tgacacaagc ggatacttat tcgaaagatc
    24721 aattgtttgc aacgttagat ccattgacta agagatggcg ttttgctgaa ggatttgaga
    24781 ttactgtaac ggataccgtc gggttcatcc aagacctgcc tacgcaactg atcgatgctt
    24841 tccattctac attagaagaa agtcaaaata tggatctttt gctacatgta gtcgatgcta
    24901 gttctccaga tcgtatttta caggagcaga ctgtcttaaa attgatggat gaactgaata
    24961 tgaaagaaat gccgatactg actgtttaca ataaggcaga ccagatcgac ccagcgatgt
    25021 tcacgcctac gctattccca aatgtattga tttctactca gacgaaagaa ggtaagatgg
    25081 cgttgatcga agcaatcaaa cgtcagctga tggaactaat gaccccttat acactttctg
    25141 tgccaagcaa cgaaggacag aaattaagcg agttgagaag acaaacaatg gttttggacg
    25201 aagcgtacat ggaagaaaca aacgaatatg aagttaaagg gtttgcaaaa accaattcaa
    25261 aatggttaag aaaaaaccaa taaaatttta tttttttaa
//
LOCUS       DAZKSR010000037        25264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000037 DAZKSR010000000
VERSION     DAZKSR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.037"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..565)
                     /gene="dtd"
                     /locus_tag="ACG8XG_001351"
     CDS             complement(119..565)
                     /gene="dtd"
                     /locus_tag="ACG8XG_001351"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0773276.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(576..2789)
                     /locus_tag="ACG8XG_001352"
     CDS             complement(576..2789)
                     /locus_tag="ACG8XG_001352"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0773277.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(3022..3786)
                     /locus_tag="ACG8XG_001353"
     CDS             complement(3022..3786)
                     /locus_tag="ACG8XG_001353"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0773278.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="ACG8XG_001354"
     CDS             complement(3787..4734)
                     /gene="prmA"
                     /locus_tag="ACG8XG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0773279.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4747..5235)
                     /locus_tag="ACG8XG_001355"
     CDS             complement(4747..5235)
                     /locus_tag="ACG8XG_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0773280.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5201..5872)
                     /locus_tag="ACG8XG_001356"
     CDS             complement(5201..5872)
                     /locus_tag="ACG8XG_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0773281.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6010..7296
                     /locus_tag="ACG8XG_001357"
     CDS             6010..7296
                     /locus_tag="ACG8XG_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0773282.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7368..7562
                     /gene="ssrS"
                     /locus_tag="ACG8XG_001358"
     ncRNA           7368..7562
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8XG_001358"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7640..7924
                     /locus_tag="ACG8XG_001359"
     CDS             7640..7924
                     /locus_tag="ACG8XG_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773283.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7997..8476
                     /locus_tag="ACG8XG_001360"
     CDS             7997..8476
                     /locus_tag="ACG8XG_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0773284.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8699..8842
                     /locus_tag="ACG8XG_001361"
     CDS             8699..8842
                     /locus_tag="ACG8XG_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0773285.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9187..9315
                     /locus_tag="ACG8XG_001362"
     CDS             9187..9315
                     /locus_tag="ACG8XG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773286.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            9420..11003
                     /gene="abc-f"
                     /locus_tag="ACG8XG_001363"
     CDS             9420..11003
                     /gene="abc-f"
                     /locus_tag="ACG8XG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0773287.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11440..12624)
                     /locus_tag="ACG8XG_001364"
     CDS             complement(11440..12624)
                     /locus_tag="ACG8XG_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0773288.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12649..13656)
                     /locus_tag="ACG8XG_001365"
     CDS             complement(12649..13656)
                     /locus_tag="ACG8XG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0773289.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13828..14187)
                     /gene="comGG"
                     /locus_tag="ACG8XG_001366"
     CDS             complement(13828..14187)
                     /gene="comGG"
                     /locus_tag="ACG8XG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0773290.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14159..14584)
                     /gene="comGF"
                     /locus_tag="ACG8XG_001367"
     CDS             complement(14159..14584)
                     /gene="comGF"
                     /locus_tag="ACG8XG_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0773291.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14547..14894)
                     /locus_tag="ACG8XG_001368"
     CDS             complement(14547..14894)
                     /locus_tag="ACG8XG_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773292.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(14866..15336)
                     /gene="comGD"
                     /locus_tag="ACG8XG_001369"
     CDS             complement(14866..15336)
                     /gene="comGD"
                     /locus_tag="ACG8XG_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0773293.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15311..15589)
                     /gene="comGC"
                     /locus_tag="ACG8XG_001370"
     CDS             complement(15311..15589)
                     /gene="comGC"
                     /locus_tag="ACG8XG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0773294.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15586..16635)
                     /gene="comGB"
                     /locus_tag="ACG8XG_001371"
     CDS             complement(15586..16635)
                     /gene="comGB"
                     /locus_tag="ACG8XG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0773295.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16592..17521)
                     /gene="comGA"
                     /locus_tag="ACG8XG_001372"
     CDS             complement(16592..17521)
                     /gene="comGA"
                     /locus_tag="ACG8XG_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0773296.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17653..19407
                     /gene="ade"
                     /locus_tag="ACG8XG_001373"
     CDS             17653..19407
                     /gene="ade"
                     /locus_tag="ACG8XG_001373"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0773297.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="ACG8XG_001374"
     CDS             complement(19505..20167)
                     /gene="trmB"
                     /locus_tag="ACG8XG_001374"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0773298.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20237..21019)
                     /locus_tag="ACG8XG_001375"
     CDS             complement(20237..21019)
                     /locus_tag="ACG8XG_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0773299.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21223..22434)
                     /locus_tag="ACG8XG_001376"
     CDS             complement(21223..22434)
                     /locus_tag="ACG8XG_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773300.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22427..23164)
                     /locus_tag="ACG8XG_001377"
     CDS             complement(22427..23164)
                     /locus_tag="ACG8XG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773301.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23302..23736
                     /locus_tag="ACG8XG_001378"
     CDS             23302..23736
                     /locus_tag="ACG8XG_001378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0773302.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23729..24073
                     /locus_tag="ACG8XG_001379"
     CDS             23729..24073
                     /locus_tag="ACG8XG_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0773303.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24253..25263
                     /locus_tag="ACG8XG_001380"
     CDS             24253..25263
                     /locus_tag="ACG8XG_001380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0773304.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         7642 a         5264 c         4504 g         7854 t
ORIGIN      
        1 ttccgactgc tgacaaaaga agcgcttttt gcagacaaaa aagccactta agttagagtt
       61 tatcagtcta acttaagcga cgtgtttcat agtttttact agtgaaaacc agattacttt
      121 atttgttttt agaatcaata atgatcgtta caggtccatc attgacgaga gaaaccgaca
      181 tatctgctcc gaattgtccg gtagcgactg tgatgccgcg acttctgatt ccttcattga
      241 atgattcgta taaaggaata gctgtttctg gtcgtgcggc ttccacaaag cttggtcgat
      301 ttcctttttt cgtatccgca aacagtgtga attgagagat acttaaaatt tgtcctccca
      361 catcttctac actaatattc atttttcctt gttcatcttc aaaaatacgc atttgtgcaa
      421 ttttttttac cagatagtcc acatcttttt gcgtatcggt ttcatgaatg cctaataaaa
      481 caagtaggcc gtagccaatt tttccaattt cttgctgatc gactgacaca gcagctttac
      541 ttacccgttg aacaactgct ctcatatctg tcctcctatc cattcgtccg gcgtacactg
      601 tagacatcag gtactgcttt gattttatcc acgatactct ttaggtggga taaattctgg
      661 ataccaaccg tcaaatggat cgtcgccatt ttatctttat tcgtccgtgc ttcaacgctg
      721 ttgagatttt tcgttagcgc attgactgtt tgaagtacat cattcaataa gcctgaacga
      781 ttatagccgt aaatttccaa atctgcatca tactcttttc gtgtattcga tgtatcttcc
      841 cattcgactt caatcaaacg ttctgctaca tttggtttgt caggctgaac attcggacaa
      901 tctcgacgat gaatggatat ccctctgcct ttagtgatat aaccaacgat atcatcacca
      961 ggaatcggat tacagcagcg actgatacga attagtaagt tttctacccc ttgaatgaca
     1021 acgccacctt catgacgtac tttcattttg tcaggttctt ttttcggctg attcatgatt
     1081 tcttctgctt cttgcttttg ctgctcgatt ttttgttctt tacgttcttt ttcagtcaga
     1141 cgattggcca tcgtcaaggg gctaacttct ccatacccta cagctgcata gagatcatct
     1201 tctgtttgat aattaaaacg atcgagtgct tcttgcagtt tgttcttcgt caaaatatct
     1261 ttaggcgtaa atcctagatc tgtaatacat ttgaccacgg attcgtggcc tttgatcaca
     1321 ttttcttctc gatcttgggc tttgaagaaa cgtttgatct tatttcttgc tttgctagta
     1381 gcaactaatt tcaaccagtc gcgacttggg ccaaatgaat ttggagaagt catgatctca
     1441 atgatatctc cgtttttcaa tttgtaatca agctgcacca ttttgccatt tacttttgca
     1501 ccagtggttt tgttaccgat atctgtatgg atactgtatg caaagtccaa tggtccggat
     1561 ccttttggta actcagtaac gtctcctttt ggtgtgaaga cgtagacttt atcactaaag
     1621 atatctcctt ttacgccttc cataaattca gaagcatcat agctttcgtc ttgaagttcg
     1681 agtatctcat ggaaccagct taattgtttc gtcataccat ctggttctac tttttcattt
     1741 tttccttctt tataggccca gtgtgcagca accccgaatt cagcgatttc atgcatttcc
     1801 tgtgttcgga tttgaatttc taccggattt cctgccggac cgatcacggt agtatgcaaa
     1861 gattggtaca tattagcttt tggcatggca atataatcct taaatctgcc gggcattggt
     1921 ttccattttg tgtggattgc tcctaataca gcataacaat ccttgattga atctacgatc
     1981 actcgaatag ctagcaagtc ataaatttcg ttgaactgct ttttctgatc tttcatttta
     2041 cgatagattg aataaatgtg ttttggccgt ccatagattt ctgcaaaaat ccccagctct
     2101 tctgtcgcta ttcgaatatc ttcaacagta ccacttacgt atttttcacg ttcttctctc
     2161 ttcgtctgca tcaagtggac gatacggtaa tactgttttg gatttagata acgaagtgct
     2221 gtatcttcta attcccattt gatccggctg atccctagac gatgtgcaag aggcgcatag
     2281 atttctaaag tttcctgagc gatccttctt tgcttatctt ctcgcaggtg cttcaacgta
     2341 cgcatgttat gtagtctatc agctaatttg accatgataa ctcgtaaatc ttgagccatt
     2401 gctagcagca tctttcggtg gttttctgct agctgctctt cgtgggattt atattttatt
     2461 ttccctaatt tggttacgcc gtccactaac atagcaacgt catcgccaaa ttcttctctc
     2521 agatcttcta aagtgatttc tgtatcttca acaacatcat gtaagaagcc tgtagccact
     2581 gtatggggat ccatttttaa atctgctaat atacctgcga cttggatagg atgaataatg
     2641 tagggttcgc ctgattttct aaactgtccg tcatgtgcag cagtcgcgta ttcgcatgct
     2701 ttctcaacaa atgctacatg ttcgggaccc atgtattgac taacgagctt tatgacaccg
     2761 ggtcccgtta aaatttcttc ttttgtcaca ggaatcgatc cccctttctt ttttcggtaa
     2821 aacgggaatc gggacaaata tctgtttgat aattgtccca aaatccgtgc agttatacgt
     2881 ttttttacgg aaatgttcgc tttttaaaag ctttttcaaa cgatttttcg tatccattgt
     2941 ataaataatt gtttctatta tacaaattgt tgactatttt ttcaattctt tctgtcgata
     3001 ctcttttatt aatgaagaac gttagctgaa agaaggattc attaattccc attgatagct
     3061 agcagcactt aataaataaa gaggtgctgt ttctgtacga aggattcttg gtcctaatcc
     3121 acaaagtatc cctttgtgtt ctacaaaata atcgacttca tcgcgggata atccaccttc
     3181 tggtccaaag acagctaaga ttcgactatc tggttttgtt tcactaagaa ttttggctgc
     3241 atttgatttt tcgccttgct ttgctgattc ctcataagca accaagacca cgtcataatc
     3301 atctagccgc tcaaataaat cttgtttcgt ttccaataac tctactgatg gcacgatctg
     3361 ccgatgggat tgttccgctg cctcttgagc aatcttttca agccgttgtt gtcgtttcat
     3421 acgttttttt gcatcccatt taacgacaga ggctgatgcc gggaaaccaa gaaaagcttg
     3481 cgctcctaat tcagtggatt tctgaacgat ccaatccagc ttttcccctt taggatagcc
     3541 actcgctatg gtaatcgaac ttggcagttc cttttgattt tcttcttttt taatttcttt
     3601 cagataaaca gcctcttcag taatctccgt gatttctgca acaatggcta tttgatcatt
     3661 gaatgccaaa aaaacttcat cctcgggttc catcctcata acccgtacaa tgtggtgata
     3721 gttttcccct tctaaaacaa aatagtcttt tgattcatat gattctttta aaaaataccg
     3781 ctgcatctat tccacctctg gttttttcaa aataattgca taccagtctt tttgctggaa
     3841 aatttgatcc acgataaacc cttgttctaa catggcatcc agaaccattt gcttcttctc
     3901 ttcaatgatt cctgaaacga tcagcgtgcc tgtttgcttc agtaaacgcc aagcatctgg
     3961 gatcatcaat aagatgatat ctgctaaaat gttcgcaacg attacatcac tttcaatctc
     4021 gattcctgtc aaaagatcgt tagctgatac atggacatct ttggctattt cattcatatc
     4081 catattttct ttcgccgaac gaacggctac ttcgtctaaa tcaaaggcat agacatcttt
     4141 tgcaccaaga tattttgctg caatacttag cacgcccgac ccagttccca catctagcaa
     4201 tgtttcacca ccacgcaaga ctgtttctag tgctgtcaat gtcaaggaag tcgtgggatg
     4261 agtgcctgtg ccaaatgcca taccaggatc tagtgtgata attttttcta gaggatcttc
     4321 tgtctggtag ttttcccagc ttggcgcaac cgtcagcatt cgcgtaatac gcaccggatg
     4381 atagtatttc ttccaggctg ttgcccagtt gctttcctct acttcactaa ctgttacttt
     4441 attttttcca atagctagac caaactcagg taattgtgtg atacgatctt tgataaaagg
     4501 caagatttcc ggtaagaaag tcgtttccgg aaaataagcc atgactagtg cgccttcttt
     4561 cagcgatgta aaatcatttt tatctaaaat ctcaccatat tgatccgact gaaaatgttc
     4621 aacatctaaa gaatcttcaa tcgcgacacc actcgcccct gcctccatca aaatattagc
     4681 aactgcttcg actgcttcac tttcagtttc tacttttact tcattccatt tcatatctgt
     4741 cactccttaa taacttgggt acggtaaata agtatctgct tcattttgct gttttacttc
     4801 tactaattct gctagttgct gattatccca gtgaagctcg aatacagctt cgtcatcgtc
     4861 ttcatccaaa aaatcaagca aatcgctttg cccttgagct aatacttcat ttagatattc
     4921 cactaaagaa tcagctactg attttgccaa cccttttttt ccttcataag ggattactgc
     4981 tagataattg cgttcatcaa aaacagattt ctgaggatta aaaagaagta cgccgtcttc
     5041 aaattcgata attggttctt gcgaaacgat cccttctgca tcatcaatct caaattgtgc
     5101 tttattttct gcaaacaagc gaacaacgat ctcgatcgta tgattttttg tatcccaatc
     5161 taaagctaca tcatagtctg tcacttgttt ttcaattatt tcatctaaat atgttaatag
     5221 atccttcttt ttcattttct ttcctccaac cccaatcttt ctcgatttgg ttttttcttt
     5281 gtaaagagat ataaggattt cccgacacaa caaaacgcaa cggcttttct gtccatacac
     5341 ctttgtttgg gataccgatt ctaggcaaag cattgatctt ttttggtgtc ttttttttct
     5401 caaaaactaa gcgtaacgaa ctatctgcta tagactgacc ataaagttct tgaggaattg
     5461 ctaatgcttc cactaatttt cccggaccgt tgctgatatc tggaccagtt tttccgccac
     5521 gatttttact catttgatca atcattgcgg cgggttcgat tgcacgtatc attactcctt
     5581 gaggaatccc ttctggctga gtaatgatgt tcaaaatccg atgagtatgc atcgtgtaaa
     5641 ggtagatcgt acctggtttt tcatacattg cccgcacacg cggtgttctt cttaaaccat
     5701 agctatgggc ggcctcgtct tctggaccta ggtatgcttc cgcgtctaca atatatccag
     5761 ctaatcttcc ttctggtgtg tcatgctcca aatacatccc caataaatcc cgtgccactt
     5821 ctggcgtcgt tttattccaa aaaaattcat ctatcttttg tttatccgtc atttttctca
     5881 cctactttat catacacaat attagttgga taagctatga aaacaaaaca acagaaggct
     5941 gttctttcta ggcatcttgc taaatatgat ataattaagg gacgtaaaaa caagaaggag
     6001 gaaaatccta tgcaacagcc attggcttat cgcatgagac ctcgaacgat cgatgaagtc
     6061 gttggtcagc agcatctagt cggggaagga aaaatcatac gccgcatggt cgatgctcgc
     6121 atgctctctt ccatgattct atatggacct cctggtactg gaaaaacgag tatcgcaagt
     6181 gcgatcgccg gctctactaa ttatgcattt cgcatgctaa acgccgctac agattcaaaa
     6241 aaggatctac aagttgtagc ggaagaagca aaaatgagtg gtacagtgat cctcttattg
     6301 gatgaagtac accgattaga taaaacaaaa caggattttc tgcttcctca tttagaaagt
     6361 ggccgaatca tcttgatcgg agcgactact gagaatcctt atattaccat caatccagcg
     6421 atccgcagcc gtacacagat ctttgaagtc aaaccattaa cagaacaaga catccagttg
     6481 gcagtcgaac atgctttaag ggataaagaa cgcggccttg gacaacaagc aatccagtta
     6541 gacgaagatg ctcttcttca cctttcgcgt gcaacgaacg gcgatttgcg gagtgcattg
     6601 aatgggctgg aactggctac actatcgact ccttcagata aagaaggaag gattcatctt
     6661 acactgtcga tcattgaaga atgtgtccaa agaaaagctc ttacccatga caaaaatgga
     6721 gatgctcatt atgacgttat ttcggctttt caaaaatcga ttcgtggaag tgatgtggat
     6781 gctgcacttc attatctcgc ccgtttagtt gaggcaggtg atctggcaag tatttgccgg
     6841 cgtctgatgg ttatcggtta tgaagatata ggtttaggta atcctgctgc tgcggctcgt
     6901 acagtgaacg ctgtattagc tgctgaaaaa ttgggattgc ctgaggcacg tatcccacta
     6961 gcagacgtcg tcgtcgatct ttgcttgtcg ccaaaatcca attctgccta catggcatta
     7021 gatgcagcac ttgctgatat tcgagagggc aaagctggag atgttcctga tcatttgaga
     7081 gatagccatt acaaaggtgc gaaatcactc aaccgtggcg tgggttatca gtatcctcat
     7141 cattttgatc aggcttgggt caatcagcaa tatttaccag ataaactgaa gaatgctcag
     7201 tattatcagc caaaagatac agggaaatat gaacaggctc tgggtcaaca gtattaccgt
     7261 ataaaagagt ggaaaaagca tcctccaaaa aactagacaa gaaaacggtt ctgtttctgc
     7321 gagctctctc attgctcttt ttaaattcat atgctactat agaggtggga aatctgtgat
     7381 gttcgaattg tcctctttgt gtgttgaccg aacattttta ttgatatctt gggacccgtc
     7441 ttgtgtcact gctggtaaca tgccttatca tttggtagtt cgaagctacg accatgggac
     7501 ccacctgctt gaattcgcgg gatcaatact acaggacaaa caacggcatt aacggagatt
     7561 accatctttt aaagaataaa gcaatttgct cttggcaaat tgctttttct atttgttgct
     7621 tattttggag gatttgttca tgattaggat tttaatgatt atcgccactt tagtattatt
     7681 attcgttagc tattatttat ttaataaaca ggatatcttt tttgtattga tcaacaaaaa
     7741 tgataaaaat caaggattcc tacaatttta cggagctgca tatgctgttt taggcgtaat
     7801 gggtatttta acagcttttt tcaatcaacg ctttattgcc ttggtctttt tactgatcgt
     7861 aattattgtt tctgccacct tcagtatccg atttgcaaaa aaaattgctg aaccaaagca
     7921 gtaaataatt ctctttttcc ctaaagtgtt ctacaataaa agagtacaat acgattgact
     7981 tgaggagtga atgcttatgt tacaacaata caaaaaaatc atggttgccg tagatggttc
     8041 tgatgaagct gaattagctt ttaaaaaggc ggtcaatgta gctatccgaa ataacggaga
     8101 acttctatta gcacatgtca ttgacacacg ttctttccaa accgtttcat cctttgatgg
     8161 aatgcttgca gaacaagcta ctgaaatggc aaagcaaaca ttggcagatt atgaaagcaa
     8221 tgcgaagaaa gctggcttga ataatgtaac ctctgtcgta gaatatggct caccaaaaca
     8281 aattattgcc agagaaatcc cagaagataa tcaagtagat ttgattatgt taggagccac
     8341 aggattgaat gctgtagaac gtttatttat tggttctgtt tctgaatatg ttattagaaa
     8401 cgcagcttgc gatgtgcttg ttgtccgaac tgatttagaa aaccaattgc cagctaaaga
     8461 ccacaccaaa gcttaacacg tagacgacct gccaactatc ggcaggtttt tttgcacaaa
     8521 aaaccgtgat aaaaacaaaa gacgcggcaa gcactcatcg gtctttataa tgtttaaaat
     8581 aaagggaaaa cagtcaatga gttcaagtaa aataaaaaaa agtgaacacc agggcgatta
     8641 gtcgccctgg tgtggtttaa aacgacctaa gcatgccaaa gcttattggt tcgtcaatgt
     8701 ggaagtttcc gatgaatctg atacgaaaaa atatgaagta tcgatggcgg ataccgattt
     8761 tatcgccaaa ctaaaatttt atttcgggtt cgggtaccgc gagaaggcaa gtgctacatt
     8821 cggtaaatca ttcaagaatt gatgaaaggc taccgcttgc cattgcaacc acaacgatac
     8881 tcctgaacgc gatgttggtg agatttccaa agagaagagc tgtttaatgc aacggtatta
     8941 agcaaatggc aaaaggaagt catgtagaca aagtttttta tcaaacacca tatgatttcg
     9001 cctgaacgct ggtttagctc agaatacaat gatgtggtcc acgagacatt accctcagtc
     9061 agataaaaaa agctctctga cagaattaca catcgataaa tattaagtaa atgtcagcgc
     9121 tagttgaact ttctgacact ttcaggtaca ctggaaaaga caactacacg aaggagggaa
     9181 aatactatga tgcgcatgac taaaataaca agtaatgaaa attaccaacg ataccatatc
     9241 gcatttagcc atgcaattgg ttttacagtt gctgttgacg ggacaagttt gtccaaaaca
     9301 gttgaattca cgtaaaatca atggccatct caaggtattt ttctcatgat aatctgagtt
     9361 taaataacac aggccatgga taaccatggc ctttttgagt ggtctcaagg agagagatca
     9421 tgatcataca agcaatttct attaaaaaaa caatcggcgg gaatccatta tttgaggatt
     9481 tatcctgtca aattgaagct ggtgaaaaaa ttgtcttagt cggtatcaac ggaactggca
     9541 aatcaacctt tttacaaatc ttgaccggca gagaaggtgt cgacagcggt gttatcagtc
     9601 gcaaaaaagg cttaaagatc gggacggtcg aacaagaact tacagtttcc gaagcgaccg
     9661 tcaaccacta cttgctccat tctgccgctg agatacagga cctcaaacag caaatgagtc
     9721 gctacgaagc attgatgaca gaaccagata caaacttaga gaagtgtctg gcccgttacg
     9781 gtgagctgca gcatcgcttt gaagaactag gcggttacgt attagaagac cggattgcag
     9841 cgatcttgca aggcttaggg attccacacc gtcaggaagc acggttaaca gaacttagcg
     9901 gaggcgaacg agttaaagtc gctttagcca aaattttagc agccgatgcc gatttacttt
     9961 tactcgatga accgaccaat catttagatc ttaacagtat tcgctggttg gaaaactatt
    10021 tacagaatac aaaaaaatcg gttctggtcg tttctcatga tcggcaattt ctcgatcaag
    10081 tcaccacaaa aacatgggaa ctggaagaag gtgcattgat tgagtatccc ggaaactaca
    10141 gccgttttcg agtcttgaaa gaagctcgct tggctgagct gacaaaaaat tacgaactgc
    10201 agcaaaaaga agtccagcgg ttgaaagtca tgattcgtcg ttttcgtcag tgggcacatg
    10261 aaggagacaa tgagtccttc ttcaaaaaag ccaaagaact ggaacgccgc ctggcgaaac
    10321 tgacactcgt caaaccgcca cctcccccga aaaatcggct gcaaagcttg tctaatggtg
    10381 gaaaatctgg caaagaagtt ttcatcattc aaaacttgca ccagcagtac gctgatcaag
    10441 tactattcaa agacagcagt tttgcagtct atcgtggaga tcatttagcc atcatcggcg
    10501 acaatggtgc aggaaaatca accttactca aattattact cggtgaggaa aagccttacg
    10561 gtggcaccat caaattaggt agtagcttgc aaatcggtta tttgccgcag cagctgcagt
    10621 ttgccgatcc cgatagccgt ttgctggctt acgccatcac attgactgga aacgaagaag
    10681 tcgctcgacg gcaattagcc aaaagtggct tttatcaaag cgatgtgggt aaacggatca
    10741 aagatttatc cggtggtgaa aaaatccgtt tggcgttgct gaaactcttt ttagaaaaaa
    10801 tcaacgtcct gattttggat gaaccaacca atcacctcga cagctatgct cgagaagaaa
    10861 ttgaagaaat gctgcaagac tatcaaggaa cgctcctagc agtcagccat gaccgctact
    10921 ttttgcagca acacttccaa gaagcactcg ttattgatca ggagcagatc agccgccagc
    10981 cgctcggcca actgattgac taggacgttt tttcgcaaca aaaaacttga ttttcatacc
    11041 tgtgctttca cgattcaggc aaagtacagg tggaaaatca agtttttatt attcgatcaa
    11101 tgaatgtttc ggatcggttt tctagctaaa cataaagcga agaataaagt cgacagccca
    11161 cgaggctgat ctaaaaatgt tttttctttt tacatatgta atgtccctta taaagatgaa
    11221 tgaaaataaa atattgggat tttcctgaaa atcaataaat aaaatgccag tcttttccct
    11281 tttgaccact tttccaagca aaattcccta agaagatcct gtaaaaaaga agggaaattg
    11341 aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa cacatgcaag aattccctct
    11401 tgcatgcgtt tcatctttta tcctaacgaa tgctttggct tagccgcgca gacgctccac
    11461 atcacgggca atcattagtt cctcatctgt tgggatcact aatactctta cttttgattc
    11521 ttttgtagag atatctactt cttcaccacg caattcattc aacgctggat cgatttcaca
    11581 accaaaccaa gacatacctt tgatgatttc actacgtaca tgtccgtcat tctcgccgat
    11641 accagcagta aagacaattg catcaacacc attcatcgtt gtcacataac cgccgatgta
    11701 ttttcggatg cggtccacga aaatgtcata agctaaacga atatgttctt tttcatagtt
    11761 cttttcaaga tcacgcatat cactagacaa tcctgttagg cctagcagac cagatttctt
    11821 gttcaaaaca ttgatcattt catcaatatc aatctctaat ttttccatta aatatgggag
    11881 aacagctgga tcgatatcac ctgaacgtgt acccattgtc accccagcta atggtgtgaa
    11941 tcccatagaa gtatccatgg atttacctcc atcgactgct gtgatcgata caccatttcc
    12001 taagtggcaa gtgatgattt ttagttcttc gatcggtttg ttcaataatt ttgccgcacg
    12061 ttccgacaca taacgatgac ttgtgccatg gaagccatat tttcttactt tgaaatcttc
    12121 ataataagac accggtaagc tatataaata attttgcttt ggcattgttg agtggaaaga
    12181 tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc tttttaaacg ctttgatccc
    12241 cattaagttt gcaggattat gcaaaggagc aaattctgct aattcagcaa tgcggtccat
    12301 tactttctca tcgatcacta ctgaatcccc ataagtttct cctccgtgaa caacacggtg
    12361 ccctactccg gtgatttcat cataagagct taaaatttgc agttcaatca actgatctaa
    12421 cagcattttt actgctactt cgtggttttc gatatccatg atttgttcga atttttgatt
    12481 atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg atacgttcga cgatcccttt
    12541 cgcaactacg gtttcttctg gcatttgata taattgccat ttcaaacttg aactcccagc
    12601 attgattgca attgttttag acatatgact ctccttctcg ttgaatgatt acagagcaga
    12661 tgatttccag tttttaaact cgttgaaaaa ttccgtcacc ttctctggtt cttttaatga
    12721 agcaagtttc acaagcaaga cttcttttac ttgttgtgct tgtggtcctt ttttctgtaa
    12781 gatcaaaata cttttttgcg attgtttatt acggaacaat tcatctggca attgaatcat
    12841 tccttgaaga taaccttctt ctttaaacca ttttttgatt tcttcacttt gatccgtttc
    12901 taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc ggcttcacat atttcattga
    12961 ttgctctagc aataaatgat gtgcataact atggccttct tcggtacttg ttaaaaactc
    13021 ttttgctttt tcgtcattcg gataataacc gattggcaaa tcgctgattg caaaatcgac
    13081 aggatctatt aataattcct gcaacccgtc ttgatgaaag tattgaacat ttgcttgtgt
    13141 caaatcactt gtagaagctg cgacagccaa caatgtatca tcaatatcca caccaattcc
    13201 ttctgtttgg taccccgcat tcgataggtt taaaagaact gttaataaca agttgcccat
    13261 tcctgccgca atatccagaa tcttaaccgg tgctttttta tttgtgtata gttgttcaat
    13321 aagaaaaaca aataaaaaac cgatactatc aggagtgagt tgatggttcg cctgcaaatg
    13381 ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt ctgcgccatt cttctggctc
    13441 gaaagaaagt ttcttcaact cttcataaag agctgtgatc cgttgcgttg tctcttttgt
    13501 cggcactcca tccaccacac gtacttgata atcatctatt aaattttctg cattttcgac
    13561 ataggcttct aaaaaggaag ttcccaatgc attctgtaaa agctgaatag cttccaggtt
    13621 ttgattaaac ccttgttcca ttttttctgg taacatggtg cacctcattt ctagatatat
    13681 tataacaatt ttttcacctg tttaattcta tcgaacaatt gacaaaattt caatcgtttt
    13741 cactgttttt tatttgtgaa acggcaaaca tttttttatt aaaaacagtg ttataacaga
    13801 aaagagggcg ttttcacgaa cattccatca ttctgtacta gtatctgtat cattttttac
    13861 ttcttctgtg aaccgaaaga taaacggtga gacttccacc tctaagaata gggactgttg
    13921 tacccactga tatgttacag atccagttgt gtagtaaaaa actccttccg aactttcggc
    13981 aagcgtagac tgttccgata aagccatctg cttcattatt tttgctatat aataattttt
    14041 gattcgtata ttaagatcca ttgacaaacg gtagctttct aatgctgcca tcaataaaaa
    14101 cgtacataaa agaaataaaa gcaagacaga aaataaaata gagcccttat aacattttct
    14161 actgatcgtt ttttgtccat atggcatatc catcttctcc attttcaaag tgtacataaa
    14221 aagaaattgt gtgttctttt tccaataatt gaagctcagt gatacttgtt aacataggtt
    14281 gatgtccgcc ggaagttctt cttttacgta tcattgcctg atatctttcg atgatatact
    14341 gtttatcctc gatcgtgtag tgcagttgct gatttttcac agagacattg tgactgctct
    14401 tgagttcttc ttctaattgt gctaaaaata ttagccactc tttttgatct ttacgctgca
    14461 aaaattcact tacttggatg gtttgctgaa tcaaaagatt gaatgtgagc aatagactgc
    14521 tcaatatcat cagtccaatt agacattcaa tcagcgtaaa tgctggaatt acttgttttt
    14581 tcaatactga tcgcttcttt ctccatattg acaaacactt tttcgattac tccattatta
    14641 tcgacaattt ggatatcgta tgggatcatt tcttcccttc gctctggtct tttgtgatac
    14701 atccgctgcg tcttgatatc ttcataaatc attcgaataa atagcagttc ctccccactt
    14761 ttcttttctg cctgaagcaa aaacagctgg ccaaagctca ctaagtagac acagacagaa
    14821 gcgatcataa gcgctgtaag ggactccaac aaaacaaatc ctgttttatt caattctttt
    14881 tgtatagcga ccactcccta tctggaattg atacgtaatc gtccgctctt tttcttccca
    14941 ataaaattga taagctttta gtgaactttc attcccggtt ctagcagcaa aagtaataga
    15001 agcatggcgt ttcaaggtaa tctcatcagg aatatctaat acttcccaat ttattttatc
    15061 tggattcgga acatgaaaaa tgatttgatt ctcttcacta ttccaataaa agcctgtttg
    15121 gatctgattc acaatagcga ttttttgcgt agaatagatt cgtttttcaa attggccgaa
    15181 aaattgatag actgccaatt cattcttcca tgcagaaaat gcgagaactg gaaaagaaag
    15241 gatcgttgta agtaataaaa tcagtaagag tgattcaaac aacgtatatc ctgatgctaa
    15301 aaccttacct tcaaggccgc tgatagattt ctgcatgttc tcgtttgata tagccttcat
    15361 tcaacaattc ctccacagta ggaactcttc cattattttg aagcgcatag agctcttttt
    15421 gtgtgtctac caactggatg atcgcttcac gactttcttg gtctacatga ttcctataac
    15481 gcgacaaatt agggacgaac aacaacacta atacactgat gatcagtaag acgattagca
    15541 tttcgatcaa tgtaaaaccg ctataataag atatttttct ctttttcatg gcaaaacctc
    15601 ctctatgctt ccgtaaacag gtaataaaac agcggcataa actaataaaa tcaaagccgc
    15661 tacgcccaaa aaaatgattg gctgaataaa atgcatccag tgatgaagac gtttgaaaag
    15721 ctgttttctt gtccattcgc tgtaaaaaat cagttcttta cctaaacttc cctttgcctc
    15781 tccctgtaaa acaattttac tgaattcttc tgatagaaaa cgataattct ggaactgctc
    15841 agccaatgac atacctgcct ccaatccttg aatcaactta gaggcaagat gctgaatcag
    15901 actttcttgt ttcgtttcct ttaagcagac tatgatttgc cttaattcga aaccttcatg
    15961 aaaaagcttt ccaatctcta gtgacaaata agccgattga tacaaggaat atatcggtcc
    16021 aatcaataat tgtctgctta tccattcact ctgatcaaca ggagacaact tcatcagacg
    16081 ataccttact agtaaagcca atagagtaac cgctaggatg aatagtagaa cataccaatg
    16141 aaagacttgt atcagctgaa taccccaatg ctcttgcatg accattcctg aaccaataag
    16201 ctgaggcaaa acgaacaacc gcatactaaa caagatacct agcaaaaagg tgagcaatag
    16261 catcggatat gcaagcagct ttttcaaatc attgcgaaat ccttcaacaa gccgaaactg
    16321 tttggcaatt tccaacaaag ttgtcggcag attcccatga cgttcagcta actcgatctg
    16381 taccagttgc tctgctccat atcctatttg cgaaagtacc tcactaaacg cttgcccttc
    16441 ctttagctga tgttggatat ttgccaaaat aacaggtgga aacgaacgga tcgtgagtaa
    16501 taaataaagg gattcttgca gactaaaacc agataaaagc agttctccca tggtttgtgc
    16561 aaactggttt tgctggcttc ttgttaatcc tttagaatag ttctgactgc caaatatttt
    16621 cctgagcttc ttcataactt tcctcccatg atttttttac tgattcttcc tccataaatt
    16681 gataactcca taaaacagaa ggatggtgat tggcatcttc caaaagttct tgataaatga
    16741 caccagctaa acattcagat agctcttctt ttttctttgt cagttcatgg attctgacca
    16801 atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac ccggtgccct gttagtgctg
    16861 ctcttaccgc agcatgtgca gttgattcat ctcttatctc gccaatgatt agcaagtctg
    16921 gacgatgccg taatgcgagt ctcaaaagtg tatcataagt ctgtgctatt ttcggattga
    16981 tctgtagctg cagaaaaaac ggttcttcta tctctacagg atcttcgata gtaatcactt
    17041 gcaatttttc ttccaaagcc aaccgataca ttagagaagt cttgcctgat ccgactggtc
    17101 cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa aatagttgca aaatctcgag
    17161 aactaaagca acgatggggt gtttcaaaag taccgtataa tagccgaatc actaaactct
    17221 ctcgttgaag gtaatctcct acagaagaca accgcagacg aacttcctgt ttttctaaag
    17281 aataagtaaa tgcacctaac tgagcttttc ttttttctcc gatatccatc tgtcccaaat
    17341 acttaaatct tgagatcagt tgctgtccat ctgccgaaga gactttcttc cagaatgttc
    17401 gtttttttcc atagcgaaaa tagatgtaaa atacttctga agctggcaat atatatacgt
    17461 cttgggcatg tttggcaaaa ctttcactta tcatttgatc cgataatcgt tttatttcca
    17521 ttattctccc ctccacaaaa aacatacgtc tttttgatct ttttgtttca aataaaatga
    17581 atttacaaaa aaatattggc aaaaaccgaa tttatgttat acttataaaa atttatcttg
    17641 ggaggcactc cattggaaac aacattaagg aaatatatcg aagtagcaag taaaaaaaag
    17701 cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg tctttacgaa agaaatcatg
    17761 gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa tcggtgaata cgaaggaaaa
    17821 agcgtacata atgctaatgg gcaatatcta gtgccaggat tcatcgatgg ccatgtgcat
    17881 atcgaaagct cacttttatc accaagcgaa ttttcaaaag tatccttgct tcatggagtt
    17941 actactgtag tgacagatcc ccatgaaata ggaaacattg ctgggagtac aggtcttgag
    18001 tttatgatag aagatgcaag acaaacactc atgaatatct tttttatgct accttcttgt
    18061 gtacctgtaa ccccttttga gaccaatggt gcaaatttaa atgcaaaaaa attagcccct
    18121 tttttacaaa aacccgaagt tttaggctta gctgaagtca tgaactatca agcagtagca
    18181 aataatgaaa cagacatcct agaaaagatc caattgatgc accagcataa gaaaaaaatt
    18241 gacgggcatg cagctggtat cggcatggaa gagttaaatg tttatcctgc agcaggtatt
    18301 cggacggatc atgaagctac aactgcaaaa gaagctaaag aacgtttgga tcttggtatg
    18361 tatttaatgg ttcgagaagg gaccgttgca aaagatctgg catcgctact accggcgatc
    18421 acacctgaaa attcaagaag atgcttcttt gtcaccgatg ataaactgat caatgattta
    18481 gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta ttgcacttgg aatcgatcca
    18541 ttacaagcca ttcagatggc aacattgaat gcagcagaat gttttgattt gaaaacacta
    18601 ggtgcgattg cccctgggta tcaagctgat ttcttcttaa cagacgatct taccaagctg
    18661 ccaattcatg aagttttcta taagggaaca ttagttgttc aatctgggca actccaagac
    18721 catttatttg ctgatcattc aaattcatat acgtgttgtc tgccaaaact aaacgctcaa
    18781 ccattaaatc cacattcgtt agagctgctt ctttcttctt cacaagccca tgtgatcgaa
    18841 attatcccga atagtctagt gactaaagat ttggtagaaa aagtggaccg acaaggcgaa
    18901 cgattcattc cttcaatcga aaaagaccaa ttaaagattg cggtgatcga gcgtcaccat
    18961 caaacgggaa acattggatt aggcatagtg aaaggatttc agctgaaaca aggagcgata
    19021 gcgacaacga ttgcccatga ctcgcataat ctagttgtcg ttgggacaaa cgacgaggat
    19081 atgctgtatg ctgctaatct tttgattcaa aaaggtggcg ggatgaccgt tgtcaacaat
    19141 cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa tcatgacaca ggaacctttt
    19201 tcgatcgtct atgaacagtt gaatgaatta ataactaaag cacatcaact aggagcagct
    19261 aaaacctttg atcctttttt gacactttct ttcttaacat tatctgtcat acccgaattg
    19321 aagatcaccg acaaaggatt attttcattt tctcgcttcc aattgattcc accttgtgga
    19381 aaaaccgatg aaataaatac attttaaatc aaataaatcc cagaaaatac attacatctg
    19441 taatgttcat tttctgggat ttatttatgg atattaatgt ttttatgaaa gcagaaagtt
    19501 agtttcacag acttttcttt ttggtcttat cttgaaattg agcttctact cgataaatac
    19561 gttgaccttt tgaagaaaat ttttcttcat actccgtcat aatattgcct tcaaattgac
    19621 tttgatgtaa atctaaccat acctgtttga ggatcatgcc atattgagaa aaacttgcca
    19681 gagaatactc aaacaatcct tgattatctg ttttgaaatg gatttctcca tttggacgaa
    19741 gaatctgttc atctacagca agaaaagtct tatatgtcaa tcgccgtttt tcatgacgtt
    19801 tttttggcca tggatcggaa aaattcagat agatttgatc tacttcgctg tcagcgaaat
    19861 actctgtcaa cgctgatcca tccacatgta gaagtttcag gttaggaagc ggttgttcga
    19921 tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat ttctactcca atataattga
    19981 tttcaggatg ggcttttgcc attccagtga tgaactggcc tttccccata ccaatctcaa
    20041 tgtggattgg atgatcgttt tcaaacaatt cattccattt tcccttccac aatgttggat
    20101 cactaattac aaaattcggg tgagccgcta acatttccgc tgctcccgga cgatttcgga
    20161 cacgcattat ttatctccat tcctttcctc taattaaaaa ggtctggcca aatctccgca
    20221 gagtttgggc caaacctcag ccactgaaaa ttcgttttaa ttgtaaaatt tcttcgttca
    20281 tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc ttggagcaaa ttcatggccg
    20341 cataccaatg aaatttttca agcatttcgt cagtcgggcg tatgccataa ctgatcagcc
    20401 actgattcca gctagacagt ggcacataat ttcctaaaat cgttgcaata tccaccgcag
    20461 ggtctgcaaa catgattgaa tcccaatcga ctagatataa ataattttta catactaacc
    20521 aattacgatg gtttacatct ccgtgtacta ctgataagtt ttccttatct attgacggga
    20581 tctgctcacg aagaaaacga tggacaattt gcagaaattg atttttctgg agttcttccg
    20641 gtaattcttt ttcatatttt tgcagcatct tctccggcgt catgacacga ccaccgatac
    20701 gggtcagcat ggttttaaga gaatttgaat gatgtaaatg gtacaaaaca tcaatcacgt
    20761 cgtttctttg accaatctca ttaggagtca gcaagcgacc ttccagccac tcttgtgctg
    20821 tcaaagtatc gccattcccc gttcgttttg tccaaacaag ttttggtgta attccttccc
    20881 ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat aaagactttg tcctcatcac
    20941 gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg acgtaaccgc cactcttggt
    21001 ctaactgaaa ctccatcatg cattctcccg tcctcttact ctttgttttg cagtacttat
    21061 tattctagtc gcctgaacac tacacgtcaa gcacatatac gttctttttc atttaattgc
    21121 cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa cagcattctt actgtcaacg
    21181 ctctattttt cattctcatt tttaccctac acagctttgt tttcagtctg acatcttctt
    21241 caaacgagtt ggtacatata gtttaacaaa gagcatagta acaatcaaat aagccgctag
    21301 cggaacccat cgctgctgcc catctaatac gactgcagca atcaaaccaa aaataaccga
    21361 cgcgataatt aatacccatg tgagtagata ctgaatcgcc tgcttcttct gattcttatg
    21421 gattggataa agctgaacga gaaccatata gcggaactgg ttcgctaaag gtaacagttg
    21481 gaaaccgatc aaatataaaa ataagaaacc gataatcaga ctaaaataaa agtcgactac
    21541 tccggcaatc aataacccac ctataattac taatcggaaa taaagaccac taaaatcaga
    21601 acctcttgcc aatcttcttg caaacaagta aagataagtg ttttttgatt ctcttttaat
    21661 atgagtcaat ataggatcca aatagcgtct tcttttgatc tttgccgtaa tttcaggaac
    21721 atctgtgaat aaattgataa attgatagat ctgatgcaac ctatgttctt ctacatgcac
    21781 cattttctcc caatccaatc ctttttcttg ttgcttccac aaaaaccagt aatatagact
    21841 tgcctgtact aatgcacctg ctattcctgc ccatattagc caataaaatg atagaaacaa
    21901 aaagatcaga gaagaaaaaa accagcctaa tttccaataa cgaataactt gttgatctag
    21961 tcgaaacaat tctgctctct tgatttgcag atgactgaat ttcaaactcc ataatatgat
    22021 cagatagacg aaaaacatcg aaaaggattg ctccgtacta accaccacta atggcatggc
    22081 caatccgcat gccaatatca aaaagatcaa cggaaaaatg caagaatagc gaaaagctcg
    22141 ttccaaatag gtttgcattt ctttttcttt aggaagtaaa aaaacaatat ctgctgcctc
    22201 tgcaaaagta gccatcttac ccacgaataa acagagcatc caaaatacag agattatgag
    22261 ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa ttagaataat ataatcccac
    22321 accgccaatc aagaaaagac aaactaaaac aaaatgatcg ttcaatacat aacgcatata
    22381 tttcatcatt ttcttttgat gtcttgctaa acgtttttga aaaaaactac tcatgtgcct
    22441 gctcctttgt taaagcaata tagatttcgt ccaaagaaga atccggcagc tgaaattctt
    22501 ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc gttatgtaag accacaaaac
    22561 gatcacaata tttttctgca gtagctaaaa tatgcgtaga catcaaaata ccggctcctt
    22621 cttttttcat ttctaccatc aattcaagca atgcattgat agctagtgga tccaagccta
    22681 aaaatggttc gtcgatgata tataaagaag gctcgatcaa aaaggcgcat aagatcatca
    22741 ctttctgttt cattcccttt gaaaaattag ctgggaacca gtcaagacga ttagataacc
    22801 gaaaagtctc caacaacttt tctgctcttt tcattgctat atccataggt atatcgtagg
    22861 ccatcgcagt gacttcgata tgttctctca atgtcaattc ttcatataaa gaaggagtct
    22921 ctggaatata cccaatttgt ttgcgatatt cttccgtatt tttctttaag gatagatgat
    22981 tgatttctat cgttcctttt gaagcttcca gcagaccgat aatgtgcttg atggtcgtac
    23041 ttttccctgc tccgttcagt ccaatcaacc ctactatctc tccaggctgt acatcgaaac
    23101 tgatatcttt taacacaggg agatgtccgt acccccctgt gacatgatcg ataattaaac
    23161 tcattctttt tcctccgcca tttattttta tttattatac cacgaagaaa atgacggtat
    23221 tttgtttttt tccttacaat agctttaact tatggtaaag ttaagctgaa gtatttattc
    23281 tttatagaaa ggatgtttta tatggaaaac tgtatttttt gcaaaatcat caatcaagaa
    23341 atcccaagct ataaaatata tgaagacgac aaggtctatg cttttttaga catctctcaa
    23401 gcaacgaaag gacacacatt agttgtgcct aaacgtcatg tagcagatat ttttgaatat
    23461 gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta agattgctca agcattggaa
    23521 aaagcttttc cagaaatgaa aggtcttaat atcttgaata acaaccgtga actagcctat
    23581 caatctgttt ttcattcaca tatccattta gtccctcgct acagcaaaga ggatgatttt
    23641 tctattcact ttgtcaatca tcaagatagt tatggatcgg aagaactaaa agctattcaa
    23701 gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac tttttcaaag gactgttatt
    23761 tggagctgct gctggcactg tcggaggtct gttattggcg cccaggagcg ggaatgaaac
    23821 aagaaagaaa ctgatcgaag aattggaaga agcaacaact ctgacaaatg atttaaataa
    23881 cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa gaaacagctg aatctgtgat
    23941 tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat tttaaattcc aagccgaacc
    24001 acgtatcaca caaatcaatg aacaagtaga aaaactaaaa gctgatctgc cagagtatcc
    24061 tgaacaaata taataatgat tattttctca acagatttaa ctatataagt gagaaaagtt
    24121 tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt ttgaaactac cacgacaacg
    24181 tttttaaagg aaatgggata tggtatagtt aatatataaa aataataact aattacagga
    24241 gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc gttggctgtt
    24301 ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat gaaaggcgga
    24361 acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa ccaatcctta
    24421 gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa agtaaccgat
    24481 aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac ttttgaatca
    24541 caattagaag ctgctggata tacgaaagat acttataaag aatatattcg taacaattta
    24601 gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt gaaaactgct
    24661 tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag tgaagatgaa
    24721 gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt agccaaagac
    24781 aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga ctcaacgaca
    24841 acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg tgagatctcc
    24901 gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt tgtcaaaatg
    24961 gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact aaaagatatc
    25021 gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg tgaagaatta
    25081 aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag tgcattcaca
    25141 acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa gagttctgac
    25201 acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga ttcatcaaaa
    25261 taaa
//
LOCUS       DAZKSR010000038        24852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000038 DAZKSR010000000
VERSION     DAZKSR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.038"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(323..1105)
                     /locus_tag="ACG8XG_001381"
     CDS             complement(323..1105)
                     /locus_tag="ACG8XG_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773305.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1170..2006)
                     /locus_tag="ACG8XG_001382"
     CDS             complement(1170..2006)
                     /locus_tag="ACG8XG_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0773306.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2024..3433)
                     /locus_tag="ACG8XG_001383"
     CDS             complement(2024..3433)
                     /locus_tag="ACG8XG_001383"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0773307.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3447..5270)
                     /locus_tag="ACG8XG_001384"
     CDS             complement(3447..5270)
                     /locus_tag="ACG8XG_001384"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0773308.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6113..6439)
                     /locus_tag="ACG8XG_001385"
     CDS             complement(6113..6439)
                     /locus_tag="ACG8XG_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773309.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6485..6847)
                     /locus_tag="ACG8XG_001386"
     CDS             complement(6485..6847)
                     /locus_tag="ACG8XG_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0773310.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6884..7098)
                     /locus_tag="ACG8XG_001387"
                     /pseudo
     CDS             complement(6884..7098)
                     /locus_tag="ACG8XG_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7226..7561
                     /locus_tag="ACG8XG_001388"
     CDS             7226..7561
                     /locus_tag="ACG8XG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773311.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7637..9634)
                     /locus_tag="ACG8XG_001389"
     CDS             complement(7637..9634)
                     /locus_tag="ACG8XG_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773312.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9732..10007)
                     /locus_tag="ACG8XG_001390"
     CDS             complement(9732..10007)
                     /locus_tag="ACG8XG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773313.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10239..10973
                     /locus_tag="ACG8XG_001391"
     CDS             10239..10973
                     /locus_tag="ACG8XG_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773314.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11012..12154
                     /locus_tag="ACG8XG_001392"
     CDS             11012..12154
                     /locus_tag="ACG8XG_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0773315.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12249..13640)
                     /gene="sufB"
                     /locus_tag="ACG8XG_001393"
     CDS             complement(12249..13640)
                     /gene="sufB"
                     /locus_tag="ACG8XG_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0773316.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13645..14115)
                     /gene="sufU"
                     /locus_tag="ACG8XG_001394"
     CDS             complement(13645..14115)
                     /gene="sufU"
                     /locus_tag="ACG8XG_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0773317.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14102..15337)
                     /locus_tag="ACG8XG_001395"
     CDS             complement(14102..15337)
                     /locus_tag="ACG8XG_001395"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0773318.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15334..16620)
                     /gene="sufD"
                     /locus_tag="ACG8XG_001396"
     CDS             complement(15334..16620)
                     /gene="sufD"
                     /locus_tag="ACG8XG_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0773319.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16633..17406)
                     /gene="sufC"
                     /locus_tag="ACG8XG_001397"
     CDS             complement(16633..17406)
                     /gene="sufC"
                     /locus_tag="ACG8XG_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0773320.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17603..18451)
                     /locus_tag="ACG8XG_001398"
     CDS             complement(17603..18451)
                     /locus_tag="ACG8XG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0773321.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18475..19161)
                     /locus_tag="ACG8XG_001399"
     CDS             complement(18475..19161)
                     /locus_tag="ACG8XG_001399"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0773322.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19154..20188)
                     /locus_tag="ACG8XG_001400"
     CDS             complement(19154..20188)
                     /locus_tag="ACG8XG_001400"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773323.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20251..20507)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20583..20921)
                     /locus_tag="ACG8XG_001401"
     CDS             complement(20583..20921)
                     /locus_tag="ACG8XG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0773324.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20923..21279)
                     /locus_tag="ACG8XG_001402"
     CDS             complement(20923..21279)
                     /locus_tag="ACG8XG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0773325.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21285..22472)
                     /locus_tag="ACG8XG_001403"
     CDS             complement(21285..22472)
                     /locus_tag="ACG8XG_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0773326.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22663..23508)
                     /locus_tag="ACG8XG_001404"
     CDS             complement(22663..23508)
                     /locus_tag="ACG8XG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0773327.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23742..24707
                     /locus_tag="ACG8XG_001405"
     CDS             23742..24707
                     /locus_tag="ACG8XG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0773328.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7264 a         5228 c         4222 g         8138 t
ORIGIN      
        1 ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt ttcataaaaa
       61 aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt cactaaaatc
      121 actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta tctccgtttc
      181 atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac ccccagtagc
      241 ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt agggtaaata
      301 aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg ttgtaatcag
      361 ttacctgtcc gttcttaaat accacagaat aatctttttt cccattttca agtaaccgaa
      421 gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt agattagcta
      481 ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt atcgtgattc
      541 ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt tcgatgaatg
      601 aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta ctcttagatg
      661 aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta ttctcaccgg
      721 aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata gttaaaattc
      781 ccacgctttt actaataaat tcactataac cagttctttc aatagagact tcaatgtatt
      841 tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg tttacatcta
      901 cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct ttactgtctg
      961 atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc tcttctggca
     1021 agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa acattcacct
     1081 ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca acaagtcttt
     1141 atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg cagtgttaga
     1201 taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta ctccttgatt
     1261 ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag caaaaaatca
     1321 tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg taaatgcgtc
     1381 acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa cagatatcct
     1441 agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc catatttgct
     1501 tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc ttctggcaac
     1561 tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc aaattctttt
     1621 gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc tttttgactg
     1681 cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt attcagctta
     1741 aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat tagatcatcc
     1801 ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag tgaatctaat
     1861 ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag ttgaaatcca
     1921 atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag aattaaactg
     1981 ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct aagccatttc
     2041 ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt aattctttaa
     2101 gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag ccttgatggg
     2161 tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca ccatctgtta
     2221 aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc tggtcattaa
     2281 ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta attaaaatag
     2341 gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta gggtcaacta
     2401 cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag acaccttctt
     2461 ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg tcatttttgc
     2521 tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt ttaccggagg
     2581 ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca ttcaaatagt
     2641 tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac cagcctctca
     2701 atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct tgatacatag
     2761 ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga tacaagtgaa
     2821 ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct ttttttgaag
     2881 gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc gtgttttgct
     2941 cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat acctgtacat
     3001 agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc tctagaaaat
     3061 aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc aggcattcat
     3121 caattacttg atgataaaaa gaaattccct tttgattggt tttattattt tcaatatcca
     3181 taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct ttaaacagct
     3241 gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta gctacccctt
     3301 ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga taggcaccct
     3361 ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat gctgaaagct
     3421 gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc gtgttccttt
     3481 aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac taccacaatc
     3541 actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat taataattgt
     3601 cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc ttttaaattc
     3661 acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg tagaccaatc
     3721 gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata aatgctgttt
     3781 tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac agcatcgttc
     3841 acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac tgcttcggct
     3901 attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata agcaatgata
     3961 aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt cattggattt
     4021 tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata tctgactaaa
     4081 ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc aactaatggt
     4141 tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat taaagcagtg
     4201 atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc ggctacagca
     4261 aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt gatcatttca
     4321 gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc tgtacccgta
     4381 accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc tgctttgccg
     4441 ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa cggtccaatg
     4501 acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt aaagaattta
     4561 ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat ccaaacagta
     4621 aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat tcctagaaaa
     4681 gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa aatgccagca
     4741 acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata ggctaatagt
     4801 actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta agtttgacta
     4861 ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac catcccggca
     4921 cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat caatcttgaa
     4981 ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc cacttccttt
     5041 tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta ttgtccaccg
     5101 ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc ggctttcctt
     5161 ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac cacattttct
     5221 tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat tattctctct
     5281 ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg aagtgttagt
     5341 ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt gctgtaatcc
     5401 aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat cttttcgttt
     5461 gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct gcgtatactt
     5521 actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc tacataacca
     5581 gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc acacaacttc
     5641 aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg aataaacagc
     5701 attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat cagtaagcaa
     5761 tagccaattt caatcattcc gagatataaa ggtatcgata gaatgatttg ggctagtatt
     5821 cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat gtagtaagta
     5881 tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat tttttctttt
     5941 tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca atcattgtaa
     6001 tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa ctccctagtg
     6061 aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta aatcaattta
     6121 acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt ggatagtact
     6181 caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg tatgtgttat
     6241 ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg tactgttctt
     6301 gtaaaatctg tgttaccaat ggtttcgatg acttctctaa tccagatgta cctgtcattt
     6361 ttgaaaaaat gatacctata atagccaaga caacgataac tataatgata aacttcttgt
     6421 tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgc tcttatccct
     6481 cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct acccctgcta
     6541 tcatcaaaat cgtacccatt tgttcatctt tatctcgata taagtagccc ataataacag
     6601 caacagcacc aaatacaaca ggaataaatg ctaacgctac taccatagaa atccaaccaa
     6661 taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc atttcattaa
     6721 ttgtctgctc agtacttttc tttactgcgt ctgctccagt tgtctcatct gtttcttgta
     6781 gtgctccgca aaactggcaa tacttacttt catctgttat ttctttgcca cattttctac
     6841 aatacatgtc ttctcctcct aaaaacattc aattgtttat tagttaactg tttactttat
     6901 taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa agcatcttca
     6961 tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat tgctgtgtaa
     7021 cattcatttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt atcggctgag
     7081 attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca atatgatgta
     7141 tttcaactca taaaatataa aaaaacttca aataccatta caaatacaac aacttgttgt
     7201 actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg tcattagaga
     7261 aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct tctctacaca
     7321 acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata gcttaaccgc
     7381 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg tcgtagatta
     7441 tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag ttcgaacaat
     7501 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     7561 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     7621 gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt actctccgtc
     7681 catcatctct tgttacactt gtaaattgat aaagaggaac taattcaact cctgataata
     7741 tcaattcttt tattacttca tctatccctg ctccttttgc taggtagact aaaaaactac
     7801 tacttgcttt cacattgtat agtctcttaa tatccttaga agctcgttca agagtgaatc
     7861 cttgtttaac aaagtagcct ctgttttctt ttaaagcatt ttccacgctg ttattcccaa
     7921 cattacgtag acgttcacaa acagtgttag taagttcttt ctctgtcatt agatatgttc
     7981 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
     8041 tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
     8101 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg
     8161 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
     8221 agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt aacctattta
     8281 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
     8341 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaaa
     8401 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
     8461 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
     8521 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
     8581 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
     8641 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
     8701 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
     8761 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
     8821 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
     8881 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
     8941 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
     9001 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
     9061 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
     9121 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
     9181 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
     9241 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
     9301 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
     9361 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
     9421 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
     9481 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
     9541 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tctttatcta
     9601 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
     9661 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
     9721 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
     9781 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
     9841 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
     9901 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
     9961 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    10021 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    10081 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    10141 cataatacct cgataaaaaa gaggtgtcaa tatctttgta aaatttattt acaacacccc
    10201 tttttcgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa
    10261 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
    10321 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa
    10381 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
    10441 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
    10501 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
    10561 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
    10621 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
    10681 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
    10741 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
    10801 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
    10861 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
    10921 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
    10981 tactccttgt aaactaagag gagatgattt catggcaaaa atatcaaagt acaaaaagaa
    11041 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
    11101 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
    11161 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
    11221 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
    11281 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
    11341 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
    11401 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
    11461 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
    11521 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
    11581 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
    11641 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
    11701 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
    11761 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
    11821 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
    11881 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
    11941 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
    12001 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
    12061 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
    12121 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
    12181 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
    12241 taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact
    12301 gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca
    12361 gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt
    12421 tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg
    12481 atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc
    12541 gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct
    12601 tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt
    12661 gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt
    12721 gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt
    12781 ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg
    12841 tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta
    12901 aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct
    12961 tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc
    13021 gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt
    13081 ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg
    13141 tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa
    13201 gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg
    13261 tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct
    13321 ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga
    13381 gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg
    13441 tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat
    13501 tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc
    13561 aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct
    13621 tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc
    13681 ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat
    13741 ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc
    13801 tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc
    13861 tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca
    13921 gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc
    13981 gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct
    14041 tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa
    14101 tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt
    14161 cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa
    14221 ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt
    14281 ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg
    14341 tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg
    14401 gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt
    14461 gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt
    14521 cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt
    14581 cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac
    14641 caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg
    14701 cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc
    14761 cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg
    14821 atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca
    14881 tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg
    14941 ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt
    15001 ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg
    15061 taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt
    15121 ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa
    15181 cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt
    15241 gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga
    15301 taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac
    15361 ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa
    15421 tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta
    15481 catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc
    15541 atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg
    15601 actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa
    15661 ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg
    15721 ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac
    15781 gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac
    15841 atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc
    15901 acggcgattc atataagaag taatattttc tcctaattga tctacggctg aatatttgat
    15961 ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg
    16021 ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat
    16081 caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag
    16141 tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc
    16201 tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc
    16261 ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt
    16321 ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac
    16381 aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt
    16441 ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg
    16501 cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc
    16561 tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat
    16621 tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc
    16681 agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg
    16741 gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg
    16801 ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac
    16861 tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc
    16921 taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt
    16981 cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt
    17041 tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc
    17101 acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa
    17161 taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa
    17221 aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga
    17281 ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt
    17341 tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac
    17401 agacatgcat tataccccca aattctcttt cgttctcact cattttagac taattgagaa
    17461 tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat taaacaattt
    17521 tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac agacttattt
    17581 ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta acagagccgc
    17641 cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca atcagttttt
    17701 tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg taaggagagt
    17761 tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg taagcgaagt
    17821 ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct aaaagtgatg
    17881 ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca tcgaatgttg
    17941 ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt agaattgtga
    18001 tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca tcttttaatt
    18061 cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg acagcaccgg
    18121 cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga atatgctgga
    18181 agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca tagtcatcga
    18241 atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca tgttccaaaa
    18301 tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg
    18361 ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg
    18421 ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat cctattatct
    18481 tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata cgattagtaa
    18541 aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc cttcctgcca
    18601 agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat
    18661 cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag gtatcaagac
    18721 cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca gtacaccagg
    18781 gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg cggataaaac
    18841 aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt tagtaaatgg
    18901 aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac tgatgatcga
    18961 tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag agtaaaggat
    19021 caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg tcatatatag
    19081 tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt
    19141 ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt tctttgatga
    19201 acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc aatgaaccaa
    19261 aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg atgttatagc
    19321 gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag gtcaaagttg
    19381 ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc gaatcagctt
    19441 ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt tgtctgaaga
    19501 cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg actttgttgc
    19561 agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa ttcaatttct
    19621 tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc aatgcgctag
    19681 tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg ataccgacgc
    19741 gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct tctaacccga
    19801 ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg ccagctaatt
    19861 ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg aaatgctgga
    19921 agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat tttgtaatat
    19981 cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc aggagacgga
    20041 ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc cctttttcaa
    20101 cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg aatgtctttt
    20161 tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat aatttttcaa
    20221 acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt
    20281 accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg gacttagctt
    20341 ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct tacgctcgct
    20401 tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag caaacagggc
    20461 tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt tcatatagga
    20521 ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt gattttctga
    20581 ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat ccacgcattc
    20641 atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt aacagaagaa
    20701 actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc aacttcgatc
    20761 agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa cgtgatgtct
    20821 ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc attaaataag
    20881 atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca gttctccttc
    20941 ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga tcagtggtcg
    21001 cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg taaactcgcc
    21061 tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga acaggcgacg
    21121 gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt
    21181 gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt tggctttttt
    21241 gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct ccttgaagat
    21301 cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc gatcatgttc
    21361 cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt taaaggaagt
    21421 aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat gatcaaaccg
    21481 cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat catacgatag
    21541 attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa gttttcaccg
    21601 ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct tacgttgaat
    21661 cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg ctgataactc
    21721 attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa ctgataagat
    21781 ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt
    21841 cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca agagattcca
    21901 gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc aaagtcatct
    21961 tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc aatcagccaa
    22021 aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac gatcagtgcc
    22081 atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat
    22141 cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa tttgagcaag
    22201 agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca gatccatttg
    22261 gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca gacaacttgc
    22321 atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc tacatataat
    22381 gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata atcaatacga
    22441 ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta aaaaccatac
    22501 tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga catttgggaa
    22561 aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc ctactgctta
    22621 tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc tgagattctc
    22681 ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg caatctctag
    22741 cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat ggaaattcaa
    22801 cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc gaccgattcc
    22861 ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct tatcttggat
    22921 attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg cttctcttac
    22981 aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca tcaaagagta
    23041 tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt
    23101 cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat gcactttagg
    23161 aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa aggtgtggga
    23221 agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt gctgagtttc
    23281 ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt gcttagttgg
    23341 aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc gctccaactg
    23401 tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg ctctggaaaa
    23461 atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg caccacttcc
    23521 ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa aaaaccatct
    23581 ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga tgaagacaat
    23641 cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta ttcgttcatc
    23701 ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac tttttgaaaa
    23761 ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga aatctgttta
    23821 aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg attcttatga
    23881 tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg ttgcatgatc
    23941 caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt tgtacttatt
    24001 ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc gctgttatct
    24061 tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct gatctttcaa
    24121 ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc atcactgtaa
    24181 ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca ttgatctgct
    24241 atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat atggctcatc
    24301 cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta acaggttatc
    24361 ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct ttatctcaag
    24421 tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt ttttatccaa
    24481 gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt actactgttc
    24541 attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa gttgcggaat
    24601 gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt ttcttaatta
    24661 aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa tcgattcaat
    24721 aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa aacagctgtt
    24781 catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac accgtttacc
    24841 aagaggtcgt aa
//
LOCUS       DAZKSR010000039        24735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000039 DAZKSR010000000
VERSION     DAZKSR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.039"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..2187)
                     /locus_tag="ACG8XG_001406"
     CDS             complement(43..2187)
                     /locus_tag="ACG8XG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0773329.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="ACG8XG_001407"
     CDS             complement(2544..3917)
                     /locus_tag="ACG8XG_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0773330.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="ACG8XG_001408"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="ACG8XG_001408"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0773331.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="ACG8XG_001409"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="ACG8XG_001409"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0773332.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="ACG8XG_001410"
     CDS             complement(5365..6378)
                     /locus_tag="ACG8XG_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0773333.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="ACG8XG_001411"
     CDS             complement(6459..7058)
                     /locus_tag="ACG8XG_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0773334.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="ACG8XG_001412"
     CDS             7244..7945
                     /locus_tag="ACG8XG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773335.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="ACG8XG_001413"
     CDS             7955..10666
                     /locus_tag="ACG8XG_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0773336.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="ACG8XG_001414"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="ACG8XG_001414"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0773337.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="ACG8XG_001415"
     CDS             complement(12805..13986)
                     /locus_tag="ACG8XG_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0773338.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="ACG8XG_001416"
     CDS             14238..15761
                     /locus_tag="ACG8XG_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0773339.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="ACG8XG_001417"
     CDS             complement(15830..16558)
                     /locus_tag="ACG8XG_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0773340.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="ACG8XG_001418"
     CDS             complement(16571..17851)
                     /locus_tag="ACG8XG_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0773341.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="ACG8XG_001419"
     CDS             complement(17992..18594)
                     /locus_tag="ACG8XG_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0773342.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="ACG8XG_001420"
     CDS             18839..19045
                     /locus_tag="ACG8XG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773343.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="ACG8XG_001421"
     CDS             19357..20076
                     /locus_tag="ACG8XG_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0773344.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="ACG8XG_001422"
     CDS             complement(20157..20840)
                     /locus_tag="ACG8XG_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0773345.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="ACG8XG_001423"
     CDS             21058..23244
                     /locus_tag="ACG8XG_001423"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0773346.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23590..24000
                     /locus_tag="ACG8XG_001424"
     CDS             23590..24000
                     /locus_tag="ACG8XG_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0773347.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24014..24370
                     /locus_tag="ACG8XG_001425"
     CDS             24014..24370
                     /locus_tag="ACG8XG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0773348.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8264 a         4400 c         4829 g         7242 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt
    23401 gtcacacctc ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat
    23461 agaaaaattc tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta
    23521 ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg
    23581 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
    23641 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
    23701 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
    23761 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
    23821 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
    23881 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
    23941 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
    24001 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
    24061 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
    24121 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
    24181 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
    24241 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
    24301 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
    24361 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
    24421 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
    24481 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
    24541 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
    24601 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
    24661 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
    24721 ttcaaaaaag ttctt
//
LOCUS       DAZKSR010000040        24317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000040 DAZKSR010000000
VERSION     DAZKSR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.040"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..817)
                     /locus_tag="ACG8XG_001426"
     CDS             complement(<1..817)
                     /locus_tag="ACG8XG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0773349.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTRE"
     gene            complement(1041..2981)
                     /gene="thrS"
                     /locus_tag="ACG8XG_001427"
     CDS             complement(1041..2981)
                     /gene="thrS"
                     /locus_tag="ACG8XG_001427"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0773350.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3318..3497)
                     /locus_tag="ACG8XG_001428"
     CDS             complement(3318..3497)
                     /locus_tag="ACG8XG_001428"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0773351.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3544..5367)
                     /locus_tag="ACG8XG_001429"
     CDS             complement(3544..5367)
                     /locus_tag="ACG8XG_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0773352.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5645..7924
                     /gene="helD"
                     /locus_tag="ACG8XG_001430"
     CDS             5645..7924
                     /gene="helD"
                     /locus_tag="ACG8XG_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0773353.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8017..8664)
                     /locus_tag="ACG8XG_001431"
     CDS             complement(8017..8664)
                     /locus_tag="ACG8XG_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773354.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8843..9562)
                     /locus_tag="ACG8XG_001432"
     CDS             complement(8843..9562)
                     /locus_tag="ACG8XG_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0773355.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9693..10844)
                     /locus_tag="ACG8XG_001433"
     CDS             complement(9693..10844)
                     /locus_tag="ACG8XG_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0773356.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11061..11801)
                     /locus_tag="ACG8XG_001434"
     CDS             complement(11061..11801)
                     /locus_tag="ACG8XG_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0773357.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11805..12128)
                     /gene="rsfS"
                     /locus_tag="ACG8XG_001435"
     CDS             complement(11805..12128)
                     /gene="rsfS"
                     /locus_tag="ACG8XG_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0773358.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12185..12778)
                     /gene="yqeK"
                     /locus_tag="ACG8XG_001436"
     CDS             complement(12185..12778)
                     /gene="yqeK"
                     /locus_tag="ACG8XG_001436"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0773359.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12762..13412)
                     /locus_tag="ACG8XG_001437"
     CDS             complement(12762..13412)
                     /locus_tag="ACG8XG_001437"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0773360.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13422..13733)
                     /gene="yhbY"
                     /locus_tag="ACG8XG_001438"
     CDS             complement(13422..13733)
                     /gene="yhbY"
                     /locus_tag="ACG8XG_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0773361.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13749..14858)
                     /gene="yqeH"
                     /locus_tag="ACG8XG_001439"
     CDS             complement(13749..14858)
                     /gene="yqeH"
                     /locus_tag="ACG8XG_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0773362.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14855..15382)
                     /locus_tag="ACG8XG_001440"
     CDS             complement(14855..15382)
                     /locus_tag="ACG8XG_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0773363.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15562..16347)
                     /locus_tag="ACG8XG_001441"
     CDS             complement(15562..16347)
                     /locus_tag="ACG8XG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0773364.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16340..17209)
                     /gene="accD"
                     /locus_tag="ACG8XG_001442"
     CDS             complement(16340..17209)
                     /gene="accD"
                     /locus_tag="ACG8XG_001442"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0773365.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17210..18589)
                     /locus_tag="ACG8XG_001443"
     CDS             complement(17210..18589)
                     /locus_tag="ACG8XG_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0773366.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18597..19016)
                     /gene="fabZ"
                     /locus_tag="ACG8XG_001444"
     CDS             complement(18597..19016)
                     /gene="fabZ"
                     /locus_tag="ACG8XG_001444"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0773367.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19041..19517)
                     /gene="accB"
                     /locus_tag="ACG8XG_001445"
     CDS             complement(19041..19517)
                     /gene="accB"
                     /locus_tag="ACG8XG_001445"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0773368.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19520..20755)
                     /gene="fabF"
                     /locus_tag="ACG8XG_001446"
     CDS             complement(19520..20755)
                     /gene="fabF"
                     /locus_tag="ACG8XG_001446"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0773369.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20774..21511)
                     /gene="fabG"
                     /locus_tag="ACG8XG_001447"
     CDS             complement(20774..21511)
                     /gene="fabG"
                     /locus_tag="ACG8XG_001447"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0773370.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21523..22440)
                     /gene="fabD"
                     /locus_tag="ACG8XG_001448"
     CDS             complement(21523..22440)
                     /gene="fabD"
                     /locus_tag="ACG8XG_001448"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0773371.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22547..22771)
                     /locus_tag="ACG8XG_001449"
     CDS             complement(22547..22771)
                     /locus_tag="ACG8XG_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0773372.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22824..23786)
                     /locus_tag="ACG8XG_001450"
     CDS             complement(22824..23786)
                     /locus_tag="ACG8XG_001450"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0773373.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23796..24236)
                     /locus_tag="ACG8XG_001451"
     CDS             complement(23796..24236)
                     /locus_tag="ACG8XG_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0773374.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6645 a         5425 c         4239 g         8008 t
ORIGIN      
        1 cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa
       61 aaatgatatc gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg
      121 tgatttcttt tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat
      181 ttccaaattg cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg
      241 cgactacatc tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat
      301 tgctgacaag ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga
      361 cacgagacca tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata
      421 aaaaaacagt ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta
      481 gaatatctac tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag
      541 agtaaaagga ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt
      601 ctgttgaaat cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac
      661 gtacaactgt cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag
      721 ggaccttctc aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat
      781 cttccatcat actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga
      841 tagtctatgt aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac
      901 aaaaaaacaa tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc
      961 atctgtgtct ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt
     1021 ttttacagga aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata
     1081 gcatcaataa agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg
     1141 ttcactgttg catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt
     1201 gtttgagagg cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc
     1261 aaccccttca tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca
     1321 gaaacaggga tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat
     1381 acttctgtta aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact
     1441 actggacgat gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc
     1501 aataagaagt ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact
     1561 tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt
     1621 tctaattcat ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca
     1681 tcaaaatatt tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta
     1741 atattgaagt cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg
     1801 atttgatcag gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt
     1861 aaacctgaca aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga
     1921 attggtaatt cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag
     1981 ttcattgggc gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct
     2041 tcttgataat gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt
     2101 gtatatacat gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg
     2161 cgtcggattg ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa
     2221 accatgaaca agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca
     2281 cggagacgga tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt
     2341 tgcatcattt gattgtttga gtttccacgc caataagcac ctgccactga taataattta
     2401 aatacttgta tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg
     2461 ccttgttgat aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg
     2521 tacggatcac ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc
     2581 ggcagatttt ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca
     2641 gttacttggt tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca
     2701 acgccaaagt ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct
     2761 gaatgacgga ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca
     2821 tcttcctcga ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct
     2881 ttttttgcta agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca
     2941 aattctttga ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa
     3001 tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa
     3061 gagatactag gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc
     3121 aagcgttgta acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt
     3181 tggttttctc ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat
     3241 tcagttggct ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat
     3301 caagtgggaa actaaactta ttttttccat tccccattta caccgtatgt tccttttttc
     3361 atctcattga tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct
     3421 tccgtgatat ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg
     3481 acatgtacga atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt
     3541 ctatcatact atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc
     3601 tctcaaggca ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa
     3661 tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat
     3721 cagtacagac tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac
     3781 acgcaagaga tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc
     3841 atgagccccc attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc
     3901 gtctcctacc atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt
     3961 atcttgaggc aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg
     4021 agcaatggca gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag
     4081 ctccttgatt gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc
     4141 aaaagatcga tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga
     4201 aacaaaagag gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg
     4261 accggtcact tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac
     4321 taacgaacga gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag
     4381 ctcttctttt tttattccat tgactggctg aatcgcctct acttccaata tccctttggt
     4441 aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt
     4501 tccagttttt acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat
     4561 gggcgcagct agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa
     4621 agggtctttt gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg
     4681 gactgcataa cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac
     4741 tagtttaata atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat
     4801 cgatccgtct ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg
     4861 gcgggactca cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc
     4921 atctgcgggt acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc
     4981 gattactata tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc
     5041 taacaaagac tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc
     5101 gcccgtaagc atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc
     5161 aaggattgcg gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat
     5221 catttccaaa aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc
     5281 aagggccggc tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt
     5341 aagtaaaaat tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag
     5401 gttagtaata aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt
     5461 ttaaaacaat aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca
     5521 gttctacaaa caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac
     5581 cttttttcga aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca
     5641 tgcgatgaat gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca
     5701 gatcgaacaa aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc
     5761 gacaaaagac acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc
     5821 tgctgttgaa tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc
     5881 acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat
     5941 cgactttaaa gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga
     6001 tctggcagaa gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga
     6061 aggtgagatt ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca
     6121 gttgaaacga caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga
     6181 agtaatcaat gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa
     6241 aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa
     6301 agtaatgctg attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat
     6361 tgcttttctg ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc
     6421 acccaaccat ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg
     6481 cgtgcctaca cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact
     6541 agatgaacag caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat
     6601 gaaaagcgga ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta
     6661 cggtcccttg ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat
     6721 tcgccaatgg tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt
     6781 acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg
     6841 ggtcaaagat ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga
     6901 atatacagaa caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa
     6961 aagattccgt cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca
     7021 atatctccac tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga
     7081 gaccaggtgg caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg
     7141 tcaagaggat gctgtactat tctttttact gatgcgccga acatatccag tgacggttac
     7201 acacaaagct cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc
     7261 attgctgcaa gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa
     7321 tgtctttgga aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt
     7381 cactcgtttc caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa
     7441 tcattttatt gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt
     7501 gctcatccaa ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac
     7561 gactcaggga aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg
     7621 cgaagctctt tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa
     7681 tgagagtgac tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct
     7741 ggagtttgac cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga
     7801 caaattgatc ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc
     7861 caataatgaa agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact
     7921 gtaagtaaaa agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat
     7981 aattgcagaa aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc
     8041 tgtttcagga tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc
     8101 tggatattct gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg
     8161 gtaaattcga ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc
     8221 gccacaatcc aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt
     8281 ttttttcgtg tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa
     8341 gctccactct aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa
     8401 ggctgaatca gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat
     8461 tggtgcgcgg tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt
     8521 tcctaatcga tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc
     8581 agttacgttc acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg
     8641 ttctaaagtg atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca
     8701 tttcacatga tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc
     8761 acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat
     8821 acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta
     8881 catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag
     8941 atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc
     9001 ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga
     9061 tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa
     9121 ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag
     9181 aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg
     9241 ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc
     9301 caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga
     9361 ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac
     9421 gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa
     9481 tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga
     9541 tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg
     9601 caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc
     9661 ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa
     9721 ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc
     9781 gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt
     9841 aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg
     9901 acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt
     9961 gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt
    10021 gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt
    10081 tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc
    10141 tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt
    10201 tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg
    10261 attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca
    10321 tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca
    10381 agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac
    10441 gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag
    10501 cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca
    10561 cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc
    10621 gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg
    10681 cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca
    10741 gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca
    10801 ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat
    10861 agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc
    10921 cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa
    10981 gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt
    11041 aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc
    11101 tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt
    11161 tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca
    11221 cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg
    11281 ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa
    11341 tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa
    11401 aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt
    11461 ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt
    11521 tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct
    11581 cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca
    11641 tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt
    11701 gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc
    11761 atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca
    11821 ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct
    11881 ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct
    11941 tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct
    12001 tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc
    12061 aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct
    12121 gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag
    12181 cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt
    12241 gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta
    12301 gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa
    12361 tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat
    12421 gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg
    12481 aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac
    12541 cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg
    12601 tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg
    12661 ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt
    12721 tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc atattcatac
    12781 aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag
    12841 ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc
    12901 caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg
    12961 accatagaag tcagctcatc gattttatac cattttggca aatattctac catatcccca
    13021 ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca
    13081 taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg
    13141 tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca
    13201 tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg
    13261 ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag
    13321 atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa
    13381 cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct
    13441 tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca
    13501 atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca
    13561 atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct
    13621 tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga
    13681 agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc
    13741 cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc
    13801 gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt
    13861 ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga
    13921 cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt
    13981 ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg
    14041 ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga
    14101 taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag
    14161 tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca
    14221 tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca
    14281 tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt
    14341 gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt
    14401 tccgtcatct catgagattt cttagcacta gttaataata catctactgg acgaattcct
    14461 gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt
    14521 aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt
    14581 cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct
    14641 ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg
    14701 gtctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa
    14761 cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata
    14821 acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca
    14881 tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt
    14941 gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac
    15001 gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg
    15061 ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg
    15121 caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg
    15181 aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt
    15241 ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt
    15301 tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct accatccatg
    15361 ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa
    15421 agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt
    15481 tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa
    15541 aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa
    15601 tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat
    15661 gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc
    15721 aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc
    15781 ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata
    15841 aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact
    15901 tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag
    15961 atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc
    16021 tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg
    16081 taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg
    16141 cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat
    16201 tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga
    16261 atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg
    16321 aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa
    16381 gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat
    16441 gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt
    16501 cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc
    16561 cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat
    16621 aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta
    16681 gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa
    16741 gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca
    16801 tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg
    16861 ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt
    16921 tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt
    16981 caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt
    17041 atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc
    17101 aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct
    17161 ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc
    17221 ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt
    17281 aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga
    17341 tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac
    17401 gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat
    17461 atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt
    17521 tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc
    17581 attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg
    17641 ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat
    17701 gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca
    17761 gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt
    17821 ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt
    17881 tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc
    17941 gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca
    18001 tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga
    18061 gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca
    18121 gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata
    18181 caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga
    18241 tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact
    18301 cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg
    18361 cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa
    18421 cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg
    18481 catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga
    18541 tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat
    18601 ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct
    18661 ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct
    18721 cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt
    18781 cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca
    18841 atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg
    18901 atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca
    18961 atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt
    19021 ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg
    19081 acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc
    19141 tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca
    19201 cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt
    19261 ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga
    19321 gcagttgttg cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt
    19381 gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga
    19441 ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg
    19501 atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc
    19561 cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt
    19621 tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa
    19681 attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata
    19741 aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg
    19801 cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat
    19861 tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt
    19921 tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac
    19981 cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt
    20041 caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg
    20101 gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag
    20161 caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc
    20221 catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta
    20281 cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt
    20341 tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac
    20401 gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa
    20461 accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag
    20521 cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt
    20581 tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa
    20641 atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg
    20701 cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt
    20761 tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg
    20821 tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga
    20881 aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg
    20941 tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag
    21001 cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg
    21061 cttgaccaat atttccaatc aacccagata cactagagag attaatgatg gccccttctc
    21121 gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga
    21181 tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga
    21241 ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca
    21301 tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga
    21361 atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat
    21421 ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg
    21481 tgataaatac attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt
    21541 ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt
    21601 cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt
    21661 ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag
    21721 tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga
    21781 aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa
    21841 aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc
    21901 ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg
    21961 aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc
    22021 ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc
    22081 agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag
    22141 cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg
    22201 gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg
    22261 ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc
    22321 gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa
    22381 atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat
    22441 gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac
    22501 attcctattt caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt
    22561 tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca
    22621 cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta
    22681 agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg
    22741 cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct
    22801 ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct
    22861 ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa
    22921 actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata tttatccata
    22981 ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc aataattctg
    23041 aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata
    23101 ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa
    23161 tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc
    23221 aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga
    23281 ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca
    23341 gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg
    23401 cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg
    23461 atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat
    23521 ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt
    23581 agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga
    23641 tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta
    23701 tccatcatct tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt
    23761 gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg
    23821 tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag
    23881 atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt
    23941 ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag
    24001 ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct
    24061 ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc
    24121 tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa
    24181 atatcattga agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc
    24241 caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt
    24301 ttttcaaaat gcaacct
//
LOCUS       DAZKSR010000041        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000041 DAZKSR010000000
VERSION     DAZKSR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.041"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8XG_001452"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8XG_001452"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0773375.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8XG_001453"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8XG_001453"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0773376.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8XG_001454"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8XG_001454"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0773377.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG8XG_001455"
     CDS             complement(3374..4681)
                     /locus_tag="ACG8XG_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0773378.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG8XG_001456"
     CDS             complement(4684..5265)
                     /locus_tag="ACG8XG_001456"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0773379.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG8XG_001457"
     CDS             complement(5837..7129)
                     /locus_tag="ACG8XG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0773380.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG8XG_001458"
     CDS             complement(7126..7749)
                     /locus_tag="ACG8XG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773381.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG8XG_001459"
     CDS             complement(7752..8588)
                     /locus_tag="ACG8XG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0773382.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG8XG_001460"
     CDS             complement(8605..9492)
                     /locus_tag="ACG8XG_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0773383.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG8XG_001461"
     CDS             complement(9508..10791)
                     /locus_tag="ACG8XG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0773384.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG8XG_001462"
     CDS             complement(11474..12157)
                     /locus_tag="ACG8XG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0773385.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG8XG_001463"
     CDS             complement(12175..12792)
                     /locus_tag="ACG8XG_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0773386.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG8XG_001464"
     CDS             complement(12802..13626)
                     /locus_tag="ACG8XG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0773387.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG8XG_001465"
     CDS             complement(13619..14179)
                     /locus_tag="ACG8XG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773388.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG8XG_001466"
     CDS             14353..15309
                     /locus_tag="ACG8XG_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0773389.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG8XG_001467"
     CDS             complement(15614..16570)
                     /locus_tag="ACG8XG_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0773390.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG8XG_001468"
     CDS             16674..17276
                     /locus_tag="ACG8XG_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0773391.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG8XG_001469"
     CDS             complement(17364..18863)
                     /locus_tag="ACG8XG_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0773392.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG8XG_001470"
     CDS             complement(18879..19403)
                     /locus_tag="ACG8XG_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0773393.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG8XG_001471"
     CDS             19701..20450
                     /locus_tag="ACG8XG_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0773394.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG8XG_001472"
     CDS             20441..21226
                     /locus_tag="ACG8XG_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0773395.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG8XG_001473"
     CDS             21195..21911
                     /locus_tag="ACG8XG_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0773396.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG8XG_001474"
     CDS             complement(22013..23032)
                     /locus_tag="ACG8XG_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0773397.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG8XG_001475"
     CDS             complement(23051..24052)
                     /locus_tag="ACG8XG_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0773398.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKSR010000042        23830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000042 DAZKSR010000000
VERSION     DAZKSR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.042"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8XG_001476"
     CDS             complement(54..788)
                     /locus_tag="ACG8XG_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0773399.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8XG_001477"
     CDS             1115..1525
                     /locus_tag="ACG8XG_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0773400.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8XG_001478"
     CDS             1518..2219
                     /locus_tag="ACG8XG_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0773401.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8XG_001479"
     CDS             2219..3805
                     /locus_tag="ACG8XG_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773402.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8XG_001480"
     CDS             3824..4045
                     /locus_tag="ACG8XG_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0773403.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8XG_001481"
     CDS             4042..4803
                     /locus_tag="ACG8XG_001481"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0773404.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8XG_001482"
     CDS             5183..5692
                     /locus_tag="ACG8XG_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0773405.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8XG_001483"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8XG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0773406.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8XG_001484"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8XG_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0773407.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8XG_001485"
     CDS             7319..8170
                     /locus_tag="ACG8XG_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0773408.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8XG_001486"
     CDS             8198..8833
                     /locus_tag="ACG8XG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0773409.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8XG_001487"
     CDS             8853..9626
                     /locus_tag="ACG8XG_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0773410.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8XG_001488"
     CDS             10034..10831
                     /locus_tag="ACG8XG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0773411.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8XG_001489"
     CDS             10809..11222
                     /locus_tag="ACG8XG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0773412.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8XG_001490"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8XG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0773413.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8XG_001491"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8XG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0773414.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8XG_001492"
     CDS             15999..16559
                     /locus_tag="ACG8XG_001492"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0773415.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8XG_001493"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8XG_001493"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0773416.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8XG_001494"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8XG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0773417.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8XG_001495"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8XG_001495"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0773418.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8XG_001496"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8XG_001496"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0773419.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8XG_001497"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8XG_001497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0773420.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG8XG_001498"
     CDS             21906..22484
                     /locus_tag="ACG8XG_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0773421.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG8XG_001499"
     CDS             22536..23018
                     /locus_tag="ACG8XG_001499"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0773422.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG8XG_001500"
     CDS             23135..23509
                     /locus_tag="ACG8XG_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773423.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DAZKSR010000043        23406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000043 DAZKSR010000000
VERSION     DAZKSR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.043"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1715
                     /locus_tag="ACG8XG_001501"
     CDS             54..1715
                     /locus_tag="ACG8XG_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0773424.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1785..2132)
                     /locus_tag="ACG8XG_001502"
     CDS             complement(1785..2132)
                     /locus_tag="ACG8XG_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0773425.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2216..3157
                     /gene="opp3b"
                     /locus_tag="ACG8XG_001503"
     CDS             2216..3157
                     /gene="opp3b"
                     /locus_tag="ACG8XG_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0773426.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3158..4201
                     /locus_tag="ACG8XG_001504"
     CDS             3158..4201
                     /locus_tag="ACG8XG_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773427.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4207..5277
                     /locus_tag="ACG8XG_001505"
     CDS             4207..5277
                     /locus_tag="ACG8XG_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773428.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5267..6220
                     /locus_tag="ACG8XG_001506"
     CDS             5267..6220
                     /locus_tag="ACG8XG_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773429.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6232..7242
                     /locus_tag="ACG8XG_001507"
     CDS             6232..7242
                     /locus_tag="ACG8XG_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0773430.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7527..8348)
                     /locus_tag="ACG8XG_001508"
     CDS             complement(7527..8348)
                     /locus_tag="ACG8XG_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0773431.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8445..8723)
                     /locus_tag="ACG8XG_001509"
     CDS             complement(8445..8723)
                     /locus_tag="ACG8XG_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773432.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8991..11000
                     /gene="metG"
                     /locus_tag="ACG8XG_001510"
     CDS             8991..11000
                     /gene="metG"
                     /locus_tag="ACG8XG_001510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0773433.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11479..12066)
                     /locus_tag="ACG8XG_001511"
     CDS             complement(11479..12066)
                     /locus_tag="ACG8XG_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773434.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12241..13563
                     /locus_tag="ACG8XG_001512"
     CDS             12241..13563
                     /locus_tag="ACG8XG_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0773435.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13718..14494
                     /locus_tag="ACG8XG_001513"
     CDS             13718..14494
                     /locus_tag="ACG8XG_001513"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0773436.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14487..15056
                     /gene="rnmV"
                     /locus_tag="ACG8XG_001514"
     CDS             14487..15056
                     /gene="rnmV"
                     /locus_tag="ACG8XG_001514"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0773437.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15049..15933
                     /gene="rsmA"
                     /locus_tag="ACG8XG_001515"
     CDS             15049..15933
                     /gene="rsmA"
                     /locus_tag="ACG8XG_001515"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0773438.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16384..17013
                     /locus_tag="ACG8XG_001516"
     CDS             16384..17013
                     /locus_tag="ACG8XG_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773439.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17137..18246)
                     /locus_tag="ACG8XG_001517"
     CDS             complement(17137..18246)
                     /locus_tag="ACG8XG_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773440.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18455..18877
                     /gene="mgsA"
                     /locus_tag="ACG8XG_001518"
     CDS             18455..18877
                     /gene="mgsA"
                     /locus_tag="ACG8XG_001518"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0773441.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19064..19162
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19200..19736
                     /locus_tag="ACG8XG_001519"
     CDS             19200..19736
                     /locus_tag="ACG8XG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0773442.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19776..21539)
                     /locus_tag="ACG8XG_001520"
     CDS             complement(19776..21539)
                     /locus_tag="ACG8XG_001520"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773443.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21542..23257)
                     /locus_tag="ACG8XG_001521"
     CDS             complement(21542..23257)
                     /locus_tag="ACG8XG_001521"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773444.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7533 a         4291 c         4768 g         6814 t
ORIGIN      
        1 caacttcaga aaatattaat tattctgaaa attccaataa aggggtacgt aaaatgaaaa
       61 agaaatttac atttggtttg gttgcggtat gcggccttgt acttgctgga tgttatggcg
      121 gcagttcagc cacaagcagt agttcagcaa aaagcggttc agcagatgga ggaggcgtat
      181 ttaacttagt cgtgcctcaa gaaatgccta cagctgactt atcagttgca acagatacaa
      241 tcagttttac agcattgaat aacgtttatg aaggaattta ccgtcttgat gaagacagca
      301 aaccacagcc ggcaggagct agcgagttgg cagaagtcag tgaagacgga ttaacttaca
      361 aaatcaaatt acgagaagac gcaaaatggt caaatggtga tcctgttaca gcagcggatt
      421 acgtttatgg ctggcaacga acagtagacc cagctacagc ttctgaatat gcttacctgt
      481 atgcgccagt tgaaaatgca gaagacatta cagctggaaa gaaagataaa tcagaactag
      541 ggatcaaagc agttggtgac tatgaattag aaatcaaatt gacgaaacaa acaccatatt
      601 tccaatatct gcttgctttc ccatcatttt tcccacaaaa acaatccgta gtggaagaaa
      661 atggggatgc ctatgcatct gcaagtgata aagctgttta taatggacca ttcacattag
      721 aagattttga tggaccagga acagatacag aatggactta taagaaaaat gatcattact
      781 gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta
      841 ctgcattgaa cttattcaaa gatggccaag cagatgacgt gatcttgtct ggtgagttag
      901 cacagcaaaa cgcaaacgat ccagcttata cttctgttaa agaagcgcgg acaagctata
      961 tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa tgtgaaccta agaaaagcta
     1021 tttcttattc tatcgatcgt gaagctttag taaaacaagt actaggtgac ggatcagttg
     1081 tctctacagg attgatccca tcagatatgg taaaaagtcc aacaacgaac gaagactttg
     1141 caaaagaagc tggaaaatta gtttcttatg acaaagataa agcaaaagaa tattgggaaa
     1201 aagcgaaaaa agaattgggc gttgattctt tagaatttga tttgatggct tctgatgatg
     1261 attcatcgaa aaaagtaatc gaatatgttc aaaactcaat ccaagaaaac ttggatggcg
     1321 taacagtcaa accaacacct gttccattct ctgtacgtct tgaccgctca acttcgggtg
     1381 attttgacgc agtactaggc ggatgggctg ctgactatgc agacccaagc agctttactg
     1441 atttgtttgt cacaggtaat tcatacaacc gtggtcgctg gagcaatgca gaatatgacg
     1501 aagcagttaa agcagcttca tcaacagacg ctggcgatga acaagcccgt tgggatgatt
     1561 tccaaaaagc aatcaagatc atctctgatg atatgggtgt cgttccggtg taccaaaaag
     1621 cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt
     1681 catgggatta caaatggaca tacgttgaag aataatcgtt aataccgtga gcatgagaaa
     1741 aaacgaggct gcggcaaatg tcgcaacctc gttctcatat tcacttatcc taacaaagaa
     1801 aaaagcatga aaatagcaga aagagcaatt agtattccgg caatgatcag ccaaaagctg
     1861 tacataccgt agctggcaga cgagagcgtg gaaaattctg gttttttctt tcggttcctt
     1921 gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat
     1981 aaaaaaccgc cgataatcag aaacggcaat gaccataaga atagatcatt agataattga
     2041 atgagtgtaa aggaatggtt gatgagattt tttattaaaa taaccagaat cagtagaaaa
     2101 gctactgcgt aggggataga tttttttttc atttgattgc tcctttattt ttggttcatt
     2161 atctaataat gtagcgaaac atctgtcaga agtcaaaagc tagggggaaa cattagtgaa
     2221 tagttatatc aaatatgtat taaaacgtgt ctttttcatg atcatcacgc tttggttaat
     2281 tgcaacaatc acctttttct tgatgcaatt gctgccgggc acgccatata cgaatcagga
     2341 acgtttgagt cctgaaacaa tcgagatgct aaataaacaa gtcggtttgg ataaaccagt
     2401 tatcgtacaa tatgggattt atctttctaa tttgcttcaa ggagattttg gaatttcctt
     2461 ccaatttaaa aaccagccag tcgcacattt gttagctgga agaatcggac catccttgca
     2521 gttagggtta caggcaatta tctttggaac agtcctaggg acgattttag gaacgatctc
     2581 tgccatgaag caaaatacat gggcagatac atcttctaca ctcgtagcga tattaggtcg
     2641 ttccattcca aactttgtat ttgctgtttt attacaatat atctttgcaa tcaaattaca
     2701 agttttgcca atcgcaaaat gggatggctt tgtgtacaca atcttaccaa caattgcgtt
     2761 ggccatgtcg ccgcttgctg actctgctcg gtttatccgt acggaaatgg tggaagtact
     2821 gcatagtgat tatgtggaat tagcaaaagc aaaaggattg agccgttggg aaatcgcctt
     2881 taaacatggt cttcgaaata gtttgattcc attgatgact ttattaggac cgttagctgt
     2941 agcattgatg accggatcac tggtggtaga aaatatcttt gctattcctg gtatcgggga
     3001 acaattcgtt aagtcaatca caacaaatga ttatcctacg atcatggctg taacgatcct
     3061 ttactcattt atgctgattt ttgttatttt gatcgtggat ctattgtacg gtctagttga
     3121 tccaagaatt cgtgtttcag aagggagccg aggataaatg gaaatgatac caaagcgtta
     3181 tgaaacagtt gcagatatcc ctgcggatga atttcttcca ttacaaaaaa ataccgaaga
     3241 agaacgtgaa cagattactg caccttcttt gaattttatc caagattcat ggcgccgttt
     3301 aaagaaaaac aaagctgccg ttatttcaat ggttttacta ttggtcatca ttttgatctc
     3361 tgtgatcaca atctttgttt ctcctcatga tccaactgcc caaaacgtgg cttatatcaa
     3421 tttgcctcct cgtattccgg gaatcaatat cgatggattg aatggaaaag caatggttgg
     3481 tggtgaactt gttgataaat atgctcaagc aaacgtccct tcaaacgtaa atttcttttt
     3541 aggaacagac ggtttgggac gtgatgtatt aagccgctta tttatgggaa tgagaatctc
     3601 gttgctgatc gcatttattg ctgcattact agatgtaacg attggtgtag cttacgggtt
     3661 ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg caacgttttt tggaaatctt
     3721 atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat
     3781 tttttcaatc gtggctgcta tggccatcac aaactggata ccaatggcac gtatcgttcg
     3841 tgcacaaaca atgaaattaa aagatcaaga atatgtttta gctggattga cattaggtga
     3901 atcaagaatg aaaattgcat tcaaacatat tttgccgaat atctctagtg tcattattat
     3961 tcaaatgatg tttagtattc catctgctat tttcttcgaa gcctttttaa gctttatcgg
     4021 tttaggtctt acgcctccgt cagcttcatt aggaacgatg ttgagcgaag ggtacaagac
     4081 attcctgtat ctgccttatc tattatggat tccggcagta actttatcgg ttatcatgat
     4141 tggctttaac ttacttgctg atggattgcg tgatgccttt gatcctaaaa tgaaagagtg
     4201 aagacgatga cgaaaatttt agaagtgaaa gacttagaaa tctcttttga tacgtatgct
     4261 ggaaaggtac gagcgattcg cggtgtcaat tttcatttga ataaaggaga aacattggcg
     4321 atcgttgggg aatctggaag tggaaaatca gtgacgacca gaagcatcat gcgcttgctg
     4381 tcaagcaacg ccaatataga tgctggtcaa atcctcttta aaggtaacga tatcgttgga
     4441 aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat
     4501 ccaatgacct ctttagatcc gacgatgccc attgggaagc aagtcgctga gtcccttaga
     4561 aagcataata aagtttcaaa aaaagaggga caaaaagctg cattagaatt gcttaaatta
     4621 gttggaatac ccgaagctga gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt
     4681 cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt atccggaaat cctgattgct
     4741 gatgaaccta caacagcgct tgatgtgact attcaagcac agattttgga gctgctgaaa
     4801 gaaattcaga caaaaatcga tacatcaatt atctttatca ctcatgacct tggtgtagta
     4861 gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa gaatcgttga agttggaaca
     4921 tcggaagaaa ttttctacaa cccgcaacat ccctatactt ggggactgct tggttcaatg
     4981 ccgacattag atagtgcgaa cgatcgattg tatgctattc ctggttctcc accggattta
     5041 ttgaacccgc caaaaggaga cgcattctat ccaagaaatg aattcgcgat gaaaatcgat
     5101 gcagaacaag aaccaccatt ttttgaacta tcaaaaacac acaaggcagc aacttggcta
     5161 cttgctccgc aggcacccaa agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc
     5221 tttaaagaga aacaaaaaca tacttacggt caaggaggaa ttgctgatgc caaataaact
     5281 acttgaagtt aaaggattga aacagtattt caatgtagga aaaaagaatg aagtacatgc
     5341 tgtaaatgac atcagctttc acatttatga aggagaaaca ttcggtttag ttggagaatc
     5401 tggaagcgga aaatccacta ctggccgtac gatcattcgc ttgaatgaac ctactgatgg
     5461 tgagatatta ttcgacggtc aagatataac gaagatcaaa gataaaaaag gtttgacgaa
     5521 attccggcat gatgttcaga tgatctttca agatccatac gcttcgttga atcctcgtat
     5581 gaaagtcaga gatatcattg ccgaaggaat tgatgttaat ggactagcta aatcatctga
     5641 agagcgtgca aaaaaagtcg atgatctatt gagaactgtt ggattgaatc caagccacgg
     5701 tactcgttat cctcatgaat tttcaggtgg gcaacgccag cgtattggta ttgcacgagc
     5761 attggcagta aaaccgcgtt ttatcatttg tgatgaacca atttctgcac tagatgtgtc
     5821 gatccaagcc caagtggtca atttgttgca agatctccaa aaagaacatc aattgaccta
     5881 tctatttatt gcacatgatt tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat
     5941 gcataacggc ttgttattag agatgggaac aagtgatgaa atctataatc acggtgttca
     6001 tccttataca gaaagtttgt tatctgctat tcctctacca gatccagacc atgagagaaa
     6061 acgcagaaga atcaaatatc aacctgaacc agatgacgga caagtacgtc aacttcgtga
     6121 aatcgcacca gaacattttg tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa
     6181 actaaaacga caaaaggaat ctcttctggt tgctaactag gagggacata gatgctaaaa
     6241 gcttttaaat tccgtatgta tccgacagaa gaacaaaaac agcaattgat tcgtactttt
     6301 ggatgtgctc gatttaccta taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa
     6361 acaggagtag ccaatttttc gctcacaccc gctacattaa aaaaagaata cccattctta
     6421 aaagaagtag acagtcttgc tttagcaaat gcacagctta atttggatcg ggcttttcgc
     6481 aattatttca agggacgtgc cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa
     6541 tcttatacga ccaacaacca aacccgtacg atctatctgg aaaatggcta cttaaagtta
     6601 ccaaaacaaa aagaactaat caagataaat cagcatcggc cagttgaagg atcgattcgc
     6661 tccgctacga tttctgctcg atataatgaa gaattttatg tcgcactact ttgtgatgtt
     6721 tcgccagtaa aaaaagaatc tttagctaaa tggatcggga tcgcctatca tccgaagaca
     6781 ttgatccaaa catctcgacc tctagaagta actttaccaa aatttcacca gacagaagaa
     6841 aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc gctccgctca ccatagaaag
     6901 atcaggttag atcaggcaag caattatcag aaacaaaaga gaaaagtaat ggatctttat
     6961 ctgaaacaaa agaaccagcg agaagattat cttgaacaat tatctgggaa attggtcaaa
     7021 caatatgact acttatttgt agaatctttt ccaaaagaag aggcacatgc tgatttttct
     7081 attcacgact ggcataaatt aataacaaaa ttgcgttata agtcccaatg gtataataaa
     7141 aaatttcttt tgatcaatac cgacggagca gaagaaagca attccgtaag aaagagccaa
     7201 gtagtagaaa aaatgggcag acattcggtt atcaagggat aaaaaatgaa tgaaactgat
     7261 aggagcgtta gggttagcct tgttaataaa aagtcttaat gacttttgtt tctaggaatt
     7321 tttcttttga agaaaagctc actaattatg acaaaccaat aaaacaatag cagaaaaagt
     7381 tcgtacgagc tttttctgct attgttaatt ttttattcag aaagtttacg aatacatcaa
     7441 aattctgaaa caaaaaacgt aactcacaaa gagttgtttt tgcgtttttt cttgaatgat
     7501 taggtctgaa tcttttgctc acaaccttat tcatggtgcc ccggcttgag ctgatagatt
     7561 tttgaaggac ggccgattcc tacaggacgt tcaccgatct catcgaaata ttggagcata
     7621 gctttcttaa aattggaatg atcgatcgtt ttataatcga tccctaagaa tttagcaaat
     7681 acttttcgag cttccgtaat cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg
     7741 tgttccattt tattgactac acggttgaat gctttcaaga tgatttgact atggtcaaaa
     7801 gccaaagtat ctttgccaga agactctcca gtgactaaat caagcgagat agccacttct
     7861 ccgctagata aatggatctt gttactatga cgttctagtg tgaaccagcg tacttccttg
     7921 gcatcatctc cggcgattag cggttcttct ccaataaagg ctaagtagct gactgtgacg
     7981 acccagcctc gcggatctcg attaggcgta ctgaatgtat gcagttgctc aatattttct
     8041 tttgaaataa ccacaccagt ttcttccttt gtttctctta agacgctttc tccagttgac
     8101 tcatcctttt ggacaaagcc accaggcaat gcccatgaat ttcgataagg atgccctttt
     8161 ctttggatca acaaaacttt cagctgatct tcttctttgt tatagcacat cagtacgata
     8221 tcgacagtca aagagggctt gtcatattct ggacgttcct gtttttgata ccagttaaga
     8281 aattcttctt cacttgcttt atgttcataa tataattttt cttcttcttt agaagcaaaa
     8341 cggttcatct gaccacctcc attgacagta aaagtacttt ttaccttatt gtagcagact
     8401 ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta tcatttaaat atgaaaaata
     8461 aatggaagta tgagattgaa taaaaagaaa agcagataaa acggcaatac tttataccgc
     8521 tcttcacgaa tggcaaagaa gccacaaata acgatatata cgtcaatgat aaaacgcaaa
     8581 atccaagctg ctgtcataaa actgttcaaa tgcccatgga ggttaaaata accatacata
     8641 cgaatataaa aatagctgaa tattaaatgt gctaatgaaa gaaaagcaat actaagtgta
     8701 aaaaaaagat gtttcttctg catgattgtt ccagtccctt actaagatag atagtcttta
     8761 ttataccacg tgtgagggga gaaataaaaa gccgtttttt agtagatatg aaacaaaaca
     8821 aaaacccgca tttattcaac ctattcatcc ttttaaagga tagattgaac gtagatagaa
     8881 gtcgtttatc cagtcttttt cattttgaaa aaaagctaac agcatgcttt ttttatggta
     8941 tactagcaag gaaacgaatt cgcatcatgc gataaaggag gaagagcccc gtggcagaaa
     9001 aggaaacttt ttatatcact acgccaatct attatccaag cggcaaactt catatcggca
     9061 actcgtatac tacgattgca tgtgacgcaa ttgctcgtta taaacggttg atgggctttg
     9121 atgtgtttta tctcactgga gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag
     9181 aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc ggctgatgtc aagaaattat
     9241 ggaaaacgct tgatatcagt tatgataaat tcattcgcac gacagatgat taccataaag
     9301 cagctgttca aaaaatattt gatcgtttat tagaacaggg agatatttac ttaggtgaat
     9361 atgaaggctg gtactctgtt tctgacgaag aattctttac agaaacacaa cttgcagaag
     9421 tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag
     9481 aattggtaaa agaagaatca tacttcttcc gcatgagcaa atacgcagac cgtctattgg
     9541 aatactatga agaacatccc gaattcatcc agccagaatc tcgtaaaaac gaaatgatca
     9601 acaactttat caaaccagga ttagaggatc ttgcagtttc acgaacaact tttacttggg
     9661 gagtaccagt caagagtgat ccgaaacatg tggtgtatgt atggatcgat gcattatcca
     9721 attatattac tgcactagga tatggctccg aagatgaaag tctattcgaa aaatactggc
     9781 ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa
     9841 tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt atttgggcat ggctggctat
     9901 tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg
     9961 tggaacgtta tggtttagat gcactccgtt attatttgct tcgtgctgtt ccgtttgggt
    10021 cagatggtgt ctttacgcca gaagattttg tttcccgtgt caattatgat ctggcaaatg
    10081 atctagggaa cttattgaat cgaacgattg ctatgatcaa caaatattgt gatggactcg
    10141 ttcctgacta tgcctcactt gtcactccat ttgatagtga actgtctacg acggctgcta
    10201 acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga attcaataca gcattggctg
    10261 aaatctgggt gctgatttct cgtgcaaaca aatacattga tgaaacagaa ccatgggtat
    10321 tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt catgatccat ttagctgaat
    10381 ctttgcgtat cgcagcgata ttacttcagc caatcatgac agaaacgcca gagaagatct
    10441 tcaatcaact aggattggac tctgaaacaa tgaatcttga aggattacac tttggcgaat
    10501 ttccttcagg aacaaaagta gttgcaaaag gtacaccaat cttcccacgt ttagatatgg
    10561 aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg tactcaaaca aacgaagata
    10621 cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat
    10681 ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt catcaactgt caaaaagtag
    10741 aaggggcaga taagttgctg caattccgac tagatgctgg agacagccaa gaccgtcaaa
    10801 tcttgtcagg aatcgctgaa ttttatcctg atccaagcga attgatcggg aaaaaagtcg
    10861 tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc
    10921 tttctgcaga agcaccagat ggctctttac aagtaatcga agcaccaaaa tccatgccaa
    10981 atggctcaga aatcgcatag aaattcaaag ggggtatgac actagttgtg tcataccccc
    11041 tttttccgaa atttgagaag caattttcgt aaattccttc ttattacttg aagccaatca
    11101 tataatacag aaacagagaa ctatccaaaa taaatcctag aaaaagaagt acgagcttcg
    11161 ctgatttttg ttaatttaga ataatactaa taggaatcgt aaaaacagaa aaaaagcata
    11221 atgataaagg gtcgggcaga ttttaattga ttcatctggt actctttttt attgtcctta
    11281 cattcataaa gtttgaatgc aatgacttta ttacatctat atttatatct aacagtagcg
    11341 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
    11401 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
    11461 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
    11521 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
    11581 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
    11641 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
    11701 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
    11761 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
    11821 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
    11881 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
    11941 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
    12001 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
    12061 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
    12121 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
    12181 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
    12241 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
    12301 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
    12361 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
    12421 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
    12481 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
    12541 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
    12601 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
    12661 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
    12721 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
    12781 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
    12841 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
    12901 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
    12961 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
    13021 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
    13081 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
    13141 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
    13201 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
    13261 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
    13321 tggctattca gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa
    13381 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
    13441 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
    13501 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
    13561 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
    13621 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
    13681 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
    13741 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
    13801 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
    13861 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
    13921 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
    13981 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
    14041 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
    14101 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
    14161 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
    14221 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
    14281 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
    14341 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
    14401 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
    14461 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
    14521 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
    14581 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
    14641 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
    14701 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
    14761 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
    14821 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
    14881 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
    14941 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
    15001 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
    15061 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
    15121 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
    15181 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
    15241 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
    15301 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
    15361 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
    15421 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
    15481 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
    15541 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
    15601 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
    15661 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
    15721 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
    15781 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
    15841 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
    15901 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
    15961 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
    16021 cgtttgtttg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
    16081 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
    16141 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
    16201 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
    16261 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
    16321 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
    16381 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
    16441 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
    16501 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
    16561 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
    16621 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
    16681 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
    16741 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
    16801 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
    16861 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
    16921 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
    16981 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
    17041 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
    17101 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
    17161 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
    17221 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
    17281 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
    17341 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
    17401 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
    17461 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
    17521 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
    17581 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
    17641 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
    17701 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
    17761 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
    17821 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
    17881 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
    17941 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
    18001 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
    18061 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
    18121 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
    18181 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
    18241 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
    18301 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
    18361 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
    18421 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
    18481 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
    18541 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
    18601 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
    18661 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
    18721 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
    18781 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
    18841 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
    18901 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
    18961 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
    19021 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
    19081 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
    19141 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
    19201 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
    19261 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
    19321 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
    19381 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
    19441 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
    19501 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
    19561 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
    19621 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
    19681 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
    19741 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
    19801 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
    19861 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
    19921 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
    19981 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
    20041 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
    20101 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
    20161 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
    20221 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
    20281 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
    20341 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
    20401 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
    20461 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
    20521 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
    20581 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
    20641 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
    20701 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
    20761 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
    20821 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
    20881 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
    20941 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
    21001 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
    21061 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
    21121 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
    21181 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
    21241 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
    21301 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
    21361 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
    21421 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
    21481 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
    21541 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
    21601 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
    21661 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
    21721 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
    21781 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
    21841 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
    21901 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
    21961 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
    22021 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
    22081 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
    22141 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
    22201 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
    22261 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
    22321 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
    22381 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
    22441 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
    22501 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
    22561 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
    22621 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
    22681 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
    22741 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
    22801 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
    22861 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
    22921 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
    22981 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
    23041 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
    23101 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
    23161 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
    23221 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
    23281 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag
    23341 agaacaagcc ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga
    23401 ctattt
//
LOCUS       DAZKSR010000044        23358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000044 DAZKSR010000000
VERSION     DAZKSR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.044"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACG8XG_001522"
     CDS             138..995
                     /locus_tag="ACG8XG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0773445.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACG8XG_001523"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACG8XG_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0773446.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACG8XG_001524"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACG8XG_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0773447.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACG8XG_001525"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACG8XG_001525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0773448.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACG8XG_001526"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACG8XG_001526"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0773449.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACG8XG_001527"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACG8XG_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0773450.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACG8XG_001528"
     CDS             5437..5736
                     /locus_tag="ACG8XG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773451.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACG8XG_001529"
     CDS             5777..6469
                     /locus_tag="ACG8XG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0773452.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACG8XG_001530"
     CDS             6466..7233
                     /locus_tag="ACG8XG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0773453.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACG8XG_001531"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACG8XG_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0773454.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACG8XG_001532"
     CDS             9198..9518
                     /locus_tag="ACG8XG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0773455.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACG8XG_001533"
     CDS             9518..9868
                     /locus_tag="ACG8XG_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0773456.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACG8XG_001534"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACG8XG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0773457.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACG8XG_001535"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACG8XG_001535"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0773458.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACG8XG_001536"
     CDS             11862..12887
                     /locus_tag="ACG8XG_001536"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0773459.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACG8XG_001537"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACG8XG_001537"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0773460.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACG8XG_001538"
     CDS             13965..14405
                     /locus_tag="ACG8XG_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0773461.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACG8XG_001539"
     CDS             14408..15010
                     /locus_tag="ACG8XG_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0773462.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACG8XG_001540"
     CDS             complement(15078..16184)
                     /locus_tag="ACG8XG_001540"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0773463.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG8XG_001541"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG8XG_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0773464.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACG8XG_001542"
     CDS             complement(17731..20076)
                     /locus_tag="ACG8XG_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0773465.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACG8XG_001543"
     CDS             20355..21257
                     /locus_tag="ACG8XG_001543"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0773466.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACG8XG_001544"
     CDS             21470..23245
                     /locus_tag="ACG8XG_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0773467.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7443 a         4142 c         4926 g         6847 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggacta
//
LOCUS       DAZKSR010000045        23035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000045 DAZKSR010000000
VERSION     DAZKSR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.045"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2564)
                     /locus_tag="ACG8XG_001545"
     CDS             complement(<1..2564)
                     /locus_tag="ACG8XG_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0773468.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
     gene            complement(2786..3670)
                     /locus_tag="ACG8XG_001546"
     CDS             complement(2786..3670)
                     /locus_tag="ACG8XG_001546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0773469.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3765..4202)
                     /locus_tag="ACG8XG_001547"
     CDS             complement(3765..4202)
                     /locus_tag="ACG8XG_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773470.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4419..5330)
                     /locus_tag="ACG8XG_001548"
     CDS             complement(4419..5330)
                     /locus_tag="ACG8XG_001548"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0773471.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5707..6345)
                     /locus_tag="ACG8XG_001549"
     CDS             complement(5707..6345)
                     /locus_tag="ACG8XG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773472.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6349..8331)
                     /locus_tag="ACG8XG_001550"
                     /pseudo
     CDS             complement(6349..8331)
                     /locus_tag="ACG8XG_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            8511..9176
                     /locus_tag="ACG8XG_001551"
     CDS             8511..9176
                     /locus_tag="ACG8XG_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773473.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9573..11606)
                     /gene="brxL"
                     /locus_tag="ACG8XG_001552"
     CDS             complement(9573..11606)
                     /gene="brxL"
                     /locus_tag="ACG8XG_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0773474.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11724..14246)
                     /gene="pglZ"
                     /locus_tag="ACG8XG_001553"
     CDS             complement(11724..14246)
                     /gene="pglZ"
                     /locus_tag="ACG8XG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0773475.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14362..17994)
                     /gene="pglX"
                     /locus_tag="ACG8XG_001554"
     CDS             complement(14362..17994)
                     /gene="pglX"
                     /locus_tag="ACG8XG_001554"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0773476.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18058..21729)
                     /gene="brxC"
                     /locus_tag="ACG8XG_001555"
     CDS             complement(18058..21729)
                     /gene="brxC"
                     /locus_tag="ACG8XG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0773477.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21754..22329)
                     /locus_tag="ACG8XG_001556"
     CDS             complement(21754..22329)
                     /locus_tag="ACG8XG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0773478.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22329..22928)
                     /locus_tag="ACG8XG_001557"
     CDS             complement(22329..22928)
                     /locus_tag="ACG8XG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0773479.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4531 c         3344 g         8167 t
ORIGIN      
        1 gggatagaaa gaacaaattt acctgtatca tcaatagttg tttcggacaa aacagcttga
       61 ttttttttca atgaaaccaa agctcctgct tcgcctgtac ctgtgattga gcgagaagct
      121 gcattgatca atgtggtact taatttagca ggctcaggag gaatcacatc gactgtcgtt
      181 ttggcagagt ccagttctgt cacttcttgg ccgcctctta cttttttagc agcaactatt
      241 tgcacttgat cccccgttgt tagtctttct tcgttgggta cttgaacctc aaacaaaccg
      301 ccttgttcgc cttctcccca cggattatat cctgtccctt cattttcatc tgtttgtcct
      361 tgtgtgagag cagtaatttc tacaggtgat ttcgtcgtat ctgcataagt aatacgtaaa
      421 gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca
      481 acgacatgcc catagatttt tttatctgca ttcgttggta ctcttaagga ttcaatcatc
      541 ggaagctggt tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca
      601 aattcatttg ttaaacctgc actagggctt gatacaaaat tacctaagtc ggttccattg
      661 atcgataagt ctgtcatatc agaaaaataa tccggatctt tttcaaagtt atcgaacgtt
      721 actcctgcct ttcgccataa agcaatttct gaatgagcga tcttcaaaga agaatcgcca
      781 tttccttgaa atacccagcc acctatgcca ttagtctcgc ttccgatccg tttgttttgg
      841 aaatcgaaat aggtaggaga ttctaagtct acattcaaca catccccact gatgatgcca
      901 gtcttatctt ccccagtttt cccgaccatg actaaataag ttttttcgcc agccataaag
      961 ttcaaatcaa tatctccagt tgtatctacg gtagctccgg aatctgcgcg tatatcgata
     1021 tttgattttt ctccagtaac cgtaaaatca gctgaccctg gactttctag aaaaccagaa
     1081 gcgtcataaa attggatcgc ttggtttcct tgattgagtc tgttcacata agatttgcca
     1141 tcccctgcat ttcttacctt caatgagcct ccattagtta ctgttagttt ttgcgtaccg
     1201 ttctcaaaac gaactgcgct agaacgacca ctttgcttat caatatcgat gactgatccg
     1261 gctccttcta cgatcaaaga aagtcctttc cctacaaaac gcatcccatt gtttccaggt
     1321 tggtcgccat ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga
     1381 ctagaatctc cttgcaggcg aaatccgcca gtagtcgctg tattgattgt gattttccca
     1441 tcattcgaca cagcgactct tgatcctgag cctctcattt ggataattcc gcgattgttg
     1501 tcgttggtaa tattgatctt tgtatttacc gaaaattcac ttcctgagcc actgacttta
     1561 aatgtgacat tcccgggtag cgtatcgaca gcctctcctt catcgctgac gccatctgcc
     1621 cgatcatagt caagagagac ctttgaacca ctcaagatat ggagtcctgg tgctgttttt
     1681 ccactgcccg tctcatcacc tgaatctaga aaaccataga attttcctag atatggtcca
     1741 taaagctgac taccattgat tacatttagc tgatagcttc tacaaaaata agctttaccc
     1801 ggagcggcat tcatcccttc tttcactggt tggacatcca atacaccgga aacaccatct
     1861 aatgtgacag ttgcacgagg ggcgtaaatc aggcctaatt tagaaccatc atataagtca
     1921 agtgtcccgg taaattttat ttctcccgta aaatgatatc tgtttcccca aaaatcggac
     1981 atataagcaa tgataatggc atttccatcg atatttggta tcaacccacc tggatgagct
     2041 gccgttcctt taaaattctg aaactcgatc gactgaggtg tctcattgat atagatgtga
     2101 tgtgtgccga aattatgagc aagtccgcca aaatcgataa cgatatctcg cttgatctcc
     2161 aattccctgt ctaaagtcaa aggctgagtt aaacgaatct tagctactga ttcgtctgcg
     2221 agaagctgcg tcagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacaact
     2281 ctaacctgta cctcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt
     2341 gtcttatttt cttcttgcct tgtattagaa gtaggcgcct cactttggga ttctgtagtt
     2401 tcagaagtaa ttgattcttc tgaaaaagta gtcttgtcaa aactttgatt ctctgattgc
     2461 gttgattcgc ttgttttctc tacatcggca aaaacggatg tcaccatcaa gagagggaag
     2521 acaaacaaaa tcgacgcaat cagctttttt acatattgat tcattctcca ccatcctctc
     2581 tgacgttttg acaattttat tttaacgttt taatttattt gtaattgtac tttcatattt
     2641 aaaatatgta tttttatggc aaatttttct gttaggtgaa aatttctttt ttgtacaaaa
     2701 aaaggctgtg acaaaagtct tgtcacagtc ccttattccg aacaaccgat gtcctcctga
     2761 caactcttcg gtcacaacct ccattttaac aataaacctg taaatgtgag ggtaacactt
     2821 tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct
     2881 catcagctaa tgacacaact aattgctcac aagtaagata ggctagattt ttcgtagatt
     2941 catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt
     3001 ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa
     3061 aaccgcctac tgagttagtt agtcctatca gcaagatact gctctcaatt tcttccttct
     3121 ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg
     3181 acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg
     3241 cttcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa
     3301 tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg
     3361 gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg
     3421 gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca
     3481 tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc
     3541 ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg
     3601 ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa
     3661 cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag
     3721 aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga
     3781 taaaataccc atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa
     3841 aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa
     3901 aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc
     3961 agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacgacct
     4021 tgaataataa atgcaacaat ccccgctata attagtgtgc ctaataccaa aacggaaaat
     4081 tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga
     4141 aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca aaaccactcc
     4201 atttatctac ttctacgtgc attttttaat atcgttttct ttatttaaga ttatacagaa
     4261 aaatatctag ggtggctgga ttctaagtaa aaaaaatttt taaaataaaa agaactaaat
     4321 caagtaataa ttaaactact gttgatttag ttctaataaa aagatttaga tgaacagttt
     4381 tattgattta aatagttcat attttttatt tataccgctt atttactact tgctgtacta
     4441 gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat ttttacatta
     4501 gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact taattttgtg
     4561 tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc tttgttttgg
     4621 tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt ggtagaagtg
     4681 atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac ttctgccgga
     4741 accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt cgtagtatca
     4801 gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc tttcttcaca
     4861 tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc tggatggaaa
     4921 gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt tagaccttct
     4981 tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta acctgcagat
     5041 ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata ttgggaatcg
     5101 atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata aacgatcaac
     5161 tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac ttcttgttcg
     5221 ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt acagcctgac
     5281 aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat ttatcaattc
     5341 tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg aaaatcatca
     5401 agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta cacaatccag
     5461 aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat actacttctt
     5521 ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa ttcttagaac
     5581 aaatgttatc gattttgttt ttcttcttcc gctgttggga tattcaaatc agacactttt
     5641 atgttcgatt ttccagacat ttttttgttg tttttattag aaaaagtttg ttttttcatt
     5701 tgttttctaa agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac
     5761 cacgataact aacgtacctt gggaagcctg ttgcgataag atttcaacga cttgctcctt
     5821 atttcgttta tctaatgatc cagttggttc atcggctaaa atgattttgc tattcttcaa
     5881 taagactctc gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt
     5941 acttagcttc ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc
     6001 tgaaacattt aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata
     6061 ttcaatcaac ccaaaatctt gaaaaagaaa agagagggca tttcttttta tcaagcgctt
     6121 ctctcttcca gttaaatgtt ttgtttcttg attaaataaa taaaattgcc ctgaactgaa
     6181 ttgatcattc aatcctaata tattcaataa agtacttttc cctgctccgc tctctccaac
     6241 tacagtcaca aaatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt
     6301 tcgaatgatt tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt
     6361 catcagcaat aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa
     6421 atcaatgaag ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa
     6481 aaggaagatt acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata
     6541 cgtttcaaag agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac
     6601 aaaatatatg cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa
     6661 tatctgtatc aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg
     6721 agtatacgga aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa
     6781 taacctttct ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct
     6841 gagaatattt ctttcccaat acgtactgtt tttcaatgtc agtatttcaa caaccggata
     6901 atgttcgatc caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa
     6961 tagaaatttc ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc
     7021 attttttaaa tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt
     7081 ttcggttatt tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt
     7141 cagatttatt tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac
     7201 attgactaat atacttccgt ttttattcaa agtttcatat aattcctgat cggctgctga
     7261 tgcaaaacga ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa
     7321 tacatgatag tcctcaccag cgatttcttt tggtgtttca tgggaaacag ctaactgtga
     7381 agaagaaaaa acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa
     7441 caatccaaat gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat
     7501 tttttcaact aataaaagag aaatcaaaga gataccctat attccgctga acaagaaaac
     7561 gtacatctgg aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat
     7621 gattcctgca attatgaatg taataatgat tttctttttc cccaataaac accacaatac
     7681 ttgatgtaat gaaaagccat tattcaacaa taaactgatt tctttattca tcaataagaa
     7741 aaaggatgat aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt
     7801 atatacgtct gtacttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat
     7861 attagtggca taatcagtgt gaaatgcttg attgaatcgt tccacaaata gtaaaatgaa
     7921 ccgttcatag gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat
     7981 tttgatcgat tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc
     8041 gtgagaagat ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt
     8101 gaagttccaa gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag
     8161 ctcagtttct gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc
     8221 aataaaatgt tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt
     8281 tgtccgatag agtacctgtt tatttataat aaccaaggca aaaccactca tgataccggt
     8341 cactatgatt gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt
     8401 aaatgattct ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaaatgta
     8461 taggtcatgt agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacatcg
     8521 gagaaaaaat acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa
     8581 aaatcggtgt ttccgataaa acaatctcta gttgggaaaa ttctcgtaca atgccagacc
     8641 ttgaaatgct gtcactttta catcagactt taggtctgcc gattgatttt ccttctattg
     8701 aatcagctac cgaatcaata gaaccaacat tgccggatga acctcttgtt atcaagaaat
     8761 cttattcatc aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct
     8821 tttgtttggt ctatcttttt cttatcaatt atcaatataa aaatgaaacg gttgatcgat
     8881 tcaatccttt tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaaa
     8941 attccatcga acattgggta tgtaatgata tctttgttca tggtacatgg ctgtcttttc
     9001 acgctggaga ggtagaaaaa ggtaagaatc atgctgtaat caaacacaaa ggaaaacacg
     9061 tcatgaacgt tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca
     9121 tccaagacta tgacgatgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag
     9181 gagatgaacg tagagataga aaaaatcaat gcacatgatc ttatcaaaaa aaaacagggg
     9241 tacaaattaa ttgaaaaagc tttgagatga aagttcctaa ataaaaaagt tcagaatgat
     9301 aggtaaaaaa atgactaact attctagtat taggctattt gtattattct tctgttattt
     9361 ttatatcaaa aaatccagaa ataactaaat ataggtattt ctggcttata gtataatatt
     9421 tacgccacag cccccaatga ttctttttgg aatcatgatc aaggacggac atacacaaaa
     9481 atattttgcc cattcgatcc gaatagatta acgactaatc tagtaagtaa tatagattgg
     9541 tacgtagtca aaccagcaaa tggaaaagtt gtctattcca ccccaagggc tttaaagaca
     9601 gcatcttccg gcgtttgata aaaaatcaaa ttaaaggtac ccattaattc tgggggtaca
     9661 gttccaatat cagcagcgct tatcataggt acaagtactt ttttcgctcc ggcatctttt
     9721 gctacttgta aacagttagc taaattatct accttaatca tagaaccacc aattgtaata
     9781 tcccctaaaa taactaaact tggtaataca ggtttattta aggaaactga gcataaagct
     9841 attatagttg ataacgataa tgtttttgtc ataccaatgc cattcatatc ttgaacatga
     9901 acgacaaaat cttttgtcgt caaactaata gactgactaa cacttccata attagctctt
     9961 aaatatcggt atgccgaatc catcgcttct cttgctccag catctgaatt gatcccagta
    10021 cgttcaaact ttccatttcc cgaagtcatt tgcgtttcca atttataggt acctaattgc
    10081 ccagttttgc cataagatac cgtgtacaaa tgccctggct tccctaaccc ttctggaatc
    10141 aaagttcctc caccttgttc aggaacagaa acatactctt ctcgctcttc ttctaaatca
    10201 atgtatgaaa aattgacatc ataaaattcc attccaccaa ttttctttaa ttgttctttt
    10261 acacgacgac gcatttcaag agcaaatctt agtatttctt cgacatcact tttttcaaat
    10321 ctaccatcag gatacaacag tttcatcatc ccagatacaa tccgacgaac tgcaatcgta
    10381 tcccgctgat ttaattgaga accaagtttg aaatatttat caaaactatc gccaaaacta
    10441 acttttcgaa gctcacgcat gacttcagaa aaataatctg taatgaagcc atagtcattt
    10501 gtaaaaaaat ctggtcgata tttaggaatt tcccaacctg gtaaataaca atgcatccga
    10561 tccaaaaagg ctgaatccgt ccccataacc tccggaaatg gctcaaataa attcgaagtt
    10621 ttcaatatta catcaacgct ttgattaata tttcctacaa aagccattga tgctgaagca
    10681 ttcttttctt ctttgcctct agcaaaagat cctgaagcca tgtaatcttt catgatttga
    10741 acgccatcat tatccttaaa tttaatccca gcaacttcgt caaaagcaac acagtcccac
    10801 atacctacaa gtcccattgt acgactagcc atattataga aaaggtttgc tactgttgtt
    10861 tgtccacctg aaacaaggat tgagtttggt gaaatttctt tatatacatg cgatttacct
    10921 gtaccacgtg gtcctagttc acagagatta aaattatttt caattaatgg aataagtctt
    10981 gctaaatgaa gccattttac ccttttatta aattgtgtag gttccatacc aatggaccgt
    11041 agaagtaagt caatccattc atttgtagta aactccttgc gagccatctt gtaagcctca
    11101 atgtctaaac caggcatttg gattggatca accttttttg caataaaagg gctattccaa
    11161 cgatcattct catcatacca atattccatc gaaacaatac accagatccc cccacctaat
    11221 aatcgatcaa attttttcgg atactcttca ctaatttgaa cattttttaa tcccaggtta
    11281 gagaattctg cttcataaca atctttcttt tcatttaaat gtacagttac tttatcaata
    11341 actgtataac cacccaattc acgaattttt gcttttactt tttctgcttc atcaggacgc
    11401 acatagtttc gagcaagaat atttttaaca ttttccacac ctttttcaac caatgtttca
    11461 tccaatgacg aagcatacat accaagtaaa tattccaata cataaacagg tacattcgca
    11521 ccttctttaa ttttctttgt taaatctttt ctgacaatct ttccgggaaa gaattcagat
    11581 aactttaaaa tttcctgttg ttccactaga attcctccat gtgttcagtc attataatag
    11641 caattaaagt atatcatact taaccagtag gcttgataat acaaaagaaa aagtatccac
    11701 aaaaaattgt ggatacctcg tttttatata tcaaaatcaa atgattctgc aaaaggatta
    11761 tctataatga attttattcg ctcggtcaaa tcattttttt gattttttat cactaaataa
    11821 taagtatctt caactgtata tcgacgattt acaaattcaa aaacaaattt agtaaaccgc
    11881 tgatttgcat cttctgtttt tgaatccgca acatagattt cttcatttga gatacgttca
    11941 ccatattcat cttcaaaata aagccagaaa gatgcggggt cataaacatc actaattgca
    12001 tttttttgat agaattctaa cgtggttgat aatcctgtaa ttctgcggtt gctagtcatt
    12061 aattcaattg ctactggttc ctttgcattg ctacctttgg ctaccgtaat ttctagaaca
    12121 gggactagca tttcttgaat agatgaccct ccatgaacgt agctttgtcc cccgccagct
    12181 ttcttaaaaa tacttgaagt aatcgggaca caaatgtttc tagtgtcatc atttcctaaa
    12241 gaatctccca aagagtaatt tttcgtacca attatttcgt attcttcttc actaagaata
    12301 aaacgacgtt ctacacgatc tttttcattt ctagatggat tttcaatctt atctacttct
    12361 tcaacgtggc ttcttttata aataaatcca tgatcagccg taactataaa acgatagata
    12421 ctgccatcat tatgcaagcg attaataagt cgggaaattt cgcttatcgc taaatcacag
    12481 gcctcaaaaa ctcgattttc attcgagtga tcacctgttc tatccacttg atcgtgataa
    12541 atataaatga ctgatttgcc attaaatact tctcgtacct cagccttatt catcttaacc
    12601 acatcatcat aatttaaagc tacagcattt gcatctcgtt gctggagaat cgcatttcga
    12661 ttggataaac cattcgtttt tttcccatca atcagtactt ctgttccttc attgtaagtt
    12721 agttgattgt ttggtaataa ggcggcttta ccgaattcag taactgaagg taatacagaa
    12781 agaaagctag ccatgtttgt tgaatacttc ttttctctaa ctaacttacg ttctaaattc
    12841 atccctaact catatcgaaa agcatctgaa ataataacta cagttttaac agagcgtttc
    12901 attacagctt gattataaaa ttttcgaatg gatggacgat tcttaaattc aaaagtttga
    12961 ttccaaattt gtcctgattg atcaagaaac ttcgaataat ttgcttcaac taatcgatac
    13021 aattggccat atttttgctt ttcttctata tgatcactat tccaaataaa tttccgataa
    13081 tgcgtatcaa cccaatacat ttccttctca tattgagtta gcaaattatc catgttcatc
    13141 ggcaagcaca atctgttttt atccacaaga taataagcat gtttcaataa ttgatactca
    13201 taaatataat ccgatgcgaa atgcattttt tcgcgctgct ttaaactatc aaaaattgtt
    13261 tgtcctgcaa ttttcgttgt agtgtcagaa gcctttaatc gttctgcata aaacattaat
    13321 atttttgcat gaatctcaga aaatacgtct gcttcaatta gatcatcaat ttctatactg
    13381 tctaataatc catcaccatc aatagcctga tatacttttt tagatagctg ttcaaagctt
    13441 tccatgtaac gtgtatcatt cataaactga tccatgaaag taataatgtt tgctttcaaa
    13501 tgtagtattt caaatccttt gaacttcttc ggcatctctt gatcaatttg ataataaaaa
    13561 tagttcaaat aaagacacat cgtcagcttt tccaatgttg gctggctatc cgtaaagcca
    13621 aatttcattt gaataaattc ccaaaattta gctgttaact gatacttatc aagttccctt
    13681 aaaggattct ccctactttc tgtaaagctt tttagaacta cacgcagtaa atcatttaat
    13741 cgaatacttt tcgatttcaa caaaacagcc attatcatta attcaatcgt ttctgattta
    13801 ttaattgtca tatgcgtact aaattcacca aaacttctca tgcgatcctt tgcattaaag
    13861 aaattacgat actgtttaac tgtctgatga aatctaagtg gaatagccaa ttcattcatg
    13921 ataatcgcta agcggtctgc actaaattcc tctgaataat tcaatacact caacaaatga
    13981 ttatcttcat ctttatcatc ccaacgagga aatggcgcat agattaaata gttagtttcg
    14041 ggatcttcat attccaataa acgcttagtt ttaaattgtg cgtccttttc taatcgatag
    14101 atttttgcat tttgaatgac caatgagtca atttcttcaa gaaaatcctg atttgaatca
    14161 aaccaaaaaa tgattttcct acatccataa ttaggaaaac gttgatttaa tttttcttca
    14221 atttgttgcg tatttatatc tgtcatcaat ctcacctaat cgttctcttt ctatacccct
    14281 tatattgaca taatttggat aatttcgcac gcaatttaag aataaaaaag aaagttgtta
    14341 gaaaacaact ttcttagata actatttttt tggttctttg accccttttg caaaaatttg
    14401 gaatttctcg ccggttttag gatcacgctg caacttatca taattgacaa ttacaccatc
    14461 atctaagtct aattcaattc gttttaatgc taagtgaccc aacttctcgt catactcacg
    14521 aacttctttt aattgcttct gaatctttac aatttctttt gtcattgcat ttctttcttt
    14581 tgggttatca cttgttgaaa tttgttgctg acgcaattcg attcgattgt catacgcttt
    14641 ttgtagttcg tgtaggtatt cagtacgtac ttttcctaat tgatctggtg tatagcgatg
    14701 caagtacatt aacgctttaa agccattttg ttttcctgag tccaattgcc aataaattgg
    14761 tcgattggag tatgttttta aatggtcttt gtaaaaatct tgtacgaagt aacgacgaat
    14821 aatttctcga cttgtccctt tgccacctaa agtgtcagcg ataaactgga gattttcttc
    14881 aagggtttct ttaccaaaag taatctcaac gaaatcaatg aaacgattga caatgtcaat
    14941 ttggctatcc ttgaaatact catcatcggt gatgagtaaa acattatcag tatcaggttt
    15001 aaatttttga tagcgggaag gatcccattc gccgccagcg aagatgagac catcttcgtc
    15061 tagggagtaa cgaccgaaga tacaacctat tgaataagaa ataaaatcct taatttccct
    15121 cgccttatca ggtaaccttg cagagatata ttcatcttga acctcttttg ttaattcttt
    15181 ttcaagagaa taatgattaa tgaatatgtc gttcagttcc tcttcattcg attttagagt
    15241 attaaattga ttaaccaact gcctacttag acagttaaat gcctctgaga gatctttttt
    15301 aaacatataa cttaacaaaa tgtttcttct aaactcgtat gacgtttcaa gagcattata
    15361 gtcttttcta gctatatcga tattttcatc cactttttct cttatttttt ttaattttct
    15421 ctcagatgta actttaagaa aaggtagttt tcccattggt ccttcatggt aatccaaggt
    15481 gggagaaata aaagttaaaa agtgattaac taatttagag tttacatagc caaatatttc
    15541 ttcaaagtca gttatatcct ttgagaacag aacagatcct ttagtttcaa aaattgcctg
    15601 attctgcaat ctaaaagata cttgtccaga tgaaagtgtc gaccaagtaa ttcccttttt
    15661 aaaatagtaa ttaagatttt gtggtctaga ttttaacttc ccattttcat taacataatt
    15721 ttttatttca tctccatcat tctcccagtt aaccacgtat tcattgtttc cataccattt
    15781 tctaaaacga ccacctttag ttgtaggaaa ccattttttt ctagactttt tagcgtcctc
    15841 taatgtttta gcatcaaact ttattttgtt aaattctact tcaaaccaat accgcaaaaa
    15901 tttattgtta tttccggttg ccaacccttg ttttgcatct gcaacatcgt tagttaattt
    15961 tccttgagca aaatcagtga ttacgttatc gcttgcccag taagcaatcg gcattcctgg
    16021 gatttttttg aagttgtctt gattagtact ataaaaataa tcgcattcac catgctgatg
    16081 aattgcttct aacgttttaa ttttttgaac ttccattcca cctttaaaat ctgataattt
    16141 tatatagctt ccatcttgtt catttgaatt ttttatcgta aacgtacaaa ttggaacggt
    16201 tgcttcttca aaagctgaat actccatttg aatcaaacta gaaatgtttc tttcattaat
    16261 taaaaatgag cgtaactttt catagctaga aatgaacatc caaacaaatg gcgtcataaa
    16321 ggaagcatat ccattatcaa tcagcatttg gctattcaat ttaataaaca cagaaaatag
    16381 atcttttttg acatcagaat agttatcatt tacatatttt tttaatttct tatccatttt
    16441 attaagataa ggcggattag tgactaccgc tacatattta tcaactagca tttcagccac
    16501 gtcaagaatg tgcaagtatt tttcttgtat ttccttcatc ccataaagaa tatccatatt
    16561 cgtaaattcg ttgaattttg atatatactc tcttactttt cctaagtatt ctttagttat
    16621 tgcctgatta tttttatcga ctaacttaat gattgatcca agttctttcc cattttcaaa
    16681 ttgatcaaaa attaactctt tgtattcatt ccagcgttca ctatacttat cattgttcaa
    16741 ttcactaata cgctcttgat actcgtctgt cactttagga gaatcaataa actctcttaa
    16801 attcggcgat agactttttt tcaaaaaaag acgatcgtat tgacgccctt tcatcataat
    16861 tgcaaaataa gacaattgat acgctctttt atcgatttca agaccatata aattgtattc
    16921 aacgattgca cgtgcagcat cacgctcaga atatcctaac gtaagataaa tttccatcaa
    16981 tacatcgaag gcgtaaacta aaatatgccc agaccccatt gcgttatcga taatttttag
    17041 ttcttctggt tttaaattac ttacagatgt tgcttgttca aaagcatatt tatctggaat
    17101 taagtatttc aacttatttc ttaattcact attgggataa tgatctaacc aatactttcc
    17161 taaactattg tcaaccatat aacgaacgac ccaatctgtg gtaaatagtt gagttgctgc
    17221 cggaatatct tgttttttga caatcccttt ataaatattc accacttgat tatgtggctc
    17281 actattatag tactgataca accacccaat aatctctacc tgtccaccag cttgaacata
    17341 gaaatcatcc tctggaatac ttaaaagatc agcgattaca ccatcttttt taatataaga
    17401 agctgtaaac aaaagttctg tataatcttc tacttcttca aatagctcag gtaagtaatc
    17461 attcaattga ttacagattt ttataaaaat caattgatac agcttgtcca ttgcttctgc
    17521 tgaattatct gttttccaag ttctgacttg ttgctgttca ttactagata attcttcaaa
    17581 gatatcggca tcataaatat ctgtaataat atctggctca attttcccac ttatttcact
    17641 agataataca cgatacgtgt caggtaaata atcatttacc tccataaacc gaatggcaat
    17701 caatcggtta aaccacgtat aagcaacctc ttcgattaac tcttgaaaag cttctgaata
    17761 agaagtgaca gaagcacgtt gttttagttc tgcaatcaat ttgtttcttt gcgctatttc
    17821 ttttccttga attcttattc ctgatttttc gatttcttgt aaattgctat caattttctt
    17881 cacatcttca atttttgatt catcaattcc taaacgtttt gctttagtca ttacttcatc
    17941 aattaatttt ctacgggcat tgaccgcgaa attctttatc gctttcttat ccatttttct
    18001 ttcccttcta tatgtaacct ttgaaaagac aggcaccttt ttatgcctgt cttttcacta
    18061 gaattgaatc gtgatttctg tattttcttg aagttgttcc ttcagttttt gattaatttc
    18121 agctgttaac tcatcaatat ctgctgctga cttaatagtt ttaacatgaa cgttaagtgt
    18181 tctcattggt actgttttct gttgaatagt aggttttact atcgctattt cttcagcttt
    18241 tgctgcagag tctatttcat ctttttcagt ttcacgaatc aagtttgctg cttcaatttg
    18301 tttacgtgct tgttcttctt gacgtctgcg gttttcttca ttatcgaaac gttgattaaa
    18361 gatcaaatac tgttctgcta cgtttaatga taagaaaaca agatcatcta tagtattcgc
    18421 ttgttcaatt tttgctttca gttcttgaaa ttcttggtca actattggta caaattgatc
    18481 cttaaaatct tttccttcta ataatttaaa tgttgcttgt ttttcttccg ttaatttatt
    18541 taatatttcc tgactttcca actctaacag ttgattatat tgttcaataa attcttgaat
    18601 caactcattt agtttcatca tatctgaata aggatcagcc tttttcaaaa tggttttcat
    18661 ctcatgaatc aattgatcaa tttcttggct agaagtttga cgccgacttt tttcgattaa
    18721 tctaatttta tcttgtgcat tctcccagat ttttttctga gtactatttt gcttgtggaa
    18781 atttttaatt ttatcatatg tttcagccca gtcgaataat tcatcgcgat attttttgat
    18841 tgtataaaaa aagtcttcaa catctgacgt ttgaacaatt acttcaagat atttttttat
    18901 ttgctcatat tcataagcac tagggtaggt ataagtgttt tgtaaatcaa aataagagcg
    18961 taaattgtct aattgacctt ttaaatattt cttgaaattt tccattgtat gttcattgtt
    19021 attttcatca acaatattac gactttgaaa cagttctcta ccgatttcgt caaccaattt
    19081 ttttgctttt ggcgaaatca ccgcgcttgg ttcaattttt atcttttcac tttctcgtcg
    19141 tttttgaata atcattacaa tactatttgg atcatccatc cgtgaaattt gtacattgtt
    19201 ataaagtaaa tcaattttac cagacatgaa aagtttagcg atgcaccatt ccacatcaag
    19261 ttctgtgaat ccatacggtt gttttgtttc aaaacgatca cgaattgttt tcatcgtaat
    19321 cgttaggtga tttctctttt gatcattgat aaaattgaaa atttcctgaa ctgcttgctc
    19381 atttgatgga tcttcaataa tttcattttt gctttctttc actaaatata agatatcttc
    19441 tttttcttta ggcgcatcaa tttggtttaa tttatggtat acttcttcag ccattttctg
    19501 aagtgctgat tggaattttg ctttaaagtc ggagcctgtc agttgaattt ctgcaccgtt
    19561 atagaaaaat tttgcagatt tcaacagttc atttaattga tcgttgatac ttgcacttag
    19621 ttcctgtaat tcaccagatt tttccgaacg aatcgcgtca aagttcttta ctacactcga
    19681 ataagtcgtt gttaaataaa gattcattct agcatacatt gctatatcat ctttaaattc
    19741 tttatctgat ggcaattcta cgaataataa gttacgggaa ctcttaagcg caagttctgc
    19801 ctcttgacct gctaaatcag agtatggtga aataaaatta actgtcattt cagcatttcc
    19861 agcttgcttg tatggacgtt gatctaacac ttgtgcataa gtaaacgtat accggttttt
    19921 aaatttattt ccacctgaca tgcctaattt ttcataggca aaactatttc ggtctagaac
    19981 gccgtcaaaa attcgtttag atagttctac gatcaaatca ctatctgtaa tattttgatt
    20041 tttaatctca cgagtaattt cctgttcttc atcagtttgg aaaatataac tatctaagtt
    20101 cttccgcaca aagttttcct taattaaaag gtccaatgct tcttctactc gttttttagt
    20161 ttccaggcgg tcttcgttga tatttcttat catcaagctc gtaatatttt caacatttgg
    20221 tttgatggct tgatcaacat atttaatcat gaataaaatt cgtaagacat ttacgttaaa
    20281 gttatctata tcagttggtc tttgaatatg agaattattt gtagcttgtg taatcactcg
    20341 gctaattgct gtatcaatcc aacgttctaa cgagtaataa aactgatcta aagaaaccaa
    20401 actggttgta tcattttcct tatttcgttc agcggcttct ttaaacattg ctagtaatga
    20461 acgttcacca gacgataagt ttgatccttg atagctgtga ttacgaatct cggttagaat
    20521 ttgccccaat aaattgaatt gatagggaat aaatggataa acttctgcgt aattttcagc
    20581 atcttggaag agctttaatt caggactatc aacaaagagt aatttatttt tcaattctgt
    20641 ttgaacagtt tcaaattgta aggctaaaga atcattggca aactcttttt tcttcaaaat
    20701 acgttcttta attacttcat cggcatttgc tgaagataaa ctcacagttg tggcaaaacg
    20761 atccttgatc ttagaaaaat cattatcaga attgttatca aagtttgtta cgtgatcaat
    20821 cgcttcctga cttgtcacta atacccaagc cttaccttca caaattcgac ccaaatcttc
    20881 cacaacggtt tgtaactgca aagtcaactc acgattaccg ccgatatatt gtccgacctc
    20941 atcgactaga aaggcaatat gatggtctgc ttctttggaa tcaagatagc ttttaactag
    21001 atttgcaaag tcatcaatgc ttattaaata atttcctgat gctttttcta aaaaattatc
    21061 aatagcggct tcactcataa aatcgatttt aagtgccgct tctttgatat catcttggat
    21121 ccaatcaaac tcatggcgct cttcttgcca ttttgcccca ttaatctctt caaaagcagc
    21181 tttgaatgca tcaaactttt cttctttaat taaccgacgt tcaaaatctg ctagatgtgg
    21241 aatcgtgctt aatccttgca tttcgttaaa tacctttaaa aaaacattca cgattccgtt
    21301 tttgtctttt ttaccacctg catcactttt tgaatcaata ttaaacagaa taacatctgt
    21361 atttatttgt gagcaccgtt ccatcgttgc caaagtcagt ggatctttga ttttcccatc
    21421 ttctttgaag taatcaatgg ctttcttgtc atttatttct cgatttgcga gcaagtaaga
    21481 aataattttt aagaaatgag atttacctga accaaagaac ccctgaatcc aaacacccat
    21541 tttatctgtt ggtgtattga tactccgttg gtaggcatca aaaattgttt taaagtgttt
    21601 ttgtagttct ttcgtaacca cgtattcttc taattcttgt tgaacatttt catcctttgc
    21661 ttgaccgacc ttaataactc cttgaatatt tcggtcaatt ggttttgcaa aaatatcttt
    21721 aattagcatt taatctccta ccttccatcc caactaaatt ctaaatgctc gataataatt
    21781 atcatcttta ttggtattaa gaattgtcaa acttaattca tcatattccc ctggatacat
    21841 catcacaact ggtacgcgat caaatacctg atgcatttta tttaaaatag agtgcgctct
    21901 aattaatgga tataattcgc caattcctgt caggaacaca actgattttt ctggtgtatg
    21961 ggcatccaca tattcaaaaa atacattttc ttcctcaggt aaatccattt tcaataaacg
    22021 accaatgctt tccactaatt tttgtaaacc acgtcgttct tccatcttaa tcgctttttc
    22081 agtgaaaccg cgtttttcta gatactctaa cataatcaca tagagattat aaactttcac
    22141 ttcataatta tcaccaacag atgttccaga tatatcatta atataatctc ttacaattaa
    22201 ctcgtctttc ggatcgtagt caaagatata atagcctact tcattaccta aaccacgatt
    22261 caataaaaaa tcttcttttt gaattacttc attcaacttt tccaaatgct cttgaatcga
    22321 taatgacatt ataaaattcc tcccagtgct agtacaatat ctttacgatt cttgtcaact
    22381 aaccattgaa tcaatcgttt atctaataat atcggagtca ttcgtccata ttctttttcc
    22441 cctctgacta atcctgtttc aattagattc gtttgataag cggcaataag ccgtttaatc
    22501 gtataatctt tccattttga aatatcttct gaacttctcc gcttctctgt aaaaaaagaa
    22561 cggtaatcaa ctggtgttaa ttgcaaattt cctttacgta gattttcagc atatacctca
    22621 gtcataaatt cattaaaaag atcatcaatc ataaggatgg aaatcaaatt cactaacttt
    22681 tgattatcta tatttaactg attgaaaagt tgcaatccag cttcatccag ttgactaact
    22741 ctccttttca cgttctgata gatttctttt tgacggttac tgctcactgc aaaaaaaata
    22801 ttttcatgtt catttttctc tttaatttct atatctgtat atccttctag tcgtaattga
    22861 atatataact tgaactctga aaaccaaaag cgtttagaaa ctagaccagc agaatatttt
    22921 ttattcaata gattgcctcc taatcatcta taattcaata agtaaacaag tgttttttgc
    22981 tcatcgttag cattcatttt ttagtatcta ctatacaata catatttatt atagt
//
LOCUS       DAZKSR010000046        22921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000046 DAZKSR010000000
VERSION     DAZKSR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.046"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..444)
                     /locus_tag="ACG8XG_001558"
     CDS             complement(160..444)
                     /locus_tag="ACG8XG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773480.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="ACG8XG_001559"
     CDS             complement(462..1352)
                     /locus_tag="ACG8XG_001559"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0773481.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="ACG8XG_001560"
     CDS             complement(1372..1590)
                     /locus_tag="ACG8XG_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0773482.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="ACG8XG_001561"
     CDS             complement(1560..3260)
                     /locus_tag="ACG8XG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0773483.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="ACG8XG_001562"
     CDS             3527..4300
                     /locus_tag="ACG8XG_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773484.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="ACG8XG_001563"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="ACG8XG_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0773485.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="ACG8XG_001564"
     CDS             complement(5124..6095)
                     /locus_tag="ACG8XG_001564"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0773486.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="ACG8XG_001565"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="ACG8XG_001565"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0773487.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="ACG8XG_001566"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="ACG8XG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0773488.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="ACG8XG_001567"
     CDS             complement(9050..9874)
                     /locus_tag="ACG8XG_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0773489.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="ACG8XG_001568"
     CDS             complement(9847..10527)
                     /locus_tag="ACG8XG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773490.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="ACG8XG_001569"
     CDS             complement(10550..11488)
                     /locus_tag="ACG8XG_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0773491.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /gene="ispE"
                     /locus_tag="ACG8XG_001570"
     CDS             complement(11733..12584)
                     /gene="ispE"
                     /locus_tag="ACG8XG_001570"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0773492.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="ACG8XG_001571"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="ACG8XG_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0773493.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHLTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="ACG8XG_001572"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="ACG8XG_001572"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0773494.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="ACG8XG_001573"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="ACG8XG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0773495.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="ACG8XG_001574"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="ACG8XG_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0773496.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="ACG8XG_001575"
     CDS             complement(19462..20703)
                     /locus_tag="ACG8XG_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0773497.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="ACG8XG_001576"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="ACG8XG_001576"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0773498.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22653)
                     /locus_tag="ACG8XG_001577"
     CDS             complement(21730..22653)
                     /locus_tag="ACG8XG_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0773499.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         6520 a         4990 c         3970 g         7441 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaggtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 t
//
LOCUS       DAZKSR010000047        22735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000047 DAZKSR010000000
VERSION     DAZKSR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.047"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1958)
                     /locus_tag="ACG8XG_001578"
     CDS             complement(27..1958)
                     /locus_tag="ACG8XG_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0773500.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1976..2890)
                     /gene="pfkB"
                     /locus_tag="ACG8XG_001579"
     CDS             complement(1976..2890)
                     /gene="pfkB"
                     /locus_tag="ACG8XG_001579"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0773501.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2887..3639)
                     /locus_tag="ACG8XG_001580"
     CDS             complement(2887..3639)
                     /locus_tag="ACG8XG_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0773502.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3799..4008)
                     /locus_tag="ACG8XG_001581"
     CDS             complement(3799..4008)
                     /locus_tag="ACG8XG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773503.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4019..5346)
                     /locus_tag="ACG8XG_001582"
                     /pseudo
     CDS             complement(4019..5346)
                     /locus_tag="ACG8XG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5482..5637
                     /locus_tag="ACG8XG_001583"
     CDS             5482..5637
                     /locus_tag="ACG8XG_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0773504.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5711..6298)
                     /gene="yihA"
                     /locus_tag="ACG8XG_001584"
     CDS             complement(5711..6298)
                     /gene="yihA"
                     /locus_tag="ACG8XG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0773505.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6381..7631)
                     /gene="clpX"
                     /locus_tag="ACG8XG_001585"
     CDS             complement(6381..7631)
                     /gene="clpX"
                     /locus_tag="ACG8XG_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0773506.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7878..9167)
                     /gene="tig"
                     /locus_tag="ACG8XG_001586"
     CDS             complement(7878..9167)
                     /gene="tig"
                     /locus_tag="ACG8XG_001586"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0773507.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9321..10253)
                     /locus_tag="ACG8XG_001587"
     CDS             complement(9321..10253)
                     /locus_tag="ACG8XG_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773508.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10410..11069)
                     /locus_tag="ACG8XG_001588"
     CDS             complement(10410..11069)
                     /locus_tag="ACG8XG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0773509.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11072..12097)
                     /gene="sppA"
                     /locus_tag="ACG8XG_001589"
     CDS             complement(11072..12097)
                     /gene="sppA"
                     /locus_tag="ACG8XG_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0773510.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12110..12400)
                     /locus_tag="ACG8XG_001590"
     CDS             complement(12110..12400)
                     /locus_tag="ACG8XG_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773511.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12809..14614
                     /gene="glmS"
                     /locus_tag="ACG8XG_001591"
     CDS             12809..14614
                     /gene="glmS"
                     /locus_tag="ACG8XG_001591"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0773512.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14686..16041)
                     /gene="glmM"
                     /locus_tag="ACG8XG_001592"
     CDS             complement(14686..16041)
                     /gene="glmM"
                     /locus_tag="ACG8XG_001592"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0773513.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16065..17234)
                     /locus_tag="ACG8XG_001593"
     CDS             complement(16065..17234)
                     /locus_tag="ACG8XG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0773514.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17231..18118)
                     /gene="cdaA"
                     /locus_tag="ACG8XG_001594"
     CDS             complement(17231..18118)
                     /gene="cdaA"
                     /locus_tag="ACG8XG_001594"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0773515.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18307..21999)
                     /gene="nifJ"
                     /locus_tag="ACG8XG_001595"
     CDS             complement(18307..21999)
                     /gene="nifJ"
                     /locus_tag="ACG8XG_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0773516.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22198..22719
                     /locus_tag="ACG8XG_001596"
     CDS             22198..22719
                     /locus_tag="ACG8XG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0773517.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6333 a         4939 c         4044 g         7419 t
ORIGIN      
        1 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
       61 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
      121 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
      181 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
      241 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
      301 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
      361 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
      421 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
      481 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
      541 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
      601 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
      661 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
      721 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
      781 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
      841 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
      901 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
      961 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
     1021 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
     1081 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
     1141 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
     1201 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
     1261 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
     1321 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
     1381 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
     1441 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
     1501 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
     1561 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
     1621 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
     1681 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
     1741 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
     1801 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
     1861 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
     1921 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
     1981 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
     2041 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
     2101 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
     2161 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
     2221 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
     2281 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
     2341 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
     2401 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
     2461 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
     2521 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
     2581 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
     2641 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
     2701 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
     2761 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
     2821 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
     2881 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
     2941 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
     3001 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
     3061 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
     3121 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
     3181 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
     3241 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
     3301 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
     3361 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
     3421 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
     3481 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
     3541 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
     3601 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
     3661 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
     3721 tataaatcag tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
     3781 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
     3841 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
     3901 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
     3961 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
     4021 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
     4081 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
     4141 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
     4201 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
     4261 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
     4321 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
     4381 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
     4441 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg
     4501 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
     4561 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
     4621 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
     4681 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
     4741 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
     4801 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
     4861 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
     4921 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
     4981 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
     5041 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
     5101 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
     5161 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
     5221 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
     5281 gtttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
     5341 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
     5401 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
     5461 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
     5521 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
     5581 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
     5641 aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg gtaagtcttt
     5701 tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct tcttttccct
     5761 gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc aacgcttttt
     5821 tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc ttagtggcaa
     5881 caacaattac agggatatca taatacttca aaaattgata catctggata tcttcttgag
     5941 aaggcttatg acgcaaatct actaaagaaa cgaccgcacg tagctggtct ctagtcgtta
     6001 aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact tttgcatacc
     6061 catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct
     6121 gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc aatgtgttga
     6181 taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc aattgtgtat
     6241 ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg actttcatgg
     6301 tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc caaaatgact
     6361 tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata gattactgtt
     6421 ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat gttttcattt
     6481 gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg aagtcctcga
     6541 gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc aggttcgaat
     6601 tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag cgcatttttc
     6661 ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc tgccataaca
     6721 ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc aggaatgata
     6781 tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc
     6841 ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc acctacgatg
     6901 aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt acgtccgccc
     6961 tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg ttggacacct
     7021 tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat tttatcgatc
     7081 tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc agattgaagc
     7141 aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt caaacttgtc
     7201 gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc taaaaatgtt
     7261 ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc aacatcatct
     7321 gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc aacagctaac
     7381 gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag aatttctagt
     7441 ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt
     7501 tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc tacgatcttt
     7561 ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt gctacttgga
     7621 ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac tatcaaagac
     7681 tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt taacaaagga
     7741 tcaactgacc tatacctttg cattttttac tcttaacaaa aaataaggaa ctgtgacaaa
     7801 atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt cttcagaaat
     7861 gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat caattcgatt
     7921 gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg tttgatttgt
     7981 tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat ttcttcttct
     8041 gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt tcttgtgcgc
     8101 atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc agttaattga
     8161 tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc gtccattgaa
     8221 cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc gttttcaaca
     8281 gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt tgtttctact
     8341 aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc atcaacatct
     8401 tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg gattgtcact
     8461 ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg gaaagttaca
     8521 tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa acctggaatg
     8581 aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg
     8641 ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc tgcagcatct
     8701 tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca
     8761 gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg tcctaatttt
     8821 acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt ttccatgctt
     8881 tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac agcagcttca
     8941 taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc accgtacata
     9001 cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt caagcttttt
     9061 ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat
     9121 tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta attccctccg
     9181 agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa atagtacact
     9241 aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca taacattttt
     9301 atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat tttttctctt
     9361 ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt tcctgtacac
     9421 gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc atagacagga
     9481 taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag cagcgcatct
     9541 tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg ctgctgcggc
     9601 tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa atagaaaaca
     9661 ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg
     9721 acctctggcg atacaataag tttactcgct gtttggataa actcatgata aggaagctgt
     9781 tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag ttcctccgat
     9841 ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc ttctgcaaat
     9901 tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact tgctatcaat
     9961 tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa ttcagcaaac
    10021 tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc ttcttttttc
    10081 tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct ttctttcaac
    10141 tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt
    10201 tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt cacgcagtgt
    10261 cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa agcatgacaa
    10321 ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt tagaaggtgc
    10381 tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt gaacgctttg
    10441 atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg ttgctttttc
    10501 tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag aatgaaccgg
    10561 caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc aaagctgatg
    10621 actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt cgtcaataaa
    10681 ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag ataagaatct
    10741 gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt aatagctccg
    10801 atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc atagaaatac
    10861 ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt tttccattta
    10921 cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc
    10981 ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc tttttcttgt
    11041 tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa tacattgctt
    11101 ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta gtctcagaag
    11161 gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca aacccagatg
    11221 gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt cgtatagctt
    11281 ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt gcttggacac
    11341 catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta ttccgtccct
    11401 cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga agcaccgctt
    11461 cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct ccgcttttaa
    11521 ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat cctgacatga
    11581 tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct gcttgagcag
    11641 aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt gggatatcat
    11701 gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag acaccgccac
    11761 caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca atcgccttca
    11821 gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct gaagaccctc
    11881 ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg tctccctctt
    11941 ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat ccctcaattt
    12001 gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca aataatcctg
    12061 cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc taatacaagt
    12121 tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct gctttttctt
    12181 cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta aactcacgag
    12241 cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag ccagtatcta
    12301 aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac aattgttttt
    12361 ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct cctctatccg
    12421 attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc attatttttt
    12481 gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt ttattctttt
    12541 tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa
    12601 aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
    12661 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
    12721 aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
    12781 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
    12841 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
    12901 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
    12961 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
    13021 acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
    13081 tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc
    13141 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
    13201 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
    13261 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
    13321 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
    13381 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
    13441 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
    13501 ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat
    13561 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
    13621 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
    13681 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt
    13741 ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac
    13801 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
    13861 cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
    13921 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
    13981 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
    14041 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
    14101 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
    14161 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc
    14221 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
    14281 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
    14341 accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
    14401 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
    14461 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
    14521 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
    14581 acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac
    14641 atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat
    14701 ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg
    14761 cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc
    14821 ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat
    14881 cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga ttgttacctc
    14941 tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc
    15001 tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc
    15061 gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg
    15121 tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat
    15181 gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca
    15241 gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg
    15301 atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc
    15361 taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt ctggatttgt
    15421 gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt
    15481 tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg
    15541 tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc
    15601 ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag
    15661 ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg
    15721 tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag
    15781 ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa
    15841 aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac
    15901 taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg
    15961 tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc
    16021 tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa
    16081 tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc
    16141 gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct
    16201 gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc
    16261 gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg
    16321 ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg
    16381 atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta
    16441 gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca
    16501 gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca
    16561 gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc
    16621 agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca
    16681 tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt
    16741 ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc
    16801 aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat
    16861 ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacttcatgt
    16921 gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt
    16981 gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca
    17041 tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac ccccgtaact
    17101 gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg
    17161 aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc
    17221 tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca
    17281 cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag attgccaaat
    17341 attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg acgtccccgg
    17401 tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg acagctgccc
    17461 ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga agataggcgc
    17521 ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag
    17581 gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca atccctgtct
    17641 caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg atttttcgct
    17701 tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt
    17761 cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt
    17821 ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc
    17881 aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta
    17941 cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa
    18001 gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac
    18061 cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa
    18121 aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag
    18181 gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa ttgcaagtat
    18241 gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt
    18301 tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc
    18361 tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg ttttttcttt
    18421 tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct tgattttttc
    18481 taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt ggacagtctt
    18541 ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa agcgaacttg
    18601 tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca aagtcattgg
    18661 gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat aaccagaaga
    18721 aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc catgtgtgat
    18781 acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt cttcaaatgc
    18841 cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa catttccata
    18901 tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag ccgaaaattt
    18961 agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg aatagacttc
    19021 gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat cgatcccgcc
    19081 aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt tttttacgaa
    19141 tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt cgctttcgat
    19201 cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca tgtgtactaa
    19261 ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt ctttgattga
    19321 acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac caaattcagc
    19381 attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat tggtcgtata
    19441 aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat tcgcgatcat
    19501 cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc cacagcctga
    19561 gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga cagtattctt
    19621 tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat tcacagcttg
    19681 ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg gacaagcctc
    19741 tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt attttacgcc
    19801 gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca tttcctcttc
    19861 atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt cattacacat
    19921 cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct tttccataga
    19981 agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg taccaacaga
    20041 gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat aaccagtgtt
    20101 ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga tcgcgacttg
    20161 atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga tagccattga
    20221 tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct catcaaaagg
    20281 aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc ggcgtcccaa
    20341 accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt tattttcagc
    20401 caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc tccaaagagt
    20461 attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc tatgcaggta
    20521 agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgcag aacgtatcgg
    20581 cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg aatcataata
    20641 aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag ctgatttatt
    20701 tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg tttgcggttc
    20761 tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat ccacgatccc
    20821 tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa aaactgctga
    20881 gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa taacagccgg
    20941 gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt ctccacctgc
    21001 ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag gcaacttttg
    21061 cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac ctgttttccg
    21121 tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg atcccataga
    21181 aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat aagaagttcc
    21241 tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt tatcataata
    21301 ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg taccactaac
    21361 agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct cctgattgat
    21421 cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt gactcgtacg
    21481 gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa gatgggcaac
    21541 aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa atcctgtttg
    21601 tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg tcgtcactgc
    21661 ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt acatattcgg
    21721 tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac cagttttcaa
    21781 tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta cttttactgg
    21841 ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt cagccatggt
    21901 cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag aaatatatgc
    21961 ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc cttgttcata
    22021 ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc ttttatttat
    22081 aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca aacagtctaa
    22141 atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg
    22201 gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca
    22261 tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta ttgccgacaa
    22321 tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga
    22381 gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt gatgcaccgg
    22441 tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat attcgatact
    22501 caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta
    22561 aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa
    22621 gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga tgaatctatt
    22681 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtg
//
LOCUS       DAZKSR010000048        22608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000048 DAZKSR010000000
VERSION     DAZKSR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.048"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG8XG_001597"
     CDS             complement(<1..613)
                     /locus_tag="ACG8XG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0773518.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            886..3357
                     /locus_tag="ACG8XG_001598"
     CDS             886..3357
                     /locus_tag="ACG8XG_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0773519.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3460..5196)
                     /locus_tag="ACG8XG_001599"
     CDS             complement(3460..5196)
                     /locus_tag="ACG8XG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0773520.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5193..5897)
                     /locus_tag="ACG8XG_001600"
     CDS             complement(5193..5897)
                     /locus_tag="ACG8XG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0773521.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6028..6531
                     /locus_tag="ACG8XG_001601"
     CDS             6028..6531
                     /locus_tag="ACG8XG_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0773522.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6491..6829
                     /locus_tag="ACG8XG_001602"
     CDS             6491..6829
                     /locus_tag="ACG8XG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0773523.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6850..8268
                     /gene="ffh"
                     /locus_tag="ACG8XG_001603"
     CDS             6850..8268
                     /gene="ffh"
                     /locus_tag="ACG8XG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0773524.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8380..8958
                     /locus_tag="ACG8XG_001604"
     CDS             8380..8958
                     /locus_tag="ACG8XG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0773525.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8962..9483
                     /locus_tag="ACG8XG_001605"
     CDS             8962..9483
                     /locus_tag="ACG8XG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0773526.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9562..10116)
                     /locus_tag="ACG8XG_001606"
     CDS             complement(9562..10116)
                     /locus_tag="ACG8XG_001606"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0773527.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10369..10644
                     /gene="rpsP"
                     /locus_tag="ACG8XG_001607"
     CDS             10369..10644
                     /gene="rpsP"
                     /locus_tag="ACG8XG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0773528.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10656..10907
                     /locus_tag="ACG8XG_001608"
     CDS             10656..10907
                     /locus_tag="ACG8XG_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0773529.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11032..11823
                     /locus_tag="ACG8XG_001609"
     CDS             11032..11823
                     /locus_tag="ACG8XG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773530.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11993..12514
                     /gene="rimM"
                     /locus_tag="ACG8XG_001610"
     CDS             11993..12514
                     /gene="rimM"
                     /locus_tag="ACG8XG_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0773531.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12504..13265
                     /gene="trmD"
                     /locus_tag="ACG8XG_001611"
     CDS             12504..13265
                     /gene="trmD"
                     /locus_tag="ACG8XG_001611"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0773532.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13401..13748
                     /gene="rplS"
                     /locus_tag="ACG8XG_001612"
     CDS             13401..13748
                     /gene="rplS"
                     /locus_tag="ACG8XG_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0773533.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14121..14783
                     /locus_tag="ACG8XG_001613"
     CDS             14121..14783
                     /locus_tag="ACG8XG_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773534.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14862..18089
                     /gene="fss3"
                     /locus_tag="ACG8XG_001614"
     CDS             14862..18089
                     /gene="fss3"
                     /locus_tag="ACG8XG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0773535.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18203..18517
                     /locus_tag="ACG8XG_001615"
     CDS             18203..18517
                     /locus_tag="ACG8XG_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0773536.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18530..18904
                     /locus_tag="ACG8XG_001616"
     CDS             18530..18904
                     /locus_tag="ACG8XG_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0773537.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18914..19258
                     /locus_tag="ACG8XG_001617"
     CDS             18914..19258
                     /locus_tag="ACG8XG_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0773538.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19329..20681
                     /locus_tag="ACG8XG_001618"
     CDS             19329..20681
                     /locus_tag="ACG8XG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0773539.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20741..21883
                     /locus_tag="ACG8XG_001619"
     CDS             20741..21883
                     /locus_tag="ACG8XG_001619"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0773540.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21908..22162
                     /locus_tag="ACG8XG_001620"
     CDS             21908..22162
                     /locus_tag="ACG8XG_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773541.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22418..>22608
                     /locus_tag="ACG8XG_001621"
     CDS             22418..>22608
                     /locus_tag="ACG8XG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773542.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNP"
BASE COUNT         7506 a         4052 c         4821 g         6229 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga
      781 aaggtgtttg caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga
      841 gaagtttgga aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg
      901 ataaatcgaa gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt
      961 tttccatcgt ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt
     1021 gcagcaaata attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac
     1081 atatttacgt atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa
     1141 tcctataaga acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt
     1201 caggaaattc gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc
     1261 aatgtcatac gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac
     1321 attgttattt tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa
     1381 ctgcaagtta atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt
     1441 gatccattaa tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa
     1501 gttggagcag attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga
     1561 gaacagtcgg agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt
     1621 attcctatcg gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa
     1681 gaaagtgtcg ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg
     1741 ctgacgacga ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta
     1801 cacaatatga aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca
     1861 ggaacgatca cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa
     1921 ataatcagtc agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca
     1981 ggagacaatg caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc
     2041 tatcaccatg ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac
     2101 aaggtgtatg ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg
     2161 gaagagtttg tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat
     2221 gggattcttg aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt
     2281 gctaatccta tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta
     2341 gccatcaaag tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca
     2401 gggatttcac atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa
     2461 gcagctgtcg aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc
     2521 gttcaattga taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt
     2581 ctagccatga aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg
     2641 caggcctcac aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca
     2701 gaaggacggc gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat
     2761 attttctcct ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa
     2821 ccatcacaaa tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct
     2881 ctagaagaaa acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct
     2941 gtgccaggtg gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact
     3001 ttgaatctaa ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg
     3061 ttcctcgttc tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt
     3121 ttctgtgcta gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc
     3181 ggtatctcag ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt
     3241 tttaggcatt tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa
     3301 aagccgttta gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga
     3361 aaagtatctc aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg
     3421 agtcatccaa ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac
     3481 ggtgaaagtc gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg
     3541 cacgtagtct tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc
     3601 cttgtctacg cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat
     3661 accaaagtct tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc
     3721 gctgtcgctt tttgaatatt ggacagcatt ttcaattaga tttttgataa ttggataaag
     3781 gatatctgtt ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt
     3841 cttttcgatg attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat
     3901 tgtctggatc tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag
     3961 tttatctaac cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg
     4021 gtcatctttt gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt
     4081 ttttaattcg tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac
     4141 tttcgttaag tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta
     4201 gcgcaaactc aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag
     4261 cggatgctca ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc
     4321 tgtgtaccgc tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt
     4381 aagcagtagt aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt
     4441 cagtaacgta tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg
     4501 ttcgctcaac gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat
     4561 tacttccgcc tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa
     4621 tggctggttt ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga
     4681 cagaaagaag aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt
     4741 cggttctgcg atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac
     4801 atataaaaga tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc
     4861 attcaatacg gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt
     4921 tgtgtcaaat aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg
     4981 gacgaattct tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg
     5041 ccggatcagc agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg
     5101 ttgaaaataa tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag
     5161 aaagaagaga cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag
     5221 ccaaatcctc taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct
     5281 cgcaaatgac taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa
     5341 atgcggtcta gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca
     5401 agtaattcaa attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa
     5461 ttggtcaaat cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt
     5521 gattgcttac gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga
     5581 ggactaaatg gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc
     5641 acttggtcat ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc
     5701 cgctgagtga tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca
     5761 aatgattgtt ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg
     5821 tagccttctt tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca
     5881 actagtattt ttttcattca aatctccttt aaataggttt cattctgttt atattttagc
     5941 atagaatcag tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact
     6001 ttgctgaata gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg
     6061 gcctgtcgct atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact
     6121 ggagaagctg tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc
     6181 gctgaaatca ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg
     6241 acaagcggcg aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa
     6301 ttgcaggaaa aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc
     6361 cattcgattt atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact
     6421 gcttatttta ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga
     6481 ggtgagcaga atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc
     6541 gaccttattg acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc
     6601 attaggtgag atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa
     6661 gcggacaagc aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat
     6721 tgttcgagaa caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga
     6781 taaaaaacta cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat
     6841 gaagaaaata tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata
     6901 agaaaaaaag gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt
     6961 gccttactag aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc
     7021 gaacgtgcgg taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa
     7081 atcgttgatg aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca
     7141 ccaaaaatac caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt
     7201 actggtaaat tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca
     7261 ggggacgtat atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac
     7321 gttcctgtat ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg
     7381 gagttagcaa aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat
     7441 atcgatgaaa cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag
     7501 attttgcttg ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc
     7561 aaccagcaat taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc
     7621 ggggcagcac tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt
     7681 gaaaaattga ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt
     7741 atgggggata tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg
     7801 gaagaacttg cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag
     7861 cttgatcaag tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg
     7921 aatcaagtcc ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa
     7981 gcgatggttt attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc
     8041 cgtcgtcgcc gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc
     8101 aaacaattca aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc
     8161 ccaggtatgg accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg
     8221 aatcgaatga tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac
     8281 agcttcggta cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc
     8341 tttactaata caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa
     8401 caaacagaaa gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac
     8461 caaaagctgt tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg
     8521 attttaaata aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc
     8581 gcaaattatg gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac
     8641 cgaagaaaaa tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa
     8701 atcggcagtt tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct
     8761 tatcattcac aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat
     8821 ctaaagaaaa gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct
     8881 gtcgggatca tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc
     8941 ggcagaaagg gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat
     9001 ggctatttgg caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag
     9061 caaactactt atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact
     9121 tatcaggaag caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc
     9181 gttttttcca cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct
     9241 gttgcttttc taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc
     9301 ggtcgattga tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt
     9361 acccctgtat tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt
     9421 tttgaaatgc tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag
     9481 tagaagtaaa taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct
     9541 tttttattac cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc
     9601 taaaatcgtt tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag
     9661 attcgcaacg tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac
     9721 aagtgattgg cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt
     9781 aacgactaaa cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc
     9841 taaagcattt ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac
     9901 accgtctagt cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg
     9961 agtttcgaga tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta
    10021 cccttcagga agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct
    10081 taaactacca ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat
    10141 ctataataat cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag
    10201 ctacatagaa gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc
    10261 taaaaaaaat cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa
    10321 ctgttacact acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa
    10381 atccgtttaa aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat
    10441 tcacgttctc ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa
    10501 gaccctgcag aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca
    10561 caaccttctg acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac
    10621 gaagctaaat tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct
    10681 taacaatcgt tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt
    10741 cgaaagactt ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg
    10801 gaaaacaagg gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg
    10861 caccgaaaaa agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt
    10921 actcgaattt tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg
    10981 gcaaaacaag caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag
    11041 aaaatgttat ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa
    11101 tcagacaata cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt
    11161 agtaccaaaa cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag
    11221 actgctttag accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca
    11281 gtctcttcag gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta
    11341 tattacagaa cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct
    11401 attgcaacct atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg
    11461 cagccatttg agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg
    11521 tatcagcaag gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt
    11581 cgaatccgtg ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta
    11641 gcttatttgg aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat
    11701 atgggtgata cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat
    11761 acgatcactc atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg
    11821 tgaaatcagc gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa
    11881 aatagcacgc cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg
    11941 cctcttcaca catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga
    12001 atacttgaat gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat
    12061 ctcaacaacg gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca
    12121 agacggaaaa gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga
    12181 cttattgagt tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat
    12241 attaaaagtg tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga
    12301 gatcatcggt ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt
    12361 gtcaccaggt gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct
    12421 tccatatatc gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga
    12481 aattccagaa ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt
    12541 ttgaaggtcc tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata
    12601 tcaatatctc taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc
    12661 catacggcgg tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag
    12721 ccattgaaaa agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta
    12781 aacgattcga tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt
    12841 gtggtcatta tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct
    12901 taggagatta tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag
    12961 ttcggctttt gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa
    13021 ccggtttatt ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac
    13081 cagaagtatt gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac
    13141 taagaaggac ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa
    13201 tgacgaaatt tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca
    13261 agtgactttt tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg
    13321 agtgcaaaag cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt
    13381 ggaataagga gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac
    13441 gttctgacat cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg
    13501 aaggtactcg tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg
    13561 gaatcagcga aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat
    13621 tcccattgca tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc
    13681 gtgcaaaatt gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatca
    13741 gaagataaac acggaaaact tccttggaaa tctgaaattt gtgaaaagtc cttgtatatc
    13801 aagggctttt tcttttttct ctatcacagt tttaagccat ttttggctgt tttggaccat
    13861 ttttcaatca tattccctta aaaattgtcc tagattgtcc gacattgact atcagtgtcg
    13921 tcaaaatagg gcaaaattta gtagtcagct tgacgacaaa tataatacga aaaataccaa
    13981 aacatttacc gaatttagca acttttgaac ccaagtttct caaagggtca gctattagca
    14041 aaaaagacaa aacatgtact tttctactaa aaagttgatg tgataggctt ctatctaaat
    14101 aactattagg agtttttatc gtgaagcaaa atgatggacg tatcatttgg aaaaatcaaa
    14161 gtgagttaaa actgatttta acaattaatg aattcatcga aaaacacgga attacatcta
    14221 gccgtcaata ccagaaaaag ctatcagaaa atccaaattc tgcaccaagt atgtggttca
    14281 ttaacaaaaa atatggatca tgggagaatt tattgattag tataggacga gaaaatactg
    14341 ggtatggaaa atgggctaga atgtcagaac aagaattatt ggaaatagta gaagccttta
    14401 ttaagtgcga gaagataacc tctcaacgaa tgtacgaaca aaaatcagta gggaagaata
    14461 ttccttcact tagtaccatt aaaaagatgt taggagatat acgtccactt tttaaagaaa
    14521 aaaatgatgg atctcgattt actgatttcg aactgctgtt agaactaaaa aatgaaataa
    14581 tccgattaaa actacaggat gatttatcaa tgacaaagtt tcgaaaacta gttcaatccc
    14641 caaaactacc atcagttgat acaattatga aaagaacgaa caaaaattgg gaggaattga
    14701 tggcagagat tggatttgat tatcgaagaa tcaaaattta taagcagaga aacaacctat
    14761 ccaagacaaa gaaaactaaa tagtatcact ctaaatcaga cgttcaaaat taaaatttgg
    14821 acgtcttttt tattacccaa aaacgaaagg agatttattt tatgaaaaag ccaagattta
    14881 agaattggcg tttgctcgca acactgagtc tattatgcca aacaattggt ggatcgcttg
    14941 gcccaacgat tgcctttgct gatgaaatta ctcatccaca aacggtaact gtggaattgg
    15001 atttggctca ccagtatgct gtcgaaggta catttagcga tggtcgtccc atgtcagaag
    15061 ttaccgttcc gcactatgca gtttacaatg gtgtgaagca agatattttc tgtattgagc
    15121 caggggtgcc gatttacaat gagttcactc ctggttatga gaaaaatcca ttgcctgata
    15181 tgtcggagaa agcgaaatta gtttccgttt tatggaagga agctggtacg gatgtggata
    15241 cacatattgt ggcccagaag atgatttggc aagaagttaa tggctacacg cttcactcaa
    15301 ttaaacgttc tgatggtagt gcagtaaata tcgcagccat tgagtcaaaa atcaatcaag
    15361 ccattgcgga ttatcaaaag aaaccaagct tccataactc cacagtgaaa accgtgttag
    15421 gtcaatcgac tactgtgaca gatacgaacg gcttgaattt atcggagttt gatgaagttg
    15481 tcgagaatat cgcaaatatt gattatcgtg tgaacggcaa tcaactagtg attactccaa
    15541 atgccaattc aaaagagagt ggtgaattaa ccctaaagaa gtcagctaat acaggaacac
    15601 cggtagctta taaaatggct ggtcaacaaa ctttgatggc gggggcaatt gataagccca
    15661 atacctacac aatcaaaatt gatgtggaaa ctgagggttc tttgaagatt aaaaaagtcg
    15721 ataaagaaac aggaaatgtt gtaccgggaa ccgttttcca tttagacttt ggaaaaacat
    15781 tacctgcaaa agacgtgacc accgataaag aaggcattgc gacattggat gagattcctc
    15841 atggcgcgaa agtaaccatt actgaaaaat cagtgccagc tccttatacg attgacacta
    15901 caccaatcac cgcaaccatt aaatctggtg agactatttc tgtgacttca aaaaatactc
    15961 gcgaaaaagg tcaaatcatt ttgaataaaa tgggggtgga aacaggaact gatctttgga
    16021 acgacaaata ctcattagcc ggaaatacgt ttgccattcg caaagacagt ccaaccggtg
    16081 aaattgtcca agaaatgacc acggatgaaa aaggtcatgc ggaaacacca aaagagattg
    16141 ccaatgcgtt ggaattggga acctattatg tgaccgaaac caaagctagt aatggctttg
    16201 tgaatacctt taaaccagta aaagtcgaat tgaactatgt caatcaaacc gtggctcttg
    16261 ttaccagtaa cgtaaaaggg caaaaccaag aaatcactgg tgaaaccact ttgacaaaag
    16321 aagacaaaga taccggtaat gaaactcaag gtaaagctga gtttaaagga gctgaatata
    16381 ctctctttac tgcaaaagat ggtcaagcgg tcaaatggag tgaggctttt aaaccagagt
    16441 tagtgaaggg aacgaaagct tctgatgaaa cggtgacttt gactttagat gaaaagaatc
    16501 aagttgctgt taaacactta gcgattaatg agtattactg gcaagaaacc aaagcacctg
    16561 aaggatatac cttggatgaa acaaagtacc ctgtttccat caaaaaggtg gatgataacg
    16621 aaaaaaatgc cgtgattacc cgagatgtta cggcaaaaga acaagtcatt cgctttggct
    16681 ttgatttctt taaatttgct ggatcagctg ctgggactgc cgaaactggc ttcaatgatc
    16741 tgacctttaa agtatcgcca ttggaaggaa ccagtgagat tacaggtgcc gaagatgaag
    16801 caattacagc ttataacgag caactaggtt ttgatggcta tggcaagttt gaaaatcttc
    16861 cttatgggga ttatttactt gaagaagtag aggctccaga aggatttcaa aaaattacgc
    16921 cattagaaat ccgttctact tttaaagaaa ataaagcgga ttacactaag agtgaataca
    16981 tttttacgat taccgaaaaa ggacaaaaac aaccaattaa aacggtgacc gttccttacg
    17041 agaaactgac gaataaagca ttttctgtta gcttgaatcg tttgatgctc tatgatttac
    17101 ctgaagaaga agatagctta acttctcttg ggacttggaa agacggaggc aaagaattga
    17161 cagaccttga ttctaccgag ctagttgata aattgagcta taacttgcat gaaatcaaag
    17221 aagactggta tgtggtagcg caagccattg atgtggaagc gacaaaagct gcccaagaaa
    17281 aagatgaaaa agccaaacca gtggtaattg ccgaaacaac cgcaacgttg gcgaataaag
    17341 agaaaacagg aacttggaaa attttgcata aattaaccgc agaacaagtt ttggataaaa
    17401 ccatcgtctt attcaactat gtgtatgaaa acaagaaacc ctatgaagca ggtgatgatc
    17461 cagtagcgaa agatgctagc ttgaacaatc aagcccaaac tgtcaattgt acggtggaac
    17521 gccatgtttc catccaaaca aaagcccacc tagaagatgg ttcgcaaacc tttactcatg
    17581 gggatgtggt ggatatgttc gatgatgtgt cgattaccca tgatgtactg gatggctcaa
    17641 aagaagcttt tgaaacaatt ctgtatgcat tacttccgga taatacgacc aaagaaattt
    17701 ggaaatctgg caaaattgat tatgaagtga atgacaaaga attcactaaa accgtacttg
    17761 cggaaaaagt ggacacaagt aaatatccag aaggtacaag ttttaccttc aaagaaatta
    17821 actatggcaa agacggaaac atcaatggga aacacaatga ggatctaaaa gaaaaaactc
    17881 aaactttaac acctaaaaaa gttccgacaa caccaagtgc accagaacaa ccggaaatac
    17941 caactgttcc aagtgactct caagaatcta gtcctacagt aaagacattc ccacaaacag
    18001 gggagaaaaa ttctaatgtt ctgctgttta ttgggtttac cttgatcttt gcgactgctg
    18061 ggtattattt ctggaatcgc cgaaactaag gtgatgtgat gaaacaacta aataggaaaa
    18121 accaacgtgc gcgaccgcct cctaaaaaaa gtggtcgcgc tatttttgta tccaaaacta
    18181 aaaatgaaaa gaggaaaaga agatggaatt gaaatttgtt gtgccagata tggccgaaac
    18241 ctttggaaag ataaggtatg ccggcgaagg cgaagtttta actgaaggat atgggcggaa
    18301 tactacagta attggtcgta gttatcattt gtattccagt aagcaacgag ccgatgatat
    18361 tgaagtggtg gtagctgcgg aagccggcga aaaggatttt gatcaagatc aaccactaaa
    18421 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttaccga
    18481 ctacgtgttg tatgtcgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    18541 agaaggtatt gttgtagatt caggactaac gtttgggaaa ttacgatttt ccgcattacg
    18601 ccgagaagta cggaagcaaa atgaggatgg aacggttagt aacgaggtaa aagaacggac
    18661 ttataatttg aaatcctctg cgcaaggtcg aatgatccaa gttagtatcc cagcaaatgt
    18721 acccttacgc gagtttgcct atgacgcaga ggtagagttg gtcaatccaa tcatggatac
    18781 ggtcgccaat tatgttttcc gagaaggaac tactgtcaat tggttcatta aggccgatga
    18841 tttagtcttg aaacgacaac cagatcaggg aaattctaca aaccaaaatg aaggaaagaa
    18901 ataggtgaag cgtatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    18961 aaaaggtgag ctaacggagg aacaactcac taaagcatta acctatgatg aaaaaatgtt
    19021 tctgattatg catcaagggt tactggaagt aggtaacgat gtcgaagaag attttgatgt
    19081 actgaattgg ttaggggaag aagagtcctt ctttatggtt atcgaagtaa atgaaagtct
    19141 atgtcgagaa gccgaaaccg tattggaaga aattggtgtg gaaatgccgg atgccattga
    19201 agttttttta aagcaactag tggaaacgaa acaactccct atagcagtaa atgactagca
    19261 taactgcttg aaattggatc aatgaaatcc tatgaagctt ttttcgattg gaggtggaaa
    19321 ccataaaaat gtacaaaggg catcgtatac gggcaggaga tcagcacttg gtttatcact
    19381 ttgttcttgg ctggctactc gctctgttca tcggttggat gagcgtcttt tattttcaag
    19441 agcttagaca atttgatatt tctaagctat cactttctac tatagaaatc gttcgttcta
    19501 taaaagattt aatctatttg ctaggaagtc tcgttctttc aggaagcacg atgttgcttt
    19561 atatacactt ttttcaggat cattggcgca gtttgtggca tcgacaaaag ctagcccgga
    19621 tgattctaga aaatcattgg tacgaagtaa aacaaactca gagtgaagga tttttcaaag
    19681 acctcaatag tagtcgaacc aaagagacaa tcagttactt ccccaaaatc tattaccgta
    19741 tgaaggatgg tttactttct attcgcgttc aaatttcact gggaaaatat caagatcagc
    19801 ttttaaagct ggaaaagaag ttagaaagtg gtctatattg tgaactagta gaaaaggaac
    19861 tcaaagactc ctatgtggag tacactttac tgtatgatat aattgccaat cgaattggga
    19921 tagacgaagt agtggcagaa agtggggctt tgcgattgat gaaaaatcaa gtatgggctt
    19981 atgattcttt acctcatatg ttaatcgctg gcggcacagg tggtgggaag acttactttt
    20041 tactcactat catcgaggct ttattaaaat ctgatgccga gctttttgtt cttgatccca
    20101 aaaatgcgga cttagccgat ttaggtacgg taatgcctca tgtctattcc caaaaggagg
    20161 aaatttctgc ttgtgtggaa gatttctatg agcgcatgat gactcgtagc aaggcaatga
    20221 aagaaatgcc caactacaaa acaggagaga attatgcgtt tcttggactt ccaccaaact
    20281 ttttaatctt tgatgaatac gtggtttata tggaaatgtt aacgacgaag gaaagtgcgg
    20341 tgattttgaa taagctcaaa caaatcgtga tgttaggtcg tcaatctggt ttctttctga
    20401 ttctagcttg tcaaagaccg gatgcgaaat atttaggaga cgggattcgc gatcaattta
    20461 atttccgggt ggctttgggt cgtatgagtg aactgggata ttccatgatg tttggcgagg
    20521 ttgataaaaa attctttatg aagcgtatca aaggtcgagg ctatgtagat acgggaggga
    20581 gtgtgattag tgagttttat acgccacttg taccaaaagg atatgatttc ttaagggaaa
    20641 ttagcgacat tggtggatta aacgttaata cggaaggata aaataatagt atgtagccta
    20701 ggagtgtcaa gaatcctggg ctttttttac gaggtgattt atgacatttt tagatttatt
    20761 cgctgggatt ggtggctttt gcttaggtat ggaacaagcg ggtcaccagt gtatcggctt
    20821 ttgtgaaatt gatgattttg ctcgccagag ttataaggca atccatgata caagtaagga
    20881 ggtggaaatg catgacatca caagtgtatc agacgagttt attcaatctc tcggaccagt
    20941 ggatatcctt tgcggtggat ttccgtgcca agctttttca attgcgggaa agcggcaagg
    21001 attttccgat actcgaggta ctttattctt tgaaatcgct cggttcgccg ctattctcca
    21061 acctaagttt ttattccttg agaacgtccg gggattactc aatcacgaag gaggggctac
    21121 gttcgagacg atcctccgaa cgctggatgg attggggtat gatgtggaat ggcaagtgct
    21181 taactcaaag gcctacgttc cccaaaaccg tgagcggatc ttccttatcg gacattctcg
    21241 agacgcatgt accgaacaag tatttcctat cattggatcg tctccaacat ctgatcaaaa
    21301 catcagaaac ttattaaata tcaacccttc caatcgagga atggggggac aagtctatgg
    21361 gtcagatgac gtagcgccta ccttgaccgg tgatgaagga atcaaaatcg cattgccggt
    21421 agatgatgga atttctgtag cgggtatgtt actgggaaat tttgaacaag gcaatcgggt
    21481 ttatgagata actggaacag cgcctacttt gtcaacgaaa caagggggaa cgaagatcat
    21541 gcttcggaaa aacgaaacag aatatcaaga ggtgaagcca gggaacagtg tgaacctggc
    21601 ttttcctaat tccacaagtc gtcgaggccg tctgggaaaa caaagtgtgc ataccttatt
    21661 aacaggagac caacaagcag tcgtcacgga tcagtatcaa attcgaaagt taacacctag
    21721 ggagtgctgg cggcttcagg gctttccgga ctgggctttt gatcgagctt ctcaagtcaa
    21781 ttcagatagt cagttgtata agcaggctgg aaattctgta acagttccgg tcatttttga
    21841 tattgctaga agattaaagg aggtaaaaac agatgattta tgatgagttg attggggaat
    21901 ctactgggtg attggcaaga ttcagtcaga tccggaattg gaagaagaac tgtaccggct
    21961 caattttgat attcgcaaaa acggggtgaa agtccctgat gatccgtatg taattgatga
    22021 ggaaacggat gctcgaatgg agctgaatca agtcatcagt gaattggaac gaattgcgga
    22081 ctttgctaaa gaacctgata tccgccaata cttgtttgag ataaaagcag agttggaaat
    22141 tgagggtatt accgcggagt aacggccaaa taaaccactg ataggcactg accgcggggg
    22201 atttgcgaag cagaaatcct ccgtggtgct gtgcctgttt atggacggcg ttagccggcc
    22261 atgatgtctc accccccgta tctaacaggg gggtacaaaa acacaaaaaa taagcaagca
    22321 acgataagga atggctgtcc tatcaaggat aataagcaat ttgtgtagat gttaactgat
    22381 ttgaaaaagt taacacctta aatttggagg tgagaacttg gcacaaagga atttagatta
    22441 tcggctatta aaagaccggc gtaatgaata tggcgtttca caaaataaat tagctactgc
    22501 ttgcggactt agccgcccat acctaaatca gatagaaaac ggtggcgtaa tcgcctctac
    22561 aaagaccatg agaaaaattt ttgatcaact ggaaagtttc aatcccga
//
LOCUS       DAZKSR010000049        22464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000049 DAZKSR010000000
VERSION     DAZKSR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.049"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACG8XG_001622"
     CDS             144..542
                     /locus_tag="ACG8XG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0773543.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="ACG8XG_001623"
                     /pseudo
     CDS             526..1220
                     /locus_tag="ACG8XG_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="ACG8XG_001624"
     CDS             complement(1814..2701)
                     /locus_tag="ACG8XG_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0773544.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="ACG8XG_001625"
     CDS             2886..3725
                     /locus_tag="ACG8XG_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0773545.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="ACG8XG_001626"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="ACG8XG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0773546.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="ACG8XG_001627"
     CDS             5193..5549
                     /locus_tag="ACG8XG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773547.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5594..6709
                     /locus_tag="ACG8XG_001628"
     CDS             5594..6709
                     /locus_tag="ACG8XG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773548.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="ACG8XG_001629"
     CDS             complement(6735..7175)
                     /locus_tag="ACG8XG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0773549.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="ACG8XG_001630"
     CDS             7335..7841
                     /locus_tag="ACG8XG_001630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0773550.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="ACG8XG_001631"
     CDS             8009..9922
                     /locus_tag="ACG8XG_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0773551.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="ACG8XG_001632"
     CDS             9927..10928
                     /locus_tag="ACG8XG_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0773552.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="ACG8XG_001633"
     CDS             complement(11130..11336)
                     /locus_tag="ACG8XG_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0773553.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="ACG8XG_001634"
     CDS             complement(11982..12830)
                     /locus_tag="ACG8XG_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0773554.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12994..13896
                     /gene="gnd"
                     /locus_tag="ACG8XG_001635"
     CDS             12994..13896
                     /gene="gnd"
                     /locus_tag="ACG8XG_001635"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0773555.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="ACG8XG_001636"
     CDS             13898..14899
                     /locus_tag="ACG8XG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0773556.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="ACG8XG_001637"
     CDS             14896..15180
                     /locus_tag="ACG8XG_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0773557.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="ACG8XG_001638"
     CDS             15213..16010
                     /locus_tag="ACG8XG_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0773558.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="ACG8XG_001639"
     CDS             16024..16857
                     /locus_tag="ACG8XG_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0773559.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="ACG8XG_001640"
     CDS             16875..17732
                     /locus_tag="ACG8XG_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0773560.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="ACG8XG_001641"
     CDS             17754..18263
                     /locus_tag="ACG8XG_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0773561.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="ACG8XG_001642"
     CDS             18300..18701
                     /locus_tag="ACG8XG_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773562.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="ACG8XG_001643"
     CDS             18813..19166
                     /locus_tag="ACG8XG_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773563.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="ACG8XG_001644"
     CDS             19200..19643
                     /locus_tag="ACG8XG_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0773564.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="ACG8XG_001645"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="ACG8XG_001645"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0773565.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="ACG8XG_001646"
     CDS             20738..21451
                     /locus_tag="ACG8XG_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0773566.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="ACG8XG_001647"
     CDS             complement(21658..21807)
                     /locus_tag="ACG8XG_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773567.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="ACG8XG_001648"
     CDS             22203..22403
                     /locus_tag="ACG8XG_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0773568.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3451 c         4641 g         6626 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aatt
//
LOCUS       DAZKSR010000050        22455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000050 DAZKSR010000000
VERSION     DAZKSR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.050"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..243)
                     /locus_tag="ACG8XG_001649"
     CDS             complement(<1..243)
                     /locus_tag="ACG8XG_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0773569.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLND"
     gene            complement(334..786)
                     /locus_tag="ACG8XG_001650"
     CDS             complement(334..786)
                     /locus_tag="ACG8XG_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0773570.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(799..1209)
                     /locus_tag="ACG8XG_001651"
     CDS             complement(799..1209)
                     /locus_tag="ACG8XG_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773571.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1484..1696
                     /locus_tag="ACG8XG_001652"
     CDS             1484..1696
                     /locus_tag="ACG8XG_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0773572.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1699..1911
                     /locus_tag="ACG8XG_001653"
     CDS             1699..1911
                     /locus_tag="ACG8XG_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773573.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2051..2206
                     /locus_tag="ACG8XG_001654"
     CDS             2051..2206
                     /locus_tag="ACG8XG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773574.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2203..2427)
                     /locus_tag="ACG8XG_001655"
     CDS             complement(2203..2427)
                     /locus_tag="ACG8XG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0773575.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2505..2693
                     /locus_tag="ACG8XG_001656"
     CDS             2505..2693
                     /locus_tag="ACG8XG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773576.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2832..2960
                     /locus_tag="ACG8XG_001657"
     CDS             2832..2960
                     /locus_tag="ACG8XG_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773577.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3019..3354
                     /locus_tag="ACG8XG_001658"
     CDS             3019..3354
                     /locus_tag="ACG8XG_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773578.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3587..4528
                     /locus_tag="ACG8XG_001659"
     CDS             3587..4528
                     /locus_tag="ACG8XG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0773579.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4530..5420
                     /locus_tag="ACG8XG_001660"
     CDS             4530..5420
                     /locus_tag="ACG8XG_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0773580.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5463..6263
                     /locus_tag="ACG8XG_001661"
     CDS             5463..6263
                     /locus_tag="ACG8XG_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0773581.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6278..7129
                     /locus_tag="ACG8XG_001662"
     CDS             6278..7129
                     /locus_tag="ACG8XG_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0773582.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7195..7329
                     /locus_tag="ACG8XG_001663"
     CDS             7195..7329
                     /locus_tag="ACG8XG_001663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773583.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7330..7791
                     /locus_tag="ACG8XG_001664"
     CDS             7330..7791
                     /locus_tag="ACG8XG_001664"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0773584.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7817..8269
                     /locus_tag="ACG8XG_001665"
     CDS             7817..8269
                     /locus_tag="ACG8XG_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773585.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8375..8569
                     /locus_tag="ACG8XG_001666"
     CDS             8375..8569
                     /locus_tag="ACG8XG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773586.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8600..8893
                     /locus_tag="ACG8XG_001667"
     CDS             8600..8893
                     /locus_tag="ACG8XG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773587.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8824..9363
                     /locus_tag="ACG8XG_001668"
     CDS             8824..9363
                     /locus_tag="ACG8XG_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0773588.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9438..9905
                     /locus_tag="ACG8XG_001669"
     CDS             9438..9905
                     /locus_tag="ACG8XG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0773589.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10652..10882
                     /locus_tag="ACG8XG_001670"
     CDS             10652..10882
                     /locus_tag="ACG8XG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773590.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11041..11874
                     /gene="terS"
                     /locus_tag="ACG8XG_001671"
     CDS             11041..11874
                     /gene="terS"
                     /locus_tag="ACG8XG_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0773591.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11867..13282
                     /locus_tag="ACG8XG_001672"
     CDS             11867..13282
                     /locus_tag="ACG8XG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0773592.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13294..14823
                     /locus_tag="ACG8XG_001673"
     CDS             13294..14823
                     /locus_tag="ACG8XG_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0773593.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14741..15793
                     /locus_tag="ACG8XG_001674"
     CDS             14741..15793
                     /locus_tag="ACG8XG_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0773594.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15795..16112
                     /locus_tag="ACG8XG_001675"
     CDS             15795..16112
                     /locus_tag="ACG8XG_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773595.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16239..16862
                     /locus_tag="ACG8XG_001676"
     CDS             16239..16862
                     /locus_tag="ACG8XG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0773596.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16877..17767
                     /locus_tag="ACG8XG_001677"
     CDS             16877..17767
                     /locus_tag="ACG8XG_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0773597.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17790..18005
                     /locus_tag="ACG8XG_001678"
     CDS             17790..18005
                     /locus_tag="ACG8XG_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773598.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18017..18358
                     /locus_tag="ACG8XG_001679"
     CDS             18017..18358
                     /locus_tag="ACG8XG_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773599.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18358..18729
                     /locus_tag="ACG8XG_001680"
     CDS             18358..18729
                     /locus_tag="ACG8XG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773600.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18722..19117
                     /locus_tag="ACG8XG_001681"
     CDS             18722..19117
                     /locus_tag="ACG8XG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0773601.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19119..19496
                     /locus_tag="ACG8XG_001682"
     CDS             19119..19496
                     /locus_tag="ACG8XG_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0773602.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19497..20105
                     /locus_tag="ACG8XG_001683"
     CDS             19497..20105
                     /locus_tag="ACG8XG_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0773603.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20105..20446
                     /locus_tag="ACG8XG_001684"
     CDS             20105..20446
                     /locus_tag="ACG8XG_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773604.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20688..>22455
                     /locus_tag="ACG8XG_001685"
     CDS             20688..>22455
                     /locus_tag="ACG8XG_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0773605.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMI"
BASE COUNT         8048 a         3304 c         4595 g         6508 t
ORIGIN      
        1 atcatttaaa tcttctttct ctttttttaa taaaataata tcatttttta acatagtaat
       61 ttgtttattt aaatctgata gtgtttcttc cttacttaat ataaatcttt ctctatcatg
      121 taatttattt agtttttcat catttttttg ttctaattca taagcttctt tttttattgt
      181 gttagctttc gacagtttag tgattcctat aattaataaa ataagtccaa tcaataataa
      241 cattcaaaac tcctcatttc tttgatataa ttattgtaga gtttactcag aaatgaggaa
      301 agagtccatg tgaaagcatg ggctttttta tttttaatgt tgtaatacta aatgttcaag
      361 attatgagga agccctaaat attcaataat ttggaactga ctcatatcag ataaatcttg
      421 ttcatctatt gttctagtta acatttcaat tgcaaacata ttagcttcag cttctatacc
      481 ataaatcata caactaaggt tgtttgctct aaaagctgct gtgcaaatac ctttatggag
      541 tctaacgtgg gctatttcat gccataatac aaatcttttc atagaaggtg ataggttttg
      601 attaattaat atcacataac atcttttatt gtgtatcgtt tgtcctaatg ttttattccc
      661 taaatcagtt tcaattaaat ctatatcaga atatttgatt attttatatg gattaatcgc
      721 atcagatcca aactctacaa ataatttttc tatttcttct aatatccagt atttgtttaa
      781 atgcatacca aagcccacct attgacgata ttttttagga gtgaattttt tctttgcttt
      841 aactttggtt aaggcaagtg caagttgaat ggcatcgtat aatttttcct tatcttcatc
      901 gtctaaaggt tcaccataat aatttgtttc agcatctgaa ttcaagcctt caagtaattt
      961 ttcagcttgt aaagcaatat cttgtttatc tttttctgtt aaatcataat aatggcgttt
     1021 atcagtacgt cctaaaagat aatcagttga tacattaaag taatctgcaa ctttttgtat
     1081 tttatctcca gatggagtac ttttattcca tctagacatg cttccatttc cgaaatttaa
     1141 ctttctttca agttctgcca acgtcattga acgttctttt aataagattt ttatacggtc
     1201 taatagcata ggatatgcat tcctttcaaa ataccgacaa aaaaagtatt tttctgcaaa
     1261 ttatctattt acaaatagag aatttgcagt tatactagtt ttgtaagcaa gatatttacg
     1321 gcatacaaag actaaaagca ataaactaat agtctcccca gacatcagta agctcgcttt
     1381 ttgtgcgctg tattttgtat gcttacataa tagcatattt gcagtttttg tcaaacttat
     1441 tagacaaaaa tattagaaaa tattcgataa gggggtatga agcatgactt taaaacagca
     1501 agtattaatt gctttagtaa agaaaggttg gagtcaaaga gagttagctc gacgaatggg
     1561 tatatcaatt acttatttac gagatatctt catcgaaaaa agaaaaccta aggaacgttt
     1621 aaaacaaatc gaagaacttt tggatatcaa gttagaagta gattctaatg atcagccatc
     1681 agaacaggag gcgtaaaaat ggtacaacgt ttgagcaaag cggagtttaa agagaaatat
     1741 aagtattctg aaagtactta tcaaagaaga atgaaggaat ttaaaaaatc acgttttagt
     1801 gaggggtata acgcagttac ttccaaagaa ataacaattg atgtcgattt atatgaacag
     1861 tttagaacat ggcgttctaa aaatcgattt agagttagag ctgtttgtta atagctaaaa
     1921 aatctaaatt caccacttct gaaaagagga gttgctgctc tcctttcaag tttgccaaag
     1981 tccacgtttt ttcattttgg caactcctct tcttggaggt ggtgacggcg agagacgaag
     2041 gagggatgac atgaaggaag aatcaaaaat agggattttg aatgagaaca attacacaag
     2101 attaattatt gaaacagaag aagggaaaaa agtagcagaa gttacattga ccgatgcaac
     2161 tcctgctgac ggttatcgta ttcgattaac tcctaaatat gattaacctt ttggtgggtg
     2221 tggatcgttt ccgtgactat cacggctttg aatttttcca tctctgccat gaataaatag
     2281 ctcagatcct tgatttttag aaatctctct agcgattttt actgcttctg attttgtatt
     2341 agtatgtgca gtcgctttag aatttccagc acctttgaca ttccaaccac cattggaatt
     2401 aggtacaaca tgttgatttt ttcccatagt tttcacctcc atttctatag aaatatttta
     2461 tcaaatagaa atggagaaat tcaataaacg aaaggagaag tgatatggca tataccttgc
     2521 aacaagaaca tcaaattctc ggtttgatta aacaacgcag gaaacaatta caagatgacc
     2581 gtgcagcgct tagaaaagcc gatgagctat cagataggca agctgaacta attgcttctg
     2641 aacttgagga tttgagaatg ctagaaataa aaaataggga gattagatta tgaagaagac
     2701 agacacactt tttataggat tcattttggg gttattagtg attgtagcgc accaaagtat
     2761 tatcggggga agcttgttcg cagcattgat ggttttaatc aatctgcttg attcaaaaga
     2821 aaggagcaac tatggcacga gaagaagcgc taaaaatcgg taaagtgatt gctgataatc
     2881 ggtggggaaa tagccgccct attattttaa gcaagcaaca tatcgaaaag caaaaagcat
     2941 ggcaacaaat aaaaaagtga ctctgccagc cagcaaagag tcacatacaa atttataaaa
     3001 ctaggagaat tatatcacat gaataagaaa attgaaaatc ttatcgaaga attgaaacgt
     3061 gaatgccaaa agcaaggtgt ttctattatt tgtacagccc aaaaagaagg ggaacttaaa
     3121 agtctcgttt atggtgagac aacagaaatt ttactttgtc ttgctatgca agaagaacat
     3181 ttagatgaaa atctcccatt gtcagcacat attatgcgta gaattgctgt ggatgcttac
     3241 gaacaagcga aaaatgaaga agaaaatcaa ccttcaaatc atacttttgt tattaataac
     3301 aaggaagatt tagctgatgt gatgactcgt attctcaagg gggaatttca atgatgaatg
     3361 aaaaaacaat tgatctactc attattttat ttttcacgtt agtgattgta gcgaaaagtt
     3421 atgtaatctt tggcttgtta ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca
     3481 gaaaagaggt taaacatgaa cgagttagca aatcttgaca actatttaac tgatcctgat
     3541 tatgctaaac caccatatga agcaccaatt gatgaggagg atgaagatga gtaaatctac
     3601 cttagaaatg agccatcaag aatggcttga agaccgtaag aaaggcatcg gaggttcgga
     3661 tgttggaaca attttaggat tgaataaatg gaaatctcct tatcaactat ggctagaaaa
     3721 aacaggacaa gtagtacttg aagaatcagg aagtgagccc gcttattggg gtaatgtttt
     3781 agaagaagtg gttgctaaag agtttcagga acgtacaggc aaaaaagtac gcagaagaaa
     3841 ccaagtattt gaacatccat tacatccatt tttaagagca aatattgatc gggaagtagt
     3901 gggagaaaat gccattctgg aatgcaaaac agccaatcaa tttctcggta aagaatggga
     3961 aggcgaagag gtaccgctta gctatctctg tcaagttcaa cattacatga acgttctaaa
     4021 caaagactat tgttacatcg ctgtcttaat cggtggtcaa aaatttatct ggaagcggat
     4081 tgaacgagat caagagctga tcgatacaat cactgaacaa ttagtagagt tttgggaaac
     4141 aaacgttctt ggaggtatcg agccaattat tgatggtagt caagcgacag ctgatttctt
     4201 gaaagagaag tatgcagatg ttgaagacgt tcaaacgacg ttaccaattc atttcgatga
     4261 actagttgaa cagaaaaacg aactcaaacg gactaagaaa gaaattgaat cagctatccg
     4321 tcaagtagac aacgagatta tcagcgagct aggtaaacgt gaagccagta tcggtatcac
     4381 tcaaaaaaac atcatcagtt ggaaatttgt ccgtacgaga cgtatgaact cgaagaaact
     4441 agcagagaaa tatccagatg tcgcaaatga tgaagagatt tataacgtta ctgaatcaag
     4501 aaggctaacc gaaagggaga tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat
     4561 tggcagcaaa gccacagaaa caagttgcac caggtcaatt aggacttaaa gctttgatga
     4621 atacaccaac aatgagaaag aagttcgaag aggtgcttca tgacaatgct aatgctttta
     4681 tgtcgaatgt tatgactctt gtatctaatg acagttatct tgcagatagt gaaccgatgt
     4741 ctatcatgag tggtgcgtta actgctgcaa cattaaatct tgggctagat aagaatttag
     4801 gttatgcata tttagttcca ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga
     4861 aagctcaatt catgcttggc tataaaggat atatccaatt agcccaacga tcaggtaaat
     4921 acaaagcatt aaatgtgatc gaagtttacg aaggagaact aaaaagctgg aaccgactga
     4981 cagaagagtt tgagtttgat ccaaatggta gaacgtctga tgaagtcatt ggatatgttg
     5041 gctatttcga gttactgaat ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg
     5101 aagctcatcg gattgctaac aataaagatc gcgataagac aaagttaagt ggtgtgtggg
     5161 catctgatta caatgcaatg gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg
     5221 gaatcttgtc catcgaaatg caagaagcca ccacatcgga tgagagagtc caaagggttc
     5281 aagaagacgg cagcattatt gctgaaacag aagttgaaga agatattcct gaaagaaaag
     5341 aagcagaggt tatatctgaa gaaaatgaag atgtacaaac tggattattt gatgcatcta
     5401 atccgccgtt aaacaaataa tgagggagct ttctcccttg cttttctaga ggagaaatac
     5461 gaatgaacac aggatatgtc aaattacatc gaaaagtgat gaattcattc gtttggacca
     5521 atccttatat gtataaatta tggaacttgt gtttgatgaa agcgagtcat gagaatcgca
     5581 agatactttt taatggaaaa gagatacaag tgaacagcgg agaattcgtc acagggcgcg
     5641 atgctatcac atctgagatg aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt
     5701 ctgtatggag atggctaaaa caatttgaaa aaaacggaat gttgaacatc aaatcaacta
     5761 cgaaatacag cgttatatca ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa
     5821 tgaacatcaa acgcacaaca agtgaacaac aagtgcacac aaacaagaat gaaaagaatt
     5881 ataagaatga aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg
     5941 acaccgactc agtttactac attctcgcgg aggagttatt caaacagatt tgtcagaatc
     6001 aggaaatcaa aaagccgaat ctgcaaagtt gggcagatga cattcggaaa atgatcgaga
     6061 tcgacaagcg gaccgaaaac caggtacggg gaatgattga atggagtcag cacaatgtgt
     6121 tttgggcatc gaatatttta tctgctaaaa aattgcgaga aaaatacgac acaatggcag
     6181 cgcaagcaaa tcgtgattat aaaacaaaac aaactaaaac gcttgaatac gagaaattta
     6241 gcacagatga gttgcctatt tgagaggagg cgtaggcatg gaaactgttg gggaaatcat
     6301 ggaaaagctg atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc
     6361 tttgtatgga tggcgtacga agaaccctga tggctcagaa cgttgtaagc caacctgtat
     6421 gcaatgtggg tataaagcat tgcgagttca ggaagactta caaacagaac gaatttacaa
     6481 cgagagcctg aaagcaagag caatcaattt tttcaaaggt ggttctgttg tgcctaatca
     6541 agcgttgttt gattgcacat tgcagaatta tcaaattgtc gatcaagaaa caagacaagc
     6601 ggttgaagta accaaacgct ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt
     6661 tttaactgga aaacaaggaa caggtaaaag tcacctagcg atggcagcgg cttgggaagt
     6721 cttgaagcga tcaaactacg ataaaaaaat cttatttatc gggttacagg aaatgctgga
     6781 tcaaatcaaa ttttcgtaca acaatcctga actcagaaaa acgattgagg gatcgttgat
     6841 tgcagatatc aaaacagcgg atttagtcat cattgatgat atcggttcag aactaggaaa
     6901 agatgcatca gatagtcgag cgtttggcat aaacacgcta aattcgttct tggatgcacg
     6961 acagaaccta gcaacgatta tcacaacgaa cttgcttggt gaagaactga aaaaagctta
     7021 cggtacgaga acaatatcaa gaatgtttgt caactctgat ggatttacga tggtattttc
     7081 tcaaacagca gacaagcgca taaaaccagt caaaggagcg atcgcatgaa tttgactaaa
     7141 caagaaaaaa gaatctgtcg tcagataatg ttgcaaatta taggtgatcg tgacatggac
     7201 gaattatctc aacaagagag tcgaatggtt cggaagataa gtaagctaat tatttatcgg
     7261 gatacagaaa gatttctcac tgttttaggt aacgtctacg agaatccaga gttattggag
     7321 gtagagtaat tgccaaaaat attagatgca tgttgcggta gtagattgtt ctggtttgat
     7381 aagaacaatt ctgacgtaac atttatggat tgtcgtcagc agtatgaaga attatcaaca
     7441 ggtcatgtga tcaatgttga tccagatgtg gtcgcagact ttcgcgatat gccttttgaa
     7501 gatgatgtgt tcgatatggt tgtatttgac ccgccacact taagacatgt cggcgaaaac
     7561 tcatggcttg ccaaaaaata tggaaaatta gacgaacttt ggccagaaga catcaggcaa
     7621 ggttttgcag agtgtatgcg agttttacga ccttcaggaa cattaatttt taagtggaac
     7681 gaggaacaga taccactttc tgatgtgtta gaggctattg gagaacagcc attgtttggt
     7741 aataaacgaa gcaaaacaca ttggcttgtg tttatgaaaa aattcagcta acggaggaaa
     7801 caaaggagga aaagtaatga agattgtatt aaataaatgt tttggtggct ttgggttgag
     7861 ccatgaagca aaaatggaaa tttttaaaag aaaaaatatt gaagtattcc catacatcaa
     7921 taactttagc tatgactcag atgatgaata cacgagatac acaggccaaa agctaggttc
     7981 gatggatttt atctactatt tcaaaaaaga cccgaaaatc gacaaagtta caggaattta
     8041 tagcgaaatt gaccgactgt acggaattgc tgatgattcc agtttttcaa gtgattcaaa
     8101 cagaggtgat aaggacctcg ttgctgttgt cgaaaaacta ggaagtgagg ccagtggacc
     8161 atatgcaagt cttaaagtcg ttgatatacc cgatggggct gagtgggaaa taagtgatta
     8221 tgacggcgtt gaaactgcac attatggttt tcaaacaggt agtatttagt ccactaatga
     8281 caactaaaga ggaggcagaa ggatgagtga agatgaatac tttgaacagt agtctattaa
     8341 cgacagagat ttttaactgg aaaggggtgt agtcatggac gcatcggaag tagtaaataa
     8401 aattaatagt ctcaaagagg ttttcggaga cgtcgaagta gtgataaacg cacaaggcta
     8461 tgacaacgaa ttttttaagc agattaccga cattagcatt cagcaaggaa tggaagacga
     8521 agacggaaac tttatcgatg aagtagccat tttagtgact tgcgagtaag taattgacag
     8581 ctaaagagga ggaagcggaa tgaaactaaa agacggattt tactccagcg gccacggcat
     8641 cggcggttta atgctagata tgccgacaaa gaatcctaaa acacgtaaga aaccaaaatt
     8701 caaagtcggt gacatggttc gctgcgaagc agaaggattc atctatccat ttcgtggata
     8761 tgtagaacac gtctataatc actcagcaat cattcgcatt gaaaacacga tggaatgtga
     8821 taagtggtta gcgaaaagca aagagaattt agcagtggtg agattggtgg acatggaagt
     8881 tataaaatct taattttgct tttttacaac tatcctacaa caatgagcgt tattccacaa
     8941 ctggaaggag aagaggcaat tgtgaacatt ttagagaata tagatattaa gcaaacaagg
     9001 aaaaatgcta gacgattact aaaaagatac agaaatttag aacgtttggt cggaccagtg
     9061 aaaatagact tttccgtgat gactgttacc aaaaatttga aattcacaat tgacagtcaa
     9121 aacgaagaaa tcattgaagc gataagtact cgagattcgg ttatcgaagc attaacacgg
     9181 ctaagtagaa tccatttcca ggtactttat tatagttatt gttttcctaa taagatgtca
     9241 atgtatcaaa taggagaaaa attaggatat tccgatagaa ctatcgagag aatgaaggca
     9301 gtagctttgg ttgaatttgc tgaggcgtat aaatcaggag aactcatttc acgtacaaaa
     9361 taaaaaaagt cggattcctc cgactatgaa taatatttcc gacataagta ttatatcata
     9421 attgggggaa tcagaggatg gtacttttcg atgtaaagaa atatgagaca ccaagcgcga
     9481 aagatgttga tatggaacgc acaaaacata atgtcgctgt gtttctttca gcatatctat
     9541 cagctagatg tagagtaggg caacatcgag aaccaaaagt gacagcatct tattccttgg
     9601 ttccaccttc tacagctgat catatatttg aagcagaaag aatgctgatc gataaagaag
     9661 aagcacagga agaatttgag tatctgcata aattgtttgt tcgaggctat tcagcgatac
     9721 agcatccgca taagcctgat gtgactgaaa ggcgcaagaa gatattctat gatcgttata
     9781 tcaatggtct gcccatttat gtaactgctc aaaggaataa tactagcgaa gaatcggtta
     9841 aagtagaatc aaacagaatt atcatccaat ttgcttcatc gttagaactg gttgctttca
     9901 agtagccagt ttttacactt tttataccct tttattacca atttggtttc cattttatac
     9961 cttttttata ccaatcactt acctattcaa cgttgtatta tgatagtgtc gaaaaattag
    10021 gaaacaggat cgacaaaata aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc
    10081 ttcgataggc agcaacggaa atattaagaa taaggatgtg aatttcaact ccttctaaat
    10141 tgttcttatt atctatcatc cgttgctgtc tattgttttt ttaattattc acacgataac
    10201 taaaggtaag tgaagagaaa tgattccatt aataatttca atttttgcgc tttgtcttaa
    10261 tgtatatatg attggattta aaaatgggca aaataaaaaa tagtagcagc caagaataat
    10321 tttatagtgt cactgtggcg gaaagggtag acgctaagca tgtgtgctag gtcaatgctt
    10381 cggcaaccat gcaaggttcg attccttgcc agtgactttg gcaaccgagg catcggcggt
    10441 ttaaaaatat aggggtgcgc aatttcgtac gcgttttgtg catcgtgtag gttgctatta
    10501 cataactggt taggttagat tgagatttgg gattcggtac aaatgaatcg tcaaatgtct
    10561 caagcacagg atcggaaacg tccctgcctg tgcattacat attagatcac tctttgagtg
    10621 gtctttttct ttacataaag gaggctacat aatgagaaac tactggtatg tatcgctaac
    10681 taatgaatat cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat
    10741 caaagggaag tactctattg tcgaaatggg gagagaagca gaaccatgtg agattgatgc
    10801 gtgcaagctc gtttattgcg gtcatggttt ctatgatgaa ccaaacattc aaaataatat
    10861 taacaagaat ttgagggatt agaatgcaag aaatggctta aaaatctcca atcaggacaa
    10921 atcaagttag acaaagtttc cgatttaaag atattgattg aagctgattt aatgctaaaa
    10981 gatattgaaa attagaaaac aaaactcaac ctaaacagaa tgtgaggtgg tgtgtattga
    11041 atggcaagac aacgtgatcc aagacgtgat gaagccaaaa gaatttggtt agaatccaac
    11101 ggagaaaagc agttaaagga aattgcatct gaattaaatg tttcagattc tcaggttaga
    11161 aaatggaaat cgcaagacaa atggagcgct gaattgatta gtaacgttac caatggcaaa
    11221 agtaacgtta ctaatcaagg tggcgctcct attggtaatc aaaatgctaa aggtaacaaa
    11281 ggaaatagcc gagcttctcc gccagtgggt aataaaaacg ctttgaaaac aggtgagtat
    11341 gaaacaatat tttttgacac gttaaccgat aatgagaagg acatctattc tagtttgaat
    11401 gatgatcctt cttttgcttt gtccgaagaa atacgacttc ttaaaataag acaacttcga
    11461 atgatgaaaa gaatccaaca agctgaagct ggactaaatg atgaagaagt cgaacgattg
    11521 cagcagctaa gaaagattaa aactccgatt gaaaaaaatg gtaaaaagct agaaatcaag
    11581 cgtgaggtta tgcaagatgt gcagattagc agaaaaaaac atcgcaaaat tgatgatatt
    11641 ctttcaattg aagattcatt gactcggatt agcaaccagt tagctaaagc catcaagcaa
    11701 atgaatgaac tttatatgaa tgaatacaga actgatttaa ttaaagctca gactgataag
    11761 atccaagctg agacaaatga aattggcgga aataattcag gtgaagaaat agaagaatgg
    11821 aaacaggcag ttttaaatgc cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt
    11881 tattcctttt gctgatattg gttctgccat tgattattac tatgataaac cagtagcttt
    11941 ttgccaagat attttgcatt tgaatcctga tgaatggcaa gaaaatgttt taaatgattt
    12001 agctgaattt tcaaaggttt ctgttcgttc tggtcaagga gttggaaaaa cagcattaga
    12061 agcaggagca atactttggt tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc
    12121 tccgacaatg aagcaacttt acgatgtact ttgggcagag gtagctaaat ggttaaatga
    12181 tagcttgatc aaaaacttac tgaagtggac aaagaccaaa atttatatgg ttggtgattc
    12241 agagcgttgg tttgctacgg ctagaacagc gactaaacca gaaagtatgc aaggttttca
    12301 cgaggaccat atgttgattg tggtagatga agcttctggt gtgtctgatc caattatgga
    12361 agctattctt ggtacgctat caggttttga taataagctg ttgatgtgtg gaaaccccaa
    12421 taatattgaa ggtgtctttt atgattccca taattcagac cgtgataaat acagagttca
    12481 taaagtatca agctatgata gtaaacgtac aaacaaagac aatatagaaa tgattcttaa
    12541 aaaatatgga aaagaaagtg atgttgctcg tgtccgtatt tttggagaat ttccgaaagg
    12601 tgcgttggat tcatttatca gtcttgaaac agttgaattg gctacagaaa aacaaattag
    12661 tgattcttta gtcaataaaa caaaggttgc tcatattggt gtcgacgtag ctcgatatgg
    12721 tgatgattct acgattctct ttcctagaat tgctaccagg gcattggagt atgagaagta
    12781 ttcaaaacgt agcaccatgg aaacaacagg atatgtcatc aacatggcca agaatctaat
    12841 gagtcaatat ccgagtattg ataaagtgat gattaaagtc gatgacactg gtgtcggagg
    12901 tggtgtaacc gaccgcctag aagaacttat agaagacaaa cattatcctt ttgaggtgtt
    12961 tggagtgaat aacggttcaa catcagaaga cgatttttac gataatttag gtactcaact
    13021 atggggaaac atcaaggaaa tgttagaaga aaatatgaca gcaaatctta acggagaaca
    13081 gcctgttatt gaattgcctt ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt
    13141 caaaatgaca agtagaagtc gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa
    13201 tattggtagt cccgatattg ctgacgcact ggctttagcg ttttatgagc caccaagtca
    13261 ctatcaattt attcaatttt aggaggtgag cttttgatta gtacagtatt atctttagag
    13321 agatataaga agctacgtgt taaatatgca acacaaattg aagatggaat gtttgatccg
    13381 aatggtttta tagaagatat gaaaccattt tttgctgatc gcgaaagaaa atatctagct
    13441 tatactagcg aaaagaatga aatagataat agaccaaagc ctaacactga tattgtaaaa
    13501 gttaataata aacttcatgc tggtatgtat tcaattgtcg tagaccaagc agtcaatcat
    13561 tttactggta tacctatcaa atgggattac gatgtatcgg aacaaaaaag aacactcatt
    13621 caaagattaa aagataaatt cttaaaaaat gatatagaac ttccgacagt tccagaagct
    13681 tttaacaaat taacaagcaa tcttgattcc atgagattcg caatgcttga ttctgaaact
    13741 gcaacttttc aaggagcttg tggagtagct ttccgattgc tagagcctgt ggaagaagat
    13801 gatggttgga agttaagagc aagtaatatt gaaccttgga gagcagaaag atatggaaat
    13861 gccggaatct atatcaaaga aaagtatgac tcttaccaga aaaaattttt tcaagaaatg
    13921 aaagttatga caagaaataa aattctgaca tatgcttgct atggtgattt gaattttgtt
    13981 acaagcggaa cgttcaaaaa aattgatgag accgataacc ctttggggac gattaccttg
    14041 gcagaattta aaaataatac gaatcgttat tgtgattttg aagtagctga ggaaattgga
    14101 gatgcaattg atcgtgcttt gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg
    14161 tacatgcttg ttactggcac aacaatgagc aaaggcactg ctaaagaaat gatgaatcag
    14221 ctagggatta tcaacttaaa agcccctact gcaaaagctg aatatgtcac aaaaaattta
    14281 gcaaaagatt tcaatgaata tcatatggat ctattgaaaa aacagtttta caccatttgt
    14341 aaagcaatcg attttaacga tgaggtgttt aaatcaaaca gttctggaga agctcgcaag
    14401 tggcaaatca ttagcctaga agcgaaaaca aatactaaag agcaatattt tagagaagga
    14461 ttgaaagaat gcgcagaaac aattgctgct tttcttaagt ttcatgacaa agtagaaatt
    14521 gagcctgaaa aaattatttt tactttctct agatctttgc caactgattt aagttattta
    14581 gctgaagctt tacctaaact tgcaccatac gtatccaaac gaactattca gagccaaatt
    14641 ccatttgtaa cagatgttga ttatgaaaat gaaatgatgg aattggaaag tggaagtgct
    14701 tatccagata gtgaatacaa ttttggcgga ggtggcaata gtgacgatag aaacgaagta
    14761 ttggaccaaa cgtcgagaac tggaggatca agcaaggctc aaacaagaaa atcagacact
    14821 taaaaaatta actagtgtat ttcctgaagc actgaaagag atacaagcaa aactattatc
    14881 acaagctgac ttacacaata tcacttatcc agaaatgatg gagttttaca gtacaagtaa
    14941 tcagaaaaaa tatcgtgaat atgtggaaaa aaattataag tcattaaaaa tgtatgatgc
    15001 aaaatacaaa gagtttatcg atgaattttt tccaccattt gactatgcaa aagtcaatcg
    15061 cttattacaa atacgatcag atgtatttaa aattcttgca gaatatgcga tggatgcaga
    15121 tgtgaatcaa tatttttctg atcgcttaga ggaaattctt caaagaacat attcttctaa
    15181 tgctaatgtt tttgttcaac ttttaaacgt tgatatacca aattatttac cagaaaatga
    15241 aattaaacat tatttgaact atccatggtc aggaaagaca ttttcaagaa gactttgggg
    15301 aaatatttca tcgcttgaac agaaactctc taatgctatt gtaaaaagtg ttgctagtgg
    15361 agaaggcgtt atacacgcat taaacaccat gagattagac tcagaaattt gtgacatgtt
    15421 taagttagaa gaatcaaagt ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc
    15481 aaaatttgca caagatggta ttgaaaaatc atatttagaa acaggtattg aggaatacaa
    15541 cgtattgact gcaaaagatg agagagtttg ccggatttgt ggaggaaagg caagtaagaa
    15601 tccctataaa ctgaaagatg ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg
    15661 tagatgtacg gatgttccta atttaccaaa attaggaaag gatattgatg aagaatatga
    15721 ccgtttattt ggcgatttat tagatgagtt tgcacatgat tcttttggaa ttaatttgaa
    15781 acggaggaag tagaatgaaa gatttttttg aagcagtact aacaattaat gtaaatgctg
    15841 atattgcaga agcctacaaa acagctattg agtctgagaa ccatcctaat ggcttgagag
    15901 accattggaa tggcaattat gcctacgtgg ttattggcga tcaaactgtt aattatcaag
    15961 ataatactcc agttgataag aataccgtta atttaacgat tcaattatta tctcattcat
    16021 taccaaattt aaaagaaaca gttgattggt atgaaaagat gggatgtatt gttgttagaa
    16081 ctgactacaa agaaggaaag tctagtaatt aggctttttt attttgtccg aaatgacgtt
    16141 aaactagcgc aatactgggc ttggttgaat ggtggggcgc aactattaaa actcaaagca
    16201 atgcggggcg tgaaaacgaa tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat
    16261 taatgccaat gaatttgcaa tactttgccg aaggtgacga tcaaaagttt tcttttgatg
    16321 acttcaaatc ttttgtggaa tccaatgaag aagctcaaaa atttattcag tcacaatctc
    16381 aaagtgttgc cgacaaacaa ttggaagctt ggaaacaaaa taacttagat aaaattaaac
    16441 aggataccat caaggaatat gaggaatcta agaaaaacaa gtcacctgag caaattcaac
    16501 tggaaaaact acaagctgaa tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata
    16561 aagcatttgt tgcagaacaa attgctggat tagaactaga tggagagtta aaagagtcta
    16621 tttctcaatt tatgctaaat aatcttgtta gttcggatac agatttcact aagaatgctg
    16681 ttgaaggttt tacaagtgtt ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag
    16741 aactacaaat gaagtctgca tttggtggaa ctcaacaatc gaataaccag gttcagcaga
    16801 acaatgaaac attaacaaat ccagaagaac aattaggaca aattcttcaa caatttaact
    16861 aggagagtga aaaattatga aaaaaacatc tttaaataat ttagagtatt tggatatttc
    16921 accagcgatt aatgctatgc aagtaccaaa tacacctttt ttaagctatt tacttggtgc
    16981 tggaaaaaca gagccagcaa actcgacaga aattaaatgg cgtgaatatg atatcaacaa
    17041 cgatgattct tctgaaaaac ttgagggcgg agaatatcca gatgctgaat caggtcgaac
    17101 ttggtttaac aactatactg aaatttttag aaaatcaacc tctgtatctg gtacattaga
    17161 tgctattaat gtgaatggag tcggaaatga attaactaat caagtagcac tacgtggtat
    17221 ggaaatgaaa attgatttga accgaaaatt gattactggt gtaaaagctg atgaaaatgg
    17281 ttctaaaggt cgtcgaatga atgggatttt gaacttgatc aattcagcaa ataaggcaga
    17341 aacagccact gcgggtgcag taacaagaaa agatatcgat actttattta aattgatgta
    17401 tgaaaaaggt tatatgggag aaaaactatg cttgatttct ccagatatgc aggagttaat
    17461 gactgatgag ttagatggaa aatcaacaaa aattgttcag ttcggggaaa gagtaacttt
    17521 tggattgcaa attggaaata tcgtgtctaa ttacggtaca ggtattgctc tactagaacc
    17581 atcattgcca aaaggaacaa ttgccgcaat cgatactaat tatgtgaaac tacgtccatt
    17641 acgtgaatgg agagcagaag aacttgcaaa aacaacggat tctagacgta tcggtcttgt
    17701 aggtgaatac tctcttgaat acaatgcttc aaactctggg gcaattttaa atttaaagtc
    17761 tgaataaaag ggagttagta ctcccttttt tgataggagg aattacagtg gtaaaaaaag
    17821 atgaaactaa aaaagatgaa gtcgtgaaat atagagtagg taaaactaaa aattttgttg
    17881 ggtttgttca tcctaaaact cgtagattta tcacagcaga ttcaaataac gaatttatca
    17941 tttctataga tgataaagaa gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa
    18001 tttaggaagt gatctgatgg atgaatcgct aaaaacggaa atcattgagt ctacaaaaga
    18061 agattttcca gatttgagtg aagaacgcat aactaattta ttagaaataa ttttgctaga
    18121 aattgaatca tacaacactt gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat
    18181 tagtgaagtg ttgtataaaa taataaaaaa tgaatcagaa aaaacagtat cttcagttag
    18241 acgtggcgat acaacgatta gttatgcttc aacaacaaat gatgttagtg agttacttct
    18301 aggctatggt gatttgatac gaagagttat tggttgtgga ggattggagt ttttttaatg
    18361 aatgaagcag atattttaga aactacttat gaagatagtt gtattattga aagactgacg
    18421 gacattgaag attctaatac aaatattact attcaagatt acaaaaaagt atatgataat
    18481 cctatttctt gcgctctttc acaaggtcaa atcgatggac tagcagtcat agaagatgga
    18541 gagatggtaa atgtttcaac tgacacatat aaattatttg ttcatcctaa gattaaacta
    18601 aaaaaaggag atcgaataac aataactcaa aaggccagtg gcttaatttt ttctctattt
    18661 gctactaagc ctttttacta tcctagtcat tgtgaagtga atttgatagg aagtgagaaa
    18721 aatgggtaat ctcaaatatg aatcaaatgc agaaaagatc attgagaatt ttaaaaatat
    18781 gactgtaatt gctcaaaaag aaggaatatc cttcgttaac gattcaatga ataaagtcgt
    18841 tagtttagct aaaccattaa ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt
    18901 agtaaaagct agaaaactat caagcggtcg aattgttgga gaagttataa acaatgaaca
    18961 ttattttaaa tatgtagaag aaggtcatag gactaaaaat ggtggctttg tcaaagggaa
    19021 attcatgtta actcgtgcaa cgaatcttgc aaatatgtct tatattcctc gaagatttaa
    19081 acaaatggca ataaaaatcg ttaagaaagg aaagtaacat gaaagatgaa atcattgctg
    19141 caatcagtag caaattaaaa gaaatctatc ctgatgggac aatatatctt gattcagtta
    19201 tgcagtcaac taaagatttt tatttcgttt tatcagtaat ggaatctgga actgaaaatg
    19261 taggaattga tattcaaaat gtttctttct taattgatat tgcattgatt gataataacc
    19321 ctaatagaaa tttaattaat gaattagtct cacgttgtgg gacttttttt aatacgatta
    19381 caatcgatga gcaaatatta tttccaaaag cctatttacc tgatgaagca gatggtgttc
    19441 aacatattcg ttttacatta gaatttccac aatatattga atggagtgaa agataaatgg
    19501 gagaaaaaag aagtaaaacc ggaattattt ctgtcgaaaa acctacttgg tttcctttgg
    19561 aagatgagac agggaatttt ccggtatatg gtactgcgtt gccaatggga acagcagtaa
    19621 gtatcaaacc aacagctaat tatgaaacaa cacaagatta tggggattca gtggttcaag
    19681 atcaatttac agcttttggt ggtgcagaag ttgaactgga agcaaatgga tatactcata
    19741 aagttttatc tacaattact ggagggaaaa ttgttaaagg tggtgccttg cgttctggag
    19801 aagatattgc gcaagatggt gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc
    19861 gttatacggt cttttataaa ggacagtttg ctctagattc agatgaaaca tcaacgattg
    19921 aaggaagtaa agtaagtttt actcatccaa cttggaaggg ttcatttgta gatgtaccag
    19981 gacttggata tatgtattca gttgatgaag atgatgaagg cgtggataaa gcaatgatcg
    20041 gaaattggtt tacaaaagta gcaattccaa ttgaagaagc agaactttca ggaggtacag
    20101 aataatgtca aaatatcaaa caacgattaa attaacgaaa aaagacgaag aaggtaaata
    20161 tgagcaagta caatttaaat ctgctgaatt tttaccagga acagttgtag aagatgctgc
    20221 aggagttatg gaagaaatgc aaacagcgac tgataagcaa tcagttaaaa aagctttaag
    20281 tcgtgcttat tcatttattg cagatacttt atttgaggga caatttactg gtgaagatta
    20341 ttgcaaagga attgatgctc gtgagattgc ctcattgaca ggaaaattat tgaagtctgt
    20401 tactgcaggt tttgatgaaa cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag
    20461 tcaccttcat ttaagtattc gattacttac cgagaattag atataaagac gcaattactc
    20521 gaagcaggtt ggacgttacc agaaattgaa cgtactgact tggatgagtt aatgcgtctt
    20581 tttgctttta gagatgcggt aaaagagcat gaagatgttg agtactatga taatttcact
    20641 caattttagg aggtgatact ttgaataacg atgacctaat tctgaagatg atactagatg
    20701 aatctggttt tactgccggt atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa
    20761 caatcgaaag aaaaagtaga aatagtggca gttcattagg tagtatttgg aaaatatttg
    20821 ctggaagttt tctagctagc ggagtaacta gaatcgtagg agctggcttt gatctaatca
    20881 agggttccat aagtggagcg gttgatcgag tagatacgat gaataacgct ctacgaaatt
    20941 tccaaaatat gggatttagt aattcagaaa ttatgaagaa tatcggaaag aatgggcttt
    21001 tatctcaagg tattcaagga cttcctactg ctttgaatga tgcgataagt catgttcaac
    21061 ttcttgcttc ttctacaggt gatttaactc gttcaactca aatatttaaa gctttaaatg
    21121 atggaattct tggtttcggt ggttcaactg atcaagttaa tgaagctgtt attcaattat
    21181 ctcaaagttt ctcaaacgga aaagtagacg cacaaacttg gaactcaatg atcaacgctc
    21241 aacttggacc tactctttct gctattgcta aaaagatggg aattacaatg ggagatctga
    21301 aagaaggttt atctcaaggt aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa
    21361 tggataccaa aggtggcgga ggacttaaat cattaagtca aatcgctaaa gattctacta
    21421 agggaattaa aacctctata caaaatgcaa aaacagctgt tacacgtggt gttggtgagg
    21481 taatagaagg attaaataaa gcattagtag attcagattt aggtggattt aagggaatta
    21541 ttgataaagt gggcagttca atggaatcgt tcttaaaagt aattgctgca aatattccta
    21601 cagcagtatc atttttaagt aatttattta acgaaattca aaaatttggt tctgctttga
    21661 aattcatgat gccctttctt gttcctgcag ctaccgcttt tggagcgttc atgtttcaac
    21721 ttaaaggtat accagcaata ataaaaagct tcaataattt taagaatgcc ataattggcg
    21781 ttggaaattc attaaagatt atgggggcaa tagctgctgc taatccattt gttttaatta
    21841 ttggtgcagt cgttggtgca atagctgttt tcggttactt tatggcaacc aatgaagagc
    21901 ttagaaacaa ggttatatct atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa
    21961 aaaatataaa ggactgggga attgatactt ggaattctgc taaagaaatg gcatcaaatg
    22021 cagttgaggg tgtcaaagat gcttggtcag gaacaaagga atggttttct aacacctgga
    22081 aaggtattaa ggatggggcc acaggtttat ttgataaaac agtagagact tcaagaaatg
    22141 cagtcgatag tgtaaaaaat gcatggtcta atatgaagaa atggttttct gatacttggc
    22201 aaagtataaa agattcagcc agagaaaaat ggaatggaat caaaggttct attatgtcag
    22261 ttgctggtcc attgattaca ggtataaaga atgcattttt acatgttact ttttatctag
    22321 agaccttatg gaataattta gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg
    22381 ttatacttgc gccggttttg tttattacct ccatgatttc tggtggatgg gaagaaacaa
    22441 aaaataatat gattg
//
LOCUS       DAZKSR010000051        21972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000051 DAZKSR010000000
VERSION     DAZKSR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.051"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(369..995)
                     /locus_tag="ACG8XG_001686"
     CDS             complement(369..995)
                     /locus_tag="ACG8XG_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773606.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="ACG8XG_001687"
     CDS             complement(1290..1625)
                     /locus_tag="ACG8XG_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0773607.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="ACG8XG_001688"
     CDS             complement(1612..1896)
                     /locus_tag="ACG8XG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0773608.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="ACG8XG_001689"
     CDS             complement(1952..3475)
                     /locus_tag="ACG8XG_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0773609.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="ACG8XG_001690"
     CDS             complement(3468..4643)
                     /locus_tag="ACG8XG_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0773610.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="ACG8XG_001691"
     CDS             complement(4647..4832)
                     /locus_tag="ACG8XG_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773611.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="ACG8XG_001692"
     CDS             complement(4798..6492)
                     /locus_tag="ACG8XG_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0773612.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="ACG8XG_001693"
     CDS             complement(6489..6962)
                     /locus_tag="ACG8XG_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0773613.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="ACG8XG_001694"
     CDS             complement(7031..7186)
                     /locus_tag="ACG8XG_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFQ0773614.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="ACG8XG_001695"
     CDS             complement(7320..7700)
                     /locus_tag="ACG8XG_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0773615.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="ACG8XG_001696"
     CDS             complement(7704..7919)
                     /locus_tag="ACG8XG_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773616.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="ACG8XG_001697"
     CDS             complement(7922..8329)
                     /locus_tag="ACG8XG_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFQ0773617.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="ACG8XG_001698"
     CDS             complement(8595..10070)
                     /locus_tag="ACG8XG_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFQ0773618.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="ACG8XG_001699"
     CDS             complement(10060..10908)
                     /locus_tag="ACG8XG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFQ0773619.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="ACG8XG_001700"
     CDS             complement(10945..11307)
                     /locus_tag="ACG8XG_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773620.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="ACG8XG_001701"
     CDS             complement(11308..11466)
                     /locus_tag="ACG8XG_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773621.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="ACG8XG_001702"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="ACG8XG_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="ACG8XG_001703"
     CDS             complement(12228..12539)
                     /locus_tag="ACG8XG_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773622.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="ACG8XG_001704"
     CDS             complement(12583..12876)
                     /locus_tag="ACG8XG_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773623.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="ACG8XG_001705"
     CDS             13070..13717
                     /locus_tag="ACG8XG_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773624.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="ACG8XG_001706"
     CDS             13778..14923
                     /locus_tag="ACG8XG_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0773625.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="ACG8XG_001707"
     CDS             complement(15004..15309)
                     /locus_tag="ACG8XG_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0773626.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="ACG8XG_001708"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="ACG8XG_001708"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0773627.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="ACG8XG_001709"
     CDS             complement(15762..16028)
                     /locus_tag="ACG8XG_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0773628.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="ACG8XG_001710"
     CDS             complement(16283..17008)
                     /locus_tag="ACG8XG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0773629.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17369..17439)
                     /locus_tag="ACG8XG_001711"
     tRNA            complement(17369..17439)
                     /locus_tag="ACG8XG_001711"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17405..17407),aa:Gly,seq:ccc)
     gene            17556..18308
                     /locus_tag="ACG8XG_001712"
     CDS             17556..18308
                     /locus_tag="ACG8XG_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0773630.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18324..18803
                     /locus_tag="ACG8XG_001713"
     CDS             18324..18803
                     /locus_tag="ACG8XG_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0773631.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19054..20634)
                     /locus_tag="ACG8XG_001714"
     CDS             complement(19054..20634)
                     /locus_tag="ACG8XG_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0773632.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20627..21370)
                     /locus_tag="ACG8XG_001715"
     CDS             complement(20627..21370)
                     /locus_tag="ACG8XG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773633.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21546..>21972)
                     /locus_tag="ACG8XG_001716"
     CDS             complement(21546..>21972)
                     /locus_tag="ACG8XG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HFQ0773634.1"
                     /translation="YEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYD
                     EILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITS
                     ISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         6418 a         4447 c         3578 g         7529 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggacgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    17161 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    17221 tacactgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    17281 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    17341 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    17401 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17461 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17521 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17581 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17641 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17701 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17761 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17821 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17881 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17941 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    18001 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    18061 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    18121 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    18181 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    18241 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    18301 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    18361 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18421 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18481 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18541 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18601 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18661 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18721 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18781 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18841 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18901 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18961 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    19021 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    19081 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    19141 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    19201 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    19261 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    19321 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    19381 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19441 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19501 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19561 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19621 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19681 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19741 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19801 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19861 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19921 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19981 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    20041 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    20101 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    20161 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    20221 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    20281 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    20341 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    20401 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20461 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20521 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20581 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20641 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20701 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20761 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20821 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20881 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20941 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    21001 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    21061 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    21121 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    21181 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    21241 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    21301 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    21361 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21421 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21481 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21541 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21601 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21661 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21721 tcatattcga gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct
    21781 ttcaatacta ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca
    21841 tcgtaatgga tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta
    21901 gcatgttgaa caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta
    21961 tttttttcat aa
//
LOCUS       DAZKSR010000052        21498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000052 DAZKSR010000000
VERSION     DAZKSR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.052"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..882
                     /locus_tag="ACG8XG_001717"
     CDS             181..882
                     /locus_tag="ACG8XG_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0773635.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1491..2507)
                     /gene="tsaD"
                     /locus_tag="ACG8XG_001718"
     CDS             complement(1491..2507)
                     /gene="tsaD"
                     /locus_tag="ACG8XG_001718"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0773636.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2504..2968)
                     /gene="rimI"
                     /locus_tag="ACG8XG_001719"
     CDS             complement(2504..2968)
                     /gene="rimI"
                     /locus_tag="ACG8XG_001719"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0773637.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2975..3517)
                     /gene="rimI"
                     /locus_tag="ACG8XG_001720"
     CDS             complement(2975..3517)
                     /gene="rimI"
                     /locus_tag="ACG8XG_001720"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0773638.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3501..4223)
                     /gene="tsaB"
                     /locus_tag="ACG8XG_001721"
     CDS             complement(3501..4223)
                     /gene="tsaB"
                     /locus_tag="ACG8XG_001721"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0773639.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4414..5394)
                     /locus_tag="ACG8XG_001722"
     CDS             complement(4414..5394)
                     /locus_tag="ACG8XG_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0773640.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5418..6902)
                     /gene="galT"
                     /locus_tag="ACG8XG_001723"
     CDS             complement(5418..6902)
                     /gene="galT"
                     /locus_tag="ACG8XG_001723"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0773641.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6914..7903)
                     /gene="galE"
                     /locus_tag="ACG8XG_001724"
     CDS             complement(6914..7903)
                     /gene="galE"
                     /locus_tag="ACG8XG_001724"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0773642.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8151..8318
                     /locus_tag="ACG8XG_001725"
     CDS             8151..8318
                     /locus_tag="ACG8XG_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0773643.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8380..10191)
                     /locus_tag="ACG8XG_001726"
     CDS             complement(8380..10191)
                     /locus_tag="ACG8XG_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0773644.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10188..10553)
                     /locus_tag="ACG8XG_001727"
     CDS             complement(10188..10553)
                     /locus_tag="ACG8XG_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0773645.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10716..11111)
                     /locus_tag="ACG8XG_001728"
     CDS             complement(10716..11111)
                     /locus_tag="ACG8XG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0773646.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11129..12091)
                     /locus_tag="ACG8XG_001729"
     CDS             complement(11129..12091)
                     /locus_tag="ACG8XG_001729"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0773647.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12091..12303)
                     /locus_tag="ACG8XG_001730"
     CDS             complement(12091..12303)
                     /locus_tag="ACG8XG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0773648.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12324..13022)
                     /locus_tag="ACG8XG_001731"
     CDS             complement(12324..13022)
                     /locus_tag="ACG8XG_001731"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0773649.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13042..13584)
                     /locus_tag="ACG8XG_001732"
     CDS             complement(13042..13584)
                     /locus_tag="ACG8XG_001732"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0773650.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13716..14720)
                     /locus_tag="ACG8XG_001733"
     CDS             complement(13716..14720)
                     /locus_tag="ACG8XG_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0773651.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14717..15706)
                     /locus_tag="ACG8XG_001734"
     CDS             complement(14717..15706)
                     /locus_tag="ACG8XG_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0773652.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15703..16509)
                     /locus_tag="ACG8XG_001735"
     CDS             complement(15703..16509)
                     /locus_tag="ACG8XG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773653.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16675..17631
                     /locus_tag="ACG8XG_001736"
     CDS             16675..17631
                     /locus_tag="ACG8XG_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0773654.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17708..18226)
                     /locus_tag="ACG8XG_001737"
     CDS             complement(17708..18226)
                     /locus_tag="ACG8XG_001737"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0773655.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18314..18463)
                     /gene="rpmG"
                     /locus_tag="ACG8XG_001738"
     CDS             complement(18314..18463)
                     /gene="rpmG"
                     /locus_tag="ACG8XG_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0773656.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18691..19137
                     /locus_tag="ACG8XG_001739"
     CDS             18691..19137
                     /locus_tag="ACG8XG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0773657.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19332..21227)
                     /gene="asnB"
                     /locus_tag="ACG8XG_001740"
     CDS             complement(19332..21227)
                     /gene="asnB"
                     /locus_tag="ACG8XG_001740"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0773658.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6475 a         4560 c         3700 g         6763 t
ORIGIN      
        1 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
       61 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
      121 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
      181 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
      241 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
      301 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
      361 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
      421 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
      481 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
      541 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
      601 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
      661 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
      721 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
      781 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
      841 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
      901 gtgaccctga taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc
      961 cggcagtttt agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta
     1021 atattcactg tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc
     1081 cgatctagat tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag
     1141 gaacggataa tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta
     1201 tgtaaaaaat agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt
     1261 acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga
     1321 aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt
     1381 tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt
     1441 tcctgggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat
     1501 aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc
     1561 acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc
     1621 actagttcta cctcagggag tttagctgcc aaagcatcct gcaatccttc acgcaaccct
     1681 tgattggccg ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct
     1741 cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca
     1801 tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt
     1861 tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg
     1921 taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga
     1981 cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca
     2041 atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca
     2101 agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct
     2161 gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca
     2221 gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat
     2281 aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt
     2341 tctacgtgat ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt
     2401 atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc
     2461 gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg
     2521 tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc
     2581 gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata
     2641 gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta
     2701 acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct
     2761 cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta
     2821 aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag
     2881 gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt
     2941 catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa agttttatca
     3001 tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa
     3061 accccaattt ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca
     3121 tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga
     3181 ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga
     3241 acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat
     3301 gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat
     3361 tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta
     3421 gtacgtaacg acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa
     3481 aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc
     3541 gtctccaacc atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt
     3601 ttctctgcaa ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata
     3661 tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag
     3721 aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg
     3781 aacaaatcag aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc
     3841 cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata
     3901 ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct
     3961 ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa
     4021 gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct
     4081 gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt
     4141 tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca
     4201 gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc
     4261 attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa
     4321 tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg
     4381 tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa
     4441 ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat
     4501 cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg
     4561 cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac
     4621 cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc
     4681 aaaaagcgca tcaggaacat gcggattcgt ttttaaatat tgcttcactg cttgatatcc
     4741 tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa
     4801 aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata
     4861 ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac
     4921 tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc
     4981 agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc
     5041 gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc
     5101 tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa
     5161 ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg
     5221 cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa
     5281 aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga
     5341 aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct
     5401 ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt
     5461 tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat
     5521 ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt
     5581 tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct
     5641 acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc
     5701 aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca
     5761 ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc
     5821 cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg
     5881 atagatactg tttcatccga atatgctttc catttttcca atactgagtt agctgcttca
     5941 ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact
     6001 cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc
     6061 ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca
     6121 attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc
     6181 agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc
     6241 gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga
     6301 ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct
     6361 tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct
     6421 gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca
     6481 tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca
     6541 tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc
     6601 tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct
     6661 tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg
     6721 acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct
     6781 agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc
     6841 atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac
     6901 atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg
     6961 ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt
     7021 tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg
     7081 gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt
     7141 tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta
     7201 aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg
     7261 gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta
     7321 aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt
     7381 ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac
     7441 accacttcat cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag
     7501 ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag
     7561 agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt
     7621 atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg
     7681 cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt
     7741 tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg
     7801 tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt
     7861 gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga
     7921 ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt
     7981 gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag
     8041 taaacatttt caagggtttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt
     8101 tctcttttgt tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat
     8161 ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggttg
     8221 cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa
     8281 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
     8341 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
     8401 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
     8461 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
     8521 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
     8581 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
     8641 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
     8701 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
     8761 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
     8821 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
     8881 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
     8941 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
     9001 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
     9061 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
     9121 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
     9181 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
     9241 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
     9301 caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
     9361 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
     9421 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
     9481 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
     9541 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
     9601 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
     9661 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
     9721 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
     9781 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
     9841 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
     9901 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
     9961 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
    10021 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
    10081 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
    10141 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
    10201 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
    10261 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
    10321 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
    10381 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
    10441 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
    10501 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
    10561 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
    10621 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
    10681 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
    10741 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
    10801 aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg
    10861 tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct
    10921 aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc
    10981 attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt
    11041 agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc
    11101 cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac
    11161 caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt
    11221 gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg
    11281 ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc
    11341 aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt
    11401 cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt
    11461 tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg
    11521 tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag
    11581 gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc
    11641 cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat
    11701 cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga
    11761 tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc
    11821 agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac
    11881 ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga
    11941 ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc
    12001 tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac
    12061 ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc
    12121 aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa
    12181 acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc
    12241 acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc
    12301 catctttaag cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa
    12361 atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa
    12421 tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc
    12481 ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac
    12541 acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga
    12601 aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc accaccagac
    12661 agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata
    12721 taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac
    12781 agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact
    12841 aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg
    12901 atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa
    12961 gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc
    13021 atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct
    13081 ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg
    13141 aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct
    13201 attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc
    13261 tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc
    13321 ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt
    13381 ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc
    13441 tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc
    13501 tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg
    13561 taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg
    13621 aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat aaaaagaaga
    13681 cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc gtgtttttgt
    13741 ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta
    13801 ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag cagaaaaatg
    13861 gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac gactagcttc
    13921 gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc tacactaccg
    13981 cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac gttaaggcct
    14041 aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc ataaagtcct
    14101 cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg ccagctcgca
    14161 ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt agataattgg
    14221 atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc ggacaataag
    14281 attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac acagcaagca
    14341 gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga atagtgcgaa
    14401 aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc caatatacta
    14461 gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac gccaagacaa
    14521 gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac aatcagcgcc
    14581 aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta aataaaaggc
    14641 gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa gaaatagaac
    14701 gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa aacggaacgc
    14761 caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg
    14821 taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt agaacataac
    14881 gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca ataaatgaaa
    14941 caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag aaaacaatca
    15001 accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc aaatgaatca
    15061 aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca
    15121 atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa aaagcaagat
    15181 cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa aaagaactaa
    15241 tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa aactgcaaaa
    15301 aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga aacacagaaa
    15361 aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa gcacccgcgt
    15421 tgattcctag taagcctgaa tctgctaaag gatttcttgt cactccttgc atcaatgcac
    15481 cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc aaacgaatat
    15541 ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat
    15601 ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa
    15661 caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt gatcctcctg
    15721 ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg gaagcgtgac
    15781 aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca cttcttcagg
    15841 tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg aaaaacgtga
    15901 agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt gattgatttc
    15961 agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg tcggttcatc
    16021 caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac gctgggtttg
    16081 tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg tagcatccaa
    16141 cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt tttgataagg
    16201 gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt cactagtttg
    16261 tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga tatcagagtt
    16321 attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt tcaataaagt
    16381 agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag gaatcgtaac
    16441 agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag ctgctcttaa
    16501 tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg ctgtttcaac
    16561 atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca gcgttcgaaa
    16621 tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa
    16681 ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt actgagcgct
    16741 tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac ttcatccaat
    16801 agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa cgcgcccaaa
    16861 cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa acctatcgcc
    16921 cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt aaaggatgtc
    16981 ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc tgatttgctg
    17041 ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg
    17101 ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact aaaagatgtc
    17161 ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc
    17221 atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc tgcagtgttg
    17281 tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta
    17341 tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa aaaagccact
    17401 tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga ctacatcatc
    17461 cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa gaatctaaaa
    17521 gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct atataacgga
    17581 cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg attttaacta
    17641 aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt cttattaaca aactagacgc
    17701 atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat ttttgaaaac
    17761 gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata agggcgaagt
    17821 ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg atcaatgttc
    17881 gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca cccgaaacat
    17941 agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa tgcattactt
    18001 ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata tggcgataag
    18061 gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa attcgatcca
    18121 ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac aaaccatttt
    18181 tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta tccgctcctt
    18241 atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca aggaattgag
    18301 aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat
    18361 atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt agatagttac
    18421 gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc cctcccattt
    18481 ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat tttttctaga
    18541 ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat acaacggttt
    18601 tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg tttatataga
    18661 ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga ctttcctgca
    18721 tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta
    18781 ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca agcaaaaaaa
    18841 tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc
    18901 aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc agatgtgatc
    18961 gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac
    19021 cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa
    19081 tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga ttattgaaca
    19141 tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg
    19201 ttcctctgcc aactccgaac cataagtcat aatatctata aaacgggaag agctgttttt
    19261 tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt tttattgata
    19321 tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat cgtttcatct
    19381 acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt tctttgctct
    19441 tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg atcaaaaaat
    19501 tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg ttcttctttc
    19561 aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca ttcttccgga
    19621 agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt caataaaaac
    19681 ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg tactcggact
    19741 tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt tggcatccat
    19801 tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc tgttttctcg
    19861 taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg tgttaacact
    19921 tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc ttttgcagga
    19981 gcaagtgatt catataaatg gatccgtcca tggaagtttt tcatttttcc gattttacgt
    20041 cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat cacacggatg
    20101 aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc atccgcccct
    20161 tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt
    20221 acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg gaatgctttg
    20281 aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag ttcggttagt
    20341 tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga gaaagaatga
    20401 tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa gaaagaacca
    20461 acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc gtcgattttt
    20521 tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc ccaatattgc
    20581 tgaatgtcta tttttccatc tttatacgta taataatggc cttcaggcaa acgatagaca
    20641 tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata aggtttcaac
    20701 gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt gatttctgaa
    20761 ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc aaagtggtct
    20821 cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa cattccgcgg
    20881 atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac ttctgtatct
    20941 gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg ctggtaatta
    21001 tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat aggctgagta
    21061 ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg tttatcgata
    21121 tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt attaataatt
    21181 gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta actttcacat
    21241 cctaatataa aattactgtt tatttcattc atatgcatct tatcatagaa taaatagcgg
    21301 acgaatattt tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt
    21361 ttacagaaaa taaagtccca atatctaatt taccatgttt tcttttttga ggctacctta
    21421 ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc aaaaaaacca gcaaaaaaag
    21481 acacgaccta aagatcgt
//
LOCUS       DAZKSR010000053        21243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000053 DAZKSR010000000
VERSION     DAZKSR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.053"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..661)
                     /locus_tag="ACG8XG_001741"
     CDS             complement(50..661)
                     /locus_tag="ACG8XG_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0773659.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(784..909)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(991..1707)
                     /locus_tag="ACG8XG_001742"
     CDS             complement(991..1707)
                     /locus_tag="ACG8XG_001742"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0773660.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1713..2282)
                     /gene="scpB"
                     /locus_tag="ACG8XG_001743"
     CDS             complement(1713..2282)
                     /gene="scpB"
                     /locus_tag="ACG8XG_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0773661.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2266..3036)
                     /locus_tag="ACG8XG_001744"
     CDS             complement(2266..3036)
                     /locus_tag="ACG8XG_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0773662.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3033..3404)
                     /locus_tag="ACG8XG_001745"
     CDS             complement(3033..3404)
                     /locus_tag="ACG8XG_001745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0773663.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3423..4310)
                     /gene="xerD"
                     /locus_tag="ACG8XG_001746"
     CDS             complement(3423..4310)
                     /gene="xerD"
                     /locus_tag="ACG8XG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0773664.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4406..4879)
                     /locus_tag="ACG8XG_001747"
     CDS             complement(4406..4879)
                     /locus_tag="ACG8XG_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0773665.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4984..5847)
                     /locus_tag="ACG8XG_001748"
     CDS             complement(4984..5847)
                     /locus_tag="ACG8XG_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0773666.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6043..8142)
                     /locus_tag="ACG8XG_001749"
     CDS             complement(6043..8142)
                     /locus_tag="ACG8XG_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0773667.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8362..9471)
                     /gene="rpoD"
                     /locus_tag="ACG8XG_001750"
     CDS             complement(8362..9471)
                     /gene="rpoD"
                     /locus_tag="ACG8XG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0773668.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9495..11336)
                     /gene="dnaG"
                     /locus_tag="ACG8XG_001751"
     CDS             complement(9495..11336)
                     /gene="dnaG"
                     /locus_tag="ACG8XG_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0773669.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11518..13833)
                     /locus_tag="ACG8XG_001752"
     CDS             complement(11518..13833)
                     /locus_tag="ACG8XG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0773670.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14018..14878)
                     /locus_tag="ACG8XG_001753"
     CDS             complement(14018..14878)
                     /locus_tag="ACG8XG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0773671.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14937..15569)
                     /locus_tag="ACG8XG_001754"
                     /pseudo
     CDS             complement(14937..15569)
                     /locus_tag="ACG8XG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15736..16497
                     /locus_tag="ACG8XG_001755"
     CDS             15736..16497
                     /locus_tag="ACG8XG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0773672.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16523..19114
                     /gene="mprF"
                     /locus_tag="ACG8XG_001756"
     CDS             16523..19114
                     /gene="mprF"
                     /locus_tag="ACG8XG_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0773673.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19302..20489)
                     /gene="tuf"
                     /locus_tag="ACG8XG_001757"
     CDS             complement(19302..20489)
                     /gene="tuf"
                     /locus_tag="ACG8XG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0773674.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20857..21213
                     /locus_tag="ACG8XG_001758"
     CDS             20857..21213
                     /locus_tag="ACG8XG_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0773675.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5818 a         4458 c         3720 g         7247 t
ORIGIN      
        1 atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg
       61 ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat
      121 gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc
      181 caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa
      241 acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag
      301 cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac
      361 taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc
      421 ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg
      481 ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac
      541 taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa
      601 ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca
      661 tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg
      721 aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat
      781 aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta
      841 tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg
      901 aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg
      961 caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac
     1021 ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc
     1081 gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac
     1141 ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc
     1201 cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt
     1261 tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt
     1321 atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag
     1381 caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat
     1441 cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc
     1501 tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac
     1561 ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac
     1621 ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg
     1681 ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag
     1741 aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt
     1801 tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc
     1861 ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc
     1921 tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc
     1981 tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc
     2041 ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg
     2101 ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat
     2161 tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca
     2221 ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc
     2281 atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg
     2341 cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc
     2401 acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat
     2461 acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt
     2521 ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc
     2581 gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt
     2641 cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc
     2701 tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc
     2761 ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt
     2821 gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt
     2881 tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata
     2941 tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac
     3001 atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc
     3061 cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt
     3121 tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa
     3181 gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga
     3241 atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac
     3301 gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt
     3361 cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat
     3421 gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt
     3481 gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa
     3541 tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact
     3601 tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga
     3661 gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt
     3721 aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga
     3781 atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga
     3841 tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc
     3901 aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt
     3961 tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata
     4021 tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg
     4081 agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc
     4141 agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt
     4201 agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat
     4261 ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc
     4321 ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg
     4381 agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct ttttcatagc
     4441 attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa tgataacgtt
     4501 cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc
     4561 agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc aaatcataac
     4621 gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc aagatttcaa
     4681 gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact aaaaagaaga
     4741 tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga gtagcttctc
     4801 tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt ttcatcgccg
     4861 aaatagcatt catgaccatt tactcaaccc caacaattta taataattac aaataaaaaa
     4921 agatataaca ctatgttaat taaaattatt ataagctagt taagaaaaac atgcaaggga
     4981 ttctcaatta gattttttga ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg
     5041 tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg ctgataccaa aagctttcat
     5101 gatttcttct ggatccgatt tatctgtaaa gttgatttgc tgatctgcag aacgttctaa
     5161 aatggttaaa atcatttgtg catcatctga tatagcttca tatgctcttg gcttcaatga
     5221 aagattcagt acaccgtctg gtcggacacc aatcactcga ccttttatct gctcgcctag
     5281 acgaggttca ttatatcgtt cagaaggatg gataaaccca agatagaaat cttccgttaa
     5341 aacgtacgta cctgttaatt tcaagcggta tgctgttcct atcacatctt tgtttttcat
     5401 ttcttctgtt ccatatttgc ttaaagattt gaatattttt tcatctgcta aactgcccca
     5461 aatacggtct tttgcatcaa ctctaaggga aatcatcaag cgatcccctt ttttaggcca
     5521 tagctctgtc attgtcggca attcatcaag agatacgaca aagtctttat ctggaagacc
     5581 gatatcgaca aacgcaccta aatcttttcg tgtagcagta acgattccaa aagcgtaatg
     5641 accgatacgg cttttaggaa tttgtgtagt gatccgattt tcatgttttt gattttggta
     5701 accgaatcct tcaactgctt caccgatttt gtgttcacct tctgatttgc ttaagccaaa
     5761 ggtttgtccg tttttttgga caaagtagtt tttttcattt tcatcaataa taatacctgt
     5821 aaatatagtt gctaataaat cgttcatttt tttctccctc tatagtgaaa ggttttctta
     5881 atacatttct ttgctatcat aacataatgt tatctacttg aatagctgag ccttggtttg
     5941 gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac tccccttttt cgaataaacg
     6001 gtgttaaacg gcaggctcac agcctctttc atttgcctaa aattaattgc tcccatttcc
     6061 tccgaaccat ccagttttac agttgatggt tacgatgtaa aatggcaaag cactttctcc
     6121 aatgcctttc atcaatgcag attttgttga agtggcatat agattataat agccttcccc
     6181 attttcgatt ctggcttttt ctaatctgta acctccttca acgatatatc cacgttggat
     6241 acatgtagca atcactttgc tttggacacc tggtgcccac gtccatgctg gattagatgc
     6301 atcggcaata tcacttgctc tggcagattt gattggctga ttcgctgagt ttgaagagga
     6361 aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc
     6421 ttgagctgca gctgctgctt gagcggcttg ttttgcttgt tcttcagctg cttttttcgc
     6481 ttcttctgct tttttctttt cctcttcttc ttttttctct ttatcagtga gcatcttatc
     6541 tacttgtttc aattgttcat tcaactgagc agatagatct gtatttttca ctttttctac
     6601 tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa tccactactt ttccgtcttt
     6661 aaagactata gctgtttttt ctttagctgt ttgcaattca gcatattgat ttttagcttc
     6721 tgtttgtgct tgttgaatca attttccaaa atctgtgtta tccgtttcga ttgcttggat
     6781 ctctttatct gctttcaact tgatgtcatc tgctagatga tcatcgacga tggcaggtgc
     6841 tatgaaccac tcattcactt tttggatatc tcggatctta gattggatat cttggtaagt
     6901 tgtttctaac tctgaatatc ctttttcatt cttaagactg gataaatcat tttttagttt
     6961 tgataaatct tgatgaatca tatctgcacg aataaattga tggtgatcat ctgtataaaa
     7021 ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt ctttttgctt ctgctgctac
     7081 ttcggcttct actttttgat gatggtcata ataagcccag cttcctccac cgagtagaag
     7141 tactgctgct aaaacgagat aaggtacttt ttttgatttt ttcttttgcg ctttatctgg
     7201 atctgttttt tctaagtttg attcttgatg tgttttttca gtatctggca cgattgttat
     7261 tgatttcgct tctgcttcaa acagcttagg ctggttctct tctttttcta tttttgcttc
     7321 aagatcagta ccctctttgc ttacagattg attttcagca tcggattcac tatttatttc
     7381 atcgggctct gttgactttt tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg
     7441 cagtttattc gttttttcct tttctaattg tttttcagta aatacttcag ttttttctgt
     7501 gatatccgtt tttacgtctt ttatttttac tgtttcttta cctgtggcgt tttctctatc
     7561 agagtctttt tcagtttctt gttccttttc tggctctact ttgttttcct gtgcaatttt
     7621 ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt cacgatgtgc
     7681 tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt ctttcagttc
     7741 agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc ctgattcttc
     7801 cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag ttacttgatc
     7861 gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca ttacagattc
     7921 tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg tatcaggaaa
     7981 gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt cttcaattga
     8041 ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac agatagtatt
     8101 ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc gatcggctcc
     8161 tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc caatagctta
     8221 tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa aaaagcttga
     8281 gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt actgatttct
     8341 cttctggtca atagtccacg cttattctaa gaaatctttt aattgttttg aacgagaagg
     8401 atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc gagtaacacc
     8461 aaatactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac caaaacgtaa
     8521 gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt acatcttcca attgttcttt
     8581 tagtaactca taagctgcgt gttctgctgg acttgttgct tcctggtctt ctatgaaatc
     8641 acctaagtga gagtcatctt cttcaccgat tggcgtttca agggaaacag gttcttgcgc
     8701 gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac cgatttcttc
     8761 tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta tcaatttatt
     8821 aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag cgattgctcg
     8881 agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc ctttgcggta
     8941 gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca agtcaaggaa
     9001 ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa ggtttgcttc
     9061 tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc
     9121 ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac gaaccgggtc
     9181 attgattttt acaccggttg gcgcagaaag atcttctgtt tttgcttgtt cagctttttt
     9241 ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca cactgatacc
     9301 tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag tgaatggtgt
     9361 agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt tttctttgat
     9421 gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca taaaagaagg
     9481 ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat gatttcaatc
     9541 gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg catttgtttt
     9601 tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa gtcttggatt
     9661 tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac caaccgcctt
     9721 aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata atgttgtgag
     9781 acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa ctgaaattct
     9841 cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa tagaatctgc
     9901 tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg tctcgtaacc
     9961 tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct tcgaagatcg
    10021 cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag ttgagataga
    10081 tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa ttcttcgagg
    10141 tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata acgtgattta
    10201 aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc ttctccatac
    10261 ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga aagtgctaat
    10321 cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc tgcgttgtct
    10381 ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat ctgttgagct
    10441 gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca tgcagcaatc
    10501 acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc gcttcttgct
    10561 ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg actattcaag
    10621 tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg
    10681 atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa acgatcagac
    10741 atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc atttgaaatt
    10801 gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg
    10861 aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa agctggctgt
    10921 ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc atgagcagca
    10981 atcagtttcc ccgtagcaga ttcaggattt tggacagcta tagtacgctg acgccattga
    11041 tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg aaaacttaga
    11101 ccttctagtt cttgaagaaa agtaaacacg tttccgcctt taccacaacc aaagcaatga
    11161 aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg aaaaggacac
    11221 agccccaagt aatttttacc agactttttc aactggacat attgtccaat cacctcaacg
    11281 atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg agccattttt
    11341 tcaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag gagaataacc
    11401 ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt atacatgttt
    11461 gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca ttaagcatca
    11521 atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca atactcgaag
    11581 aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca accctaagtt
    11641 tacttccaaa attccatgga acagcattgc agctacatat atccctacga tcgtcgttac
    11701 tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg ccatgcaact
    11761 gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc atgcgaatgt
    11821 ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat ttggtaaagc
    11881 attaataagt gctgtaggca aataacggta agtgattttt cttttatctt gttcaaaaga
    11941 taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt ggatagggat
    12001 aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg aataaatcgt
    12061 tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc gtccttcaaa
    12121 caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg caatctgacg
    12181 tgttgctcca tcaccttctg ccatagcaat gctgcaatct gcttctctta acgccaaaac
    12241 atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca attcatttac
    12301 cagcaatttc ttttgctgcg gactcactcg tccaaaaacc gtatagcgat gagcagcttc
    12361 tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat aattcggtaa
    12421 tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa tcactttcag
    12481 atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc ggattggatc
    12541 atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt tcaattgctg
    12601 atttggagcg atccccaaca taagtacgcg atagccattc gcttgggctg tttttagact
    12661 gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca cgactcccag
    12721 ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt tgtttgtcac
    12781 atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga tgttgttatc
    12841 actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt acagcggatg
    12901 tagtgcttct acccgctgga tactcatttt cccttcagta atggtccctg ttttatccaa
    12961 gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa caaggattcg
    13021 tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca ataccaaacc
    13081 ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt tgatagaagc
    13141 atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa tcacgaaact
    13201 agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag ctttgtgagt
    13261 ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga taacttcagc
    13321 catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag tttctttgac
    13381 aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc cagatataac
    13441 ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc ctaataccag
    13501 ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt ctgattggga
    13561 aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa ttccaataac
    13621 gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta caaataacag
    13681 acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat cgctgaatat
    13741 ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag aagccatttt
    13801 ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt tccgccaact
    13861 ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca acttgttttt
    13921 ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac ctttttgttt
    13981 gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt tcctccaaac
    14041 ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa gctagtaccc
    14101 cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa gcaaaaacta
    14161 cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa tccatgattt
    14221 ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc acttcaagtc
    14281 gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc aaaaaattag
    14341 aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca atcagccatg
    14401 ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat aaaaacaaat
    14461 aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt ccttttttcc
    14521 ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa aaccataata
    14581 tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga aagatgcctg
    14641 tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa
    14701 ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct aatgcaccac
    14761 cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca aaccgtaaca
    14821 tccagtcttt ggttgtgtac cccacttctt gattttccat tttttgcctt ttttccacta
    14881 tttgcgtact ccttctttca tttaatttta ttcatgattg aacatttcct gctattttac
    14941 ttaaagactt tttcataggt ctctattatt ttttcattca ttttgacaat atcaggataa
    15001 aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga ctggatccgc
    15061 caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac atctttagaa
    15121 tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata ggcaatataa
    15181 tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg tttctttgct
    15241 tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc tggattatca
    15301 atgatctcag cttctccatg cagactttcc gagctctgca tgagatctgt cccttttcct
    15361 tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat atgataattt
    15421 tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga acgataagtt
    15481 cttcctcata aaaacaacac cgcaccaata accgtcagaa tgatcatgta aaaaagaata
    15541 cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga catctttttt
    15601 tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg tacgccttaa
    15661 gaaaaaatga attttcctgt attttttgac atcttacgaa aatgatcgtt gtttttcagt
    15721 aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg tgattatgtg
    15781 aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta tgaaaagagc
    15841 ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg ctttttttca
    15901 gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag ccgaggacat
    15961 ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga ggatctgaat
    16021 acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag tgatggagca
    16081 aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt gattttaaat
    16141 tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag taatattcat
    16201 tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt
    16261 atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa aatcaactcc
    16321 cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc tttgtatata
    16381 gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca tgaattagct
    16441 agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga aacataacta
    16501 ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga aaaaccacct
    16561 aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg gtgaattgat
    16621 gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca ctatacctgt
    16681 atggaaaagc ggactaatgc tattgattgg tcttatctct gtgttaccta tggttggtta
    16741 tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc tgtttgagac
    16801 aagctggctg atcaatacga tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg
    16861 gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc aagcattatc
    16921 gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt cttttttact
    16981 tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt tgatcggcgg
    17041 cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag gattccttgg
    17101 tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg aatggaccgg
    17161 tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg atctgtggca
    17221 aacaattcca ttattcattg ctgcttcagt gataggaatc gtgtctatga ttccaggaga
    17281 aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg tgccaagaga
    17341 aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc cgtttttgat
    17401 tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt attctggtat
    17461 ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat tatacttttc
    17521 tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt ttcgctggct
    17581 tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta tactattggg
    17641 atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag catatttgcc
    17701 tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt ttagtttcac
    17761 accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa acgaattatt
    17821 tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca ttattggtgc
    17881 actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc ctcatcggcg
    17941 tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt cgggattgtt
    18001 ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc aaggagaaaa
    18061 gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta caacttatgg
    18121 aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg cctatgaaaa
    18181 agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat gtattgtaat
    18241 gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt ttatcgaaga
    18301 aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga ttgttatgat
    18361 tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg tagacctgaa
    18421 tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga atcggatcga
    18481 acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc aaatgagtac
    18541 tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt tttctttagg
    18601 ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa atacccaaga
    18661 agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag taggaacgat
    18721 tgatctgatg cgatatgatt ctaaaaaagc accttctggc agtatggatt tcttgttctt
    18781 gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag ggatggcccc
    18841 tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg catacttggt
    18901 ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata aaagcaagta
    18961 cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga ttgcatatgt
    19021 tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag gtagaataag
    19081 tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata gatgaacact
    19141 ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac aatctcttct
    19201 tatacaattg aagaacaaag agcggtccaa acagaaatcc tgtttggacc gctcttttat
    19261 tttaaacatt tttcattacc aaaatattcg agtcgcgttt cttatgcttc gatctctgta
    19321 acgatacctg aacctactgt ccgtccgcct tcacgaattg agaatgtcgt tccgttttca
    19381 acagcgattg gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg catgaccatt
    19441 tctgtatctt ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa gtagaactgt
    19501 ggacgatagt tgttgaagaa tggagtatga cgtccacctt cttcttttgt caacacgtac
    19561 acttctgctt tgaattttgt atgtggagta atggaacctg gtttagcaag tacttgtcca
    19621 cgttcgatat cgtcacgttg gatcccccgt aacaacacgc ctacgttatc cccagcttcc
    19681 ccgtaatcta acgttttacg gaacatttct acacctgtta caactgcttt ttgtgtttca
    19741 ggtttgatcc ctacgatctc tacctcatca ccgacacgaa cagcaccacg atcaatacga
    19801 cctgaggcaa cagttcctcg acctgtaatt gagaagacgt cttccactgg caatagtaat
    19861 ggtttatctg tatcacgttc tggtgttggg atatattcat ctactgtatc catcaattcc
    19921 ataatagcag cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga acctttgatc
    19981 acaggagtgt catcgcctgg gaaaccatat tcgctcaata actcgcgaac ttccatttct
    20041 accaaatcga tcaattcttc atcatcgact aaatcaactt tgttcaagaa aacaatcagg
    20101 tattttacac caacttggcg tgacaataaa atgtgttcac gtgtttgagg catcggaccg
    20161 tcagttgcag aaacaactaa gattgcacca tccatttgtg ctgcccctgt aatcatgttt
    20221 ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt ttctgtttca
    20281 tattctacgt gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg agctgcatca
    20341 atactagcat agtcttgagg atttgctagg ccttttttgc ctaatactgt agtgatcgca
    20401 gcagttaaag tagttttccc atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt
    20461 ttacttctgt cataatgttc ttttgccata atttacaaac ctcctaaata atatagatca
    20521 ctgaaaaatt tcagctctca cctttttatt atagtctttt tttgactaaa tcaaaaagaa
    20581 tacgtccgac ttactttttg taagattcga actaagctta ttatacgtca atattggtca
    20641 aagtcaacta agagcactcg tctgtcttat acgcgaaaaa agtacctttt ataaataaaa
    20701 aaatgtgttt tttgagaaaa acttcaagat agtccttaac tttcttttga agcaataaga
    20761 acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata gacgaaatct
    20821 tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat tggcgtactt
    20881 gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt ttacggcggc
    20941 gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca agcagataac
    21001 ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga tggtgaaaaa
    21061 aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc gtatcttaaa
    21121 ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc ttctgaagtg
    21181 cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt ttattagata
    21241 aaa
//
LOCUS       DAZKSR010000054        20904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000054 DAZKSR010000000
VERSION     DAZKSR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.054"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG8XG_001759"
     CDS             591..1280
                     /locus_tag="ACG8XG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0773676.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG8XG_001760"
     CDS             1267..2475
                     /locus_tag="ACG8XG_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0773677.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG8XG_001761"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG8XG_001761"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0773678.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8XG_001762"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG8XG_001762"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0773679.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG8XG_001763"
     CDS             complement(6578..7273)
                     /locus_tag="ACG8XG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0773680.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8XG_001764"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG8XG_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0773681.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG8XG_001765"
     CDS             complement(8472..8663)
                     /locus_tag="ACG8XG_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0773682.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG8XG_001766"
     CDS             complement(8684..9574)
                     /locus_tag="ACG8XG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0773683.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG8XG_001767"
     CDS             complement(9561..10328)
                     /locus_tag="ACG8XG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0773684.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8XG_001768"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG8XG_001768"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0773685.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8XG_001769"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG8XG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0773686.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8XG_001770"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG8XG_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0773687.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG8XG_001771"
     CDS             14899..15282
                     /locus_tag="ACG8XG_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773688.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8XG_001772"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG8XG_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0773689.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG8XG_001773"
     CDS             complement(16226..17044)
                     /locus_tag="ACG8XG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0773690.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8XG_001774"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG8XG_001774"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0773691.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8XG_001775"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG8XG_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0773692.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG8XG_001776"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="ACG8XG_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0773693.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG8XG_001777"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="ACG8XG_001777"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0773694.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5976 a         4252 c         3835 g         6841 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgcggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gagtcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa tggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggtgatttc ctgtgtattc cgtctcctaa ctattggtat atctatattt
    17941 tttatccaca gatgcaagag gaaatctatt gatttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgcctcgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atatcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttatg cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatta cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattggctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatcctga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcattg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aagg
//
LOCUS       DAZKSR010000055        20801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000055 DAZKSR010000000
VERSION     DAZKSR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.055"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..300
                     /locus_tag="ACG8XG_001778"
     CDS             <1..300
                     /locus_tag="ACG8XG_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0773695.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(653..1501)
                     /locus_tag="ACG8XG_001779"
                     /pseudo
     CDS             complement(653..1501)
                     /locus_tag="ACG8XG_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1654..2766
                     /locus_tag="ACG8XG_001780"
     CDS             1654..2766
                     /locus_tag="ACG8XG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0773696.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="ACG8XG_001781"
     CDS             complement(2844..3137)
                     /locus_tag="ACG8XG_001781"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0773697.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="ACG8XG_001782"
     CDS             3305..3619
                     /locus_tag="ACG8XG_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0773698.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="ACG8XG_001783"
     CDS             3640..5619
                     /locus_tag="ACG8XG_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0773699.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="ACG8XG_001784"
     CDS             5632..5958
                     /locus_tag="ACG8XG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0773700.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="ACG8XG_001785"
     CDS             5974..6492
                     /locus_tag="ACG8XG_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773701.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="ACG8XG_001786"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="ACG8XG_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0773702.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="ACG8XG_001787"
     CDS             complement(7928..9433)
                     /locus_tag="ACG8XG_001787"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0773703.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="ACG8XG_001788"
     CDS             complement(9525..10115)
                     /locus_tag="ACG8XG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0773704.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="ACG8XG_001789"
     CDS             10294..11940
                     /locus_tag="ACG8XG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0773705.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="ACG8XG_001790"
     CDS             11989..12708
                     /locus_tag="ACG8XG_001790"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0773706.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="ACG8XG_001791"
     CDS             13187..14134
                     /locus_tag="ACG8XG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773707.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="ACG8XG_001792"
     CDS             14146..15066
                     /locus_tag="ACG8XG_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0773708.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="ACG8XG_001793"
     CDS             15107..16564
                     /locus_tag="ACG8XG_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0773709.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="ACG8XG_001794"
     CDS             16909..17523
                     /locus_tag="ACG8XG_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0773710.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="ACG8XG_001795"
     CDS             17516..19240
                     /locus_tag="ACG8XG_001795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0773711.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="ACG8XG_001796"
     CDS             19237..20688
                     /locus_tag="ACG8XG_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0773712.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3510 c         4166 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DAZKSR010000056        20183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000056 DAZKSR010000000
VERSION     DAZKSR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.056"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACG8XG_001797"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACG8XG_001797"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0773713.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACG8XG_001798"
     CDS             1013..2260
                     /locus_tag="ACG8XG_001798"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0773714.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACG8XG_001799"
     CDS             2336..2680
                     /locus_tag="ACG8XG_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773715.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACG8XG_001800"
     CDS             complement(2797..3123)
                     /locus_tag="ACG8XG_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773716.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="ACG8XG_001801"
     CDS             complement(3496..4698)
                     /locus_tag="ACG8XG_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0773717.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="ACG8XG_001802"
     CDS             4815..5330
                     /locus_tag="ACG8XG_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0773718.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="ACG8XG_001803"
     CDS             complement(5529..5975)
                     /locus_tag="ACG8XG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0773719.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACG8XG_001804"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACG8XG_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0773720.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="ACG8XG_001805"
                     /pseudo
     CDS             complement(7854..8483)
                     /locus_tag="ACG8XG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(8467..8931)
                     /locus_tag="ACG8XG_001806"
     CDS             complement(8467..8931)
                     /locus_tag="ACG8XG_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0773721.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="ACG8XG_001807"
     CDS             complement(9418..10686)
                     /locus_tag="ACG8XG_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0773722.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="ACG8XG_001808"
     CDS             complement(10917..11654)
                     /locus_tag="ACG8XG_001808"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0773723.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="ACG8XG_001809"
     CDS             complement(11721..12290)
                     /locus_tag="ACG8XG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0773724.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="ACG8XG_001810"
     CDS             12418..13290
                     /locus_tag="ACG8XG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0773725.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="ACG8XG_001811"
     CDS             13632..14504
                     /locus_tag="ACG8XG_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0773726.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /gene="nagE"
                     /locus_tag="ACG8XG_001812"
     CDS             14501..16459
                     /gene="nagE"
                     /locus_tag="ACG8XG_001812"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0773727.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="ACG8XG_001813"
     CDS             complement(16710..17561)
                     /locus_tag="ACG8XG_001813"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0773728.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="ACG8XG_001814"
     CDS             17684..18151
                     /locus_tag="ACG8XG_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0773729.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="ACG8XG_001815"
     CDS             complement(18414..18896)
                     /locus_tag="ACG8XG_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0773730.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="ACG8XG_001816"
     CDS             complement(18996..19859)
                     /locus_tag="ACG8XG_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0773731.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6069 a         4156 c         3662 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttattcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc ttt
//
LOCUS       DAZKSR010000057        20033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000057 DAZKSR010000000
VERSION     DAZKSR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.057"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1186)
                     /locus_tag="ACG8XG_001817"
     CDS             complement(203..1186)
                     /locus_tag="ACG8XG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0773732.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1191..1838)
                     /locus_tag="ACG8XG_001818"
     CDS             complement(1191..1838)
                     /locus_tag="ACG8XG_001818"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0773733.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1923..2555)
                     /locus_tag="ACG8XG_001819"
     CDS             complement(1923..2555)
                     /locus_tag="ACG8XG_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0773734.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2571..3122)
                     /gene="coaC"
                     /locus_tag="ACG8XG_001820"
     CDS             complement(2571..3122)
                     /gene="coaC"
                     /locus_tag="ACG8XG_001820"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0773735.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3119..3856)
                     /locus_tag="ACG8XG_001821"
     CDS             complement(3119..3856)
                     /locus_tag="ACG8XG_001821"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0773736.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3853..4842)
                     /locus_tag="ACG8XG_001822"
     CDS             complement(3853..4842)
                     /locus_tag="ACG8XG_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0773737.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5100..5642
                     /locus_tag="ACG8XG_001823"
     CDS             5100..5642
                     /locus_tag="ACG8XG_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0773738.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5659..6744
                     /locus_tag="ACG8XG_001824"
     CDS             5659..6744
                     /locus_tag="ACG8XG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773739.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6756..7562
                     /locus_tag="ACG8XG_001825"
     CDS             6756..7562
                     /locus_tag="ACG8XG_001825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773740.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7563..8390
                     /locus_tag="ACG8XG_001826"
     CDS             7563..8390
                     /locus_tag="ACG8XG_001826"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773741.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8387..9460
                     /locus_tag="ACG8XG_001827"
     CDS             8387..9460
                     /locus_tag="ACG8XG_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0773742.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9888..12584
                     /locus_tag="ACG8XG_001828"
     CDS             9888..12584
                     /locus_tag="ACG8XG_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0773743.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12649..13149
                     /locus_tag="ACG8XG_001829"
     CDS             12649..13149
                     /locus_tag="ACG8XG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0773744.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            13161..13655
                     /locus_tag="ACG8XG_001830"
     CDS             13161..13655
                     /locus_tag="ACG8XG_001830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0773745.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13676..14485
                     /locus_tag="ACG8XG_001831"
     CDS             13676..14485
                     /locus_tag="ACG8XG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0773746.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14482..15303
                     /locus_tag="ACG8XG_001832"
     CDS             14482..15303
                     /locus_tag="ACG8XG_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0773747.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15354..17453
                     /locus_tag="ACG8XG_001833"
     CDS             15354..17453
                     /locus_tag="ACG8XG_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0773748.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17450..18568
                     /locus_tag="ACG8XG_001834"
     CDS             17450..18568
                     /locus_tag="ACG8XG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0773749.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18607..19485
                     /locus_tag="ACG8XG_001835"
     CDS             18607..19485
                     /locus_tag="ACG8XG_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0773750.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19631..19828
                     /locus_tag="ACG8XG_001836"
     CDS             19631..19828
                     /locus_tag="ACG8XG_001836"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0773751.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            19812..>20033
                     /locus_tag="ACG8XG_001837"
     CDS             19812..>20033
                     /locus_tag="ACG8XG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773752.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNK"
BASE COUNT         6118 a         3482 c         4282 g         6151 t
ORIGIN      
        1 ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
       61 cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt
      121 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
      181 ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt
      241 tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa
      301 tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg
      361 cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac
      421 gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa
      481 tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc
      541 cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt
      601 gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga
      661 gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt
      721 ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt
      781 gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag
      841 cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag
      901 catattcggc ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag
      961 tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt
     1021 agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg
     1081 tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt
     1141 ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc
     1201 tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc
     1261 gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga
     1321 gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca
     1381 tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat
     1441 tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt
     1501 tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg
     1561 atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg
     1621 gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa
     1681 gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca
     1741 tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg
     1801 ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca
     1861 aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac
     1921 tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca
     1981 gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata
     2041 gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa
     2101 aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag
     2161 aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa
     2221 taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt
     2281 ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg
     2341 ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt
     2401 cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt
     2461 ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac
     2521 atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt
     2581 ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct
     2641 tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca
     2701 gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg
     2761 gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga
     2821 tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga
     2881 aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc
     2941 atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa
     3001 tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt
     3061 gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc
     3121 attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt
     3181 gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga
     3241 tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg
     3301 cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg
     3361 cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca
     3421 caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg
     3481 caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt
     3541 cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat
     3601 cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga
     3661 tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt
     3721 aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc
     3781 ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg
     3841 tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc
     3901 agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag
     3961 ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg
     4021 tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt
     4081 cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat
     4141 caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg
     4201 ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa
     4261 agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg taatgattga
     4321 ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc
     4381 agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa
     4441 catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg
     4501 tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt
     4561 gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt
     4621 actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac
     4681 ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt
     4741 taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa
     4801 aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt
     4861 aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt
     4921 agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa
     4981 gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact
     5041 aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta
     5101 tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac
     5161 taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt
     5221 caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat
     5281 tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt
     5341 atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag
     5401 aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt
     5461 cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga
     5521 cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat
     5581 taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt
     5641 aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc
     5701 tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat
     5761 acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc
     5821 accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct
     5881 aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt
     5941 tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag
     6001 aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt
     6061 gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg
     6121 aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg
     6181 cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat
     6241 gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc
     6301 gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat
     6361 tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt
     6421 gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt
     6481 gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg
     6541 gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa
     6601 aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc
     6661 tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca
     6721 cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt
     6781 ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt
     6841 atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta
     6901 cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt
     6961 ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc
     7021 aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg
     7081 catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga
     7141 tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg
     7201 agattccatt tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga
     7261 tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc
     7321 cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt
     7381 tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg
     7441 aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa
     7501 tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat
     7561 aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg
     7621 caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta
     7681 caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg
     7741 tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat
     7801 ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca
     7861 tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct
     7921 ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca
     7981 gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg
     8041 tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc
     8101 cttatctttc tccaggaatc atagctggat attttatggc atttacctat tctcttgatg
     8161 attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct
     8221 attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct
     8281 tgttctccat gatattggtc attggatact actttattag taaagaaaac cgtccaaaac
     8341 ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca
     8401 atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact
     8461 tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta
     8521 tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga
     8581 aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga
     8641 catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc
     8701 attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga
     8761 ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata
     8821 taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga
     8881 attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa
     8941 tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa
     9001 attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat
     9061 gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg
     9121 ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa
     9181 tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat
     9241 gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga
     9301 agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga
     9361 tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta
     9421 taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat
     9481 ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa
     9541 tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac
     9601 gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag
     9661 taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac
     9721 ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc
     9781 tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa attattttgt
     9841 cagatatctg aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat
     9901 atagaataga catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg
     9961 aaaaaatgaa aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga
    10021 atatgtcttc tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg
    10081 ccagccatta tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc
    10141 ctgttttgtg gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg
    10201 aagcctttca ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta
    10261 tagccgctgt caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg
    10321 ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg
    10381 aaaaagatgc accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc
    10441 tttcggcaac actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg
    10501 atggtttatt ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat
    10561 cgaaagaaaa tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg
    10621 gtgaaaatgt aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag
    10681 gtgaaaatta tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa
    10741 cttttgaaga tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg
    10801 aagcggatgt tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat
    10861 ctcatccctt tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg
    10921 atgtgtatag tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc
    10981 agttaaatat gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga
    11041 aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa
    11101 aaattactca gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg
    11161 aagtacagca tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat
    11221 tgatgattca gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga
    11281 cgacgagtgg acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata
    11341 agacagactt aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact
    11401 tatgtaatca gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa
    11461 gactgcttat tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc
    11521 tgttgcttgc tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat
    11581 gcggcaatta tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg
    11641 tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg
    11701 tagatatggg ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt
    11761 tacttcttct gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga
    11821 tgaatgcgcc atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc
    11881 aatactttga aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg
    11941 ggatttcgga caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag
    12001 ttttcacgaa ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt
    12061 ttgacaagac aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta
    12121 atattaatgt ttatgacatc ttagacggtg atggagcagc caatttggag catattttga
    12181 gtcctgaaat gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt
    12241 cgttggaagg aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag
    12301 tggaaacagt catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa
    12361 aactaaatct ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag
    12421 aaaaatctga agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt
    12481 ctagaagtat ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc
    12541 tttcgctgct ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg
    12601 tctaatctgt tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca
    12661 atgtttttaa aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt
    12721 aaccatatga agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc
    12781 agtattcaat tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat
    12841 gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag
    12901 atttttgttt ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt
    12961 ttggagaaag agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa
    13021 atagtattgg caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc
    13081 caacaggaat taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat
    13141 tttttttagg aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg
    13201 gacaagtagc gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg
    13261 atgaggcggc taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg
    13321 tcaaagtcat tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga
    13381 aggacatgcg cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag
    13441 ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat
    13501 cggaagataa gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact
    13561 taaaagaatt agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa
    13621 aacgttcagc tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta
    13681 tgatgcattt gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg
    13741 gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga
    13801 tttgcagacg gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa
    13861 tatcggaatc tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc
    13921 aatcaaaatg ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt
    13981 aggattacag ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt
    14041 tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg
    14101 gtttgtccac tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga
    14161 tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc
    14221 tggaaatctg ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa
    14281 tattgctgtg tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat
    14341 tgcaattgcc gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact
    14401 aaaaaatata tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca
    14461 agaagaggag gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa
    14521 ttggttcgga agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg
    14581 atgggcggcg gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt
    14641 gaagaagatc gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct
    14701 tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct
    14761 ggttttgacg tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga
    14821 attggcgatt cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac
    14881 ttagccaatg ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct
    14941 aattacttga tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt
    15001 gaaaagctgt atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt
    15061 ctgatcatgg taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt
    15121 tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga
    15181 attgtaccgt tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc
    15241 atcaaaatta tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca
    15301 tagtaggaag ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta
    15361 ttaatgaagt aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa
    15421 atgtgactac ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga
    15481 ccttcgatgt aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa
    15541 cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata
    15601 aacagctctc tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc
    15661 atttgtcaga atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat
    15721 atattcatca ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta
    15781 ataagcgttt aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc
    15841 ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg
    15901 tgttgatttc tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg
    15961 ccattgcttt tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata
    16021 tcgaaaaatg gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa
    16081 tgccaaacga tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt
    16141 tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta
    16201 atcgaatttg gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg
    16261 agttatgtca aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa
    16321 aatttacagg aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta
    16381 gtctttattt tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac
    16441 tgaatgcttc aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc
    16501 tttttccatc tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca
    16561 atctttgtct ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac
    16621 tgagtcttag caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac
    16681 aaatgactat cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga
    16741 aaacaaccta ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt
    16801 taagtgatgt gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag
    16861 acactacgtt gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca
    16921 ccatggcctt ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca
    16981 cttggggatt agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc
    17041 tgcacaaccc tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag
    17101 acggttcgtg gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag
    17161 actttggctg cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc
    17221 aatatttcgg attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa
    17281 cgttttcttg gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat
    17341 ccgagttgag tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa
    17401 ttcttcacgt agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact
    17461 aaaaaatatt acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac
    17521 aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg
    17581 tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg
    17641 aataattcgt catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt
    17701 acgtgattta atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc
    17761 tttaggggat ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat
    17821 agtggttaat cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc
    17881 taaaattacg atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa
    17941 ttacgctttg gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg
    18001 ggaacgcatt accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg
    18061 ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg
    18121 cgagtttaaa ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc
    18181 agaagattat tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca
    18241 tgcgaaaaac tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac
    18301 aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat
    18361 gccacgacat ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt
    18421 atcggtcact ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga
    18481 tgttgctagt gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt
    18541 cactgatcga gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa
    18601 cctatgatga tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt
    18661 actgaggaat taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca
    18721 atgaagcagg tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc
    18781 ggctgctttg gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca
    18841 acaccaaaac ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat
    18901 attccgattg aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt
    18961 tctggcaaag ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt
    19021 gccttgttca atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg
    19081 atcaagcgac atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta
    19141 gaggggatgg cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta
    19201 ttggctgacg attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat
    19261 acaacactga tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa
    19321 cacatgaaga aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata
    19381 ccaccaattg atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc
    19441 ggattagcat tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca
    19501 ttgatactca tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa
    19561 agcgaaaatg aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca
    19621 ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag
    19681 gatattcact ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt
    19741 aatagttata gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct
    19801 tggcttaatg tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta
    19861 attctattat agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta
    19921 tacattttaa atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac
    19981 ctttaactct cgcttctctt gcaaatgtag ttagcatttt aatcgataat aaa
//
LOCUS       DAZKSR010000058        20003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000058 DAZKSR010000000
VERSION     DAZKSR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.058"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..604)
                     /locus_tag="ACG8XG_001838"
     CDS             complement(74..604)
                     /locus_tag="ACG8XG_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0773753.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(786..1442)
                     /locus_tag="ACG8XG_001839"
     CDS             complement(786..1442)
                     /locus_tag="ACG8XG_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFQ0773754.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1547..2042)
                     /gene="dfrF"
                     /locus_tag="ACG8XG_001840"
                     /pseudo
     CDS             complement(1547..2042)
                     /gene="dfrF"
                     /locus_tag="ACG8XG_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
     gene            complement(2437..3828)
                     /gene="amt"
                     /locus_tag="ACG8XG_001841"
     CDS             complement(2437..3828)
                     /gene="amt"
                     /locus_tag="ACG8XG_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0773755.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(3914..4141)
                     /locus_tag="ACG8XG_001842"
     CDS             complement(3914..4141)
                     /locus_tag="ACG8XG_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773756.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(4169..4411)
                     /locus_tag="ACG8XG_001843"
     CDS             complement(4169..4411)
                     /locus_tag="ACG8XG_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0773757.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4443..5000)
                     /locus_tag="ACG8XG_001844"
     CDS             complement(4443..5000)
                     /locus_tag="ACG8XG_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0773758.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5013..5201)
                     /locus_tag="ACG8XG_001845"
     CDS             complement(5013..5201)
                     /locus_tag="ACG8XG_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0773759.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="ACG8XG_001846"
     CDS             complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="ACG8XG_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0773760.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6197..6370
                     /locus_tag="ACG8XG_001847"
     CDS             6197..6370
                     /locus_tag="ACG8XG_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773761.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7010..7483
                     /locus_tag="ACG8XG_001848"
     CDS             7010..7483
                     /locus_tag="ACG8XG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0773762.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7492..7719
                     /locus_tag="ACG8XG_001849"
     CDS             7492..7719
                     /locus_tag="ACG8XG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773763.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            8355..8726
                     /locus_tag="ACG8XG_001850"
     CDS             8355..8726
                     /locus_tag="ACG8XG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773764.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(8982..9224)
                     /locus_tag="ACG8XG_001851"
     CDS             complement(8982..9224)
                     /locus_tag="ACG8XG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0773765.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(9256..10158)
                     /locus_tag="ACG8XG_001852"
     CDS             complement(9256..10158)
                     /locus_tag="ACG8XG_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0773766.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(10171..10359)
                     /locus_tag="ACG8XG_001853"
     CDS             complement(10171..10359)
                     /locus_tag="ACG8XG_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0773767.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(10373..10936)
                     /gene="amaP"
                     /locus_tag="ACG8XG_001854"
     CDS             complement(10373..10936)
                     /gene="amaP"
                     /locus_tag="ACG8XG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0773768.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(10974..11867)
                     /locus_tag="ACG8XG_001855"
     CDS             complement(10974..11867)
                     /locus_tag="ACG8XG_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0773769.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(11945..12868)
                     /locus_tag="ACG8XG_001856"
     CDS             complement(11945..12868)
                     /locus_tag="ACG8XG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0773770.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(12917..13267)
                     /locus_tag="ACG8XG_001857"
     CDS             complement(12917..13267)
                     /locus_tag="ACG8XG_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0773771.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(13300..14202)
                     /locus_tag="ACG8XG_001858"
     CDS             complement(13300..14202)
                     /locus_tag="ACG8XG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0773772.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(14195..15052)
                     /locus_tag="ACG8XG_001859"
     CDS             complement(14195..15052)
                     /locus_tag="ACG8XG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0773773.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            15386..16195
                     /locus_tag="ACG8XG_001860"
     CDS             15386..16195
                     /locus_tag="ACG8XG_001860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0773774.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            16235..16732
                     /locus_tag="ACG8XG_001861"
     CDS             16235..16732
                     /locus_tag="ACG8XG_001861"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0773775.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(17117..17190)
                     /locus_tag="ACG8XG_001862"
     tRNA            complement(17117..17190)
                     /locus_tag="ACG8XG_001862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17154..17156),aa:Ile,seq:cat)
     gene            17377..17700
                     /locus_tag="ACG8XG_001863"
     CDS             17377..17700
                     /locus_tag="ACG8XG_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773776.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            17864..18118
                     /locus_tag="ACG8XG_001864"
     CDS             17864..18118
                     /locus_tag="ACG8XG_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773777.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            18188..18433
                     /locus_tag="ACG8XG_001865"
     CDS             18188..18433
                     /locus_tag="ACG8XG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773778.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            18509..18874
                     /locus_tag="ACG8XG_001866"
     CDS             18509..18874
                     /locus_tag="ACG8XG_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0773779.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(18935..19498)
                     /locus_tag="ACG8XG_001867"
     CDS             complement(18935..19498)
                     /locus_tag="ACG8XG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773780.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         5912 a         3851 c         3193 g         7047 t
ORIGIN      
        1 aaatcttaag gattttcccg ccaacgtggt cggatcgctc cgtcggttta acagcttttc
       61 tgtttctctc tagttatcta cgcatataaa tcaaatcggt tattccgtta taagtctgtg
      121 ttttacaaag ccttaacttt atttccctgt ctatgttgcc caaaagacga accccatttc
      181 ctaacagagt aggaataaca gaaatgtaat atttatcaat catatttttg cttaccaact
      241 gctgaacaag atttgctccg ccacaaatcc aaatatcctt tcctgcttct tgttttagcc
      301 gttccaataa taaaacagga ttttcacttg taaaatgaat ttgttgggaa gaattgcact
      361 ctttatgtgt taatacataa gttgtaaatt tattatatac ccattcttgc ggagaaagtt
      421 cagtaacaac ctgatgagaa gtattccacc ccattaagac agtatctata tctttggtaa
      481 attctgtata agtgtcgatg ttttcattgt catttccatg accatttagc caagctactc
      541 caccattacc gtccgcaata tatccgtcaa ggctcatggc aataaataaa actacttttc
      601 tcattttttc tcttttataa atacaaacag gaatttattt ctctttttta ctcataaggg
      661 aacaactgat tgctacaaat aaacccatta aaatccatgg aaatgctgct cttataaaat
      721 cactaaccat actttattcc tccttaaatt ccaatttcct tagttctaca aactgaaatt
      781 tttgcctatg aatgtatctt gttctttata cgcaaacagg cttctgtgtg cataatccaa
      841 tgtcttgaaa acggcatttg aattagtcca caaatatctt tattacacca ataatcaatc
      901 agccaaattg ctttttcatt gtcgtttact acattcaacg attctaagta tccttttctt
      961 tctttcggta tttttctttt caattcatca taagataacc gttcaagcat tttctcagtg
     1021 ctttcccata cttcgaaaat atataaatat aattcttcca gattaagctg ttttgaaaag
     1081 tctgcaatct gctgtttcat gagttcattt cctgttgtaa tgataggtga attgatacgc
     1141 tcttgataat tgcctgcaaa aaatacttgt tcatcttcat ttatcactgt atgcacaact
     1201 atatcttcaa tgcggaaaat atgccaaata gaataagcaa tcgtcttact atgataacca
     1261 tcagcgttta tgaaggggat tgagttaaaa tcttctctgc ataattcttc tttgaaagat
     1321 actaaggttt gtattaactg acttcgtaaa gtaagtaaag tatcaatacc cctcttataa
     1381 gtatctttct tttttatttg tgcctgcatt gttttattaa gttcagacca ttccttattc
     1441 atattcaatc ctctctcaaa ttctgatttt atcgccccat acaaactgga atttgtctta
     1501 caacaattta tatatgcata aaaagcaata tatttatgaa aacaccctaa atccaaaatt
     1561 tactcaattc attttttcct tacataaaat gttctcgtat atttcacttc ctcaccaagt
     1621 gtttccccta tcaaaacttc aaaatcattg atatcaaatt ctggaaagaa tgtatctcca
     1681 tcttcaatat ttaaatctac ttctgtgata tacattttat ctactatttg caaagcttcc
     1741 ttaaatagtc catatccacc agatatgtat acatctcgtc ctttagccaa taataatgca
     1801 tcttctaatg atttaactga aactaaatta tctccttgat actctgttgt ggtggaaaca
     1861 acaatattca ttctattagg caacggatga ccgatttctt cataagactt tcgccccata
     1921 ataaccacat tacccgttgt taactctcta aattgctttt gttctccctt tattttccat
     1981 ggtatattac cattcttgcc tataacatta ttctttgacc tcgcaacaat caaacctatc
     2041 atctatttca tcctccataa tatgatgtcg tacctgtgat tccatcaagt gcaccacatc
     2101 ttaacaaact caaaattatc tcgaactaac cagctcgaca aattcctttt ttgtcttcat
     2161 ctgcattata tcatattgtt ataagcattg ccatttcttt tctgcttttg aaaagttact
     2221 tatcagccta tgttttgtaa agggtgatat tcagcatttc tcacgcaaca agcccttgac
     2281 aaaccataga ctaataagtt ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca
     2341 cggcaacaaa aaagagatac caacacactt atgtagtatc tcttagtcta gtagcaatat
     2401 tcttttgttt gcaagcaggt aaacatgggc tgtttcttaa tatctttttg tttgggaaac
     2461 acctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
     2521 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
     2581 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaagaa
     2641 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
     2701 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
     2761 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
     2821 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
     2881 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
     2941 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
     3001 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
     3061 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
     3121 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
     3181 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
     3241 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
     3301 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
     3361 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
     3421 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
     3481 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
     3541 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
     3601 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
     3661 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
     3721 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
     3781 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
     3841 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
     3901 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
     3961 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
     4021 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat
     4081 ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca
     4141 tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta
     4201 atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc
     4261 cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg
     4321 cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac
     4381 cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact
     4441 tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca
     4501 gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt
     4561 actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca
     4621 acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc
     4681 aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct
     4741 agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct
     4801 gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca
     4861 tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat
     4921 tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta
     4981 ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt
     5041 cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac
     5101 aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca
     5161 aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc
     5221 actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact
     5281 gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc
     5341 atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg
     5401 atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct
     5461 tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca
     5521 tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag
     5581 atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag
     5641 taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca
     5701 ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt
     5761 atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac
     5821 tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc
     5881 taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat
     5941 tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat attattcaag
     6001 attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta tttattaaat
     6061 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
     6121 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
     6181 aaatgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
     6241 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
     6301 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
     6361 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
     6421 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
     6481 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
     6541 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
     6601 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
     6661 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
     6721 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
     6781 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
     6841 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
     6901 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
     6961 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
     7021 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     7081 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     7141 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     7201 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     7261 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     7321 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     7381 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     7441 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     7501 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     7561 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     7621 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     7681 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     7741 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     7801 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     7861 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     7921 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     7981 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     8041 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     8101 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     8161 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     8221 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     8281 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     8341 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     8401 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     8461 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     8521 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     8581 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     8641 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     8701 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     8761 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     8821 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     8881 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     8941 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     9001 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     9061 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     9121 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     9181 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     9241 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     9301 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     9361 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     9421 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     9481 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     9541 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     9601 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     9661 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     9721 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     9781 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     9841 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     9901 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     9961 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
    10021 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
    10081 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
    10141 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
    10201 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
    10261 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
    10321 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
    10381 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
    10441 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
    10501 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
    10561 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
    10621 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
    10681 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
    10741 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
    10801 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
    10861 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
    10921 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
    10981 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
    11041 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
    11101 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
    11161 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
    11221 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
    11281 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
    11341 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
    11401 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
    11461 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
    11521 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
    11581 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
    11641 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
    11701 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
    11761 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
    11821 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
    11881 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
    11941 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
    12001 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
    12061 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
    12121 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
    12181 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
    12241 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
    12301 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
    12361 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
    12421 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
    12481 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
    12541 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
    12601 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
    12661 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
    12721 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
    12781 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
    12841 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
    12901 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
    12961 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
    13021 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
    13081 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
    13141 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
    13201 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
    13261 tttccattcc tactccccct cttcagttgt ggtcttctat tatttaacaa ctacatctgt
    13321 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
    13381 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
    13441 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
    13501 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
    13561 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
    13621 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
    13681 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
    13741 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
    13801 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
    13861 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
    13921 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
    13981 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
    14041 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
    14101 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
    14161 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
    14221 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
    14281 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
    14341 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
    14401 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
    14461 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
    14521 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
    14581 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
    14641 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
    14701 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
    14761 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
    14821 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
    14881 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
    14941 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
    15001 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
    15061 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
    15121 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
    15181 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
    15241 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
    15301 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
    15361 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
    15421 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
    15481 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
    15541 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
    15601 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
    15661 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
    15721 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
    15781 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
    15841 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
    15901 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
    15961 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
    16021 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    16081 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    16141 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    16201 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    16261 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    16321 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    16381 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    16441 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    16501 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    16561 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    16621 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    16681 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    16741 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    16801 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    16861 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    16921 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    16981 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    17041 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    17101 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    17161 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    17221 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    17281 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    17341 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    17401 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    17461 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    17521 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    17581 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    17641 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    17701 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    17761 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    17821 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    17881 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    17941 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    18001 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    18061 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    18121 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    18181 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    18241 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    18301 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    18361 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    18421 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    18481 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    18541 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    18601 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    18661 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    18721 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    18781 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    18841 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    18901 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    18961 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    19021 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    19081 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    19141 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    19201 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    19261 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    19321 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    19381 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    19441 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    19501 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    19561 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    19621 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    19681 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    19741 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    19801 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    19861 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    19921 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    19981 attcagccca ttcaacgacc aat
//
LOCUS       DAZKSR010000059        19804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000059 DAZKSR010000000
VERSION     DAZKSR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.059"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1309
                     /locus_tag="ACG8XG_001868"
     CDS             50..1309
                     /locus_tag="ACG8XG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0773781.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1398..2345)
                     /locus_tag="ACG8XG_001869"
     CDS             complement(1398..2345)
                     /locus_tag="ACG8XG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0773782.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2739..4031
                     /gene="hisS"
                     /locus_tag="ACG8XG_001870"
     CDS             2739..4031
                     /gene="hisS"
                     /locus_tag="ACG8XG_001870"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0773783.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4046..5815
                     /gene="aspS"
                     /locus_tag="ACG8XG_001871"
     CDS             4046..5815
                     /gene="aspS"
                     /locus_tag="ACG8XG_001871"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0773784.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5924..6799
                     /locus_tag="ACG8XG_001872"
     CDS             5924..6799
                     /locus_tag="ACG8XG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0773785.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7065..8948
                     /locus_tag="ACG8XG_001873"
     CDS             7065..8948
                     /locus_tag="ACG8XG_001873"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0773786.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9023..9307)
                     /locus_tag="ACG8XG_001874"
     CDS             complement(9023..9307)
                     /locus_tag="ACG8XG_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0773787.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9297..10031)
                     /locus_tag="ACG8XG_001875"
     CDS             complement(9297..10031)
                     /locus_tag="ACG8XG_001875"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0773788.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10160..10783
                     /locus_tag="ACG8XG_001876"
     CDS             10160..10783
                     /locus_tag="ACG8XG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0773789.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            10975..12096
                     /locus_tag="ACG8XG_001877"
     CDS             10975..12096
                     /locus_tag="ACG8XG_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0773790.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12103..15231
                     /locus_tag="ACG8XG_001878"
     CDS             12103..15231
                     /locus_tag="ACG8XG_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0773791.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15250..18444
                     /locus_tag="ACG8XG_001879"
     CDS             15250..18444
                     /locus_tag="ACG8XG_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0773792.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18590..19297)
                     /locus_tag="ACG8XG_001880"
     CDS             complement(18590..19297)
                     /locus_tag="ACG8XG_001880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0773793.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6635 a         3480 c         4165 g         5524 t
ORIGIN      
        1 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
       61 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
      121 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
      181 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
      241 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
      301 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
      361 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
      421 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
      481 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
      541 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
      601 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
      661 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
      721 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
      781 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
      841 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
      901 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
      961 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
     1021 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
     1081 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
     1141 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
     1201 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
     1261 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
     1321 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
     1381 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
     1441 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
     1501 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
     1561 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
     1621 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
     1681 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
     1741 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
     1801 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
     1861 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
     1921 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
     1981 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
     2041 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
     2101 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
     2161 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
     2221 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
     2281 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
     2341 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
     2401 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
     2461 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
     2521 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
     2581 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
     2641 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
     2701 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
     2761 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
     2821 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
     2881 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
     2941 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
     3001 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
     3061 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
     3121 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
     3181 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
     3241 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
     3301 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
     3361 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
     3421 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
     3481 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
     3541 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
     3601 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
     3661 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
     3721 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
     3781 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
     3841 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
     3901 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
     3961 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
     4021 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
     4081 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
     4141 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
     4201 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
     4261 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
     4321 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
     4381 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
     4441 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
     4501 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
     4561 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
     4621 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
     4681 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
     4741 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
     4801 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
     4861 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
     4921 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
     4981 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
     5041 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
     5101 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
     5161 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
     5221 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
     5281 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
     5341 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
     5401 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
     5461 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
     5521 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
     5581 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
     5641 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
     5701 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
     5761 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
     5821 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
     5881 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
     5941 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
     6001 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
     6061 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
     6121 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
     6181 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
     6241 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
     6301 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
     6361 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
     6421 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
     6481 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
     6541 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
     6601 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
     6661 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
     6721 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
     6781 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
     6841 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
     6901 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
     6961 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
     7021 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
     7081 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
     7141 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
     7201 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
     7261 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
     7321 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
     7381 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
     7441 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
     7501 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
     7561 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
     7621 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
     7681 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
     7741 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
     7801 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
     7861 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
     7921 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
     7981 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
     8041 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
     8101 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
     8161 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
     8221 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
     8281 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
     8341 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
     8401 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
     8461 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
     8521 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
     8581 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
     8641 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
     8701 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
     8761 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
     8821 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
     8881 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
     8941 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
     9001 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
     9061 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
     9121 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
     9181 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
     9241 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
     9301 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
     9361 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
     9421 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
     9481 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
     9541 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
     9601 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
     9661 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
     9721 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
     9781 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
     9841 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
     9901 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
     9961 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    10021 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    10081 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    10141 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    10201 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    10261 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    10321 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    10381 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    10441 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    10501 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    10561 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    10621 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    10681 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    10741 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    10801 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    10861 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    10921 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    10981 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    11041 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    11101 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    11161 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    11221 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    11281 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    11341 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    11401 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    11461 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    11521 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    11581 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    11641 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    11701 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    11761 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    11821 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    11881 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    11941 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    12001 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    12061 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    12121 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    12181 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    12241 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    12301 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    12361 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    12421 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    12481 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    12541 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    12601 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    12661 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    12721 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    12781 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    12841 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    12901 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    12961 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    13021 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    13081 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    13141 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    13201 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    13261 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    13321 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    13381 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    13441 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    13501 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    13561 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    13621 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    13681 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
    13741 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    13801 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    13861 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    13921 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    13981 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    14041 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    14101 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    14161 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    14221 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    14281 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    14341 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    14401 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    14461 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    14521 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    14581 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    14641 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    14701 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    14761 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    14821 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    14881 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    14941 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    15001 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    15061 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    15121 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    15181 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    15241 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    15301 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    15361 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    15421 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    15481 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    15541 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    15601 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    15661 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    15721 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    15781 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    15841 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    15901 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    15961 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    16021 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    16081 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    16141 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    16201 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    16261 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    16321 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    16381 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    16441 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    16501 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    16561 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    16621 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    16681 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    16741 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    16801 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    16861 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    16921 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    16981 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    17041 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    17101 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    17161 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    17221 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    17281 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    17341 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    17401 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    17461 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    17521 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    17581 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    17641 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    17701 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    17761 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    17821 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    17881 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    17941 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    18001 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    18061 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    18121 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    18181 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    18241 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    18301 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    18361 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    18421 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    18481 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    18541 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    18601 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    18661 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    18721 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    18781 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    18841 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    18901 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    18961 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    19021 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    19081 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    19141 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    19201 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    19261 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    19321 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    19381 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    19441 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    19501 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    19561 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    19621 tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacaga
    19681 gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac attactatta
    19741 aagaaataaa aaacataaaa aatcttaaga aatcggtttc atttagcttt tgagataaaa
    19801 taat
//
LOCUS       DAZKSR010000060        19455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000060 DAZKSR010000000
VERSION     DAZKSR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.060"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG8XG_001881"
     CDS             131..3658
                     /locus_tag="ACG8XG_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0773794.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG8XG_001882"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG8XG_001882"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0773795.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG8XG_001883"
     CDS             7446..7787
                     /locus_tag="ACG8XG_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0773796.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8XG_001884"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8XG_001884"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0773797.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8XG_001885"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8XG_001885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0773798.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8XG_001886"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8XG_001886"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0773799.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACG8XG_001887"
     CDS             12998..13735
                     /locus_tag="ACG8XG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773800.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACG8XG_001888"
     CDS             13749..14564
                     /locus_tag="ACG8XG_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0773801.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACG8XG_001889"
     CDS             14577..15224
                     /locus_tag="ACG8XG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0773802.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACG8XG_001890"
     CDS             15240..15896
                     /locus_tag="ACG8XG_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0773803.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACG8XG_001891"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACG8XG_001891"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0773804.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACG8XG_001892"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACG8XG_001892"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0773805.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACG8XG_001893"
     CDS             18238..18756
                     /locus_tag="ACG8XG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0773806.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8XG_001894"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8XG_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0773807.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagc
//
LOCUS       DAZKSR010000061        18805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000061 DAZKSR010000000
VERSION     DAZKSR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.061"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8XG_001895"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8XG_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0773808.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="ACG8XG_001896"
     CDS             complement(1692..2711)
                     /locus_tag="ACG8XG_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0773809.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="ACG8XG_001897"
     CDS             complement(2733..3740)
                     /locus_tag="ACG8XG_001897"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0773810.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8XG_001898"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8XG_001898"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0773811.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="ACG8XG_001899"
     CDS             complement(4391..6685)
                     /locus_tag="ACG8XG_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0773812.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="ACG8XG_001900"
     CDS             6945..9110
                     /locus_tag="ACG8XG_001900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0773813.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="ACG8XG_001901"
     CDS             9414..10247
                     /locus_tag="ACG8XG_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0773814.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="ACG8XG_001902"
     CDS             complement(10349..12049)
                     /locus_tag="ACG8XG_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773815.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="ACG8XG_001903"
     CDS             12556..13716
                     /locus_tag="ACG8XG_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0773816.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="ACG8XG_001904"
     CDS             13732..14175
                     /locus_tag="ACG8XG_001904"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0773817.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="ACG8XG_001905"
     CDS             complement(14658..15188)
                     /locus_tag="ACG8XG_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0773818.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="ACG8XG_001906"
     CDS             15293..15844
                     /locus_tag="ACG8XG_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0773819.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="ACG8XG_001907"
     CDS             complement(15997..16470)
                     /locus_tag="ACG8XG_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0773820.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="ACG8XG_001908"
     CDS             16608..18080
                     /locus_tag="ACG8XG_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0773821.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="ACG8XG_001909"
     CDS             18077..18787
                     /locus_tag="ACG8XG_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0773822.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5760 a         3399 c         3884 g         5762 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgta
//
LOCUS       DAZKSR010000062        18655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000062 DAZKSR010000000
VERSION     DAZKSR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.062"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..278
                     /locus_tag="ACG8XG_001910"
                     /pseudo
     CDS             150..278
                     /locus_tag="ACG8XG_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="ACG8XG_001911"
     CDS             392..631
                     /locus_tag="ACG8XG_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773823.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="ACG8XG_001912"
     CDS             743..937
                     /locus_tag="ACG8XG_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773824.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="ACG8XG_001913"
     CDS             1023..1694
                     /locus_tag="ACG8XG_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0773825.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="ACG8XG_001914"
     CDS             1747..3723
                     /locus_tag="ACG8XG_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0773826.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="ACG8XG_001915"
     CDS             3738..4490
                     /locus_tag="ACG8XG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0773827.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="ACG8XG_001916"
     CDS             4506..5261
                     /locus_tag="ACG8XG_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0773828.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="ACG8XG_001917"
     CDS             5274..5534
                     /locus_tag="ACG8XG_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773829.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="ACG8XG_001918"
     CDS             5531..7621
                     /locus_tag="ACG8XG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0773830.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="ACG8XG_001919"
     CDS             7648..8112
                     /locus_tag="ACG8XG_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773831.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="ACG8XG_001920"
     CDS             8109..8351
                     /locus_tag="ACG8XG_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0773832.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="ACG8XG_001921"
     CDS             8369..8671
                     /locus_tag="ACG8XG_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773833.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="ACG8XG_001922"
     CDS             8741..10804
                     /locus_tag="ACG8XG_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773834.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="ACG8XG_001923"
     CDS             10804..13416
                     /locus_tag="ACG8XG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0773835.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15944
                     /locus_tag="ACG8XG_001924"
     CDS             13413..15944
                     /locus_tag="ACG8XG_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0773836.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15994..16326
                     /locus_tag="ACG8XG_001925"
     CDS             15994..16326
                     /locus_tag="ACG8XG_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773837.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="ACG8XG_001926"
     CDS             16327..16920
                     /locus_tag="ACG8XG_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773838.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..>18655
                     /locus_tag="ACG8XG_001927"
     CDS             17083..>18655
                     /locus_tag="ACG8XG_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0773839.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6891 a         2925 c         3836 g         5003 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgac
//
LOCUS       DAZKSR010000063        18022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000063 DAZKSR010000000
VERSION     DAZKSR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.063"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACG8XG_001928"
     CDS             214..498
                     /locus_tag="ACG8XG_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0773840.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACG8XG_001929"
     CDS             complement(815..1198)
                     /locus_tag="ACG8XG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0773841.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACG8XG_001930"
     CDS             complement(1200..1784)
                     /locus_tag="ACG8XG_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0773842.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACG8XG_001931"
     CDS             complement(1831..3306)
                     /locus_tag="ACG8XG_001931"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0773843.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACG8XG_001932"
     CDS             3487..3798
                     /locus_tag="ACG8XG_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773844.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACG8XG_001933"
     CDS             complement(3913..6249)
                     /locus_tag="ACG8XG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0773845.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACG8XG_001934"
     CDS             6391..7059
                     /locus_tag="ACG8XG_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0773846.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACG8XG_001935"
     CDS             7062..8105
                     /locus_tag="ACG8XG_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0773847.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACG8XG_001936"
     CDS             complement(8342..9223)
                     /locus_tag="ACG8XG_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0773848.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACG8XG_001937"
     CDS             9382..10110
                     /locus_tag="ACG8XG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0773849.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACG8XG_001938"
     CDS             10266..10583
                     /locus_tag="ACG8XG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0773850.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACG8XG_001939"
     CDS             10576..11184
                     /locus_tag="ACG8XG_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0773851.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACG8XG_001940"
     CDS             11239..12030
                     /locus_tag="ACG8XG_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0773852.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACG8XG_001941"
     CDS             12168..12656
                     /locus_tag="ACG8XG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0773853.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACG8XG_001942"
     CDS             12707..13579
                     /locus_tag="ACG8XG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0773854.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACG8XG_001943"
     CDS             13755..14117
                     /locus_tag="ACG8XG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0773855.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACG8XG_001944"
     CDS             14126..14878
                     /locus_tag="ACG8XG_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773856.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACG8XG_001945"
     CDS             14892..16694
                     /locus_tag="ACG8XG_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773857.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACG8XG_001946"
     CDS             16971..18011
                     /locus_tag="ACG8XG_001946"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0773858.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5657 a         3126 c         3723 g         5516 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aa
//
LOCUS       DAZKSR010000064        17418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000064 DAZKSR010000000
VERSION     DAZKSR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.064"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..551
                     /locus_tag="ACG8XG_001947"
     CDS             48..551
                     /locus_tag="ACG8XG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0773859.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            678..1715
                     /locus_tag="ACG8XG_001948"
     CDS             678..1715
                     /locus_tag="ACG8XG_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFQ0773860.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1730..3427
                     /locus_tag="ACG8XG_001949"
     CDS             1730..3427
                     /locus_tag="ACG8XG_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFQ0773861.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3420..3881
                     /locus_tag="ACG8XG_001950"
     CDS             3420..3881
                     /locus_tag="ACG8XG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFQ0773862.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3894..4754
                     /locus_tag="ACG8XG_001951"
     CDS             3894..4754
                     /locus_tag="ACG8XG_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773863.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4792..5181
                     /locus_tag="ACG8XG_001952"
     CDS             4792..5181
                     /locus_tag="ACG8XG_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0773864.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5168..7615
                     /locus_tag="ACG8XG_001953"
     CDS             5168..7615
                     /locus_tag="ACG8XG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0773865.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7620..9728
                     /locus_tag="ACG8XG_001954"
     CDS             7620..9728
                     /locus_tag="ACG8XG_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0773866.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9725..10729
                     /locus_tag="ACG8XG_001955"
     CDS             9725..10729
                     /locus_tag="ACG8XG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0773867.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10748..11659
                     /locus_tag="ACG8XG_001956"
     CDS             10748..11659
                     /locus_tag="ACG8XG_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0773868.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11846..12133
                     /locus_tag="ACG8XG_001957"
                     /pseudo
     CDS             11846..12133
                     /locus_tag="ACG8XG_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12321..12536)
                     /locus_tag="ACG8XG_001958"
     CDS             complement(12321..12536)
                     /locus_tag="ACG8XG_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773869.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12795..13004)
                     /locus_tag="ACG8XG_001959"
                     /pseudo
     CDS             complement(12795..13004)
                     /locus_tag="ACG8XG_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13307..14746)
                     /locus_tag="ACG8XG_001960"
                     /pseudo
     CDS             complement(13307..14746)
                     /locus_tag="ACG8XG_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15294..15965)
                     /locus_tag="ACG8XG_001961"
     CDS             complement(15294..15965)
                     /locus_tag="ACG8XG_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0773870.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16314..>17418)
                     /locus_tag="ACG8XG_001962"
     CDS             complement(16314..>17418)
                     /locus_tag="ACG8XG_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0773871.1"
                     /translation="SSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         5714 a         2931 c         3370 g         5403 t
ORIGIN      
        1 tactcaattt gtttaaccga attgtcggtg cctgaggggg aatcacaatg gaacagatgc
       61 gtgtttatat tgcgaacctt ggaaaataca atgaaggtga gttggtgggg gcatggttta
      121 ccccacctgt cgattttgat gaagtgaaag aacagattgg attaaatgac gaatatgaag
      181 agtacgcgat tcatgattat gaactgcctt ttgagattga tgagtatacg ccaattgaag
      241 agattaatcg gttgtgtaac ttagcggagg aactagaagg aacgccaatt ggtgaagctg
      301 cttctgagat tcaacacgct ttcttcaatt ctttcgagga aatggtagag catgtggatg
      361 atattgtcta ttatccagat tgcgatgata tgagtgatgt ggcgcattac ttgattgtag
      421 aagctggtga ttttggcgaa gttccagaac aattgaagcc gtatattgat tttgatgctt
      481 gcggtcgaga tttggagatt ggaggaaatt atttggttac tagtcgtgga gtttttgagt
      541 atccagaatg aatcaaacac tatatatagt gtttgattag tatatgtaag caaaaaaaca
      601 caatatatag agtgcaaact gaagggtaat gtgatataat atttgccgaa gaaatatttt
      661 tgaaatgagg taatattatg gcaaatattc aaacaagttt tattgatttc cataattcaa
      721 ttagattgga cgtagaagac aatacgctac ttaaggacta taaagaccaa gtaattgatg
      781 gattaaaaga ttacttacct gatgatgtga agtttgaaac ttttttacag ggaagctatt
      841 ctgtttatac cggaataaaa agctgtgatg aaaaaattga ttttgacatc gatattgcag
      901 ttgctttcga aattgatcat actgtttatg aagatccaag ggagcctaaa ctgtgggtaa
      961 aagaggcgct agttgaaatt tttcctaatg ctcaagttaa tttaaaagta ccttgtgtaa
     1021 ctgctacatt tactggaaaa aagacaaaaa aaaatgtgca cgttgatgtt gcggtttatg
     1081 ctaaagaaga tgaaaattat ttcttagcaa aagcaaaaga attcagtgct cccgaaaacc
     1141 gatgctggga ggaagcggat cccaaagtat taaaagagaa aattaatagt catgtagcag
     1201 attcagatga tcgaaaacaa tttcgaagat gtattcgcta tttaaaaaga tggaaagata
     1261 acaattttaa ccaagaatat aaaccaacag gaattggact tacaattaat gtgatggata
     1321 cgttcctagt taataagtcc actgattttt taactagaaa agttcagtat aatgatatgg
     1381 aatgtatgaa gcagattgtt tcttcgctga aagattcatt tgtgtatgag tacagtgaaa
     1441 cagatggttg gcattatcgt ctacatgcat atcttccagt gaagcctaac agtgacacct
     1501 attcaaaaat gacagtgaat cagatgagtg attttaaaaa caaactttct aaattatatg
     1561 atgatttgat tttcgctatc gatactgaag atgagtatga agctacaaaa cgattaaata
     1621 atcaatttgg ggaggatttt ttaattattt cagaagaaga agttacagaa aaaaacttaa
     1681 gaaatgcatt tgttacagac tatccgagtg cttaggttgg tgttaaagat tggattatac
     1741 tgatatttta aaagaaaagc taacagatgt tgtgcgttta ttcgatggta attactatgg
     1801 ggatttttca tttgctacaa ttggaaagtt gcgtttaaaa attgaaatac caaaacattt
     1861 tccattggta cttcctagga tttacattca taattttgat catttaggac tttacctacc
     1921 acatgtagaa aaagatgggt tggtatgttt ttcttcagat ataaattatt tagtagatga
     1981 agaaaattca gaattggttt tattgcagtg tgttaataaa actataaaaa caattgagga
     2041 aggtttgaaa gggttaaata aaaatgattt tcgaaaagag tttgttgctt tttgggacaa
     2101 acaagagaat caaatttcgt tattctacct ttgtgaacca gcgttgttta tcaagtcagt
     2161 tgatacaata atgtacggta agtctttggt tgctttggat aaagagaacg aattacaatt
     2221 aaatgcagcg agtagattgt ttaatggaga gaaagaagat actcatgaat tagatgcata
     2281 ctatgttcca attagaaaca agaataatat tatgcccccg aagaataata aagcatggtc
     2341 aaagaaggat attttacaaa tagtaaaaaa aaatgtaaca ggtgcaaata aaagattttt
     2401 tttaaaactt gaaaaaagaa agaatagaca taatgtttta cttattctag atatcccaat
     2461 tgaagaaaag tcaaataatg tcataattgg atttcttttt acaaaacaat ctggatctat
     2521 aaaagattat aagggtacgc ctaggactgt attagttcag cgtcatgatg aattatattt
     2581 ggtgaataga acgagcggag aaaactatgg attgggtgct aatatagcta ttgttggggt
     2641 agggtcagtt ggcagtaaaa ttgctgaaaa tcttggtcca ttaggtgtga gaaagatgac
     2701 actcatagac aatgattgtt ttcaagctga aaatttgtat cgaaattcac ttggtgcaga
     2761 tagtttaaaa aatgcccgat ctcagaataa tttttttaaa gttgatttaa tgcaggagga
     2821 gctcgaaaaa aaaatcccat acctttgtgt tgaagtggag tcaatgaacg ttttagactt
     2881 aattagtatg caacctggtt ttttcaagga atatgactat gtttttgttt gcataggaga
     2941 tacaatggca agcctggctt taaataatta tttttccaaa agtagtcaaa aagttttcta
     3001 ttcttggctg gagccatatg gcattggggg acacactcta tttataaatt attctctaaa
     3061 tcaaaaaggt tgtttcaatt gtctttatac agattcaaac tctaaaaata ggtgttcaaa
     3121 tagagcttct ctggtaaaag aaggacaaat ttttgaaagg actttagcga gttgccgctc
     3181 aaaatttata ccatataatg ctattgcctc gacggaatgt gcaattaatg caataaaact
     3241 tttccataaa gtcaatcaaa ataatatgac caatagtact cttttttcat ggataggaga
     3301 tgggagtact tttgagaatt taggatatca atatagcgac agaaatactt ttcttaatgg
     3361 agtcaataag tttgaagaaa gattcttttc taaatattgc aaagtatgtg gggagttaga
     3421 tgattaaatt ttcgtgtgac cgtattgtca ttgaagtaag ggatgaagtt cttaagatat
     3481 ttgaacaata tcggcaggga aaggatgact tgcctgaagc tggaggagtt cttcatggaa
     3541 aaagacttaa gaatgggagc ataataatta ccgatataac aacacctcaa ataggagata
     3601 tacaaaaaag attactcttt aaaaaaaacc gagaaattca tcaacagctt tctaatgaga
     3661 aatggataga atcaaataag ttttctattg cattgggaga atggcatact catcctgaag
     3721 ctgtaccaac tccatcaaat gttgataaga aaagctggag actgaacgtg agcaagcaac
     3781 atgatgatag agtttatgtt tttattatcg ttggattttc tgatttgaga atgtggttac
     3841 tgtcaaaaaa atcagatatt aggcaggcta taaaactata aaggagtgat taggtgaaat
     3901 cacaaaagaa tatttggtgg tgggggtttt tagtaggaaa ggtaatgata ccagcccttc
     3961 tcttaatttc tctaccgatg attacttttt tctttaccaa taaaaaaatg agtaaagaag
     4021 ccgtgatttt tttctttgga gaccaaaaag ctacctttat tgaaacgtta gtaacatctc
     4081 ttcagcttaa tttgaattac attttttcaa ttattattgt tatatcgtta ttgaattttt
     4141 ttaaaaaaag aaattcagga aaggtgttta actctaatgg aaatgtgtac tacaactatt
     4201 tttattttgt attttgggta gcagcaactt tacttggtta tgataaaatt cagattgcag
     4261 gaattcctat acacatgcaa tataagttgg ttttgagtgg aattttttct gaggtgttgc
     4321 cagatatata cgatgatcat tacgacagtg atggaacatg taaagtaagc attgaaaaag
     4381 aaaatttcga tgacatcgat ggatatgact ctgtgaactt actgattatt gatacttacg
     4441 atataaaaat gagtgaatta tctatggaaa atcaaacata tcctacgata attgttagag
     4501 ggaattcaat agatggagtt cgtaaagtta atagatcttt aatcttggaa ataaaaaaaa
     4561 caatggatga aattcaaaaa agtgacttta aaaaagtatt tgttgcttca acatctaatc
     4621 caaaaaatag tatcaatatt attaattcat cgtttagatt tttcggtcga tcacgtaggt
     4681 ttaaacttta tgttcttcaa aaggattatg caagcaatgg aaagtatagt aagaaatata
     4741 gaatttttat ttaagaagga atttttcctt ctttttattt tggaggaaac tatgaaaaaa
     4801 ataaaaagct acaccagtat ttgggcagtt gaaaaagtca tttatgcgat taatgacttt
     4861 cagctgcctt ttcctgtgac ctttaaccag atggcttggt ttgttttatc cttgatgttt
     4921 gtcattgtat ttgcccatgt gcctccgcta tccatgatag agggggcttt tttaaaatac
     4981 ttgggcattc cgatagcggt cacttggttc atgtcgcaaa agacctttga cggtaagaaa
     5041 ccattgggat ttttacgatc ctttatcagc tatcacttac ggtttaaggt cacttttgca
     5101 ggaaagaaag ttaaagaaca gaagaaacga tgggacgaac caatcacctt ggtaaggagt
     5161 gtgaattatg tacccgatta agtatattga aaataatctt gtctttaacc aagaaggcga
     5221 gtgttttgct tactatgaat tagtgcctta caattactcc tttttgtcac ctgaacagaa
     5281 gtatcaagta catgataact ttcgccaatt aattgcgcaa aatcgtgaag gtaagatcca
     5341 tgccttacaa attgccacag aaagtagtat tcgtgccaca caagaacgtt cgaaaaagga
     5401 aattaccggt agactcaaag aagtagcaaa gcaacgaatt gatttacaga cagatgcatt
     5461 agtatccatg attggcgata gtcagattga ttatcgcttc tttattggct ttaaactgat
     5521 ttcgacagat gaagaggtca atctaaagaa tctcaggaaa tctttctttt ctggacttca
     5581 agaatttgtc tatggagtga atcatcattt gatgggtgat tttgtttcct tatccaatga
     5641 agaaattcgt cgctatacca agcttgaaaa actaatggaa agtaaattgg ctcgaagatt
     5701 taaagtcaaa cgagtaacgc cgagtgactt gacgtattta attgaacata tctatggtga
     5761 aaaaggcaca ccgtttgaag aatatgaatt ccagctacca aagaaaaagc tgaaatcaga
     5821 aacgctggtg aaacgttacg acttattacg tcccactcgt tgtttgattg aagaaaagcc
     5881 tcggtactta cggatggaac atgaaaatca tgagtcttat gtagcgtact taacaattaa
     5941 taccattgta ggggaaatgg agtttccttc ctcggaactt ttctattacc agcaacaaca
     6001 atttacgttt ccgattgata cttcaatgaa tgtggaaatt gtgacgaata aaaaggccct
     6061 agctaccgtt cgtaataaga aaaaggaatt aaaagattta gacaaccatg cctatcaatc
     6121 tgataatgaa acgaactcca atgtattaga tgctttggat tccgtggatg aattggaaac
     6181 caccttggat cagtcgaaag aatccatgta taaactaagc tatgtggttc gagtgagtgc
     6241 taaatcagtg gatgaattaa aacgccgctg tgatgaagtg ctcgattttt atgacgatac
     6301 caatgtaaaa ctagtccggc cctttggcga tatgatgggt ctacacaaag aattcttacc
     6361 ctcaagtaag cgatacatga atgactatat tcaatatgtt acgtctgact ttttggcggg
     6421 acttggtttt ggggctactc aaatgctagg agaattagaa ggcatttact ttggctataa
     6481 cgtagatacg gggcgcaatg tttacctgaa accggcattg gcatcacaag gggtcaaagg
     6541 ctcagtaacc aatgctttgg catctgcttt ccttggttcg ttaggtgggg ggaaatcttt
     6601 tagcaacaat ctcttagtct attatgccgt cctttttggt gggcaagctg tgattgtcga
     6661 tccaaaagga gaaagaggcg gttggaaaga aacgttgcca gaaattgcgg atgaaattaa
     6721 tattcttaac ttgaccagta agccagaaaa tcaaggctta cttgatccct atgtgattat
     6781 gaagaaaaag aaagactcag aaagtttggc gattgatatc ctgacctttt taacagggat
     6841 ttccagtcgg gatggggaga agttccctgt gttacgcaaa gccattcgaa cggttaccca
     6901 aagtgatcag cgaggattat tacaagtaat cgttgaattg cgtaaagagg actcaccagt
     6961 cgcggaaaat atcgcggatc acatcgagtc ctttaccgat tatgattttg cccatttgct
     7021 tttctcagat ggttcgatta cccagtcgat tagtttagat aagcagttaa atatcattca
     7081 agtagcggat ttggttttac cagatgcaga aaccactttt gaagaatata ccaccatgga
     7141 attgctgagt gtttccatgc taattgttat ttccactttt gccttggact ttatccatag
     7201 caaccgagaa attttcaaga ttgtggacct ggatgaggct tggagttttc ttcaagtagc
     7261 tcaaggaaaa acgttatcga ataaattagt gcgtgctggt cgggccatga atgccggtgt
     7321 ttacttcgtc acgcaaaaca gtgatgactt gttggatgaa cgtttgaaga ataatattgg
     7381 gctaaagttt gcatttcgca gtaccgatat tcatgaaatc aagaaaacct tggaattctt
     7441 tggattagat aaagaagatg aaagtaatca aaaatgcatg cgagatttgg aaaatgggca
     7501 atgcttgatg caggatttgt atggtcgagt aggtgtggtc caagttcatc cagtctttga
     7561 agaacttttc catgcttttg atacccgtcc gccagttcaa gagagaatgg agtgaggaga
     7621 tgaaaaagaa actttttcgt atctttggaa ttgttttaat cagtttggtc actctgataa
     7681 tacttttgag cttagttgga acggtagctg aggcaacagg tctggttgat gatacagttg
     7741 gagccggaaa tctttattcc caatattcac ttggtaacta tcagttggat ttctttgtgg
     7801 atagttcgtg ggattggctg ccttggaatt ggggtgatgg cctagggaag agcgtaatgt
     7861 atggacttta tgccattact aattttattt ggacagttag tctctactta tcaaatgcca
     7921 cgggctacgt ggttcaagaa gcctataagc tggatttcat ttcagatacg gctgaaagta
     7981 ttgggaagaa tatccaaacc ttagccggca ttaccgaaaa tggactccag acctcgggct
     8041 tttactttgg gtttctatta ttgatgattt tagctctagg agtttatgtg gcgtatacag
     8101 gattattaaa acgagaaact accaaagcag tccgggctgt cattaatttt gtgatgattt
     8161 ttctactttc gggttcgttc attgcgtatg cgcccaccta tatcacaaaa atcaatgatt
     8221 ttagctcaga tgtcagcgaa gcggctctta gccttggaac cgagattgtc gtgccgaatt
     8281 cagaaagcca aggaaaggac agtgtggatt taatccgaga tagcttgttt tccattcaag
     8341 tccaacaacc gtggttactc ttgcaatttg atgattccaa tatagaagaa gttggcgaag
     8401 atcgtgttaa caaaatcctg tctgtaagtc cagatgaaaa taaaggcaag gatcgggaag
     8461 aagcggtcaa agcggagatt gaagataatg acaatgcgaa tctcagtatt accaagacca
     8521 tgaaccgctt agggaccgtg gtctttttgg tgttgttcaa tattggcata tccttctttg
     8581 tctttctcct aacagggatt atgttgttct cgcaaattct ctttattatc tttgcgatgt
     8641 ttttgccgat tagtttctta ttgtccatgt tgccgactta tgagagtttg ggcaagaagg
     8701 cgattattcg cttgttcaat acgattatga tgcgagcagg agttactttg gtgattacga
     8761 ctgcatttag catctccacg atgtttttca atatttcggc aacctatcca tttttcatgg
     8821 ttgcttttct gcaaattgtg acctttgccg gtatctattt caaattgggc gacattatga
     8881 atatgttcaa tctccagagt aatgatagcc aatcaatggg aagacgggtc atgcgaaaac
     8941 cgcaaatgtt aatgaatcga acattgcggc aactcaatcg aaatgtggga cggactttgg
     9001 cttttggtgg tggtgcagca gttggaaata agttggcaaa ggaacaatca aaatccaaat
     9061 ttaagccatc cggttcttct ctacgaaaga attcacgatt accgaacgat cacgagatac
     9121 catctgactt gaataaggaa aagccaattc caaataataa gaaacaatcc cgaatgaatt
     9181 tggccgggag aaaagtcggc aaggttctgg atacccaagc tttggtgaaa gataagacca
     9241 aacaagtaaa ggatggagta cggaatacgc caaccaactt gaaatacaac ttccataaag
     9301 gaattgaaaa aacgaaaaaa gcaccggaag agtttaagcg aggactggtc caagaaaaag
     9361 caaatcgagg agagttgcga gaaaaacaac aacaacgtag agacgaaaaa atagctgaaa
     9421 agcgcaaagt attgaacgaa atagggaatc ctcatgggaa aagaagggaa aatgttccga
     9481 taaaagcaaa ggtccaatct caaaaggata gagcaccaaa gcggattgtg agagcaaatt
     9541 cgaatcccga aatcaaacga aaattcgcta gccaagaaat catttcaaaa ggaataaacc
     9601 aatcctcaag taagaaaaat tcttttcaac aagtgaaacg gcggtctact ctgtccaaac
     9661 gaaccaatcg aaaagtacaa aaaagtttga accctcatcc caaaccaagg tcaggtgaga
     9721 aaaaatgaac gtaaagaaat taagtgccat ttcgttaact ttcctaacaa tctcttttat
     9781 gggtatcttg atgtgtgtgg gcttattgtt tggtgaagac agtgaaggaa gtagtagttc
     9841 aggtgctcct cctgggggga gtagtgtctc agaagaggtt ttgaaacacc gtgcaatggt
     9901 ggaaaaatat gccaaagaat cggatataac agagtatgtt ccgattcttt tagcgattat
     9961 tcaagtggaa tctggtggca caatggaaga tgtgatgcaa agttctgagt cagccggttt
    10021 accaccgaat tcattaagta ccgaggcatc catcaaacaa ggatgtctct attttgcgtc
    10081 tttggttaag cgttccaaag aattaggttg tgatcaagat agtatcattc aggcctataa
    10141 ctttggcggt ggttatttgg actatgtggc aaaaaacggg aagaagcata gttttgcttt
    10201 agctgaatct ttttcaaagg aaaaatcttg cggtcaaaaa atcgattacc ctaatcccat
    10261 cgcgaataag gaaaatggtg gatggcgcta taactatggc aatatgtttt attgcttatt
    10321 ggtgaagcaa tacctaacta caacacagtt tgacgataaa acagtccaag ggatttttga
    10381 cgaggcgttt aagtatgaag gaacggctta tgtatttggt gggtcaagac ccgaaacagg
    10441 atttgattgt agtggcttga ctcaatggtg ttatgccaaa gttggtctga aacttcctcg
    10501 ggtggctcaa gatcagtatg atgccatgac ccatattgac ctaaaagatg ccaaaccagg
    10561 ggatttggtt ttctttcatt cgacttatga tgccggtact tatgtgaccc atgtcggtat
    10621 ttatgcaggg gagaatcgca tgtttcatgc aggagatcca gtaggctgga cgaatctaac
    10681 agaaagctat tggcagcagc atcttatcgg tgctggacgg tacaaataag agaaaagagg
    10741 aaaaagaatg aagataaaaa ttgaacggaa tcaaaaggaa aagagcctaa aaaagaaaaa
    10801 agaacgagtc gtatccgtgg gtaagcatcg aaaaatggtg cttgcccttt ggcttttatt
    10861 atcttgtagt cttacttttg gcgtttataa aaatttcacc gccattgatc aacatacaac
    10921 tcatgaaaaa gtggttatta aagagaaagt agtgaatact tctggtattg aaaactttac
    10981 gaaggatttt tcgaaagaat atttttcgtg gaaaaataac aaagaagcaa ttgaaaagag
    11041 aatgaacaac ctcgaacagt accttacaga agaaggtttc gctttaagtc aagatatggt
    11101 tcgagcagat attcccacta attccgaagt tcagtcagta aaagtcttag atgttgaaaa
    11161 gagctcggag gaatttgtcg tttcattttt agtggagcaa aagattacag agggaaaaaa
    11221 ggcacaatcg atttcttctg cataccgagt gacgatcttt gaagatgaaa acaggaatta
    11281 tatagtaacg agtttgccaa ctatgattgc aaaacctgat agggcaaaat acaagacaaa
    11341 acaagtagaa aatgattcga aaattgatgc caaaactaca gaagaaatcg ctgagttttt
    11401 ggaaacgttc tttaaattat atccatcagc ttctgaaaaa gaattggagt attatgttga
    11461 aaatgatgcg atgcgaccaa tcaatattga cttaaagttt tcgaagttag aggatccagt
    11521 ttacctacaa aacggaaaaa aaattctagt gagtacaaag ataaaatacc ttgataatgt
    11581 atcaaaaacc actagctact ttcagtacaa attaatatta caaaaaaatg gaaactggaa
    11641 aatcattgat gttaaataat aatgactcaa tctaaattta tgagaagtta taagtagttt
    11701 aacttatttc aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccgttcggta
    11761 actcgaaaac cgacaattaa gacatagtgc ctttttattt taatccatgt cataatcagt
    11821 ctatataaga aatggttgat ataccatgga attagataca aataatcatt cattatttct
    11881 tctttgttac caccttattt tagtagtgaa acattgtaaa catctcattg atgatgtaat
    11941 gttagcttat gcaaaaaact atctttgaaa gaatatcaga atcacatcac attaccttag
    12001 ttgagtgaaa tcgtgataaa gactactttc atattatgct caaagcacaa cctaaaaccg
    12061 aattaacaaa attcagcaat gcctaagctc atgtctgata aaatgagact ttctaagggt
    12121 caaaaatttc ctttgaatac gatcattagc ttagataaaa gtaaaaacct tagaactacg
    12181 gggatagctt ggttaataag agatatcgct gttaataaag acataagctt gcaagtatgc
    12241 tctattccca agaatctccc acttcaagca ctaaggatcg ttaaattcag ctaagtggca
    12301 agtagttcac tagacgataa ttatctccat atttcaatct cagcttttcc atttttcata
    12361 gcaaattccg ttatttttat agcatctaaa agattcagct tttccactga tctttcacca
    12421 cttttcagct tttttattgt gcttaaattg ataccagtac cttttgatat tgaattggaa
    12481 gtaaaatcat taagggtttt ttttattaaa ttcatatccg caattccagc gttcataaga
    12541 atgaagattc tcctctcata gactactttg aaaatggtac tacagtggct gtttatatat
    12601 ttcagtaggt tactgtagtg gtcattttat tataattaga aaaggaacct aaatcaaaaa
    12661 tgctaaagaa aattttcata gtattaaacc tatcatgact aaagccacgg aattcttgat
    12721 aaaagagtat acttgcaagc ctatgtcttt actaacagcg atatctctta ttaaccaatc
    12781 tatccccgtt gttcctaagg ttcttgatgt tttctaaggc aatgcttgta tacatagccc
    12841 ttgatcaaaa tattgatact aacgttgacg tttcgatcat gatgtgtacg gcaaatagga
    12901 caagtccatt ctcgaattgt gaatgacttc ttaccatctg tgtgtccaca ttctgaacca
    12961 atttgactag acggaaatca tttatctatc ttgatgattt ttttgacaga agtatcgaaa
    13021 atctttacat aatctgggtg ttgcattctt atgcagatga gagcgttagc tccgtattca
    13081 taaccagttg cctctaaaac gtagtccatc ggtggatacc tagcttgcgg ttgccagtgg
    13141 ctttcgcagt cttcttgtaa tcaaatcgag catctaattg agtactagta ttttgaagat
    13201 aatacttgag tttttcggtt ttcagaagtt cacttcatct tccatcatcc atggaaatcc
    13261 cactcaccca tacgcaggcc tccaaaaatt cttcaacaat ggtttttcag acatttttct
    13321 gaatacctcc ttgttttttg atacgccaga tagtcaggaa aaaggcaaca aaacacaaaa
    13381 caaagctgcc taaaaaggta atcttcattc catataaaaa gacatccggc cgatctgcga
    13441 tatagttagt tactcgttgg cccatcttag cagacatcgc ttgatataag accgtcgtag
    13501 aaagcgaaat cccgatgacc atccccaaat ttcgggcaaa ggaattcatg gagcctgcca
    13561 gtcccaaatt ttcgatagtg acagaactca taaccatcgt attatttggg gactggaaca
    13621 aggcattgcc ggcacccatg atggcagtag cgacaatgta cagccacaaa ggagagtcca
    13681 ctgacaaaaa catatacatg acttgggtta ccgacaataa acccaagcct actaatacaa
    13741 aaatctccgg gccaacccgg tcggtcaggt atccgcttaa aggcgccccg acgaccatca
    13801 tcaagggaaa gaccatcatc aacagtccgg catagctagg actcaacccg cgagcatttt
    13861 gcagataaaa aggaatcact acattaacaa agaagttgga agaaaaaatc aacaaggctg
    13921 taatcaagct catggtaaaa atcttgttct taaaaatacc gaaagaaatc aatggagagg
    13981 ctgctttctt ttcaactttt aaaaagctga caaaggcaat cactgaaatt acgaacagta
    14041 caagggggac tggatggaaa aagccacttt cttgccccaa aaagattgtt ccaaaaaggc
    14101 tgataatcgt agccgcaatg gcagcaaagc cccctccatc gattgtatgg ccgctcatgg
    14161 tgatatcttt tggtaagaat ttttcagcaa tcaacatcgt tacgattccc accggtacat
    14221 tgatccaaaa aatgtacgcc caagaaaatt gggacaaaat caagccgcca agacctggtc
    14281 cagcaatcga gcctaatgaa acaagcgcac cgattgctcc caatgcccga ccacgttcat
    14341 gtatgggaaa gacttccgtg atgattccgg catttgtagc catagtcata ctagcaccaa
    14401 tggcttgtac cacccgtgcg aacaattaaa tacctagcga atgattgaac ccacacaaca
    14461 gcgagcccaa gacaaagatc agcgtaccaa tccgaaatac tttgattttc ccacagctat
    14521 cgccaatttt accaaacagc aacaaacaag aacagacaat catcagataa acggacacta
    14581 cccattccgc ttgattcatt ggtatgccca tatcatctga gatggtaggt agtgcaatat
    14641 tgacaatact gccatccaat gtggacatga aggtaaacat agatatagca atcaaaatcc
    14701 accagcgatt tttttggact tttgcatctt cttgataact atgcataata ggatttcctt
    14761 tctaatgatc gtgtagctaa gaacaatata acctatgtct actgcccaac aacttctcgc
    14821 gactctagcc ataggaagct tgagtaattt cgatttgtta ttctcatgta accatgtaag
    14881 tttgagatgc tttatttgaa cttactctat agtaactagt gataattcgc tttactttct
    14941 tctagcaatt tctaaaagtc gacttcattg ttcttcagat taatagtgtg agctatctcg
    15001 tgagacgtaa gaaaatttcc attctacttt ttcccaattt tttcatattt atggtgttgc
    15061 acctatttgt tttccaattt gcttttcaaa tacattgcta tgatattcaa ttccatagga
    15121 ttatactaaa tgtcaatagt atgaataata tattatagat gtttatatat cactgttaat
    15181 tattatccac aataattaat gattacctcg atatttgtca atcaaaggtt gaaacaattt
    15241 attttcagca tcttttctaa cttgagctgc actcttaata gaatcatacg atcctaaata
    15301 gtacctcttt tttttaaagt tatatacgca atccatttct ctttagaacg ataaactccc
    15361 ttaacaccac ttgtattatt tttcggtaat tttgacgtta gacactctat tactgtccct
    15421 tcaacacatg tccttgtttc ttgccttttt aaacatccac aactttgagt atgtccaaaa
    15481 aataagttac tataacttat ctcacacata ctgccacact cacattgaca aacccatttt
    15541 gttttccatg aacagttatt gtccaaagga tactttgcag taagtaatcc gaatttttta
    15601 ttttcaatgt gatgcttttt tgatttcaaa catccacagc tttttacatc accttttaga
    15661 agtcgctttg ccgatactaa cttttttctc ccacacagac attgacattc ccaaacaaca
    15721 gttccttcac ttcgcagttt agttggcttg attgcaacta acttaccaaa agttttattt
    15781 gtcaaatttt tatatttatg cctattttta taatttccac atccacaact tttggttcta
    15841 cctgatagta agtttgatcg atatacaatc gtttcattcc cacagtcaca taggtaacga
    15901 tattttttat atccattatg agtaatctcc tcaacaacag taaggtgacc atatctttcc
    15961 ttcactgcac ctaccctccc aagaaagaag taccatataa tttcaattaa cttttaataa
    16021 atcagaaaaa aatattttca atttttctta gtctgatttt atgactaatt tataatacaa
    16081 gactgaacga tacgtacacg aaatcctcaa gatagataaa ttgttttcat ctagtaaaac
    16141 ctgctcaaaa tatgagcata aagacggcaa taatacactc gatattcgat aatggacttg
    16201 ttttatttgt caaactcatc gtaatcgaga tatcaattcc agtctcaata tcctgatcaa
    16261 aggtctaaga atccaaccat tttcttgtta ctttgtcaga ttgattacct tttttagtct
    16321 ccgaataata aggttttcgg atcaactaac atgctctgag aaagactact atctttgcaa
    16381 atttccaaat gaaggtgtgg gccagtacta ttcccagtac ttcctacata gccaattatt
    16441 tgtccttgca aaacattatc tccagttttt actaggtatt gttgctggtg agcatataac
    16501 gcggttgatc catcttcgtg ttgtatcacc acgtaatttc cccaagagta gtgaaactct
    16561 gctttaacaa ccttaccagc tttaactgca tgaatcggct caccctgtgt agctgccata
    16621 tcaagtcctc ggtgaaattc atttccacga tttccaaaca aactggttat tgaataatta
    16681 tgaataggca cttggtatcc agtcttactc atttgtggac ctttaacttc tttatcatag
    16741 ttggttaaat cataggtttt aattaattca tttagcttca aatagtattg tgtatctgtt
    16801 gcataccgtc ctgtaaggaa tttagttgct tctcgatatg ttttactatt agcttttaat
    16861 gctcctgcat aaaaatcttt atttccacta attcccttag ttaataattt ggcataatct
    16921 tcaaaacttt cctcatagtt ttcatacact ctaaattttg attgggtcgt ataaagcgtt
    16981 ccattaccca aatcttcgtt tgttaccata atgacaaagt ttccattata agttcctttt
    17041 atcccaaata aattgtaatt cggtgcctga gctaactggc tttgaccact ggcagattct
    17101 aagatcgctt gtgcaatcat aacagaagca tataaatcat atttctgacc aatttttctc
    17161 gcagactcac caattttcat aataaaattt tctacagatt catttttgtc aaaatgaatt
    17221 tcttcattac cagatagtat tggttgtgat tggaaagatt ctgttgattc tatttgctgt
    17281 ggtgtcgaaa aattttttgt ttgtccttcc gtttttggcg cttccttttt tgaagactct
    17341 atatggtttg gtcttgtcac tttcgaagaa gttatttcac ttgtactact tgaagttgtt
    17401 gaagattcgg cacttgaa
//
LOCUS       DAZKSR010000065        16758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000065 DAZKSR010000000
VERSION     DAZKSR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.065"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..292)
                     /locus_tag="ACG8XG_001963"
     CDS             complement(<1..292)
                     /locus_tag="ACG8XG_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0773872.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADY"
     gene            complement(294..491)
                     /locus_tag="ACG8XG_001964"
     CDS             complement(294..491)
                     /locus_tag="ACG8XG_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0773873.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(885..2612)
                     /locus_tag="ACG8XG_001965"
     CDS             complement(885..2612)
                     /locus_tag="ACG8XG_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0773874.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2605..3798)
                     /locus_tag="ACG8XG_001966"
     CDS             complement(2605..3798)
                     /locus_tag="ACG8XG_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0773875.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3985..4629)
                     /locus_tag="ACG8XG_001967"
     CDS             complement(3985..4629)
                     /locus_tag="ACG8XG_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0773876.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4723..4857)
                     /locus_tag="ACG8XG_001968"
     CDS             complement(4723..4857)
                     /locus_tag="ACG8XG_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0773877.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4859..6991)
                     /gene="feoB"
                     /locus_tag="ACG8XG_001969"
     CDS             complement(4859..6991)
                     /gene="feoB"
                     /locus_tag="ACG8XG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0773878.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6988..7461)
                     /locus_tag="ACG8XG_001970"
     CDS             complement(6988..7461)
                     /locus_tag="ACG8XG_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0773879.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7862..8086
                     /gene="nrdH"
                     /locus_tag="ACG8XG_001971"
     CDS             7862..8086
                     /gene="nrdH"
                     /locus_tag="ACG8XG_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0773880.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8245..10404
                     /gene="nrdE"
                     /locus_tag="ACG8XG_001972"
     CDS             8245..10404
                     /gene="nrdE"
                     /locus_tag="ACG8XG_001972"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0773881.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10454..11419
                     /gene="nrdF"
                     /locus_tag="ACG8XG_001973"
     CDS             10454..11419
                     /gene="nrdF"
                     /locus_tag="ACG8XG_001973"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0773882.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11508..12647)
                     /locus_tag="ACG8XG_001974"
     CDS             complement(11508..12647)
                     /locus_tag="ACG8XG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0773883.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12956..13669)
                     /locus_tag="ACG8XG_001975"
     CDS             complement(12956..13669)
                     /locus_tag="ACG8XG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0773884.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13923..14624)
                     /gene="nagB"
                     /locus_tag="ACG8XG_001976"
     CDS             complement(13923..14624)
                     /gene="nagB"
                     /locus_tag="ACG8XG_001976"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0773885.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14858..16390
                     /locus_tag="ACG8XG_001977"
     CDS             14858..16390
                     /locus_tag="ACG8XG_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0773886.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16581..>16758)
                     /locus_tag="ACG8XG_001978"
     CDS             complement(16581..>16758)
                     /locus_tag="ACG8XG_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0773887.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5203 a         3450 c         2886 g         5219 t
ORIGIN      
        1 cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca ctcaaatttc
       61 ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca gtaaaatata
      121 gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt tgtttttcta
      181 attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga tctttactta
      241 tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc attttagcac
      301 ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat acaagtagtt
      361 gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt tccataatat
      421 tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga caaaattttt
      481 aaatgtttca taatctaatc acccctaaat taatgttttt agactattat tctaacattc
      541 attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct gaataagaat
      601 ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga gaatgcactt
      661 cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc tctttgactc
      721 gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga tgagctacgc
      781 attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca aatttggata
      841 tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt tttccagctt
      901 tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg atcttttcca
      961 ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa attgtctaat
     1021 actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt atggatctgc
     1081 tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata tttggcaaac
     1141 atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat cgcttgacct
     1201 agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt caagttgtca
     1261 tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc gattcctatg
     1321 attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat gtaggctggc
     1381 actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac ttgatcttta
     1441 tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat ggcatctgac
     1501 tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat cgttacatca
     1561 tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc tgtatcccat
     1621 tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact ccctatacca
     1681 atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg ttttttactt
     1741 ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg ctgtgtaaaa
     1801 cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc attcccgact
     1861 tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc gatcatagag
     1921 gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc catgatcgta
     1981 cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct cccgaatgaa
     2041 tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt aaaacgaacg
     2101 gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc gataccgaaa
     2161 aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa gtcaaggatc
     2221 ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa tggtacccca
     2281 atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat caaatcattc
     2341 catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt aagtcctgga
     2401 cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa aggagctgga
     2461 atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt ttgtaaaaat
     2521 gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac ccagtttgcc
     2581 actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg tttctgctaa
     2641 tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct ctacaactgc
     2701 aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca tgtctttccc
     2761 aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct tcctagcttc
     2821 taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa cagccatcag
     2881 catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat cagattcgat
     2941 attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg aacgatcgac
     3001 ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt cttcacctgt
     3061 tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa tccgtaaagc
     3121 ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt gcaggtccag
     3181 taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg cttcgtccat
     3241 cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt gttgcatccc
     3301 accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat tttctaatgc
     3361 tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta acccgtattc
     3421 tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa ccatattgat
     3481 tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat cttctccatc
     3541 aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga tcaatgtgga
     3601 tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta cgtcaaaatt
     3661 cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt tcggctggtg
     3721 atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg ttaaatgttt
     3781 tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt tctttctgaa
     3841 aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga aagaaatttg
     3901 cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt agccaagtta
     3961 atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt aaaaaaagtt
     4021 tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc ctgccgtaaa
     4081 tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg cgataactgt
     4141 ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat gcattgcttt
     4201 ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc gcttcgtttg
     4261 attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt gcttctccac
     4321 acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat attgcaatag
     4381 gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc gtacttccat
     4441 gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag aagcattgta
     4501 cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc ggttttcaac
     4561 gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga tttttttctt
     4621 tccagacatt ttctttcctc tttctttttt tactttccct catcttacta tgctctagca
     4681 aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct tgatctgttc
     4741 cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt ttaaacgagt
     4801 atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg tcgccatcct
     4861 attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt ggctgacgga
     4921 acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct aacgctacct
     4981 gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa taagctaaac
     5041 cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc cgatggatag
     5101 ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat gaataagctg
     5161 ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt tcggatactt
     5221 ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg accaatcctg
     5281 taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa acaactgcca
     5341 ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga gcataaaaat
     5401 tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt cctgcttttt
     5461 taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat tgaggaaaat
     5521 gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa cgtgtttttt
     5581 tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca gaaggagcta
     5641 cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc aactttgcag
     5701 aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg ttttcaatcg
     5761 tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata ggaatgaagg
     5821 attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg atcctcgcca
     5881 tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat tgcggtagaa
     5941 atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc tgcatccatc
     6001 ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat gtaccaaaca
     6061 atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc agataataga
     6121 tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt gcaatctgat
     6181 cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag agtgtacata
     6241 gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg tcgtccaata
     6301 gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact gctatctcgt
     6361 aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag cgtttcttgc
     6421 cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa cgtggatcat
     6481 atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc ttttcgatta
     6541 gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca ccaagaccat
     6601 aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc atcatattca
     6661 gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa agatttcgtt
     6721 ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga accaaataat
     6781 ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg ggtaggtcct
     6841 gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact gttacgcccg
     6901 gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta gttttcccgc
     6961 tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta tcaagattag
     7021 ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt cgattggatc
     7081 tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc ccatatccat
     7141 taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag tctctcctgc
     7201 ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat tgacaaagat
     7261 ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta aaatggcatt
     7321 gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga tattttgcaa
     7381 atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga aattctgctt
     7441 tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat gtattaaaat
     7501 cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa tcattctcac
     7561 atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg agcgaatctt
     7621 tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca caattttttc
     7681 tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt tcaaaaacac
     7741 aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt ttgacttttt
     7801 tactaagagt tttatactat ctattataga taataagaat gtgttagagg aggcactcac
     7861 catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt
     7921 tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc ctgaagcagt
     7981 agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg atgcagaaac
     8041 agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg actaaaagaa
     8101 tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg tcgcaaactt
     8161 ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc atcatcaaat
     8221 acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt cagttatttc
     8281 aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa taaggataaa
     8341 gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac ttttccatct
     8401 attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga attcattcaa
     8461 ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca acatttttct
     8521 ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa aaccaacgac
     8581 ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct ttactttgcg
     8641 aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca acgatatcag
     8701 ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt
     8761 ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa ctctgctttg
     8821 caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg tgaagccggc
     8881 gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat gaaattattc
     8941 gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg cgtggtttat
     9001 ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga aaatgcagat
     9061 gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa attttatgaa
     9121 ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga aaaagaatat
     9181 ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt tgcaaatcca
     9241 aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa
     9301 caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga gaatccaatc
     9361 gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca aacgccttct
     9421 gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg taacttaggc
     9481 tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg
     9541 actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa
     9601 aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt
     9661 gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa tatttacttc
     9721 atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa
     9781 gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta
     9841 actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc
     9901 ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat
     9961 caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca
    10021 agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt
    10081 tattacccag cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat
    10141 atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt cgatcaaggc
    10201 atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca
    10261 acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga
    10321 atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat
    10381 caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta
    10441 aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga
    10501 tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat
    10561 cccactttca aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt
    10621 aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga
    10681 tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt
    10741 catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa
    10801 agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga
    10861 gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt
    10921 tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa
    10981 caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg
    11041 aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga
    11101 aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta
    11161 tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta
    11221 taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa
    11281 tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt
    11341 ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta
    11401 tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga
    11461 gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt
    11521 tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa
    11581 tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc
    11641 agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa
    11701 ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt
    11761 tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg
    11821 acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt
    11881 ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag
    11941 ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt
    12001 tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga
    12061 gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg
    12121 gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat
    12181 aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt
    12241 tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt
    12301 gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga
    12361 ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa
    12421 aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata
    12481 taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat tttcaatatt
    12541 ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc
    12601 tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa
    12661 ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt
    12721 gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa
    12781 acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt
    12841 tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt
    12901 atttggctgg gcttattact tgatgtagga catctatttt acaacctcag atcatttatt
    12961 gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac tcctcgatca
    13021 catcggcata agtgagccct tttggaagat caatcgtata gatatttttg tacactcggt
    13081 agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt ttctcttcaa
    13141 aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt tctaactttt
    13201 tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca atcccgataa
    13261 aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat ccagcaattg
    13321 cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc
    13381 ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct tcatctgaac
    13441 gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca ctagcgatct
    13501 caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc gtacgcatac
    13561 ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat agcatagcga
    13621 tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc taaacaccct
    13681 ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt ttacctctta
    13741 cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa acggtgttca
    13801 atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta tttttccttt
    13861 gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc tactattgaa
    13921 aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc gtgattttgc
    13981 aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt gattgcatca
    14041 gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat tgaaccgatt
    14101 cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg tttgtttgca
    14161 ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga agcaccaggt
    14221 tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc gattggatgt
    14281 gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc ttttccatct
    14341 ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat gaaatgacgg
    14401 tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac agaagtcatt
    14461 tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg tgtgcttcct
    14521 gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat ttcaaatgct
    14581 ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat tgctccttta
    14641 tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat tagtatagac
    14701 caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag gtttcataag
    14761 atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca ctgtggacgc
    14821 tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga cattaaacaa
    14881 cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc aattaaatgc
    14941 tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat tgattaaaat
    15001 catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa atattcacaa
    15061 aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag aatttctaaa
    15121 aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt accaaacata
    15181 tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa cggaattaga
    15241 ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg ggttggggat
    15301 cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac gttcaaaggt
    15361 gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg aaccaaccaa
    15421 tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt ttgacggtgc
    15481 gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc atatcgcaga
    15541 catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat tgaaacaaaa
    15601 ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac aaatcgaaaa
    15661 gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg caaaaagccg
    15721 acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa gtaaagctgt
    15781 ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa ctgatttagt
    15841 catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct ttggtgaaaa
    15901 tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa cactgatcgg
    15961 tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa tcaattactt
    16021 ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt taagcagtca
    16081 atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag ggttagttaa
    16141 tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta aggtgaaact
    16201 tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga cgaaccatat
    16261 cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag gaacagtaat
    16321 catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca ttgagattga
    16381 accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa agttttgtca
    16441 caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt agctcaaaaa
    16501 acggcaaaat atgaggagct atttttattt ggctattctt attacttgat gcagaacagc
    16561 ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc tttaaaaaca
    16621 actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt tacacctaac
    16681 cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc tccaataaag
    16741 tacataaaca taaataca
//
LOCUS       DAZKSR010000066        16696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000066 DAZKSR010000000
VERSION     DAZKSR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.066"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..352)
                     /locus_tag="ACG8XG_001979"
     CDS             complement(<1..352)
                     /locus_tag="ACG8XG_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0773888.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFT"
     gene            complement(600..1436)
                     /locus_tag="ACG8XG_001980"
     CDS             complement(600..1436)
                     /locus_tag="ACG8XG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0773889.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1447..2376)
                     /locus_tag="ACG8XG_001981"
     CDS             complement(1447..2376)
                     /locus_tag="ACG8XG_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0773890.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2568..2921
                     /locus_tag="ACG8XG_001982"
     CDS             2568..2921
                     /locus_tag="ACG8XG_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0773891.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2995..3651)
                     /locus_tag="ACG8XG_001983"
     CDS             complement(2995..3651)
                     /locus_tag="ACG8XG_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0773892.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3877..5811
                     /locus_tag="ACG8XG_001984"
     CDS             3877..5811
                     /locus_tag="ACG8XG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0773893.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5897..7120)
                     /locus_tag="ACG8XG_001985"
     CDS             complement(5897..7120)
                     /locus_tag="ACG8XG_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0773894.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7114..8985)
                     /locus_tag="ACG8XG_001986"
     CDS             complement(7114..8985)
                     /locus_tag="ACG8XG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0773895.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9006..10124)
                     /locus_tag="ACG8XG_001987"
     CDS             complement(9006..10124)
                     /locus_tag="ACG8XG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0773896.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10207..11040)
                     /locus_tag="ACG8XG_001988"
     CDS             complement(10207..11040)
                     /locus_tag="ACG8XG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0773897.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11033..11949)
                     /locus_tag="ACG8XG_001989"
                     /pseudo
     CDS             complement(11033..11949)
                     /locus_tag="ACG8XG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(12176..13300)
                     /locus_tag="ACG8XG_001990"
     CDS             complement(12176..13300)
                     /locus_tag="ACG8XG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0773898.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13483..14391
                     /locus_tag="ACG8XG_001991"
     CDS             13483..14391
                     /locus_tag="ACG8XG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0773899.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14532..16145
                     /locus_tag="ACG8XG_001992"
     CDS             14532..16145
                     /locus_tag="ACG8XG_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0773900.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5287 a         3260 c         2765 g         5384 t
ORIGIN      
        1 aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg
       61 ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca
      121 tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat
      181 ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct
      241 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
      301 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
      361 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
      421 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
      481 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
      541 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
      601 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
      661 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
      721 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
      781 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
      841 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
      901 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
      961 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
     1021 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
     1081 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
     1141 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
     1201 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
     1261 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
     1321 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
     1381 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
     1441 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
     1501 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
     1561 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
     1621 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
     1681 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
     1741 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
     1801 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
     1861 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
     1921 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
     1981 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
     2041 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
     2101 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
     2161 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
     2221 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
     2281 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
     2341 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
     2401 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
     2461 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
     2521 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
     2581 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
     2641 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
     2701 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
     2761 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
     2821 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
     2881 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
     2941 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
     3001 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
     3061 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
     3121 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
     3181 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
     3241 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
     3301 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
     3361 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
     3421 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
     3481 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
     3541 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
     3601 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
     3661 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
     3721 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
     3781 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
     3841 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
     3901 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
     3961 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
     4021 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
     4081 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
     4141 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
     4201 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
     4261 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
     4321 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
     4381 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
     4441 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
     4501 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
     4561 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
     4621 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
     4681 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
     4741 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
     4801 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
     4861 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
     4921 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
     4981 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
     5041 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
     5101 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
     5161 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
     5221 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
     5281 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
     5341 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
     5401 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
     5461 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
     5521 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
     5581 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
     5641 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
     5701 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
     5761 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
     5821 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
     5881 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
     5941 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
     6001 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
     6061 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
     6121 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
     6181 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
     6241 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
     6301 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
     6361 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
     6421 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
     6481 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
     6541 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
     6601 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
     6661 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
     6721 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
     6781 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
     6841 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
     6901 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
     6961 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
     7021 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
     7081 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
     7141 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
     7201 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
     7261 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
     7321 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
     7381 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
     7441 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
     7501 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
     7561 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
     7621 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
     7681 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
     7741 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
     7801 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
     7861 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
     7921 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
     7981 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
     8041 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
     8101 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
     8161 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
     8221 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
     8281 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
     8341 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
     8401 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
     8461 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
     8521 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
     8581 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
     8641 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
     8701 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
     8761 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
     8821 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
     8881 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
     8941 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
     9001 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
     9061 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
     9121 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
     9181 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
     9241 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
     9301 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
     9361 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
     9421 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
     9481 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
     9541 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
     9601 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
     9661 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
     9721 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
     9781 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
     9841 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
     9901 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
     9961 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
    10021 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
    10081 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
    10141 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
    10201 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    10261 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    10321 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    10381 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    10441 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    10501 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    10561 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    10621 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    10681 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    10741 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    10801 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    10861 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    10921 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    10981 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    11041 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    11101 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    11161 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    11221 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    11281 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    11341 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    11401 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    11461 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    11521 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    11581 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    11641 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    11701 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    11761 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    11821 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    11881 atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga
    11941 ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact
    12001 tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat
    12061 agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag
    12121 tatttgatgt attttcattg attttattca atagttcata gtatttttgt cctccttagt
    12181 agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc
    12241 ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt
    12301 gtgccacttg tggatttttt gcatctttag ggacataaaa acttgatcct cctgatactg
    12361 caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag
    12421 atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg
    12481 cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt
    12541 cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat
    12601 ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt
    12661 ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg
    12721 ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa
    12781 atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg
    12841 tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat
    12901 atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca
    12961 cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca
    13021 attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca atttttaagt
    13081 caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca
    13141 gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga
    13201 tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa
    13261 tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt
    13321 caagttctta tataaaacgc tttcacttat gtatattaac ggaattagta acacaaatat
    13381 ttatataaaa aaatatttta cttgtcgttt tggcttttca tttttgttat aatagtcatt
    13441 aattataaat agaaagcttt ttatataaaa agagaaagga aagtggttct tttgttaaca
    13501 aacaaccaat cctccaatct tgtcaaaaaa acactaacct attccataga tacccatctt
    13561 catttatctg ctgctcccta ttttcgacta ctaataaatg aaagcgctaa tttatatgct
    13621 gaatttgcac ataaatctat agaactagat aatccttccc ttttattcat tgataaacag
    13681 caagaagtcc aaatcttttc tactcaaaag gaaacacaaa cttttatcat tatccagttt
    13741 tattttgatg agcaattatt agcacatatt acgggacaat ctttttctat agatcaagaa
    13801 ataacatcta tcattacttt tctgaaaaat gaacagttca aaattgatta tctaaaacat
    13861 agtaagccag ctatttccta tgatcaggaa atcaattctt ttttcatact atgccaacag
    13921 atgagcattc atttgctttc cttattagtt acttattgtc tgcaaaaaat tcaattgtct
    13981 tccttaccaa ggcattatca acaggaactt gctttattga caataccttc aaatgagaaa
    14041 gtcattccta aaaaaaagca tattctttat tcagatgaat tagttgatcc aatcatacga
    14101 tatttagaag aacatctttc agaaaatttt ttgcttgaac aattagccca aatttttttt
    14161 atcagtccag ccagcttaaa aaaacatttt agaaaagtga atggaaaaag tgttattagc
    14221 tattttaaag aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc
    14281 tatacagaaa ttgcagatat attaggattt cattcgatcc atcatttttc ttcagctttt
    14341 aaaaaatata cagggttatc cccttcaaag tattatctat ccttaaaata gaaaaaaatt
    14401 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
    14461 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
    14521 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
    14581 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
    14641 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
    14701 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
    14761 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
    14821 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
    14881 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
    14941 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
    15001 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
    15061 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
    15121 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
    15181 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
    15241 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
    15301 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
    15361 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
    15421 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
    15481 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
    15541 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
    15601 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
    15661 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
    15721 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
    15781 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
    15841 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
    15901 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
    15961 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
    16021 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
    16081 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
    16141 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
    16201 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
    16261 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
    16321 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
    16381 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
    16441 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
    16501 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
    16561 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
    16621 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
    16681 gttcctattt atttaa
//
LOCUS       DAZKSR010000067        16668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000067 DAZKSR010000000
VERSION     DAZKSR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.067"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..299)
                     /locus_tag="ACG8XG_001993"
     CDS             complement(<1..299)
                     /locus_tag="ACG8XG_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0773901.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSV"
     gene            1343..2146
                     /locus_tag="ACG8XG_001994"
     CDS             1343..2146
                     /locus_tag="ACG8XG_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0773902.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2731..3186
                     /locus_tag="ACG8XG_001995"
     CDS             2731..3186
                     /locus_tag="ACG8XG_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773903.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            3305..4363
                     /locus_tag="ACG8XG_001996"
     CDS             3305..4363
                     /locus_tag="ACG8XG_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFQ0773904.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            4405..6354
                     /locus_tag="ACG8XG_001997"
     CDS             4405..6354
                     /locus_tag="ACG8XG_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0773905.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            6357..9998
                     /locus_tag="ACG8XG_001998"
     CDS             6357..9998
                     /locus_tag="ACG8XG_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFQ0773906.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            10398..10736
                     /locus_tag="ACG8XG_001999"
     CDS             10398..10736
                     /locus_tag="ACG8XG_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773907.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10822..11067
                     /locus_tag="ACG8XG_002000"
     CDS             10822..11067
                     /locus_tag="ACG8XG_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773908.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            11087..13243
                     /locus_tag="ACG8XG_002001"
     CDS             11087..13243
                     /locus_tag="ACG8XG_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773909.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            13255..13488
                     /locus_tag="ACG8XG_002002"
     CDS             13255..13488
                     /locus_tag="ACG8XG_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0773910.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            13478..14344
                     /locus_tag="ACG8XG_002003"
     CDS             13478..14344
                     /locus_tag="ACG8XG_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773911.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            14359..16329
                     /locus_tag="ACG8XG_002004"
     CDS             14359..16329
                     /locus_tag="ACG8XG_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773912.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         6148 a         2354 c         3301 g         4865 t
ORIGIN      
        1 acagattgct gatgcaattt ttcaataact tgttttaatc ttgaatgttt tgaagaagat
       61 gaactagaag acatcaacgc aaataaagcc tttttagata ctttgacggt attattaaaa
      121 ggcggatttt tagggtcaag atgtcctata gcgtaaagat aaattttttg tggcatagca
      181 tccatttcaa caatgctatc tacaagttga ttcgataaag taacagttaa atcaaacttt
      241 tttagattgc tttcggtagt ttcaaatttc gggtttacat tcttttctgt tattttcata
      301 cgattgcctc ttctttctaa gcttggtacg agcttaaaaa cttggaactg gtacgattcc
      361 attgaataag tatagacctg ttttcgatac gttacaataa gaaatgtatc gtaaatttat
      421 aacataactc aatgtcagta aaacacattt tattttttcc aatcaaattt cattgaaaat
      481 acagcagaaa tcgctatttt agaacatttt caaaataaaa atccttttca ataaaatacg
      541 aaattataaa aattaccaaa attaaaaaac aaatattttt ctagttttta gaaataaatt
      601 cacatacttt ttcttttttt ggatatattc ggaataccac atcgcttttt gtccacgttt
      661 gcatcgcttt ttgtccacat tcacatcgct ttttgtccat gttcgcatcg ctttatgtcc
      721 accctaaaat ctatttaaga ttgatataaa gcctttttgg aggttgctaa agttttaaag
      781 attaaaatat atattataaa tataaataaa aggggaagct gaagcttcct ccttttcctt
      841 tttttcaatt attcaaagtt acacaagcaa aataaactcc tagatagtgt tagttttttt
      901 taagattttt tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata
      961 tctgttaata tatctgttaa atatctgtta atacatctgt taggggaggc ttaatctctt
     1021 gggagagtaa gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa
     1081 gtggacagta agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta
     1141 tcacactagg ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa
     1201 attagattag taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa
     1261 caagcagaaa aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa
     1321 aaagaagatt ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga
     1381 tgtagattta gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac
     1441 aacagctttg cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa
     1501 aatgaaacgt caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt
     1561 caaatacgac agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa
     1621 actaggtaat ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt
     1681 tcaaaaattc cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt
     1741 tgaatttatc ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac
     1801 aaaactaaga agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa
     1861 agagtggcga aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat
     1921 accttcaagt tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga
     1981 atttttagct cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat
     2041 ctatgcaaag aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc
     2101 aaacttgcca accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg
     2161 taattttaat acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg
     2221 caagaagttt ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct
     2281 atacaaattt taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt
     2341 tcttaatttg ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg
     2401 tgatatcagt tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt
     2461 ggacaagtca actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc
     2521 gtctgataaa tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa
     2581 atagctaaaa taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg
     2641 ctgattgatt tatttatgtt attatgatat tataataaca taaatgtgtt acgcccgaac
     2701 ctcattttaa aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt
     2761 aactatatgg agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag
     2821 catgggttac cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt
     2881 attaagctga gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat
     2941 gaactaaaag gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt
     3001 attggaaaat atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct
     3061 actccaatgg ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca
     3121 atagcaaaaa tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga
     3181 agataatttt tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg
     3241 aacctagaat taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa
     3301 actaatggaa aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg
     3361 gttgcttggt gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt
     3421 tctaggagtg ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta
     3481 taattattat ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc
     3541 aggagcagag atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt
     3601 aaaactcggt tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa
     3661 cttatatatg gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat
     3721 gttaagtact gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta
     3781 tcaagttttt attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga
     3841 taccaaagtc aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat
     3901 gaatgatgtt aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac
     3961 tcctgataat atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg
     4021 aagctttctt accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa
     4081 gaataatgtt gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa
     4141 aattgaagct acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga
     4201 ttcgcaagaa agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa
     4261 tgatacacaa aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg
     4321 agacatgaag acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat
     4381 tttagaggaa gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa
     4441 actgaattta atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt
     4501 gatgacgaac tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc
     4561 ttgttaggtg aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag
     4621 cgacaagatg gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg
     4681 ggagatgatg tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag
     4741 cgaattttaa aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat
     4801 aaagttgtta gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg
     4861 ttagcaaatg aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat
     4921 atcaaaagag atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa
     4981 agcgatgttg tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat
     5041 cctcaggctt cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta
     5101 caagcaaaca agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg
     5161 gaggatttga ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg
     5221 caaatatttg gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa
     5281 gaggaattaa agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt
     5341 aaacgtatga agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct
     5401 ctgtttacag gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga
     5461 ctttatggcg atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat
     5521 gatgcaatgc gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt
     5581 attgtgtgtg atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt
     5641 ttagatgaag gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac
     5701 actgtttcat tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc
     5761 gattcgttaa acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa
     5821 tatagcactg aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg
     5881 attggtgaag gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg
     5941 gctttatccg aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa
     6001 ttagtacgaa aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat
     6061 ggtcaaaatt tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa
     6121 gaggattcta aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa
     6181 gaagcgatta ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg
     6241 agtgaagccg gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct
     6301 tccgttgctg aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga
     6361 aattcaagaa atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag
     6421 ttattggagg agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc
     6481 ctgacaatca agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa
     6541 gtaaaccggc agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag
     6601 aagttaatga tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca
     6661 gtgccgattt cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg
     6721 caggtactca actagcatct actttagcaa cttatagtta ctacggatat attgaaacag
     6781 tctcaggaaa tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga
     6841 ttggtagttt tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg
     6901 aactccttgg aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag
     6961 gtggatcgga tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg
     7021 gattaaataa tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa
     7081 tactaggtta tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat
     7141 caccagtact tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg
     7201 caggatcgct attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata
     7261 taccaacaaa tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa
     7321 atcttgctcc atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa
     7381 tcaagttcgt tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca
     7441 atactagcag ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc
     7501 tattaaaaga atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta
     7561 atgtcgccga aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt
     7621 taggggatgg aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt
     7681 ggtcagcaaa tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc
     7741 ataactttga tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt
     7801 cggtagcttt attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata
     7861 atatcgctcc ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat
     7921 ggcgtacagc atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga
     7981 tgaacgctga atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca
     8041 atgtgaatat cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa
     8101 caggatcact tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat
     8161 ctctgatcgg actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata
     8221 ctgctgtggg attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat
     8281 ctgctatgat tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata
     8341 atttgattaa tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg
     8401 ctaagttatt atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag
     8461 caatcaagaa aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa
     8521 aagatatggt tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga
     8581 acaatatggg agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa
     8641 caccagaagg aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt
     8701 tcaaagattt agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt
     8761 taggacgtat ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag
     8821 atagtaaata cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg
     8881 atgaagatgg aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta
     8941 atattggtat ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag
     9001 atggaaatct tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata
     9061 gtaacggacg tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg
     9121 atgagtttgg taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag
     9181 tcgatgagga tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag
     9241 aactcgaaca acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg
     9301 agattacttt agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt
     9361 cagacagagt gcatcgggaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc
     9421 ttaacagcaa ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat
     9481 cgctaacaaa agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc
     9541 ctaatgttaa tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca
     9601 atcaatcaat tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat
     9661 ctacttcaga taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat
     9721 cagccgttga atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt
     9781 tagcaaaagc tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta
     9841 ttaaaatagg taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa
     9901 ctggtaaagt agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta
     9961 atcaaaaatc aactgaaaat aagtctgaag acgagtaaaa agaaggagca ttgatttgct
    10021 ccttcttttt actttgattt ctaactgaag atgtcaagga aaatttctat ttatgcaaaa
    10081 aaattatttt aaattgtcta taaatgaatt aaatatttac tgttcaaagt tgtataatta
    10141 aaaagggctt atgctggtaa agaacagaat tattatctaa acaaaaactt gataaaccag
    10201 catttttagc caataaaaaa agtgaaataa tttaatggaa aatctctttt aatcatcgaa
    10261 aatttatgtt attatgataa cataaagatg taaatatcga gacgtctttt tcttagttag
    10321 atcattgaca agtgaacaaa taagatttgt cgatgatata attaacttat taaaaatatt
    10381 aaaaaggagg tttttatatg ttcgattcta ttaaaaattt ggttagcgga atgacgcctg
    10441 atatgaaaac tttagtacaa gttgttttgt atttcttggc attcgttttt tcgttgaaac
    10501 caggattcac tgcaatgaaa ttttttggtg atgcaaaatg gggagatgga ttaaaatatg
    10561 ttggggcgat tattgcaatc tttattattc ctgtaattac aaccgttatg ctttacacaa
    10621 tgggtactaa aacaggtaat gatttgaacc aaaaagctaa tatcatatca gcacttattc
    10681 cagttgttgg tatctatcta gtttcaaaat ttccaaaaaa tgaactgtta ggttaaaagt
    10741 gaattgaaca aatttggata atatgccgat ttgttcaatt tactttttac caaaaaacta
    10801 cgctccaagc gtagtttaga agtgagggaa gaaagtaaat gtaagatggt ttttgataga
    10861 gaaaaaatgt tagctcacgc caacgaagta ttaatgagca gtctgaaagg taccgaactg
    10921 gcaaagataa tgaatatgaa tgttaaccag ttctatgatt atcgaaatgg gagtaaaaag
    10981 attgaaaaag ccagacttga aacgcttatt aaattcgaaa aagcatatgt atatatgcta
    11041 gataaacaga aaagaactat tgactgaaaa aaggagtttt acaataatgg caaaattcaa
    11101 agatattacc gaatctatta attatgtggc agaaacagtt cctgtagttg cactctcttt
    11161 gaagccacaa ttactcgaag tgtacaaaaa agaagaaaaa gaagcgaagg caaaaaaagc
    11221 taaagcaaag gtatggaaag aattcgatgc gttgctgacc aaagatttac caaagaaaaa
    11281 ttatatggta attgaagatg tacaaaagct caacagtgag ggagttatag atgaggatga
    11341 tgctattctc gattctgaga agtatgtcgt tctattgaag tcaagtttga attatgtagc
    11401 tgatctacca gaaaaattta aagagaagct attagcaggt gtccgtttta tggatagtag
    11461 tggtcaatta ttagttattc cggatgatcc taatgtgttt gaatcagaag ttgaagaagc
    11521 tacagacttc tatagcgcag tagagcaatt agaagtagct actaatggat tagaacgaat
    11581 tgctgagacc ttcaactctt ctgaagaagt tgaatttgaa gccgtagcag aaaatgtacc
    11641 agatgaaaaa gaagctcttt ttgtggatga taacaaacca accgaagcag aaaaccaaga
    11701 agtaagcata gaagaaacac aaatacaaac agaaacctac caacaaccaa aaattgatga
    11761 aaaacccgaa tcccaaagtg acaaagaaaa ttatgtttat gaagctgaaa aaatcacatc
    11821 tgaaagtgat gtaccgaaaa acttagatgc tgatttttta gaaaaaaatt ttgaacagat
    11881 cattgctgac aaatttcaac ctgtagtttt agatgagatt aatgaccctg tcgatttaaa
    11941 cgaggagatg ctatccatac ctgagttgaa accattgctg tttttagcta aagagagtat
    12001 gaacgctcgc atctctattg ctaataataa aatcaacagt gaattaaata agacgaagga
    12061 gcaattgatc ctcaaaggaa agcaaatgtt agctgatgag cttttgcgga tagaggaagc
    12121 aacctcatta aatggtcaaa atcagtttac aaatacacta tcggttgcta aagagagcta
    12181 cgaagcagaa gcatctaaaa ttgatgattt agtgatggaa aaatccaata tcattcgtaa
    12241 agattatgat atggcgaaac aggcttattt ggatgctgaa ctaaagaaac tagagcaacg
    12301 gtatgatgct gagcatttac cagaagttaa acaacaagaa gatgaatata gaaaagcaac
    12361 tgaagaaagt cttgatcaac agtaccaaga agcaattgaa tctattaaaa acgatgcatt
    12421 gaaacaaagc caagaagcaa aagaagttgt ggtggaacga attatcaatt cattgaaacc
    12481 agaaattgaa aaatcattaa cagacgtgca agaaaaaact ttagagattg tcgaaaaaac
    12541 aaagtcagaa aatgaagctg agttccatga tacaaaagag aaaacaacag atgtggttgt
    12601 caaatatttt gcgaacaaag aagaagttga aaagcaggta gctaacaaca cgaaagaatt
    12661 agaaacaaaa cttgtagagc aacaaaaaac tatcgaagaa tataaagatc gaatgaaaga
    12721 ggtcaaagat ttagcagagg gttataaatt aaatagcgaa aagttggcag aacaaagtga
    12781 agctttaaga gaagacttat ctcgttcccg aagtgatcta gctcaaatgg cagaaaaaac
    12841 gaaatcatta gcagttgcta cagaaaatca atcaagttca aaacgtgatt ttacaagtaa
    12901 aaagatggct actatcattt ctgtcgctgc aatggcttgc ggaacgatct tgggatcgtc
    12961 ctactttatt agtcataata atcaacaagc acaggctcaa actcaaacat ctatttcgag
    13021 agtggaaaat tcgattggga caaatacgaa ctctgcttca acaaatagta gctatgaagc
    13081 aacaccaact acagagttca aagttgggaa agatgttgtc atcagtattg atgggaaaac
    13141 aactaaggct gaagtgattt ctattgatgg agaaaaagca gttgtgaaaa ctccagacgg
    13201 aaatcagtac aaaattcaag ctcacttatc tgataataac taaggagggc tattatgcga
    13261 gaagaaaaaa tagtttatcc agtaattcaa aacaatcaag aaaacatgac ctcttctcag
    13321 cacttacagg aagcacagct cgtgtatcag gtaaatgaac taccacaaac gaacgagaca
    13381 atgagcaagt caatatcttt aattggcttg cttattgttg caatagtgat aactgtaggt
    13441 gtttaccgta agttgaacgt gaaaggaagt atttctgatg gcagataact caaactatgg
    13501 atttaaacat aacttacatc aaaaaccttc tgaaacaatt gtcagagttg aatggcttga
    13561 aaagcatgtc aatagcacct attctgttac agcaaaaaga ttaattgtat cagctatagt
    13621 tgttgcagtc gttgttcttg gatcaattgt ttacttagtt tctgatacga ttaaactgaa
    13681 atttattttt gttccaacgt tattaatgat aattggattt cttattctaa ttagccagta
    13741 tatgtttatt gatccatcta gtggttcatt gatttctggt tctattagac tgttcaaatc
    13801 agtagttaat tcagcaagag tatctatggg gaaaaagaaa ccatttcgag gcacagagat
    13861 tttaaaagtg aaacaagatg gaacaatcgt atatcgagat gggagtgtag cattaatgtt
    13921 caatctagat ggggctacat cactaaccgc ttttccagaa gaagttatgg ctcaggaatc
    13981 gaaatcaata caatatcaaa ataccagaga tcgttcctta gctgaaatca aaatcacttc
    14041 atctcaacct cagaatacgg aaagacaaac tcgaaatatg aaaatacaaa aacgaaatgc
    14101 aaggagtaac aaagcagtct ttgacataat agatcagcaa gaacgatttg tatcagaata
    14161 tattgatggt gtgaaaacaa cagtaatcca atatcttatc ttgaaatctc aatctaagat
    14221 ggtattggat gaaggtgtgg aacgtttaga aaactatacg agtagaggac tttattatag
    14281 tgtcactcag ttgtcagcta aacaaacaaa agaaatatta tctgatattt atgcactgaa
    14341 atagaggtga gagattaaat ggaaattatg ggtagatcac aactattaaa tttcaaaaaa
    14401 aagagaaaaa ctaaaaaaga acaaaaaact agaaattcaa aggtagtgaa taataccttt
    14461 gttagtgatt taacatcttt agttggttat caatttacac catcttacat taggacaggt
    14521 aagcggtggg gaacaattgt gaaattagtg aatacttatg gaatgaatag aaattatgga
    14581 tttggttgga tggttaaagc tattcctgaa gtgagagtag aaggggtaaa agcctatcta
    14641 attgaagcag ataagccagt gtcacaaaaa gatcaacaag aaataatgca cgagaatgtt
    14701 gggggatcaa cgaatagtta tgaaaataat ttagaaggaa aaaattcaga tgctgaaatt
    14761 agcaaaactg ttatgcggat tatggatttg aatacagcat ctttactaga cagcatggca
    14821 aataaaatta ttgattggaa gctagaaatc ctattagtat ccgattctcc taataaaatc
    14881 agcgaacagc taattaaact ccaacggttg tatgatgaaa aaatgtatgg attaaagctt
    14941 atgtcatatg gaggagatca acgtgatctg tttgatagac ttttaagtga accaatcggg
    15001 aatgtctatg agtatacgtc tatgagttcg gattttgcag gaaatgatca tgcaattcgc
    15061 aaaggtttag atgatgcgaa tggatgggct atcggcacta tgtctactag ctacgcaagt
    15121 ggtgtagctt ttatggcact ggatgattct tttacaagta aagttttagt ggcatcacac
    15181 aaaaacgcta caattaaagg ctatgatgaa aaaatctcag catcatcact ttgggggcaa
    15241 ttgattgcta ataatgcaat ggcaaatcat aaacgaactt ttcatattgt cttaaataaa
    15301 tttgattact ttggtgatcc aactggtaga acatttgttg ccaaaccttc gttaaacagt
    15361 gtaatagaaa aggttaattt ggctgaagaa ggtcttaact ctttggagct ttttggtaat
    15421 caaaaggaca ttggtgattt atatagacgg aatctcgata aactaactca gattttttat
    15481 ttgttagcag gcagaacatt agattctgca actagagttt tattgaaaaa agaattaggt
    15541 aacttttata taagtcagaa actttgggat attaattatg aagaacgttc aaaacttttg
    15601 gtgaacagta gtgatacaat gccagttctt ggggatttta ttagctattt gactaatgct
    15661 gaaacaaaag ccaaaaaatc tggtactgaa aaagaagtcg atcgtattat tgtattaaaa
    15721 gacacattga gaacagcatt agacagcttc tctaggatgt ttaatcaaag aacgacactg
    15781 aaagaaatcg gtaaaagtga caaattacag ttttactata acttaggtga tctaagagca
    15841 gaaaccaatg ttatggaagc tcaattttta aatacctttg atttcatcac atcacaagca
    15901 aaaaaagatg atattgttat gattcacggc ttcgataagt tatctattga aaccgttggg
    15961 ctaattaaaa aaagattaac aatgcttgaa gaagatggaa ttcgtcttgc ctttctattt
    16021 gatacaatcg gttcaggaga agacaatgaa tacaaagaag atattgaaac agctgatata
    16081 ttcaatacag atggaaaaat ttaccaaagt tttgaaagtg attttaatta cactatcatt
    16141 ggaaagatga ccagtggaga gttaatgaag tatgaaaaaa aagttgctcc tgctagaaaa
    16201 ggagaagatg atcaaaaact ttctgttgat ttgagacagt atttgacaac aggaagaagt
    16261 aataaatttc agatcaggag accagtggat ctgactaata attttgttat tggagagttt
    16321 attctttaag ttttatcttt gattttgagt gtataaggaa aggataagaa aaaacatgta
    16381 tatttgagac taaaaacagt cttagagaac atggtacgtt ttgtgaaaag agaactatag
    16441 aaatttcata aagtgaaacc ttactgagtg aaataattat agtggaggtt aaataatgaa
    16501 accaaacata aaaattttag atcgaatatt tctaggtaga gacacagaag taatcctaat
    16561 tcaacatgaa gaaggatttg aagttagtat aggtattcag aaacttcaaa aacctcatta
    16621 ttgtaatcag ttgtataaaa actttactga tgaagaaaaa gcgagagt
//
LOCUS       DAZKSR010000068        16078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000068 DAZKSR010000000
VERSION     DAZKSR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.068"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..335
                     /locus_tag="ACG8XG_002005"
     CDS             <1..335
                     /locus_tag="ACG8XG_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0773913.1"
                     /translation="STDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASV
                     SFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITF
                     MSYKKSKR"
     gene            complement(490..1476)
                     /locus_tag="ACG8XG_002006"
     CDS             complement(490..1476)
                     /locus_tag="ACG8XG_002006"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0773914.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1499..2065)
                     /locus_tag="ACG8XG_002007"
     CDS             complement(1499..2065)
                     /locus_tag="ACG8XG_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0773915.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2079..2495)
                     /locus_tag="ACG8XG_002008"
     CDS             complement(2079..2495)
                     /locus_tag="ACG8XG_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0773916.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2952..4886)
                     /locus_tag="ACG8XG_002009"
     CDS             complement(2952..4886)
                     /locus_tag="ACG8XG_002009"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0773917.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5188..6123
                     /gene="pplA"
                     /locus_tag="ACG8XG_002010"
     CDS             5188..6123
                     /gene="pplA"
                     /locus_tag="ACG8XG_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0773918.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6213..7316
                     /locus_tag="ACG8XG_002011"
     CDS             6213..7316
                     /locus_tag="ACG8XG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0773919.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7329..8282
                     /gene="menA"
                     /locus_tag="ACG8XG_002012"
     CDS             7329..8282
                     /gene="menA"
                     /locus_tag="ACG8XG_002012"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0773920.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9096..10442)
                     /gene="gor"
                     /locus_tag="ACG8XG_002013"
     CDS             complement(9096..10442)
                     /gene="gor"
                     /locus_tag="ACG8XG_002013"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0773921.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10576..11829)
                     /locus_tag="ACG8XG_002014"
     CDS             complement(10576..11829)
                     /locus_tag="ACG8XG_002014"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0773922.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11835..12758)
                     /locus_tag="ACG8XG_002015"
     CDS             complement(11835..12758)
                     /locus_tag="ACG8XG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0773923.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12979..15471)
                     /locus_tag="ACG8XG_002016"
     CDS             complement(12979..15471)
                     /locus_tag="ACG8XG_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0773924.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15478..15945)
                     /locus_tag="ACG8XG_002017"
     CDS             complement(15478..15945)
                     /locus_tag="ACG8XG_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0773925.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4718 a         3353 c         2859 g         5148 t
ORIGIN      
        1 atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc acaagtgata
       61 gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca agcgatacac
      121 gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac tttaacttaa
      181 ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct aaaactggaa
      241 gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc ggtttaagaa
      301 tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc
      361 aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac aagaactaca
      421 attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac gtatcgaacc
      481 tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga gattctcttt
      541 tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt ctgtgtaaga
      601 ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc
      661 gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta gctttggttt
      721 gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca tgtcttctag
      781 tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat cgataatctg
      841 aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt
      901 tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg tcttacctga
      961 aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat ccatctgccc
     1021 taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac ttttcaagga
     1081 agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc ctgcatagac
     1141 agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca gatccgcttc
     1201 atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag aagctgccaa
     1261 agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata gctgataggc
     1321 aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt cgacaggttt
     1381 gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt ctttacttaa
     1441 agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg tgtgacgatt
     1501 attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa tctttttgga
     1561 atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc
     1621 ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc catgattgtg
     1681 caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg gccgcactaa
     1741 ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt ccaaaataac
     1801 taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct agcaaagtag
     1861 tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg
     1921 caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat
     1981 aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt agcgaaatat
     2041 aaatcatttt ttgtaattta ctcatggtgt gtaaaggtct aataaatcag actgtcttcg
     2101 tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca gacgatggtt
     2161 tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca atctgcttct
     2221 tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg ttcatctttg
     2281 taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat ttctgtgcca
     2341 tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag accaaaaatc
     2401 atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg
     2461 agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt tccagattaa
     2521 ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat
     2581 aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct tattaccgag
     2641 aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg caacttcaag
     2701 aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc ggcttatttt
     2761 tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga ggttgtgaca
     2821 cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt ttacataaaa
     2881 aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt caaataacgt
     2941 tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca ccaattacct
     3001 agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg tccggaaccg
     3061 ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa catgcctgat
     3121 aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt gaataatcca
     3181 gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg gaagaacatt
     3241 gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc tggtgacttc
     3301 tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg tgcagcttga
     3361 gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc atcaccagca
     3421 gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg gaacacactg
     3481 tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac ttcaccattc
     3541 ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat acgaagtggc
     3601 acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct
     3661 ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga acgaatatca
     3721 aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc gcttaaatgg
     3781 atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat aaatccttga
     3841 tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat attcgctgcg
     3901 gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt tcctgggaat
     3961 tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta gccttttgcc
     4021 tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc aaagtcagca
     4081 gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt
     4141 ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt caataataat
     4201 tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg atccaacata
     4261 ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg atctaattta
     4321 taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc aataccggta
     4381 aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc atctggttcc
     4441 aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga atcttcaaat
     4501 gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt cggctctccg
     4561 cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt gacgattttt
     4621 cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt ttgacgagca
     4681 aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc agctacttca
     4741 tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat tgctacatct
     4801 tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata tcctgctcct
     4861 acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta tctatataat
     4921 gaatatatga taattctttt agtttgatac ttattacaca atctccatat attatataga
     4981 tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa taaagcgttt
     5041 tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt tatcaaaaat
     5101 agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa aaattgtgga
     5161 ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac tggatttgcc
     5221 gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa cggaagctca
     5281 aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac aacagaatca
     5341 tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt agaagaaaaa
     5401 aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg caaaatcact
     5461 gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac
     5521 aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc tgagttgaat
     5581 gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg agctactcac
     5641 tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca agctggtaac
     5701 acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta caagcttgaa
     5761 gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa
     5821 gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca agatgataca
     5881 gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta catccctact
     5941 ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc taatgtagaa
     6001 gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca acaattagtg
     6061 aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt aacagaaaaa
     6121 taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga ttaggcagtc
     6181 gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta ttttccttaa
     6241 tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca aataaaacaa
     6301 ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat gaacagttct
     6361 tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca gcattgaaac
     6421 ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa aaaggttctg
     6481 agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct gatgacgtct
     6541 acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga ttcgatatgg
     6601 cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga aaaccttccc
     6661 aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa ctaaatgaca
     6721 aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca
     6781 aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg accactgcca
     6841 tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc gaaaaccaag
     6901 actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta ggttctatta
     6961 aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta gaagtagatg
     7021 gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac aatgatatcg
     7081 caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct acagcagtct
     7141 tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat aacgggacgg
     7201 aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc aaagacacct
     7261 tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta aaataaggag
     7321 tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca aaggtggcta
     7381 gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat gagatacatt
     7441 ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg actaccgcta
     7501 tcaataatta catggattat gaaaaggctc attctgaagt atataagtat gaagaaaatg
     7561 tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta gctatgattg
     7621 catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt ttacttttga
     7681 tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt cctttatctc
     7741 gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt tttgccatgg
     7801 tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac ttttcatctg
     7861 gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta gcttccttac
     7921 ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat cttgaccgtg
     7981 atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg ggaacagtct
     8041 tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg ttgagcggta
     8101 tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag aagcatttgg
     8161 atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc aaaaatatgg
     8221 tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg caagccattt
     8281 aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa cagccaaata
     8341 aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
     8401 acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt
     8461 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
     8521 gaacaccgtt tatcagaggt tgtaaaatca gtgttttgtc ctgagtatta gagaaacaaa
     8581 tggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaagg acagctgatt
     8641 gaacactatt tgttcggaaa aagtgactaa ctatgacaca atttttttca cagtcccttt
     8701 tttcacttga tttgattcgt accagcgaac ttgacgagaa ctttcttggt ttggtaacta
     8761 tatggtattc ttttaacatc ctcgtttgct tcttctaatt tatttataca aagtcacact
     8821 tttttaaaca tctgttcttc gatacatttc tatttctttt aaacatcgat accaatattt
     8881 tgaacttaag agtgcatacc aaatggattc atttactctt cgtcagttac gaagataatc
     8941 aaaatctcca acttccctac ttatttcgtt ttgagaatta tcgaagcaat cttacataaa
     9001 taactaaaaa acaaaaaacc agaaaatcag tatactgatt ttctggtttt acatggtttg
     9061 ttcaataaaa agctgttgga actgttaata ttaaattatc gcatcgttac gaattcttcc
     9121 gctcctgttg gatggatggc tactgtattg tcaaagtctt ctttagttgc acccatcttg
     9181 atagccacgg caaaaccttg cagcatctca tcgacaccaa caccaatagc atgtaagccg
     9241 acgattcttt cttcttttcc gacacagatc aatttcattt cgcatttttg gcgataatca
     9301 tttaatgcaa agtacattgg ggtaaagcgt gaacgataaa ctttcaattc attttcgcca
     9361 tatttttcca atgcttcttc ttctgtcaaa ccaattgttg caattggtgg atgcgtaaag
     9421 acgactgtcg gaacaagttt atagtcaaga taagaatctt tctttccatt aaataaacgt
     9481 tctgataaac gtctaccagc tgcgattgca acaggagtca gatctaattt accgatcaca
     9541 tcaccaacag caaaaatatg ttcatccgtt gtattttggt atttatctac tttgataaac
     9601 ccttttttgt ccaattccac atttgtattt tccagaccta atttttcggt attcggtaca
     9661 cgtccaccgg caaaaagtac acaatcacca gttaaggttt caccattttc aaatagcacc
     9721 gtatattcgt cattgttttt agtaatttct ttagcaatat gatgtgcgta agtttttatt
     9781 ccagcttctt ggtacatttg gatcaaattg tccgaaagca ttttatcaaa cgttctaagt
     9841 ggtcgttctt ttcgatatgc ccacatgact tcactaccta aaccacgaaa tacacctgaa
     9901 agttcagccg caatgtatcc agcacctaaa acgattgctc tcttaggtaa ctctgttaat
     9961 tcaaaaaatc catttgaatc aattgcgtat tctccaccag gaatggtcat tttacttgca
    10021 cggcctccag tagcgatcaa gattttagga gcacgatagg tttcaccgtt tacttcgatc
    10081 gtttgactat cgataaaagt agcgtaacct tttatagcct cgacatgatt gctatctaat
    10141 cctctttgat aagcaccgtg tagaaaatca atatattttt ctcgtctttc tactagaccg
    10201 gcgaaatcaa cattttttaa atctgcttgg ataccgtaac ttgaggcatc tcgttcaatt
    10261 gtttccaaaa tcgtacttgc ttgccacata acttttttag gtacacaacc gacgttgaca
    10321 catgttccgc ccaattcatt cgcttcaatc agcaaaactt ttgcgccatg catccctgct
    10381 ctgtttgctg aagcaatacc gccagagccg ccaccaatta cgatgtaatc atatatttgc
    10441 attttgttcc tccttattga agagatcaat caaaaccttt atttaacgat cctttttcta
    10501 cagaaaacaa gtgtaacact tttcttgaat tttgtatgcg aagatgctta aaacagggat
    10561 atatcagctt tttccttatt tacgttttcg gatcatatct ccaggtttca ccggctgtgc
    10621 gactggcata gttaaaatca tcattggatt tggtgctcgg tcaatggatt cgccttcttc
    10681 attatgcata acttcaacgg tttgactgaa atgactgaat cctggaccat aaaattcgat
    10741 ttcatctcct acagaaaaca agtttctctg acgtatcgta gcaatctgtg ttgcctcatc
    10801 ataagataat acttcaccga caaatttata tactgggatt ttacgacgtt caccgaataa
    10861 ttgttcgttt tcagttggtg tattgtagta aaaacctgta gctaattctc gttgtgctac
    10921 tttccataac tcgtcgatcc attcttgctt gcagacatag ttttcaggat cttccatata
    10981 actgtctaca gccgctttat agacatttga caccgtagat acataatgga tcgatttcat
    11041 tcttccttcg atcttgaagc tgtctacgcc gttttcaatc aattctggaa tatgttcgat
    11101 cattgacata tccactgcac tcatcgagaa ttcttcttcc acttcgcctg tatcagttaa
    11161 actgtttcgt tcttgaccaa aaggcatatc gaacaatcca tacttccagc gacaagattg
    11221 agaacatccg ccacggtttg catcccgcat cgacatatgg ttggacaatg tgcatcgacc
    11281 agaataagag atacacatgg caccatggat aaatgcttcg atttcggtat ccgtattttt
    11341 acgaatctct gccacttcct ccattgaaac ttcacgagct aaaacgacac gttccaaccc
    11401 ttcttctttc cagaattcca atgtctcaaa attagttgct gaagcttgag tggataaatg
    11461 gatcggcaga cctggggctt ctgtcgcaca aatttcgatc aaagctggat cagaaacaat
    11521 cactgcagaa ataccgacat cacgcaaact gcggaaaaat tcccctgctc catcttcatt
    11581 tccttcatgg gtgaccatat tagcagcaac gtataccttc gcatcatgtt cttttgcaaa
    11641 agcaactcct tcagccattt cttcataagt aaaattacct gcacggcttc ttaaaccata
    11701 tgcattcccg ccaatatata ctgcatctgc tccataatgg atcgcggttt ttaacttttc
    11761 cagtgttcct gctggcgcta ggatttctgg tcgtttcaat tttcttttta ctgtcatttc
    11821 tttcatcata ctccttattt gatagcagaa ggatcaatat aaaagaatcc tgtatctaat
    11881 tcacggtttt caggatgcaa tttactgatt tcgctagtca ttttttccgc tttttcttga
    11941 tcccagctac cttctaaaat ttgttttttt gcttcggcga atagtcgagt gatgtttacg
    12001 aatttttcgc ctggagtaaa taatccgtcc aatttccatg tacagtaatg atgctgataa
    12061 agttcattca attcattcga gagattcaga tcattgctgg caaaaatatg tgtgccatga
    12121 ctatcttcat aaatagaata atgtgtctct tcttttttag gttccgagat aaaaagacct
    12181 ctttcacgat ctttttgttc gtcttgtttc gtatagttat agtaattctg tagcagtgga
    12241 cgtttggatt gatggatgca tgtagcacca tacacaaggg tttctacagg aatatcgagt
    12301 ttttcttcca tcgccttcat ctcttcaaaa ggaacttcac gagcaagtac tgctccaact
    12361 gcgccttttt tcgcccagaa attgatttga cgtgcgctcg ttactaatgt ttcagcatca
    12421 taaatatacg gtatacgcaa ttcaggattt tttttcatcg tataaataat acctgtatca
    12481 ccaactgtga tcacatctac gtttatttct tttaaaaaag ccaaatattc aggtacattt
    12541 ttcatttttt caggatgaat gatcccattt acagcaatat tcgctttttt accatacttg
    12601 tgtgctaatt ctaccaattg tcgctgttct tctcgtgtaa atgaagcagg cagacgcaat
    12661 ccaaattgct cttcaccgaa atatagtgta tctgcacctg cagcaagcaa ctgttctgct
    12721 tgttcaatag tttctactgt ggttatgagt tcgatcataa ttttctcctc ctacaacttc
    12781 ataatagtag catagctcta aaaactttcc aatacttctt ctatttaaag tcggattttt
    12841 gaaaattatt tcacaaaaaa tcaaaataaa gttcgatata ttctgtccaa atataaaaag
    12901 agattgtgac aaaaatcttg tcacaacctc ttaatcaagt cttactatta taaaaattca
    12961 gctacacagg ccaagtaact atagggaacc tactgtttcc tttttaggtc cgcgaacatt
    13021 caatgtgatc ttaccttttg aagcacctac tgttacgtgg tcacctaaac gaatttctcc
    13081 gccaagcaaa gcctcgctca atcgatcttc aatttctttt tgaagtgcgc gacggattgg
    13141 acgtgcacca tattcaggat caaatccagc tttcccgatc acatcgattg cagatggtgt
    13201 gattttcact tgaatatctt gttctttcaa tcgtttgata atcgctttgc tcatgatttg
    13261 gacgattgta tggatttcat ctttactcaa tgaatggaag acgacagtct catcgattcg
    13321 attcaagaat tcaggtcgga aagctttctt taactcttct aagatacgtt tttgcatggc
    13381 tttgtgatct ttagtaacat cttggacatt gaatccgaca tttttttctt ctcgaatttg
    13441 tgtcgctccg atatttgaag tcataatcat gattgtattt ctaaagtcca cttttctacc
    13501 tttagaatct gttaaatgac catcatccaa tacttgcaga agtaaattga aaacgtctgg
    13561 atgtgctttc tccacttcat ctaagagaat cactgaatat ggtttttgac gaattttttc
    13621 tgtcaactgt ccaccttcat cataccctac gtatcctgga ggagacccga ttaatcggct
    13681 tgtactgtat ttttccataa attctgacat atcaacacgg atcagggcat cttcacttcc
    13741 aaacatgact tctgaaagtg ctttggccaa ttctgttttt ccgacaccag ttggtccaag
    13801 gaacatgaat gaaccaattg gacgatcagg atctttcaat ccgcttcgtg cacggcggat
    13861 cgcacgtgaa actgctttga cagcctcatc ttgaccaacg acacgttcat gcagaagtcc
    13921 ttccagttct aacaagcgtt cgctttcttt tttctccaac tgctgtagag ggacaccagt
    13981 ccactccgat acaactgtag caacatcttc ttctgttaca cgatcagaat aaccttgctc
    14041 ctgttttgct tcaattgcag aaactttttg cagcttttta cgtagtgctt tttcttgtct
    14101 gcgtaattga gcagcatttt caaagtcttg tttttggatc gctgcttcct tttcttgtac
    14161 tagttcatcg atttctagtt tgatcacagt tgattttgtt aagtgatcgg tttgatccaa
    14221 acggactttc gctgctgatt catcgatcaa atcaattgct ttatcgggta gttgacgaga
    14281 agtgatataa cgaacagaaa gattgactgc tgctcgtaca gcttcatctg tgatctccac
    14341 tccatggtgc tcttcatatc ttgtacgtaa tccttgaaga atcacttctg cttcttcagg
    14401 agttggctca tctacttgaa tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat
    14461 atatttttga tattcatcta gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa
    14521 cgccggtttc aagatattcg aagcatcaat tgctccttct gctccacctg caccaattaa
    14581 ggtatgcaat tcatcaatga ataagattac ttgaccatca ttatagattt cgtcgatgac
    14641 ctttttcatt cgatcttcaa attcaccgcg atatttcgta ccagcaacca aagatcccat
    14701 atcaagcatc atcaggcgct tttcttgcat gtcttctggc acttcaccta aaatgatttt
    14761 ttgagctaac ccttcagcaa tggctgtttt gcctacacca ggttcaccca ccagtacagg
    14821 gttattcttt gtccgtcggc taagaatctg gataagccgt ttcacttctc tcgaacgacc
    14881 aacaactggg tccagcttgt tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc
    14941 tagcgtaggt gttccttgcg gtgtttgccg tttatcttgt acatttacat ttctacgacg
    15001 attcattcct tttccagcag aatcaccagc tagccccatt ttttgtttca gaacttgacg
    15061 ggcttttgaa aggctgatac ctaagttgat catgattcga gtagccatga tactttcatc
    15121 ttttaataag ctcatcaaaa tatgttctgt tccaactttt tgagaaccag ttttcttcac
    15181 ttcgctgcta gctaaagcaa aaacttgttt cattcttggt gaatatggaa gaaagactcc
    15241 tttagggtaa gaacggatac caccataacc aatcagatgt tcaatttctt cataaatttc
    15301 cgtttcattc aaatccattt cacgtaatac ttttccagca attccatttt gctcgaccac
    15361 taaggctagt aaaacatgtt ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc
    15421 ttgtgcaatt tctaatactg cttgggctct ttcagtaaaa agttcttcca tctaatctca
    15481 cttcccatct tcataactta aacgttccag aaatgctcgg agaatccgag cacgtgtatg
    15541 atcttcatat tcattgaaat tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc
    15601 ttcttttttt gtaattattt ggtcttcata aagtttttga ataatagcaa aagcattttt
    15661 ctcactgatt gtatcatcaa ataactgatt tatctgctca agtaattgtt ttttatctga
    15721 aatccttact tttgcaattc gtatataacc accaccacca cgtttgcttt ctactagata
    15781 accacgttgg atggtaaaac gggtattaat cacatagttg atttgtgaag gaacacagtt
    15841 aaagaggtct gccatttcaa tccttcgaat ctcgatcatt tcactatttt ccagaatttt
    15901 ctttatatat gattcaatta aatccgaggt attctgatgt gccatcttat tcattccctt
    15961 tgactaatat tgaccttaat tataacgaat ttgataatag aaagaaagaa gaacgctttt
    16021 tgttaaataa atcctttaga atcaacaaaa aaaaatacgg aatatactat tccgtatc
//
LOCUS       DAZKSR010000069        15229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000069 DAZKSR010000000
VERSION     DAZKSR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.069"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8XG_002018"
     CDS             278..787
                     /locus_tag="ACG8XG_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0773926.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8XG_002019"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8XG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0773927.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8XG_002020"
     CDS             2322..3302
                     /locus_tag="ACG8XG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0773928.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8XG_002021"
     CDS             3424..4842
                     /locus_tag="ACG8XG_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0773929.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8XG_002022"
     CDS             4913..6382
                     /locus_tag="ACG8XG_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0773930.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8XG_002023"
     CDS             6392..6859
                     /locus_tag="ACG8XG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0773931.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8XG_002024"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8XG_002024"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0773932.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ACG8XG_002025"
     CDS             7848..10382
                     /locus_tag="ACG8XG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0773933.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ACG8XG_002026"
     CDS             10538..11311
                     /locus_tag="ACG8XG_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0773934.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ACG8XG_002027"
     CDS             11304..12095
                     /locus_tag="ACG8XG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0773935.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ACG8XG_002028"
     CDS             12105..12596
                     /locus_tag="ACG8XG_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0773936.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ACG8XG_002029"
     CDS             12614..13024
                     /locus_tag="ACG8XG_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0773937.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ACG8XG_002030"
     CDS             13028..14428
                     /locus_tag="ACG8XG_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0773938.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ACG8XG_002031"
     CDS             14451..14825
                     /locus_tag="ACG8XG_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0773939.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DAZKSR010000070        15026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000070 DAZKSR010000000
VERSION     DAZKSR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.070"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(510..1076)
                     /locus_tag="ACG8XG_002032"
     CDS             complement(510..1076)
                     /locus_tag="ACG8XG_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0773940.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="ACG8XG_002033"
     CDS             complement(1076..2032)
                     /locus_tag="ACG8XG_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0773941.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="ACG8XG_002034"
     CDS             complement(2032..2688)
                     /locus_tag="ACG8XG_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0773942.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="ACG8XG_002035"
     CDS             3058..5076
                     /locus_tag="ACG8XG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0773943.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /gene="leuS"
                     /locus_tag="ACG8XG_002036"
     CDS             5503..7917
                     /gene="leuS"
                     /locus_tag="ACG8XG_002036"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0773944.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="ACG8XG_002037"
     CDS             8265..9818
                     /locus_tag="ACG8XG_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0773945.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="ACG8XG_002038"
     CDS             complement(10199..11983)
                     /locus_tag="ACG8XG_002038"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773946.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="ACG8XG_002039"
     CDS             complement(11998..13719)
                     /locus_tag="ACG8XG_002039"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0773947.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="ACG8XG_002040"
     CDS             13819..14580
                     /locus_tag="ACG8XG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0773948.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4895 a         2755 c         2967 g         4409 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaactcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 acattattac aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt
    15001 ttaatcaaat agtccctact gtgact
//
LOCUS       DAZKSR010000071        14993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000071 DAZKSR010000000
VERSION     DAZKSR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.071"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACG8XG_002041"
     CDS             <1..607
                     /locus_tag="ACG8XG_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773949.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            627..1655
                     /locus_tag="ACG8XG_002042"
     CDS             627..1655
                     /locus_tag="ACG8XG_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0773950.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1652..2410
                     /locus_tag="ACG8XG_002043"
     CDS             1652..2410
                     /locus_tag="ACG8XG_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0773951.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2496..3236)
                     /locus_tag="ACG8XG_002044"
     CDS             complement(2496..3236)
                     /locus_tag="ACG8XG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0773952.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3297..4550
                     /locus_tag="ACG8XG_002045"
     CDS             3297..4550
                     /locus_tag="ACG8XG_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0773953.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4845..5099
                     /locus_tag="ACG8XG_002046"
                     /pseudo
     CDS             4845..5099
                     /locus_tag="ACG8XG_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5221..5547
                     /locus_tag="ACG8XG_002047"
     CDS             5221..5547
                     /locus_tag="ACG8XG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0773954.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5615..6328
                     /locus_tag="ACG8XG_002048"
     CDS             5615..6328
                     /locus_tag="ACG8XG_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0773955.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6638..7490
                     /gene="licT"
                     /locus_tag="ACG8XG_002049"
                     /pseudo
     CDS             6638..7490
                     /gene="licT"
                     /locus_tag="ACG8XG_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7611..9476
                     /locus_tag="ACG8XG_002050"
     CDS             7611..9476
                     /locus_tag="ACG8XG_002050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0773956.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9490..10933
                     /locus_tag="ACG8XG_002051"
                     /pseudo
     CDS             9490..10933
                     /locus_tag="ACG8XG_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11041..11742
                     /locus_tag="ACG8XG_002052"
     CDS             11041..11742
                     /locus_tag="ACG8XG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0773957.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11868..12380
                     /locus_tag="ACG8XG_002053"
                     /pseudo
     CDS             11868..12380
                     /locus_tag="ACG8XG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12778..12987)
                     /locus_tag="ACG8XG_002054"
     CDS             complement(12778..12987)
                     /locus_tag="ACG8XG_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0773958.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13228..13803
                     /locus_tag="ACG8XG_002055"
     CDS             13228..13803
                     /locus_tag="ACG8XG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0773959.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13803..14243
                     /locus_tag="ACG8XG_002056"
     CDS             13803..14243
                     /locus_tag="ACG8XG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0773960.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14259..14960
                     /locus_tag="ACG8XG_002057"
     CDS             14259..14960
                     /locus_tag="ACG8XG_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0773961.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2484 c         3161 g         4282 t
ORIGIN      
        1 agaaaatcaa ctttttgcag gagatgtatt agaaattgta ttaaacgacc aagcaggaga
       61 agctgctgct aagggtgtgc agaacaagcc aagtaccaac gatgaaattg ggaatcacaa
      121 tccaagtgat acgccagttg tttatcatga tgccccgcct tttgcacctg ctacgaagat
      181 tgtagtagaa ggtggtctct catttcttgc cccttccaaa ctcgattgtg gtcagatcaa
      241 agcaactggg ttgacgcaag aagcatttgg ccaaatggat atacaggaaa aattatggat
      301 ttatgatcag cgagaaacaa aaagtccatg gatcttaacg ataaaattat ctcagccttt
      361 cacaaccgat gcaggatttt ctatgcagga tgtcctatat ttcaagaaag ctgaggagta
      421 tcaattagtg aatgaagaag aactggaaat tgtcagagga caaaattcaa cgaatgtgca
      481 aacagattat tcagaatatt taaataatgg tcgggcgttt aaacttgtct taaccaaaga
      541 aaatcagata gctggtaatt actcagcaga gatgacatgg acattagcag atgcgccgac
      601 agaataaaga tcaaggagaa tgattgatga atcgaatatg gaataaaagt catcgaaaca
      661 actgctcttt acttctgatc cttctccttg gattcatcct ttggcagaca cctgtccatg
      721 cctccgaatt caattttgcg gtagattttg aattaccggc taatcagatt gataagaaca
      781 aaacatatat aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc tacacggtct
      841 ttaaccgaac agataaatca gtaacagttg agacattgat ccactccgct acgacgaatt
      901 caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca ttaagttatg
      961 atatgaaaaa cttagcaaaa acagtgggag aaataactgt ccctcctcaa agtgcggtca
     1021 ctgagacact ttcactgaaa gctccagaaa aagagctggc tggtgtggtc gcaggaggaa
     1081 tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt gtcgtcaatc
     1141 agttttctca agtgaaagca atcttgattc gaaatagcga aaaagagtct tccccaaatt
     1201 tgaaattaaa aaaaacttat gcggatcaaa gcgatggaag aaatgtgatt aaggcgaatc
     1261 ttcaaaatcc aaatgcttct tatatgttcg gcttgactac atcggttgaa atctacaaaa
     1321 aagaaacaaa agagccttat ctttcaataa aaaaagaaaa aatagatgta gcacccaata
     1381 caaattttga tttacttgtc ccattagaag gaaaaaaatt agctccggga cattatcaag
     1441 cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct ccataccgat tttgaaataa
     1501 ctgatgatgc agcggaccaa ttgaatgaaa aggatattga tttaaaaaat cagccagaaa
     1561 catttgactg gcgatggctg atacttgttg gagtcattct tattggctta tttgctggat
     1621 tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa acaattgcat taagtaccgt
     1681 tctagctgga agtctattat tatgtacaca aagtgttttt gctgagaata agagctcagc
     1741 cactacagaa ggaacgattt ctgtaacaaa accagatgcc gcaaatcctg aagacgttgt
     1801 tccagttcct cctgttgatc caaatgatcc cgatacgccg tttacaccta acgaaccaaa
     1861 tcagggaaca tccggattac tgacgattga ccaagctcca gatcttgatt ttggaacaat
     1921 caagttaggt acagcaaaaa cagtatatgc tgcattacaa aacggaacgg acagtacagg
     1981 gacagcaaca caagtacaga atttcgtaca agtaacggat aaaagcggaa tttatgccgg
     2041 ttggaaacta tcagtcaaac gaacagaatt tattggtaca acatctgata cgaataaact
     2101 aactggttca aaggtgaaat tcaaaaacgc cgtgattcga aatagcgcaa ctaatagcag
     2161 tggaacgcca tctactattt ctggtactgg cgctgaagga attgagatcc cagcagatca
     2221 ggcagtagat ctagtaactg caggagcaaa tgaagggatt ggcacatgga tttattcatt
     2281 aggagagaat gtggaacaag ggagacaatc agtggaatta tttgttcctg tatccaatta
     2341 tgccgtagac aactacgttt caacattcac atggtcgtta acagatgctc ctacagaagg
     2401 gaaagcgtaa aatcaaatca atgacttcag ggagtggagc cgattcaagg ccaactccct
     2461 gaaatctgct atgcttacgt ctcagctctt attcatcaac tcgaagcagt tttttggaag
     2521 tcatggaaac gttcctttgt cttttcaaag atctttcgac caacgagtaa ggagtcacca
     2581 ttgaaaaatt ctaacacata atgagcagaa tcaattttct gcaattgcga taagttgata
     2641 cagtaagatt ttaatagtaa aaagtaagca tacttttcca actctgtctt caattgtttt
     2701 aaagaagtat tcactaattt atcttctgta gttgttttga ccataacttg gtttttcaaa
     2761 cctttgatac tttcaatata taaaatttcc cgaggataca ggacgagact tccacctttt
     2821 tgatcaataa agattttatc gacatttacg atttgctgtt ctttccaatt gatctgttct
     2881 agcacttgac gaatttgttc agataaattc ctgtcttttc tgatatatcc aatcggaagg
     2941 attttccgat tgatgatctc tactgcaaaa ttcgtataag cggtgaaaaa aacaatatta
     3001 ctatgtgcat tttttttacg tatttgttcg gcgacatcaa tacctgtgag tgattgcttc
     3061 aagtcgatat cgataaagaa aacatcgtga ttatcaatag tacccaactg gatcaactga
     3121 ttgatttgag agatagaagt gagctgcacg atattctcga tatgatgatt gaccaattct
     3181 ttgatgatcc attcgctgtg aaactggtta tcttctatga taaaatactt tttcatatgc
     3241 aggctcctat taaaaaaata tgtgtacatt ttgaaacaaa acaatggagg aataagatgg
     3301 tcgcaacagt gacactttat ttgaccttta ctttgatttt agatttaggt atctatttat
     3361 tcttttcgaa acaactgcta gtaaaaaaca gctttggttt tatagctttg atggtggtga
     3421 cacttttatt tcctatccct aaagtgagct ttccttacct agttggctta ttattgatgt
     3481 ttctttttta ttttactacg aaaaacctgt ttatcagctg tttgatcagt aatctttttt
     3541 ttgctatcac tggactcagc ttttttctgg tttttgatct gatggattat ttaggaaaga
     3601 gacatcttgg aacagcaagt ttttttgcac tacttatcgc atttattttc tatctattag
     3661 taaaaagtat agacaaacag acgagatttc tcaacttgct ttttacttat ccaactgaac
     3721 actattttgg aggtatttgt attttattaa gctggggcgt gatttacttg gtattgggaa
     3781 gtgttcactt ccgctcatct aattatctgc ttctatctct tttgtcgata gtcatcagtt
     3841 ttttttcttt gtttttttcc attatgttga tcagtttcca agtaaaagaa agacaacatc
     3901 aggaatcgct tcaactgtat caaataaatg aagaatatta ccatcatcta gaggaattca
     3961 aacatgatta taaaagtcta ctgttttcat taaaaattac tcttcaggaa aataaaaaag
     4021 aaacgttgga atttttagaa atcttagaag atcattctgc agctattttt gaaaaaccgc
     4081 aaatacaaca gctaacaaat attgtatccc cagctgtcag gggtgtcttt ttaaaatttc
     4141 tagaaaaagc agaatcggag aaaatcccga tccaagtagt tattcctgtc gaagtgaaaa
     4201 caatcccgat tgatctggtt gtttttattc gctgtttatc tatttttatt tctaacgcat
     4261 tcgatcatcg tgtagccgat cagagtccga ttacgatcaa tctgctggaa gaaaaagagg
     4321 gattacgttt tagtatagcg aacaaggcaa atccgacgaa tttgacgcta tcggagatgg
     4381 tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg tctatacagt gcgaaaaaaa
     4441 tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag aatcactatt
     4501 tttttgtaga aatgtatctg gcatctagaa agagcaaatc agcctactga cttactgtca
     4561 aaggataagc ttgaggaaag tctacacgga gatagagaca acaacacagc atattcaaaa
     4621 gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt agttaaacaa
     4681 agtagttgac acttccgcta cttagtatta gtacgaaagt atacttcatg atactaagta
     4741 gataagaagt gaaaagcgga acccacgatc gtcaaaatgc taactgcttt gcttacacca
     4801 gataaaggga agagcaatat cgcttatttt aggcgtggag aagcaagaaa atccctacga
     4861 tcacacaaaa aatgctgacc cagcagttaa gagagttaga acaagatggc attgtccatc
     4921 gaaaagtcta taaccaagtc ccgcctaaag tagagtactc gctcaccgaa gaaggaaaat
     4981 cattaagaga gattctagta gcgatgtctg tttggggaga agaacgtatc gaaaaacaaa
     5041 aagcctcagg ggaagaagtc acattactgc atacacatgg tggctaccta aattactgat
     5101 ttctatgaca tttattttaa ataaaagaaa acaggagttt ttttaaaagc atcatctatt
     5161 ttttatgtaa aaaaatggta tgattaatca agtaagaaat gattggaaga aggcaagcaa
     5221 atgaatagca tggaaaaagt taaccagttt agagatgaac gtaattggcg acaatttcat
     5281 aacgaaaaag atttggctat ttctatttct ttggaagcat ctgaattgtt agaacttttt
     5341 caatggaaaa cgccggaaga agtaaaagaa acacaattag aacgaatcaa agaagaactg
     5401 gcggatgtat tgatctattc atacatgatg gcagacaacc ttcagctgga tctagatgag
     5461 atcattgaag aaaaattgat caaaaataat ttgaaatatc ctgtagaaaa aagcgcaggt
     5521 gtacgaaaaa aatacacaga gttttagaaa tgtgtcattc ggctgttgga aagagcaaaa
     5581 gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa aaaaaaggaa aagcaaaaag
     5641 atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt tgattttcaa
     5701 tgaacagatc aaggattatc ttgtcagaga gacaggagat aaatatgcta tagcgaatgt
     5761 aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact ttgatgcggt
     5821 agaaccaatg acgacagaag gggtcatgag gtcgcagatg agaggaacag atctaccagt
     5881 gattgctagt atcgcagtcc cttcagtttc aatcaattta ccgattttca aaggcttgga
     5941 taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa tgggaaaagg
     6001 aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgttgttta caccgctgga
     6061 aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt atacatacaa
     6121 aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg aagtcgagga
     6181 aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc gactagttgt
     6241 acaaggagaa ctggaaagtg ttacaccgat aaaagatgcg acagatgata tgagatctgc
     6301 tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta cagattaaaa
     6361 acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa gaggaacgca
     6421 aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag tttttttatt
     6481 ttttatatga aaaggcttgc ataaataaaa attcgatggt atgatgattt tgaataagaa
     6541 atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag taggcgaatt
     6601 ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata aaattttgaa
     6661 taacaatgtc gtcatttcga aaaatggatt cggggaagaa gtcgtttgta tgggaaaagg
     6721 actagctttt caaaaaaaag attggggacg agatttcgcc agaagcggtc caaaaagaat
     6781 ttattttaag agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg
     6841 cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg
     6901 aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag
     6961 caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga
     7021 aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac
     7081 tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg
     7141 aagacatgca agtgacgatg accgctacca caatggtaag agatatactg agtatcatca
     7201 gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc
     7261 atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat
     7321 ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca
     7381 atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa
     7441 cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac
     7501 tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt
     7561 ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc
     7621 aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag
     7681 tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa
     7741 agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag
     7801 tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag
     7861 tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct
     7921 ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct
     7981 tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt
     8041 ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta
     8101 aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag
     8161 ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc
     8221 tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa
     8281 gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc
     8341 cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga
     8401 cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg
     8461 tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg
     8521 gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa
     8581 tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa
     8641 aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca
     8701 ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta
     8761 tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca
     8821 gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa
     8881 atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat
     8941 tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg
     9001 caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac
     9061 tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg
     9121 ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt
     9181 ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata
     9241 tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg
     9301 atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg
     9361 gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg
     9421 aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag
     9481 gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc
     9541 aaatcaatta gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat
     9601 gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga
     9661 tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga
     9721 agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac
     9781 acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga
     9841 tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta
     9901 cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga
     9961 cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg
    10021 gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt
    10081 aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt
    10141 tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg
    10201 catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac
    10261 aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata
    10321 ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca
    10381 aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta
    10441 ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt
    10501 gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc
    10561 agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat
    10621 tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga
    10681 ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg
    10741 tgtagatttg atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg
    10801 agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac
    10861 actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg
    10921 cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt
    10981 gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt
    11041 atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc
    11101 tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt
    11161 caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc
    11221 gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc
    11281 aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg
    11341 ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag
    11401 caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt
    11461 cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg
    11521 tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca
    11581 gtatttttaa cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa
    11641 cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac
    11701 catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg
    11761 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    11821 tcgtttggtt aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg
    11881 gataaggggg tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt
    11941 tagcttcgat ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc
    12001 gtaaaaatat actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga
    12061 tctgtcagat cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca
    12121 tagcaacggg agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata
    12181 gccgtattcc ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca
    12241 ttggtgcatt gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac
    12301 cagtagatcc gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg
    12361 atgatttgac aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg
    12421 taccttgaga caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt
    12481 ttggtttagg agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga
    12541 aggaaaaatg agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag
    12601 gaaacaaaaa aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa
    12661 aaagaaatta ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag
    12721 ttccaggcag gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca
    12781 agtgatattt ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg
    12841 atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac
    12901 atcatttatc agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taaaagaaac
    12961 aagtatttgt tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct
    13021 tctataagta agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata
    13081 aaatggctga aaaatgattt ttttctaaat actatatata gtggcgtcta tcctatatac
    13141 cactaaaaaa ctacaaaatc tagtctttta ttttaaaaaa acacgtgtag aatgaagata
    13201 gacttttgag ttagggagtg tttttttatg aaacttactg ttttttgcgg ttcccgtttt
    13261 ggtaataaag aaagttacaa atttattgcg caaacgttag gaaaatacat ggcacaagaa
    13321 gaaatcgaac tggtttacgg tggttcatac tccgggatta tgggagtgat tagtaaaaca
    13381 gtactggaaa ataacggaaa agtcacaggt atctatccga atgggctttt tgaaactgaa
    13441 ttgcctggaa aagatgttac tacatttatt cctacagaaa cgatcgatga aagaaaggta
    13501 ttattatttg aaaagggaga tgctgtcctc gttttccctg gaggtctagg tacattggag
    13561 gaattctcac aacttctttc ttggatagca atcggtctga cgcccgacaa gccaatcgga
    13621 atattgaata ttggcggtta ttacagcggc ttgcaacaat tattagaaac ttttgcaaaa
    13681 gaggatttta tggataaaaa atggttggat caagtaatct tttccaacaa tccattggaa
    13741 ctggtcaatg aattacgagc agtatctatt aaaaatcagt tattattgaa ggaggcaaaa
    13801 taatgagtca aatatattta gcaggaccat ttttttcaga ggagcaaatc gatcgagtaa
    13861 gtcgtatcga aaaagcgttg gaggaaaaca aaacagtcac aagtttctac tctccacgtc
    13921 atcaccaaga aagcaattat gaactattca gcgctggatg ggcgcaggaa gtatacgaaa
    13981 aagatatgga agaactgaca aatgcagaat ttgttgttgc tatccttgac tttgagcatc
    14041 aaacaatcga tccaggaacg gcttacgaat taggtgtggc aactatgttg aaaaaaccga
    14101 tgatcattgt acaagaagag acggtaccta cgaacttgat gattacacaa agtctccata
    14161 cgtatttaaa atcagaccaa gctgtacgtg aatatgattt tgaaacattg cctgttgaaa
    14221 cttacgtagg cgagtactta tagaagagga agggttgtat gtcagtgatt ttgatttcag
    14281 gaacgattgg tgcaggtaag agcagtttga cggatatgct tgcaaaagag attgattcta
    14341 aaccatttta tgaaaatgtc gaagataacg aagtattgcc gctcttttat tcaaatcctg
    14401 agcagtacgc ttttttattg caaatcttct ttttgaacaa gcgattttta gcaatgaaaa
    14461 atgctttagt gaacgatgac aatgtgctag accgatccat ctatgaagat agcctgttgt
    14521 tccatctaaa cgctgattta ggtcgggtaa cggatatcga agtacagcag tacgatagct
    14581 tattagatac gatgctaaac gagttagatg atgttgctcc aaaaaaacgg cctgatttga
    14641 tggtccatat caaagtttct ttggacacga tgctagaacg tatcaaaaaa cgtgggcgtg
    14701 actatgaaca gctagaaagc gatgagacgt tatataccta ttatgaaacg ttgaacaccc
    14761 gctataatca atggtatgaa gattttgatg tatgtcctaa aatacaagtg gatggggata
    14821 aattcgattt tgtagaaaat ccagaagatg caaaaaaggt gatccgtcag atccaagaaa
    14881 aattagctga attggaagga aagcctgctg gaaaatattt tacggataac cagcatgcaa
    14941 aaggtcaaga attaggctaa aaataaaaaa ccaaacgacg ggaaagaaaa att
//
LOCUS       DAZKSR010000072        14862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000072 DAZKSR010000000
VERSION     DAZKSR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.072"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(343..1071)
                     /locus_tag="ACG8XG_002058"
     CDS             complement(343..1071)
                     /locus_tag="ACG8XG_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773962.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1049..2623)
                     /locus_tag="ACG8XG_002059"
     CDS             complement(1049..2623)
                     /locus_tag="ACG8XG_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0773963.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2852..3790
                     /locus_tag="ACG8XG_002060"
     CDS             2852..3790
                     /locus_tag="ACG8XG_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0773964.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3802..4713
                     /locus_tag="ACG8XG_002061"
     CDS             3802..4713
                     /locus_tag="ACG8XG_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0773965.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4737..6209
                     /locus_tag="ACG8XG_002062"
     CDS             4737..6209
                     /locus_tag="ACG8XG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0773966.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6260..6871
                     /locus_tag="ACG8XG_002063"
     CDS             6260..6871
                     /locus_tag="ACG8XG_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0773967.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6901..8814
                     /locus_tag="ACG8XG_002064"
     CDS             6901..8814
                     /locus_tag="ACG8XG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFQ0773968.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8942..11923
                     /locus_tag="ACG8XG_002065"
     CDS             8942..11923
                     /locus_tag="ACG8XG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFQ0773969.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11920..13581
                     /locus_tag="ACG8XG_002066"
     CDS             11920..13581
                     /locus_tag="ACG8XG_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFQ0773970.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13621..14553
                     /locus_tag="ACG8XG_002067"
     CDS             13621..14553
                     /locus_tag="ACG8XG_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0773971.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4829 a         2342 c         2985 g         4706 t
ORIGIN      
        1 agttttattc aaatttgtct tcaccaacac gatttgacga agagcctatt taaataacta
       61 catccattta ttctatcttc taaaatagcc tttttcattt cagttaaata gttcagcgcc
      121 atttttgcac tcgacttgca tagtagtttt ttccatattc ctcaacctca gcgggggttg
      181 aagacataag gaaaatccta tgcgttatgc tcaactggat ctgattaaat attctaatgt
      241 atttttctct accatcatta aatggttaat agccaaaaat caccactttt caatggctcc
      301 ttgttgtata agtataacta gaagatgaac acgtctgaga aattatagaa gctttttacg
      361 gtaattactg gggctgtgtc cagtagtttt tttgaagaga acagaaaaat agctcacgtg
      421 atctattcct actttttgac taatttcatc aatagataag aatgtgtctt tcagtagagc
      481 acttgctctt ttgattcgaa agtctagcaa gtaatcatga aaggaataac ctgtttcttt
      541 tttgaaggcc cgtcctaaat aagcgggatg gtaatggaat tgtgtagcta catctttgag
      601 tgtaagcttt tcactataat tgttttgaac gtaatcaatc acttgttgaa ttgactcatt
      661 aaaagaatca tcagttagag agttttgatt agctgctgat ttacgcattt tttcttcttg
      721 tactaactta actaaataag gaaaatcaat gggtttcgtt atgtaacttt taatagataa
      781 atcaatcccc ttttttgcat actgaaaatt ttggtgggca ctaatgacaa tactagtaaa
      841 gtagcgattg gatgtttgtt gcaaagattc gattaattcg agtccagaaa ttttccccat
      901 cgtaatatct gtgataagta aatcaaagcg ctggttttgc aggtttttaa atgcttcgtc
      961 tccgtctgtg tagcagtgat cgataatgat gtcggggact ttcttcaaaa gataatgcct
     1021 tatgccttct acaattgtac aatcatcgtc aacaattgca attctcaaca tgcatattcc
     1081 cccctttttg tattttgata gtaatcgttg ttccgacgtg tagttggctt tctataacaa
     1141 aacgtgtatc tccaggatag agatgcttta tacgtcgaaa agaatttgag atgccgatat
     1201 tctcactata aggaatatca tcattaagtt tttgttgaat atgctccagc ttgtcttggt
     1261 ccattccttt cccattttca acgattttta acacataatg gttggttgtt tcagttgcat
     1321 agacagtcaa acgattttcc ttttttgatg aatcgaagcc gtgtttaata taattttcaa
     1381 caaatggctg taaagaaagt tttggtaaca ttaattgatc aatatgatca atcagctgat
     1441 agtcaaagtt atgtcgataa cgacgttgat gtagttgcaa ggagaggtcg atattaatta
     1501 attcctcttt caaatcaatt atgtcgggct gatatgtatt attgcgatac aacttgctca
     1561 ataaatatat actgtcgctg atttcttcgt catcattcaa tatggcagtc attcgaataa
     1621 attccaaatt attaaataga aaatgaggct cgatttgata ttgaagtgct gcatactttg
     1681 cttcttgctt caaccaatta ttttcataaa gtgttttttg atatgtagca ttatctaaat
     1741 aaaactgatt gatatgcata gcgatttctt tcaaatcacc gactttgttt tcgatattga
     1801 ttgacttgta gtctcctgta gatgaatgat ttaaggaagt aatgatttcc tgatactgct
     1861 ggatatagtt acgaaaactt ctatttaata attgatgaat agcaagaata aacaaactca
     1921 ttaataagaa aatcatgatt gccagaccta ataaggtttg tgacgaataa tttggatttt
     1981 catatacttg taatgtgtgg ttaccgatag ctatctgttg cgtttttttc catttagaag
     2041 gcgagatgtc ttcagataaa ttgacggaat caataccatt agacaaagaa taattgtaat
     2101 gtgttggaaa gagactcttt tttagcgcgt tgatcgaaac gtctagaatc aatgtagcat
     2161 cgggcatgtc ctcactaatg aaacttaatg aacgaataaa agaaattttt tcgttttttg
     2221 taggtgcgta ggcacttaag tatttttcgt aatctaatga gatagatata tttttaactt
     2281 catcatcttg cacaagtttt tttaagttat ctactaaata ggtaggggag cgatcctttg
     2341 acaaaatatc ttccatgtat tcagcaggtg atgagaggta gtagtcatta aggttttctt
     2401 ggaaatcata attttgatag atctctgtca cgataccgga gagaatacga ttatatgttt
     2461 ccatatagtg ggtgatttgc tgaatgtttt gcaggttttg atttttttct ttttcaatca
     2521 cattgatata ggaaaagaac aatgtgaaac aggcaagtaa agtaataaaa aaaatcaata
     2581 tgtatgtaaa acgcataata acttttgata aatcgttttt cattcaaagt cctcattttg
     2641 ataatctcta attttatgaa tcattatagc acatattaca agtgatgtaa acgctttgca
     2701 aacagggcta cttatgattg tgaatacagt ttccgtttaa aaactattct attgtagagg
     2761 tttaataaac taaaaataat gtataaaaaa ttaatgtaag cgattaacca agtgggtata
     2821 atcaaggaaa cgctgagaga ggagagacaa aatgaagcta aagaaagggt tattaggaaa
     2881 tctaaaaaaa aaccgagcat tgtttattat gcttttacca ggtgcgttat ggttatttat
     2941 attttgctat ataccaatgt ttggagttgt aatcgctttt aaagatttca gaatttcatc
     3001 aaggggattc tttacaagtt tgttcaacag tccttgggtt ggctgggaaa atttcaaata
     3061 tctctttgct tcgcaagatg cttggatcat tactcgcaat acggttgttt ataacgttgt
     3121 aggtattttg ctaagtactt tttgtacagt gtcattagca atcattctga gccaaatggc
     3181 gaacaaacgg ttattgaaaa agatgcagac atttatgatt tttcctaatt ttttatcatg
     3241 ggtagtcatc agttactttg tttacgcatt tttgagtacg gataaaggat taattaatat
     3301 tttactaagc aattttggtg tggaacccat aaattggtat agtgaaccga agtactggcc
     3361 ttttattatt gtttttacga gtttatggaa aggcgtaggc tatggatcca tcatttatta
     3421 cgcgtcaatt atggggatcg atactagtta ttatgaagct gctttagttg atggtgccag
     3481 taaatggcaa cagataagga atgtagtctt gccacattta agatcgatta ttgtcattat
     3541 gttgattttg tctgttggta gtatttttcg agctgatttt ggattgtttt atcaattgcc
     3601 aagaaattcg ggggctcttt atgatgtgac gaatgtatta gatacttatg tctaccgtgg
     3661 attaatgaat agcggagata tcggaatgtc tacggcagcg ggattatatc agtcatcagt
     3721 tggctttgtc ttgattttga ttactaataa gatagtcaac aagttagatc ctagttcagc
     3781 actattttag gaggaatcaa aatgatatca aaattaaaac ggcagatgta cttttcgctg
     3841 ccagtcgaac ggtttttcac aatattacta atcttattta ctcttagctg tatttttccc
     3901 tttattttcg taatcgtcat ttcattaaca gatgaaaaag cattacaact caacggttac
     3961 gaattattgc cggctcaatg gagcactgat gcatatcaat acttaataca agatggcgga
     4021 cagttacttc ggtcattagg cgttacaatt atgattacag tgattggaac gttgattact
     4081 gtttttatga cgggaaccta tgcttatgtt ttatcaagag cctctttccc ttaccggaag
     4141 ttctttactt tttatctttt ttttaccatg ttatttgcgg gagggatggt tccttcttac
     4201 ttagtgatga cgaagatgct tggcttaaaa aatacaattt gggctttgat tctacctctt
     4261 gcttttagcc catacaatgt cattatttta aggacattct ttaagaagtc gattccagaa
     4321 tcgattatcg aatccgctaa aatggatggc tgtagtgagt ttcgagtttt tttccaaatc
     4381 gttttgcctt tagcgatccc aggggttgca acaataggtt tattttcaag cttaggttat
     4441 tggaatgact ggttcaacgc attgctgtat attgacacca acaaattgat tccattgcaa
     4501 tatttgttaa tgaagattca aaattcaatg gaattcttag caaataataa tgacattaca
     4561 ttagcgcagc aacaagcaat tcaaaatagc cttcctcaag aatcaacacg aatggcaatg
     4621 gtcgtagtag ctactttacc gattgcaatt gtttatccgt tcttccaaaa atactttgtt
     4681 caaggattga cgatcggtgg agtgaaagaa taaaaaagaa taggagatgt taaaagatga
     4741 aaaagaacat gcagaaatat ttgattggtg gtgcttctgt actaaccctt ggcctattag
     4801 gtgcttgtgg tagcagtaat ggcgaagcaa ataatgaaac aggaaccaca aaagttactt
     4861 ggtatcaggt tggagaagaa cccaaagatt ttgatgaggt catgacaaaa gtcaacgaaa
     4921 aattaaagga agaaatcaat gtagagttgg atatgcggct gattccggac ggggattatc
     4981 agcaaaagct tggtgtgatg atcaattcag gagaagaata tgatatttgt ttcgtcaatg
     5041 gcacagatta tgtgaattat gggaacaaag gtgctttcat tagtctcaac gatcatttgg
     5101 ataaagagtt gaaagattat gcagctgaat tgaatgaagg atttattgaa ggtggggcaa
     5161 ttaatggcga aacttatgga ttgccagtca ataacgatat tgccacaacg gaatattgga
     5221 cctttaataa agatttggta gatcaatacc aattggatgt agagaatgtt gaaggtgtag
     5281 aaacattggc tccagtatta gaaaagtttg cagaaaaaaa tactgatacg aatattacgc
     5341 caatcggtat gacccgtacc agtgtctgta ttccttatga caatattatc ggtggtggtg
     5401 taccgctagc ggttgcattt gaaggcgata cacaaacagt tgttaatact tatgagactc
     5461 cagaaatgat gacttatttg aaggctatgc atgagtttta taaaaaagga tatattttga
     5521 aagatgcagc cactgttacg acagatccat ttcgaattga tagtaaaaac tggtttttag
     5581 gtcgtaactc aagtggttat caaccattag cacaaaaaaa attagaagct ctagcgggac
     5641 atggattaat tttaaaacca gctggaaaag cttttaaaac gactggattt gcaagagtgg
     5701 caatgcatgc ggtatctgct tcaagtaaac atccggaagc agccttgaaa ttgttgaatt
     5761 atgtgaatac caatcaagaa gtagcggatt taatggttta tggaattgaa ggaaaacact
     5821 atgatcgcaa tgaagatggt tcgattactt taacaaactc tgatgattat tatcaaggcg
     5881 cttgggcatt ggtaaatact tatctagttt caaaaacttc accgattaca gaagaaagta
     5941 aaaaactaga agaagaaagt aaaaagtttc aaaatgaagc accgatgtct cctattttag
     6001 gtttctcttt tgacgcttct gattttcgaa cagaaattgc aagtgtacaa aatatcgtgt
     6061 cacaatatga agtactttta agctcgggaa cagttgaccc tgaacaatat gtaccagagt
     6121 tgttagaaaa actagaaaaa gctggattgg ataaaattca ggcatctgct caagaacaat
     6181 acgatgaatg gcgaaaatct aacaaataat tttttactaa atgaaaagaa tagactaagc
     6241 agttagtggg gggattcaaa tgaacgaaaa caaatggacc aaaggattta attgggtttt
     6301 tcaattgttg ttactaaact ttacttattt tatactaatt tgtttaggat ttggaatttt
     6361 aggttttttc cctgctacga tagctttgat tgctggcgtt gtgaatcgaa aaaaatatgg
     6421 cttgcaagag tctgtgaagt tcatgagtaa aatctataag aaaaattttg ttagatataa
     6481 tctatattgc tggtcagcaa ctttgttgtt ggccggcaat attactacga tctggctttg
     6541 ttggcagcaa agcgcaccat tgttttattt attaagtaca ttattatcgg taattacact
     6601 gttcctagtc tttgcaatgt tgaattgggt cgtgatcaaa agtcttgggt tgtggatgat
     6661 gggagaccga ctgattgact atttgctttt cacagcaacg atgttgccaa cggtagtcat
     6721 gcaaggcttg gtgcttggaa tttatttccg aatattgatg attttgcctg tgatttttct
     6781 gtttggcgga acagctgtaa cggtaatatt gattagtaaa ttaactgaaa gagcgttagc
     6841 aaagttgcct tgtcagatac aatcgcacta attttttcac aaaaaggagt atttctttct
     6901 atgaagttaa ctgataaaca agtagctaat atagccttta tccagcaact tgagattcca
     6961 ttgacacaaa aagatgaaga acaaatagac ttgattttta ctgacacaaa ggctaatgtg
     7021 cgacaaatca agattactaa aaatgagcag caacgaattt gcttgtctta ttcacatgaa
     7081 gacttgctgt atcgaggatt gtttatctgc cttccacgtt ttttagctca aggaggagag
     7141 atgccataca ttgaagaaac ctcgatcatt gagaccagtt attcattaga tgcttctcgt
     7201 aatggcgtga tgaataaaga gactctaaaa aaattcattt cttacttagc gttgatgggg
     7261 tacaacacat tgtatattta cacagaagat acctatgaag tagaagacct tccatatttt
     7321 ggttaccttc gcggagcgta tacaaaagaa gaagtgaatg aaattgttga atttgcttct
     7381 ggttttggca tagaagtagt tccatcgatc cagacccttg ctcacttgac ccaatttttg
     7441 aagtggtatg ctcaacaaga actgatggat gatcaaaata ctctactgat cggtgaagaa
     7501 aaggtttatg cggcaatcga aaagatgatt attgcctgca aagaaatgta tcagacagag
     7561 cgtattcatt tgggaatgga tgaagcctat caagcaggtc tgggacgata tttaagcctg
     7621 tatgggtatt gtgatcggac aactttaatg attcaacata tggaccgtgt gttatcaatt
     7681 gttgataaat ataagatgaa accaatgatt tggagtgatt tcatttataa gctattagat
     7741 gaaaccggta aagataggta ttacaatcct gattttaaac tcaaagcatt agcagctaaa
     7801 ggattaccag caggattaac ctatgttcat tgggactatg gttcagatga agtttcctcc
     7861 tatgagaaag taattgataa ccatttgaaa ttttgtgaca ttgatcatta cgttttagct
     7921 agtggtgccc atatctgggg gagactagcc ccgaatcatg ggaaaacaga agcaattgtt
     7981 tctgcagctg taaaagcttc taaaaataaa ggtgtacgtt ctgtgatgtt aactacttgg
     8041 ggagatgaag ggcaggaaac agagcattgg cattccttaa ttggggcatt aaaattagca
     8101 gattatgtct attctgaaga agagtggaat gattttgagg aaatctacga tcgtttatta
     8161 ggaacaggta gttatcaatt aatgtccgaa ttacgatatt tcgatgaagt ggataccatt
     8221 acaaataaca acttacaaat ggcttccgtg agtaaactgc tactttggca agatccgtta
     8281 gttggtatat atgactttca tgtagccgct cataacaaaa ttgccactag aacaattggt
     8341 acttattatc atgagttagc tcagaaatta gatatgaata aaaattggaa tccaaaacaa
     8401 ccaattttaa caactattcg tcaacgatat atacttttag ctgaaatttt agcacaaaag
     8461 gcagaactag gcatctattt acaacactgc tgtctggtag aagaccttgc aggattaatg
     8521 gaattagcga atcggtgtga agcactagcc aatcgagtaa atgaattggc atctattcat
     8581 gaaataattt ggtatcaaac atacaaaacc tatggttggg aagtattaga acaacgatat
     8641 gctgcattag ctcatcgtct gaaaaccgtt gcgcgtcgta ttaaaaatca tcttcagggg
     8701 caagatgccc tagaggagct tttggaagaa cgattaccgt tttgtcaata tcaagtgccg
     8761 cttgaaattt ctggctttaa ttatcggcaa agctcggtgt caggctacaa ttaacgaagg
     8821 aatcttcccg ttgaacggac cacttgctca tctaatggga aagtggttcg ttttctattc
     8881 ataagtagct taccactaac tcttttatta agaagagaaa aaaagtagga agtgagaaaa
     8941 tatgttatcg gactattatc aaaatctgtc agttataagt gtcaatacag aaccattacg
     9001 taattattat attccttatg caagtaaaga agaagcaaaa caagagttag aaagggaaaa
     9061 aacgagccag tttatcgatc tcaatggtac atgggatttt cagtattatc cttctgtcca
     9121 acagttaccg gaaaattttt ttcaagaggc ctttactgaa agctatgata gaatttctgt
     9181 tcctggagtt tggcaaacag agggatttga cttacctcag tatatcaatg tatcttatcc
     9241 aattcctttt gatccgcctt atgtacctaa tgatacccct tgtggtgtat atcggcgtca
     9301 gtttcaaatt gagcaaacag atgcacgctt ttacttaaat ctagaaggag cagatagtaa
     9361 ttgttttgtt tggataaata gaaactttgt aggctatgac caagtgagtc atagtacatc
     9421 tgaatttgat gtgaccaagt atttacaggt tggggttaat gaaattcaaa ttctagtgat
     9481 gaagtggtgt gatggtacct actttgaaga tcaggataag tggcgaatga gcggtctatt
     9541 tcgtccagtg tatcttcttc aacgaccaac tatgcatctg acaagttatc aactacggac
     9601 gttgactgat cctaaaaaag gaagcggaac attaatggtt tcttttcaac gatttactcc
     9661 tattgaagta tcgttaccta tttcttacga attattccat cctgatggac gtctcatagc
     9721 gaaaggggag tgttactcgg aacaacaaat aaatttggat gttgaagatt gccttttttg
     9781 gacagcagaa acgcctaaat tatatacatt acttttaaca tgtaatggtg aatattttaa
     9841 agaaaaagtt ggctttcgag cactgacgat caagaatcgt aagttattgc tgaacggaac
     9901 acctattaaa ttatatggag tcaactatca tgacgtgaat cagcaaggca cgccagtgat
     9961 gacccgagag cagttacgtg cagatttgtt attaatgaaa aaattaaatg tgaatactgt
    10021 acgaacggca cattacccta aatcaccaga tttttatgaa ttggcggatg agattggtct
    10081 gtatgtaatc aatgaagcag atttggagtg tcatggtgtg gttaatctat atgatactga
    10141 agcgaattat caactaattg ctaatgatcc gacattcgaa aagccgatta tggaccgagt
    10201 gatccgttct attcttcctt atcaaaatcg aagctctatc attatgtggt ctatggggaa
    10261 tgaatctggt tatggagtaa attttgaaaa agctcaagct ttagcacgga aattggatga
    10321 ttcacggttg attcataatg aacgctcgat tgaaccagtt attgaccaag tgaatgattt
    10381 tagtaacttg gatgttatta gtcaaatgta tcccccactg gaagatgttc atcgtcattg
    10441 tcaaagaagt gatactgaaa aacctttttt tatgtgtgaa tatagccacg ctatgggcaa
    10501 cggtcctgga gatcttaaaa cgtacttcga tttgattcaa cagtatgatt cctttcttgg
    10561 cggttgtgtc tgggagtgga ctgatcatgc aatcaataga ggaactttag aaacgccatc
    10621 ttatttatac ggtggtgatt ttggtgaaaa atatcacgac ggcaattttt gcgtagatgg
    10681 tttattgacc ccagatcggc aattaacagt aggtgcattg gagtaccgta atattcattc
    10741 gcctattcaa ctgattgctg tagatgagga gctgcaagaa gtaaccttga gcaatataac
    10801 tgcttttaca gccggttcag catttaacct ttctcttgaa tggtatatag atggaaaaaa
    10861 aacagtttat gaagtaatga atcctttcat acttgagcct ggcaaaagtt gttcattgcc
    10921 catcacagta cctacaacga ttcatggtgc tcatacggtt gctgtttcgc tatctgttta
    10981 taaaaatgat gagctttatg gacagcaaca gctctttctt aaaaaagaaa aaatgagatt
    11041 gccaagaact gctcatcata atgatgcacc acaaatttca gtaaaaggca cagagctgat
    11101 aatcaaagga gaatccttta tttatatata tcaattagca aaagctgcgt tcacttctgt
    11161 aaagatcaat caaaaggaac ttttggaacg tcctactcaa ttttcaattt ggcgtgcacc
    11221 aacggataat gatcgtaact tagtaaacaa atggaaagca gctggatatg ctgaaatgtc
    11281 tttagaaatc agaaaaaaag actggaaatt cgtagatgat catctcctga tcgaaacgtc
    11341 ttatcgagga gttattccag gattacagcc aatttttaca atgcacatac agttgaaaat
    11401 aaatggtgca ggcgtaatca accaaaaagt atcagtggag aaaaatccga tttttccaga
    11461 tttccctcgc tttgggatgg actggccgtt gaatgatact ttttcgaagg tttcttattg
    11521 tggctatggg ccgtatgaaa gttatcctga taagcactat gctgcgatgt ttggtattta
    11581 tgagatggag attgaggaat ttgatcagcc atatatcaaa ccacaagaat atggtaaccg
    11641 ctggcaatgt gattgggtat caatcaaaaa tcatcaagga tctaccatga cggtttatgg
    11701 aaactctaat ttttctgctc tgccttatag tcaagctcaa ttaaccgaaa agtcacacca
    11761 ttatgaatta ataaaagaaa atagaacttg gttcaattta agttataaac aaaatggtat
    11821 cggctcacac agttgcggtc cttatttaga agatcgtttc cgttttatgg aagatacttt
    11881 tgactggaat atagtgatta ctttcaaagg agaaaataga tgaaagaaac aaagatcaat
    11941 caagtcaaaa aaaaattatc tcgttatcaa gaagttgttt atcgaaacca ttgcttaaaa
    12001 tggtcaaaaa ataagctgaa tgttcgtttt aatggattat tttatcgttt cgaacagtta
    12061 gaagaagtct ggaaaccggt aacgaatttc caatcatgtc ctgtacagct cataaatgat
    12121 ccaaaagaaa catacgattt atgtataaca cacctttata accagcattt gaaaaatgac
    12181 ggattttatt taaaggttac taatgacaat caacttacga ttgaatcagc aaaccttcga
    12241 gggtttcaat atgctatgga agccttatta gagagttttt tttcggtagg agatcaatta
    12301 ttattaccgg aattgattct gaaacatgaa ccatcagtga aaattcgagg aatcattgaa
    12361 gggttttatg gtatcccctg gacacatgaa atgcgcctct ctcttttttc atatatgaaa
    12421 gataaccaat tgaatacttt tatgtatgcc cctaaagatg atgaattatt acgtaaaaaa
    12481 tggcgtgaac tctacgatgc gcaagaatta gacaaattta aggagctttt atctgctgct
    12541 gaggctagca atatcgattt ttggtactta attagtcctg gaaatgatat tgatattacg
    12601 tgtgaagaag acattcaagt tcttctgaaa aagttagaac aactgattga gcttggcgta
    12661 tttcaatttg gtctattaat ggatgatatt gactatcaat taaaaggtgc tgctaaacgc
    12721 cgttttcgag agccagcatt cgctcatgcc tatttagtga atcgagtaga ggaatatctt
    12781 agcactcggt tgtccaatca tgaattagtc atttgtccta cagaatatga taatcgtcat
    12841 ggttctcgct atttagcaac gttaagtcaa gaaataccag aacaattacc gttcttttgg
    12901 actggcccaa gcacgttagc ggcgtcaatt tctacagaag agctgcagga aatggcttct
    12961 gtctatcaac ggccgatgct gatttgggat aatattccgg taaatgatta tttagaggat
    13021 aaagaactat tgttcatgag tccatatgag aatcgaacac cgaatctttc taaggagaga
    13081 taccaggtta caggagtagt ttcgaatcca atggctcaat tagaagcttc aaagtttacg
    13141 atcaatagta tggctaatta cttatggaac tgtgagcgtt ttgatccttt ggaaacttgg
    13201 acgtcagtcg ttgaaaaagt agttggaagt aaactacaac cagcttatct taccttaacc
    13261 aatgcttttc caaatcatta cacaagcagt ctgctttcag atgaagaatt actgctagcc
    13321 aaagacccag aatgggtcac aaaagaaatg gctgctttag tccaagcagt aaaatggatc
    13381 cagcaagaac aacatacatc tagattgaga acagaattgg agccttggtt acaagcaatc
    13441 acagaaagtt ggactttctg gcaagaatgg caattaaaaa aagatccaaa agaagcagaa
    13501 gaatggttgg ctaagaaaaa aacacaccgc ataggacgtg atttagtaac tgcccattta
    13561 gagcaaatta tcaaaagtta gatatcttaa aaaataaaaa gcaagaaaaa ggagaacaaa
    13621 atggtcaaga gattaattag tgcaaactac agtgatgtca agaaaatgac gtcaaaagag
    13681 ttgaagcaat ccatcaaagc gagtgaagga cgggtgattg tttgtgaaac ggttgctaat
    13741 actcttccac aatctggaga aattacgaat gcagaagttg cgtcagcatt tggtgcagat
    13801 ttgattcttt taaatggttt cgattgttat cgtcctgcag tttttggact accaaatgtg
    13861 acgattgaga aggtttttga tctacagcaa acgattgaaa acccgattcc taaattaaaa
    13921 gaactagtcg gtcgtcctgt gggaattaat ttagaaccca ttgcccctaa aacaaatatg
    13981 atgagtgaga aagtagaaat cagccaagga agagcaagct ctgttgaaac gatacaaaaa
    14041 gcagaagaac taggagtcga ttttatttgc tttaccggta atccaggcac tggagtaacc
    14101 aatttagaaa ttactaaatc cgtaaaaatg gcaaaaaagt atttttcggg attgattatt
    14161 gctgggaaaa tgcatagcgc cggctcagaa gagccagttg tgaataaaga ggcgattatt
    14221 tcttatgcgg aagcaggcgc agatatttta ctagttcctt cagtagggtc tatcccaggc
    14281 tttttagagc aagacctaat tgaagcggtt cggttggcaa aaaaatatga tttgttaacg
    14341 atgtctgcaa tcggtactag tcaggaaagt gcccgaccag aaacattgcg gcaattagcc
    14401 attatgaata aaagttgtgg tgttgattta caacatattg gagatgctgg atacggggga
    14461 ttggcaccag cagaaaatat tttcgaaacc tcggttgcaa ttagaggaat gcgacatacc
    14521 atcaactgcg ttagccgctc gatcaatcgc taacaaaatt aaataaaaaa tggttaaatt
    14581 gattatttaa gtgggaacat acttgagata tcagctaact tctgtcactt taaaaaaggg
    14641 actgtgacag aagtgaaaat agacaaaaaa tatccgaaca agagactagg atagcagaaa
    14701 aactatcgtt ttgcaacgag gaccaactgg accgtaggag caattctctt attgttcgga
    14761 tatttcgctt gttcaaggac ttatgtctgt agtgcccttt tttggtagga aaaatggtag
    14821 ggaaccatat catttttaat gaaaatcaag catttcaaaa gt
//
LOCUS       DAZKSR010000073        14798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000073 DAZKSR010000000
VERSION     DAZKSR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.073"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG8XG_002068"
     CDS             143..400
                     /locus_tag="ACG8XG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773972.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="ACG8XG_002069"
     CDS             complement(632..1063)
                     /locus_tag="ACG8XG_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFQ0773973.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(1414..2112)
                     /locus_tag="ACG8XG_002070"
                     /pseudo
     CDS             complement(1414..2112)
                     /locus_tag="ACG8XG_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            2191..2367
                     /locus_tag="ACG8XG_002071"
                     /pseudo
     CDS             2191..2367
                     /locus_tag="ACG8XG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2543..3112)
                     /locus_tag="ACG8XG_002072"
     CDS             complement(2543..3112)
                     /locus_tag="ACG8XG_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0773974.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(3186..3509)
                     /locus_tag="ACG8XG_002073"
     CDS             complement(3186..3509)
                     /locus_tag="ACG8XG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773975.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3663..4475)
                     /locus_tag="ACG8XG_002074"
     CDS             complement(3663..4475)
                     /locus_tag="ACG8XG_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773976.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4802..5677
                     /locus_tag="ACG8XG_002075"
     CDS             4802..5677
                     /locus_tag="ACG8XG_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0773977.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            5691..6014
                     /locus_tag="ACG8XG_002076"
     CDS             5691..6014
                     /locus_tag="ACG8XG_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773978.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            6011..6763
                     /locus_tag="ACG8XG_002077"
     CDS             6011..6763
                     /locus_tag="ACG8XG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0773979.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6753..8924
                     /locus_tag="ACG8XG_002078"
                     /pseudo
     CDS             6753..8924
                     /locus_tag="ACG8XG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(9036..11207)
                     /gene="copB"
                     /locus_tag="ACG8XG_002079"
     CDS             complement(9036..11207)
                     /gene="copB"
                     /locus_tag="ACG8XG_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0773980.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(11227..13413)
                     /locus_tag="ACG8XG_002080"
     CDS             complement(11227..13413)
                     /locus_tag="ACG8XG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0773981.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="ACG8XG_002081"
     CDS             complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="ACG8XG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0773982.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13635..14075)
                     /locus_tag="ACG8XG_002082"
     CDS             complement(13635..14075)
                     /locus_tag="ACG8XG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0773983.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(14149..14688)
                     /locus_tag="ACG8XG_002083"
     CDS             complement(14149..14688)
                     /locus_tag="ACG8XG_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0773984.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4284 a         2991 c         2564 g         4959 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaaag tgaactgaac cccaaaagtt
     1321 gaactattta atggactgtt tccgatattc tactggagat agtccattaa atagttcaac
     1381 ttttggggtt cagttcaaag agggggggat tttttatcgt tgtggaatat ttaaatacca
     1441 acgcttgtca tgaaaatctt gtgctccacc cttagtattt ccttctggat cgtttgtcgc
     1501 acgcatcatg acgtatactt tcttattagg gaaatcacgc atattgaaag atacatgata
     1561 gccaacattt cctaaagtat tataagcttg atttacgtct ggtctataaa tcccatcagc
     1621 tcttactcta gctagttctt ttccagtatt gtagtccata atgaaaatat actcgtattt
     1681 ataattagca atgtgccatc cagctacatg caagtttgcg ttttcgattt ctccgaactg
     1741 atcaatgtgg gcgtaatttg ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt
     1801 tggatcaatt actggtttat cctccgaagt agttggatta tcatccgtaa aaccatgagc
     1861 caaatcatag gctagttttt ctttacttac acccatttca gaaagataac cgtaaggatc
     1921 tgtatgatcg ccccaaatat tttgtgttac ccataaatgc gatttgattc ctggttggtt
     1981 ataaggagtg tctaatgtta atggaatacc atatttcatt gctgaatctc ttgccaattc
     2041 aacgtatact ttatagttct tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat
     2101 ttgcacagga cttttggctc acgaggttgt cctactctac atcttgctga tagatatgct
     2161 gaaaggaaaa caccaacatt atgttttgct ttttcagcta gaacgagaca agcaggaata
     2221 cctttagaag atatcaaagg ttttttaggt cataaagatg tttccactac acaagtctat
     2281 gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa
     2341 gagcaaataa aaaagcactc aaattgagtg cttttttaag ctcactatca ctaaaactat
     2401 cactaatttt atttataagc gaacatgttt ctatcactga ttttttaaat ctagcgaaaa
     2461 acccttatct taaaggcgtt cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc
     2521 tagaagtgcg aacatggttt tcttatagct catgtagaaa aattttcttg atgatgattt
     2581 tatccatcaa ttgagattga ctgttttgct ctcctatgat ttcagctatg attgtccgat
     2641 tttgcggttt tcttcttaac tggtagttga ttttcccagt ttccatcaac tcgctaagca
     2701 tatccacgac tccgccaaat ccggtctttt tcgtaataaa acctagtgct accattcgtt
     2761 catctagatt ttttgctgta atcgtcacac cagtaatgag cgagtgctgt aatgtttggg
     2821 ccagctcttc catcccttca gcattggata cgacggaagc tgttttgtta ataggttctt
     2881 cgtttatttc ttttggttct ttcctctcca ctttcactgt ttgacgaagg acatctagtt
     2941 cttgcaagag cgtttcgtat tgttcttggg tcagcatcac gccagctact ttttcacgat
     3001 taaatatata tacgccagtt cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc
     3061 gtttcacttc tgtgatcgaa gatgtgggaa cttctaattt ctttaattcc aatggatttc
     3121 ctcctttact aatcgctttt tattatagca cagactgact tgttttgatc ttttttccaa
     3181 aggttttatc ggtttttgcc taaccagtaa ttgatatcgg taagaagtct ttcgatacca
     3241 aatgaaggat ggtacagata cgttttttga tcttcgttat ggtctagtgg aaaaaaatga
     3301 tcgatgatca ctagatctgc ataccgaggc ggcttatttc gagtacatac atgtatagag
     3361 atccgttttc gatgtcttag cgttttgttc agtaccccag caagtcttct tgcttcttgg
     3421 tgttcatagc gaaaaaaaaa gacgattttc acttcaggag ggtggacatc taaataccct
     3481 cgttcctgga gtgttttcag taaaagcatc ccccactcta ctgcggggtg tgcaagtgct
     3541 tgttggatcg ctagccgaaa ttgatatagt ttatctttgg aattgggaaa tttttctaat
     3601 gtttcctgat aataacgaac ttcttctatc agccggtcat ggataccaat tacgtaacga
     3661 agtcattctt ggtaaagtac ttttgtcagc tttgatttgc tttcgctctc aaattcgcta
     3721 ttcagccaaa acagcgaatc aagcttttca gtcacaatcg tcaatttccg atagtcttgt
     3781 gtctcttcta tccgtttcca ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt
     3841 gctagataat ttttttcttt tattggcaaa atctgctcct ctctaaatag caattcagga
     3901 aacaattcaa agaatagtgt tcgactgtct cctaaaatct cgatttctgc cggatccttt
     3961 ctttgcttca ataattggat gaacatctca gaaaaggact gctctccatc atctgcaaaa
     4021 aaatgcggaa aatctttcaa aaagcaaaac agcagatatc ttacttttct ttctgaacca
     4081 aagatctgtg cttttttata agaaaaagaa aggtggtact cgccaaacca ctttctcaat
     4141 cgctcttgcg tagaacgatc gtacttctgc tcgtaaggtt cattcaatag cagatttttc
     4201 agtaaacaaa acgggggtga ttggatcaga aaaacaaaca agagataagg ttcgctatat
     4261 ccttgatcat aaactaactg aacggttttc ttatgaagaa tcagctgaat gggacagtcg
     4321 ctttccgcca aacgtttcag atgccgatcc actgttttaa catcgatttt cagttcttca
     4381 gctaaatcag cttttcgcca gatcaaatct ttcattagat tgattagtga aaataaattc
     4441 tgtgcatttt ttgataatag tttttgccat ctcatcgatc atttctcacc tgatttctaa
     4501 acatattgtt tgatcaaaat gggttcactc ctaaaaagat aaaaaactcc ctttctattt
     4561 atttaaatac gcaaatcttc ctcattattt tttattttac tcaatttaat cgtattctca
     4621 tttcaaaacg aatgtacttt aaaaaaacat aataagatca ctttgcttgt aggatgtatt
     4681 cccttccatt acaatgaaat aaatagcgat tttatgagaa ggtgatgtga aaatggaaga
     4741 aaaaatagaa gaatccaaac aaaaaatcga tcagttgata taagaattag aaaaagcggt
     4801 catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt
     4861 cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc
     4921 tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct
     4981 tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt
     5041 taccacgatc ttgttagcag atgagatcaa tcgtacgact cctcgtactc agtcagcact
     5101 tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa
     5161 tcattttttt gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc
     5221 ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga
     5281 cgaacttttg ctacttttgg ataaattcga aaaagtgttg gataacttaa atgaagtatt
     5341 gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga
     5401 agtggcatct tatgccttgc agttagtgtc agcaacaaga agtcatgctg ccatccagct
     5461 tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat
     5521 ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc
     5581 ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaagaaac aaatcattca
     5641 tactcttgtt gcacaaattc ctattcctgt gaggtagatt gaggtagaca atggaaaaaa
     5701 tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg
     5761 ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat
     5821 catggagctt gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc
     5881 agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa
     5941 ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa
     6001 acaagagatt ttgattctta caaataaacc gcaagaagtc caattgcttt ggcagccaaa
     6061 agaaagagga tgccatgaaa cattaactgt catttttaca gcctatgata gtctaggact
     6121 tttcagaaaa agtgcaaaaa gagagatctc ttttcctgtg actgttctgc ctgctttttg
     6181 taagattcaa gcagaaaaat tacagagagt tctggaaaaa aaacagcagc tgaacgcgta
     6241 ccaaagaggt cttgattttc gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg
     6301 aatcgattgg aaattgtctg ctcatactca agagtggtta taccgtgaat acgattatca
     6361 agaagaagaa tgttctcctc ttttttgctt ttggggttcg cccagtccaa aatttgaacc
     6421 tctgctagac ttgtattttt ctttatggca tttgagaaaa gaaaagcagc cggaacttct
     6481 gatattaggc aaccgcgcct ttcatggcaa agaccccggt catacctatt ttgcttcttt
     6541 ggaagctggc gatgagaaag cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat
     6601 cgtcctcttt gttcctgaga attctcctga agtctcagaa gctattgaaa cattgtcaaa
     6661 agatcgaacg gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga
     6721 caagatatgt tcattgccag gaggtgaatg gattgacgat taaacgggaa aaatggctgc
     6781 gtggcgttta ctcctttgtg atactaagtg taggtgctcc gatatttgta gtagctaatc
     6841 aattgcaaaa cagctacctt cttatcctaa ctattggtgt cctatgcctt attgtctgct
     6901 gtattcctaa aaaatgggta cgtttgctgc tttctttgcc agtaatgata ggttgtttgt
     6961 ttttatattt tccttcgaag acattttcca tattatggat cttacagttt ttttcgtcag
     7021 tcacagagga attcttccat ctatcccaag gtaaacttta ttatttgcca gaaaagacag
     7081 ctttttttct cataatgttg ctgatctatt tatttattac attgaccgtg gattacgact
     7141 attggtacgc accctttctt tctttgatgg cttacttcat gatgcttacc gtttttcgga
     7201 aaaaagattt actgtacgaa atgattgcgg tagtaactgt tttgttcgtg tatctggcag
     7261 caaggcaatt tttctccatc cggttgttgt cgggaaacac tcgattccag tcattgacta
     7321 cagttctgct tatttgtttc ttaactttta gcgctatctt gcctttatat cttccgaaag
     7381 tacaccaatc tctcgaggaa acgagtgaac cgatccgaga atatcttaat gatgaagggc
     7441 tttatgacac actccatgag tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga
     7501 atgatgaaca gcttggtgga cctttatatg ataatggcga agttgttttt acagcgaacc
     7561 aaaaaggaaa tggctattgg aagatcgaaa ataaaaatca ttacaccggt tctggatgga
     7621 tagaagatca ggacgcaccc atccaaccaa tccaagaatt ccctgttgac atcacgagct
     7681 accaaaccgt tatagaagac aaagaaaaca ttacattgac cctaaaaaaa caagttttct
     7741 taccttatcc ttatggaaat actaccttga ctgatttttc attagctagt ggacaaacgc
     7801 tttactattt gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg acacgttctg
     7861 caactttaca aacagaaagg ttagttttta cgagagaaga attaaaaagc agtcagttaa
     7921 aagctgatga tttagaaaat cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac
     7981 gtattgagga cttggcggtc gaattgacca aaaatgaagc aactttaatt cgtaaagtcg
     8041 aagctgtcta acagtattta aaaacaagcg gaaatttccg ctattccaaa acggataccg
     8101 ggcatactcc aaaagaacac gattatgtgg attatttcct ctttgattca aaagtcggct
     8161 attgtgacaa cttttcaact tctatggtcg tccttcttcg aacacttggt atcccagctc
     8221 gctggacaaa aggttttaca aatgggctca gaacaggcac agataatcaa gggaatgagg
     8281 aatacagcat tctcaatagt catgctcatt cttggccaga ggtatacttt cctggatttg
     8341 gctggcttcc atttgaacca accccttcat ttgccaatcc aatgggttca caaccagttg
     8401 ctgaaacaac cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg
     8461 cagaaactat ttccagtgag agatctgagg tcattccatc agaagaaaca acactcacac
     8521 cagcttataa aatcgtctta ttcagccttg gagtgtgttt atccgtcttg ttgttctttc
     8581 tattgttatt cagaaaatct ctgcgctgga caagttatct tatctatctt catttttttg
     8641 cttctactaa tacgattgca tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc
     8701 gcaaacccgc agaatctttg caggaatata gcctgcgaat agaacaagaa ttcaagctag
     8761 atctgcgttt cattcgatta actactcaat atgaagcaga aatatatgga cgacaagcag
     8821 atccggtaaa attcgataaa aaaacctatt tagaaataac gcatataata agaaaaatgg
     8881 tatacaaaaa aattctgaca aaagggagcc gcttcaaaaa ataagataaa atttctggag
     8941 aatctacttc tggaatgccg agaaccagct gattgagtat cgattactcg aaaaaaaggt
     9001 tgtgacacgt tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca
     9061 tttagagcta caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat
     9121 atgataccaa ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct
     9181 gccccccacc agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa
     9241 tgcaaaatat cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata
     9301 tctgtccctg caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg
     9361 atcccatcac ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct
     9421 tctttatcat ctggtaataa accgccataa tattcttcga tccctaaata atctgctact
     9481 gcttgggcag cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc
     9541 aatgttcgga taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca
     9601 gctagatgat tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc
     9661 aataatgctt gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc
     9721 acttgttgtt ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca
     9781 gcttgataag gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga
     9841 tgattggcat tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca
     9901 tcatcaagta cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg
     9961 tccagcatga tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt
    10021 aatccgtttt tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct
    10081 agagcatgag gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga
    10141 tctgtcaaga acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac
    10201 agccattttg ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt
    10261 cgcaccattt ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt
    10321 tcgatcgttc catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg
    10381 attcctcttg attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt
    10441 acaccatctg ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact
    10501 tctttcaatg aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc
    10561 agtaattctg ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc
    10621 cagtgaccta gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg
    10681 tgtgtatgtg gattgagcag atttgcgata aacgaataga cactataaat atatgcaact
    10741 gtaatcccca tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta
    10801 gctccactta aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg
    10861 agccaatcag aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa
    10921 aaaagaatga ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc
    10981 atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt
    11041 tgtttgctgt ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga
    11101 ctatggtcat gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact
    11161 acttcttttt tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc
    11221 cttctcttac tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga
    11281 actaaacgcc atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg
    11341 aataccaatc gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt
    11401 caacctagac aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac
    11461 atcggctgtt tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa
    11521 tgctaaggcc ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt
    11581 ctgaagtttt tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt
    11641 tggatcaata ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag
    11701 cataaaaaca tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt
    11761 gatttgatca gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac
    11821 tgtttttccc tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt
    11881 caattcaatt aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct
    11941 gattccagca cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag
    12001 ttttccgtat tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata
    12061 aaaaatagat aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt
    12121 aatcgttcca gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc
    12181 tccgccttta attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt
    12241 tggagtcgct aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat
    12301 caatgcgagt tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat
    12361 gactaatacg atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc
    12421 tttgctccct tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc
    12481 gacttgtgta acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt
    12541 gtcatccgga ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc
    12601 actgcttcct gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag
    12661 ttgatcacca acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac
    12721 ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt
    12781 tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc
    12841 aaaataaaga tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc
    12901 agccgatgtt ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg
    12961 gtacgcacct ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa
    13021 ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc
    13081 aatcattgac aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat
    13141 ttttttcaaa tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa
    13201 aattgcacca tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc
    13261 atatttgaca cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg
    13321 tccgttcagc tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac
    13381 gaacgtatcc attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat
    13441 ccattgattg cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta
    13501 ccgtttgctt ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt
    13561 tcttctactc ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc
    13621 attttttctt cctcctattg atgttgttta cattcacatt gacctgggat acagttacat
    13681 tcgatcgtat ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct
    13741 tgagtcaaag tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag
    13801 atatgggaaa aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga
    13861 taataaatga atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga
    13921 cccagcaaag ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg
    13981 atttcttggg ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa
    14041 tcactgatct ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa
    14101 tgtaatcatt atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt
    14161 gctctctatt ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat
    14221 cagtttattt cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc
    14281 ttcaaaatgc cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa
    14341 tgattcaggc atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac
    14401 tactcggtag atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt
    14461 aatcctttcc tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac
    14521 cataaacaat tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt
    14581 atgccataaa aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc
    14641 gagggtcgat tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca
    14701 cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact
    14761 gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataa
//
LOCUS       DAZKSR010000074        14182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000074 DAZKSR010000000
VERSION     DAZKSR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.074"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8XG_002084"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8XG_002084"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0773985.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACG8XG_002085"
     CDS             complement(2262..2513)
                     /locus_tag="ACG8XG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0773986.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACG8XG_002086"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACG8XG_002086"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0773987.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACG8XG_002087"
     CDS             3463..3681
                     /locus_tag="ACG8XG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0773988.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACG8XG_002088"
     CDS             complement(3970..4482)
                     /locus_tag="ACG8XG_002088"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0773989.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACG8XG_002089"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACG8XG_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0773990.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACG8XG_002090"
     CDS             complement(6935..7378)
                     /locus_tag="ACG8XG_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0773991.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACG8XG_002091"
     CDS             complement(7379..8167)
                     /locus_tag="ACG8XG_002091"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0773992.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACG8XG_002092"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACG8XG_002092"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0773993.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACG8XG_002093"
     CDS             complement(9282..9869)
                     /locus_tag="ACG8XG_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0773994.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACG8XG_002094"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACG8XG_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0773995.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="ACG8XG_002095"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="ACG8XG_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0773996.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="ACG8XG_002096"
     CDS             complement(12786..13721)
                     /locus_tag="ACG8XG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0773997.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13907..>14182
                     /locus_tag="ACG8XG_002097"
     CDS             13907..>14182
                     /locus_tag="ACG8XG_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0773998.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         3957 a         3158 c         2502 g         4565 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataacc cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac
    13861 tcaaattgta tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca
    13921 tcttaccata gacgaactga caatgataga atcatattat cttcaacata ataagccggt
    13981 tgaaatcgct aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt
    14041 caagcaaggc aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg
    14101 tggtagaaaa gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac
    14161 tcttggttgg acgcctgacg tc
//
LOCUS       DAZKSR010000075        13917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000075 DAZKSR010000000
VERSION     DAZKSR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.075"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8XG_002098"
     CDS             131..505
                     /locus_tag="ACG8XG_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0773999.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8XG_002099"
     CDS             complement(619..1248)
                     /locus_tag="ACG8XG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0774000.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8XG_002100"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8XG_002100"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8XG_002101"
     tRNA            1668..1741
                     /locus_tag="ACG8XG_002101"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8XG_002102"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8XG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774001.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8XG_002103"
     CDS             2338..3666
                     /locus_tag="ACG8XG_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0774002.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG8XG_002104"
     CDS             3868..4701
                     /locus_tag="ACG8XG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0774003.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACG8XG_002105"
     CDS             4717..5454
                     /locus_tag="ACG8XG_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0774004.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACG8XG_002106"
     CDS             complement(5609..6577)
                     /locus_tag="ACG8XG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0774005.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACG8XG_002107"
     CDS             6809..7642
                     /locus_tag="ACG8XG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0774006.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACG8XG_002108"
     CDS             7676..8515
                     /locus_tag="ACG8XG_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0774007.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACG8XG_002109"
     CDS             8541..9101
                     /locus_tag="ACG8XG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0774008.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACG8XG_002110"
     CDS             9300..11021
                     /locus_tag="ACG8XG_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0774009.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACG8XG_002111"
     CDS             11186..12328
                     /locus_tag="ACG8XG_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0774010.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8XG_002112"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8XG_002112"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0774011.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAZKSR010000076        13905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000076 DAZKSR010000000
VERSION     DAZKSR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.076"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8XG_002113"
     CDS             168..362
                     /locus_tag="ACG8XG_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774012.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8XG_002114"
     CDS             631..930
                     /locus_tag="ACG8XG_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774013.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8XG_002115"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8XG_002115"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774014.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8XG_002116"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8XG_002116"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0774015.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8XG_002117"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8XG_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0774016.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8XG_002118"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8XG_002118"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0774017.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8XG_002119"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8XG_002119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0774018.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8XG_002120"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8XG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0774019.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8XG_002121"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8XG_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0774020.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8XG_002122"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8XG_002122"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0774021.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8XG_002123"
     CDS             7521..8291
                     /locus_tag="ACG8XG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0774022.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8XG_002124"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8XG_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774023.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACG8XG_002125"
     CDS             9959..11194
                     /locus_tag="ACG8XG_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0774024.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACG8XG_002126"
     CDS             complement(12193..12453)
                     /locus_tag="ACG8XG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0774025.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACG8XG_002127"
     CDS             complement(12480..13781)
                     /locus_tag="ACG8XG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0774026.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4482 a         2837 c         2180 g         4406 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttat
//
LOCUS       DAZKSR010000077        13810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000077 DAZKSR010000000
VERSION     DAZKSR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.077"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..2235
                     /gene="acm"
                     /locus_tag="ACG8XG_002128"
     CDS             70..2235
                     /gene="acm"
                     /locus_tag="ACG8XG_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0774027.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2507..2992)
                     /locus_tag="ACG8XG_002129"
     CDS             complement(2507..2992)
                     /locus_tag="ACG8XG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0774028.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3082..3427
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3472..4113
                     /locus_tag="ACG8XG_002130"
     CDS             3472..4113
                     /locus_tag="ACG8XG_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0774029.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4132..4767
                     /locus_tag="ACG8XG_002131"
     CDS             4132..4767
                     /locus_tag="ACG8XG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774030.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4785..5600
                     /locus_tag="ACG8XG_002132"
     CDS             4785..5600
                     /locus_tag="ACG8XG_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0774031.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5919..7355
                     /locus_tag="ACG8XG_002133"
     CDS             5919..7355
                     /locus_tag="ACG8XG_002133"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0774032.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7544..9991
                     /locus_tag="ACG8XG_002134"
     CDS             7544..9991
                     /locus_tag="ACG8XG_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0774033.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10221..11288
                     /gene="rlmN"
                     /locus_tag="ACG8XG_002135"
     CDS             10221..11288
                     /gene="rlmN"
                     /locus_tag="ACG8XG_002135"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0774034.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11278..11484
                     /locus_tag="ACG8XG_002136"
     CDS             11278..11484
                     /locus_tag="ACG8XG_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774035.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11481..12584)
                     /locus_tag="ACG8XG_002137"
     CDS             complement(11481..12584)
                     /locus_tag="ACG8XG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0774036.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13136..13684
                     /gene="aac(6')"
                     /locus_tag="ACG8XG_002138"
     CDS             13136..13684
                     /gene="aac(6')"
                     /locus_tag="ACG8XG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0774037.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4729 a         2298 c         2926 g         3857 t
ORIGIN      
        1 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
       61 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
      121 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
      181 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
      241 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
      301 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
      361 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
      421 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
      481 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
      541 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
      601 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
      661 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
      721 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
      781 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
      841 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
      901 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
      961 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
     1021 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
     1081 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
     1141 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
     1201 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
     1261 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
     1321 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
     1381 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
     1441 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
     1501 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
     1561 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
     1621 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
     1681 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
     1741 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
     1801 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
     1861 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
     1921 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
     1981 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
     2041 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
     2101 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
     2161 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
     2221 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
     2281 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
     2341 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
     2401 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
     2461 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
     2521 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
     2581 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
     2641 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
     2701 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
     2761 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
     2821 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
     2881 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
     2941 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
     3001 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
     3061 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
     3121 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
     3181 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
     3241 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
     3301 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
     3361 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
     3421 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
     3481 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
     3541 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
     3601 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
     3661 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
     3721 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
     3781 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
     3841 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
     3901 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
     3961 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
     4021 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cgtaatgaaa
     4081 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
     4141 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
     4201 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
     4261 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
     4321 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
     4381 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
     4441 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
     4501 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
     4561 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
     4621 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
     4681 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
     4741 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
     4801 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
     4861 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
     4921 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
     4981 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
     5041 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
     5101 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
     5161 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
     5221 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
     5281 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
     5341 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
     5401 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
     5461 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
     5521 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
     5581 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
     5641 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
     5701 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
     5761 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
     5821 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
     5881 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
     5941 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
     6001 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
     6061 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
     6121 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
     6181 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
     6241 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
     6301 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
     6361 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
     6421 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
     6481 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
     6541 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
     6601 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
     6661 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
     6721 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
     6781 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
     6841 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
     6901 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
     6961 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
     7021 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
     7081 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
     7141 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
     7201 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
     7261 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
     7321 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
     7381 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
     7441 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
     7501 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
     7561 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
     7621 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
     7681 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
     7741 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
     7801 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
     7861 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
     7921 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
     7981 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
     8041 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
     8101 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
     8161 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
     8221 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
     8281 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
     8341 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
     8401 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
     8461 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
     8521 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
     8581 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
     8641 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
     8701 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
     8761 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
     8821 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
     8881 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
     8941 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
     9001 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
     9061 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
     9121 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
     9181 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
     9241 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
     9301 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
     9361 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
     9421 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
     9481 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
     9541 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
     9601 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
     9661 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
     9721 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
     9781 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
     9841 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
     9901 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
     9961 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
    10021 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
    10081 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
    10141 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
    10201 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
    10261 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
    10321 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
    10381 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
    10441 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
    10501 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
    10561 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
    10621 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
    10681 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
    10741 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
    10801 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
    10861 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
    10921 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
    10981 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
    11041 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
    11101 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
    11161 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
    11221 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
    11281 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
    11341 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
    11401 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
    11461 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
    11521 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
    11581 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
    11641 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
    11701 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
    11761 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
    11821 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
    11881 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
    11941 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
    12001 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
    12061 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
    12121 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
    12181 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
    12241 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    12301 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    12361 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    12421 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    12481 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    12541 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    12601 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    12661 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    12721 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    12781 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    12841 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    12901 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    12961 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt attttataag
    13021 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    13081 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    13141 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    13201 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    13261 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    13321 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    13381 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    13441 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    13501 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    13561 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    13621 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    13681 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    13741 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    13801 caccgtgtac
//
LOCUS       DAZKSR010000078        13732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000078 DAZKSR010000000
VERSION     DAZKSR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.078"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG8XG_002139"
     CDS             complement(143..1345)
                     /locus_tag="ACG8XG_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774038.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG8XG_002140"
     CDS             complement(1348..1542)
                     /locus_tag="ACG8XG_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0774039.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG8XG_002141"
     CDS             complement(1589..2437)
                     /locus_tag="ACG8XG_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFQ0774040.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG8XG_002142"
     CDS             complement(2642..2992)
                     /locus_tag="ACG8XG_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774041.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG8XG_002143"
     CDS             3170..4033
                     /locus_tag="ACG8XG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0774042.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG8XG_002144"
     CDS             4657..5649
                     /locus_tag="ACG8XG_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0774043.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG8XG_002145"
     CDS             5665..6549
                     /locus_tag="ACG8XG_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0774044.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG8XG_002146"
     CDS             6598..8205
                     /locus_tag="ACG8XG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0774045.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG8XG_002147"
     CDS             8206..9690
                     /locus_tag="ACG8XG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0774046.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG8XG_002148"
                     /pseudo
     CDS             9867..10859
                     /locus_tag="ACG8XG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            10911..11780
                     /locus_tag="ACG8XG_002149"
     CDS             10911..11780
                     /locus_tag="ACG8XG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0774047.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8XG_002150"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG8XG_002150"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0774048.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGAEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg ctaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc tgcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZKSR010000079        13698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000079 DAZKSR010000000
VERSION     DAZKSR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.079"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1528
                     /locus_tag="ACG8XG_002151"
     CDS             101..1528
                     /locus_tag="ACG8XG_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774049.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1620..1985)
                     /locus_tag="ACG8XG_002152"
     CDS             complement(1620..1985)
                     /locus_tag="ACG8XG_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0774050.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1982..2347)
                     /locus_tag="ACG8XG_002153"
     CDS             complement(1982..2347)
                     /locus_tag="ACG8XG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0774051.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2539..2787)
                     /locus_tag="ACG8XG_002154"
     CDS             complement(2539..2787)
                     /locus_tag="ACG8XG_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774052.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2786..2914
                     /locus_tag="ACG8XG_002155"
     CDS             2786..2914
                     /locus_tag="ACG8XG_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0774053.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2971..4665
                     /locus_tag="ACG8XG_002156"
     CDS             2971..4665
                     /locus_tag="ACG8XG_002156"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0774054.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4765..5061
                     /locus_tag="ACG8XG_002157"
     CDS             4765..5061
                     /locus_tag="ACG8XG_002157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774055.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5439..6572
                     /locus_tag="ACG8XG_002158"
     CDS             5439..6572
                     /locus_tag="ACG8XG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0774056.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6603..6923
                     /locus_tag="ACG8XG_002159"
     CDS             6603..6923
                     /locus_tag="ACG8XG_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0774057.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6952..7416)
                     /locus_tag="ACG8XG_002160"
     CDS             complement(6952..7416)
                     /locus_tag="ACG8XG_002160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774058.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7547..8800)
                     /locus_tag="ACG8XG_002161"
     CDS             complement(7547..8800)
                     /locus_tag="ACG8XG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0774059.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8829..9146)
                     /locus_tag="ACG8XG_002162"
     CDS             complement(8829..9146)
                     /locus_tag="ACG8XG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0774060.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9172..9492)
                     /locus_tag="ACG8XG_002163"
     CDS             complement(9172..9492)
                     /locus_tag="ACG8XG_002163"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0774061.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9956..11371)
                     /locus_tag="ACG8XG_002164"
     CDS             complement(9956..11371)
                     /locus_tag="ACG8XG_002164"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0774062.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11388..12704)
                     /locus_tag="ACG8XG_002165"
     CDS             complement(11388..12704)
                     /locus_tag="ACG8XG_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0774063.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12854..13552)
                     /locus_tag="ACG8XG_002166"
     CDS             complement(12854..13552)
                     /locus_tag="ACG8XG_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0774064.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4349 a         2782 c         2357 g         4210 t
ORIGIN      
        1 atttatatgc tttttcagaa attaactgat gatttcaaaa agaaaacatg tcataatatt
       61 tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag aatatattga
      121 gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata aagaagtcaa
      181 tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca atgatatcaa
      241 taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa aggaccgtta
      301 cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat tgtctcaaaa
      361 ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg attatcttca
      421 attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa aacaagtatt
      481 agcttatttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat tcactgaaat
      541 ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta attcttggga
      601 gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag
      661 tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg ctatcagttt
      721 cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa
      781 acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc aaacctatta
      841 taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt atggtttcca
      901 ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta ttgacaatac
      961 tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag aattattcgg
     1021 aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg ataactatca
     1081 attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt tatataaaga
     1141 agtccagaca attttttcca gctggtccag ccagttacct tatgatctac ggttgaatcc
     1201 taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga caaaagaaca
     1261 tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc gggaagcctt
     1321 ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa gtatatctga
     1381 tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta cattatatac
     1441 accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga tcgatcgagc
     1501 tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt cacagcctct
     1561 tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg ctcacaacct
     1621 cacttatttt ggacatctac gggtccgatc tctttattta aaaaataaga aatgatggtt
     1681 cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt gataatcgat
     1741 tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc tcccaacatc
     1801 tttttaatag cattgttctc gctgactcgg tcgctttgca cttttgtatg gacaaataaa
     1861 tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca gatagcaaag
     1921 atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat caactctgtc
     1981 gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga ttcaagaaat
     2041 aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct gtccaagtcg
     2101 gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt aacaaaatat
     2161 aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca tagtttttct
     2221 tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca attaagatac
     2281 cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt aaatttgagc
     2341 gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt gtaaaagaaa
     2401 gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt aaatttttct
     2461 ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc aaaacaacgt
     2521 agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact gtctcggtgc
     2581 tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc ttttcttctt
     2641 acctacccta tttttattta caggaaaatc agacacttct tcttttttgt caaaatttcc
     2701 tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct tggatataga
     2761 aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg gcatcggttt
     2821 aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat tcgaagatga
     2881 cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga atcactacgg
     2941 acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat aatgattggt
     3001 gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa atggaaaggg
     3061 gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc accgaccgac
     3121 actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa aggctatatt
     3181 gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga tctgttaagt
     3241 cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag agattttgat
     3301 gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg aatcattgat
     3361 ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact gattgctatg
     3421 cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt caaagatgat
     3481 ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc tttcagaaca
     3541 cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga attcactcaa
     3601 atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag tattatgctt
     3661 cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact
     3721 gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt
     3781 accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac agcagttatt
     3841 ttcactaatg ggtccattac agattcagct actttaggag attacgaaac accagctgta
     3901 gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga acgtttctac
     3961 aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg ggtcagcttt
     4021 accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac gactcaagaa
     4081 ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt aggccaaaaa
     4141 gatgtcaata tgtctatcgt cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat
     4201 gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt tgtggataaa
     4261 cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca actttcaaaa
     4321 gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga cagcgtaata
     4381 aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg tgccaaaagc
     4441 gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg agaatttgtc
     4501 gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg agaaatcgct
     4561 gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa
     4621 gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa
     4681 aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct tatgtttttg
     4741 atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt
     4801 gaaaaagctg ccctgcatca agtaataaga acacccaagc taaaactgct tcttatgttt
     4861 ttgacttggt tggacttatt accgcagatg caagcttttg aacaccattt accaagaggt
     4921 tatgaaaaag ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg
     4981 tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
     5041 ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc
     5101 acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt
     5161 tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca
     5221 atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg
     5281 aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa
     5341 gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata
     5401 atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag
     5461 gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg
     5521 caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg
     5581 gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag
     5641 aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata
     5701 ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa
     5761 atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg
     5821 gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag
     5881 ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt
     5941 ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc
     6001 cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag
     6061 tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt
     6121 tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag
     6181 atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa
     6241 atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg caagctgtca
     6301 aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc
     6361 tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc
     6421 taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca caaatcatta
     6481 ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag
     6541 ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct
     6601 ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg
     6661 aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg
     6721 ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa
     6781 tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc
     6841 ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa
     6901 caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt ttcaccctat
     6961 cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa
     7021 tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta
     7081 cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt
     7141 acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag ggcggttatg
     7201 gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac
     7261 ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac
     7321 aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg
     7381 gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg actaaaataa
     7441 aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt
     7501 aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag
     7561 cttcttttct tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag
     7621 aaagaaccaa attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac
     7681 cgccaataat tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg
     7741 tagaagtcgc aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga
     7801 acatgatcgg gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga
     7861 cacctggcgc aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta
     7921 aataaatagc taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt
     7981 caaagaaagg tttgttcacg ataaacggtg cgctatgccc agctgcgatt gcattaacgt
     8041 ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt
     8101 gtaaaccaaa gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag
     8161 agtttgcaag tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat
     8221 caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg
     8281 ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga
     8341 tgatcaattt ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa
     8401 taatagcaat aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag
     8461 agcctaaaga aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat
     8521 aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag
     8581 ccccgaatcc tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg
     8641 gaatcggtaa gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca
     8701 ccataaatga gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag
     8761 gtataaaatg tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt
     8821 actgtagatt acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta
     8881 aaagctatag atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg
     8941 tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa
     9001 gattcctctg catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca
     9061 gcaatcgctt ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg
     9121 gcttctagat ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt
     9181 tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat
     9241 attgattaca tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat
     9301 aaagcgtact tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc
     9361 cgcatctgaa gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt
     9421 ttgcattttt gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt
     9481 tttcttttcc attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc
     9541 gtgccaactt taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag
     9601 ttgcatacac actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt
     9661 ttttctttta acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa
     9721 taaacggtat tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
     9781 atgttgtttt tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct
     9841 caataaacgg tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac
     9901 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
     9961 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
    10021 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
    10081 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
    10141 cgcaatttgc gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt
    10201 cgatacggta atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc
    10261 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
    10321 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
    10381 tcccaggtaa atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc
    10441 attgataacg aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta
    10501 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
    10561 tttgacacaa ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata
    10621 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc
    10681 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
    10741 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
    10801 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
    10861 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa
    10921 tttgagcata cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt
    10981 ccaaaagaaa agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt
    11041 cgatcaaaga gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt
    11101 tctcataatc agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga
    11161 tggatttcat cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg
    11221 ggccataccc attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat
    11281 cctgtccttc tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa
    11341 gaataaaatt ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca
    11401 ttttccacac gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata
    11461 caaacaagtt gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg
    11521 atcggcggca ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt
    11581 gcaaaataac caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt
    11641 ggattcagca tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga
    11701 gcaagggata acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca
    11761 atcagtaaac cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc
    11821 atagtaataa tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt
    11881 gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc
    11941 acccaaaaga cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca
    12001 ccttctaatg ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt
    12061 tctttgatga taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa
    12121 gcagaaaaac tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc
    12181 ttctgtttcc tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa
    12241 aataaccctt tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga
    12301 ttttcattca ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg
    12361 ataccaggga atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa
    12421 acagcataga tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt
    12481 aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc
    12541 attgctgaaa acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat
    12601 gcgtttttta tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa
    12661 acattggcta atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct
    12721 tgacgtcatc tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt
    12781 actcctcttt tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac
    12841 atcagatgaa ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat
    12901 ttattgccat atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg
    12961 cctgaacgat ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat
    13021 ttttattaaa caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt
    13081 ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg
    13141 tgccttctcg aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat
    13201 tcttcgttgc atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt
    13261 caaataagtc tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat
    13321 tggcaatccc tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat
    13381 tcctggaagc tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat
    13441 cggacaattc atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat
    13501 atctaaggat cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata
    13561 cctccttttc atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg
    13621 attttttgta tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta
    13681 ccataaaaaa gactagga
//
LOCUS       DAZKSR010000080        13695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000080 DAZKSR010000000
VERSION     DAZKSR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.080"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1610)
                     /locus_tag="ACG8XG_002167"
     CDS             complement(45..1610)
                     /locus_tag="ACG8XG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774065.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(1603..3054)
                     /locus_tag="ACG8XG_002168"
     CDS             complement(1603..3054)
                     /locus_tag="ACG8XG_002168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0774066.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(3068..4084)
                     /locus_tag="ACG8XG_002169"
     CDS             complement(3068..4084)
                     /locus_tag="ACG8XG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0774067.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4304..5392
                     /gene="chvE"
                     /locus_tag="ACG8XG_002170"
     CDS             4304..5392
                     /gene="chvE"
                     /locus_tag="ACG8XG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0774068.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5446..6972
                     /gene="mmsA"
                     /locus_tag="ACG8XG_002171"
     CDS             5446..6972
                     /gene="mmsA"
                     /locus_tag="ACG8XG_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0774069.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6974..8179
                     /gene="mmsB"
                     /locus_tag="ACG8XG_002172"
     CDS             6974..8179
                     /gene="mmsB"
                     /locus_tag="ACG8XG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0774070.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8436..9413
                     /gene="guaC"
                     /locus_tag="ACG8XG_002173"
     CDS             8436..9413
                     /gene="guaC"
                     /locus_tag="ACG8XG_002173"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0774071.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9677..11101
                     /locus_tag="ACG8XG_002174"
     CDS             9677..11101
                     /locus_tag="ACG8XG_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0774072.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(11143..11400)
                     /locus_tag="ACG8XG_002175"
     CDS             complement(11143..11400)
                     /locus_tag="ACG8XG_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774073.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            11562..12671
                     /gene="pdhA"
                     /locus_tag="ACG8XG_002176"
     CDS             11562..12671
                     /gene="pdhA"
                     /locus_tag="ACG8XG_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0774074.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            12674..13651
                     /locus_tag="ACG8XG_002177"
     CDS             12674..13651
                     /locus_tag="ACG8XG_002177"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0774075.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         4368 a         2345 c         2962 g         4020 t
ORIGIN      
        1 ttttattgca aatggaaaga gtatcattgt acggatttcg tttattagtt atttttgttt
       61 ttaaggaaaa gctgtgtccg atattctttt ggagaaatcc cttgtttttt cttaaataaa
      121 taactgaaat agttagggtc attataacca atttcaaaag caatatctga aatacgatag
      181 tctgattgct gcaataattt ttttgctaat tcgattcttt gttctgtcag ataatcgatg
      241 aatgtttggt taaacgattg agaaaaaatc gtactgaaat gagagggact taatgcaact
      301 tgttgagcga cccaagttaa ggacatatct ggatcggtaa aattttcttt gataaaatcc
      361 aatgcatggt tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg cagagcagtt
      421 aagaaagaaa tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc gctgattgct
      481 aagcgttgac tggtggatgt gcaatgaacc atttcttgcg ggattttctt ttctttcagg
      541 cagacatgct ggagtagctt agtcaagaca aataaacgat ataatcgtgt tcgagttggt
      601 gtgccttgtg gtttcgtcaa gtgttcgatg tacttagata cttcgttttt cgataatgaa
      661 gacaaatcaa gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt cgcttcttga
      721 atcaaatcct catagctgat aatttgttct ttaagggaac tggcggtttc aagtatcatc
      781 tgcgtgatct caaaagatgc aggtaattcg ctgattcttt ctgcgacagg accaatggcg
      841 acagaaaaat tatttttttg tcccttttct aattcatgga ccaacgtttg ggctacctga
      901 taactggtct cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt gatgaatcga
      961 gaggaaacag cagagaagat cacttgttga ttctccctaa ataaaaactt caaataatca
     1021 ctgagatgag cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa taatacggtc
     1081 atttttttcc ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt ttcactgatc
     1141 gtaagtgagc ctttgaacaa accgttcaag aaatgatttt ttttcaattc gaaaacaaga
     1201 tcagaagaag tgtgcgtttc tttttgctgc aaatctaaag cagttactgc tttttctatt
     1261 gcttgtttca attcgctact tttaatcggt ttaagcagat aatcatctgc ctgtacctgg
     1321 attgctcctt tcatataatc gaaatcgtcg aatccactaa taaatatgag acgagtccaa
     1381 ggaagtagtt tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg catttggata
     1441 tcagtcagaa tgatgtcggg ctgtaagtcg ataattgaag acagtgccat ctcaccatct
     1501 ccagcttcgc cgacataagt gattggataa taagaaccaa ggacagttat ttgttcccgc
     1561 aagttattgc gaatgaggta ttcgtcttcg atgataaaaa ctttatacat gtttgtttcc
     1621 tccaattgtt ttgggagata gagggatcat cagtgtgaca gttgttcctt ctcggtattt
     1681 gctgtcaatc gtgataggtt tttgatcatt tccgtaataa agaaggagac gcttgtttac
     1741 gttgtaaagt ccgtagccga tactgaaatc tgaatcgatc cctttggcta actctttacg
     1801 tatttctgcc agtttttctg cagggatgcc aatcccgttg tctataactt tgaattggat
     1861 catctcatca attttgcatg tggtaatacg aataaatcct ttgtgacgga cgtacttcgt
     1921 tccatggtat acagcatttt caatcaatgg ctgcaagatc atttttaaaa tagaaacagg
     1981 aaaaagtgtt tctggtatat cgatcttata gtcaagcata tggccgtatc ttatttttaa
     2041 gatgactaga taatcattga tatggctaat ttctttttca agaggaatcc agtcttgtcc
     2101 tttgcttaaa gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca gagcttcttc
     2161 aaagcagtct tgttcgatca aagaaataat ggcatccaaa ctgttataaa taaaatgggg
     2221 agtaatctga gcttgcaata cacgaacttc actttgtgct aaatggtatt gtttcaaagc
     2281 gttctcccgt aaagactggt ttagatcttc cgccatttta ttcatgctag cggtaagtgt
     2341 ttgtaattcc agtgtttccg gcagggttaa acgaaagcca aggttccctg aagaaagttg
     2401 ttctgacatc tcgatcagct gattgatcgg ttcttggacc tttctctcta aaaagcgacg
     2461 attctttttc gcatagaaaa aaatggtact aagaactcca agttccacta ttgttaatag
     2521 aatgagcgag cggacaagtg tcgtattttt tttgcttgct aagtcgatct ccacacgaac
     2581 aaaatcttgt aagatatctg acagcagttg agtgacagaa tctacctgat tgatgatctc
     2641 ttcattggta tccataaaat cttttgccaa gatattattg atcagctcgt tttgataatc
     2701 ttccagtgta tcgatcgtac gtagcgatac atctaacgta ctaatttctt taagtgtatt
     2761 tgtattattt ttgatctctc gaatatcatt cctaagttct tctaggatat tttcctcatt
     2821 gaactcatct acggttactt ggccagaaat caagtcccac atactttcca aaactttttg
     2881 atcaaccttc aaagcaattg catctgcttt ttggatattt gcgatcgtct gttgataaat
     2941 caaaagctgt tgcgaatata acagtatgct cagtagcaaa gggataacag caaatagaag
     3001 catggctttg tttgcttggg caaaaatctg ttttatgctt aaattttttg tcatgatact
     3061 tctccagtca ataatttctg gttgggatgt tagctatatt ctgctgggaa aagacagtct
     3121 cggaaataaa aatatcttta ggtaatgttt ttcctgtttg aatatttcca aaataacgag
     3181 taacggtatt ggctacatac cagccgacat caggtttaca ctcgactaca caattgacga
     3241 ttcctgagcg tagcttctcg atcatatctt cttgtccatc aatagttaca atgatcaaat
     3301 cttctccagg tttgattgat gtttctttta tcgcagctaa agcaccgtga gtcatttcat
     3361 cactatggct gtataataca tcgatttcgg ataaatcatt tgtttctaaa tatcgtttca
     3421 tctggtcttt tcctttcatc cgaataaaat caccgacaat tcggtgaaca atagtcatcg
     3481 gaggtttatg gtcgatggtt ccatcttttt ttagggttgg tttacgattg attgattcca
     3541 aaaatccttc ggagcgtagc ttggtcggtg tggagttctc tgaaccagca agttctagga
     3601 ttctgatttc ctgtttcttt gaatttgtga agtgattgct gacaaataaa cccgcccgat
     3661 ttccttctgc tttgaaactg gggccaatgt gggtgacgaa aagctcggga tcattcgttt
     3721 gaatatgtct gtcgacaagt atgacaggga tccctgctcg ctttgcttct tttaatacag
     3781 agtcccaccc ggattcttgc aaaggagtaa aaatgatcat atccacttta taggcaatga
     3841 acgtccgtat atcttggatt tgccgttcct gattcatgta gccatttcgg tagagaactt
     3901 ggtaatcttc tttttcaagc gcagtgataa ttgattcagt atgctttttc cgccagctac
     3961 tctccgttcc tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc tttgtattag
     4021 gtgtacaccc catcatttgg taagcgatta caacaagggc taaaaaaaat aaaacacgtt
     4081 tcactttaaa tcactccatc tggatttgtt agtactatct tatcagataa aagggaaaat
     4141 caaagtcaaa aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc caagacaaaa
     4201 aaagtacacc taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta acgctttctt
     4261 tattagctat actcggtgta tcaattgaaa tgggggataa aaaatgaaaa acaaatttag
     4321 aaagattgtt gggactatcg ctattttgac cgcaagcatt ctattaagtg cgtgtggcaa
     4381 ttcaggatct gcagatgata gtacggggta tgtaggaatc gctatgccga ccaaatcagc
     4441 agaaagatgg atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac tgggttataa
     4501 aacagatctt caatatggag aagataaagt agaaaatcaa gtagcacaaa tagaaaatat
     4561 gattacaaaa ggggtagata cacttgtgat tgcttctatc gatggttcgg ctttaactga
     4621 tgtgttagct aaagcaaaag aagcagatat caaggtgatc gcctatgatc gtttactgat
     4681 gaattcagaa aatgtcgatt attacgcgac ttttgataat tttggagtag gtgtatcgca
     4741 agcagcttat attgaagagc acttaggatt gaaagaagga aaaggaccat tcacgattga
     4801 attatttggt ggctcgcctg acgacaacaa cgcactaatc aattacaacg gagtgatgtc
     4861 tgttttacaa ccatatatgg acaacggtca gttggtcgtg ccatctggac agacgagttt
     4921 cagtcaaatc gctacgcttc gctgggatgg ttctacggcg caagcaagga tggataatct
     4981 gttaagcgca aattatactg accaaacatt ggatgctgtt ttgtcgccat atgatccaat
     5041 aagtttagga atcatttcgt cattaaaagg cgtggggtat gggagtgaaa gtaaaccttt
     5101 acctgtgatt actggacaag atgcgacagt tgctggcgtt aaatcgatca ttgccgggga
     5161 acaaacacaa acgatcttta aagacacacg tatcttagca aaaaatacga ttgaaatgat
     5221 caaagcaatc agtgatggag aagaggttcc ggtcaatgat actgaaactt atgataatgg
     5281 ggtgaaaact gtccctacgt atttagcaaa tacagtatct gtcgataaag ataattatca
     5341 agcagagctg atcgacaccg attattataa agaatccgat ctgaaaaatt aagaggaacc
     5401 aaacaaggaa ctaaagagat ctaagtcaga gagaggataa gaattatggc ggattgtatt
     5461 ttaaaaatgg cagatatcac caaaaaattt tctggaatcc gagcgttaga ttgcgtgaat
     5521 ctgatgattg aacgtgggga aatccatgca ttgtgcggag aaaacggagc gggaaaatca
     5581 acattgatga atgtcttgag cggcttgtat ccatatggct cctatgacgg ggaaatcttt
     5641 tatgatggaa agctgtgtca atttaaagat ttgaaagata gtgaagaaaa aggcatcgtc
     5701 atcattcatc aagaattagc actaagtcct tatctttcaa taaaagagaa tatttttctc
     5761 ggaaatgaac aagcagaaag aggcgtgatt gattgggaac tgacagaaaa gaaaacatat
     5821 gaactgctga ataaagtcgg tttgtctatc gacccgaatc ttctagtatc ccagattggc
     5881 gtagggcaac agcaactagt tgaaatagct aaagcattat caaaatccgt ccgtctgttg
     5941 attttggatg agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt gctgagattg
     6001 atccgagagt tgaaagagca aggtgtaacc tccatcatta tttcacataa gctgaatgaa
     6061 atcgtggata tcgcagatcg aatcaccatt ttacgggatg gacagacgat tgagacacta
     6121 gaaaatgaaa tgatcagcga agagcatatt attcgcggaa tggtgggcag agatttagtt
     6181 gatcgttatc ctgaaagaaa accggatatt ggagaagttt attttaaagt agaagactgg
     6241 acggtctatc accctcttga tcacgagcga atcataaacg accacatcaa cttgcatttg
     6301 aaaaaaggag agattgtagg aatagctgga ctgatgggag cgggcagaac tgaattcgct
     6361 atgagtctat ttggacattc ttatggaagc aagatatccg gacgtatatt aaaggaaggg
     6421 gcagagatat cagttaagac cgttccttca gccattgcaa acggactcgc ttatgtctcg
     6481 gaggaccgaa aatctttagg actgaatctg ctgatggata ttcgggaaaa cgtgacgctg
     6541 agcagtttgg ataaaataag tcaaaaagga gtattagata aagaaaaaga agtaactgag
     6601 gcagaaaatt tccggaaaaa gatgcgtata aaaacaaact ccatccatca aattgtcagt
     6661 agtcttagtg gtgggaatca acaaaaagtc gttttatcca agtggctgat gacacaaccc
     6721 gatattcttt ttttggatga gccaacccgt gggatagatg ttggggcaaa atatgagatc
     6781 tacacgatta tcgaagaaat ggcagcaaat ggcaaagctg tctgtcttat ctcttctgaa
     6841 cttcctgaaa tattagggat gtgtgatcga atctatacga tgaacgaagg acgtattaca
     6901 ggagaagtct cgcgagaaga tgcagatcag gaagtcttga tgaaattaat gacgaaagaa
     6961 gaggtgggtt aagttggaac aggcaaaaaa tcttacggat aaaaaagaga aaaaactaaa
     7021 tttgaaaagt agagcagctg atctttttgg tcgctacagc atggtgatca ttttagcggg
     7081 actacttatt gcgtttcaat tgatgacgga tggtattttc tggcgtccat tgaatataac
     7141 taaccttgtt ctgcaaaata gccatatatt agtactagct gcagggatgt tattagttgt
     7201 gctcttaggt catgtggatt tgtcggtagg gtcagtgatg gcgtttgttg gtgcaattgc
     7261 gggcatgatg atggtcaatc gccaaatgaa cccgtggatg acagttatcg catgtcttgc
     7321 agtaggcggg ctgatcggcg catggcaagg tttttgggta gcttatgcag ggataccggc
     7381 gtttatcgtg actttagcag gattgctgat gtttcgtgga ctgacacaag ttgttctagg
     7441 aggccaatct ctagcacctt ttccagctgt tttccaaaaa atgtctacgg gttatttacc
     7501 agacttaagc gaaggaagca gccttcactt cttgacattg attttaggaa tgatcatcac
     7561 gatcgtgtta gttgttggtc aatggcgaac aagaaatcgt cggaaaaatc atttatttga
     7621 agtggaatct ccaacagctt tcttggtaaa ggcggtattt acagcagtcg ttgtactagg
     7681 aataacttat atctttgcct cttatcaagg gtatccgatt attttgatca ttcttggttt
     7741 gatcgttgca gcatatgggt ttttgacaaa taaaacagta gcaggtcgac agatttatgc
     7801 gacgggcgga aatcgaaaag cggctgaact atcagggatc aaaacaaagc gaatcacgtt
     7861 ctgggtattc gtcaacatgg gagtcatggc agctttagct ggattagttt tagctgctcg
     7921 attgaatgcg gcaacacctc aagcaggtac atcgatggaa ctggatgcaa tggcggcagt
     7981 atattttggt ggtgcttcga cttccggcgg cattggaaca atcatgggtg cgattgtcgg
     8041 tggcttggtc atgggtgtat tgaacaatgg catgtcgatt ttaggcgtcg gggtagactg
     8101 gcagcaggca atcaaaggat tgatcttgct tttagccgtt gtgctagact tgtataataa
     8161 gaaaaaacga acaacgtagg agaacgccgg ttattcagaa aaaagactgt gacaagacat
     8221 ttgtcacagt ctttttttcg ctaaaagcga acattcattg tttcgtttgt atagactaaa
     8281 aataatatat gtttttttac cattcgtata aattctcttt ttgctcttga tttttttgct
     8341 taattgagtt aaattacata gggcgctaaa aaaagccttg attaaattaa taatgttcgt
     8401 gttttgctgg atgtgaacat caggaggata atgtaatgaa agtatttgat tacgaagata
     8461 ttcaattaat tcctaataaa tgtatcgtaa atagccgttc tgaatgtgat acaacggtca
     8521 cattaggtaa acatacattc aaaatgccag tagtgcctgc caacatgcag acgattattg
     8581 atgaaacaat cgcagaattc ttagcggaaa atggttattt ctatatcatg catcggtttg
     8641 atgaagcagc tcgaattccg tttatcaaaa aaatgaaaaa gcgtggattg attacctcga
     8701 tcagtgttgg cgtcaagaaa gaggaatatt cctttataga gaagttggca gaagaatcgc
     8761 taaatcctga ttatatcact atcgacatcg cgcatggaca tgctaactca gttattgata
     8821 tgattcagca tattaaaaaa tatttgcctg aaacttttgt gattgccggt aatgttggta
     8881 caccggaagc cgtccgtgaa ttggaaaatg cgggcgcaga tgcaacaaaa gtcgggattg
     8941 gtccaggaaa agtatgtatt acaaaaatca agacaggctt tgggactgga ggctggcaac
     9001 tggcagctct tagatggtgt gcgaaagcag ctcgcaagcc gatcatcgct gacggtggga
     9061 tcagaacaca tggcgatatt gcaaaatctg tccgttttgg tgcgacaatg gtcatgattg
     9121 gttcattgtt tgccggtcac gaggaatctc ctggcgaaac aaaagtcgaa aatggaatcg
     9181 tgtataaaga atactttggt tctgcttcag aattccaaaa aggcgaaaaa cgaaatgttg
     9241 aaggaaagaa aatctggatc caacacaaag gatctttgaa agatacacta gtggaaatgc
     9301 aacaagacct tcaatcatct atttcttatg ctggaggaag agatctggaa gccattcgaa
     9361 aagttgacta tgtcatcgtg aaaaattcga tttttaatgg agacacaatc taaaaaataa
     9421 ataaaatatt aggatgttgt gaaaaagttg tcctgcatta agtaataaga acagccaaac
     9481 aaaaatagtt ctttatatgt tttgtttggc tggaattatt atcgcagata caagcttttg
     9541 aacatcatta ttcagaataa gggactgtga taagattttt gtcacagtcc cttatgtttt
     9601 gcaaaaaaag tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa aaatagacaa
     9661 tagaaaagag aagcttatgt gggaaaaagt agagaaaaaa caaggaccga tttatcgtca
     9721 aattatggaa cagattatga gagaaatcga aaacggaagg atcaatcctg gaagtcaact
     9781 tgaatcagaa cgaaaactag ggcaactttt tggtgtgaat cgttccaccg ttgtccatgc
     9841 attggaagag cttagggagc ttggggtact aacgagcaaa cgtggcagcg gatggtttgt
     9901 taatcagact gaatggggga aattcgctgt tccaagaatc gattggcggc aaatgatctc
     9961 tcctcgttat gagcagacgg actggtatga gcaaaaaata agagaagcaa agcagatcca
    10021 aaaaacatcg tttcttgatt tatacgctag cgagatgcca gatgagcttt tgccgaattt
    10081 tgagtttcct tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa tggatctttt
    10141 aggctatact ccgttacgta acaaaatcaa acggtatttg gaaagtaaat ttgaattggc
    10201 attttccccc aaccagcttc ttgtgactgg tggcggtcag caagcaatct ttttgatttt
    10261 acaaacgatt ctttcagctg gagaggggat agctgtagag tcaccttctt ttttttaccg
    10321 attacctctt ttcaaagctt caggaacgcg tttatttggg ataccgatgg atgaagaagg
    10381 gatcgactta gaaaaactga aagaatcgat ccataaaaat aaattgaaag cagtgcttat
    10441 caacccgaat tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac gagaaaagct
    10501 agtagaattg tgtcgacagt atcggcttcc aattattgaa gacgatgtat ttacggatct
    10561 ttcatttcta aaccaagaag aagtccttcc tatacgaacg attgatccgg ataatgtgtt
    10621 atacgtcggt tcgttatcta gggttttagg aaaaacaacg aaaatcggtt gggtgatagg
    10681 acctgagact tttatctcgc aactagctca agcgcaagaa atgatggagt tttcgatgaa
    10741 tatccttaca caaatcacag tggctaatgt atttaatgaa gcaattgatt tgaaaatgca
    10801 agatgtaaga aaaaagttga aagaaaaaag ccgactgata agcgcttggg gagattcaca
    10861 atcgtttttc aagcttcatt caattttagg tggttactat gcctggatta cttgggatgg
    10921 gaaaaaacta gaccccgaac tagcagaaaa agtagtagat atgggtcttg gaattgcacc
    10981 aagtctactt tttgggcaag atataaatgg aatcagaatc aattacagta ggctggatga
    11041 aaaccaaata ccgttgtttt attcaaagat gaaaattcta gaaaagtggt taacagaata
    11101 atttcacaaa gtaaatagat taaagaatat aacagcttgg atttaaattt caagctgttt
    11161 cttttttttc ggaaaaagaa tacgatataa agaaactaag aaaaaaccat tatttgtacg
    11221 tccgttcacg atgtgtaaat aaaataaatg tttgcaacaa aactgtataa caccaacgtt
    11281 tttaaataga aacagatggt tgtaagtttg attagaaaat agaaaaaatg cagatgaaat
    11341 ataaaacgct ttccttattg tgattttatt cactaatgct gttaatatgc ggattggcat
    11401 aatttccttt catcgtgaaa atttgtgctt ttagcttgta tttctcataa atccatgata
    11461 atttattaat gaagacggtt aaatagtgat acagttttat tcactgtttc aatcgcagaa
    11521 tgaagagatt ttcttataga tgagagagga atgtgtgaca aatggcaaac aaaaaagcac
    11581 cgatcgactt ccaagcactg ctagatgaag tcaatgcaga tttcccagta tatcaagcac
    11641 ttgatcaaga tggaaacgta gttaacaaag atttagtacc tgatttatcc gatgatgaac
    11701 tcgttgaatt aatgactcat atggtatggt ctcgtgtatt agaccaacgt tcaacagcgc
    11761 tgaaccgcca aggacgccta ggcttttttg caccaactgc tggacaagag gcaagtcagc
    11821 tggctagtgc ttacgcattt gataaagaag atgttcttct tccaggttat cgggatgtac
    11881 ctcaactagt aaaacatggt ttacctttat ctcaagcttt tttatggtct cgaggacatg
    11941 ctgcaggtaa ttcttatccg gaagaattga aagctctacc accacaaatt atcatcggtg
    12001 cacaatatgt acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac aagaaaaatg
    12061 tcgtcttcac ttatactggt gatggcggtt catcacaagg tgatttttac gaagcaatca
    12121 atttcgctgg cgcttaccat gcaaatgccg tattttatat ccaaaacaac gggtatgcga
    12181 tctctacacc tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa gctgtcgcag
    12241 ctgggattcc tggtattcaa gtcgatggaa tggatccgct tgcagtatat actgtatcta
    12301 aaatggcacg ggaatgggcg atcagtggaa atggaccagt cctaatcgaa acactgactt
    12361 atcgttatgg accacataca ctatctggtg acgatccaac ccgctaccgt acaaaagaca
    12421 cagatgacga ttggcagaaa aaagatccat tgatccgctt ccgtaaatat ttgacggaaa
    12481 aaggactttg gtctgaagaa aaagaagaac aagtcatcga agcaactaaa gaagaaatca
    12541 aagcagctat tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat ttcttgaaaa
    12601 acatgttcga agtatctcca caatcaatca aagaacaaat tgcaatttat gaagcgaagg
    12661 agtcgaaata atcatggcac aaaaaacaat gatccaagca atcacagatg ccttagcact
    12721 tgaactagaa aatgacgaga atgtcgttgt ttttggtgaa gatgttggga aaaacggagg
    12781 agttttccgg gcaactgaag gattgcagga aaagttcgga gaagaccgag tattcgatac
    12841 tcctttagct gaatctggaa tcgcaggatt gagtttcggt cttgcgttag aaggatttcg
    12901 tccagttcct gaaatccaat tctttggttt tattttcgaa gcaatggacg aagtagtagc
    12961 tcaaatggct cgtacaagat atcgaatgag cggcactaga aatctaccca tcacaattcg
    13021 ttcgccattt ggtggcggtg ttcatacacc agaattgcat tcggataatt tagaaggttt
    13081 gatcgctcag tctcctggta ttcgagtagt gattccttcc aacccttatg atgcaaaagg
    13141 actattgatt tcttctatta gaagtaacga tccggttgtt ttcctagaac acatgaaact
    13201 atatcgctca ttccgagaag aagtgccgga tgaagcttac gaagttcctt tggataaagc
    13261 agcagttact cgtgagggaa cagatgtatc tattatcaca tacggagcaa tggtgcgtga
    13321 agcaatcaaa gcagcagaca atcttgcaaa agaaaatata tctgttgaaa tcattgatct
    13381 tcgtacagtt gcgccattag atgtagaaac gatcattcaa tccgtggaaa aaactggtcg
    13441 cgtagttgtt gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag tagtttctga
    13501 gatctcagaa cgcgctatcc tttcattaga agccccaatc ggacgagttt ctgcacctga
    13561 tacagtcttc ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa aagatatcga
    13621 agacaaagta aaagaaatcg cggaatttta agaaaagaag ggacgaaaca aagtcatggc
    13681 ttatcaattt aaatt
//
LOCUS       DAZKSR010000081        13679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000081 DAZKSR010000000
VERSION     DAZKSR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.081"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..297
                     /gene="yaaA"
                     /locus_tag="ACG8XG_002178"
     CDS             55..297
                     /gene="yaaA"
                     /locus_tag="ACG8XG_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0774076.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            284..1408
                     /gene="recF"
                     /locus_tag="ACG8XG_002179"
     CDS             284..1408
                     /gene="recF"
                     /locus_tag="ACG8XG_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0774077.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1405..3351
                     /gene="gyrB"
                     /locus_tag="ACG8XG_002180"
     CDS             1405..3351
                     /gene="gyrB"
                     /locus_tag="ACG8XG_002180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0774078.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3371..5842
                     /gene="gyrA"
                     /locus_tag="ACG8XG_002181"
     CDS             3371..5842
                     /gene="gyrA"
                     /locus_tag="ACG8XG_002181"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0774079.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6078..6377
                     /gene="rpsF"
                     /locus_tag="ACG8XG_002182"
     CDS             6078..6377
                     /gene="rpsF"
                     /locus_tag="ACG8XG_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0774080.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6427..6939
                     /gene="ssb"
                     /locus_tag="ACG8XG_002183"
     CDS             6427..6939
                     /gene="ssb"
                     /locus_tag="ACG8XG_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0774081.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6965..7201
                     /gene="rpsR"
                     /locus_tag="ACG8XG_002184"
     CDS             6965..7201
                     /gene="rpsR"
                     /locus_tag="ACG8XG_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0774082.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7344..9317
                     /locus_tag="ACG8XG_002185"
     CDS             7344..9317
                     /locus_tag="ACG8XG_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0774083.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9323..9775
                     /gene="rplI"
                     /locus_tag="ACG8XG_002186"
     CDS             9323..9775
                     /gene="rplI"
                     /locus_tag="ACG8XG_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0774084.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10049..11416
                     /gene="dnaB"
                     /locus_tag="ACG8XG_002187"
     CDS             10049..11416
                     /gene="dnaB"
                     /locus_tag="ACG8XG_002187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0774085.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12196..13488
                     /locus_tag="ACG8XG_002188"
     CDS             12196..13488
                     /locus_tag="ACG8XG_002188"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0774086.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4557 a         2472 c         3014 g         3636 t
ORIGIN      
        1 gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg ggcattgaaa
       61 caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa agaagtcaca
      121 gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt gttagtcgat
      181 ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt tgaagttcca
      241 gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct
      301 gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca agaacctcgt
      361 catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt
      421 agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct ggactgacga
      481 tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat tagaaatctt
      541 attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacaaaaaa agttaagtac
      601 ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt tagtaaaagg
      661 cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca atccaatcta
      721 tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt atttaaagca
      781 attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg aacaactagc
      841 agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat tggagttttg
      901 ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa tcgagtatct
      961 caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac aatttctcgc
     1021 acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag ttggtcctca
     1081 tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg gatcgcaagg
     1141 acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga ttaaagaaga
     1201 gacgggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg atgatagtcg
     1261 tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa caacaacgac
     1321 acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg ttcaacaagg
     1381 gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa
     1441 gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg taaacgacct
     1501 ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg ggaaattgtc
     1561 gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt gattattgaa
     1621 aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga tatccaggca
     1681 aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg aggaaaattc
     1741 ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc tgttgttaac
     1801 gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa
     1861 tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga tcgccatgga
     1921 acaacggttc actttacgcc agaccctgaa atttttacag agacaactga gtttgatttt
     1981 tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat gcgtatatcg
     2041 attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga cggcgggatt
     2101 aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga acctgtatat
     2161 ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac agacggctac
     2221 cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg aacacatgaa
     2281 tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg
     2341 atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt gacagctgtt
     2401 atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa attgggcaat
     2461 tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa attcttgctg
     2521 gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc cagagctcgt
     2581 ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga gattagtaat
     2641 ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga attgttcatc
     2701 gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc
     2761 atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga caagatcttg
     2821 gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg agaagatttt
     2881 gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga tgtcgacggc
     2941 gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc tattgttgaa
     3001 gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg gaagaatatt
     3061 acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt tgcttcactt
     3121 cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat ggatgatcat
     3181 caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt cagtgtggac
     3241 gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt agaaccccgt
     3301 cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta atcatgaagg
     3361 aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc taaccagcga
     3421 aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg cacttcctga
     3481 tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga acgaattagg
     3541 tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa
     3601 ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg cacagccatt
     3661 cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg acggtgacgg
     3721 tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg
     3781 tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag aaaaagaacc
     3841 agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt
     3901 aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg ctatcgattt
     3961 gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac ctggaccaga
     4021 ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg
     4081 taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa atggtaaaga
     4141 acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga tcgaacggat
     4201 ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg acgaatcttc
     4261 ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat ctgttatctt
     4321 gaataactta tataaattga catccttgca aaattcattt ggattcaata tgttggcgat
     4381 cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact atatcgaaca
     4441 tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc
     4501 acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca ttgcgattat
     4561 ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt tcgatttatc
     4621 cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg gtttggaacg
     4681 ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt taactgacat
     4741 tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag acttgaatca
     4801 acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa gtctggaaga
     4861 tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg gttacatcaa
     4921 acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg tccaaggaat
     4981 gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc atgatacatt
     5041 gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata
     5101 cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt cgagtgaaag
     5161 catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc tcttctttac
     5221 gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca tccgaagtaa
     5281 tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga
     5341 tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt tcaaagaaga
     5401 agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc tgcgtgagga
     5461 agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag tgatcacaga
     5521 aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa
     5581 agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat tgaccactgt
     5641 ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc gtttcaacgt
     5701 atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga agatggaagc
     5761 tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac aaacagttga
     5821 agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca agaatgaccg
     5881 attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct cttaaaatat
     5941 tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc agagcttgtg
     6001 agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc cgaaagtcca
     6061 cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt attcgtccaa
     6121 acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc ttgaaagata
     6181 acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga
     6241 acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca tcagcaaatg
     6301 ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt cacatgattg
     6361 ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg agaggagatg
     6421 actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc agatttgcgt
     6481 tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg taattttaca
     6541 aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg caaatcagca
     6601 gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg acgtatccaa
     6661 actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt agtagctgac
     6721 aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag cggcggattc
     6781 caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa tcaaacatct
     6841 caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt cggctcaagt
     6901 tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat aggagggaaa
     6961 tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact acattgctgc
     7021 taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat ttatttcaga
     7081 acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat
     7141 tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta gcgaagacta
     7201 ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc ctttttttct
     7261 gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt aaaatcaaat
     7321 tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc ccgaaacctc
     7381 tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta ccatttcgtt
     7441 ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat tggttgaaaa
     7501 tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg actgtagctg
     7561 aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct tatcagccat
     7621 caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat caaagtggat
     7681 taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag
     7741 tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt gttgtttatt
     7801 ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt cagccggtga
     7861 ttggtatttt atctattgat aactatgcag atgccattga taaattggat gacaaagaga
     7921 tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat gaataccatg
     7981 tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg atcgatctga
     8041 aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac
     8101 agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa
     8161 tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc gaccaagttg
     8221 tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa tctgctaaga
     8281 atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt cggatttttg
     8341 atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat gctttaggct
     8401 ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac atcgttatca
     8461 acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat aagcatcctg
     8521 aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa gaagaggacc
     8581 tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc ctatatgagc
     8641 aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac
     8701 ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt tctgagctga
     8761 tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca ttattgcttg
     8821 cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga acttttgacg
     8881 ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat ttattgagct
     8941 cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac tatgtgactt
     9001 ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt atagcagcaa
     9061 aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc attacaaagc
     9121 ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac gtccaaatca
     9181 tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa ctggaaaata
     9241 cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat atgaatcaaa
     9301 tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa aaggaaaagg
     9361 taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc taatcaaaaa
     9421 taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc
     9481 acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag aattcttgga
     9541 aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg
     9601 ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga
     9661 taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa acgtacctgt
     9721 taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag aataacaaat
     9781 cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa accgaaattt
     9841 atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc agattgattt
     9901 cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat aaaagagaac
     9961 tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc ttgtaaaatg
    10021 aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc gaatcccacc
    10081 gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg cagaaacgat
    10141 cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc atcaaattat
    10201 ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg ttactgtcaa
    10261 agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt atctctcaga
    10321 cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga
    10381 aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga aaggattcga
    10441 gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct tggaggtttc
    10501 ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact ccacgattga
    10561 aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa ctggctatca
    10621 aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc ttgctgcacg
    10681 tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag gaacaaaaac
    10741 agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg taaatcggat
    10801 gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc tttcggaaga
    10861 agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata tttttatcga
    10921 tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga
    10981 aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag gtaacggtaa
    11041 agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga
    11101 gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac agcgacaaga
    11161 caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag atgcggacat
    11221 cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg acgatgatgg
    11281 aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc gaagcggtgc
    11341 acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt cgctttcgcc
    11401 aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag ctctcgtaag
    11461 aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag atctccaaag
    11521 ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt tcaatcagct
    11581 gacccttggt attagctcag caaacgtcaa aatatgagga actattttga cgtttgtttc
    11641 tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg gtgttctcct
    11701 gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt ttttgaaatt
    11761 gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa
    11821 aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc tatttttgtt
    11881 tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc
    11941 caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga agcaattttc
    12001 gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt ttttaataac
    12061 cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa taaaaatcat
    12121 tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt aaataatata
    12181 gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg cgatgaaggg
    12241 aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg gtaccaaggt
    12301 ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact gcatttgatt
    12361 ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt tgtagtcaat
    12421 cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga aacaagtaat
    12481 ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt ggaccaatta
    12541 caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat cggacctgcc
    12601 tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga taaagaaatt
    12661 tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt aaaaatgttt
    12721 gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata cggacaacaa
    12781 atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga tgcaggcaaa
    12841 cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg aacttatcct
    12901 tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg tgtagggcct
    12961 tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt cggtgatgga
    13021 ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt aggacgagaa
    13081 tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt agttatgcgc
    13141 cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga tgtattaagt
    13201 ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact aatctaccac
    13261 tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga attgccaggc
    13321 tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa cgcacgtaac
    13381 tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc tgtcggacct
    13441 gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg ataaaaacga
    13501 cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata cgataataat
    13561 aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg tctcttgaac
    13621 actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct ttttccgaa
//
LOCUS       DAZKSR010000082        13606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000082 DAZKSR010000000
VERSION     DAZKSR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.082"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..716
                     /locus_tag="ACG8XG_002189"
     CDS             474..716
                     /locus_tag="ACG8XG_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFQ0774087.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            713..1183
                     /locus_tag="ACG8XG_002190"
     CDS             713..1183
                     /locus_tag="ACG8XG_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0774088.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1311..1391
                     /locus_tag="ACG8XG_002191"
                     /pseudo
     CDS             1311..1391
                     /locus_tag="ACG8XG_002191"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1398..2525
                     /locus_tag="ACG8XG_002192"
     CDS             1398..2525
                     /locus_tag="ACG8XG_002192"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0774089.1"
                     /translation="MSREYYNRIEKGKQPLNDELREIIEKQIERFNPQEPLFLLIDYF
                     RVRFPTTDALAIIRDVLQLKSNYMLYEDYGKYGYESKYVLGDINIMCSMQEHLGVLLE
                     LKGKGCRQMECYLLAQERSWYDFMLDCMTAGGVMKRLDLAINDKAGILDIPKLKEKYK
                     AGECMSYFRMQKDYSGTEKCGSDLPKNTGETLYLGSTSSELYMCAYQKNYEQYVKNGT
                     EIEDTEIKNRFEIRMKNERAYYAVVDLLTYRDAERTAFSIINHYVRFVDREDDKPKSQ
                     WITNDDWAWFVGENREPIRLTTKPEPYTLQKALHWLQRQVAPTIKMVQALDRENRTTI
                     LKDMIEQAELKDKHKHLLQLEKSTIEERIDTAVPQENDGIF"
     gene            3514..3723
                     /locus_tag="ACG8XG_002193"
     CDS             3514..3723
                     /locus_tag="ACG8XG_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HFQ0774090.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3835..5448
                     /locus_tag="ACG8XG_002194"
     CDS             3835..5448
                     /locus_tag="ACG8XG_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774091.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5483..6694
                     /locus_tag="ACG8XG_002195"
                     /pseudo
     CDS             5483..6694
                     /locus_tag="ACG8XG_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6717..7643
                     /gene="glsA"
                     /locus_tag="ACG8XG_002196"
     CDS             6717..7643
                     /gene="glsA"
                     /locus_tag="ACG8XG_002196"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0774092.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7851..9311
                     /locus_tag="ACG8XG_002197"
     CDS             7851..9311
                     /locus_tag="ACG8XG_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774093.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9532..11295
                     /locus_tag="ACG8XG_002198"
     CDS             9532..11295
                     /locus_tag="ACG8XG_002198"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0774094.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11379..11837
                     /locus_tag="ACG8XG_002199"
     CDS             11379..11837
                     /locus_tag="ACG8XG_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0774095.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11946..12629
                     /locus_tag="ACG8XG_002200"
     CDS             11946..12629
                     /locus_tag="ACG8XG_002200"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0774096.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12776..13390
                     /locus_tag="ACG8XG_002201"
     CDS             12776..13390
                     /locus_tag="ACG8XG_002201"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0774097.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13406..>13606
                     /locus_tag="ACG8XG_002202"
     CDS             13406..>13606
                     /locus_tag="ACG8XG_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774098.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFN"
BASE COUNT         4724 a         2160 c         2850 g         3872 t
ORIGIN      
        1 tttccgccat gcttgcaagg gggcggttcg cttcggcggt tcggcgagct tgcgagccgt
       61 tcaaagcccc cgttatctaa caaggggggc acaaaaaaca agaaacacta tacactaaca
      121 agcggaaaac gctgtattta taaggcaaaa ccccattttt acagtagtga tataaattgt
      181 acctatatca ctatgaaaat tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat
      241 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
      301 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      361 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      421 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      481 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      541 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      601 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      661 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      721 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      781 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      841 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      901 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      961 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
     1021 taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta catcgtatat
     1081 tcctataaaa gatattatcc tacactttga aaaatacgat tatcgttttc attggaatag
     1141 tgataattca gttattgaaa tttggatacc tattcaagat tagaagcaat tttatatcca
     1201 tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt tttaatgaca
     1261 ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga atgaatgata
     1321 aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca caaacaaggc
     1381 ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa ggaaaacagc
     1441 cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc aatccgcaag
     1501 aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg gacgcattgg
     1561 cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa gattatggaa
     1621 aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt tccatgcagg
     1681 agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg gaatgttatc
     1741 tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg gctggtggtg
     1801 tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat attccaaagt
     1861 taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag aaagattaca
     1921 gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc ttatatctcg
     1981 gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag cagtatgtca
     2041 agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata cgcatgaaga
     2101 acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct gaacgcaccg
     2161 ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac aagccaaaaa
     2221 gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga gagccgatac
     2281 gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg ctacaaagac
     2341 aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt acaacgatac
     2401 tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta ttacaattag
     2461 agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat gacggtattt
     2521 tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca ttccctatgt
     2581 gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc tacttgcgta
     2641 cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca ttaaatcggg
     2701 gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt tgaaaaaatg acggaaattc
     2761 gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat catcattttg tattcaaaca
     2821 ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata ctttgttctc aacttaacaa
     2881 tttaaggttt ccgttcccta tatggtgcaa agaaaatatt tcattctatt gttggcagta
     2941 acggtatggt ttgggtagtt agggtgtgaa aaggaaaata ttcttttttc atcatcaact
     3001 tagcaaaatg acattttcta tccctatatg gtgcatgaaa agaagttaaa atttttccat
     3061 ttaaacttag caaatggacg tttgctgtac agatagtagt gaaaaaggag aaaatcaacc
     3121 gttgattgcc aaaatgaacc gttccatttg tactaataat ggaaaaagtt ttggattttg
     3181 gttacgtttc tcctcttttc caacgcgata tataggaaag acgatttttt tctttcaatc
     3241 gttctttgac aactgaataa agctattcaa tacgtttgac agacagctag ccgttgaaac
     3301 aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa cctatttgtg ttcttgtaaa
     3361 agatttgtcc tcttttatcg ccatgacctg tactgtcaga taatgatact cccgtacagc
     3421 cacagcttga gcattaagag cgtcgcacgc aatgggtacg gctacataag aactatgcag
     3481 aggtggaact cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga
     3541 aagggggtat gtgttattga taatgctgta aaaacaattc aaaagaaaaa tggcaagtgt
     3601 ggcattggca acagaggaaa gacaaagatt gtagtaaaag agcatttttc cgaaaagggc
     3661 aaaacaatgg aagaacttct aactgatgtc atgctggaaa aagcaaagca gacaatcgca
     3721 taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt gttatacttt
     3781 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
     3841 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacagggc
     3901 gaaagttcca gcattaccac acagagaagt atgttgcgtt tatatgcaaa agaacatcat
     3961 ttgaatgtca ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgacagaccg
     4021 agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
     4081 gacctttccc gtcttggcag aaattatatt atgacaggac aatatacaga attgtatttc
     4141 cctagtcata atgtccgcta catagcgatt gatgacggtg tagacagcga aaaaggcgaa
     4201 agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga tacaagccgt
     4261 aaagtcaaat cagcatttaa gacaaagttt gcggaggggg ctcattacgg tgcttatgct
     4321 ccgttaggat ataagaaaca ccctgacatc aaaggaaaac tgttggttga tgatgaaaca
     4381 aaatggatta ttgaaaaaat cttttcccta gcctatcaag gttacggaag tgcaaaaatc
     4441 acaaagcagt taagagcaga aaaagttccg acagcgtcat ggctaaattt tacaagatac
     4501 ggtacttttg cacatatctt tgagggaaaa cccgaaagta agcgttatga gtggacgatt
     4561 gctcatgtca aggcgatatt gaaaagtgag gtttatatcg gaaacagtgt tcacaatatg
     4621 cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta agcccgaaag tgaatggttt
     4681 cgagtagaaa acactcacga gccgattatt gacaaggaag tgttctatcg tgtgcaggag
     4741 cagataaaat caagacgcag acagacaaag gaaaaggcaa cgccgatttt tgcagggctt
     4801 gtcaagtgtg cggattgtgg ttggtctatg agatttggaa cgaataagac aaataaaacg
     4861 ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtaa ttgttctatg
     4921 cactacattc gttatgatgt gctgtatcaa gccgtattgg aacgcttgca gtattgggct
     4981 aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc tggcaatgca
     5041 gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga gaacagacaa
     5101 aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
     5161 gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga actggaacag
     5221 cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat aggtgctgaa
     5281 aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc accattatta
     5341 aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga taacgaaaga
     5401 atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc aacaagagta
     5461 atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     5521 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     5581 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     5641 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     5701 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     5761 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     5821 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     5881 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     5941 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     6001 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     6061 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     6121 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     6181 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     6241 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     6301 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     6361 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     6421 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     6481 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     6541 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     6601 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     6661 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     6721 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     6781 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     6841 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     6901 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     6961 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     7021 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     7081 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     7141 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     7201 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     7261 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     7321 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     7381 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     7441 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     7501 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     7561 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     7621 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     7681 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     7741 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     7801 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     7861 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     7921 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     7981 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     8041 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     8101 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     8161 gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca atcaaaaaaa
     8221 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     8281 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     8341 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     8401 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga gaaatagaga
     8461 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     8521 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     8581 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     8641 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     8701 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     8761 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     8821 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     8881 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     8941 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     9001 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     9061 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     9121 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     9181 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     9241 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     9301 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     9361 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     9421 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     9481 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     9541 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     9601 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     9661 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     9721 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     9781 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     9841 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     9901 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     9961 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
    10021 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
    10081 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
    10141 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
    10201 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
    10261 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
    10321 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
    10381 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
    10441 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
    10501 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
    10561 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
    10621 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
    10681 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
    10741 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
    10801 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
    10861 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
    10921 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
    10981 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
    11041 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
    11101 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
    11161 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
    11221 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
    11281 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
    11341 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
    11401 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
    11461 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
    11521 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
    11581 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
    11641 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
    11701 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
    11761 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
    11821 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
    11881 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
    11941 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
    12001 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
    12061 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
    12121 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
    12181 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
    12241 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
    12301 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
    12361 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
    12421 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
    12481 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
    12541 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
    12601 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
    12661 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
    12721 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
    12781 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
    12841 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
    12901 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
    12961 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
    13021 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
    13081 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
    13141 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
    13201 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
    13261 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
    13321 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
    13381 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
    13441 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
    13501 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
    13561 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaac
//
LOCUS       DAZKSR010000083        13449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000083 DAZKSR010000000
VERSION     DAZKSR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.083"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..533)
                     /locus_tag="ACG8XG_002203"
     CDS             complement(207..533)
                     /locus_tag="ACG8XG_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774099.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(545..745)
                     /locus_tag="ACG8XG_002204"
     CDS             complement(545..745)
                     /locus_tag="ACG8XG_002204"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774100.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            868..1470
                     /locus_tag="ACG8XG_002205"
     CDS             868..1470
                     /locus_tag="ACG8XG_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0774101.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1525..2691
                     /locus_tag="ACG8XG_002206"
     CDS             1525..2691
                     /locus_tag="ACG8XG_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774102.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            3108..6335
                     /gene="fss3"
                     /locus_tag="ACG8XG_002207"
     CDS             3108..6335
                     /gene="fss3"
                     /locus_tag="ACG8XG_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0774103.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6447..6761
                     /locus_tag="ACG8XG_002208"
     CDS             6447..6761
                     /locus_tag="ACG8XG_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0774104.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            6774..7148
                     /locus_tag="ACG8XG_002209"
     CDS             6774..7148
                     /locus_tag="ACG8XG_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0774105.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            7158..7502
                     /locus_tag="ACG8XG_002210"
     CDS             7158..7502
                     /locus_tag="ACG8XG_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0774106.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            7571..8587
                     /locus_tag="ACG8XG_002211"
     CDS             7571..8587
                     /locus_tag="ACG8XG_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0774107.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            9181..11055
                     /gene="ltrA"
                     /locus_tag="ACG8XG_002212"
     CDS             9181..11055
                     /gene="ltrA"
                     /locus_tag="ACG8XG_002212"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0774108.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            11157..11537
                     /locus_tag="ACG8XG_002213"
                     /pseudo
     CDS             11157..11537
                     /locus_tag="ACG8XG_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            11582..12724
                     /gene="dcm"
                     /locus_tag="ACG8XG_002214"
     CDS             11582..12724
                     /gene="dcm"
                     /locus_tag="ACG8XG_002214"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFQ0774109.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            12714..13003
                     /locus_tag="ACG8XG_002215"
                     /pseudo
     CDS             12714..13003
                     /locus_tag="ACG8XG_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13259..>13449
                     /locus_tag="ACG8XG_002216"
     CDS             13259..>13449
                     /locus_tag="ACG8XG_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774110.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNP"
BASE COUNT         4574 a         2309 c         2740 g         3826 t
ORIGIN      
        1 agaattagaa tagccttgct tttcaactaa gctttcaact tctttttcat tcattgtcac
       61 acctgcttct gattttattt gctataatag attcaaataa agttttctca gaactttatt
      121 attgagtgtt cccccgatct gcaaactgac gggaacactc tttttaattt tttctacttc
      181 atcaaattta aaaaacgagg tgttttttaa atttgatgac tcaatatcca ttttttgcat
      241 tcttcttttg aaaacaactc cattttttct aataaaataa ctggcaatcc cattctttta
      301 aatttcaaaa aagttgtata tgatacattc aaatatttag ccatctcttt tccttttaga
      361 aatggtcttt ctaatccggt atcaattctt gcttgatcaa tagcctcttt tgtcatttca
      421 aaaacataat ttttcaattg attcgtttgt tgtttatcta acattactgt aattcctgca
      481 tccattattt ttctcctctc attttctatt tcttcaatca gccaactcgc caagttgtat
      541 ttatttagat tttcaaatca ttaagtaacc aatttaccaa cttttgataa gtagatgctc
      601 taatactttc tttgctccca ttttcgattt tgacatatgt tttccgattt atacctatca
      661 attttgctgc atcgttttta gtaagtataa gatcggctct atgtcttctt aagcttctaa
      721 tgaattgatc attaagagtt atcattcagc gcctcctttc aaattattca ctttgtggtt
      781 tttatcttaa taagcacatt ttaaaccaca aaacgagtaa tatcaattaa aatattactc
      841 aatttgtggt tttcatgcta taatagtttg gaatttacta tagggggcgt tactttgaat
      901 agaataaaat tttatattga ttccagtaaa aagaccatta aacaaattag tacagaaact
      961 ggtattaact atacaactct tacgaactat taccgtggtg agcgaaatcc tagaaatact
     1021 gaaacatgga atatccttgc tgattatttc catgttcctg taagttattt gatgggatta
     1081 gacgaaacaa attccataga acaacctaat cttgatagaa taaaaactgc tcgtttagaa
     1141 aaaaaggaca aaatagtcaa gaatattgcc agtcttgact cagattattt tactgaatta
     1201 tctaatctat acttttccat gacttcttta cttgaagtca aacttcaaga agatgactcc
     1261 gactttcttt ttttaaaaag tattctagag gactcctttg aatacacgat tctatttgcc
     1321 aaacaaaaga agtttattaa taaagcttta aacttaaata tcgatgaaag aaaagagtta
     1381 gaatctgagt tatttggcaa cttaacccaa gtacaggtta acaatctaaa aagagagata
     1441 acagctcgat taatatcaaa acttcaataa aactaattat tcaatcagcc aactcgccaa
     1501 gttatcagat tggagagatt agttatgtca atacaaaagt acacaacaaa aagcggtgca
     1561 cgatatagtg tccgcttgta tgttcgtaaa gatgaaaatg gaaagcaaga atactatgta
     1621 aaaaaagggt ttaaaactga aaaagaagcc aagttacacg aagcaagaaa aaaggtcgag
     1681 attgaagata ccggctatca taagccttct aatgacttgt ttataacagt gtatgaagaa
     1741 tggttgcctt cttataggaa tcgtgtacaa gaaacgacat atcaacgtac aaaagacctt
     1801 ttccgcttgc atatactacc aaaattcggg aataagtcca tttctaagat tactcctgtt
     1861 cactgtcaga atgcagtcaa tgaatgggct ttaaaattca agaatatcaa acagcttaag
     1921 tcttacagct ctcaaatatt tgagtatgcc atttttgcag agctattaca ccgaaatcct
     1981 atggcaaata caaatctacc aaagcgggaa aagaaagaaa atgaaaatta cttttcatta
     2041 gaagaactaa aagctttttg ggatattctc aataaagaag agcctttaaa acatatttta
     2101 atcttccagc ttttgattac tactggtata cgtaaaggag agctagctgc tcttcattgg
     2161 tctgacattt attttgaaga acagctaatg tatgttagaa agagctatgc aactattcga
     2221 aatgaagaga agaatgcaag aagaaaaact gtcaggattc aaaaaaatac aaaaaatata
     2281 tcttccgaac gaatattacc aattgatgca caaactattg aaatgttaaa aatctggaaa
     2341 aaagaacaag ctcgagagct tttgcagttt ggcataaata caaatacaaa aaatcaactt
     2401 atctttacct atgtaaatgc agacggtgac attaatcaac ctttgcatgc ggactattca
     2461 aataatatat tgaaacgttt agagaagaaa tataaattta agcacgttac tattcatgga
     2521 ttccggcaca ctcacgctac tcttctacta gaaggtggag caagtatcaa agaaacacaa
     2581 gatcgattgg gtcataaaaa tgctgaaact acccttaaca cttattctca tgttactgaa
     2641 aaagctcaac gaaatgcgtt agaaaaattc attgcttaca ctggtttata atattgtcat
     2701 tacctttctc attaccagaa cgaaaaaaag acaccttctc gcaatgagaa agtgtctgaa
     2761 atcgttggta taacaacgat taacgttttg aaaactgtga agctttacgt gctttcttaa
     2821 gacctggttt tttcttcgta tcattgaata agtttgaaaa gccttgattt ataaaggttt
     2881 ttccgtaaat atattttgtt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg
     2941 ttctaattta aaggcagtat atttcgtatt ttatattaaa tttttaggct cattcattct
     3001 caaatccatt ctatttagtc aataaatatt ttaaaaagac tttataaaca aagatgttca
     3061 agcgagcatc ttattttttt gtgcctaatt tgaaaggaga acatttcatg tataaaaaac
     3121 aaaaaaagaa attatgtgta ttaactattt tgagtttggc tgttcaatca attgtaggag
     3181 caattgctcc tacgattgct tttgctgatg aaattaccca tccacaaacg gtaaccgtag
     3241 aactagattt agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt
     3301 cagaagtcac tgttccgcat tatgcagtgt acaacggcgt taaacaagat attttctgtg
     3361 ttgaaccagg agtcccgatt tacaatgagt tcactcctgg atatgagaag aacccgttac
     3421 cagatatgtc cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg
     3481 cagatactca tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc
     3541 actcaataaa acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaatca
     3601 atcaagccat tgccgattat cagaagaaac caagcttcca taatagtacg gctaaaacag
     3661 tattagggca atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg
     3721 aggttgtgga aaataccgca aacattgatt atcgtgtgaa tggcaatcag ttgataatca
     3781 gcccaaatgc caattcaaaa gaaagtggtg tgttgacgct taagaaatct gctggtaccg
     3841 gaacacctgt cgcctataaa aaggctggcc aacagacttt aatggccggg gcgatcgata
     3901 agccgaacac ttatacggtc aaaattgatg tggaaatgga gggttctttg aagattaaaa
     3961 aagttgataa agaatcgggt gatattatac caggaacggt tttccattta gactttggaa
     4021 aaactttacc cgcaaaagac gtgaccaccg ataaagaagg cattgcgacc ttagatgaga
     4081 ttcctcatgg tacaaaggta accattactg aaaaatcggt gccggcgcct tatacgattg
     4141 ataccacacc catgactgcc acaattaaag caggtgaaac catttcagtg acttcaaaaa
     4201 atacgcgaga aaaaggtcaa attattctgg ataaaaccgg aattgaaaca gggagtgatc
     4261 tttggaacga taattactct ttagcgggca atacgtttgc cattcgcaag gatagcccaa
     4321 ctggtgaaat cgtccaagaa atgaccacgg atgaaaaagg tcatgcagaa acaccaaaag
     4381 agattgccaa tgcgttggaa ttgggaacct actatgtgac tgaaaccaag gctagtcatg
     4441 gctttgtgaa tactttcaaa ccagtaaaag tcgaattgaa ttatgtcaat caaacagtgg
     4501 ctcttgttac cagtaacgta aaagggcaaa accaagaaat tactggtgaa accactttga
     4561 caaaagaaga caaagatacc ggtcatgaaa ctcaagggaa agctgagttt aaaagagctg
     4621 aatacacctt gtttactgtt aaggatggaa aagcggtcaa atggaccgag aactttaaac
     4681 cagaattgtt gaagggaacg aaagcttctg atgaaaccgt aactttgact ttagatgaaa
     4741 agaatcaagt tgccgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag
     4801 cgcctgaagg atataccttg gatgaaacaa agtatcctgt atccatcaaa aaggtggatg
     4861 atagcgaaaa aaatgctgtg attactcgag atgttgcggc aaaagaacaa gtgattcgct
     4921 ttggctttga tttctttaaa tttgctggat caactactgg cactgccgaa actggcttca
     4981 atgatctaac ctttaaagtg tcgccattgg aaggaaccaa tgaaatcacc ggtgccgaag
     5041 atgaagcgac cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa
     5101 atcttcctta tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga
     5161 ttacgccatt agaaatccgt tctactttta aagaaaataa agaggattat accaagagtg
     5221 attatatctt tactattacc gaaaaagggc aaaagcaacc gattaaaacg gtaacggtcc
     5281 cttatgggaa tctaaccaat gagaatttct cagtcagttt gaaccgtttg atgctttatg
     5341 atttgcctga ggaagaagat agcttaactt ctcttgcgac ttggaaagaa ggagataaag
     5401 aattgactac tcttgattct accgagctag tcgataaatt gagctataat ctacatgaaa
     5461 tcaaagaaga ctggtatatc gtagctcaag ccatcgatgt agatgctaca aaagctgccc
     5521 aagaaaaaga taaaaaagcc aaaccggtgg cgattgcgga aacaaccgca actttagcga
     5581 ataaagagaa aacaggaact tgggagattc tgcataaatt aactgtcgaa caagttttag
     5641 acaagaccat tgtcttattc aactatgtgt atgaaagcaa agaagcctat gaagctggtg
     5701 atgagccggt agcgaaggat gctagcttga acaatcaagc gcaaactgtt aattgtatgg
     5761 tggaacgtca tgtttccatc caaaccaaag cgcacttaga agaaggttct cagaccttta
     5821 ctcatggtga cgtggtagat atgtttgatg atgtatccat tacccatgat gtactagatg
     5881 gctcaaaaga agcttttgaa acaattctgt acgctttatt tccagatggt acaagtaaag
     5941 aaatttggaa atctggcaag attgattacg aagtgaatga caaagaattt accaaaaccg
     6001 tacttgcgga aaaagtggat acaagtaaat atccagaagg tacaagtttt accttcaaag
     6061 aaattaacta tgacaaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa
     6121 aatctcaaac tttaacgccc aaaaaagttc cgacaacacc aagtacacca gaacaatctg
     6181 aaatgccaac tgttccaagt ggctctcaag aatctagtcc tacagtgaag gcattccctc
     6241 aaaccgggga gaaaacttcc aatgtgctac tgttcattgg ctttagcttg atctttgcga
     6301 cggctggcta ttatttttgg aatcgtcgga actaaggtga taagatgaaa catgtaaata
     6361 gtagaaaaca atacgcgcga ccgcctccta aaaaagtggt cgcgctattt ttgtattcaa
     6421 aactaacaat gaaaagagga aaaaatatgg aattgaaatt tgttgtacca gatatggtcg
     6481 agacgttcgg aaaactaaca tacggtggtg aaggtgaagt tctatcggaa gggtatggtc
     6541 gaaatactac aattgttggt cgaagttacc atctgttttc aaataaacaa cgggccgatg
     6601 atattgaagt ggtggtagct gcggaagccg gcgaaaagga ttttgaccaa gatcaaccac
     6661 taaaagctgt aaatccccac ttaattgcca aaggctatga aattgagaat cgtgggttta
     6721 ccgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat
     6781 taacagaagg tattgtcgta gattcaggat taacctttgg caagttgcgt ttttccgcat
     6841 tacgtcgaga agtacgcaag caaaatgagg acggaacggt tagtaacgag gtaaaagaac
     6901 ggacctataa tttgaaatcc tccgcgcaag gtcgaatgat ccaagtcagt attccagcaa
     6961 atgtaccctt acgcgagttt gcttatgatg cagaggtaga gctggtcaat ccaatcgtgg
     7021 atacggtcgc caattatgtt tttcgggaag gaaccaccgt taattggttt atcaaggcag
     7081 atgatttagt cttgaaacga caactgaatc aaggaaaacc tacgaaccaa aatgaaggaa
     7141 agaaataggt gaagaatatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat
     7201 ttggcaaagg tgagctaacg gaggaacaac tcactaacgc attaacctat gatgaaaaaa
     7261 tgtttctgat tatgcatcaa gggttactgg aagtaggtaa cgatgccaaa gaagactttg
     7321 atgtactgga ttggttaggg gaagaagagt ctttctttat ggttgtcgag gtaaatgaaa
     7381 gtttatgtcg gcaggcagaa tctgttttgg aagaaatcga tgtggagatg ccggatgcca
     7441 tcgaaagatt tttaaaacaa ttagtggaaa cgaaacaact tcccgtagta gtaaatgact
     7501 agcataactt tttgaaattg cccaatgaaa tcctattggg ctttttcgac tggaggtgga
     7561 aaccatcaaa atgtacaaag gacatcgcat acgcgcagga gatcaacact tgatttatca
     7621 ctttgttctt ggctggctaa tctttttgtt cattggttgg atgagcgtct tttatttaca
     7681 agaacttaga caatttgata tttctaaggt atcattttcc actagtgaaa ttggctggtc
     7741 tatgaaggat gtaatctgct tgctaggaag tctcgctctt tcaggaagca tgatgttact
     7801 ttatatacac ttttttcagg atcattggcg aagtctttgg catcgacaaa agttagctcg
     7861 gatgattcta gaaaaccatt ggtatgaagt aaagcagact caaggtgaag gttttttcaa
     7921 agatttgaat agtagttgga ccaaagagag tatcagttac ttccccaaaa tttattatcg
     7981 aatgaaagat ggcttacttt ctattcgcgt tcaaatttca ctgggaaaat atcaggatca
     8041 gctattgaag ttggaaaata agttagaaag cggactgtat tgtgagttag tagaaaaaga
     8101 actcaaagac tcctatgtgg aatacacctt gttatacgat atgattgcca atcgaattgg
     8161 gatagaagaa atagtggcag aaaatggggc gttgcgattg atgaaaaatc aagtatgggc
     8221 gtatgattcc ttgccgcata tgttaattgc tggcggcaca ggtggtggga agacctattt
     8281 cttactcacc attattcaag cactattgaa gtcagatgca gaattgttta tcctcgatcc
     8341 caaaaatgcg gacctcgccg atttaggtac gataatgcct cacgtatact cccaaaaaga
     8401 agaaatttct gaatgtgtgg aagattttta cgaacgcatg atagctcgta gtaggtcaat
     8461 gaaagaaatg cccaactaca aaacaggaga gaattatgcg tatctcggac ttccgcccaa
     8521 ctttttaatc tttgatgaat acgtggctta tatgggtgcg aatcgttttc ctacaagcat
     8581 cgaatagatg tgaaatggaa gcctattgag attatcaata gtgacaacta atgttttgag
     8641 agatggaatg aaagccttca agttgagctg aaacatcccc gctaatactc ctacatgctc
     8701 taatcaatga agtagattgg ttttggtatg aagctaggtg aagtcgtctg aaatctgcct
     8761 aagtgcttga gcataaggct gactataggg cgggcggctg aaaaagataa ctatggtaag
     8821 aatgtctgaa aatgactgac gaatctgcga atgtacgggt ctaaaaggct gatcttattg
     8881 aaagatagga acatacggac gtatggtgag ataccgacaa gtaaggttcc atgatatgaa
     8941 aatcgggata gctaacgaag aagctataat ttcacaggct catagtagat acctaaggtt
     9001 atacatgata gctaaaaaca ttggaacgat gtaagcagaa aactatcaat taaatgacta
     9061 cttctagata ggtgcatata agtaataacg aaattgcttt gtttctgtga aagtaagggc
     9121 acagtaccga tgaagcgtct aacaaatgtg gagggatagc cctaagtcta tttaataaca
     9181 atgaatgttg aaacagatga agttcaagga tctggtaaga acgtgggaaa atccgttatg
     9241 gaggtgccac gcttgaacac aaaaatacgt tactgggaat attatgatct acaagaaaca
     9301 ttcgatcagc tatttactca aagtagaaac gggaaaaagt tttatcaatt atatgagtta
     9361 atcatttctg aaaataatat tcttttagca taccgtacta tcaaggcaaa caaggggtct
     9421 tcgacaccag gaacggattc cttcacaatt gataactata aggaaatgaa ccaagcagag
     9481 tttatccatc ttattctcag tcatttagaa aattacaaac caaaatccat aaagcgagta
     9541 atgataccta aacccaatgg tgagaaacgc ccattaggaa ttccatgcat gattgatcga
     9601 atgatccaac agatgttcaa gcagatactg gagccaatat gtgaagctaa gttttatgaa
     9661 catagctacg gtttcagacc attgagaagt gcaaagcatg ctctaggtcg cattatgtat
     9721 ctcataaaca tcagtaaaat gcactatgca gtagacattg atatcaaagg tttctttgat
     9781 aatgtaaacc accgtttact gataaaacaa ttatggaata taggaatttg tgacaaacaa
     9841 gtcttagcta ttctttcaaa atctctgaaa tcacccattc aaggagaagg tatcccgagc
     9901 aaaggaacga tccaaggagg tatcatttct ccactattat ctaatgttgt tctaaacgac
     9961 ttagaccatt gggtctcaaa gcagtggcat acctttgaaa caaagtatcc ctatactaaa
    10021 ggttacaata aatttcgtgc tctaagagat acaaatctaa agcaaggcta tattgtcagg
    10081 tacgcagatg actttaaaat catgactaat gattatccga cagcattaaa atggttccac
    10141 gctgtcaagc tttacctaaa agacagactt aaattagata tttccaatga gaagtctaaa
    10201 atcgtcaatt tgcgtaaatg taagtcggaa tttttaggat tcgctatttg cgtgaaacaa
    10261 aaagggaaaa aatgggtttg taattcacgt atttctaaca aaaaaaagga tcagattaaa
    10321 gaagagatta aacaaagaat caaagatatt caaaagagtc ctacagcaca aaatgctctc
    10381 ctgtttaatg ctctcgtgct cggtgttcat aattatttca ggtatgccac tcatattaat
    10441 ttagatttga ataggatgaa ctacgttctt tcattcacta cttacaaccg attatcaaat
    10501 tgtagtacaa gatgttaccc caacaaagct tccccaactt acaaaaagtt ttactcaaca
    10561 acgatgaaaa cgtataaagt agctggagtc tatttgtacc ctctttgcga cgtatctaca
    10621 aaaacaattt acggatttaa tacggaggat acaccgttta ctccgtttgg tcgtcaaaga
    10681 ttggagcaca aaagcctaca gtctcttgtg ttccaagaat taagaaaatt gatggaatcg
    10741 aaaataccga atagaacggt tgagtattta gatagtagaa tatctcgtta ctcaatgaaa
    10801 tcaggcaagt gtgagattac aaaacaattt ttgccagcaa aagccgttca ctgtcatcac
    10861 tatttgccaa aaagtcttgg tggagacgac aaattcgata acttgagaat tattcataag
    10921 gatatccatc ttctgatcca tacaacaaat aaaatgataa tcgatcacta cgtgcatgaa
    10981 ctgaaactgg tgccagagca aatagcaaaa atcaaccttt atcgaaagat gtgtaatctg
    11041 caaaacattc aatagaaaat aaatagatgg aacgccgtat gaggtgaaag tctcacgtac
    11101 ggtgtggagc gggggaaaag atggagatta tatcaaattc ttacctatcg ctatagatgt
    11161 taacgacaaa agaaagtgcg gtgattttga ataagcttaa acaaatcgtg atgttaggcc
    11221 gtcaatctgg tttctttctg attctagctt gtcaaaggcc ggatgcgaaa tatctaggag
    11281 atgggattcg cgatcagttt aacttccgtg tggctttggg tcgtatgagt gagcttgggt
    11341 attccatgat gtttggagaa gtcgataaaa atttttttat gaaacgcatc aaaggacgag
    11401 gttatgtaga tacagggggc agtgtgatta gtgagtttta tacgccactt gtaccaaaag
    11461 ggtatgattt cttaagggaa attagcaaca ttgttggatt aaccgttcat acggaaagag
    11521 aaaataatag tgtgtagcct aggaatatca ataatcctgg gcttttttat tgaggtgatt
    11581 tatgacattt ttagatttat tcgccgggat tggtggcttt cgcttaggta tggaacaagc
    11641 cggtcaccag tgtatcggtt tttgtgaaat tgatgaattt gctcggagga gttataaagc
    11701 aatccatgat acaaggaagg aggtggaaat gcatgacatc acaagtgtat cagacgaatt
    11761 tgttcgatcc ctcggaccag tggacatcct ttgcggcggc tttccgtgcc aagctttttc
    11821 aattgcggga aagcggcaag gattttccga tactcgaggt actttattct ttgaaatcgc
    11881 tcggttcgcc actcttctcc aacctaagtt tctattcctt gagaacgtcc ggggattgct
    11941 caatcacgaa ggaggggcta cgttcgagac gatcctccga acgttggatg gattggggta
    12001 tgatgtggaa tggcaagtgc ttaactcaaa ggcctacgtt ccccaaagcc gtgagcggat
    12061 cttccttatc ggacattctc gagatgcatg taccgaacaa gtatttccta tcattggatc
    12121 gtcttcaaca tctgatcaaa acatcagaaa cttggtaaat atcaatcctt ccaatcgagg
    12181 aatgggtggc caagtctatg gttcagatag cgtagcacca accttgaccg gtgatgaagg
    12241 aattaaaatt gccttaccgg taaaggatgg gatttctgta gcgggtatgt tgtcgggaaa
    12301 ttttgaacaa ggcaatagga tttatgaggt aaccggaaca gcccccactt tgtcaacgaa
    12361 acaaggggga acgaagatta tgattcggaa aaatgaaaca gaatatcaag aggtgaagcc
    12421 aggggacagt gtgaacctgg cttttcctaa ttccacaagt cgccgaggtc gtctgggaaa
    12481 acaaagtgtg cataccttac taacaggaga ccaacaagcg gtagtcacgg atcagtacca
    12541 gattcgaaag ttaacaccta gggagtgctg gcggcttcag ggctttccag attgggcttt
    12601 tgatcgagct tctcaagtca attcagatag tcagttgtat aaacaggccg gaaactcagt
    12661 aacagttccg gtcatttttg atattgctag aagattaaag ggggtaaaag catatgattt
    12721 atgacgagtt gattggagaa atctactgga tgattggcaa gattcaggca gatccgaaat
    12781 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccagg
    12841 tgattcgtat gtagtggatg aggaaacgga tgctcgaatg gagctcaatc aagtcatcgg
    12901 tgaattggaa cgaattgcgg accttgccaa agaacctgat atccgccaat acttgtttga
    12961 gataaaagca gagttggaaa ttgagggtat caccgcggag taatagggaa ataacccact
    13021 gataggcact gaccgcgggg gatttgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt
    13081 catggacggc ggcagccggc catgatgtct caccccccgt atctaacagg ggggtacaaa
    13141 aacacaaaaa acaagcaagt aaccataaaa aatgcttgtt gtatcaagga tattaagtaa
    13201 tttgtatagg tgttaactca tttggaaaag ttaacacctt gaatttggag gtgaaaactt
    13261 ggcgcaacga aatttagatt atcggttatt aaaagaacga cgcaacgaat atggcgtttc
    13321 acaaaataaa ttagctactg cttgcggact tagccgccca tacctaaatc agatagaaaa
    13381 cggtggcgta atcgcctcta caaagaccat gagaaaaatt tttgatcaac tggaaagttt
    13441 caatcccga
//
LOCUS       DAZKSR010000084        13423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000084 DAZKSR010000000
VERSION     DAZKSR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.084"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..624)
                     /locus_tag="ACG8XG_002217"
     CDS             complement(<1..624)
                     /locus_tag="ACG8XG_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774111.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELI"
     gene            complement(635..1303)
                     /locus_tag="ACG8XG_002218"
     CDS             complement(635..1303)
                     /locus_tag="ACG8XG_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFQ0774112.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1434..2018)
                     /locus_tag="ACG8XG_002219"
     CDS             complement(1434..2018)
                     /locus_tag="ACG8XG_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0774113.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2353..2583)
                     /locus_tag="ACG8XG_002220"
     CDS             complement(2353..2583)
                     /locus_tag="ACG8XG_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0774114.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2598..3548)
                     /locus_tag="ACG8XG_002221"
     CDS             complement(2598..3548)
                     /locus_tag="ACG8XG_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0774115.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3545..4387)
                     /locus_tag="ACG8XG_002222"
     CDS             complement(3545..4387)
                     /locus_tag="ACG8XG_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0774116.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4520..5245)
                     /locus_tag="ACG8XG_002223"
     CDS             complement(4520..5245)
                     /locus_tag="ACG8XG_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774117.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5706..6020)
                     /locus_tag="ACG8XG_002224"
     CDS             complement(5706..6020)
                     /locus_tag="ACG8XG_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0774118.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(6036..7631)
                     /locus_tag="ACG8XG_002225"
     CDS             complement(6036..7631)
                     /locus_tag="ACG8XG_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFQ0774119.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7654..8544)
                     /gene="iolE"
                     /locus_tag="ACG8XG_002226"
     CDS             complement(7654..8544)
                     /gene="iolE"
                     /locus_tag="ACG8XG_002226"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFQ0774120.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8561..9586)
                     /gene="iolG"
                     /locus_tag="ACG8XG_002227"
     CDS             complement(8561..9586)
                     /gene="iolG"
                     /locus_tag="ACG8XG_002227"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFQ0774121.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9610..10617)
                     /locus_tag="ACG8XG_002228"
     CDS             complement(9610..10617)
                     /locus_tag="ACG8XG_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0774122.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10730..11890)
                     /locus_tag="ACG8XG_002229"
     CDS             complement(10730..11890)
                     /locus_tag="ACG8XG_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFQ0774123.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11966..13420)
                     /locus_tag="ACG8XG_002230"
     CDS             complement(11966..13420)
                     /locus_tag="ACG8XG_002230"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFQ0774124.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         4022 a         2898 c         2198 g         4305 t
ORIGIN      
        1 aattaattct ttcaaatagt aaaaacgatg ttcagctata acaatagtaa atcctttatt
       61 ttttaaatca caaagtaagt cagcaagttt cttagtcgct ttgaagtcca aatttgaaga
      121 cggttcatcc aatataatta atttagagtt tagcgataaa ctacatccaa tagaaatttt
      181 ctgccgttca ccacttgata gcgaaaaaat gtttttatct aatatatttg aaagatctaa
      241 taattcagct aattcattga ttcttttatc catatgctct tttgtaaatc caaagttttc
      301 catcgaaaat actaactcac tcgttgtatt tgttgtaaag aattgtcctc taggatcttg
      361 acttacgtag ccaatatttt ttgatatact ttcttgtgtt aaggattgta gcgagttatt
      421 ttcaaataaa atttccccaa ccaattttcc gtcataaagt cttggaataa gtccattcat
      481 taactttaat agtgtggatt tcccactccc agaagaacca gtaatcaatg taagactacc
      541 tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa
      601 agaaacattg ttaaattcaa acaattcttt cacctcaaat caaaatactt aagaaaaaaa
      661 tagtaacgga aaacaggatg atatagtcta ataagctcat gtttgtttta tagtaagaac
      721 ttcttttcac aggagattct atcccctttg ttattgcatt agcggtcatt tcttcagcaa
      781 tcatagttgc acgtataaga acgggaacta acagaaattc caaatattgc agaggctgaa
      841 aaaaagacaa gcgaatgttt ctcattttta aagattcttt aatcattctt atttcagaac
      901 gataagtcgg aaagaatctg atcactactg taatacttaa cattattttt ttcggtaaat
      961 gtattttttc taaagaggat aataattcat tgctagtata gacactaacc aacaaccatg
     1021 agatcattac gaccggcaac attcgaaata caatatataa ggatgtccca aaaaatatag
     1081 taaaaaactc tatcttgata gcatcaataa ttttcattac taaaaacaga actgaataaa
     1141 tcaagatgaa ataaatacat gtctttattt tacgaaacat caatactaaa actgtagcaa
     1201 taatgaaaca ccccacttga aatatatttc cctttcctaa aaaaataatg accggaaaaa
     1261 aaatatttaa agtaatcaag gttaacggat tgatttttga cactctttca cctccaaaaa
     1321 ataagatcgc actgttatct taagctgata aaaatgcacc gttattcaga taacagtgcg
     1381 atcttatcaa aaagcataac tgagaacaat tctcaatgca agcttttttt taattaactc
     1441 agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc
     1501 ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg
     1561 tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt
     1621 aacttatcta gacaacagac tgtctttttt gtcacaaatg atgtaatttt ttcaacaata
     1681 tcttgatatt tggttccagc tgattggaat aaaaccaaat cgaatatacg tttattctca
     1741 aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact tttctctata
     1801 tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc
     1861 gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta
     1921 taaaaagcac ccgtagttac gcctgcggtc aagcacagtt gccttatctt agtttgctga
     1981 aatcctactt tttcaaaatg gcttagtcca ctttctatta atttttggtg agttgtaata
     2041 taatctctgg tttctatggg tacttcctcc taataagatg aattaactat attatcttac
     2101 acaggacttg aagtttcaat cgttaacgtc tattactgaa ctcattcctt agagctaaac
     2161 taatgagaaa gagcaaaata actgatacaa aaagcaatcc tacaagaatt aatagactca
     2221 tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc
     2281 tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat
     2341 aaagcctttt acttaaaacc tttttttgta atcgtcaata aattttttcc ctttatcagt
     2401 taattgaaat cctatatcta aatagtcctc atatctaagt ttagtcagca tctcacttac
     2461 agatgctgga ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct
     2521 ttttgataat tcctgaattg ctagtaaata aattagtata gtagttgttt taatttgtac
     2581 catacctacc ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc
     2641 atcatcgcat agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc
     2701 gttgaataga taggaatatt ggtatctttt gatactgttt tcattggtct atcgtccaca
     2761 ctgctttcta cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt
     2821 atttgatctg gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca
     2881 ttatacgctt tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca
     2941 ttcggaattg aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata
     3001 tacttatcta gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt
     3061 ttttcaatat ttttagcgta aataataccg ttttctaaat ttaaccaagc atggggatct
     3121 tccttccctt tctcactctg accctctaag taaataacat ctatgccatc acttgctgca
     3181 aaatagtctt tgttttcctc tttgttcgca ttttttacta attttgtaaa ccaagcattt
     3241 cctccagttt ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga
     3301 acatcttcag gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt
     3361 aaatcaattt tatcttttgc tatattttca gtaatatctg ctaaaatcga attagtaact
     3421 actaccttta atttttcgtt cgaagccact gtatctttgt ctttactatt tgttgaacat
     3481 gccccgaata taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt
     3541 ctcatcataa ctttctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac
     3601 taattacaaa taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac
     3661 tataaccaat gaacaaacct agcaatgatg agcacgctcc gactgtcgcc gaaataaata
     3721 tcatagtttt taaactgttt gcatataaat atgctgtagc agctggtgta attaacattg
     3781 caacaattaa tatagttccc acactttgca tagctgttac tgctactaaa gttaatagaa
     3841 tcattaatag ataatgataa aaactgacat tcatccccat tgcttttgcc attaatggat
     3901 caaaagaagt gattaataat tctttaaaaa atagaacaac tgtaaccaac actaaaacag
     3961 aaacgccaat tgtaatccac ttatcaatat cttgtacagc taaaatattt ccaaataaga
     4021 tatgaaataa atccgttgag ctatttgcaa ctcctataag gataactcct aatgctaaaa
     4081 aagaactgaa agtaatacca attgctgtat ctccttttat tacactattg ctttttataa
     4141 aagtaataat gatagaagct aatagaccaa atacaatcgc accaataaaa aaattaatac
     4201 ctaaaatata agaaattgct actcctggca aaacagcatg agaaatagca tcacccataa
     4261 gagacattcc tcttaaaata ataaaacaac ctactgttcc agaaacaatc cctataacta
     4321 cagatgtaat tagagcattc tgtaaaaagt gaaagtcata cagaccatta ataaaatttt
     4381 gaagcatatt attccccttg tatattagag ttagggtttt aaccttccgg ttttgattgg
     4441 ataaccctat gaaccatcgt tattttaatt tggtattaac tcatttcttt tgagataata
     4501 tatttattgg attgagaggc taagccgttc ccccctctcc aataaatata gtatctccat
     4561 aggctttttt taaattctct tcattaaata cagattctac acttccgtga gcaattagat
     4621 ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca
     4681 caattagcac tgtttttccc tgatgtttca attcttttaa cgtatccata attattcgtt
     4741 cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag
     4801 caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat
     4861 gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg
     4921 catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt
     4981 ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca
     5041 ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga gggataatat
     5101 ttaacattgc tttaagcaat gtagattttc cagcaccatt tggaccaata atacctgtta
     5161 aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa
     5221 cattaagctt tttcacttct aacataactt tctccttcta aatgataatg attatcacga
     5281 tcatatggta tacaaaaatt atattttagt caaccctaaa ttttataatt tagatttttt
     5341 tctgttgttg aagattagga tgtcaaaatt atttgaaact actcgtatag taaatagtaa
     5401 gtaattttca aaacacgaag aaaaacaaaa agaaaaaagg aaacgccgaa gcatttcctt
     5461 tcagtgattt aatgagaaca acacgatttg acttagagtc tcaaaaagtc gaataggtac
     5521 actcatttaa gtgtctccat tcgacttttt ggggatgagc tggctattga ggtccgctta
     5581 ccctattgaa taaatacaag attgggtatt tctaataagt ccgcccttca tattggggtg
     5641 atcggggcga acatgccatc ttttcatata ccacctcctc gacactcaat aatcaaagat
     5701 tctcgttaat cttcttcgat gcttgcaatg atgttttcaa tattcatata acgaaaatct
     5761 tcttgtttga tataaacgat ctgagcggta catttttcct ttttgatgat ttcttttact
     5821 ttttcctcag aacgtgcgac ctgcggcgtt aaaagtaaaa cgtcatagtc tttataattc
     5881 agtttttctt caacgtttcg gatcgttgca taatctatct catacgatag atttttggct
     5941 ctgatcgttt caagcatttt tgatacgagc agcgacgcac tgattccggt ctcgcaaata
     6001 atcaatagat ccttatgcat aataccctcc tgtttttagc ctgcaaagcc aattggtgaa
     6061 aatgccacat aagttaaaat aacaatgact accaatgcta tagcagtcac cttggtgtat
     6121 ttccaaggct cgatatcgac ctttgtatcg aatgagttca acacaaattc tccatcaggt
     6181 ttcaatttgg aaaccaccat caaaatgata atgtccagaa cgaataatgc cgtcaaaaca
     6241 taaaggaaat ggatattttg gaaaagcatt tttgatagtg catacaagac gatatgcacg
     6301 gcaatcgtcg tttttgcccc aaaagcagtt gctttttttg aataaaaggc aaagagaatg
     6361 atcacgagca gcggcatgtt gtatagtcca ttgaattctt gaacgaactg atacaatcca
     6421 gccggtgcaa aagaaatgaa tggcgctacg ataatggaga taatccccac aacgaccgtt
     6481 actgttttac cagcacgagc gatttgctta tcatttgctt tcttattgat aagcggttta
     6541 taaaaatcca gtgaaaacaa ggtcgatgtc gcgttcaaag ctcccgcaaa tgatgataag
     6601 atcgcgccaa agataacagc ggcgaaaata ccaaagatca ttttaggcaa tacttcactg
     6661 actaaaaaag gatacgcgtt atctggattt gacattaaac tatcgccaaa aatattgcga
     6721 gcgacgatcc ctggaaagac aaggaataac gcacctaaga ttttgaagac gccgactagg
     6781 aaagcgccct tttgtgcctc agctaaattc ttgccggaca atgctttttg gacgatcatt
     6841 tgattggtac accagaaata cagattgtta aacagcatcc ctgtaaatag tgtcggccat
     6901 ggaacaacag ccgagtcgat cgatcccaca gagttaagca gccatggcgt gtttaccaca
     6961 acgtgttcga tcccgccaac aaagctgccg tcaccaagct tcattaagcc taaaattggg
     7021 accagcagac cacaagccag cagaccgact ccataaatgg agtcactatt ggcacttaac
     7081 gacaagccgc cgatcagcag atagaggatg ccgacaatac caattgccat cgctacaatt
     7141 atgatcgcga cgatcggctg gacgcccagc agctcatcaa tatggaagat cttattgaac
     7201 accagcgatc cagaataaag aaccacgggt aagaaagaag tcatataggt cacaataaat
     7261 aagatcgaaa taattcgttt ggtaaccgta tcataacgaa tctcgatgaa atctgagatt
     7321 gtattgatgc cgtatttaaa atattttggc aagaatatca cagccaaagc tacgatcgca
     7381 atcgtagcag ttacttccca ggccattact tccatccctg catgataact ttgaccattt
     7441 tgtccgacta tttgttcggt ggacaggttc gtcatgatga tcgtgccggc gattggcaaa
     7501 gcagttaagc tccgccctcc catgaagaac ccttctgatc cagaagtatc gacacttctg
     7561 gatctagaat aggcaaataa ccatacacag atgacaatca ctaggaaact gattatagaa
     7621 aaaagattca tcgtctagct ccttttcaat agattaaaga ccggtgacct ctcgaatgta
     7681 gcttctggct ttgactgcat attcaaatgg attggcttta gccgggtcct gttctgcttc
     7741 aactacgatc cagccttcat agtcgctggc atggattgct gtccagattg gagtgaaatc
     7801 aatcatccca tcaccaggaa ccgtgaagac gcctttcttc acgccgttta agaagctgtc
     7861 tttttccttt tctacttctt cagccgtttc ttgacgaata tctttgaaat ggatgtgttt
     7921 gatacgatct tgatgacggt tgtaaaccgc gattggattt tcaccagaaa agaccaaatg
     7981 tcccgtatcg aacaataatt taactttgtc tggatcagtc atttccatta aacgatcgat
     8041 ttcttccgtc gtttgaacgc cggtacccat gtgatgatgg taaacgatct ccatatcctt
     8101 ttcgtgggcc aaatccccta atttttctaa tccttccgcc agttttttcc actcttcatc
     8161 agtgaaaact ggtttatcat caaagactgg cgctgtcata atcccttgga tactatgacc
     8221 ctgctcagct actacgatga ctttagcgcc catcgcatgt aagaaatccc gatgtgcttt
     8281 gaaggcttcg gcagtttctt caaaaggttt agttgtcaga aacgcactga accatgcact
     8341 ggcaaccttt aagccgcgaa tattcaaata ttgttccaac acctctggat cttttggata
     8401 tttgttgcca atctctgttc cggtgaaccc agcaagggcc atttcactga tacattgttc
     8461 aaaggtattt tcactaccaa gctcgggcat atcatcattg gtccacgcga ttggtgcgat
     8521 ggctaattga atttgtttgg tcatattctc acactcctgc ttattttttt agttgtacta
     8581 aactcttcgt atcgaatgat tcttgacaag ccaacgcgat cttagttgat tgcaatccat
     8641 cttctgcatt aaccgctggt tgacgttttt cttgaataca tgagacaaat tcttgggctt
     8701 cacgaatgaa ggcttctctg aatcgttctg ggaagttttg tgaacatgga cggatgatcc
     8761 cttgatcgtt catgatcgtc acaagatttt tctcaggcgc ttgcgctacc cgcagcattc
     8821 ctttggttcc catgatctct gtttcgacat gatagccatg ggctgcgttt ctgccggcaa
     8881 ctagtaaagc tattgtttta tcgctcaggc tcatcattgc ggctccagtt tcaagctcac
     8941 cagcctgatc caattctgga tacgccgcat ttttcccaat cgcccacact tgatcgactt
     9001 cttggccggt aaaccagcga accaaatcaa tatcatggat cgccatatct aagaacaagc
     9061 cgccgctgtc gttattcatg gcaaatttga caaagctttc caagccttcg ctcggatcga
     9121 tcccataaca acgaatcagt gtgatttcgc cgatttcgcc atcttctacc attttcttcg
     9181 catactgata cgactcatca tagcggcgca tgaagcctaa catgaagacc tgttcaggat
     9241 gctcttcgat gactttttgg gtctttttaa tatcttcaac ttcaaggcct aatggttttt
     9301 cagtaaagac atgcttgcca ttttccatcg ccaagcggac ttggtcacag tgaaagcctg
     9361 atggtgaaac aataaagact gcatcgatca cggaactttt gaccatttct tcatagctgg
     9421 aaaaagtatg cttgacgcct aatttcttct gtgcgaatgc taattctgtt tcaactaaac
     9481 tgcacgcagc cgtaagctca caacaactga cacctgttgc taaattttgt gcatgtactt
     9541 ttcctaatct tcctaaaccg acgatgccta ctctaatgtt gttcatgaaa tacctccgtt
     9601 ttctgagaat tactcattgt atagtgctgg tcgttcatcc atttgaatca ttacttttgt
     9661 ttttgtttca cgagactcat gacaagccat catggtgatc gctgctacat acccatccca
     9721 agcggtagga ccgacaaccg catcattttt gatcgaatcg atccacagct tgaattcata
     9781 atcataggca tccaaaaagc gggtctgcca atctggtgaa acaggtgtgt aatttttttc
     9841 ttccgattta attcgtgtat aggctgggtc cgtcattcct acttctgccg tttcgaaaac
     9901 cacatcacat ttaatatcgt agccaaagcg gcaattcaca aagatttcca aatcaatcgt
     9961 tacaccggat tttgtttgaa agatcagcag ctgcggatca tgcaaatcgg catgggagtt
    10021 gctggttttt ttcggcaaga tcatttgaac agattcataa tcttcattta gcaaccagcg
    10081 aagcacatca atctcatgaa tcgcggtgtt gctgatcgcc atcggtgttg tgtagttttc
    10141 atctgcgcta ggatttctat gggcacaatg catcattaat gcttctccgt attgcttcga
    10201 ttcgattgct tctttcagtt caatgtagcc gcggtcatag cggcgcatga agccaacctg
    10261 aaccaatttc ttcccataag caacttccgc atcaacgatc cggcggcagc ctgctgcatc
    10321 catcgctaat ggcttttcgc agaaaacata tttcccatgc ttgattgctt ctaacacata
    10381 ctgctcatgg gtcgggtccc aagaagtcac aacaacgaca tccacatctt cagaagcgat
    10441 caatgcttct ccggtttcaa agaatttagc atcgacacgt ttggcaacct ctttagcagc
    10501 ttttacattg atatctgaga ctgctgtcac ataagcccct tgtgttttct gattgatcct
    10561 gtcgatatgc tcgatgccga ttgcacctgt tccgataacg cctactttta ctctcaatta
    10621 cttatccccc tgttctgttg tttaagcaca catagtatgc gcttacaaaa ttattcctgt
    10681 gtagctttta ttcttaaaag gtagagccct ctctttgctc cgccttttac tacccataag
    10741 cttgtacgta taattcctta atttgactat gactaggcac ctttttatta ttttgcggac
    10801 ttccgctggt caacgcatcc gcggccattt tgtctaaggc tgccataaac gcttgctgat
    10861 caataccata atctcgaatc gttgggatcg acagctgctc acagagctct tggatttttt
    10921 taatcagata atccgcagcc tcttccagcg ataaatcttc ggctgccgga taaataaatc
    10981 tggcgatcgt cgctaactca gtctgaatag catcctttga gaattcaaaa aagaccggca
    11041 ataacatcgc atttgatatc ccatgaggaa tgtgaaaaag tgcccccacc ggtcgactca
    11101 ttccatgaat caatgtgact gacgcattcg aaaaggcaat ccccgcctcc gttgaagcta
    11161 agctcatttg ttctctggct tcagtgtcgt cgctattttc atatgcctga gaaagcgtgt
    11221 tcaaaatttt cttcatcgct tccagtgaaa atagttgcgt tacttcattg gctttgactg
    11281 aaacataaga ttccaaagcg tgacatagcg catcgactcc cgttgccgcg gttacttttg
    11341 gtggtacagt tttcgtaaaa tcagagtcaa cgattgagat ctgtggcatg aaaacgagat
    11401 ccttcatcat cattttgact gctgtttttg tattggtaat aactgccaca tcggttactt
    11461 ccgaaccagt tccagcagtt gttgacactg caatcagcgg aatgcccgct tgcagctgat
    11521 caaaatttct tgcatgaatc gcttcgagac ttaattgatt agcagccaaa acggctacca
    11581 ccttggctgt atccagacag ctgccgccac caaaagcaat gatatgatca cattgatgct
    11641 tctgaaaaag cttcagcgca tcataaacat tttcttgcgt cggttcggct tgaatgtctg
    11701 tatactccgc taattgatca tctgtttttc ccaacagctc cttgatcttt tgaagatgcc
    11761 cggtctgttc cataaccgta tcactaatga ttaacggctt gttgcctagt tgttctgatt
    11821 cttccataag aaattggatt gttccgccgc caattcttaa attggcaggc attttcagtt
    11881 gaatattcac agaaaaaacc tctctctggc ttatagccta taacagaatc ctcgaagctc
    11941 tttaacagag cttcgaagca aatctttaaa tctcactttg cgcataacgt gctgtaacaa
    12001 ctttccgatg ggtataaaac tcaacactgt ctttgccatt ggcatgcaaa tcaccaaaga
    12061 aagagtgctt ccagcctgaa aaggcaaacc aagccatcgg tgcaggcacc ccaagattga
    12121 cccccagcat cccagcatca acattttctc tgaagtagcg aatcgctgat gcgttgttgg
    12181 tatacagaca cgaaccattc gcaaattcag attcattcgt cagctcaacg gctgccatca
    12241 aatcttttac ccgcacgatt gatgcgaccg gggcaaaaat ctcatcctgc cagatcttca
    12301 tatccatcgt gacattatca aaaatcgttg gtccaacaaa atagcctttt tctggaatgt
    12361 tttcgcgacc atctagtacc agtttcgctc cttcatcgac ccctttttgg atatagtcaa
    12421 aggtccgctg ctgtgcatct tttcgaatca ctggtcctaa aaagacccct tcgtccaagc
    12481 cattgccgat cttgatcgct tttgccgcag ccttaaattt ttcaacaaac tgatccgcga
    12541 tttcctcttc caaaacaatg acggaggctg ccatgcagcg ttcaccagca gaaccaaaag
    12601 ctccgccaat aaggtccttg actgcccgat caatattggc atctttcagc acaacaacat
    12661 gatttttcgc tccagtcaac gcttgtaccc gttttagatt ttttgttcct tcacggtaaa
    12721 cgtattcacc gacatttttt gatcctacaa agctgatgcc ttttaccagt ggatcttgca
    12781 gcaatgtgtt gacaacatcg tgggcaccat agacaacatt aaaaacaccg ctaggaaagc
    12841 ctgctttttc aaccagctca acaattttct ccatcaacaa tggtgttttt tctgaaggct
    12901 tcagcaccac cgtatttcct gccgccagag ccatcgggaa gatccagaag ggcaccatca
    12961 ttggaaaatt aaacggtgtg atccctccga ttactccgat tgggtaacga taattcgtga
    13021 tctcaatatc agtcgccgct gttgataaag aatctcccat cattaaattg gtgattcccg
    13081 ctgcgtgctc cacattttcg attcctcgtt gaacttctcc cagtgactcg gacaatgatt
    13141 tcccattttc cagcgtgatc aaacgcgcca attcactttt atgatctgtt aaaagctgat
    13201 gcagcttgaa cagataacgt ccgcgaacag gaacaggaac ttttttccaa gtttgaaacg
    13261 cagcatcggc agctttaact gcttcggctg tctctgcttt gctagacaac ggtacattcg
    13321 ccagcgcttc ttctgtagcc ggattcatta cttcttcaaa ctctgttgag cgactagcga
    13381 cccaacgacc atcaatataa tttcttaaaa atcttgtcat tta
//
LOCUS       DAZKSR010000085        13379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000085 DAZKSR010000000
VERSION     DAZKSR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.085"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8XG_002231"
     CDS             4..2376
                     /locus_tag="ACG8XG_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0774125.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8XG_002232"
     CDS             2437..3897
                     /locus_tag="ACG8XG_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0774126.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8XG_002233"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8XG_002233"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0774127.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG8XG_002234"
     CDS             6128..6277
                     /locus_tag="ACG8XG_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774128.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG8XG_002235"
     CDS             6352..6945
                     /locus_tag="ACG8XG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0774129.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG8XG_002236"
     CDS             6958..7857
                     /locus_tag="ACG8XG_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0774130.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8XG_002237"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8XG_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0774131.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG8XG_002238"
     CDS             8723..8983
                     /locus_tag="ACG8XG_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774132.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG8XG_002239"
     CDS             9428..9634
                     /locus_tag="ACG8XG_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0774133.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG8XG_002240"
     CDS             9655..9885
                     /locus_tag="ACG8XG_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774134.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG8XG_002241"
     CDS             9901..10338
                     /locus_tag="ACG8XG_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774135.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG8XG_002242"
     CDS             10331..11038
                     /locus_tag="ACG8XG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774136.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG8XG_002243"
     CDS             11202..11402
                     /locus_tag="ACG8XG_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774137.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG8XG_002244"
     CDS             11418..11597
                     /locus_tag="ACG8XG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774138.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG8XG_002245"
     CDS             11922..12185
                     /locus_tag="ACG8XG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0774139.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG8XG_002246"
     CDS             12179..12529
                     /locus_tag="ACG8XG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0774140.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG8XG_002247"
     CDS             complement(12553..13167)
                     /locus_tag="ACG8XG_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774141.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4951 a         2206 c         2745 g         3477 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgaca
//
LOCUS       DAZKSR010000086        13211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000086 DAZKSR010000000
VERSION     DAZKSR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.086"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..215)
                     /locus_tag="ACG8XG_002248"
     tRNA            complement(143..215)
                     /locus_tag="ACG8XG_002248"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Lys,seq:ctt)
     gene            596..1165
                     /locus_tag="ACG8XG_002249"
     CDS             596..1165
                     /locus_tag="ACG8XG_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0774142.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1152..2012
                     /locus_tag="ACG8XG_002250"
     CDS             1152..2012
                     /locus_tag="ACG8XG_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0774143.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2202..2906
                     /gene="yycF"
                     /locus_tag="ACG8XG_002251"
     CDS             2202..2906
                     /gene="yycF"
                     /locus_tag="ACG8XG_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0774144.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2911..4746
                     /gene="walK"
                     /locus_tag="ACG8XG_002252"
     CDS             2911..4746
                     /gene="walK"
                     /locus_tag="ACG8XG_002252"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0774145.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4743..6059
                     /gene="yycH"
                     /locus_tag="ACG8XG_002253"
     CDS             4743..6059
                     /gene="yycH"
                     /locus_tag="ACG8XG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0774146.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6060..6929
                     /locus_tag="ACG8XG_002254"
     CDS             6060..6929
                     /locus_tag="ACG8XG_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0774147.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6984..7793
                     /locus_tag="ACG8XG_002255"
     CDS             6984..7793
                     /locus_tag="ACG8XG_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0774148.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7896..8525)
                     /locus_tag="ACG8XG_002256"
     CDS             complement(7896..8525)
                     /locus_tag="ACG8XG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0774149.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8695..9987
                     /locus_tag="ACG8XG_002257"
     CDS             8695..9987
                     /locus_tag="ACG8XG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0774150.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10063..10725)
                     /locus_tag="ACG8XG_002258"
     CDS             complement(10063..10725)
                     /locus_tag="ACG8XG_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0774151.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10950..11234
                     /gene="groES"
                     /locus_tag="ACG8XG_002259"
     CDS             10950..11234
                     /gene="groES"
                     /locus_tag="ACG8XG_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0774152.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11285..12910
                     /gene="groL"
                     /locus_tag="ACG8XG_002260"
     CDS             11285..12910
                     /gene="groL"
                     /locus_tag="ACG8XG_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0774153.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4391 a         2273 c         2843 g         3704 t
ORIGIN      
        1 ttacaatttg agtgtaacat aaatggcttt tttatttgtc tcgcttaatt atacaaacgg
       61 cgaaaataaa aaaagaacag cgtaaatgtt ttcgaaatgc tttcgcacca acatttacct
      121 gttccgtggg tttggcaccc aatgggccgt gaggggctcg aacccgcgac ccgctgatta
      181 agagtcagct gctctaccaa ctgagctaac ggcccgtaca aaaagtactt aataatagta
      241 gcactaagaa ttttaaaatg caagactaca tttaaagaaa atcagaaaaa tctttacttc
      301 cttataggaa atcattcata gattattcac atttgctcgc tattatataa gcataccaac
      361 aaacaaagtt cttgcaagag aacaaaccct aacacttttt tcatttttta ctcctttccc
      421 gccgtctccc cggcgggtct cctgttctta acgataaaag acaagaaggc ttagccttct
      481 tgttttttgt tttcatggaa tcccgctaat caaatcacct agttaaagca actagattga
      541 atgtttatca taagtctgat atcatgttta tgaaatgaaa aaaggagcgg ttatgatgga
      601 aaaaatgcgt cagcaattaa tggagtgggg gaaggaacta gaaacattcc gtctgcctca
      661 ctgggaagaa ctccctgact tagaacttta tatggaccaa gtcagaacgt tagttgatcg
      721 ttatctttca cctgtgatac aaggagaaaa gcatccattg cttacctctt ctatggtaaa
      781 caattacgtt aaactaggtt tgataccagc accagttaaa aaaagatata acaaagaaca
      841 cgtagcattt ttacttgcaa tcactacgtt gaaacaagta ttgactattc ctgaaatcaa
      901 agaagggata ttgttccaag gaaaaacagt aggaattcgt gaagcctata atctattttg
      961 cgatgaacaa gaagcagctg tgtggatggt cagccaatta gcacaaggta aatctcatcc
     1021 gcaaaaattc gaccaggcaa cccctgtaga atatattgct gtaagagcgg caactttatc
     1081 gtttgcaatg aaacttcttg cggaaaaaac gattgttcta gaaaccgaat atttaaaaga
     1141 ggagaaaaca aatgaaaaac aataaaatcg caattttagt agattcaggt acggatgtgc
     1201 cagaagaatt gataaaaaaa tacgagatgt atatgatccc attgaaaatt atttataaag
     1261 atcaggtata tacagataaa attgacatta cctctgaaga gatctatcaa cgtttgccag
     1321 aggaaatccc tggtacatct ttgccggatg gtgagtcgat cacagaaatc tttgagcaaa
     1381 tcaaaaaaga cggttatgaa aaggtacttg cggtaacaat ttccagtgga ttgagcggga
     1441 catataatgt ggttcgttta attgcacaac agcaagaaga attggaagca tatgtactag
     1501 atacgaaaaa tattggtatt ggtgcaggtt tttctgcgat ccaagcagca aaatggctgg
     1561 aagaaggagt agaatgggat caactgatca gtaatctgaa tgagttagtc gaaagaacaa
     1621 aagttttctt taatgttgcg acattggaat atcttcaaaa aggtggacgg atcggtttag
     1681 ttgcttctat tgtaggaacc gctttaaagc ttaacccaat catctcatgt aatgaagaag
     1741 ggatttatta cacagtagga aaagctaggg gacgtaaaaa aagtttggat aaaacggttg
     1801 cttacgtcaa agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt gcccatgggg
     1861 atgcgaaaga agaagcaatt gaaatggaag cgagacttaa aagagagttc ccacaagcaa
     1921 accaaatcta ctttggccag atttctccgg cattagtagt ccacactggt ccgggattat
     1981 tgggtgtcgg tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac tgaggtgaaa
     2041 atcagtgttt tcctttttta ttgaagttag aacaaaaaat aataaagtat tttgcacttt
     2101 cattttaatt ttctcttttg ttttataggc aaagatgaaa caaaagcaga aatacggtat
     2161 aatgagaagg tatgacaata atatataaga ggtggctctt tatgaaaaaa gttttagttg
     2221 tcgatgatga gaagccgatt tcggatatcg tcaaattcaa tttagccaaa gaaggctatg
     2281 atgtttatac tgcatatgat ggggaagaag ccttggaaaa agttgctgag gtagaacctg
     2341 atttgatttt acttgatttg atgctaccta aaatggacgg cttagaagtt gctcgtgagg
     2401 tacgcaaaac ttatgatatg ccgatcatta tggtaacagc caaagattct gaaattgata
     2461 aagtcctagg cttagagtta ggcgccgatg attacgtgac caagccattt tccaaccgag
     2521 aattagttgc acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg aaggaaccag
     2581 aagaggcagc acctgcggaa ttgacgattg gtgatttgac gatccatcca gaagcttaca
     2641 tggtgacgaa acgaggagaa acaatcgagt taacacatcg tgaattcgaa ctactctttt
     2701 acttggcgaa gcaccttgga caagtgatga caagagaaca tttattacaa acggtttggg
     2761 gttatgatta ttttggagat gtacgaacag tggatgtaac tgtacgtcgt ttgcgtgaaa
     2821 agatcgaaga taatccaagc catccaaatt atttagtgac acgtcgtggt gtcggttact
     2881 atttgcgtaa cccagaacag gagtaattgg atgaaaagaa aagtccgctt ttttcgatca
     2941 gtcaatttta agatcgctat tacctttatt ctgattttat tgatctcgat cgaaattatt
     3001 ggtgcctact ttatacgtgg tttagaacgt tccacgatca atacatttat taaagatatg
     3061 aatcaaacgg tggaatcatt agcaacaaca atcagcccgg aattgaatcg aaaagataat
     3121 gcagatgatg aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag tgcgactagc
     3181 gatatcatcg aaatacgggt cgttgacgaa aaagggatca tccgaggaac aaccgatgtc
     3241 aatgaacaaa gcgctgtggg aaagaaaaat gattatgttg atatcaatga tttcacgact
     3301 aaacgttatg ttgctttaga taacgacgat aaacgggtga atatcaatgt tcaaccgatc
     3361 ctgtccccga ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa tatcgaacaa
     3421 aaatattcgg aaatcaataa cacagcagtc attttcttta ctgcctcact aattgcggca
     3481 tttatttcaa tgatcgtatc tgttttagtt gctcgttcga ttacacagcc gatcggtgaa
     3541 atgagagaac aagccattcg aatcgctaga ggagactaca gtcggaaagt caaagtgcat
     3601 ggacaagatg aacttggaca gctggctgat accttcaatc agttagctga acgtattgaa
     3661 gaagctcaag atacaatgga agctgaacgc aatcgtttgg atagcgtgct atcccatatg
     3721 acagacggcg tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa tgatatggct
     3781 gtttccttgc tagatatcaa aaacgaggaa gcaatcggac aatcgatttt gacactttta
     3841 gatatcgaag aagaatatac gttgcggaaa ttattagaag atccaaacga aatggtactt
     3901 gaccgttcaa ccagtacatt ggaaagtgat cagatgatct tgcgagtaga cttttctatg
     3961 atccgtcgcg aatcaggttt tatcagcgga ttagtagctg tcttgcatga tgtgacagaa
     4021 caagaaaaaa cagaacggga acgtcgtgaa ttcgtctcta atgtttccca tgaacttcgt
     4081 acaccgttga caagtatgcg tagttatatc gaagcattga gcgaaggtgc gtggaaagac
     4141 gaggaagtag caccaaattt tttgaaggta acgctagatg aaacagaccg aatgattcga
     4201 atgatcaatg acctcttaaa tctatctcgc atggatacag gtaataccca gcttcaatta
     4261 gaatatgtca atttcaacga gttagtgaat tttgttcttg accggtttga tatgatggtg
     4321 ggaaatcagg aaaaaaatta taagatccgt cgtgaattta cgcaaagaga tctttgggtc
     4381 gaagtagata cagataaaat catccaagta gtcgataata tcatgaataa cgcgatcaag
     4441 tattcaccag atgggggaac gattacttgt cgcttattag aaacgcataa taacgtggtt
     4501 ttaagtatca ccgatcaagg attggggatt cctaaaaaag atctgaaccg agtgttcgag
     4561 cggttctatc gagtagataa agcacgtgca agagcacaag gtgggacagg acttggatta
     4621 gctatttcta gagaagtgat taaggcgcat cgaggcgcta tttgggcaga gagcaaggaa
     4681 ggaaaagggt cgacttttta tatctcgctg ccatatgaac cttatgagga ggaatggtgg
     4741 gaatgagaat ctctgaaaaa gtcgttcgta taggactcat tttgatggtt gcgttaagca
     4801 tatacttctc ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc aacgtagatg
     4861 agacaaacac tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa gtctttcttc
     4921 cgcttcatgt tacatggcac cattcgggaa tgatccaaga aaccaacagt gaaaatctcg
     4981 tgtaccgatt gcaattgatc atcgataaag caagatttgg aaagctttct gaagtagttt
     5041 caggagacaa atcacagttt gaacaggtga aaaatctgaa ctctggtttt gagttggcct
     5101 ataatgcgcc ttttttgcta agagaataca aggaagcatt tgatttagat ttagatgtgt
     5161 cgaacttggc aaaagaaagc gaaaatatgt attttactag aatacaattc gatcaaggaa
     5221 ataaaaaaat ccgatttttg aattattcga accatctcgt atatgaagca aatatttcgt
     5281 tgaattggac agatatcaat aaagaattaa cagcaaaaga cacaaaatgg acagaaatga
     5341 caacagaacc atcgacgatc gacacgcaat atgctacgaa acattcgatc aaactgaaaa
     5401 agtatagtta tatcctgtca cagcaaccct atacactttt tagaaatgct tttttccaga
     5461 aaccagatag cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac ggtgggaatg
     5521 aaacaatgtc tatccattcg gaaacgcaag tagttgattt tcgtggagag ctgccagaga
     5581 aaaaagaaaa tgctagtgta ttttctgaaa gtttccccta tattagcaaa ttaggcagtt
     5641 ctttagggaa cctcagatat tttgaccgca gtgacaatac gatcgattat cggatattcg
     5701 tggaaggctt cccggttttt gggacagaca gcagaggaca gattggtgtg gaagttggcg
     5761 gtgaaacgaa tggttctgtg caagtgaata tccaaaccag tttgaatacg attcaagtgc
     5821 cgataccttc agacgaagaa gtagaacttg caagtacgaa tgaaatcatc cagcagttaa
     5881 aagctaaggg agcagatagc aacaagattg gttcagtgat cgttggctat acatggcaaa
     5941 atgtcaaaga aacgaatcgt gtcgttgatc tcttacctga atggtatgtg aaatataaag
     6001 ataactggta ttcagctaac gaccttctgg aaaaattacc tggattggag gcgaattaat
     6061 tggatttcaa aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg aatgtgtttt
     6121 tattcagcag ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc tctaatcaga
     6181 ctgaatcaat ccaacaacgt ctggctagcg acgatatcac ttacacgggg aagatttcgt
     6241 ctgagaacaa gcaaggctat tatcttagcg cggagcagac caatcttagc acttcattag
     6301 cgaattatcg caaggctggt aaaagcggct tgctggcaag tggtgtgttt ttagacggtg
     6361 atgttttgac gaaatcttta tccagttcag atgctgaaaa gaacgtgatc gatccagata
     6421 aaattagtag ttccttggaa gattttttat ctaacaaaga caatatttta ttcggtaatg
     6481 attatgtgta cacgaaaaac ttttctcatt tagaagaaga accactagaa atccaagcaa
     6541 gccaagagta tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt ctttctttag
     6601 aaaaaaacga taatgattat agcatcacga aatacatgca gacgcattta tctgatatcg
     6661 agcagctgcg cgaaaagacg gaactgtata cggaagagga agcaatcaat acattgtaca
     6721 tcaataacaa aataccaaga ggatcaaaaa ttttatggcg tcagctagct tattcctgta
     6781 ttctgaaagt ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg atcgaaacaa
     6841 gcgataaagt ggtccaaatt gagagtgtca acgctttcag caatacgatc gtgacaaaca
     6901 atactgtacc aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa agtttacaaa
     6961 gtaagaaggg aagtaggagt caaatggctg aagaaaaacc agcttttaaa atcagtatcc
     7021 tagcaagtgg aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa cggcttttgg
     7081 tcgatgcagg acttagcggt aaaaaaatca cttccttgat ggctcaaatt gatcggacac
     7141 cggaccaatt agatggaata cttgtgacgc atgaacatcg agatcatata catggcgtgg
     7201 gtgtcttggc tcgtaagtat catttggatg tttttgccaa tcagcagact tgggaagcga
     7261 tggaaccact gattggaaat gtaccagtgg aacaaaaaca tctatttgag atggggaaag
     7321 tcagaacatt cggcgatatc gatattgaga gtttcggcgt ctcccatgac gcagcggctc
     7381 cgcaatttta tcgtttccat aaagaaaatc ggtcattcgt catgttgacg gacaccggat
     7441 attgcagtga ttatatgcga ggtgtcattg aaaatgccga tggttatttg atggaaagta
     7501 atcatgatct tgagatgtta cgcatgggac cttatccgtg gaatttaaaa cagcgtattt
     7561 taggagatag aggacatctt tccaacgaag acggtgcact tgtcatgact gatgtgatcg
     7621 gtaatcggac caaacgaatc tatcttggcc atcttagcaa ggaaaacaat atgaaagaac
     7681 ttgctcattt aacaatggaa aatgttttga aagaaaaaga ttttggcgtt ggacatgatt
     7741 ttgaaatcta tgatacagat ccagatgaag caactgagct attttcgatt taaacagttc
     7801 aatcaataaa aatataaaaa gcagaagata aaatcaatca gggttggttt tatcttctat
     7861 ttttattttt ttagcatatt aaaaaaataa ccgtattatc gactgactac actgatgtgc
     7921 tgcttttcat gtttctcatc cagtatctca tctactaatg caatcgcgta atctgccatg
     7981 ctgatcgtac tgtcgccagc agcgtttctt tgtaaataat catcacttaa caaataagta
     8041 cctgtacgtt tgccatgtgg atcaaataaa gaagctggac tgatataagt ccaatgtaga
     8101 tttttagatt gtttcaattt taacaatcct tcgtacatat tgaaagctgt tggatagaag
     8161 ggtgcatttt taggtacttg gtcaatcata cgagtcgtct tttctgtatc taagaataac
     8221 gaagaggcac ccccaacgac aattaagcgt gtatcggttc cttcaagcat ctcaatcaaa
     8281 tgggacaaag aagtttgatg catttcttct tttcctttgg gagcgttgaa agcatcaacg
     8341 acaacatcaa aagatgcaag atcagtagtt gtcaactcaa ataaatcctt ttgtataaac
     8401 ggaacatctg atggcaattt ttctggatga cgcacgattg ccgtcacgtc taaatctctg
     8461 gacaatgctt cgtctaaaat aaatgaacct gcatggccag tagctccaat gagtgcaatt
     8521 ttcataaaaa acctcctttt ttattcctgt ccattgtaag cagttgctga atattcgtca
     8581 aagaatccga tttttgaaaa gctcagtgcg cattcctttc atggttcgtg atcattgggt
     8641 atagttaaat taagaaaact gattgataaa aatagaagtt gaaggagata agaaatgaaa
     8701 gagtggttag atcgtgcctt tttaccaatg ataacatctg ttgtgaatct tagaccctta
     8761 actgctttgc gtaatggaat gacttacacg atcccaataa ttattgttgg ctcgctgttt
     8821 ttcttatttg ctcattttcc tgtggattac atcgctcagt ggatagacaa aacaggattt
     8881 tctatgtatt ttacacaagt ataccaggct acatttgatg tgatagcctt ttggatcgtt
     8941 tttgccattg catacgctta tgcaaaagaa gaagacatcg atcgcttacc agtcggcata
     9001 acagccttgt ctgcttatct actgcttatt cagccactaa atactcagat agatactgtc
     9061 tggacaggtg ctgaagggat actaggagca gttacaactg ggctcatcat cggatggggc
     9121 tatgcacgtt tagtaagact tgcgaaaggg tggaagcaaa gagagaagat acctgaaaat
     9181 gtttggcagt cttttacttc attgattcca atcattgtta tagtcacttt tacgcttatc
     9241 ggttcagcgg tttttcaaca gttgttcaga atgactgttg ttcaaggagt atggacatat
     9301 gtccagcttc ccattcaatc gttcatagac acgttgtttg gtgtgctttt attaggtttt
     9361 tttgttccgt ttttatggtt atttggtctt catggatctg cgatggtcaa tgggttggtt
     9421 agtcccgtct tgcaagcgaa ttcattagcg aatgcagaaa tcttagcctc aggacaagag
     9481 ttgacggtgg caaatggcgg acatatcgtt actcagcaat ttctcgatca gttcatgacg
     9541 gttacgggtg ctggattgac gttaggggct gtatttttta tgacggtttt tgcgaaatcg
     9601 aagaaataca gagaattagg aaatcttgct cttttaccag cctttttcaa catcaatgaa
     9661 tcaattattt tttccacacc gatcgtgatg aacccgatga tggctgttcc ttttattttc
     9721 gcaccggtcg tttcaggatt gattacctat tccgctttat attttggatt cgttccttta
     9781 tttacagcag tccaagtccc ttggacaaca ccgccgatcc tctcgggttt cttagtaggc
     9841 ggagtgcctg cagcgatttt acaaggtatc gtgttgtcaa ttagtttttt catttatttc
     9901 ccatttttga aaaaaataga ttctgcaaac tgcaaaaagg aaagacagac aaaaaatgat
     9961 ggagcagcag aaaagatgaa cgactaaaaa acaaaaatac tcctgtcgct ttagattggg
    10021 aaaatcacca atctaggatg acaggagtat ttatagtcat gtttatacta gccctttttg
    10081 gacagctaat tgaacaagaa caactaatgt gttcattcct acgtgtgtga tcatcgatgt
    10141 ccagatccga ccagttgctt tgtactctag gctgaagaag aaacctagga aaaagtagat
    10201 tagcagatga ccatcattat gagcaaacgc aaacagcaaa gagctgatca ctgctccgac
    10261 ccaaaaattt gaatatcgac tgatgatccc cagtatactg cggcggaata cgaactcttc
    10321 catgatgggt ccagcaacag ttgtcgccaa aataaaagcg ggtgcttcca tgatcatctg
    10381 gatgatattt tgtgtattct cggaaggggt tgctttgcca aacaatacag attctaacat
    10441 gacagcgatg ctttgcagaa tcaatgcgac aaagattcca accaatccat aaataacaat
    10501 ctttggagca gaagcttttt ttaccgctga ttcgatcgct aagggttcac gttgcttaaa
    10561 ataaaaaata gctaggacaa cggcgcctag tatatacatg actgcagttg ctgtgatcaa
    10621 ttcagacgta gattttacaa gtcctatcga agctaggatc aagggtgaga taaaaattag
    10681 accatagcaa aaaatgctga ggaagctata tttttttaaa gacatgtaat tcctcctttt
    10741 aagggataac aagcatgcaa ggcaagctta tatcgattga tttcaattat atcataattt
    10801 caataagcag ctgtcagaat aaaatttctt ttctttgatt taaaagcttg caaaaaagag
    10861 ggagaatgag tattattaaa aatgtaggtt agcactcgaa ggttaagagt gctaacagat
    10921 taaaaaaaga gttatcggag ggatttatcg tgttaaaacc attaggtgat cgcgtcatta
    10981 tcgaagtcgc aaaagaagaa gaaaaaactg tcggaggaat cgttttagct tctgctgcta
    11041 aagagaaacc ccaaactggg acagttgttg cagtcggtga aggccgtttg ttagaaaacg
    11101 gtgaaaaagt tcctgccgct gtaaaagcag gcgaccaagt tatgttcgaa aaatacgccg
    11161 gtacagaagt aaaatacgaa ggaaaagaat atctgatcgt cgcaggaaaa gatatcatgg
    11221 cgattgttga gtaaatttgg aagataaaaa aataatcaaa aataatcaat gaggtgaatc
    11281 aatcatggca aaagaattga aatttgcaga agacgcacgc gcagctatgt tacgcggggt
    11341 agataaatta gcagatacag tgaaagtaac attaggacct aaaggccgaa atgtcgtact
    11401 agaaaaatct tacggctctc cattgatcac aaatgatggt gtgacgatcg caaaagaaat
    11461 cgaattagaa gatcattttg aaaacatggg tgcaaaactt gtatcagaag tagcatcaaa
    11521 aacaaatgat attgcaggag acggtacaac gacagcaact gttctgacac aagcaatcgt
    11581 tcgcgaaggt ttaaaaaacg taactgcagg tgctaatcct ctaggcattc gtcgcgggat
    11641 cgaattggca acaaaagcag cagtcgaaga attgcataat atctcaactg ttgttgattc
    11701 aaaagaagcc attgcacaag tagcagctgt ttcttcgggt tctgataaag ttggacattt
    11761 gattgctgat gcaatggaaa aagttggaaa cgacggtgtc atcacaatcg aagaatcaaa
    11821 agggatcgaa acagaactag atgttgtaga aggtatgcag tttgatcgtg gttacttatc
    11881 acaatatatg gtaacagata acgacaaaat ggaagctgtt ctagaaaacc catatatctt
    11941 gatcacagac aaaaaaatct ctaacatcca agatattctg ccattgttag aacaaatctt
    12001 gcaacaatca cgtccattgt tgattattgc tgacgatgta gatggtgaag cattgccaac
    12061 tcttgtattg aacaaaatcc gtggaacatt caacgtagta gctgtaaaag ctcctggttt
    12121 cggcgatcgt cgtaaagcaa tgcttgaaga tatcgctatt ttgacaggtg gtacagtaat
    12181 tactgacgat ctaggtttag aattgaaaga tgtaacaatc gaaaacttag gaaatgcttc
    12241 taaagttgtt gtagataaag acaatacaac gatcgtagaa ggttctggag aaaaagaagc
    12301 aatcgaagct cgtgtgcaat tgatcaaaaa ccaaattgct gaaacaactt ctgattttga
    12361 ccgcgaaaaa ttacaagaac gcttagctaa attagcaggt ggtgtagctg ttgttaaagt
    12421 tggtgcagct actgaaacag aattgaaaga attaaaatta cgcattgaag atgcattgaa
    12481 tgctacacgt gccgcagtag aagaaggaat ggtctcaggt ggtggtacag ctctagtgaa
    12541 cgttatcagc aaagtttctg ctgtagaagc agaaggagac gtggcaacag gtatcaagat
    12601 cgttgttcgt gctttagaag aaccaattcg tcaaatcgct gaaaacgctg gttatgaagg
    12661 atcagtgatt gttgataaat tgaagaacgt ggaactaggc actggtttca atgctgctac
    12721 tggagaatgg gtaaacatgg ttgaagcagg tatcgtagac ccaactaaag taacacgttc
    12781 tgccttgcaa aatgccgctt ccgtttctgc cttgttatta acgacagaag cagtcgttgc
    12841 agataaacca gaaccagcag cgccagcagc tcctgcaatg gatccatcaa tgatgggcgg
    12901 tatgatgtaa gaaaaagcaa cttgcttatc tacaactgat cattagtgaa agacagataa
    12961 aaagaggctg gaacagaagc gtttaactcc aagaaataag aagaaattca cggaaattgc
    13021 ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgctgtt
    13081 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttttg
    13141 tgtgtaatta taatgaaaat agttattttt aatagttata atattttatc tattaaaagt
    13201 aaatatccat t
//
LOCUS       DAZKSR010000087        12740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000087 DAZKSR010000000
VERSION     DAZKSR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.087"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..542
                     /locus_tag="ACG8XG_002261"
     CDS             99..542
                     /locus_tag="ACG8XG_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0774154.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="ACG8XG_002262"
     CDS             complement(548..1732)
                     /locus_tag="ACG8XG_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0774155.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="ACG8XG_002263"
     CDS             2000..2698
                     /locus_tag="ACG8XG_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0774156.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="ACG8XG_002264"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="ACG8XG_002264"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0774157.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="ACG8XG_002265"
     CDS             complement(3508..4041)
                     /locus_tag="ACG8XG_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774158.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="ACG8XG_002266"
     CDS             complement(4338..6719)
                     /locus_tag="ACG8XG_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0774159.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACG8XG_002267"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="ACG8XG_002267"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0774160.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="ACG8XG_002268"
     CDS             7420..7959
                     /locus_tag="ACG8XG_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0774161.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="ACG8XG_002269"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="ACG8XG_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0774162.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="ACG8XG_002270"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8XG_002270"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="ACG8XG_002271"
     CDS             9066..10232
                     /locus_tag="ACG8XG_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0774163.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="ACG8XG_002272"
     CDS             10278..11774
                     /locus_tag="ACG8XG_002272"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0774164.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="ACG8XG_002273"
     CDS             11855..12538
                     /locus_tag="ACG8XG_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0774165.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12547..>12740
                     /locus_tag="ACG8XG_002274"
     CDS             12547..>12740
                     /locus_tag="ACG8XG_002274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774166.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4272 a         2230 c         2631 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaaggta
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DAZKSR010000088        12731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000088 DAZKSR010000000
VERSION     DAZKSR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.088"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8XG_002275"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8XG_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0774167.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG8XG_002276"
     CDS             complement(322..891)
                     /locus_tag="ACG8XG_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0774168.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG8XG_002277"
     CDS             1077..1979
                     /locus_tag="ACG8XG_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0774169.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8XG_002278"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8XG_002278"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0774170.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8XG_002279"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8XG_002279"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0774171.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8XG_002280"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8XG_002280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0774172.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="ACG8XG_002281"
     CDS             8546..8731
                     /locus_tag="ACG8XG_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0774173.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="ACG8XG_002282"
     CDS             complement(8793..9989)
                     /locus_tag="ACG8XG_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0774174.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="ACG8XG_002283"
     CDS             complement(10159..11595)
                     /locus_tag="ACG8XG_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0774175.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSFIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="ACG8XG_002284"
     CDS             11888..12697
                     /locus_tag="ACG8XG_002284"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0774176.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgaa acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcgctacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg a
//
LOCUS       DAZKSR010000089        12671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000089 DAZKSR010000000
VERSION     DAZKSR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.089"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8XG_002285"
     CDS             6..305
                     /locus_tag="ACG8XG_002285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0774177.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8XG_002286"
     CDS             378..1739
                     /locus_tag="ACG8XG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0774178.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8XG_002287"
     CDS             1761..2021
                     /locus_tag="ACG8XG_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774179.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8XG_002288"
     CDS             2035..2787
                     /locus_tag="ACG8XG_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0774180.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8XG_002289"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8XG_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0774181.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8XG_002290"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8XG_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0774182.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8XG_002291"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8XG_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0774183.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8XG_002292"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8XG_002292"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0774184.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8XG_002293"
     CDS             7161..8558
                     /locus_tag="ACG8XG_002293"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0774185.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8XG_002294"
     CDS             8639..9079
                     /locus_tag="ACG8XG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0774186.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8XG_002295"
     CDS             9227..9553
                     /locus_tag="ACG8XG_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0774187.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8XG_002296"
     CDS             9554..10303
                     /locus_tag="ACG8XG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0774188.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8XG_002297"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8XG_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0774189.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8XG_002298"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8XG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774190.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8XG_002299"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8XG_002299"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0774191.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2588 g         3854 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag t
//
LOCUS       DAZKSR010000090        12432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000090 DAZKSR010000000
VERSION     DAZKSR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.091"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACG8XG_002300"
     CDS             complement(<1..190)
                     /locus_tag="ACG8XG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774192.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="ACG8XG_002301"
     CDS             complement(200..1015)
                     /locus_tag="ACG8XG_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0774193.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="ACG8XG_002302"
     CDS             complement(1277..1720)
                     /locus_tag="ACG8XG_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0774194.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="ACG8XG_002303"
     CDS             complement(1854..2192)
                     /locus_tag="ACG8XG_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0774195.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="ACG8XG_002304"
     CDS             complement(2180..2557)
                     /locus_tag="ACG8XG_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0774196.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="ACG8XG_002305"
     CDS             2781..3974
                     /locus_tag="ACG8XG_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0774197.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="ACG8XG_002306"
     CDS             complement(4140..4562)
                     /locus_tag="ACG8XG_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774198.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="ACG8XG_002307"
     CDS             4957..5151
                     /locus_tag="ACG8XG_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774199.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="ACG8XG_002308"
     CDS             5148..5594
                     /locus_tag="ACG8XG_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774200.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="ACG8XG_002309"
     CDS             complement(5804..6976)
                     /locus_tag="ACG8XG_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0774201.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="ACG8XG_002310"
     CDS             complement(7182..7997)
                     /locus_tag="ACG8XG_002310"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0774202.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="ACG8XG_002311"
     CDS             complement(8147..8593)
                     /locus_tag="ACG8XG_002311"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0774203.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9105)
                     /locus_tag="ACG8XG_002312"
     CDS             complement(8590..9105)
                     /locus_tag="ACG8XG_002312"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0774204.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9178..10092)
                     /locus_tag="ACG8XG_002313"
                     /pseudo
     CDS             complement(9178..10092)
                     /locus_tag="ACG8XG_002313"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10089..12416)
                     /locus_tag="ACG8XG_002314"
                     /pseudo
     CDS             complement(10089..12416)
                     /locus_tag="ACG8XG_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
BASE COUNT         3514 a         2429 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctatgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DAZKSR010000091        12279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000091 DAZKSR010000000
VERSION     DAZKSR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.092"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..2156)
                     /locus_tag="ACG8XG_002315"
     CDS             complement(165..2156)
                     /locus_tag="ACG8XG_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0774205.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(2300..2566)
                     /locus_tag="ACG8XG_002316"
     CDS             complement(2300..2566)
                     /locus_tag="ACG8XG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0774206.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(3076..5202)
                     /gene="pnp"
                     /locus_tag="ACG8XG_002317"
     CDS             complement(3076..5202)
                     /gene="pnp"
                     /locus_tag="ACG8XG_002317"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0774207.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(5390..5659)
                     /gene="rpsO"
                     /locus_tag="ACG8XG_002318"
     CDS             complement(5390..5659)
                     /gene="rpsO"
                     /locus_tag="ACG8XG_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0774208.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            5910..6473
                     /gene="def"
                     /locus_tag="ACG8XG_002319"
     CDS             5910..6473
                     /gene="def"
                     /locus_tag="ACG8XG_002319"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0774209.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(6726..7247)
                     /locus_tag="ACG8XG_002320"
     CDS             complement(6726..7247)
                     /locus_tag="ACG8XG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0774210.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(7250..8092)
                     /locus_tag="ACG8XG_002321"
     CDS             complement(7250..8092)
                     /locus_tag="ACG8XG_002321"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0774211.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(8085..8810)
                     /locus_tag="ACG8XG_002322"
     CDS             complement(8085..8810)
                     /locus_tag="ACG8XG_002322"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0774212.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            8986..9282
                     /locus_tag="ACG8XG_002323"
     CDS             8986..9282
                     /locus_tag="ACG8XG_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774213.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      9538..9712
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            9850..11442
                     /locus_tag="ACG8XG_002324"
     CDS             9850..11442
                     /locus_tag="ACG8XG_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0774214.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            11455..12195
                     /locus_tag="ACG8XG_002325"
     CDS             11455..12195
                     /locus_tag="ACG8XG_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774215.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         3515 a         2552 c         2389 g         3823 t
ORIGIN      
        1 ttcatattta ggaataaaaa aggagatgcg gcatttttca gccacatctc cttatcactt
       61 caaaatcatt gcgttacttg ttgctaaaca gctttttcat gacctctgat aaacggtgtt
      121 caagtagctg acattcgaca ttagttcagg tcaaagcgct actttcacaa ccgctctttc
      181 aaccatgttt tccgagtatt tccaatgata agcggaacag tttcatgttt tacttcgctg
      241 tactctagcc caaaccatgt ttctggcgag gtagcgattt ttttgattgc cagttttgcc
      301 tgcatgattt gacgttgcca ttttcctaga gttgtcacat tggaaactac ttcatcaatc
      361 aggacaaatt ggaaatcgcc aacttctcgc cctggtgtca gagagtatct ttgtggctgt
      421 tttggcaaac ggccttgttt catcaaatca tggatgactt ggcgaacata tacgttgatt
      481 tcctgttgga ccctgaaacc taagaacaga ttcacttgga caataaaatc agtgtccatc
      541 atatctactt gatattcttt cgtatatggc tcatccgtca cttccacatg aacaaaccaa
      601 tagactcgtg ctcttttagg ccgtttatct aaaatcgaat agatgaactg tcggccaact
      661 ttatcttcag ccatatcagg aaccaagaag accacatttg tctgatagat tggtaaagaa
      721 gtatcatcac gtaattctct aagctgcggg atataatctc tcaaagacac atcctcggtt
      781 gacgattcgc gaataatgtt cccttgttcc caaataaaca tcacgactaa gatgatcaaa
      841 ccaatcagaa cggctacaaa accgcctcgg aagaatttga caacacttga gataaagaaa
      901 acgacttcta tcgcaccgaa gaaaaaggta actaaatatg caagccaacg agaatgccca
      961 ttttggataa gataaaagta aagcagtgct gttgtcatca acatcgtcac agtgatcgat
     1021 agaccgtaag cagcttccat atgatgagac gttcggaatg tgacaaccac tagagaacag
     1081 gcgatccata aaatcagatt gactgctggg atatacattt gcccgatact gttcccagga
     1141 tacatgatct ttaaccgagg cagcagtttc aacttgattg cttctgaaac tagcgtaaac
     1201 gaacctgtaa tcaatgcttg cgaagcaatg actgcagcga ctgttgcaaa agcaacgccg
     1261 atcacgatcc aactttgagg catcatttgg aagaatggat tcaggttctt gatttgctgt
     1321 gcttcagggt tttgataaac attcaacaac cacgcagcct gcccaaaata attgataacc
     1381 aaacaaattt tgacgtaagg ccagctcaga cgaatatttc gtttgccgac atgtccaaga
     1441 tcggaataaa gcgcctctgc acctgtcgta gccaggaaaa tatttccaag aatgaacaag
     1501 cctaatttgt tatctgcact aaaaagcagc tggatagcat aataaggatt cagtgcacgg
     1561 ataactgtcc agtcttgact gaagttcatt aatcccatta cgcctaaaaa agtaaaccaa
     1621 gcgaacatga ttggtccgaa tgcttttccg acaatatctg taccaaagcg ttggaccatg
     1681 aataaaacaa aaataataac taaagtgata atgacgatca cgttctggtt attaccgaac
     1741 cggtcaaaaa actgcggaat tcctcgcaag ccttcaatcg cagtcgttac cgtcacagca
     1801 ggggtcaata ccccatctgc tagtaaggca gctcctccga tcattgcagg aatgatcaaa
     1861 tatttcccac cttttcggac aagtgtgtag agtgagaaaa ttccaccttc accatggtta
     1921 tctgcattca atgcaatcac gacatatttg attgttgtca gtagagtcaa tgtccaaaaa
     1981 atcaatgaga ctgatccgat aacaaaatca ggattgattc ctgacaatcc gccattatct
     2041 tcaacaatcg atttcataac gtaaagcgga cttgtcccga tgtctccgta gacgactccc
     2101 atcgctacaa gcaggccagc cattgatatc tttttggtat tgtgcgttgt tgccacgttt
     2161 ttccctcatt tcttgttata tggatatcct ttaggtaagc ttgaatgata gatgatattc
     2221 ttatccatga cacccgcttt cctaaattgc ttctaattat ccaagtaaca cactgaagtg
     2281 tcaacttatt tgaataaaat tattctttct ttggacgttc tctgatacgt cttgccacaa
     2341 atgaataaag agcaaggcaa aaaattgcac ccaataatgc tggtatcaaa taaaaaccgc
     2401 cgagttccgg tcccagtggt cctagtaaaa attcgccgag ccaactacct aaaaaaccga
     2461 taatgatatt tccagatacg cctcccggga cttcctttcc taaaattgcg ccagctatca
     2521 aacctaatat accgccgata aataacgacc ataaaaaatt cagcatctcc tttccctcct
     2581 ttatccatat ttctcacttt tattttacgc tttattcaga aggactccaa aaatatgcgc
     2641 caagaaactc cagaaaaaaa gaagttgtaa caaaagtgtt cagcttattt ccgtagaagc
     2701 tgcttctgga acaccgtgaa tcagaggttg tgaaatcagc ggtttgacct gagtattaga
     2761 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat atcgagggac
     2821 agctgattga acatcgttta ttggagaggt tgtaaaaaag ctgttctgca tcaagtaata
     2881 agaacagcca agtaaaaaca gctcctaatg tttttaactt ggctggactt attaccgcag
     2941 atgcaagctt ttgaacaccg tttattcgaa aaaaaaggac tgtgacacgg cttttgtcac
     3001 agtccccttt tttcgaataa ttattgtccc cctgtcacct tcacgatcac aagtacatta
     3061 ttcctatgaa ttaaattatt tttcttcatt tttcgttgaa ccatcttcgt tcaacattgc
     3121 ttttcttgac agatttacgc gtccttgttt atcgatttca gttactttca caaggatctc
     3181 atcacctagt ttaacaacat cttcgacttt attgacacga tcgtttgcta attgggaaat
     3241 atggacaagt ccatcttttc ctttgatcaa gttgacgaat gcaccgaatt tttcgatacg
     3301 gacaactttc cctagataaa cttcaccaac tttgacttct ttggtcaaat cttcgatgat
     3361 cttgatagct tttttgatca tttctgcatc agctgaagca atacttactt ttccatcttg
     3421 atcgatatcg attttcacac cagtttcatc gatgattccg ttgatcgtat cgccgccttt
     3481 accgataaca tctttgattt tagcaggttc gatctggatc atttcgattt ttggtgcata
     3541 ttggcttaat tcttcacgtg gagcagcaat cgttgaagtc aattcttcta agatttccat
     3601 acgtgcttgt tttgcttgag ttaatgcttc agttaggatt tgttctgtga ttccttgaat
     3661 cttgatatcc atctgtaaag cagtgatacc atcttttgta ccggcaactt taaagtccat
     3721 atcgccaaga tgatcttcca gcccttggat atcagttagg atcgtatagt tttcaccatc
     3781 agaaacaagt cccatcgcaa taccggcgac tggcgctttg attggtacac ctgcatccat
     3841 caatgctaat gtgccagcac agatacttgc ttgggaagaa gaaccatttg attccaatac
     3901 ttctgctact aaacggatcg tataaggaaa ttcttcttct gaagggatta cttgtgctaa
     3961 cgcacgttca cctaatgcgc catgtccgat ttcccgacgc cctggagagc ctgcacgtcc
     4021 tgtagaacca acagaaaatt gagggaaatt gtagtggtgg atgaatcgtt tgctgacttc
     4081 cacccctaaa ccatcaatga tttggtgctc tcctagaggt gcgagtgtac aagctgataa
     4141 agcttgtgtt tgtcctcgtg tgaacaaacc tgatccatgt acacgtggca ataagctgac
     4201 ctctgatgac aatggacgga tttcatccaa tttacgcccg tcaggacgga tcttatcgat
     4261 ggtgatcaat tcacgtacga catctttttc aagatcttct gtgatttgtt taacttcttt
     4321 caacaagtga tcttcatctt catgacctgc aaatttctca gcataagctt cgcggatatc
     4381 gatcttcacc tgctcgatat tatcttcgcg agcaagtttt tcttcagtca ttactgctgc
     4441 tttcattgta gcgtaagaag catcaaagat ttcttttttc aatacttcat caacttgcag
     4501 taaagtcact tccatttttt ctttccctac tgcttgaacg atttcttctt ggaaagcaac
     4561 taactctttg atagcgtcaa aaccaaacag caatgcacca agcatgtctt cttctgaaac
     4621 ttctttcgcg ccgctttcaa ccatattgat ggcttgtttc gttccggcaa cagtcaattc
     4681 gatatctgtt tgttctgctt gttctactgt tggattcaag acgtactcgc catttacacg
     4741 accaacttct acgcctgcga ttgggccgtc aaatgggata tctgagattg atagtgctaa
     4801 agaagaacct agcattgctg ccatagctgg cgagcagtct gtttctacac tcatcacgat
     4861 gttcgttact tggacttcat tacggaagcc ttctgcaaac atcgggcgaa ttggacggtc
     4921 gatcaaacgg gcagtcaatg ttgcttccgt acttggacgg ccttctcgtt tgatgaaacc
     4981 tccagggatt ttccctgctg catacatttt ttcttcataa ttgatcgtta atgggaaaaa
     5041 gtctgtatct tttgcttctt tagaggccac agctgcactt aagactactg tatctccata
     5101 acggactaaa acggctccgt ttgcttgttt tgctagctga ccgacttcga tttgtaaggg
     5161 acggccgccc catgtcgttt tgaatacttg tttttctgac ataacttctc cttttccttc
     5221 tcgtgaaagc aagacgctac taaacaaatg aaaactgccg aaagccgtct tttgagcagc
     5281 tttcatttgg ttagtaggag tccatctagc ttgatcacgg aattttactt aattgatata
     5341 cgagtaaaaa aagtgagatt catgtgaatc ccactctttt tgactaaaat taacgacgca
     5401 agcctaaacg tttgatcaat tcgcggtaac gttgaacgtc agttttacgt aagtaagcta
     5461 acaagttacg gcggtgacca acttttttca ttaggccgcg gtatgaatga tggtcttttt
     5521 tgtgcacgcg tgcatgttca ttcaagtggt tgatttcttc agttaataca gcgatttgta
     5581 cttctggaga accagtatcc ccttcatggc gagcgtattc gttgatgatt tcattcttgc
     5641 gttcttttga aattgccatt tctttcacct ctttcgatta ttcccctgcg actgagtaaa
     5701 acgttggtga ttcgcttaac caagtggcag gtcatgtgcc tagagcacac agctatactt
     5761 tacctgaaac cgggttgaaa atcaagtagt taatcattaa gaaaaggtag ttttcaaaca
     5821 atcttactac tacttttcca tttttgtggt aaactataat gcgaaaatag acttgaaaca
     5881 cagtctttta agaaaggggt acatattcca tgattacaat ggatgatatc attcgtgaag
     5941 gaaatccgac cttacgcgaa gttgcaaaag aagtttctct tccattaagc gaagaagata
     6001 tctctttagg aaaagagatg cttgagttct taaaaaacag ccaagatcca atcaaagctg
     6061 aagaactaca cttacgaggc ggtgttggct tagctgctcc tcaattagat atttctaaaa
     6121 gaatcattgc tgttcacgta ccaagtcctg acccagaggc agatggacct tctctaagta
     6181 ctgttatgta taatcctaaa attttgagcc attctgttca agatgcttgt ttaggggaag
     6241 gtgaaggctg tttatctgtt gatcgagaag taccaggata tgttgttcgc catgccaaaa
     6301 tcactgtgtc ttattatgat atgaatggtg aaaaacacaa gattcgtttg aagaattatg
     6361 aatcgatcgt cgttcaacat gaaatcgacc atatcaacgg tgtgatgttc tatgaccaca
     6421 tcaacgatca aaatccattt gcactaaaag aaggcgtatt ggtgatcgag tagagaattg
     6481 gcaggttcct tcgatactat gctacaagaa agctttataa aaaaggggag tgctcaaaag
     6541 ctgcacttcc cttttttatg tttctccact aatcgaaatt tagatagaca tctagaaatc
     6601 gcaattccaa aaaattctgt gtattggata cgcatctttt cttatttttt cttatttctt
     6661 ccatatgggt gtttatctca gagatgatag aatagtttct aggataaaaa gtagcagcta
     6721 cgatgttatc cttcgtagag gattcctgct tcagctagtt tttgtttgat gtttttcagc
     6781 atatgctttt ctgggtagcg gatcgtatgc agatggatgc cgcctgttaa ttcggataac
     6841 aaagtagcat tacttttttg ataacgtctc ataaaggaat cgatatcttg tggcgttttg
     6901 atgtgcaaag ttcctgctaa ctcaccgtaa aggggatgtt ccacaattac atcaatgatt
     6961 tcaccaccat tttcaacgat tagtctcagt tcttcttctg tttgatcttt tccatgctgg
     7021 acagcgattt tactgatgtg cccagtattg gtttcttctg tttcgaggat ataacctctt
     7081 gctgttgcca cgatactttt acctgcagct cgtagtaaag caatatcgcc tacaatgatc
     7141 tgacggctga ctccaaaagt tttcgcaaac ttgctagcac tcactggttt ttctgtattt
     7201 gccaaggttt gcatgatttg ttttcgccgt gtctctcctt cgatcacact tattcctcct
     7261 tcattttgtt ttggtattcg cgcgagtgtt tcatcaaatc aacatacgtg tctgttattg
     7321 tttccttata tcccttattt ttcagcagac taacaacttt atccaatact tgctgctctc
     7381 tttttgcatc ataaactgct tggttttctt cttttttcac ttgtcctatt ttagttaccg
     7441 tatccattct tttttctaat aaagcaacca actctcgatc gatcatatcg atcttatgac
     7501 gtaatgtttc aagagactct tctgttagtt cttcctctac tgtcttgatg atacgagcag
     7561 gatttcctgc aatcactaca ttgtcgggaa aagatttggt cacaaccgaa cctgcgccga
     7621 ctactacgtt gtcccctaga gtgacacccg gcgtgacgat aactcctccg cctaaccaaa
     7681 cattattacc gattttaatg ggttttgcgt actccagacc gctgttacgt ttcactggat
     7741 gtagtggatg cgtcgccgtg taaagctgga cattcggagc gaacatgcag ttatcaccga
     7801 tttcaatcgt gctgacatct aaaaatgtac aattgaaatt tgcataaaaa ttctccccaa
     7861 caaatatatt atatccgtaa tcaaaattga taactggctc catatagatt tttttccctg
     7921 tacggccaaa agtctctttc aacaattggg atcgcagttc cgctgattct gcttggttga
     7981 tgatttggct ttgacttctt gcaaacatac ggtcggcaac cagctcagga tcattggcaa
     8041 aataaagctc gcctgcaatc attttttctt tttcggtttt gaattcagtc ataatctgct
     8101 cctaattctt gtattttttt gaaaacaacc gctccgtctt cttgaataaa cgtataaggc
     8161 tccgctaaat aaacaggcgt ttgattgatt actaagattt tggctgacgg ctggcgaaac
     8221 tgtaccagat cacaaaacgg atgaacttga aaactcgttc caacaatgac gatcagctct
     8281 gcttggctga cggcataagc cgcctgctcc atgactccat cttgaaaccc ttcaccatat
     8341 aacacgatgt ctgggcgtat ttgtcctccg catgcttgat gtttgtcgct gtgaagatat
     8401 tctttccagt ctaccgtttg ttgacatttt cgacaatgac attgatacag actcccatga
     8461 aaattgacga gttgcgggct tttagccttt gcgtgcaaac cgtcgatgtt ttgggaaaca
     8521 acccaaacat ttttttcttt cgctagatgt gctatttcca aatgaatgat attgggtttt
     8581 gcttcaggat gataaatctt tttgacaaat tgatagaatt tatccggttc attcaccatt
     8641 gcttgatgac tcagcaagta ttccggctgc tccatcccgt ggtagattcc tgacaaagat
     8701 cgatagtctg gcactccaga aggtgtggat acccctgcac cggtcaaaaa tgtaatttgc
     8761 tgataagcta atatctcttt tgctgcttcg tctactgtta attgtttcat gttttgtcac
     8821 ctcactttcc attatagccc atactcgaac aaatttattg ttgaaaagtc agaaaaatgg
     8881 gaataaaaaa gtgttatggg catttgagca atttatcaca atgttcactg acaaaagaat
     8941 gaaaacgtgt tacaatactg ctgtataaaa gtgaggtgaa tcgatatgtt accattcttt
     9001 atttattggg gcgttattct ggcttgtatc gcctggttag cgttaagcgt ttatttctct
     9061 gttttttatc ttgtacgcaa agaaaatgga aatctttggg cttttgcttt attcaacgtg
     9121 attgccgcaa ttgttttagc cattacatta gccgtatatc gaacatgggg ctggggaatc
     9181 acccaatact caagtttgat ttatctaata ttagccattt atggcgtcgt tgttcttttg
     9241 caagcgatcc taggacgtga gccaaagaaa gcggcagctt aaatgatcga gatggaactg
     9301 gacacaggaa attttctgtt ctagttcttt ttttattgta ttacatacat ttttgtgtat
     9361 aattatacta tcgtttgcaa tggatagatg attcacattt tgattgtttt ttcggtaaaa
     9421 gtaaagattt ctatatttat cagtattttt tcaaaaacca gcagttatag attgacaaag
     9481 cctctcgagt atcgtaaact ttttttgtat aaataactga atacgagatg cctcaatcaa
     9541 tgaggtagag gtcgcgagaa tcattaaccc tgtggagtga agcaaacatg gagaagcagg
     9601 aatagggagg atcgccgaaa tgtgcacttt tttgcttgga gtgtatgttg ggacgcaagt
     9661 taagagcttg cggactgtct tagcagtgat gctaagttgc gctatgtttt gtgagaggtt
     9721 tatcacctta tggaatttta gcggcctttt tatatctata aatcggtcgc tttttatttt
     9781 ttcagataaa gctttaccgc atttcatcta gaccgctcgt gtatcatata atcagaaagg
     9841 gagaaaaaaa tgaaaaaaaa tatggtttca gttgccgtac tatttgcttt tgtcttctca
     9901 ctattcagtg tgggatctct ggcaaatgca gatgaaaaaa caccgagcga tgagtttcgc
     9961 gtaggtatgg aggctgggta tgcacccttc aactggtctc aacaaacaga tgagaataat
    10021 gccttgccaa ttcaaggcca aaatagttac gccggcggtt atgatgttca aatcgctaaa
    10081 aaagtagcag atggtttagg gaaaaaatta gtcatcgtcc aaacgaaatg ggatggtctg
    10141 gctcctgccc ttcaatcagg caagatcgac gcgatcatcg ccggaatgag ccctactgct
    10201 gaacgacgaa aagaaattga ttttaccgat ccttattacg aatcacaatt agtagtagta
    10261 gttcaaaaag atagcaaata tgcaaaagca aaaagtttag cagatctttc tggagcaaag
    10321 atcactgctc agttaaatac attccactat actgtgatcg atcagatacc agatgtgcag
    10381 aaacaacaag caatggataa tttctcagca atgagaacag ctttagcttc tggaatgatc
    10441 gatggttatg tcagcgaacg tccggaaggt gtaacggcaa ctagtgtgaa caaagacttg
    10501 gaaatgcttg aattcagcgg tgcagatggc ttccagacaa acccggaaga tgttcagatt
    10561 tctgttggta tgcgtaaagg cgacccggat atccaaaaag taaaccagat tctttctggc
    10621 atctctaacg atgaacgaac caaaattatg gaccaagcag tcaaagatca gcctgctgca
    10681 actgatagtg aagaaaaacc tggcatcatt tctgatttca ctacgatttg gaaacaatac
    10741 ggcaatatgt tcttacgagg tgctggactt accttgttca tcgcattgat cgggacagtt
    10801 gtcggtacta cacttggatt actgattggt gtcttccgtt cggtacctga atcaggaaat
    10861 gcactgactc gcttcttcca aaaaatcgga aatgcacttc tttctatcta tgtcgaagta
    10921 ttccgcggaa caccgatgat ggtacaagca atggtcatct tctacggtct ggcactcgct
    10981 tttggcattt cattgaaccg aacgattgcc gctttgttta ttgtctctct caatactgga
    11041 gcttacatga cagagatcgt tcgtgggggg atttttgcag tagatgaagg gcagttcgaa
    11101 gctgcgcaag ccattggtat gacccatggc caaacgatga gaaaagttgt tattccacaa
    11161 gttttacgga atatcttgcc agcaacagga aatgagtttg ttatcaatat caaagataca
    11221 gcagttttaa gtgtaatcgg cgtagctgat ttgttcttcc aaggaaactc tgcttccggt
    11281 gcgaatttcc aattcttcca aacatttacg atcgtcggga tcatctactt agtcatgacc
    11341 tttacgatca ccagaatcct gcgggcggta gaaaagaaaa tcgatggtcc ttcagcttat
    11401 accaagcttg aagaaatcga caattcaaat ttgcaggaat aaggaggaag aatcatgaat
    11461 gaaaaaatta ttgaaatcaa acatctaaga aaaagcttcg gtgaaaatga agtattgaaa
    11521 gatatcagcg taacagtcaa taaaggtgaa gttgtgacga tcatcggttc ttccggttct
    11581 ggtaaatcta ctttcttgcg ctgtatcaat ttactggaaa aaccaacagg cggcgagatc
    11641 atttataatg gagaaaatgt attagctcct aaatataatt tacctaaata tcgaacgaat
    11701 ctaggtatgg ttttccaatc cttcaacctg ttcaacaata tgaatgtctt ggaaaactgt
    11761 atgtctggtc aaacaactgt cctgaaacga gataaagaaa ctgccaaaaa agtagcaatc
    11821 gaaaatctgg aaaaagttgg tatggatcgt tatatcgaag ccaaaccagc acaattatct
    11881 ggtggacaga aacagcgtgt cgcaattgct cgggcattat caatgaatcc agatgtattg
    11941 ctattcgacg aacctacttc ggcgttagac cctgaaatgg ttggcgaagt gcttaatacg
    12001 atcaaagact tagcacatac tggcttgaca atgcttatcg tgacgcatga gatggaattt
    12061 gcaaaagatg tatctgaccg tgtcattttt atggataaag gtgtgattgc agaagaaggt
    12121 acaccagaag atatctttgt ccatccgaaa gaagaacgta caaaagaatt cctttcacgt
    12181 attttaaatg cttaggatca atagctgatt gggacaaaga gctgcggtgt ttaccgcagt
    12241 tcttttttat gaattactca taaaaggtaa atttctgaa
//
LOCUS       DAZKSR010000092        12163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000092 DAZKSR010000000
VERSION     DAZKSR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.093"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..730
                     /locus_tag="ACG8XG_002326"
     CDS             158..730
                     /locus_tag="ACG8XG_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0774216.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="ACG8XG_002327"
     CDS             875..1579
                     /locus_tag="ACG8XG_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0774217.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="ACG8XG_002328"
     CDS             1807..2145
                     /locus_tag="ACG8XG_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774218.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8XG_002329"
     CDS             2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8XG_002329"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0774219.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="ACG8XG_002330"
     CDS             3131..3364
                     /locus_tag="ACG8XG_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0774220.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="ACG8XG_002331"
     CDS             3562..5466
                     /locus_tag="ACG8XG_002331"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0774221.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="ACG8XG_002332"
     CDS             5776..6138
                     /locus_tag="ACG8XG_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0774222.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="ACG8XG_002333"
     CDS             6155..6766
                     /locus_tag="ACG8XG_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFQ0774223.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="ACG8XG_002334"
     CDS             complement(6868..7776)
                     /locus_tag="ACG8XG_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0774224.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="ACG8XG_002335"
     CDS             complement(7818..8132)
                     /locus_tag="ACG8XG_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0774225.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="ACG8XG_002336"
     CDS             8431..10362
                     /locus_tag="ACG8XG_002336"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0774226.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="ACG8XG_002337"
     CDS             10655..11206
                     /locus_tag="ACG8XG_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774227.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="ACG8XG_002338"
     CDS             11519..12061
                     /locus_tag="ACG8XG_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFQ0774228.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3952 a         2034 c         2555 g         3622 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt att
//
LOCUS       DAZKSR010000093        11780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000093 DAZKSR010000000
VERSION     DAZKSR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.094"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..534)
                     /locus_tag="ACG8XG_002339"
     CDS             complement(157..534)
                     /locus_tag="ACG8XG_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774229.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(834..1715)
                     /gene="galU"
                     /locus_tag="ACG8XG_002340"
     CDS             complement(834..1715)
                     /gene="galU"
                     /locus_tag="ACG8XG_002340"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0774230.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1758..2777)
                     /locus_tag="ACG8XG_002341"
     CDS             complement(1758..2777)
                     /locus_tag="ACG8XG_002341"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0774231.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3410..4414)
                     /locus_tag="ACG8XG_002342"
     CDS             complement(3410..4414)
                     /locus_tag="ACG8XG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0774232.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4427..5476)
                     /locus_tag="ACG8XG_002343"
     CDS             complement(4427..5476)
                     /locus_tag="ACG8XG_002343"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0774233.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5493..6314)
                     /locus_tag="ACG8XG_002344"
     CDS             complement(5493..6314)
                     /locus_tag="ACG8XG_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0774234.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6301..7080)
                     /locus_tag="ACG8XG_002345"
     CDS             complement(6301..7080)
                     /locus_tag="ACG8XG_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0774235.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7097..7567)
                     /locus_tag="ACG8XG_002346"
     CDS             complement(7097..7567)
                     /locus_tag="ACG8XG_002346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0774236.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7579..7998)
                     /locus_tag="ACG8XG_002347"
     CDS             complement(7579..7998)
                     /locus_tag="ACG8XG_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0774237.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8079..10856)
                     /locus_tag="ACG8XG_002348"
     CDS             complement(8079..10856)
                     /locus_tag="ACG8XG_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0774238.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11047..11271
                     /locus_tag="ACG8XG_002349"
     CDS             11047..11271
                     /locus_tag="ACG8XG_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774239.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3416 a         2480 c         1910 g         3974 t
ORIGIN      
        1 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat
       61 ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc
      121 attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct
      181 actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat
      241 ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa
      301 agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc
      361 aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa
      421 catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat
      481 cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa
      541 cacacacctt tttttataat taaaaaattc gcctttttat tctactatga atcttctggc
      601 ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac
      661 cattagcata atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca
      721 cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc
      781 tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt
      841 tctgttctta gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc
      901 gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg
      961 tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt
     1021 gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat
     1081 attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt
     1141 tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga
     1201 tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa
     1261 gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc
     1321 aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc
     1381 acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc
     1441 gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat
     1501 tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag
     1561 atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc
     1621 acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc
     1681 ccagcggcag gaatgactgc ttttttgacc ttcacttgct gttcctccta aacgacgatt
     1741 aactattgat catctattta acgaagagaa aattcattct ccatttttcc gtcacgtaac
     1801 atgatttctt ttgctgcttt tttgatatct tctccattat acaaaacatt atagattgtt
     1861 tgagtaattg gcatctcaac acctagctgt tgagctagct ctactgctgc ttttgtcgtt
     1921 gccacaccct caacgaccat tcccatgttt tctagaactt cttccagctt atgtccctgt
     1981 cctaacaagt taccggcacg ccaattgcga gaatgaacac ttgtacaagt aacgatcaag
     2041 tcccccacac cactcaaacc gataaatgtc agaggattgg cacccatcgc gacaccaaga
     2101 cggctgattt ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc tccgaatcct
     2161 aatccatgga tagcgccggc accaattgca atgatatttt tcagtgctgc accagtttct
     2221 acgccaatca catcattatt tgtataaatc ctgaaataat cattcatgaa cagtttttgt
     2281 acataggctg ctgctttttg atcaatactt gcagctgtaa ttgttgtgat atcatgtact
     2341 gccacttcct cagcatggct aggaccagat aatacaacaa ttgcctgtct tttttcttct
     2401 ggaatttctt cagccaaaac ttctgagatt cgtttatgcg tatcttgttc tagtccttta
     2461 cttgcatgta taatgattgg cttagtattc attttaccaa taagttcctg tgcgactgaa
     2521 cgaatcgctt ttgttggaac aacaaataaa atagcatctg cgttatccac tgcttctgcc
     2581 aattctttat atccgataat cgattctggg atcgtcaaat caggtaaaaa atgctgattt
     2641 gtatgatact tattgatttc atcaatctgt tcaggtacat ttccccaaat acggacatca
     2701 tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg accaagaact
     2761 gcgatttttt gtttcatgga tattcctcca aataaataat tgataatttc cattatagta
     2821 tacatttctt aaagttggga ctaatttcca ttgtttttaa gtgtttgttt acttctttta
     2881 tcgtttttac ttgaaataat caatgatatg ttacaatagt ctacatttta tttttacact
     2941 cgttatgctt tgcttgttcc taagaggaag cagttttgac aatgttacta ccagcattac
     3001 tattaggagg ttaggaaact tctcctagtg agtattgatt taaaatacat aagagacaga
     3061 ttgaacattt ctaaatctgt caggtcctgc aacggacaga ttgtctctgt tgacggttga
     3121 cataggaact tttgttttag tcaacattct ggatacaaaa cgctatgtaa actggcgcaa
     3181 ctcgttgtac ttggactttt actacggatc catgcaacaa ctaaatagta ctattgcata
     3241 acaaaaaatg gttggcaccc acatgtccca ttaaacagat tataagttga actgtagata
     3301 atgcaaaaaa tgtgttttat aacgtaattg atgccaatat tttaaaaaaa taaagggagc
     3361 agttatcgct gctctcttta ttttttatat tctttttatc cagtctatcc taataatcaa
     3421 actgacgata gtagcggcgt atctctaatg gatggcagtt caactgctcc acatgagcat
     3481 cgatgcgttg tgtcactaca tgcatcaata agaaagacaa aacacgtccg cgatattttt
     3541 cactgatacc ttctactgga tagtcttttg tgtctatcaa tgtataattg ctgcaaattt
     3601 ttggcaaaag tttagccaca cgttcagata acactctctg ttcatcttcg cctaaaaata
     3661 atgtgactgg ggttgtttct tctacgatct ctaacgtccc gtgaagaaac tcggcagcat
     3721 ggatagattt cgatggaagc cagctttgtt cttcccagta acacatggca tatgaataaa
     3781 cagctcccca ttgattccct gctccaataa aataatgcat actatcacgt cgatgttttt
     3841 ctgcaaatga taagccaaat gcatctgctt ttttctctgc ttccactaac cctgttggta
     3901 aataacgttc gagctgttca tagtattctg aataagcttc gaattctcca ttcaaataca
     3961 tcaatcgatc tgctgccata aaaaatttga tttgttctgt tccaggagct ggataagtga
     4021 ccacaaaatc gcatagctga gacaacttac tgttcgcttt atcgataaat ccaatcagtg
     4081 tcgctcctac tttcttgatt tgttctactg ctttcactac ttcttgagtt gtaccggtaa
     4141 cagaggacaa aataacgata gaatcttttg ttaatctttt gtttccagta gtgtaatatt
     4201 ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc attcatataa gtgacagctt
     4261 gcatcgagga agcatacgtt ccaccgattc ctagataata gacagcgtca aatttttccg
     4321 cataaagttt atcgatgatt tcttctactt gcggacgtaa agctagtgcg ccttttattg
     4381 ctttgatttg ttcttcttca ttgaatttca gcatgatttc cacctcttat aatttacttt
     4441 tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct ttagctactg
     4501 cggttttata agcgaatagt tgaaacggaa cgacaagcgc taatacagac agatattcat
     4561 cacattctat tgctaatcca agtgttttct cgtttcttgc tttttttgtg gtaatggtga
     4621 gagtctctcc aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt tccttattta
     4681 cactttcccc ttcaataaag aatagcaaat gcgaagcatc tgcctccagg tatgggccgt
     4741 gcatataagc ttctaactca aatccttgtg acggaacgcg gattgtttca gtcaatttcg
     4801 tctctgcttc tttagctgtt ccccagtttg gaccatatcc aatggtgacg aaacgctgtg
     4861 ctaaacgaaa taaactttga tatccatcaa aaaattcttc cgttttttga ataatcgctg
     4921 gtaaatggtt gattattttg cgcaactgtt gcatgtagtc ttgttctatt tttttgctga
     4981 ttttattttt acttattcca attcctatcc ctaagagtag taactgtaaa acagtcgcag
     5041 aatagccttt tgtaacgaag cctactgatt ctaccccggc acctaaatca ataatttgtg
     5101 cagcttctaa agcaagtgga ctttgcacgt catttgtgat agcaacaaca ggtaaccctt
     5161 gtttttgtat catttttact gcttcaacag tagaagcact tttcccgctt tgtgacacag
     5221 caatcacagt atcgattgaa ggagaaagtt ttccataatt gaaaaaataa aaaggttctt
     5281 gaatatcgac agtaagatca ccataagatt ccaatgctgg cttcgctgct aaacaagcgt
     5341 tataagatga acccgttgct aagatcagta aattcactgt ttttttcata tgttctcttg
     5401 tcgaaaaatc attctttttc aagatcgtac ttagtacttc cttttcttca tagatataat
     5461 cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa tccctatcca cgaaccaaaa
     5521 attccaaaga aagctagtcc gatcaagatc gttaatggtt tcacttcttt cttcaataga
     5581 taaaacacta tgccaaatgc tccaagacat aagatatttg gaacgatatc atcaaaaata
     5641 ctttgaacag tcacagcatt ttctcctgaa ccgattttta aaggaatatt gatggtgatc
     5701 atcgtggcag tcattgctcc aacgaccatc agtccaataa tagacgcgcc taatgtcaaa
     5761 ctttccatca taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa gcgatatccc
     5821 caacctgttg ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa aatgaacaag
     5881 atagggccta aaatatttcc ttgcatggct aaagaggtac ccacacctgt ggcaatcaat
     5941 cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa gaggtcccat caaagatgtt
     6001 ttgatactat cgatcgcagt cacatcaaat tcaggatcat ccttattttc ttcctccatt
     6061 gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa aaactctaga
     6121 tgccgtttca aagctgctgc catttcttct ttcgactgat ataacttttt caaaatcgga
     6181 atcattgcat aaacataagc catattcatc tgacgttcat aattccaaga aaactccatt
     6241 tgaaaagaac gccaaaatac gtgatttagc tcttttttcg ttattttgct gtttttgatc
     6301 tcagaattct tcatcttctt ctacctcccc tgcagttgtt tgaagtggta gttgacggac
     6361 attcataata ttgactacca caactgctaa aatcgcacca aaaatagcta ctccggtcac
     6421 aggaatctct aagtaagaag ctatagcaaa acctaagaaa aaatatggtg caacttgttt
     6481 gttgatcagc aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc ctgtagctac
     6541 tgacaacccc ttttgaacga agtcaggaat catatttaat agatttccga tcgtattgct
     6601 tcccaccata taagaaacca ttaccacaac accaagcatc aaggaaagac cgataccggc
     6661 aagcaagtgc atatgctcga ctcctttata atttccttct tctgcataat tgtctgcttt
     6721 atggttcata attggaatca aaagccctaa gtaaatattt ttcaatatca atgtcaacgt
     6781 agcaatcggc aaacctagta aaagcgcggt ttctgtaccg gctccagctg taatagcaaa
     6841 agccgtccct aaaatcccac cggtcacaac atcaggtgga atcgaagcac ctactgagaa
     6901 cgatccgata aatgctaact ctaaagttgc gcccataatg atcccagttt ttacatcacc
     6961 taacacgatt cctgtaaaca gccccgtcac aatcggacga gaaagcaacg atgtccctag
     7021 tgcatattct gattgtgcga caaatgcaac aattcctaat aagactgctt gtacaatcat
     7081 tgaggttctt cctttcttac aaaacttctc ttacatccac ttttttatct gctggtactt
     7141 gacggatttc tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc tcctcttctg
     7201 gtaaaatatt taccgctttt ccgatgctgc gagtaccagg ttttgccttg atccctccta
     7261 agttgatggt tttgatttct ggatacgctt ctgccagctt atatgcatcc tcgatcgatt
     7321 ccactacgat gaataactta tatttatctg tcaccccgct tttcaacgcg gcaatcgagt
     7381 catcaattga tttgatcact agtttgactc cttgtggctt ggctaatttg atcgttgtct
     7441 ttcgtatctc attcgtaggt acatcatcat tagcaatcaa aatacagtct gcgcctattg
     7501 tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg atggtcgact cttgtcaata
     7561 caatcatttt tcttcctcct aaaattcgtc ttcttctatt ttctgattga gccgctcgtt
     7621 acaaaactgg atcatttttt ttgaatcagt tagcacctgc tggatcaaaa cagttgtctc
     7681 tacttcagcg tccaaccgag atactagctc aatcaatagt ggcaaattca atccggcaac
     7741 gagatagaca tgatcccgat tgatatattg taagaattcg ttgttgacac ttccgccaaa
     7801 aagatccgtc agaacaatga tttctttatc tttgtacctc ataaaaagct gccttactgt
     7861 ttccgctaaa tcaaaattct catcagtata gcaatcgaat acagtaatag aatgattttt
     7921 cccatagatc agctctaatg aatctaatat ccctgaagca aattttccat gggaagctaa
     7981 gataatttct ctatccactt tttcatctcc tcttttcact tacacatact aaaaaagcaa
     8041 gttgcatgcc aaaattagtg gcaacaactt gcttatgttc aaaattcttc ttctatcgag
     8101 agaaactctg tatcttcact caaaatatca tagatatagg caatctcaga attggggatc
     8161 atgacactat aatgctcttc aatgacacta aacgcagatt taatagcaga taacccttct
     8221 ttttgacact gcttccattt ctctaatcct tgataggttt cgattggcat ttgtcggatc
     8281 aatcgttcaa tgagacagct cacatggaca tacaacgcta attttttggg attgctgatg
     8341 cttcgattca actgcacctc taattctctc ataaaatgat cgatttctcc catcaccttc
     8401 tctgtatcta gaatagtcac ggaatccagt actttttcca atgaaaaatt acgaatgatg
     8461 cgctgattga atatttcctt ctctgaagca ttcatagtag tgtccaacca ttcagctagt
     8521 acagacatct tttcccccga gatgaggtct tctaatgaaa gatagtcgat attggtgata
     8581 tcaggatctg tggtgccaat cattcctacg acttcgtata tggaaaataa tgggtcactg
     8641 tttttcttat cttttaattg ctgatattca tatgggatga ttttcaactg acaagccgta
     8701 ggtaagcttt tttctagcag ttcacttaag tggaccgccg taccgattcc agtgaagcaa
     8761 gtagtaatga gtgctttggg tttgttttcc tctggataga gcagtcgcca ttctggctgg
     8821 atctgtcgaa cagtctcttc tgctatctct tctaatgata catttttttg caggcattcc
     8881 cctactgtga ttgctaaagg tgtcgtcaca ttattcaaaa tcaaaagcgg tacggataac
     8941 tgttttttaa aaaactgatg gatttctttc agagatccca tgtctactaa tatgatcaat
     9001 ccatttgaaa catcgctgtt ttcgcaataa tcaaggattt ctgaagcaat ctgctgtggt
     9061 gtgacatcta gtggcatatc aaatgattca aatagatcct cttgtaataa acgatttact
     9121 acatttgcca tactgctagc agttgcataa ccgtgagcag caatcatcgc ttttggcagt
     9181 cgtttttctt ttgaccattt cgcttttttt agatagatcg tcagtaaaat ccgatccata
     9241 cttgtgtctt caatatctaa tttgatctta cttaattcca gaattcgttg gacatagtga
     9301 taactagatg gataagactg agaaacttgt tgttctaatt ctttgattag ttggataata
     9361 tcatgttctt ccggtaacca tttgatagtg cttcgctgat agagatagta actcaaggca
     9421 tagacactgt ttccatcgaa tttgatttga taggcagttt ccatttgtcg aaaggtctct
     9481 cggatagatt gtgtccaata aaccagcagc tcgtgttttt cttgtctact ggtctcaaac
     9541 agcaaaaaat caaataattg aatcacttct tgctttaatt ttccttcgca agaatatagc
     9601 agacagttat ttttcttgaa atcaattagg atttgctcaa aacttttaac gattcgctgc
     9661 tgttcgggct gttgcttctc cattaattgc tctaaggtct ggtcttgcgt aatatagatt
     9721 ccttgctgtg ctgtattttt agtaataggc tgcgcaagta gttctttttg caaatgatgg
     9781 atcgtgacat ggatttcagg ctgttcacgc tgttcagcat atgcttttgc tgctgaaact
     9841 ttgacgatat ttttcagctc tcctatgttt ccggataatt tctgacttgt taaaagtgac
     9901 aaagctggtc cactgactga caaagggctc cccatttttc tttgttcttc gagaaaaaaa
     9961 gagtaaatca gttctagccg ttcattacga ctacgctgat taagtgcagg tagttcgatt
    10021 cggataggta ttcttcgaat gaaagttgtt aagaaatgac tagtaatctc ttcagtcgta
    10081 gcaaaaaaga gacgtgtttt gatccggcgt gacaaattag gttctcccat acgataaatc
    10141 acaccttgat caagataggt gaacagtttt tcctgacctt ctggtgttaa gcgatgtacc
    10201 tcgtctaaaa acaagatccc accatctgct gcttcaaaag cccctttttt attttcttct
    10261 gcaccagtgt acgctccctt aacatgtcca aacaaattgc tagtcaaaag ttcaggatta
    10321 tctgcatatt gtgcacaatt aaaagtgatg aatggtgcag aatcttctag cagatcatga
    10381 agcagacaat actgatagac caaattaact aaataacttt tacctgtgcc actttcgcct
    10441 gtaatcaaaa gtggtaatcc gccatctgga tagtaaagag ctgttttgat ttgttcgata
    10501 ctttctttca aactggcgtc gtatccgatc ataaaagaga ataagtcttt tggttgcgcg
    10561 aaaaaaggct gttcctcttt gagatcatcg atagaaaaat aaaaattcgt gcgtaactta
    10621 aaaaattgtt tttcaaaagc ttctttgtgg aaataaatca ctggtcgaga attgattttg
    10681 actagtagtt gctcatcatt taactggttc aaataatgac tgactgtgtt tctttttacc
    10741 ccaaaatgat cagctaacct ttgtgctgta aagacctcgt tcatatgttc taaatcgaaa
    10801 aaggctgtct ggttctctaa gtaacataaa atttccttct tcaacaactt attcacccct
    10861 cctccatcat agaaaacgga ttcattttgt caataaaatc tttaataaag aattgaaaaa
    10921 tatatcttat tatagacagc agcctactta tcccttataa tttttattct aaaaaacgta
    10981 cagttccttc cagtttttat actggaattt caacatatga aagaataagg aggctatgac
    11041 tattcaatga aatcggataa atataaaaac aggaaacata cttcctggaa aacttttcga
    11101 gaaatcatcg gatcttatct cgctgtagtt gtatttttgc ctttgcttta tatggctatg
    11161 acagaacaaa ctcgttttat tggcgtagat acgcaatata tagccgaaac aggattttta
    11221 ttcatcttta tcttgatttt tgcgttctat aaatccttcc atacaaaata agattcacag
    11281 atttcaactt tcctgtctgt ttttagctat tacaaatcct tatatgtggt ataacaacaa
    11341 tttattggca taatgaggaa aaacgagtta tgatgctttc agagagtaaa gaggtactgt
    11401 tgtcaccatc ctttttattg attacgtaca ccactcttag ttttttccca ttgattacga
    11461 aaacaaggta gaaacattga tgttcctacc ttgttttctt tttttatttt cagatcaaaa
    11521 tctcaagtgg cttttgactt gatgtcggga ggttgtcttc gttccatcaa acctgataga
    11581 atctgcgcca aaccatagtt ttctccacta ttatttattc ctctaagata gtacccacaa
    11641 taaacgaata aatatttttc tcattttcat actccttctt tgaaaatcaa ttagatttct
    11701 ctcaacccac ttttttacaa aaattacgta gcaaaaaaga ttgcaagaac aaattaaaaa
    11761 agaagtaaat agagtttcat
//
LOCUS       DAZKSR010000094        11663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000094 DAZKSR010000000
VERSION     DAZKSR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.095"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8XG_002350"
     CDS             complement(132..509)
                     /locus_tag="ACG8XG_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774240.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8XG_002351"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8XG_002351"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0774241.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8XG_002352"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8XG_002352"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0774242.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8XG_002353"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8XG_002353"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0774243.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8XG_002354"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8XG_002354"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0774244.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8XG_002355"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8XG_002355"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0774245.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8XG_002356"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8XG_002356"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0774246.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8XG_002357"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8XG_002357"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0774247.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8XG_002358"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8XG_002358"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0774248.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8XG_002359"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8XG_002359"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0774249.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8XG_002360"
     CDS             10661..11575
                     /locus_tag="ACG8XG_002360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0774250.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3834 a         1972 c         2733 g         3124 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc ttt
//
LOCUS       DAZKSR010000095        11562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000095 DAZKSR010000000
VERSION     DAZKSR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.096"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..556)
                     /locus_tag="ACG8XG_002361"
                     /pseudo
     CDS             complement(270..556)
                     /locus_tag="ACG8XG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(733..1911)
                     /locus_tag="ACG8XG_002362"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="ACG8XG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="ACG8XG_002363"
     CDS             1941..2351
                     /locus_tag="ACG8XG_002363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774251.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="ACG8XG_002364"
     CDS             2432..3052
                     /locus_tag="ACG8XG_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774252.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="ACG8XG_002365"
     CDS             3753..4037
                     /locus_tag="ACG8XG_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774253.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="ACG8XG_002366"
     CDS             4040..4957
                     /locus_tag="ACG8XG_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0774254.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="ACG8XG_002367"
     CDS             4950..5732
                     /locus_tag="ACG8XG_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0774255.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="ACG8XG_002368"
     CDS             5733..6413
                     /locus_tag="ACG8XG_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0774256.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="ACG8XG_002369"
     CDS             6406..7302
                     /locus_tag="ACG8XG_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0774257.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7497..9146
                     /locus_tag="ACG8XG_002370"
     CDS             7497..9146
                     /locus_tag="ACG8XG_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0774258.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9740..10675
                     /locus_tag="ACG8XG_002371"
     CDS             9740..10675
                     /locus_tag="ACG8XG_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774259.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10668..11438
                     /locus_tag="ACG8XG_002372"
     CDS             10668..11438
                     /locus_tag="ACG8XG_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0774260.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3662 a         1920 c         2414 g         3566 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttttc acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt
     7621 atcaaccgag atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat
     7681 tccgttcatg ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag
     7741 tatttgaagg gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt
     7801 tacgtagctt tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg
     7861 aaaatcctca actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc
     7921 ataattttgc aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt
     7981 tatttatttt tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag
     8041 tactaggaat ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga
     8101 agaaacaaac actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag
     8161 ccgccaatca gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta
     8221 aaccattaag agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag
     8281 cagaattgat ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt
     8341 tcgttctcac taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg
     8401 gagaattgga agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc
     8461 gaccagagtt tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag
     8521 aagcggtagt taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat
     8581 caggaaaatc tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc
     8641 aaacagcgat tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa
     8701 tcgaagatga tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac
     8761 gtttgctgga ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa
     8821 aaggaacaaa tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg
     8881 agtatccatc tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga
     8941 attattgtga gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg
     9001 atttcaaatt aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt
     9061 tgaattatcc taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac
     9121 agcgtcagtg gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa
     9181 aagacgatta tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg
     9241 agtctgctgt ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc
     9301 tgtcttcctt ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc
     9361 catggcaaag ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca
     9421 ttttatttat ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc
     9481 ttaacatgaa taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag
     9541 tagttgctgt cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct
     9601 atcgaagatg atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt
     9661 gtggaagcca agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct
     9721 ataaggagat aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta
     9781 tggaagtggg gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta
     9841 tgcgctatta gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt
     9901 agtcaataaa acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt
     9961 tcgagcaaag caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac
    10021 ggtccagcaa atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat
    10081 gaaacgcagc gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc
    10141 acgtatgctt tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga
    10201 accacgtttg ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga
    10261 gatgtgggag tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca
    10321 ttatctagaa gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact
    10381 gatcgagaat acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt
    10441 tgatttagcg ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga
    10501 tgagcagaca atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca
    10561 gcttacaaga caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga
    10621 actgttcttg aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt
    10681 attttactgc attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg
    10741 tccaaacact ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa
    10801 tgatcggtgg gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg
    10861 gtttgattat gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct
    10921 ctcaaaaatt tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga
    10981 ttatttgggg ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa
    11041 cgatcatttc cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta
    11101 cattattgat gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc
    11161 agtcttatga tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag
    11221 gtggcgtttt ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca
    11281 atccgatcgt ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc
    11341 cagtagcaac atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt
    11401 ggtatttgat caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg
    11461 ttttgacctg agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt
    11521 tgagcgaata ccgagggtca gctgattgaa caccgtttag tg
//
LOCUS       DAZKSR010000096        11417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000096 DAZKSR010000000
VERSION     DAZKSR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.097"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..801)
                     /locus_tag="ACG8XG_002373"
     CDS             complement(145..801)
                     /locus_tag="ACG8XG_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0774261.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            994..1272
                     /locus_tag="ACG8XG_002374"
     CDS             994..1272
                     /locus_tag="ACG8XG_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774262.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1291..2499
                     /locus_tag="ACG8XG_002375"
     CDS             1291..2499
                     /locus_tag="ACG8XG_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0774263.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2647..3012)
                     /locus_tag="ACG8XG_002376"
     CDS             complement(2647..3012)
                     /locus_tag="ACG8XG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0774264.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3159..3932)
                     /locus_tag="ACG8XG_002377"
     CDS             complement(3159..3932)
                     /locus_tag="ACG8XG_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0774265.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4106..4966)
                     /locus_tag="ACG8XG_002378"
     CDS             complement(4106..4966)
                     /locus_tag="ACG8XG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0774266.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4966..6384)
                     /locus_tag="ACG8XG_002379"
     CDS             complement(4966..6384)
                     /locus_tag="ACG8XG_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0774267.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6441..6890)
                     /locus_tag="ACG8XG_002380"
     CDS             complement(6441..6890)
                     /locus_tag="ACG8XG_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0774268.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7335..7598)
                     /locus_tag="ACG8XG_002381"
     CDS             complement(7335..7598)
                     /locus_tag="ACG8XG_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0774269.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7697..8992)
                     /gene="rho"
                     /locus_tag="ACG8XG_002382"
     CDS             complement(7697..8992)
                     /gene="rho"
                     /locus_tag="ACG8XG_002382"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0774270.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8989..10260)
                     /locus_tag="ACG8XG_002383"
     CDS             complement(8989..10260)
                     /locus_tag="ACG8XG_002383"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0774271.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10426..11295)
                     /locus_tag="ACG8XG_002384"
     CDS             complement(10426..11295)
                     /locus_tag="ACG8XG_002384"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0774272.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         3206 a         2411 c         1977 g         3823 t
ORIGIN      
        1 caggctcaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
       61 aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca ggtcaaaacg
      121 ctgatttcac aacctctttg ttcatcaaaa gataatattg tcttctaaca agtccatatt
      181 caaaataacg atatgatggt ctttaacggt gataatacca tcttttttca gttgttttaa
      241 aattctgttt acactgctag cagttgagat gccacaaaat ttggcgattt cttcattcgt
      301 gatcgaaaaa tcgatcagca tcccttcttc tgtccgcaca ccgaataaat catataattc
      361 gtaaagttgc gtacaaacag ctcccagctt tccgtttaat agcatttgct gcattttttt
      421 catcgaataa agcagacgat agcggtaata ctcttttaca tagtgttgta gctttgggtc
      481 ttggttgata ttttgccaaa agcgcacacg attgattcga taaaactctg catattctga
      541 ttcaactcga atattatacg gcgaatcgat gaattgtgaa tattcgtctt ttaaaagaga
      601 gacgatttca agatctttga tatagcgaag gttaaattct ctcccatcat ttgaaataac
      661 acttgctttt accgttccct ctttcaagat atacgtatgg gaatcttcca tcccttcgaa
      721 tgtcaaatat ttatgatatc ctttgcgtat aatcggaaaa tcatttttat ccaaatattc
      781 ctttaagact ctactatcca atgatgctcc cccatccaaa ctttcatcta aaaagatcat
      841 acagtacttt aaaacttttt aaagtccaaa ggtattttac taatgatatt aacatttgtt
      901 aagtaccttt ttccatcact tatgcgattt ttttcctttt ttttattcgt tatactgcag
      961 aagtaaaatt ttttaagaat agagggaaaa aacatgaatt actggatgaa cacaatcatt
     1021 aatcgattag aaacagcata tcaaacaaga tttgatatga aagcttcttt agtcttttta
     1081 aatgatgcgt atcaaaactc gatcgaattg atcaaagcag tggatgagaa tccaacaaac
     1141 gaatgcgaag aatttttaaa tctgtttatg tctacaagag atttatttat tcgccaactt
     1201 gttgatcgct atccatccaa ttatcacgat gtagaagttc aaatacaaaa attaaaagca
     1261 tattcagctt aaggggaatg tataacatac atggaaaaga aattatttaa taaatatttt
     1321 attggaatca cattgatcaa ttttattgtc tacctgattt attatctgtt gatggtcatc
     1381 atggcagtca ttgcgcaaga caccttacac gccagcttga gccaagcagg ttttgcttct
     1441 gggatctata tcatcggaac attgctggca cgcttattca tggggaaaca gttagaacta
     1501 tttggtcgga agtttacctt aagaggcggc gcgatcttct atctcctttc aacattagca
     1561 tatctttata cgccaactat cggggcattg tatttagttc gattcttgaa tggattcggc
     1621 tacggtacag tttctacagc tacaaatgct atcgttactg catatatccc tgcatctaaa
     1681 aaaggggaag gcatcaatta ttatggatta agtactagtt tagctgccgc tgttgggcca
     1741 tttttaggaa tgattttgct gaatatgact gattttcgtt tcattatctg gttttcaatt
     1801 gtactggtct tctttgttac gattgcctgc cttgtctttc ctgtaaaaaa tattccatta
     1861 tctgaagaac aaaaaatatc tcttcgctca tggaatatca atagttttat tgaaaagaaa
     1921 gcacttttca ttacaggaat tgcctttttg atgggtcttt cttattcaag tgttttatca
     1981 tttttatctt cttatacaaa agtaatccac ttagttgctg ctgggtcatt tttcttcgtc
     2041 gtatatgctt tggtcatcac atttacacgt ccattgactg gaaggatttt cgatgtcaaa
     2101 ggagaacaat acgtgatgta tcctagctat ttattcttaa cagcaggatt atttttactg
     2161 agtgtaacga cgaatagtgt gacattgctt atctctggag cattagtcgg cctaggttat
     2221 ggtacgttta tgtcaaatgg acaggctgtt tgtttaaaaa tcgtccgcga acatcgaatc
     2281 agtattgctt tatctaccta ctttattggt ttagacctag gcttaggcgt tggtccttat
     2341 ttgatgggga tgctaaaatc tcttacttca tttagaggat tgtatgtgat tgccggagtc
     2401 attccattga tatgtacagc actctatctt gttaatggcc gaaaacatag tcatgaagca
     2461 gagggcgaat tgattgaatc attgaaaaat gaaatttgat cttgatgaat agataaaaaa
     2521 aaccgttcag atctgaaaat aatctgagcg gttttttgag taaaaatagt ttatgattat
     2581 tcttaattaa acatctattt atttttaaag ctttcattta tcgataagaa tagtttaact
     2641 gttgatttat tccattttcc gttcatctgg tacgatgcct tgacttagca accacttgtg
     2701 gaagaaccat tctgccacac cgatcaatag agcaggaatc aatactttca tccaaatatc
     2761 gactgtatca ttcataaaat atagatagag ccacaagata actgcagctg aaccagcatc
     2821 tgcgatcgtc gctgcgatgt tccctacttt acgtaagatc aacagatccc ctagcacata
     2881 gatcatgact acaataatta ccccaacaat cgttgaatcc ataaagctaa caccgtataa
     2941 acctgtcagc acgacccaca gaatcgccca gatcatgatt gccttcacta ccaacgcctt
     3001 cacatgtttc attttctgca ctcctttctc ctggataaaa aagtaagatt atctaacaag
     3061 ataacctttt cattattata ccatcggctg agagaagcga gcaaagataa ggtcctatct
     3121 aaaaatgagg aagccactta gaacattcac gtaataattt atccgtacag acattcgata
     3181 tggtgttcct ttactttcgc atactgttct tttgtcagtt ctttacttgt caccagataa
     3241 tcgatttcat gaaaagcaat ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta
     3301 actaaaacgg caagccggtc tgattgtttt ctaaaagctt ttttgaccat tgcctctcct
     3361 tcatgagttt ctgttactcc ttggctgaaa tctagcccgc gacaactcat tatcgctaaa
     3421 tccgcataat atgacaatgc atcgttatag gcttttgaac cgttgattgt tgctcgtttc
     3481 ggaacaattg cccctcccaa tacagatact tttgccgttg tatattcact caacatagca
     3541 gctgtgacga tgccattcgt aatgatctgc aaaccatcaa atcttgctag gtttctgact
     3601 aagtgtaaag aggttgtact ggaatccaag aataagacca tatcatctcg aatcaaagaa
     3661 gaagctgtat ctgcaatctt acgtttttct gtttcttctt ctaatttggt gatcttcatc
     3721 aggtaaggca cgtcaatttt tcgattgtca ataagcatgg cgccaccgcg aactctacgg
     3781 atcaatccac ttttttccag aaaaacaagg tcacgtttaa tcgtggcttc gctataattt
     3841 actaactcga tcaaatcggt gactcttgca aattggttgt tttttagata attcacaatc
     3901 tcctgctgtc tattctccac ttcattcaac ataaaatcac cccatttact ttatatagta
     3961 acaggaaaaa agaggttgtg acagaagtga ttggcttcga gaaataagaa gaaatttttc
     4021 gaaaaaacta cttctgaaca tcgtgtgtgg ttaagagggt atgacaagac tattgtcata
     4081 ccctcttgac cacacatttt tatttttagt ataaactagc cttgcctact gtgttcaaaa
     4141 tttccatttt atgtttgacc acttcttgta cctttttatt agctgctgga taaattgcat
     4201 tcggctcgta catcgatgga ttttcactca atatttctct caaagcattg aaaaagacgc
     4261 ttttaagatc tgagctcaaa ttcactttgg caactccgta ttttactgct tcgctgactt
     4321 ctgcgtccgg attacctgaa ccgccatgta aaacaaatgg aagatcgatt tgttggttca
     4381 attctttcag taagtcgata tttaattttg gagttctgtt tttcggatac aagccatgcg
     4441 ctgttccaat tgctacagca agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt
     4501 gatcaggatc tgtataaatg attgtttcag cacctccttc agcagaacca ttattcccaa
     4561 tcgttccaag ctcagcctcc actgagacat ttaccgcatg agctaacgca accacttttt
     4621 tcgtaatctg tacattgtcg tcgaatgatt tcaaagacgc atcgatcatc acggaagaat
     4681 agccgtttcg aatcgcccgt aagacatctt ctatcgtacc gccatgatcc aagtggataa
     4741 cgatcggcag tttacttgta taagcatagg ctcttactgc cgcaacaaac tcatcactta
     4801 aataggcgat ttcatcagga tgaatttcta agattactgg tgcttctaat ctctctgctt
     4861 cttcgatgat tgctttcatc atatcatagc tgcaaatatt gaaggctggt actgcaaatt
     4921 gattctcttt agccactttc aataactctt tcattgtata taacattacg cttccacctc
     4981 atttgttaga ttttcatttt tcgtccactt gtcttgtttt ttctctttca ctgttttttt
     5041 caatagtcca agcataaagg ctgtagtgac tgtacctgat gcaattgcca agacatacat
     5101 aggtaaattg ttcataacat ttggaattag catcacccaa atccctccat gaggtgccat
     5161 tgaagtaact cctactgcca tggaaatccc tgcagcgact gctgaacctg tcatcaaact
     5221 aggtatcacg cggatcgggt cagataccgc aaacggtata gcaccttctg taatatacga
     5281 agcgcctaaa atccagcatg attgacctga agtccgttct tctttggtaa atttatgttt
     5341 gaataataaa gtggctaaag caatacctag gggaggtgtc attcctgcag ccatacaagc
     5401 agcaatcggt gcatttactc cagctgacat caaaccgatc gagaaagtcg aagtcgattt
     5461 attgatcggc ccacccatat cagctgccat catgatcccg ataagtaatc cgaacaatag
     5521 cccggatgta tttcccaagc tattcagcca atttgtcaaa gcatccaaca cccatgtgat
     5581 cggttgttca atcacaaata ccatcaaaag cccaataatt aagatcgata aaaaggggac
     5641 gacaatcagc tgataaatag cagaaaattc tttttttacg ctaattttat ttgccagaaa
     5701 atcacagata taccctgcta gcacgccacc aatcattccg ccaagaaaac ctgaaccacc
     5761 tgtagaggcc agtaatccgg caacaattcc tggggcaaaa cctgcttttc cagcaatcga
     5821 tgtagcaatc cccgcgccta acgcaggaac catcatggca aacgctgtat ctcctccgat
     5881 ccttgaaaat gcagctgcta acacattata gtctgctgag ttgggatcag ccgctgtaat
     5941 cccaaaggca aaactcagtg cgatgatgat tccgcctgcg atcacaaatg gaagcatgta
     6001 attcacacca ttcatgaaat gattatagat tgtcggcttt ttctcatttg cagctggttc
     6061 tttttcatta gacacaacga atacgccaat atctctcaat gcttcgtcta ttactacttg
     6121 cggttctttg attgctcggc ttgtagagac acgctttact tttttcccgg caaatcggtc
     6181 caattcgatt tctttatcta ctgcaagaat gattgcatct gcctctttga tttcttgttc
     6241 agataactga ttttctgttt ttacaccttg tgtttcgaat tttgcttgat ggtttagatt
     6301 tcttgctgtt acttctaatt tttctgccga catataagta tgagcaattc cagtcgggca
     6361 agcagttaca ccaacgattt tcatgattat tttcctcctt tttcttcact atcgatactc
     6421 ggacaaagac tctactattt tcattcttga ctaatctgtc tgatgatatc tgctggcgta
     6481 gtggcttctt ttattccctt tctaaaatct gcattcatca atcgtctgga gagtgtgctg
     6541 agaatctcta gatgcatatc tgctgatttt tcaggaacta cgatggcaaa aatatattca
     6601 attggcattt catctatccc gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt
     6661 gctggttttg tcacatattt gctttttgca tggggaatcc caaatccatc ttctattcct
     6721 gtggagatct cctcctctcg tttgaatagt gccttaacga ctttttgaac atttcttacc
     6781 ttatttgttt ttttcaacac atcccctatt cgctcaaata atgatccttt tgaatctatc
     6841 gatggatcga aaacaatcag ttcttctgcg atcaattgat caaacctcat aaaaaacctc
     6901 tcccccacaa aaaatgttaa cgttttcttg gaactgaggt cagtatagca tcgctagctg
     6961 taccgttcaa tattatgaat gaacagagga aaaagaccca tcatccgcgt gtataagtca
     7021 ttaaaatgaa cgatctagct cacaaacaat cgcaagtcta taacaaaaca aaaagccttc
     7081 cttgaaagga aagcttagct tctaatttta ggtaaatggt tttttacttc gaaataaccc
     7141 ctcttcattt cgttaatata aggtgttttt atcaagtgga taattaaaac tatatactag
     7201 tttttcattg ttttgtgcat tatgatccta cgactttttc agcatcaaac gaagttttaa
     7261 attggcaaaa aacataaaaa aacagactat ccctatcaga tagtctgcta aacgttgagt
     7321 tatcagcgtt tttattattc cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa
     7381 cggtccacac gtccgtctgc ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag
     7441 tcagaagtta cttcgacacg gatgactggg tatgtattac cgtcttccca ttcaactgtt
     7501 tcttgtgaag tttttgttga acctgacaag aatttgaaac cagttgttga atccatgaat
     7561 actactgggt ggtaatctgg atggatgttt tctttcatga ttttttcgct cctttgccct
     7621 gctccatttg ctgaaccaga gttgatttgt cgatttacaa cattaacatc ttaacatgtt
     7681 tcagtcaagg ttgcaattat cttttcagat ttctggtcgg atttttttta ccaaaggaga
     7741 catcttgaaa ttctttgaaa aattgctgat tgttttttgt tttacgcaaa aagcggatca
     7801 gttgttccgt atattctaat gaatctccag tcatattttt tcgaagtttc caaatttctt
     7861 ctaattcttc acttggcatc aataattctt cttttcgggt acctgatttt ttgatatcga
     7921 tggctgggaa gatccgacgt tcagataatt cacgtgaaag ttgtaattct agatttccag
     7981 ttcctttgaa ttcttcatag atcacgtcat ccatacggct tcctgtatcg actaaagcgg
     8041 tcgctaaaat cgtcaaactg ccaccttctt cgatgtttct tgctgccccg aaaaatttct
     8101 taggtttata caatgcggct gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa
     8161 cgactaagtt ataggcccga gcaagtctag taatactatc catcaagata acgacatctc
     8221 gtttatcttc gactaaacgc attgcgcgtt ccaatactaa ttcagaaact cgagtatggt
     8281 tttgcggctg cagatcaaat gtagaagata ccacgtcgcc tttcacgctt cgttctagat
     8341 ccgttacttc ttccggtcgt tcatcaatca gtagaacgat taattctact tccggatagt
     8401 tttcggtgat accatttgcg atttctttta atacagaagt tttccctgct tttggcggtg
     8461 cgacgatcaa tccacgctga ccaaaaccga ttggagaaaa gatatcgatc attcgggtag
     8521 atagtctttg cggtgttgtt tccagttgaa gctgtctttg cggataaaga ggtgttaagg
     8581 ctggaaaatg cggacgttct tttgcttctt ccggatcttt tccattaacg ctttccacgt
     8641 gcatcaaacc ataataacgt tccgattctt taggtggacg ggcttttcct gccactttgt
     8701 ccccatttct caaactgaat cgtctgattt gtgaggcaga aatatagata tcttctgcac
     8761 ttggtccata attgattggc cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata
     8821 ttccttccat ataataaaat ccttgtttct ctgcttgtgc acgaatcaca gctagagata
     8881 attccttttt attcatctta ctgtaataag gaattttaaa ttctttggca taactataaa
     8941 cttcttttaa cgttttgttc tctagttctg ccattgtcaa ataatcgctc attcagctcc
     9001 cacttcctct atcatttcaa ttgatgcccc taaggctgtt agtttctcga taatatgatc
     9061 ataaccgcgt aagatataat ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc
     9121 agcaatcact aaacaagcac ctgcccgaag atcagaagcc acgacttctg tgccgtgaag
     9181 tttatttgga ccattcacga tgatcatatt tccttccacg gtaatatcag cgcccattcg
     9241 tgctagttcc ggcacatggt tcacacgttt cgcatagatc gtatcgatga tttctgaagt
     9301 gcctgtagtc atcaatagta acgcagtaag tggctgctgc agatctgtgg caaatcctgg
     9361 ataaggatag gtcatgatgt ttgcgggttt taattctttc gaggggtaaa cttcgatttc
     9421 atcttcgctg attttcatat ggacaccaat ttcctgcaat tttgctataa agctttccaa
     9481 atgttcaaag atcacatttt tcactttgat accatttcct actgcagctg ctaatgctag
     9541 ataggttcct gcttcgatgc ggtcaggaat catgaaatgc cgacagccat gtaatgtttc
     9601 gactccttcg atacggatca catctgtacc cgctcctcga acttttgccc ccatattatt
     9661 cagtaaagtt gccacatcta tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg
     9721 tccttttgct tttacagcag ctaacatcac attgatcgtt gctccgactg aaaccacgtc
     9781 catgaaaatc cgattgccac gcaatccttt attttctgtc cgtaaataca tggcaccatg
     9841 ttcatttgtc acttcagcac ctagagcttc aaatcctttg acgtgtaggt caatcggtct
     9901 agggcctaaa taacagccac ctggcaggcc tactaccgct tcgccaaatt taccaagcag
     9961 tgttcccata aaataataag aagcacgcaa actattgatt tttccgtgcg gcatcggaat
    10021 cgatacgact tctcgaggat cgatttctag tatgttgttg gaaaaatgga cagttgcgcc
    10081 catgatctct aaaatttcaa ttaatgaatg aacatcttgt atgtccggca caccttccaa
    10141 gatcactggg gaatcagcta agattgcagc tggaatcaat gcgactgcac tattttttgc
    10201 accgctgatc gttacttcac cagctaaagg tcggtttcct ttgattacga tttttttcat
    10261 ctgtttccac cttgtttatt aatctttttt catggatatg cttgtatttt atcatagata
    10321 aaaaaaaaga aacaagcatg gtctgccgtt ttaagtaaaa aagaatggag agcgacttca
    10381 aaagtcgttc tccattccta tatatcgtat ctgttatgtg gatgcttacg ctttgttagc
    10441 tgaaccgaac cagtcgatat gagatttagc atcttcaacg attgctgctt tacctggagc
    10501 taataattta cgagggtcga atcctttacc ttctttatct ttaccagctt cgatatattt
    10561 acgagtagct gcagcaaatg ataattggaa ttcagtgtta acatttactt ttgagatacc
    10621 cattgagata gctttttcga tttgatcttg aggaatacct gatccgccat gtaatactaa
    10681 aggcatgtct ggacctacag catcagcaat tgcttgtaag tggtcaaaag ctaatccttt
    10741 ccagttttct ggatattgac catggatgtt accgataccg caagctaagt agtcgatacc
    10801 tgtagcaacc atttgtttgc attcttcaat atcagctaat tcgccgttac cgatgattcc
    10861 gtcttcttct ccgccgattg aaccaacttc acattcaact gagatacctt ttgcatgtgc
    10921 tttttcaaca acgtctttag ctaatttcaa gttttcttca aatggtagat gtgaaccatc
    10981 gaacattact gaagtatagc ctaattcgat acattccaaa gcagcttcgt aatcaccatg
    11041 gtctaagtgg atagcaactg gaacagtgat gttcattgct tcaactaaat cagtgattaa
    11101 atctttacag attttgtaac cacccatgta tttagctgca cccattgatg tttggataag
    11161 tactggtgct tgttttgctt cagccgcttc aaggattgct tgagtccatt ccaagttgtt
    11221 tgtgttgaat ccgccgacag cgtaaccgcc tttacgagca gcttttaaaa attcagctcc
    11281 tgatactact ggcataataa aattcctcct aagttttgta aaactaaatt tgttaagaca
    11341 attcttaacc aacgacccta ttttaccaaa catttcggta gttttctact aaaatcaatg
    11401 aaaatgaaaa ttttcaa
//
LOCUS       DAZKSR010000097        10918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000097 DAZKSR010000000
VERSION     DAZKSR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.098"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..561
                     /locus_tag="ACG8XG_002385"
     CDS             <1..561
                     /locus_tag="ACG8XG_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0774273.1"
                     /translation="PVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKI
                     DYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNS
                     KYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKE
                     DYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            596..1363
                     /locus_tag="ACG8XG_002386"
     CDS             596..1363
                     /locus_tag="ACG8XG_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0774274.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1555..1953
                     /locus_tag="ACG8XG_002387"
     CDS             1555..1953
                     /locus_tag="ACG8XG_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0774275.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2260..2634)
                     /locus_tag="ACG8XG_002388"
     CDS             complement(2260..2634)
                     /locus_tag="ACG8XG_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774276.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2656..3006)
                     /locus_tag="ACG8XG_002389"
     CDS             complement(2656..3006)
                     /locus_tag="ACG8XG_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774277.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(3023..3418)
                     /locus_tag="ACG8XG_002390"
     CDS             complement(3023..3418)
                     /locus_tag="ACG8XG_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0774278.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3485..3670)
                     /locus_tag="ACG8XG_002391"
     CDS             complement(3485..3670)
                     /locus_tag="ACG8XG_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFQ0774279.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3752..4006)
                     /locus_tag="ACG8XG_002392"
     CDS             complement(3752..4006)
                     /locus_tag="ACG8XG_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774280.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(4028..4273)
                     /locus_tag="ACG8XG_002393"
     CDS             complement(4028..4273)
                     /locus_tag="ACG8XG_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774281.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4280..4564)
                     /locus_tag="ACG8XG_002394"
     CDS             complement(4280..4564)
                     /locus_tag="ACG8XG_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774282.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4667..4849)
                     /locus_tag="ACG8XG_002395"
     CDS             complement(4667..4849)
                     /locus_tag="ACG8XG_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0774283.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(5055..5540)
                     /locus_tag="ACG8XG_002396"
     CDS             complement(5055..5540)
                     /locus_tag="ACG8XG_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774284.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVDTKVTKIYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(5707..5973)
                     /locus_tag="ACG8XG_002397"
     CDS             complement(5707..5973)
                     /locus_tag="ACG8XG_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0774285.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5975..6196)
                     /gene="relB"
                     /locus_tag="ACG8XG_002398"
     CDS             complement(5975..6196)
                     /gene="relB"
                     /locus_tag="ACG8XG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0774286.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6422..7240
                     /locus_tag="ACG8XG_002399"
     CDS             6422..7240
                     /locus_tag="ACG8XG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0774287.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            7253..7540
                     /locus_tag="ACG8XG_002400"
     CDS             7253..7540
                     /locus_tag="ACG8XG_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774288.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7722..8075)
                     /locus_tag="ACG8XG_002401"
     CDS             complement(7722..8075)
                     /locus_tag="ACG8XG_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774289.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(8095..9150)
                     /locus_tag="ACG8XG_002402"
     CDS             complement(8095..9150)
                     /locus_tag="ACG8XG_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774290.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9366..9764
                     /locus_tag="ACG8XG_002403"
     CDS             9366..9764
                     /locus_tag="ACG8XG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774291.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9767..9940
                     /locus_tag="ACG8XG_002404"
     CDS             9767..9940
                     /locus_tag="ACG8XG_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774292.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3635 a         1679 c         1859 g         3745 t
ORIGIN      
        1 cctgttttct ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct
       61 tttcgagact ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact
      121 gattttaaaa ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc
      181 ttgcttacac gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct
      241 tatttaaaag gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg
      301 aatagcaaat acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc
      361 gatcttaaac ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc
      421 aaagatagag gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag
      481 gattacacaa taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct
      541 tatgtgaacc tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa
      601 aaaagatgaa ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga
      661 tttaaagtcg tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa
      721 gaaaacaggc gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca
      781 aaatcttgaa acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga
      841 aatttcgaat ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat
      901 tcattgggat ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt
      961 aattgtctca atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca
     1021 aaaaaaatat agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat
     1081 cgccaaaaat aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat
     1141 agttaaaatt cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga
     1201 tagacgtgtt ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga
     1261 ttttgctaaa gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag
     1321 tgagtttgat gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac
     1381 tcgtttgaag atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta
     1441 atctccttta gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct
     1501 tttttttgaa acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa
     1561 tacttttttt gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat
     1621 tttccagact tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa
     1681 gtattggaag gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg
     1741 cctgttcaag agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta
     1801 aaagctaata cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg
     1861 actgtggaac actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct
     1921 ttgaatgatt tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa
     1981 aaacagcttg ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa
     2041 aagctctcag aagccttctg agagctttta aattgctatg tattgtactg catctgctca
     2101 aattgatttt cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata
     2161 tgtattgagt aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat
     2221 gtgttaggat aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt
     2281 tttatcggta ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat
     2341 ccctgcatcg ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat
     2401 caaaatggtg tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga
     2461 cttataaaaa agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta
     2521 tttccaaaga tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc
     2581 tgctaattga tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct
     2641 cttctcctgt ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc
     2701 ggtttgaaat tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga
     2761 ttcacttaat gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt
     2821 atagcagaat tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa
     2881 gttattatcc tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc
     2941 agttgtatct tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc
     3001 tttcataggt tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa
     3061 gtagttgaga gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac
     3121 taacattttt tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt
     3181 ggatgattac taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag
     3241 gaaattctgc atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat
     3301 atgcttcttc aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt
     3361 tataagcttc tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt
     3421 cctcgctcct tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag
     3481 gctgttaaag ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat
     3541 tgtggatcgg aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc
     3601 cagtttgtcc agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg
     3661 tcttaggcat agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat
     3721 caatagaaaa agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag
     3781 caaattgaat gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat
     3841 aggtatctaa gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt
     3901 tctccaagtc ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta
     3961 tgaaaacata ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt
     4021 tcttgtacta agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata
     4081 ggacgtgacc gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa
     4141 ggctagtacc tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg
     4201 gatcaatgcg agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt
     4261 ccaaagtcca cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt
     4321 caatagtcac ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca
     4381 agttcccgat gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca
     4441 cttgccgact gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg
     4501 cattaattgt ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta
     4561 gcatcttgtc actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc
     4621 ggtgtgtata atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa
     4681 acgataaagt aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg
     4741 ctttgcttgt gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc
     4801 cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc
     4861 cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat
     4921 ggtgatgttt ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt
     4981 acctaatacg tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt
     5041 tatgtattta agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga
     5101 gtgagtgatc catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca
     5161 gcgacatacg atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt
     5221 tttggtttat tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga
     5281 tcaccgacaa agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca
     5341 tttaagaagt agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag
     5401 gttccttctg catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg
     5461 atttccaaac catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa
     5521 taatcaccat accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca
     5581 cttatattat acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa
     5641 gtgggatcgt aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct
     5701 tgtctgttac ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc
     5761 atgctcgcta atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga
     5821 tttattagct gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt
     5881 ctcgatccag tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg
     5941 agcttcacgt tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat
     6001 cttcgtggga aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg
     6061 caaagtctac taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt
     6121 tatctccgtt gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa
     6181 tacttattac tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg
     6241 acaaatgcaa tagttatata tttgaatata tacataaaca taaacatata catatatatg
     6301 tttatgttgt agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata
     6361 tacataaacg tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat
     6421 catggaaaag aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac
     6481 aacaacaatc tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta
     6541 tattgatgaa gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc
     6601 gaaaagagag aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat
     6661 aaatgagaac atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat
     6721 gcgtgatatg cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca
     6781 gcttaaagag aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc
     6841 tcaaaatgct tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg
     6901 ttttgaagct ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga
     6961 tattaagact ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa
     7021 acataatact aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct
     7081 tggatatgta caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc
     7141 tgagttcgct aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt
     7201 tacgctatac aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga
     7261 aaatagattt aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc
     7321 agttgaagaa cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa
     7381 aagtgatagt tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa
     7441 gaaattatct aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga
     7501 aatgatagat aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact
     7561 ttttttattg gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg
     7621 atatatacgt atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac
     7681 ataataaaaa ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta
     7741 cttggtgcat tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta
     7801 accgttgaag gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct
     7861 gctactttgc tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt
     7921 acacgccgat ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt
     7981 tcatattcgg attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt
     8041 ataactacat aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg
     8101 agttaaacca aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa
     8161 ggtatgcgga attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt
     8221 aaagacaact ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac
     8281 catattggtg tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt
     8341 tccgcttggc ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag
     8401 ttggaaagca ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt
     8461 aaaaatgccg gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac
     8521 atcaaaaagt ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt
     8581 ttcatcttgt acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg
     8641 tggcagtacc caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga
     8701 atgttgtcgt gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga
     8761 aggcagacct aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc
     8821 taggttagct aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat
     8881 cccaaaacct agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc
     8941 aaaataatag ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt
     9001 taatgaggaa cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg
     9061 agagggaagt gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa
     9121 gtcaacggaa aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg
     9181 tatttcggta cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa
     9241 aaaatgcaat cataaaagtc taattaatta taataaagtg taaataaaaa ggtgtaaata
     9301 aatgcttgca aatttatctt attatgatat tataaaatca taaattatat taaggagtga
     9361 tcctgatgcg attgagaaga aataagaatt acactaccat tattttagaa gattttactg
     9421 atcaagaatt acttattgtc ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt
     9481 caatagtaaa agcttcaaag gttgctgtcg aaaaatattt tgattcttta gaagaaagtg
     9541 ctaaggaaat ttttaatgga gagaaagctg gtagctatga gttagacggt attagattta
     9601 gcttgatccc aacaactgaa aatattattc cgaagaaatt atattatatc ttttcatctg
     9661 aaaaacatac ttcaatgaag ccttgtattt ttgattggca atggttgatt gatgctgtat
     9721 tggatgtaaa atgttcaaaa tacaatatta ctttggggtg ttaattatga gtatgctagt
     9781 tagaaaatta ccattatctg taagagaatt tctattttac ttggtctgct taatcgcatc
     9841 agggtttaag ctgatcgaac atatttttat ctttgcttat gtattagcaa tgatcgcatt
     9901 tttatctggc ggttggttaa ttatttattt gttaagctaa gttttagatc aaaaataatt
     9961 agtaaattaa acgataaaat aaccgttaat aaatgtaatg aaaggacaac gcctaacaat
    10021 cagcttgtaa taagattata aaataacata atatcaaaaa tagtgctttt taaaggacaa
    10081 attaagtgta tcaacataaa cgtgtatatg tatatgttta tgttgatact ttttttgtct
    10141 taatttttta tttttaaaat tctatttact aatgctgtat agatagtgat cagaaaaaac
    10201 aaaatagcga gattaagcta tttattagtt catatattat attcagatta atatgatcca
    10261 gtattaatct gaaggcaaaa ccaatatatt tctacggact cttgtaaaac tatttaaatt
    10321 ttcctgctaa taaaataacg aaaatagaat atttagcttt tttataccat tttacgataa
    10381 catgatttca tttgtaatat taaaacattt aaatttttaa ttattctttc aaattagatc
    10441 aattaatatc cttataaaag agcataataa atggttttta catcgttatt tattgatatt
    10501 aatctcatcc aaaaaaaatg aaaacgcata cttttatggg tgattgaatg tgattttaat
    10561 acatatacca attaaaagaa aactttttag atttttactt acaaatttat aaaaaaacaa
    10621 gataattgat actttgattt tgcgaacatt ttatcttgcc gcctaattca gctttaatat
    10681 catgattata tcagtaacaa atcgcttttt tagctaattt aagcatcaaa taattattct
    10741 taatcaggga aaaaataact aataaatgga ttttgggaat aggtaaaaac ttgatcggtt
    10801 tctggctaaa aataacgtga aaaaggaaat ataaaataac atggagcgtg ggaggaataa
    10861 agaaatttta cacgaaaggt gcgcagggat gtaaagaaac ttaacacgaa acaaaagt
//
LOCUS       DAZKSR010000098        10658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000098 DAZKSR010000000
VERSION     DAZKSR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.099"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8XG_002405"
     CDS             33..656
                     /locus_tag="ACG8XG_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774293.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8XG_002406"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8XG_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0774294.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8XG_002407"
     CDS             2152..3033
                     /locus_tag="ACG8XG_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0774295.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8XG_002408"
     CDS             3036..3806
                     /locus_tag="ACG8XG_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774296.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8XG_002409"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8XG_002409"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0774297.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8XG_002410"
     CDS             5117..6280
                     /locus_tag="ACG8XG_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0774298.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8XG_002411"
     CDS             6397..6807
                     /locus_tag="ACG8XG_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0774299.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8XG_002412"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8XG_002412"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0774300.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8XG_002413"
     CDS             9380..9664
                     /locus_tag="ACG8XG_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774301.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8XG_002414"
     CDS             9824..10060
                     /locus_tag="ACG8XG_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0774302.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8XG_002415"
     CDS             10057..10455
                     /locus_tag="ACG8XG_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0774303.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3724 a         1773 c         2253 g         2908 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatat
//
LOCUS       DAZKSR010000099        10088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000099 DAZKSR010000000
VERSION     DAZKSR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.100"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..801
                     /locus_tag="ACG8XG_002416"
     CDS             322..801
                     /locus_tag="ACG8XG_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0774304.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            834..3062
                     /locus_tag="ACG8XG_002417"
     CDS             834..3062
                     /locus_tag="ACG8XG_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0774305.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3191..4834
                     /locus_tag="ACG8XG_002418"
     CDS             3191..4834
                     /locus_tag="ACG8XG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0774306.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4907..5452)
                     /locus_tag="ACG8XG_002419"
     CDS             complement(4907..5452)
                     /locus_tag="ACG8XG_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0774307.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5475..5960)
                     /locus_tag="ACG8XG_002420"
     CDS             complement(5475..5960)
                     /locus_tag="ACG8XG_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0774308.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5950..6762)
                     /locus_tag="ACG8XG_002421"
     CDS             complement(5950..6762)
                     /locus_tag="ACG8XG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0774309.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6901..8433)
                     /locus_tag="ACG8XG_002422"
     CDS             complement(6901..8433)
                     /locus_tag="ACG8XG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0774310.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8542..9573)
                     /locus_tag="ACG8XG_002423"
     CDS             complement(8542..9573)
                     /locus_tag="ACG8XG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774311.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3361 a         1857 c         1939 g         2931 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaatac aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagccaaaa
      121 tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat
      181 ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta
      241 taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt actaaaaata
      301 aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc
      361 gttgtagaag actatgataa agttgatgga agaaatgcat taaatatgga tgcaaaaggg
      421 ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta
      481 tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt
      541 ttagatttag cgatcttagc tgcgcaagga agtttatact tccaagatgc ttatcgttat
      601 gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga
      661 atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac
      721 gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt
      781 ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga
      841 atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga
      901 aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga
      961 attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg
     1021 ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga
     1081 cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt
     1141 ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag
     1201 ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat
     1261 tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt
     1321 acccttctga aatcgttcga gcgattgaaa aaggctattt attagagatt caagagccga
     1381 ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga
     1441 tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta
     1501 caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac
     1561 atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa
     1621 ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg
     1681 aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg
     1741 caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata
     1801 aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat
     1861 tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc
     1921 atacaaaaga aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg
     1981 ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg
     2041 gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag
     2101 aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc
     2161 aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata
     2221 aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc
     2281 gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa
     2341 tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa acagagacgt
     2401 accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt
     2461 tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac
     2521 ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag
     2581 cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata
     2641 cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg
     2701 gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg
     2761 gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt
     2821 tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa
     2881 aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg
     2941 cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag
     3001 atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat
     3061 gataaaataa agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta
     3121 ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga
     3181 gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta
     3241 tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga
     3301 aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca
     3361 atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt
     3421 cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt
     3481 gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa
     3541 aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa
     3601 agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac
     3661 tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga
     3721 gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg
     3781 atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga
     3841 gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa
     3901 acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga
     3961 agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca
     4021 tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacatgc
     4081 attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa
     4141 ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat
     4201 gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca
     4261 aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt
     4321 agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt
     4381 ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga
     4441 acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac
     4501 gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat
     4561 gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca
     4621 tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat
     4681 ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg
     4741 gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc
     4801 ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt
     4861 ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc
     4921 taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg
     4981 ttttcaaacg aggtctgaca ggtacttgga catgcttgat atgcataccg attgatgtgt
     5041 caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact
     5101 ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa
     5161 tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt
     5221 cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga
     5281 tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc
     5341 ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt
     5401 cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa
     5461 accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata
     5521 ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact
     5581 ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag
     5641 cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca
     5701 acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa
     5761 atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa
     5821 agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt
     5881 acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc
     5941 gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag accagacact
     6001 tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact
     6061 ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga
     6121 aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc
     6181 ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc
     6241 tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc
     6301 tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt
     6361 aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat
     6421 cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt
     6481 tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt
     6541 gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa
     6601 agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc
     6661 gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc
     6721 agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa
     6781 taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat
     6841 ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag
     6901 ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc
     6961 tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg
     7021 gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg
     7081 ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc
     7141 taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg
     7201 atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca
     7261 atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc
     7321 tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc
     7381 tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt
     7441 tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata
     7501 tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg
     7561 gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg
     7621 ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg
     7681 ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat
     7741 aggaaatccc atggacatca aagaagagat accaatattg aatctggaat gcactggaga
     7801 gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt
     7861 cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc
     7921 agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa
     7981 agcaggttgt tccattaaat aaagaatagg aatctgtttt gattttgttc catttgaaat
     8041 ttctctcact cgcttttttg tctcatcatc aaataaaatg aaagggtctt ctaaacgatc
     8101 gataaatact tccattgttg ttgttggtag atgataagaa gtcatgacag gcactgcacc
     8161 aaggtaagac acagaaacag ctagaaggta tgtgtcaaaa gcggaactct tataaataat
     8221 aactttgtct ccggatttca ctccaagatg agccaattga taagcccgtt tcaacacact
     8281 ttcgcagcat tttttgtaag ttgtttcaag tcctaattct gggaaagctg aaagtggctc
     8341 atcaaagata atcggggtct cggaaaagtt agctgcagct tcttgaaaat tcgtaaataa
     8401 gttcaaaggg gaatacgtta tggtctccat cattcacact ccattatctt ttgttaagta
     8461 atatacaaat tcaagataat cgtaacataa tcctttatat aataacatag tttattgtga
     8521 aaaaaagatc atacatttgt tttatatgga agaaacccga acgaatgccg ctaattcgtg
     8581 ataagaaaaa tcaaccatct tttcccagat atcttgaaaa gaatgggatg agaggtatcg
     8641 attaccacat aaataattta ccgcgtcagc atcatctaaa tatagttctg cataataacg
     8701 atatgttttt ctgcatagtt ctggcgatac gtttttatat actgtaattt tgctcaaaat
     8761 ttggcgaata gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa caaaagaatt
     8821 ttcgtccgta ttttgtgctg ttaacttaaa atagttctcc agaaatggca tcgcttcttt
     8881 tggtattggt actttattca gtctgttttc ttttctattc agattaagaa tctgcttggt
     8941 caaagataca tctttacgtt taagttctac tacttcatgg atctttaatc cgcaatacag
     9001 aagaatggca gtaattgcaa ggttcctcgg tctattcttt tcttcaaagc tagcttctct
     9061 gcttgtttga aagccttcaa tcgggataaa caaatgctca aaatctttga aaagttctgc
     9121 atcgatatat cgcggagctg ctttggttgt ttccggtaaa cttgtaccca aaagagaaat
     9181 ctcttcttca atcagtcctt tttgatacaa aaatcgtaaa aaatgattta atgaatattg
     9241 cttccttgaa atcccgtaat agctattttc aaacaattgc tccgtttctt ggttcgctgt
     9301 agtttggaac gaacgagtat gtcgatagat tttagctaag aaattctgat aatcttgttt
     9361 ttctaaatgg ataatcagct gatcatccac ttcttcagaa aatacttttc tcaaataatc
     9421 agtaaacaga tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa gggaagaacg
     9481 attttcttct aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt gctttaactt
     9541 ttcttgcagt tgttttctag ttttgctgtc cattacaaat tccctccaat agtgttcatt
     9601 ttctatattt taccgcattt catcaaatga agatagacag tttgtcttga tcttaggaag
     9661 caaattttta actaagtact agctaaaaga tatgaagtaa gaagatacat ttttagttat
     9721 tttattagtg cagtcattct tttgatcttt taattgattt atcctctttt tagctatgtg
     9781 gttacttcat gagcaatgaa atggatttgg ttcgttctaa tggcaggagg ctctttcttt
     9841 tatttccgaa aaattagaaa acattttttc agaatcgatt ggctctccat ttattcaata
     9901 atggtttgtc taacgatatt gttgggataa ttcttaaaga agtattagaa atagttggta
     9961 cttcattact ttacatgaag atttattcta tggtaggagc aattatattg agaattaatc
    10021 cttttttata taagttgttt gcttattgat ggattggata aattaaacca tcacaacgag
    10081 tggaaatt
//
LOCUS       DAZKSR010000100        10007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000100 DAZKSR010000000
VERSION     DAZKSR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.101"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..2153)
                     /locus_tag="ACG8XG_002424"
     CDS             complement(33..2153)
                     /locus_tag="ACG8XG_002424"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0774312.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="ACG8XG_002425"
     CDS             2383..3561
                     /locus_tag="ACG8XG_002425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0774313.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="ACG8XG_002426"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="ACG8XG_002426"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0774314.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="ACG8XG_002427"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="ACG8XG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0774315.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="ACG8XG_002428"
     CDS             complement(4914..6167)
                     /locus_tag="ACG8XG_002428"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0774316.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="ACG8XG_002429"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="ACG8XG_002429"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0774317.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="ACG8XG_002430"
     CDS             7745..9091
                     /locus_tag="ACG8XG_002430"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0774318.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="ACG8XG_002431"
     CDS             complement(9399..9785)
                     /locus_tag="ACG8XG_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0774319.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9834..>10007)
                     /locus_tag="ACG8XG_002432"
     CDS             complement(9834..>10007)
                     /locus_tag="ACG8XG_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0774320.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3192 a         1821 c         2040 g         2954 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactagt gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttgtctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat tatacccgtg gtgctagtta attttaatat
     9841 cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg
     9901 taagccagta atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa
     9961 ttttgacaac ctaatttttc taaggaagaa aaaacagtct caactgt
//
LOCUS       DAZKSR010000101         9963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000101 DAZKSR010000000
VERSION     DAZKSR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.102"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1148
                     /locus_tag="ACG8XG_002433"
     CDS             105..1148
                     /locus_tag="ACG8XG_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0774321.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="ACG8XG_002434"
     CDS             1321..1545
                     /locus_tag="ACG8XG_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774322.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="ACG8XG_002435"
     CDS             1548..1805
                     /locus_tag="ACG8XG_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774323.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1907..2260
                     /locus_tag="ACG8XG_002436"
     CDS             1907..2260
                     /locus_tag="ACG8XG_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774324.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2313..3107
                     /locus_tag="ACG8XG_002437"
     CDS             2313..3107
                     /locus_tag="ACG8XG_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774325.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3136..4254
                     /locus_tag="ACG8XG_002438"
     CDS             3136..4254
                     /locus_tag="ACG8XG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774326.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4287..4607
                     /locus_tag="ACG8XG_002439"
     CDS             4287..4607
                     /locus_tag="ACG8XG_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774327.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4862..5551
                     /locus_tag="ACG8XG_002440"
     CDS             4862..5551
                     /locus_tag="ACG8XG_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774328.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5588..5755
                     /locus_tag="ACG8XG_002441"
     CDS             5588..5755
                     /locus_tag="ACG8XG_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774329.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            5775..7403
                     /locus_tag="ACG8XG_002442"
     CDS             5775..7403
                     /locus_tag="ACG8XG_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774330.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7454..7825
                     /locus_tag="ACG8XG_002443"
     CDS             7454..7825
                     /locus_tag="ACG8XG_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774331.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7910..8110
                     /locus_tag="ACG8XG_002444"
     CDS             7910..8110
                     /locus_tag="ACG8XG_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774332.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8244..9470
                     /locus_tag="ACG8XG_002445"
     CDS             8244..9470
                     /locus_tag="ACG8XG_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774333.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
BASE COUNT         3903 a         1197 c         2004 g         2859 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagtta cttttctcaa
     1621 ataagtacac cgcaaaagag ctggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaagggatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggac ttcgatccta taccacttct gaaaaagaca
     1801 aataacttaa tgtttgaaaa gataaaatat ctgtcttaaa gctatgtcaa attcaaatgg
     1861 tgaatgtttg ctaaatataa tgcaaaaaaa ggaagggaaa tattaaatga taagcgaaaa
     1921 tttgtcttat gagaataata aacatctagt taaagaaaat aagaaagtag aagattataa
     1981 gaacatgtat atagaaatga aaaaatcacc actgtgtatt caatataaaa atgataattg
     2041 gaagcaagta gtcgaatttg taaaacctca tagtatacga agagtacgaa gggggaagaa
     2101 aaaaagtgaa cttgtaaata gcggaagagc ggtaattaga gttataacca aagattatcc
     2161 agttgaatat ggagattata ttttaaggtt aagttatcat gcttttgttg tgttgaaaga
     2221 agagcatttc aacgagtttt tcaatttaaa aagtaagtaa tcatattttt ttgtagacgt
     2281 gtataaagtt aactaaattg cgaggtgatg acatggtagc aaaaaagaat actctaaaag
     2341 aagcttctga aaaatatcaa gatatgaaaa gacgaggatt aataccgaaa gacaaaaaag
     2401 gtatatggag agaatttgtt aaggaagtca gtagtgatcg aagtggcggt gactttgttg
     2461 gacgatatac agcaaaaggc tatagcaaac gattaaggat cactgttgaa tatggaacga
     2521 atgaatcagc tacagagctg aagcaaaggt acttagatac acgaagggaa gcttcaaaaa
     2581 aatataatga tttcaagagc agaggtctga ttcctaaaaa gaaaaaggga atttggcgtg
     2641 agtttttcaa ggaagaagat agcaatcgga ataagggaga cttcattgga cgatacacag
     2701 caaaaggcta cactaagaat ttagggatca aggttgaata cggaactggt gaaagttcat
     2761 gggaattgag aaatcgctat ttaaatacat tgttagagaa atataaagca gaattattgg
     2821 agttgttcga tgttaatgat tttatcgaga tggctgaaaa gtatggtcat gaagaaggtt
     2881 ttatgatcaa tgacattatg gttattgata attttacgga tttattagca cttgcgaaaa
     2941 aagcaaggga tttaagagaa acgcagatgt atctattcag aaataaaaac ggaagaaacc
     3001 ggagaactga atcgagtgag attgaagatt actacgatgc ctttacacac gatctgggag
     3061 cctttgtaga cgagataagt ggtgtaatga acaaatatag ggtctagaat aaaaaagccg
     3121 gagatgagca gataaatggg aaacaatagc atatacacac ctgaaatgca gaataaaatt
     3181 aaaagacttg tcggattagg gtggatgaca agaggaaaat tagattcttt tagtacgatc
     3241 aaatatcttt tgatggacat ttaccgtcag aacaagcaaa caggaataca ttttttgggc
     3301 tatgataagc cggaagatgc acctttcttt gttccaagtt cagcctatag agccagtacc
     3361 tccggtagca tgtataactt ggatttgttt gaggaaggag caaagatgat cgaatatgat
     3421 ttcaacgaag cctattcgag gatcatgaga aattatcctt tgccgtcaaa cacttatttg
     3481 aaagcaaaat ataatacaga tcaagctatt ttgagagcga taaatagacc aaaagtaaga
     3541 ccgtatgaaa atttaagaac gtatatgttc tttttggtta attttgaagg aacagccaaa
     3601 gaaggaacat atgctaatcc aaaatcacag tcgatgtttg cggattatgg cgaaaatccc
     3661 aaaacagcta atgagcctat ttggttatcg gagattgagc taacacttat ccttgattat
     3721 tatgatatta aagagatgga agtcctagat agccacacat ttttgacgaa aaaaggatta
     3781 ctcaaagaat attttgaaag aatcgagcca ttaaaaactg atcctgatac gttgaagctt
     3841 tataaaaatc ttagaacgaa agtatttggt gctatcggac aaaaatcatt aagagaagga
     3901 gaaacaagca ccttcgataa atacagtatg tataatcgag cgtattcctc catggtagca
     3961 ggcgtattta gagatcgtat ggttagatat gaacagaaat acgttcatag cgcatatggg
     4021 ctattaaaaa ttcggactga tggattgtat ttcagatccg aagtaccaga gtttgaagag
     4081 ctgtataaac ttggagtagt taagaaaaaa gttcatacaa tatccaaaga agaagtcgaa
     4141 aaagctttag aatttaaaaa caaaggattt gaaaataagg gctcctttgg gaaatctaaa
     4201 gatgaagtta agggtgttgt gatagacgat gatttcgagt ttgattttga ctaaatactc
     4261 tttggaaaag atggtaggag gtaaatatga agaagaaaat tgtggaagga aatgtaggtg
     4321 ttattattct aaaagattca actgactatg ctaaatcaat ggcactaaaa gacgaaaaag
     4381 ttgcacgcat ggataaagat ttagttgaat ttctgatgga tgatacagaa agattaaaag
     4441 gggatcagtt tttgagttta atgaaagaca aaggcttaaa cacatatggt ttatttgatt
     4501 ataagaaatt agctatagag tgggtaaaag aaggagaaat attttttatt gaagaatggg
     4561 aaggtactga gaacttagtt cttcatcagt atcttttgaa agcttaatct aagtaaaaca
     4621 gttctgacta gttatgcttg tcagaatttc aaaggaagtt ttccataacc atcatcattc
     4681 aaaaataaag gatttgaaaa taagggttcc tttgggaaat ctaaagatga agttaagggt
     4741 gttgtgatag acgatgattt caagtttgat tttgactaga tttttttgaa aggtttaaga
     4801 gtaaacaagg taagaactaa gatcatgaag attgagagta aagaagatag aggacgaatg
     4861 tatgagaaca cactatattt atttagaaga taaaattgta gatgaaattc cggcaaaaag
     4921 tcgagaacaa gctagaaaat tatttattga aaaatacaaa gagatcaatg gagatagtga
     4981 agttccaaaa gtggtcgtaa cagttagaaa aaggcaaaca aaaaagagaa gtacgtttca
     5041 tgtcagccct gatatatata atcttataga gcgacataga agtgaagata atatttctat
     5101 ctctcaatat attgctacag ttattgatga attgatttca ggaaagtatg actatgcttt
     5161 tgattttgag ggtgaaaagg attacaaaag tgttgctgtc tttcaatctg attttgagaa
     5221 gctaaaaaac tatgctggca ggagaaatgt taattttgca agactactag aagagttcta
     5281 ctccgaagag ttgggagtag gaaatgaata caagagagaa acttatgcga aattgtctca
     5341 taagttatcc caagcgctta aagaagtgac agaagttaaa gcggaactag atgctaaact
     5401 tgaagaagtg aaaaaatatc aaacagagag gaagaacaac atgaagaatc aagtggaatt
     5461 tgacgaacag aaagaatttg aaaaagcaag aaaacgctat aacaaaatta tgaatgaact
     5521 tagctatgaa cacttaacag ttcatgaata attgagtgag ggtacagaaa aatatacgat
     5581 cgcagatatg gttaatgaag tggaaactct tttagcttct ttctttgaaa atggtcacgt
     5641 aaacgatgaa tggcgaaaag aagaacctaa aagggttaac gcttatattg ctcgattaaa
     5701 aaggtttatt gataaatata aggagaatag tgacaaattt tctaaagata aataaggaga
     5761 gagtgtgatt acatttgcca gaaatcgaag gaatattttc gccaaaaata ggtaaaaaac
     5821 agttagattt cagcagtttt ccgagcgtaa atagagatta taatctaggt gatgaggtac
     5881 agatactaaa tattgatttc aaagaactag acttcgctgt tgctgtttcc aacaaaccca
     5941 aggcattgga acgagtgaaa aaaatatacc aagagaatga tcaaaatatc aaaaatatga
     6001 tcaaaaagta tagacgagat aattcaaaag ttgaagacgt tttattcaac ggtacggcaa
     6061 aacatgattt gaatgtttat atggcattag gagtggcttt gtactgtaat tcaattaatc
     6121 agacagatat tttggttaat atcgtgagga atagctatca aaaaatatta aaaataagta
     6181 gcgaaacaaa cccttattat gcaatgaaat atcttgggga taatttatta aatgataaaa
     6241 aattgatgca caacactgaa gagctgttat ctaatttggc tgcgattaac tttctattca
     6301 aaacaggttc tgaagattat tttatagata acgtactaaa aggtgcttac ggaacagcat
     6361 tttatccgct ttacaatatg aaacagcata aagccgaaca aaaggataac aaacttgaaa
     6421 ttaaaaagat gaaagaacta ttaggtatag gatttgtaaa acccaattca aaagtcacta
     6481 ttgcttcgtt agcaactact aacaatgatg ggaaaatagt aaatgggttt gacgattcag
     6541 atatagatat attgtcaaaa tcatattttt ctttgtatgg agattttgca gatttgatgg
     6601 gtattgatag ttacttagtc aataattttg agatcaaggg tagcgagctg aatgatattg
     6661 tgtatttcgt tttaatctct ctatatgcaa ttgatcagaa taccgagtac cgacaaattg
     6721 actatttgct agttttttat agtttgttat atcagttcgt tttaactcgg ttgtttaaaa
     6781 atgctaagga agaattgatc ctgaattctg attatgaaat agcaaaacaa attgcaagta
     6841 ttagaaaaga aaacaaatct gaagtaatca aattaagaaa agaattaagt attgcgagta
     6901 aagaaaacgg tcggttagaa tctttagtca aatcggcaaa aaaagaagag gaacttttga
     6961 agcaagagat taaagaatta aaaggaatca gaaaagaact tgaacagctg aggtttgaaa
     7021 ataaagtatt aaagcaattg caggagcaag aacatagaca atcattaaat ggtaaaactg
     7081 aagagccttc actgtctgaa atgatagctt atctaaaaca taaaaacatt cttttagtgg
     7141 gaggacatgt aaattttcaa aataaattaa ctgaactatt accaaatatt aaaatgtttg
     7201 atgtaagcga aaataatgga aagatactta aaaatttgaa tacgtgtgat gcgatttttc
     7261 tatatacaga ttacttgaat catggtctat attatcgtgt tatgagtgaa atttctaata
     7321 aagataagat attttatttg gataatcatg tgaatatgaa cttaacaatt aaacgtattt
     7381 acgatctttt atcacttata taaagagaga agtgataaat aggacacaaa atttggaaag
     7441 taggtaagtc actatgagag aactaacttt atcttctaag gaattagaaa agaaaactgc
     7501 tttattaaac gaaatattta gtgctgattg tataatccaa agtttaaaag aaagttcatt
     7561 ttcaaaacta acagatcagg aaaaaatttc ttatatgcta agtttagata atctaggaac
     7621 taaatatgat gaatcagcaa gtagcgataa gcttttgatt gaattagtat caagtcttag
     7681 tagttttgga aaaatgaaaa gatttaatgt gatattagaa atttttgctt ggttaatccc
     7741 ttcatttatt tggttttttt atattttcat agttgaatct gagttaatag ccaaggtctc
     7801 tcttggactg ttaattccga tataaatagg ttcagcaatt tactttaata acttgtatac
     7861 acctattcaa ataaaaaagc agttattaca tgaaggatta agtggtaata tgttaaaaag
     7921 aattgcatgg gataaagata ataaaacaga tattgagctt aaagaatata aatacaatta
     7981 tttctcgaga tgtatagata gaaagttact gtttaaaaaa gaaggattag aatttagttt
     8041 tgatgaagat gaatttgaaa aattagtgat ggactggcgt aagaatttac tggatacggt
     8101 acaattctaa aagcaattga ggtcgaaatt ccgatacttg aatcatgatc tatattatcg
     8161 tgttatgagt gaaatttcta ataaagataa gatatttcat ttggacagtc atgtgaatat
     8221 gagcttaaca attaaacgta tttatgatct tttatatgtt tataaacatt aatgaggtgc
     8281 taaaattgac aacaataact atgacaaaaa aaatagaagt atatgttgat ggatcacaag
     8341 aagaaaggga ttcctttttt gatttttttt ggaaaaatag tagagaatat gtaaaagcgt
     8401 tgaatctggt atataaacag ctaatgtttg aagagttgat aaaagatgca attaaagaaa
     8461 tggatcaagg ttatgctgag agagaagaaa gtattcaaaa tcggattgaa aagttagcat
     8521 atactgatcc aatagatcaa aaaaaacttg ataaagcaaa agctgaatta tctaaattga
     8581 gaaatgaaaa aaataaagaa gcgagagaaa tattaacgca agcgataggg ttgaagagtc
     8641 aaacgagaac acgagatgta gcaaaaaaat tagactttga ctttggagat tttgttgatt
     8701 ccgcaaatat gaaagctagt aaagattttg gcaatgattt tatcggaatt gtgaccggac
     8761 aaagaacgcc tagaatatat aaaaatagaa acaaatatgg attattacct tttagaggtc
     8821 gagatattaa aatccataaa gatgataagt tttatattta tttacctaaa ggctttaggt
     8881 ttaaagtgaa tttaggtagc aagccaagaa aagcaagaga tgttgcttca gttttacaac
     8941 acattatgga tggcgattac aaattaaatc aaagtaatct tatttttgat ggaagaaaat
     9001 tattttttaa tctaacaatt tcttttgaaa aaagctataa tgaaatggaa ttcaagaaag
     9061 gtaatgaatt gatcgtaaac tttgcagatt tgtgtagcgg tgaatgttta attaatgata
     9121 aatctgtaat gtcttttggt gatagccaat atataagaaa cttcaaaagt aaaattttag
     9181 caatgaaatc aaaagaacaa agccgaggag tcttttctaa gggcggtcat ggtcgaaaaa
     9241 gaaaattaga tcaagatcga tgggaagcaa taaagaaaag agaaagtaat tttgttaaaa
     9301 cttataataa tcaatgttct gcaaaaatcg ttaaaactgc tattcgcaat aaagctcaaa
     9361 ttattcgtat cattgaacca gataaggctt atggcgaatg ggcttactat caattgaaag
     9421 aacaactagt aaacaaagga aaaagatatg ggatagaagt cagaagttag taaattaaac
     9481 aatcgttgat ttggcaacga aatagtgagg gaaaatccac tcactgaagg gtaaactccg
     9541 tcaagttgtg tgaccagacg gaaggacgaa tgttgttgag gttttagaac catataaaac
     9601 agcatgtttc tcaaattttt aggataaggg aaattgctag cagtttgttt tagaaccata
     9661 taaaacagca tgtttctcaa atgagtactt taaattaatt gatggcgttc ctgctttaga
     9721 accatataaa acaacatgga tttaaaacaa gaagtaagtc agttctcctt ttactttagt
     9781 aaggaaaaat aaagtattct caataaaact taaaataagc aaacctttgt tttaaaatga
     9841 ttgtcaagtt aaaaaaatct gggatcatat aaaatagtat agttctcaaa tatgattaga
     9901 aaccctatta aagagcaata ttaagccaac ttacttaaat cgtttaatta agaacaataa
     9961 tct
//
LOCUS       DAZKSR010000102         9873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000102 DAZKSR010000000
VERSION     DAZKSR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.103"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..267)
                     /locus_tag="ACG8XG_002446"
     CDS             complement(22..267)
                     /locus_tag="ACG8XG_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0774334.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="ACG8XG_002447"
     CDS             complement(381..959)
                     /locus_tag="ACG8XG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0774335.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="ACG8XG_002448"
     CDS             complement(1036..1566)
                     /locus_tag="ACG8XG_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0774336.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="ACG8XG_002449"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="ACG8XG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0774337.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="ACG8XG_002450"
     CDS             complement(2423..2818)
                     /locus_tag="ACG8XG_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0774338.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /gene="cysS"
                     /locus_tag="ACG8XG_002451"
     CDS             complement(2815..4227)
                     /gene="cysS"
                     /locus_tag="ACG8XG_002451"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0774339.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="epsC"
                     /locus_tag="ACG8XG_002452"
     CDS             complement(4224..4760)
                     /gene="epsC"
                     /locus_tag="ACG8XG_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0774340.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4846..5085)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5082..6542)
                     /gene="gltX"
                     /locus_tag="ACG8XG_002453"
     CDS             complement(5082..6542)
                     /gene="gltX"
                     /locus_tag="ACG8XG_002453"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0774341.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="ACG8XG_002454"
     CDS             complement(6712..7869)
                     /locus_tag="ACG8XG_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0774342.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="ACG8XG_002455"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="ACG8XG_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0774343.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="ACG8XG_002456"
     CDS             complement(9336..9863)
                     /locus_tag="ACG8XG_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0774344.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DAZKSR010000103         9802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000103 DAZKSR010000000
VERSION     DAZKSR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.104"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..501
                     /locus_tag="ACG8XG_002457"
                     /pseudo
     CDS             106..501
                     /locus_tag="ACG8XG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            579..1618
                     /locus_tag="ACG8XG_002458"
     CDS             join(579..1076,1079..1618)
                     /locus_tag="ACG8XG_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFQ0774345.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            1631..2569
                     /locus_tag="ACG8XG_002459"
                     /pseudo
     CDS             1631..2569
                     /locus_tag="ACG8XG_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            2562..2909
                     /locus_tag="ACG8XG_002460"
     CDS             2562..2909
                     /locus_tag="ACG8XG_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774346.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2906..3088
                     /locus_tag="ACG8XG_002461"
     CDS             2906..3088
                     /locus_tag="ACG8XG_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774347.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            3224..4081
                     /locus_tag="ACG8XG_002462"
     CDS             3224..4081
                     /locus_tag="ACG8XG_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0774348.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            4101..4661
                     /locus_tag="ACG8XG_002463"
     CDS             4101..4661
                     /locus_tag="ACG8XG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFQ0774349.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            5550..6317
                     /locus_tag="ACG8XG_002464"
     CDS             5550..6317
                     /locus_tag="ACG8XG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFQ0774350.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            6343..6888
                     /locus_tag="ACG8XG_002465"
     CDS             6343..6888
                     /locus_tag="ACG8XG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774351.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            6889..7455
                     /locus_tag="ACG8XG_002466"
     CDS             6889..7455
                     /locus_tag="ACG8XG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774352.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            7470..7718
                     /locus_tag="ACG8XG_002467"
     CDS             7470..7718
                     /locus_tag="ACG8XG_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774353.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            7737..7976
                     /locus_tag="ACG8XG_002468"
     CDS             7737..7976
                     /locus_tag="ACG8XG_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774354.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            7993..8577
                     /locus_tag="ACG8XG_002469"
     CDS             7993..8577
                     /locus_tag="ACG8XG_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774355.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            8684..8965
                     /locus_tag="ACG8XG_002470"
     CDS             8684..8965
                     /locus_tag="ACG8XG_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0774356.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            8955..9296
                     /locus_tag="ACG8XG_002471"
     CDS             8955..9296
                     /locus_tag="ACG8XG_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0774357.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         3589 a         1419 c         2033 g         2761 t
ORIGIN      
        1 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
       61 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
      121 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
      181 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
      241 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
      301 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
      361 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
      421 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
      481 aaatccttaa atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat
      541 aggcgtatac taaagaaaac acttgaggag tggggaacat ggaaacagaa cgccaaatcc
      601 gtgaattgga aaaagcgatg aatacgacag acaaccgccg gttgtacgag cgtttcctgg
      661 cagtcaagtt ggttcttgaa ggccacaccc ggaaagaagc cgggcacacc atcggccgcg
      721 acgagcatac tgtcagccac tatgtgaaaa actactgcca taatggaatc caaggtttag
      781 tctccaaaaa acaaagcgga cgaccttcac ggctgacaga tgaacaggaa gcggaactgg
      841 tccaaatcgt cgcctaccac actccggagg aagtaggttt caaatcccgt gccaattgga
      901 cattggcgat tgtgtgtgac ctgattctcc gggaatggag ttatggatat acgcaaaaag
      961 gcgtggcgga cctccttcac cggctaggac taagctggac ccgtccgacg tataccctca
     1021 aaaaagcgga ttccaagaaa caacaggtat ttttggaaga aaccttcccg agcctaaaaa
     1081 aaaattgctg aatagcgata ttgaccacct cctattcgag gacgaaacca tgatccggga
     1141 ttaccaagcc attggctgca cctggtttct ccgcggccac cagcgaaaaa tccccactta
     1201 tggacaacac aaaggggtaa aattgatagg caccttggat tatgaaaccg gtcggatcat
     1261 ttgtacagaa gaaggggcat acgacgcagc ggccttcctc cgttttctgg agcggacgtt
     1321 gcgggaatac cccaccggaa aaatcgtgat ggtattggat aatgccctta tccaccacgc
     1381 caagctgatc cagtcttttc ttgaagaaaa cgctaggcgc ctggcactca tcttcctccc
     1441 tccctacagc cctcagttga acctaatgga aggcgtttgg aagtggctca aggaatcggt
     1501 catcaacaat gttttttttg accacgtcca gaaaatcaaa cagtcggtcc gcggtttctt
     1561 agccgatgta aacgagcgtc cccttgaggt gattgaccga ctgtgcgtgc ggatgtaaag
     1621 tatttaattc aacctatata gttcaggaac aagaagcgag agaagaagag aattagctaa
     1681 aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg gaataggaga
     1741 taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta aagaatttat
     1801 agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga
     1861 cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat
     1921 agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact
     1981 ccagttatat ttccatacca atggaattga tcgtacggat attgctattc caccagaacc
     2041 aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga
     2101 agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca
     2161 caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc
     2221 aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta
     2281 ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata taggagtaac
     2341 ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa
     2401 aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt
     2461 tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac
     2521 tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata
     2581 gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag
     2641 acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg
     2701 cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac
     2761 gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc
     2821 ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttct
     2881 cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat
     2941 tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt
     3001 ggggattgat tgcttttgtt tctatattga atccggagca ggataccata agaacagata
     3061 ctatatattt ccatatccac caaaatagtc tcttagatat ttttaaattt tctccaaata
     3121 ctttcaatta ttgtgaaata tttcccttta attaatcaaa ttttttataa aagtaatata
     3181 taaaataaat tgcttgaagt attatattta gatatcataa tttatggaag aaaaattaat
     3241 taaaaaggat gagattataa tggggttact ggttccgaga gctaatactt atactatacc
     3301 tttaacgaaa cgccaattaa ttgctaaaag atatcaaagg attactagag ccatcaatag
     3361 agagttttgg aattctgaaa gtgatactgc tcacagttta tacgtaggtt cttatgggag
     3421 aggaacggca attagtacta gtgatataga tataatagtt gaattaccga tggcagaatt
     3481 tgacagattc aaaaattatt tatcaaatgg cccgtctaag ttattacaag ttataaaaaa
     3541 cgcatttcag gaaatactac caaatagtga tattagagct gatggacaag ttgttaaaat
     3601 aaatttccat gatggaatca aatttgaaat agttccagct tttaatgaaa aagattattg
     3661 gggtgagtca aaagggttta tttatccaga ttcaaacatg ggagggaact ggaaagctac
     3721 aaatcccaaa aaagagcaag aagccatgaa attaaagaat acaaaaagta ataatttatt
     3781 atatgctacg tgtaaacatt ttcgacatgt acgagataca gaatttacga gttatcatct
     3841 ttcaggaata gtaattgaca gtttcgtata tgaggctatg ggaaattgga aattcgttga
     3901 aaataacagt gggggtcaaa acattagttc tgtatcttat gagacagctt tattagaata
     3961 ctataactca cataaagtta tgggcggttt aaatttatac tctcctggaa gcaatcaatt
     4021 tgttaattct gatagtagta tcatatgttt agaaaaagta ttaaaaaaaa tagctttata
     4081 aagttttatg gaggaacaac atgggagacg aaaaaaagcg cttggaagtt caattacgtg
     4141 aagcatatgg acgagtaacg tatacttaca ccactcattt aaagcaagcc gataatatta
     4201 tttctgacaa taaaatttta aggtgtcttc aaattggttt gtcagccctt actactggag
     4261 gggtacttag taccattatt actgataata gtgagttaac ctttgttgct ggattatgtt
     4321 caacaatttc tcttggatta aacttatact ttaagaattt tgatcttgct gaagaagcta
     4381 aaaatcatca aattgttgcc gataaattat ggttgatcag agaagaatac gtaagcttac
     4441 taacagattt aaatcgttta agtgttgaag atattgtgaa aaaaagagat caattaatga
     4501 acgaaactca tgaagcatat aaaaatgccc ctaaaacaaa tagtaaaagc tatgaatcag
     4561 ctcagaaagc attaaaatct gaagaagagc aattttttac agaagaggaa ttggataaga
     4621 tgttaccaga gcatcttaga aaaaagagtt ctaaatattg aactcttttt ttatgcttaa
     4681 aattctcttg taatttttct attcatctat atttaagaag aaatatgatg atagaaacat
     4741 ctcctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
     4801 catctcacca tgaacgtttc ctaaagccac cgcaagccgt cctagccatt gcctagtccg
     4861 acatcgctag attagtgcat ctcgccataa aagttttcta aagccaccgc aagccgttct
     4921 aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag
     4981 ccaccgcaaa ccgtcctagc catcgcctag tccgattttg cttgatcggt tccatttctg
     5041 tttatttagt gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg
     5101 tctgctttga aggtaaaaag tctttcattc ttcaagactt ttaggctttc aagctacgcc
     5161 tgaaagcatt gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgccc
     5221 attatctttt tcgcctgtca taccttgtca gaagtgttta gagcttatct catctttgga
     5281 ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata atttacttaa
     5341 acacttgtat taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca
     5401 ggcgaaaaag ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg
     5461 atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca
     5521 aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag aattttagaa gttaatcaag
     5581 aaatggtaaa caagttaaca aatatcagtg ggaactatga aaagtacgat ggtactttac
     5641 ttgataacta tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag
     5701 cgttcattct catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta
     5761 tgactgataa ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg
     5821 gaaatagcag tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat
     5881 tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag tgaagataga agtttttata
     5941 gagtcacaag ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta
     6001 gtgaatcttt gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag
     6061 ttttcaaagg tggaattaat caatcagtag cgataccaag agagattttt caactagctt
     6121 tgctaaacaa ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc
     6181 caagtgaaaa tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta
     6241 ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag
     6301 aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca agatgggcga aagaacacaa
     6361 gcgttaatta cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc gattcattat
     6421 cagtgggggt atggtcgata tatgctgatg gacgtattga atcaaggaat ttatttaatc
     6481 aataaagcat acgacttacg agataaatca agagaagaat tagcaaaagg aatctttgat
     6541 agaagtacag gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa
     6601 acagggtttg gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt
     6661 tcagataaca atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt
     6721 ctagatgata aaaccagatt gcgattgttt gattttgagg gtcaacagat aggactaaac
     6781 aagttttgtg aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta
     6841 ttgctagaaa attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa
     6901 agaaaagacc agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg
     6961 attgaattat tccaaaaaag ttatggaacc atagaaacta ccgcaagttg caaatactac
     7021 tttcaaggaa agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag
     7081 aaaatcattg attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca
     7141 acgattaatg gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta
     7201 gaagatgaag gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac
     7261 gagatagaat ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg
     7321 gaaacagaaa atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat
     7381 gatgaaattg aagattggtg tgtgtggtta gtagggacag atgattgcgt tgcatattac
     7441 acaacagcag aataggagtg tgaatcagca tggaaggaat ggattttata gatcatgaag
     7501 atttgattga ttttggttat acatggaaag gaatggtagg tattagcaga agtcttgcta
     7561 atgcctttta cgaaagaaat tatgcagtct atgtccttta tgatgacaat acagaaagcc
     7621 ttgttgatga agagtataaa ttggatttag agaatgtttt atatgggata gaaaaagaag
     7681 atttagcaaa atatattttt tcatggttgg gtcaataaac gaaagagggt agcaacatga
     7741 caaaattcaa aagtaaaggt atgcaatggt gttcttatta ttatgttggg aatgatgaag
     7801 agcttttgag aaaagccaaa caaaaaggtt atccagataa gatgttaaaa cagattggct
     7861 attacatgcc agaagagaga atagaaaaag aatcttgtaa ggttcttttt gatagtgaac
     7921 agctattagt tttgttaact caaaataaat tgatttgtca atttgaaaat aattaatatg
     7981 gggtgaagaa ccatgaatga gaataagatg gtattaagtg aatacgcaag attgagaacc
     8041 attaattggt tgaatggtat gcaaagtagt ggcgcttttg atggatttga tgaggagaat
     8101 atggactttg aaagtttatc gaatgaggat attagatatt attttatcga atggtatttt
     8161 acagaaccag atgaagaggt ggaagatgaa ccagaattaa ttttagaacg tcaaagaatt
     8221 ttattcagtg aacaagatag taagaggttg tatggtttat atgacttaca actgaaagga
     8281 ttaaaagcgc agtatgttgc tgatagttac gatgatatgg tagaacaggg ggcagatagg
     8341 gttgtcactt attcagaaga tgatgaagag gaatgggaaa actattcaga tgatgagata
     8401 tgctttaatg atgggtatat gcctattgaa atcaccgaag aagaatatga taaagtgtcg
     8461 gatagcgatg agattggtat gtgtacggtt gtaagtggat tggatataga tgtccaaaga
     8521 gtagaggttg cataccagaa gatgaaagaa cgtataaaaa gtaaagtaga atcttgattt
     8581 gattggattg tttgcaaagt agctaattaa gagtataata taatcataaa tgtagttaat
     8641 ggatataata atgagtatat tatactcaaa agaggtgaac gaaatgaagg taaaaaatcc
     8701 aaaagaaaaa agcagaattc aagtcggaat agacaaggat ttaaaagaaa acgcagaaat
     8761 gatattggaa gagctagggt taaatccaac aacagcaatc acaatccttt ataagcaagt
     8821 ggtcgctaga ggtgagtttc cagtagagat aaagctaagc gaagaggaaa agcaaggaat
     8881 cagattgcaa cagctaacta tggatatgcc agttgatgtt ctagatacag atgaaaagtt
     8941 agaagagtgg ttcaatgaag cctaatgaga tagtaacggt ctatgttgca tttacagata
     9001 aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat agagtagaat
     9061 tttttggaat cacaagtcaa tatgagaata aatcagacca aataaagaga gtgtattttc
     9121 ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc atggattgac gtagggtctt
     9181 taaaggctat tcccaaaagc cgagagaaca tatcatttaa aaaggttggt atgctatcag
     9241 ctattgatac gaaaaagcta aataggttta tcgagagatt acaaagaaaa gtctaacaag
     9301 gcttttcttt ttttgtttat atgtatatta agatatacgt ttatataaac gtatttatgt
     9361 atattaaaat acacgtaaat atatacgtga ttataaaaat taagaaaata ttaagtattg
     9421 ttaaatagtg ataattagta atatacgaat tataagatac attttatatt taatgtatgt
     9481 aaataaataa aatgactgac ggatattgaa tagtaataaa taacaataat tcagacggat
     9541 acagtgagtg aagtagcttg ctacgaacga acaggcagaa tgaaacggta gtttcattag
     9601 agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact ggcaagttac atagagagag
     9661 tttgcttgca aacgaacgtt acccacttga atagaaaaca aaaataacat attagtgaat
     9721 tttgaatagg agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa
     9781 gacgaacgaa cggtcgcgta gc
//
LOCUS       DAZKSR010000104         9665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000104 DAZKSR010000000
VERSION     DAZKSR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.105"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG8XG_002472"
     CDS             complement(<1..632)
                     /locus_tag="ACG8XG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0774358.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG8XG_002473"
     CDS             975..1391
                     /locus_tag="ACG8XG_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0774359.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG8XG_002474"
     CDS             1388..1858
                     /locus_tag="ACG8XG_002474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0774360.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG8XG_002475"
     CDS             1872..2645
                     /locus_tag="ACG8XG_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0774361.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG8XG_002476"
     CDS             2659..3471
                     /locus_tag="ACG8XG_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0774362.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG8XG_002477"
     CDS             3525..4490
                     /locus_tag="ACG8XG_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0774363.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG8XG_002478"
     CDS             4606..7329
                     /locus_tag="ACG8XG_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0774364.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG8XG_002479"
     CDS             7342..7974
                     /locus_tag="ACG8XG_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0774365.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="ACG8XG_002480"
     CDS             8873..>9665
                     /locus_tag="ACG8XG_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774366.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3261 a         1477 c         1883 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttaattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAZKSR010000105         9571 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000105 DAZKSR010000000
VERSION     DAZKSR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.106"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..1455
                     /gene="lysA"
                     /locus_tag="ACG8XG_002481"
     CDS             196..1455
                     /gene="lysA"
                     /locus_tag="ACG8XG_002481"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0774367.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1442..2242
                     /gene="dapF"
                     /locus_tag="ACG8XG_002482"
     CDS             1442..2242
                     /gene="dapF"
                     /locus_tag="ACG8XG_002482"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0774368.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2245..3126
                     /gene="dapA"
                     /locus_tag="ACG8XG_002483"
     CDS             2245..3126
                     /gene="dapA"
                     /locus_tag="ACG8XG_002483"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0774369.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3123..3839
                     /gene="dapB"
                     /locus_tag="ACG8XG_002484"
     CDS             3123..3839
                     /gene="dapB"
                     /locus_tag="ACG8XG_002484"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0774370.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3845..4540
                     /gene="dapD"
                     /locus_tag="ACG8XG_002485"
     CDS             3845..4540
                     /gene="dapD"
                     /locus_tag="ACG8XG_002485"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0774371.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4556..5557
                     /locus_tag="ACG8XG_002486"
     CDS             4556..5557
                     /locus_tag="ACG8XG_002486"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0774372.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5554..6915
                     /locus_tag="ACG8XG_002487"
     CDS             5554..6915
                     /locus_tag="ACG8XG_002487"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0774373.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6875..8026
                     /locus_tag="ACG8XG_002488"
     CDS             6875..8026
                     /locus_tag="ACG8XG_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0774374.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8001..9170
                     /locus_tag="ACG8XG_002489"
     CDS             8001..9170
                     /locus_tag="ACG8XG_002489"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0774375.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9345..>9571
                     /locus_tag="ACG8XG_002490"
     CDS             9345..>9571
                     /locus_tag="ACG8XG_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0774376.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         3145 a         1662 c         2148 g         2616 t
ORIGIN      
        1 tttatgtata aatagcttct aatagttcgg gtattttgtt tttataacca tttttattct
       61 tatttttaaa aaaatatttc gaaatatact agacaattca gaaaattttg tttaatatta
      121 ttccaataaa attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa
      181 aagaggaatg aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca
      241 gaaaagtata agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg
      301 tcattcaagc gaggctttgt atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa
      361 gcattccaaa cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt
      421 gtcagtggtg gggaaatcta taccgctttg caagctgatt ttcctagaga tactatctac
      481 ttccatggta acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg
      541 catattgttg ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat
      601 ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa
      661 tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc
      721 caacaagccc ttcagttgat aaaagaaagc agctatctag agttagaagg atttcatgct
      781 catatcgggt cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg
      841 aattacttaa ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt
      901 cgttatacac aaaatgatca gccgatccca gtcgaagagg cactaaaaaa actgattgaa
      961 tatacggaag aagctttgga aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg
     1021 ggtagaagta ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag
     1081 acaccacata aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct
     1141 ctgtatcagg cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag
     1201 aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat
     1261 ttgccgaaag cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac
     1321 tcgatgagca gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc
     1381 caatcaagac tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc
     1441 tatgatcctt cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga
     1501 agacacagat tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatataga
     1561 cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag
     1621 ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa
     1681 catgatgaat gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt
     1741 gttggatccc tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga
     1801 aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga
     1861 aaaagtaacg atcactagtt tatttttagg aacgattcat accgttgttt ttgttgaaaa
     1921 tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa
     1981 agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac
     2041 ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt
     2101 ggttgctaag gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg
     2161 agacttgatg atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg
     2221 gacaagaata ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg
     2281 atgaatgaag agggatcagt agattatgct ggtttggaaa aattgatcca attccattta
     2341 gatgaacaaa cagatggatt attagtttta ggtacaacag gagaatcttc tacgctcaca
     2401 caatctgagg aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg
     2461 gtgattgcag gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt
     2521 gcttctttgg gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat
     2581 gcaggactag ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg
     2641 tataatgttc cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca
     2701 gaacatccta atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat
     2761 gccgctcggt tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta
     2821 ccagtaatga gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa
     2881 atagtccatg aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag
     2941 caattaaatg cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc
     3001 aaagcagcca tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct
     3061 ctttcaaaag agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa
     3121 aaatgaaaat cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac
     3181 aagaaagagg agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg
     3241 cttttacgga actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa
     3301 tcctttcgta tagcttatca tatcaacttc cgttagtcat tggcacgaca ggttatacac
     3361 aatcacagtt ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta
     3421 atttttcgtt cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc
     3481 aaggctggca gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg
     3541 gtactgcaaa tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca
     3601 actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt
     3661 ctttacctgg tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac
     3721 atgaatcttt ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga
     3781 aagatcagtc accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa
     3841 aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt
     3901 gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga
     3961 aggaagcagc ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga
     4021 atcagaaaaa atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt
     4081 gctagatatt aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt
     4141 gtcaatcggc aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat
     4201 cggtgaaaac acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa
     4261 aaactgccat attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa
     4321 accggttatc gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt
     4381 ccatatcgga aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc
     4441 agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa
     4501 agaaaataca gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg
     4561 cgaaatagat acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt
     4621 gttagaagaa tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc
     4681 cggtaaagtt tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc
     4741 atttgaagga tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc
     4801 gccaatcgca cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga
     4861 ttcggatatt ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct
     4921 gattgcaaat ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga
     4981 agcctttggt atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca
     5041 aaaaggcatt gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta
     5101 taatattagc caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa
     5161 agaagaacag aaaatgataa atgagacccg taagattctg catcagcctg ctttgccagt
     5221 atcagcaacc tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca
     5281 actgcaaaaa gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat
     5341 tcaattagaa gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac
     5401 agatatggtt tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt
     5461 ctatacgact gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc
     5521 agctttaatg cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa
     5581 ttcggcggaa catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt
     5641 tcagatccca atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat
     5701 gacaaagtaa cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg
     5761 acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg
     5821 cagttaccta ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata
     5881 actgaagatt atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat
     5941 cttggttacc agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc
     6001 gatcaggaaa aaacagcaca ggaaatcaca aaagcattta aaagatatcc gaaaatggtt
     6061 gttccagggt tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga
     6121 agcgacatta ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg
     6181 acggatgttt caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc
     6241 gaggaaatct cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat
     6301 gaagcagctg ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat
     6361 gatcctttcg ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga aaatggactg
     6421 acaggaatag ctggacgcaa ggattttctt tccatcactt tattcaaacg tcatatgtcc
     6481 gatgaaatcg ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc
     6541 gaacatattc cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc
     6601 gataagttat ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc
     6661 gtgatcgaag acttagcttt gatctctgtc gttgggggtc cgcataaaga attgattgga
     6721 ttatctggga aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca
     6781 caaggagcac aggagttgaa tctgatcatc ggtgtaccga ataatcaata tgaaacagta
     6841 gtgaagggta tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact
     6901 ccatcaaatt cctgaaatcg ggtttcatga attcaagaca aaaaaatatt tatatgaaca
     6961 agtaaaaaac agcgaggccg ttatccatga aatcggcgaa acaggactac tcctttattt
     7021 tgataatcag caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa
     7081 agaagaaacg aatctctctt ttgcttctaa gcatgaaggt tatatgcatg catgtggtca
     7141 tgacggacat atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga
     7201 tcttatttat aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca
     7261 aagcgttatt cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca
     7321 tctatggcct ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca
     7381 aagcagtgaa acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa
     7441 aggaatcgat agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg
     7501 gcaattaccg gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat
     7561 ccgaaatgtt ttagctggag aagttattgt atccggaagt atccgttctt acagttcaga
     7621 tatgcaaaat tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc
     7681 tactatttct tttcgctata atgacggcta tccagctgta atcaaccata gaggcttata
     7741 tgcagcactg ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga
     7801 agattttggc atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga
     7861 tacccctgct ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt
     7921 agaatggtac aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagttc
     7981 aaaagaggag cgagtaaata atgatgaatt tatccaatcg atttagtcct aggctttcta
     8041 gaatagaagt atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga
     8101 tcaaactcac cctgggagaa ccggattttc caactcctga acatgtcaag caagcaggaa
     8161 tagcagcgat tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc
     8221 gggaagcggc ttgtatattt cagcaacagc gttatggatt aacttatgat ccgcaaacgg
     8281 aagttttgac gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg
     8341 aagaaggaga caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg
     8401 aactagctgg tgctgaattg atcccgatag atacttctga cacagggttt gtctgtcagc
     8461 cggaacaatt tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa
     8521 actatcctaa caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg
     8581 aggttttaaa aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt
     8641 atagtggaac gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg
     8701 ggttatccaa gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag
     8761 cccttattga cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt
     8821 cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag
     8881 agcaatatca aacacgaaga gactatctgg tccagcaatt ggcgccttta ggatttgaag
     8941 tgacccagcc aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg
     9001 actcttggca gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa
     9061 ttgcatttgg cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa
     9121 aattacatga ggcctgcaaa cggatcaaag actttttaac agaacagtga ttcatagatt
     9181 ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc aaggagacga ttgtttttta
     9241 aatttagttc aaatgaacgc gtttcttttg attcataact aatgtaatgt taagtttaaa
     9301 ttgtttagaa tgattttaaa agaatattca aggaggtttc atcaatggaa aaaatcaaag
     9361 ttgtcattgt aggtgcatct catggagggc atcaatcgat cttggaacta ttgacacgat
     9421 acggtgaaaa tgtagatatc acgctatttg aagcaggaga ctatatttcc ttcatgtcat
     9481 gtggtatgga gctgtattta gaagacaagg taacagatgt aaatgatgta cgtaatttca
     9541 gaccagaaaa ctttccgcaa tccaatgttc a
//
LOCUS       DAZKSR010000106         9432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000106 DAZKSR010000000
VERSION     DAZKSR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.108"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG8XG_002491"
     CDS             <1..680
                     /locus_tag="ACG8XG_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774377.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG8XG_002492"
     CDS             800..1000
                     /locus_tag="ACG8XG_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0774378.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG8XG_002493"
     CDS             1076..1273
                     /locus_tag="ACG8XG_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774379.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG8XG_002494"
     CDS             complement(1570..3117)
                     /locus_tag="ACG8XG_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0774380.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG8XG_002495"
     CDS             complement(3201..4004)
                     /locus_tag="ACG8XG_002495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0774381.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8XG_002496"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8XG_002496"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0774382.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8XG_002497"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8XG_002497"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0774383.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8XG_002498"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8XG_002498"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774384.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG8XG_002499"
     CDS             complement(6389..7318)
                     /locus_tag="ACG8XG_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0774385.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACG8XG_002500"
     CDS             7513..8196
                     /locus_tag="ACG8XG_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0774386.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACG8XG_002501"
     CDS             8357..9400
                     /locus_tag="ACG8XG_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0774387.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAZKSR010000107         9235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000107 DAZKSR010000000
VERSION     DAZKSR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.109"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG8XG_002502"
     CDS             complement(137..427)
                     /locus_tag="ACG8XG_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774388.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG8XG_002503"
     CDS             complement(545..805)
                     /locus_tag="ACG8XG_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774389.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG8XG_002504"
     CDS             complement(942..1355)
                     /locus_tag="ACG8XG_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0774390.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG8XG_002505"
     CDS             complement(1811..2296)
                     /locus_tag="ACG8XG_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774391.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG8XG_002506"
     CDS             complement(2435..2650)
                     /locus_tag="ACG8XG_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0774392.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG8XG_002507"
     CDS             2874..3674
                     /locus_tag="ACG8XG_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0774393.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG8XG_002508"
     CDS             3693..5561
                     /locus_tag="ACG8XG_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0774394.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG8XG_002509"
     CDS             5554..6045
                     /locus_tag="ACG8XG_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0774395.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG8XG_002510"
     CDS             6033..6587
                     /locus_tag="ACG8XG_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0774396.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="ACG8XG_002511"
     CDS             6605..7600
                     /locus_tag="ACG8XG_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0774397.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7742..8992)
                     /locus_tag="ACG8XG_002512"
     CDS             complement(7742..8992)
                     /locus_tag="ACG8XG_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0774398.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3190 a         1498 c         1768 g         2779 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga gtttagtcta atggtgataa
     7621 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     7681 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     7741 gttatttctc tatcacactc cttggtggcg tatagagttg gtgtttacgt agtagcgtat
     7801 ccaccagacg cgtaaatttt cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg
     7861 gaacctcctt atatttactt tgataaaaag ctttgtattc aggaagataa ttttttacag
     7921 agttggcagc ttcaactaag tagtatctaa aatatcgatt tccttgtttt gtcagaggtg
     7981 tattttgcga ctgatagttt ccagattggt tcactttcca acttaatcca gcgtattttg
     8041 ccaattttgt ctgatcttca aatctttcga tttgaccaat ttcagcgatt aaaccggcag
     8101 catagacttt accaacgcct ggaatactgg ttaagcattg atattctgga atgacaacaa
     8161 ttacttgttc gatggcttta tctaattctt tgatattttt ttcaagtgct cgtatttctc
     8221 gtacgagaac acttaaaaca acattaattg attcttgtgc aagagcacct aggcggtaac
     8281 tcatggtaat tgcacgttga attgctttag cgattttttc tggttgcttg aaacggcctt
     8341 ttcctttttc ttgaagtaaa tcacagaaag cttctaaagg aagctcagca agttcatcca
     8401 atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc gtcgattcgt
     8461 ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga aaatgttgct
     8521 ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc tgtaaggcca
     8581 tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag aaatcagcga
     8641 ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta aattgtttca
     8701 cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc tcatcttcat
     8761 gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca atcacaattt
     8821 gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat gaagcacctt
     8881 cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg tctgttaaaa
     8941 aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc atggaagaag
     9001 gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt cccagaaact
     9061 cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta gaagctgcct
     9121 tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt tggtagtgat
     9181 aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag gaaat
//
LOCUS       DAZKSR010000108         9207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000108 DAZKSR010000000
VERSION     DAZKSR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.110"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..139
                     /locus_tag="ACG8XG_002513"
     tRNA            68..139
                     /locus_tag="ACG8XG_002513"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Glu,seq:ttc)
     regulatory      328..407
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            405..1598
                     /gene="metK"
                     /locus_tag="ACG8XG_002514"
     CDS             405..1598
                     /gene="metK"
                     /locus_tag="ACG8XG_002514"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0774399.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1728..3200
                     /locus_tag="ACG8XG_002515"
     CDS             1728..3200
                     /locus_tag="ACG8XG_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0774400.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3283..4071
                     /locus_tag="ACG8XG_002516"
     CDS             3283..4071
                     /locus_tag="ACG8XG_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0774401.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4138..4806)
                     /locus_tag="ACG8XG_002517"
     CDS             complement(4138..4806)
                     /locus_tag="ACG8XG_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0774402.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4921..6633
                     /locus_tag="ACG8XG_002518"
     CDS             4921..6633
                     /locus_tag="ACG8XG_002518"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774403.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6636..8408
                     /locus_tag="ACG8XG_002519"
     CDS             6636..8408
                     /locus_tag="ACG8XG_002519"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774404.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8490..>9207)
                     /locus_tag="ACG8XG_002520"
     CDS             complement(8490..>9207)
                     /locus_tag="ACG8XG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774405.1"
                     /translation="TFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYL
                     SKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYD
                     YELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQ
                     AYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIY
                     THTTKNMQNQLVEKLGKIEI"
BASE COUNT         2799 a         1523 c         1972 g         2913 t
ORIGIN      
        1 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattta
       61 ttttattggc ccgttggtca agcggttaag acaccgccct ttcacggcgg taacacgggt
      121 tcgaatcccg tacgggtcat ggaagatact ttttgagtat ctttttcttt ttatctgaaa
      181 gaatgatttt aaaaagctta gcagagttat ctttatggat gattgtgcta agcttttttg
      241 gacagttcta atgtaaaata tttttatatt ttttaagcaa aaaaatgaaa aaatacgagc
      301 agaaaactgt tttatctgct atacttggtt cgttgcaagt tcccgaaagg atccgcagga
      361 cggagatgcc ttgtaaccga atttaactag ggggaatcat tataatggta gaaagacact
      421 tatttacatc agaatctgtt tctgaaggac atccagataa aattgctgac caaatcagtg
      481 atgcaatctt ggatgcaatt ttaaaacaag atccaacagc acgagtagcg tgtgaaacgt
      541 ctgtaacaac tggtcttgtt ttagtatttg gagaaatatc tacgacggca tatgtagata
      601 ttcaaaaagt cgtacgggaa acaataaaag aaattggtta tacacgtgca aaattcggat
      661 ttgatggaga tacagcagct gtattggttg cgattgatga acaatctcct gatattgctc
      721 aaggagttga tgaagcactt gagatccggg atgaagataa aaaagacgta ttagatgaga
      781 ttggtgctgg tgaccaaggt ttaatgtttg ggtttgctgt agatgaaaca ccagaattga
      841 tgcctttacc aatcgctttg agtcatcgtc tggtacgacg cttggcagat ttgcgtaaat
      901 caaatgaatt aacgtattta cgtccagatg caaaatctca agtaacggtt gaatatgatg
      961 atcaaggaca accggaacgc gtagatacaa tcgttatttc aacacagcat gatgatgcag
     1021 tagataatga aacaattcgt catgatgtca tcgaaaaagt agtgaaggaa gttattccag
     1081 ctgaattatt agatgatcaa acgaaatatt atatcaatcc gactggccga tttgtcattg
     1141 gtggtcctca aggggatgcc ggattaacag gaagaaaaat cattgttgat acgtatggcg
     1201 gttatgctcg tcatggtggc ggtgcttttt ctggtaaaga tgcgacaaaa gttgaccgtt
     1261 ctgccagcta tgctgcacgt tatattgcta aaaacattgt tgcagcaggg cttgctcgca
     1321 aagcagaagt acagctagct tatgcgattg gtgttgctca acctgtttcg atctcaatca
     1381 atactttcgg tactggaacg gttccggaag aagaactgat tgctgcagtg agagaaaact
     1441 tcgatcttcg gccagcagga atcatcgaga tgcttgattt gcgtcgtccg atttacaaac
     1501 aaacagctgc ttacggtcat tttggtcgta cagatgtaga tttgccttgg gaacatacgg
     1561 acaaagtaga tgcgttgaaa gcaagtttag caaaataagt tatattcagc tggaacgatc
     1621 gccaagtggt ggttgttcca gcttttttta tggcagttaa cctcttgaaa aagatagtaa
     1681 ttatttatct atttttagat cctgtcataa tgaaagggag gaataagatg aaaaaagaaa
     1741 caaatgttac tttggtcaca gtgagtattt tcattgcaac gttcatgacg gctattgaag
     1801 gtacaatcgt aacaaccgct atgccaacga ttgtcggttc attacatgga atggaaatca
     1861 tgaactgggt attttcgatt tatttgctga caaacgctat gcttactccg atttacggaa
     1921 aacttgcgga taaagtggga cggaaaccaa tctttatttt tgggacatta ttgtttatta
     1981 tcggttcgtt attttgcggt ttatctcaaa gcatgattgg gttgattgct gctcgtgcga
     2041 ttcaagggat gggtgctggg gcaatgatgc cagtagcttt gacgatcatc ggcgatcttt
     2101 attctattga aaagcgagca aaagttttag gtttgaatag ttcagcctgg gggattgcca
     2161 gtgtttttgg accgcttgcg ggaggattca tcgttgaaac gatcagctgg cactggatct
     2221 tttttatcaa tgtgccgatc ggaattctat taattatttt gatttggcta tatttaaatg
     2281 aaccaaaaat catacatgag tctaaaccaa tggatatttt tggtagtttt agtttgatgg
     2341 ccatgcttgt ctcattgctg ttagggttcc aattattagg agacgaagga atgagtccgc
     2401 ttgtttctgc tttgctgcta ggtagtatcg taatgttagg tatttttgtt tttgccgaaa
     2461 aaagagctaa agatcctgtc atcatgatgc atttattcaa aaatccacta tttgttgtgg
     2521 tcaacttggc tgcagcattg gctagtggtt ttttgatggg aatcgatgtt tatatcccta
     2581 tgtggatgca aggtgtatta gggctgaatg cagcaattgg tgggctggta ttagcaccat
     2641 tatcactagt ctggatggtc ggttctttcc ttgcaagcaa gtggatgact caaatgaaaa
     2701 ctcattgggt cttgagaatt ggactactaa tcgcagctat tgggggcgct tggttgtatc
     2761 ttatgccttt cagaagtaac tatttatggt tttttgctat atcaacggtc atcggtatag
     2821 gcttaggtgt gaccatgact acctcgactg tcagtgcaca aaatagtgta tctcgtgacc
     2881 agcttggtgt agcaacttct tttaatacgt tagttcgaac aattggtcaa acagtcatgg
     2941 tttctatttt tggtttattg ataaatgggg taactaaacg agaacttgca gcagcaaatt
     3001 taacaaatga tgcagatatc atgaataaac tggtgaatcc acaaacggct aaatatattg
     3061 atgcggatat tttgttacca ttaagaaaaa ttctttatga tgggttacat tccgtttatc
     3121 tagttggctt gttactcgtg atcattgctt tggtcttaac gcttttcgtt catgaaaaaa
     3181 gtggtaaaat aaaacagtag tttcggttaa gatatgtacg tagccgaatc attcaaattt
     3241 aaagttattc aaaaataact tcttactaag gaaggcgaaa atatggcatt tattcaagca
     3301 aatatttatt cgaatgtgtt agaaatggaa gtcaatctaa atgtgatact accgcaaaca
     3361 acaaaaaagg tgattggcac cagttcagag caagagacaa cggatatacc tgttttatat
     3421 ttacttcatg gaatgggtgg caatcatagt gtatgggaaa gacgtacgtc gattgaacgt
     3481 tatgtagcaa actacggatt agctgtcatc atgccatcta ctgatcttgg ctggtacaca
     3541 gatacagttt atgacatgaa gtattggaca ttcattgcag aagagctacc tgagatttgt
     3601 catgaattat tcccgcaatt aacaagaaaa agagaaaaaa catatgccgc tggattatcg
     3661 atgggcggat atggcgcgtt aaaactaggc cttgcaaaat cagaaaattt cgcagcagta
     3721 atctctcttt caggcgctgt ttctgtagga aatcgaatgg atgatctttt gatggttcgt
     3781 aaagcatctt tttgggaagg gatctttgga ccattggatc aaatcgaagg ttcaaaaaat
     3841 gatccgcaat acttattgaa agaactagta aaaagcggaa aaccagttcc aagattttat
     3901 atggcttgtg gggaatctga ttttctttat caggcaaatc aggaaatggt atctgctatg
     3961 caggaacaag gcttggcggt cacctttgaa caaggacctg gcgatcataa ctggatcttc
     4021 tgggaccaat ggattcaacg tgcattggcg tggctaatgg aagaaaaata aaaaaagagt
     4081 cttaaaaaga tagctcctat agagatcaca aacatagtga tctctatagg agctttttta
     4141 ctgctcgttt tctcttcttt tattttgttg gcataagcct tcaatatagg tttgtgcact
     4201 cgttagaaag agtgcttttt cattcaattc tgcatgggac tgcatacttt ctttaaatcc
     4261 ctctaattga ttttcaacta cataattacg cataaataga actgtttctt tcaaaaattc
     4321 atttccgtcc ataatttttt gtcgaagctg ctgttcatgc tgctgatcca ttaactgtcc
     4381 aatcaataaa ttgtggagag aggcaatggc ctgatgactt tgggtgactg aaaatccttc
     4441 acgaacaagt aattggatca tttgctccaa atgttctaat cgactcggaa aagcgggcac
     4501 agtattgatt tctaattctg ccccacaagg gaaacgtgta taaaggtcat agtaattttc
     4561 catatactgc agcaactgtt cattccaagg caaagttgga tctggaagcg ctaattcttc
     4621 ttccattgtt tcagccattt tttgcaagag ctgttgtttg tttttgaaat accaatataa
     4681 agcaggtgct tgaacatgaa ttctgtttgc gatgtttcgc atggaaagct gcgaaagagc
     4741 ttgtgtttca gccaggatat caaaggcggc ctgaatgatg attgcttgag aaagctttat
     4801 ttccatttta tcaacctcgt gtattgcatt atctttttaa acatggtaaa ttgttagtcg
     4861 gtctaatttt acagtgttaa attagtcctg tcaatttaaa caaaggggtg taatcaacga
     4921 atgaaattaa tcttacgaca tgcgaaaaaa tataaagtat ctgtttttat ttccttgctg
     4981 tccgtagcag tcatggttgc agcagcatta tggcaaccta agttactaca gcaagtattg
     5041 gaagctatta ttacagaaga taatgatgaa atgaaaacaa ttggtattta cctgatcagt
     5101 ctggctttgc taggattagc cgcaggtgta acgaacacga ttttctctgc aaaagtagct
     5161 caaggcgtaa gtgcagatat ccgagaagaa gcatttcgaa agatccagac attttcattt
     5221 ggaaatatcg aacagttctc tgctggaaat cttgttgttc gtctaacaaa tgatatcacg
     5281 caaatccaaa atttagtgat gatttctttg caatcacttt ttcgaattcc attcttattt
     5341 attggtgcct tcattttagc tatgatcacg atgccgcaat tatggtggat catcgtagcg
     5401 cttatcgtta cggtatttct gattacggca ctttcattta caaggatggg caagcacttc
     5461 atgatcattc aaaaactgat tgatcgtgtg aatagcattg caaaagaaaa cttactaggg
     5521 attcgtgtag tgaaatcttt tgtacaagaa gaaaaccaat tgaagtcatt cagcaaagta
     5581 agtgaggact taacaaaaca caacatcata gtcggtacat tattttctgt gatgatcccg
     5641 tccttcatgc tggctgccaa tttggctgtg gttggggcga tcttttttgt gagcgatctt
     5701 gttaaagatg atcctacgtt aattggtgga attgcttcat tcatgaacta cttgatgcag
     5761 attatgatgg caatcattat tggcggaatg atgatgatga tgacttcaag agcagccgta
     5821 tcgctgaagc ggattgcgga aattttagat gctgaaccag atcttactta tttagacgtt
     5881 ccagaacaag agttgacggg ttctgtcact tttgaccatg tttcttttcg ttatcctggt
     5941 gaggatacag atacgcttaa agatatcagc ttttctatca aacctggaga aatggttggt
     6001 atcgtcgggg cgactggtgc cgggaagtca acactagcac aattgatccc tcgtctattt
     6061 gatccgacag aaggcaaagt tgaagtaggt ggtgtagatc ttcgtcaagt aaatgaaaag
     6121 agtttaagac aaacggtttc ctttgttttg caaaaagcaa ttttattctc aggaacgatt
     6181 gctcaaaact tgcgccaagg gaaaaagaat gcaacagaaa aagagatgga acatgcttct
     6241 tctattgctc aagcaaaaga attcatcgaa aagttatctg atcgttacga agcaccaata
     6301 gaagaacgta gcagtaactt ttctggtggt caaaagcaac gtttatccat tactcgtggc
     6361 gttatcggta atccaaagat cttgatccta gatgacagta cgagtgcact ggatgctcgc
     6421 tctgaaaagc tggtccgaga agcactcgat cgagatttga agggaacaac gacgattgtc
     6481 atcgcacaaa aaattgcttc tgttgtcaaa gcagaccgca tcttagtatt ggatgaaggc
     6541 agattagtcg gcgaaggaac ccatgaggaa ttggtagctg gaaaccgtgt ctatcaggaa
     6601 atctttgaaa ctcaaaaagg aacggaggag taagtatgac tgatctaata aaagcaagta
     6661 aatttttcta tcactatttg aagcggtaca aactctcctt tttctttatc tttatcacag
     6721 tgatcattgc tacctatcta caagtaaagg cacctcaata catcggggaa gcttttcaag
     6781 aattagctaa ttacgtagga ggcttgatgc aaggcgtaga cgataaaagc aaatttgttt
     6841 ctgtaatatg gaaactcttg ctgttttatg tactagctag tgcagccaac tttatctata
     6901 gtattttgtt cacacaagta gtagggaagt caacgaaccg aatgcgtatc ggtctattca
     6961 acaaattgga aaaattgacg atccgtttct ttgattctca tcaagacggt gaaattttaa
     7021 gtcgttttac cagtgactta gataatattc aaaatagttt gaaccaagct ttgcttcaag
     7081 tcatcacgaa tgctgtttta cttgtcggga tcttgatcat gatgttccgc caaaatgtag
     7141 agttagcttg ggctacaatt gcttccactc cagtagccgt tttgatagct gtcttgatta
     7201 tccgcaaagc acggaaatat gtggatcttc agcaagatga agtggggaaa ctaaatggtt
     7261 atatggatga aaaaatcagc ggacaacgtg tcatcatcac aaatggctta caagaagaaa
     7321 cgattgaagg attcttagag cataatcaaa gtgttcgcga agcaaccttc aaaggacaag
     7381 cttattctgg tctactattt ccgatgatgc aaggaatgtc actggtcaat acagcgatag
     7441 ttatcttttt cggcggctgg ttagcattaa atggtgattt ggaacgttcg gtggcattag
     7501 gcttagttgt gacattcgtc cagtattcac aacaatatta ccaaccactg atgcagattt
     7561 catctggata cagcatgatc caattagcga ttacaggggc aagacgattg aatgaaatat
     7621 ttgatgaaaa agatgaagtc aaaccagaaa acggtaaagc atttgatggt gttgaaaaag
     7681 gtcttgcgtt gcagcacgta gattttggtt attctccaga aaaactagtc ttgaaagatg
     7741 tctcgatcaa tgtggacaaa ggagaaatgg ttgctcttgt tggtccaaca ggatcgggga
     7801 aaacgacgat catgaatctg ctaaaccgtt tttacgatgt caatgatggt tcagtagtca
     7861 tcgatggaat agatatccga gattatgatc ttgatgcatt acgtagacat gtaggaatcg
     7921 ttttgcaaga atctgtattg ttctccggaa cgatcaaaga caatatcaca ttcggcaaac
     7981 cggacgcttc agatgaagaa gtcatcaatg cagcaaaaca agcgaatatt catgacttca
     8041 tcatgacgtt agaaaaaggt tatgatacag agatatccga agaaaataac ttattcagta
     8101 caggtcaaaa gcagttgatc agtattgctc gaacgatcat cacaaatcca tctttattga
     8161 ttttggatga agcaacaagt aatgtagaca cggtaacaga agcaaggatc caaaaagcaa
     8221 tggatgaagc aatcaaagga agaaccagct ttgtgattgc tcaccggctg aaaacaatct
     8281 tgaatgcaga tagaatcgtt gtcttgcgtg atggggaagt cattgaagaa ggcagtcatc
     8341 atgagttatt agcagaaaat ggattttatg cggaacttta tcataatcaa tttgtatttg
     8401 aaagttaaat taaaagaacc tcgttataac ggggttcttt ttatatattt tatgttgttt
     8461 gtttttataa ggggcagaaa aggggcaatt taaatttcta tttttcctag tttttctact
     8521 agttgatttt gcatattttt agttgtatga gtataaatct ttaatgttgt ttttgaatct
     8581 tcatgtccta cacgatccat aatggctttt aatgggattc ctaattctgt aagtagagaa
     8641 atgtgggaat gccttaacat gtgagaagaa acttttttat ctaggtttaa ttctttggat
     8701 actgctctta aagattgatt atatgcttga atagatatcg ggttaccatt tttagagatg
     8761 aaaatgtatt tatctttatt attcttaatt aatttgtttt cttcaattaa ttgatctatg
     8821 atttctttag cattacttga taattcaatg gttctataag aacctgtatt tttagttgtt
     8881 gttttaattt ttaactcgta atcataagta ccagaaaccg taattttatt accatcataa
     8941 tcagattcag tcaaagatat caattcacca taacgtagtc cagttaaaaa caaaaattta
     9001 gtaattaaag aataacgtga ctgttgatgt gaactttttt gtttactagc aatcatttga
     9061 acttcttctt ttgataaata accatctttt tcttttttta tttcttcgtt tgtcttaggt
     9121 tttcttgcta aagaaacccc atttacggga ttatatttta atccatatcg acggttggcg
     9181 aatttaaaaa tcattcctgt aaaagtt
//
LOCUS       DAZKSR010000109         8985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000109 DAZKSR010000000
VERSION     DAZKSR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.111"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..1407
                     /locus_tag="ACG8XG_002521"
     CDS             172..1407
                     /locus_tag="ACG8XG_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774406.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1491..2579
                     /locus_tag="ACG8XG_002522"
     CDS             1491..2579
                     /locus_tag="ACG8XG_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFQ0774407.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2576..3997
                     /locus_tag="ACG8XG_002523"
     CDS             2576..3997
                     /locus_tag="ACG8XG_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0774408.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            4033..4749
                     /locus_tag="ACG8XG_002524"
     CDS             4033..4749
                     /locus_tag="ACG8XG_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0774409.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            4768..6351
                     /locus_tag="ACG8XG_002525"
     CDS             4768..6351
                     /locus_tag="ACG8XG_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFQ0774410.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            6480..7064
                     /locus_tag="ACG8XG_002526"
                     /pseudo
     CDS             6480..7064
                     /locus_tag="ACG8XG_002526"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            7010..7528
                     /locus_tag="ACG8XG_002527"
     CDS             7010..7528
                     /locus_tag="ACG8XG_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774411.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            7551..8576
                     /locus_tag="ACG8XG_002528"
     CDS             7551..8576
                     /locus_tag="ACG8XG_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0774412.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            8825..>8985
                     /locus_tag="ACG8XG_002529"
     CDS             8825..>8985
                     /locus_tag="ACG8XG_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0774413.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         3254 a         1004 c         1670 g         3057 t
ORIGIN      
        1 ttatttgatg actggaaata gaaattaaat atattgtatt gttacattaa agaaaacttg
       61 tatggaaaaa tcagtacaat accattattt aatgttcttc acttaaagcg caaaaaatat
      121 cagtttatta aactcgtata tagaacaaat atatgtagac ggggcgaaac gatgagtgta
      181 aaaaaaatgc tatttatggt aagtacaata attgtattaa catcccgatt ttggggaatt
      241 aatactagtt tatctatgcg atattttatt atggcaatat gggatttata ttttatgtta
      301 agctattttt cttatcgtga gaaaacatat aaaaaatacg ttatcaagaa taacttttgg
      361 ttgactataa tgccgtttgt gatattttct atctatactc taataatatg gggaattggt
      421 tcaaatgcag aatttgaaaa ttttataaag ttatgttcca cgttgcttta tttaattctt
      481 gcatatggtt acgcatgtac agcattttac ttatttaagg cagaaggaat tgattttatt
      541 ttttggtcag gtgttatttc atatatgagt ggttcagttt tttatttaat tgtatcttat
      601 ggtttaaatg gtttaatttc ttatataaaa agtcttataa caggtgaaac aaatactgca
      661 aattttgcta tggaggtaca tgatttaact tttgcgatgg gggttttttt cctttattac
      721 gtattttttg aaaataaaaa tacaaaaaaa cataagataa aaattatcgt atctttaatt
      781 ttaattttca tgggattgaa gagaattgaa ctgttagctt taagtggtgc aattctagtg
      841 tattatgttt tattaaagtg gggaaaaaag attcaaatcc gtgcattagt gtgtactatt
      901 tgtgcaactg ttatttcgtt agtgtttatt tacattatag atacaggaat tttggcattg
      961 cttatgtcaa atttgggcat tacagatgac ggtgcaaggc tttcatttta tctatattca
     1021 tctaaatttt atgaacttgg tttaggatat gtaggaacag gatggacatg gttttcaaaa
     1081 tactttttta atctttattt atcgcacttt aggttaaatg gtggaagaat tgcagcaagc
     1141 ctacacagtg atatattaac tatttatata gaagttggat gtatagtttt tattatatgg
     1201 atgttttggt tattttttgt gaaaccgata agactaacta aaaaatttgg attgaaaaca
     1261 ggggagtgtg tattattatt aactatatat atgtttatat tatatttgac ggataatacg
     1321 ctcacttatc cagacactca aatgattttt ctcttagtac ctatgatagt ttcgtgggtt
     1381 gataatcaac aacgaatatc ggaatagata gctaaagtat attttaatta tgaatttata
     1441 attagttgta aaagttaata tttaacggac aggaagtagg gtgaaaaaat ttgaaaaaaa
     1501 ttttagtgtt agcaaataca tattatcaaa taatagtagc aatacaaatg aaaaatacaa
     1561 ttttttccga tgagtatgtt gaaattatta tttctgatca ttctaaaaac actaaagaaa
     1621 ttgcgaaaaa aatagaagaa aaaaaagtgt ttgatggctg tcattattta gaaagcaaat
     1681 cttttgatga taatattggg tattttcaaa agattaagga ctgtcttcag atttcttgtg
     1741 gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa aagtgaatat tttgatgaat
     1801 ttatttgttt ttcaatggga gaggtttggt tggaaggaat atatgcttgc ttatatcgaa
     1861 ataataaaaa tgtacaggta tcaattttag aagaaggtat tttaagctat ggagttgaca
     1921 ttattccaac agcaagaaca aaagctatcg cagtactcaa ttacttaaaa aaaaggactc
     1981 cactatataa caaaagctat aatttttatt gtttttttcc taatatatat acaggaagag
     2041 agaatacaat acaaattcca ttagttacag ctgggtcaag aaccgcattg caactttcgg
     2101 atatttttaa ccctatgata gatatttata aagaaaaaaa atatatattt tttacgtctg
     2161 tttatgattt tgagggcgga gaacctgtcg gagaatatga attagtatgc aaaatagcag
     2221 acttagtagg aaaagataat ctaattgtta agacgcatcc tagagatgta agaacaattt
     2281 ataaagataa tggatttatc gaggataaaa attcatcaat accttgggag gcgattcaat
     2341 tgtcaggcga ttttaacgat aaaatattta tgacaattaa ttcaggaagt gttatttcag
     2401 gtagtacaat gtctgcaaaa cctgtacgca cgtattatat gtataaattg tgtaacatag
     2461 aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga gcgattgctt aagcaaaaaa
     2521 cgatgaaaaa cattttaaag aatgtgaaaa tagcagaaag cttagatgat atactatgag
     2581 caaaactaaa tatttaatta aaaatatggg aatattaaca ataagtaatt tttcttcaaa
     2641 aattttagtt tttttgttag tccctttata tacaagtgta ttatccacaa cagaatatgg
     2701 cacatatgat ttgacaattt caacagccac attattgttt ccaattttaa ccttaaacat
     2761 tgtagatgct gtgatgcgct ttttaatgga taaagataaa aataaaaaaa atatagcatc
     2821 tatagcgatt aaagttatat ttttaagcgt gatagtcttt ggatccgtaa tgtatttgtt
     2881 aaatattaca aaattatggc aagatgttta tggattagaa aaatatattt ttttgtatta
     2941 tatatcctat gtatggaatc aatttctaat acaatttgct aaagggttag aaaaagttaa
     3001 agctatgggc atagcaggta ttatcagcac aattgtaaca ataattgcca atattttctt
     3061 cttgcttatt ctgaaatggg gattaattgg ttttttcatt gcaaatattt tatcacagat
     3121 tatttcagct acttatctgt cagtattagt gagaatatgg gaatatatcg attttgaggc
     3181 taaagatcct cagctaaaga gagaaatgct aaattactcg attccattaa ttgcaactgt
     3241 tgttggatgg tggattaata gtacagctga taggtatgtg gtttccttta tagtaggtgt
     3301 ctcggcgaat ggcttattgt ctgtggcata taaaatacct caaataatta atactttgca
     3361 gggaatattt atacaggcct ggcaaatttc tgcaattaaa gaatatggag ataatgagac
     3421 tgcaaaattt tatggaaata catttgttat gattaatttg ttaatgtgca taacctgttc
     3481 aagtcttatc atattaacta gaccgttttc acatattctt tatgcgaaag acttttatga
     3541 agcgtggaaa tatgttccat ttttattaat agctaatgtt ttaaattgtg catcaggtct
     3601 gttaggacct atcttatcag caaaaaaaga ttctaaatcc atgatgtggt ctgcaatcat
     3661 aggggcatta gttaatgtga ttatgagtgt agtccttgtt tataagatag gggttcaagg
     3721 tgccacaata gccactgtta tatgttcata tgtaatatat gttgttcgta aacgagctgt
     3781 gggaaaagat atttttataa agcattatca tattgttgtg gaatcttggg gattattgtt
     3841 tgttcaggca atcatagaaa tatattttaa aaattatgta atagaaatta taattatgat
     3901 tattttaatt attataaata tcagttatat taagcagttg tgttatagag gaagaaacat
     3961 tatttcaatg attaagagtc gagagcacaa acggtagacg atgttatttt tataaaaaga
     4021 tataatttat taatggtcga taattatgaa aggaaaaata ttcttaaaaa aaagagtata
     4081 ggatgtcgta tgaaaatagt cgctatagta ccaatgaagt taaataacaa aagattgcca
     4141 caaaaaaaca cgaaagcttt taccaatgga aaaccattgt gttattatat actttcaaca
     4201 cttttaaaag taaaacagat tgataaagtg tatgtctatt gtagtaaccc agatattaaa
     4261 gaatttatac cagaaggtgt aacattttta aagagatcta gttccttaga tcaggataca
     4321 acgagtatta cacaggtatt agccggtttc acaaaagaag tagaggctga tgtatatgta
     4381 atgacacaca caacagcacc atttattagc gaaaaatcta ttgaaaaagg actagatgcc
     4441 gtgctgacag gagaatatga ttctgcattt gctgcaaaaa aattgcaaga tttcttatgg
     4501 aaagacggtg aaccatttaa ttatgaattg gacaatattc ctagaacaca ggatttgccg
     4561 cctttatacg aagaaactag tggattttat atttataatc gcaatgtgat gaccgagttg
     4621 catagaagaa ttgggaaaaa accttgcata gttgaagtag gtgagattga gtcagttgat
     4681 attgatgaag ccgaggattt tatgattgct gatgcgattt ataatcatct tattttacaa
     4741 ggtagttaag acagggggaa ggttaggatg aacaatgtac agattttaga ttgcacactt
     4801 agagatgggg gctattgtaa caattgggaa tttggatttg ataatacaaa aaaaattatt
     4861 tctggactta tagaatcagg aattgagatt atagaatgtg ggtttctcac aaataaaaca
     4921 gtatacaata aaaatgttac aaaatatacg accttagaag aattagcaca gataattcct
     4981 gaaaaaagag aaggaaaatt atttgtagca atgatgaact atggcgaata taatatagag
     5041 gatcttccag attatgatgg ttcatctatt gatggaatta ggattgcgtt tcataaaaaa
     5101 aattgcaatg aagcgttgaa attatgtcgt gagataaaga aaaaaggcta cttagttttt
     5161 gttcaagcta tggtatctct caattataca gatgaagagt ttttagcagt aataaaaaat
     5221 gtgaatcagt ttaaaccata cgctttttat attgttgata gttttggaat gatgaaagga
     5281 aaagatttaa ctcgtctttt ttacatgatt gagcataatc tagaaaaaga tatacagata
     5341 ggttttcact cacataataa catgcagctt gcatattcta atgcacaaaa acttataacc
     5401 gtacaaacaa atcgaagcct gattattgat tcttcggtgt acggaatggg aagaggagca
     5461 ggtaatctta acacagaact atttgtagaa tatttgaatg ataatgcagg aaaaaaatat
     5521 caattaaaac cattattata tataattgat gaaatactaa atagtttcta tcagaagagt
     5581 tattggggat attcattacc aaactatatt tcagcagttc ataatgctca tcctaattat
     5641 gcaagctatc tgtcatataa gaatacttta acaattgaag caatgaatga aatattcgaa
     5701 atgatggatg aagaaaaaaa ggtatcttat gataaacaat atatagaaga tttatactac
     5761 aaatatatgg aaactgggaa aatacaggag gaatatatgg aagaactgag aaaatttctc
     5821 tcagacaaaa aaattctact gattgcacca ggaaaaagtt ctgtagatga ggtagaaaga
     5881 ataaaagagt ttgttaccaa agaagaagtc gtaataatta gtgtaaattt tgaatattct
     5941 catatagata tagattacgt atttttaagt aatttaagac gttttcatga attaccgaaa
     6001 gaaagcagaa ctaaatgtat tgttacatca aatatacaag catcggatgt atatttacgg
     6061 acgagttata aaaagttgtt atctgaaagg gaagttgtaa gagacaatgc aggattaatg
     6121 gcaattagat ttttagccga tatgaatgtg gatgaaatat tcttagctgg atttgatgga
     6181 tattcacata atgaagatga gaattatggt gagcaatcca tggaaattat aacaaaattt
     6241 gcattattag atgcaatgaa cgtgcaaatg agagatgaat tgtcagaatt agctaaaaaa
     6301 gtaaaggtga catttttaac ggatccacgt tgggttcgaa ttgataagta gtagtaataa
     6361 tgtttttaaa attttgataa agagatagtg agagaataaa aatgggtaaa gtcttaaata
     6421 aaaaagaaga aaaatattta agggccgatg gggcgagatg aaactctaat tttgaacttt
     6481 taagaatcat ttctatattg tgtattgttg cttaccatgc taatttgcgt ttaaattata
     6541 atgcagacgg aattatagta catggttcat taactgctaa ccagattttc ttgaattata
     6601 ttggtatttg gggtcagcta ggagttaatt gttttatatt tatttcggca tattttttaa
     6661 gtgagaaaag aaacattcac tttgaaaatg ttttgggaat tgtattagaa tgtatttttt
     6721 atagtgtatt agtttatgga attctttgtg catgtggaat agaaacattt tcattgaaca
     6781 gctttgtaaa agaaatatta tcatttgctc taggtagata ttggtttatt acatcctatt
     6841 tagcattgta tattctaagt ccgtatttaa atatcatgct taacaatctg gaaaaacttc
     6901 agttgcaaaa aataagtatg ttattgttta taggtgtatg ctgttatcgt atgttattta
     6961 aaacatcttt gtttggggat tgtgctgttt taattgcact atatgtcata tgttatacac
     7021 tgaaaaaaac gataataata tttttgagag acacagaatc ataggaacaa ttgtatgtat
     7081 aacagttata ccacttctca tatccatagg aatacttttc aaatttatac caaatgcaat
     7141 ggttaaccag atggttcata ttggttctcc attgatggtt atagcaagta tatttttatt
     7201 ttatgtattt aaaaattttt cattttacag taagtatata aataaacttg cgagaaccag
     7261 tttaggggta tatattttac atgaaaatca ttatttcatt gatggtatga agtctagagg
     7321 cttaatatgg gatgggatat ttggattagg caaaaggttt ttagataggg atttttggat
     7381 ttggtttata attgcagttg tagttgtgtg cataatttgc atggctatcg atgttgtaag
     7441 agaaaaattt gaaagacaga tttttaaaat taagagtgta aagaacctgt gtaaacttat
     7501 gaatcatgtt ataaatatat acccataatt aataaatgag gagaaaagaa gtgaaagtta
     7561 tatcattcgg agaattatta ttgagacttg cagcacccgg ttattcaaag ttatttcaaa
     7621 gagatagttt ggagactaca ttctgcggag gggaagctaa tgtagcggta tccttagcaa
     7681 tatttggata tcaatcagaa tttataacaa agcttccgga ttcagatgtt ggatatgcgg
     7741 cagctaatgc tttgcgttat tttggagtaa atgttgataa tattatattt ggagagggac
     7801 gcatgggact gtattattta gaaaaaggag cagctcagag accatctaaa gtaatttatg
     7861 acagaaaatt ttctgcaatt tcgttagctg atagtgatga atttaagtgg aaggagatcc
     7921 taaacgatgc aaattggttt cattggacag ggataaatcc agcattgtct gagaatctct
     7981 ttgagacgtc cttagatgca tgtcaggctg ccaaaaaaag aggtataaca atttcttgtg
     8041 atttaaatta tcgatccaag ctttggacat cagaggaagc aaaagaaaag atggaacaga
     8101 tactaccatt ggttgatatt tgtatttgta atgaagaaga tgcagaaaaa gtgctaggaa
     8161 ttaaagctga gcatacaaat attaatagtg gagaattaaa taaagatggc tatgtgcagg
     8221 tggctaggga gattcacaag aagtttggat gtaggtatgt agctacaact ctgcgtagaa
     8281 gttattcggc aagtagaaat ggttggagtg caatgttata cgaagcagaa aaagataaag
     8341 cgtatttttc cacagaatac aacatacaaa ttgttgatcg cgttggtggt ggagacagct
     8401 tttctgctgg tataatatat gcgttatgtg aaaatatgtc cggacaaaag gctattgaat
     8461 ttgcgactgc atctagttgt ttaaaacata ctatagaagg tgattttaat cgaactacaa
     8521 ttgaggatgt atataattta atgaatagcg gtggaaatgg tcgcgtgttg cgttagtcaa
     8581 catataaaga taaatcacag taatttttat ggtgattatc tgtcttagta gtgattagaa
     8641 taacgtgaaa ataaattgtt gagcgtttat actgttttga aaaataaatt taacatattg
     8701 aaaaatacaa atatatacgt atattataaa gcgccgattg taaaattaag ctagacaact
     8761 aaaacagtca tttgtgctac actcaaaata gttccgacca aagaattata aggagtgagt
     8821 gcaaataacc tatacctatc ttacaacgga tgaacttgta ataatagagt cttatttgaa
     8881 aattaaccaa tctgttgcta aaactgcgca ttgcttgaat cgttcaagtc aaacgatcca
     8941 taaagtatat ctgttcttca agcaaagaaa atcagcctta gaata
//
LOCUS       DAZKSR010000110         8911 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000110 DAZKSR010000000
VERSION     DAZKSR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.112"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            433..702
                     /gene="rpsN"
                     /locus_tag="ACG8XG_002530"
     CDS             433..702
                     /gene="rpsN"
                     /locus_tag="ACG8XG_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0774414.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            717..896
                     /gene="rpmF"
                     /locus_tag="ACG8XG_002531"
     CDS             717..896
                     /gene="rpmF"
                     /locus_tag="ACG8XG_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0774415.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            928..1305
                     /locus_tag="ACG8XG_002532"
     CDS             928..1305
                     /locus_tag="ACG8XG_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774416.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1326..1475
                     /gene="rpmG"
                     /locus_tag="ACG8XG_002533"
     CDS             1326..1475
                     /gene="rpmG"
                     /locus_tag="ACG8XG_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0774417.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1499..2482
                     /locus_tag="ACG8XG_002534"
     CDS             1499..2482
                     /locus_tag="ACG8XG_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0774418.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2512..2634
                     /locus_tag="ACG8XG_002535"
     CDS             2512..2634
                     /locus_tag="ACG8XG_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0774419.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2652..4175
                     /locus_tag="ACG8XG_002536"
     CDS             2652..4175
                     /locus_tag="ACG8XG_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0774420.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4199..4438
                     /locus_tag="ACG8XG_002537"
     CDS             4199..4438
                     /locus_tag="ACG8XG_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774421.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4631..5194)
                     /locus_tag="ACG8XG_002538"
     CDS             complement(4631..5194)
                     /locus_tag="ACG8XG_002538"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0774422.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5287..5505)
                     /locus_tag="ACG8XG_002539"
     CDS             complement(5287..5505)
                     /locus_tag="ACG8XG_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774423.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5694..6641
                     /locus_tag="ACG8XG_002540"
     CDS             5694..6641
                     /locus_tag="ACG8XG_002540"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0774424.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6608..6697
                     /locus_tag="ACG8XG_002541"
                     /pseudo
     CDS             6608..6697
                     /locus_tag="ACG8XG_002541"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6830..7237
                     /locus_tag="ACG8XG_002542"
     CDS             6830..7237
                     /locus_tag="ACG8XG_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0774425.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7478..8818)
                     /locus_tag="ACG8XG_002543"
     CDS             complement(7478..8818)
                     /locus_tag="ACG8XG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0774426.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3030 a         1385 c         1779 g         2717 t
ORIGIN      
        1 tgaatacacg aaagaatcag tatgattgaa ttagttcact taatgtctat tcttataata
       61 taaggaataa tcaaagtttt aaaaaattgg cgataaacca gttaaacata aaagattgaa
      121 taaaaagtta tagtttcttt catattgccg ttttaagtag aggaaaagct acttaaaacg
      181 gtatttgtgt ttgattggac taataaaagt gaattcttat ttttcagagc ttaaactgca
      241 tattctttta aatgatttga ttatctctat gtaccgataa attcgttttc aaatagttat
      301 ctattcatat gagttttatt tcatgttatt tattgttgac aagagcaact cttgttggta
      361 aagtatcagt gtcaaaacgt aacgattacg atttgagatg tctaaatcaa aataaaagga
      421 gtataaaggt atatggcaaa aaagtcgaaa attgctaaag caaagaaaca aatggcgatg
      481 attgaaaagt acgcagataa acgtcaggaa ttaaaagccg caggagatcg caccgctctc
      541 gctaaattgc caagagattc aaatccgaat cgtctgaggc ttcgtgatca gacggatggt
      601 cgtccaagag ggtatatgcg aaagttcggt atgtcccgta ttaagtttag agaacttgcc
      661 catcaagggt tgattcctgg agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg
      721 cagtaccagc aagaaaaaca tctaaggcga agaaacggtt aagaagaact catcaaactg
      781 tagtaaaacc agaaatttca tttgacgaag cgaatggtga ttatagacgt agccatcatg
      841 tatctttaaa agggtactat aatggaaaga aagtaattaa aggagcttca aaatagttta
      901 atatataact tttaggaggg aatcaaaatg gaaattattt acccaccttt agttgaagaa
      961 ggattaaagt attatcttga aacgacccag caatcgctag ataaatgcac tttttatcgt
     1021 tcgatggtag agagaggaat tattactgaa acgggtttac cgacccaaca ggcaattgaa
     1081 aatggattag tcaaagatta ctatgaggac caaggcttat catttgatga ttttttaaga
     1141 atctatccaa tttttgaaga gtatgacgag gagttatttc aatgtattga tggttattgg
     1201 gagatacctg ttgatatgaa agaaaattta gtttcacaat tggaatctgg cgagttaact
     1261 tttgaagatg cacaacaaat tcaagcatat cttgaagatc gataagtatt gaaggaggaa
     1321 gatttatgcg tcaaaatatt attttggaat gtgtcgaaac tggagaacgt ctgtatctca
     1381 ccagcaaaaa taaacggaat aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat
     1441 tgcgcagacg ggcaattttc aaagaagtga aatagctaag tggtaaaaag gtgacaaaat
     1501 gggcatacca gttacaatcg tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa
     1561 tcaagccttg caagtctcac cctaccccag ggaagaaatt attataattg aaaatgagtt
     1621 tggtgaagtt gggattgatc acaaattatt agttcatagc gaggaacggg tgttacaact
     1681 aaataatgga tgtatgtgtt gcagtcttcg cagtgattta ttagcaatat tgatttcttt
     1741 actggaaaca atagaagaac atcaacaacc aatttcacaa atcatcattg aaactacagg
     1801 tatagcagat ccccaaccaa tcattcaaac acttttaaca gcgcctcaac taagaggaag
     1861 agtatatatc gatagtttat tgactgttat tgatagtgac agttttgaac ttattgaaag
     1921 agaaccacaa gcccttaagc aacttgtcat agcagatcgc atttttgtgt ctagaaaaca
     1981 aaacttgaat ttatctgcac taaaaaaatt acagagagaa atcaaaagta ttaacccttt
     2041 atccgatatt cgaatgttca cacatagtga aacgattcaa aaaaaggatt tttataattt
     2101 ggaaaaattc aatcatccct tagtttttga aagtgattca tcagaaaatc actataatgg
     2161 caaacactat catcaacatc atcaagaaca tgagtttaaa tcaattctct taacttcaga
     2221 tcaagacata aaaaaagatt ttttattcca attaattgac cggctgattt taaccaacca
     2281 agggagcctg tatcgtttta aaggattgat tgctttgcaa gatcaagatt ttatagttgc
     2341 catacaaggg gtaaacgaac aggttaattt tcaatacacc actcgacaaa aagaccaaac
     2401 cactattatt ctaattggaa aaatgttgga tgaagaaagg atcaaacaga gttttgataa
     2461 attactggcg gaaagcaaat aatgataagt attgattagg ggtaatttct aatggagaaa
     2521 acggatatat ctagtgcata tcgacgactt aaaagcccga atatcaaaac tagaaaaagg
     2581 gcattaaaaa ttatcaaaga acataaacaa aataaaatga agaaattagc ataattaagg
     2641 aaggaaaaag aatgaagaaa atcagtgtag gacttattgg gatagcagct ctaggattat
     2701 tgggagcttg ctccagcact aatgatgcaa aagtatcgaa tgataaagat ggaaaattag
     2761 aaattgtaac aacattctat ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag
     2821 cgaacgtgga tttaatggtt ccagcgggat cagaacctca tgactatgag ccctcagcta
     2881 aggatatggc aaaagctcat gatgctgatg tttttgtata tcataacgaa aacatggaat
     2941 catgggtacc aaaagctaaa gaaagttgga agaaagctgg gcctaatgta gtagaaggaa
     3001 caaaagacat gattttattg ccaggtagtg aagaagaaga tcatgatcac ggagaagaag
     3061 atcaccatca tgaactagat cctcatacgt gggtttcacc caaaatggca attaaggaag
     3121 tttcaaatat caaggatcag ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga
     3181 caaatgctga aaagtatttg actaaactga aacggttgga tgctgactat accactagtc
     3241 ttaaagaagc gaaacaaaaa agttttgtga cacaacatgc agcctttgga tatttagctt
     3301 tggattatgg attgatacaa gttcctattg ctggactaag tcctgaagaa gagccatctt
     3361 ctggtcgatt agctgaatta aaagagtatg ttaaaaaaaa taagatcaat tatatttact
     3421 ttgaaaagaa tgctaatgat aaaatcgcaa aaactttggc caatgaagct gggataaaat
     3481 tggaagtatt gaatcctctt gaaagtctta caaaagaaca aatggataat ggtgaagatt
     3541 atgtttcagt aatggaagac aatctaaaag cgttagagaa aacaactatg gttgctggtg
     3601 aagaggtagt tccagaaaaa gaagcgaagg atgaaaagac agttgctaat gggtacttta
     3661 aagatgccga tgtgaaggat cgcgaattga gtgattatac aggcgaatgg caatctgttt
     3721 atccgttgtt aaaagatgga attttggatg aagttttcga ttacaaagca aaactcaata
     3781 aagacatgac agctgccgag tacaaagatt attatactac tggctataag acagatattg
     3841 atactatcaa cattaaagac aacaccattg attttgtagt caacggtgaa catcatcaat
     3901 acacatacaa atatgttgga tacaaaattt tgaattatga aaaaggaaac cgtggtgttc
     3961 gttttaattt tgaaactgat gatgcaggtg ctggtcgttt taaatatatt caatttagcg
     4021 atcatggaat tgcaccaagt aaggcagagc atttccatat tttctttggt ggagaaagtc
     4081 aagaaaaact ttataatgaa atgcataatt ggcctacttt ctatccagct tcattatctg
     4141 agcatgagat tgctcaagaa atgatggccc attaaaatcc aatctttgga ggaaatacat
     4201 ggtaagaaaa gtattattgt ctatggattc caagaatatt gatgcttatc ggcgttatca
     4261 atttgcaaag caaatgagat taaatgggag tacattagct gatattgctg caactttaca
     4321 tgttagtatt gacacagttc atagagtatt aaaacgggat cctgattgtt gttttatcaa
     4381 ggtcaatact ggaagaagta gacgtttagc aaaacggcat aatagcttgg ccaattaatt
     4441 gataatacca gagtagatca tttttccctt atttggattt gatctgcttt ttattatttc
     4501 ttgaattgtc aaatcaagcg caacacttta tcaaagaaga cttcataagg tgttctccaa
     4561 tctaaacact ttctaggtct gttattcagt ttttcaatca tctgatttat cgttgagtta
     4621 tctaccgtag ctatctcttt cgattttggc aagtattctc gaatgagtct attcgtattt
     4681 taatttgttc attgttgcca agggcatgag gatctggaaa ataaaagggc atattattca
     4741 atacttctgt aactgcgaaa tgattggaga attcttttcc tctatctgga gtgatcgttt
     4801 tgaccgcctc atttggaata ctgcttaaaa gttcaatcat tttctcgctc acataagcgg
     4861 ctttctttct ggggaccttc tttcctgcta aaaggaagca tgattttctg tctgtcaaag
     4921 tcactaaaca agtaccacta gttttgccag ctacaatatc ggctttccaa tgaccaaaat
     4981 cacttctatc atctgcggat tttggatggc catggatcga atgagtaagg caaatcttcc
     5041 cacgagactc gacatgattt tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga
     5101 tcaggccttt attgccgtgt gagaggggtt ctgtatcgaa aagatcattg caaattgcac
     5161 ggtagatcgt gataaaacta attgaaaagt ccatatgttc atgcttcaaa cgattcgaga
     5221 ttttctctgg cgaccactga aaatcgacaa ataaatgacg aacaattgac caaactcacg
     5281 gatcatttag aatgtgataa cgtccgcatt tttttcttcg ataagcataa tttttttccg
     5341 ctttggaagg tgattagttt ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg
     5401 gctatgctga atgtcgttgc caattatact aatagaaaat ccttcttcgt gaagcagaaa
     5461 gattctttct cgtttgagta tagtaagatg acaatactgg ctcataatta ttttccttct
     5521 aggagtgttc tcgacaaact tatactagtt ggatatcgat tatgactcat ttttatgtgt
     5581 tgcacttaaa gtgtaaattc aaggtataga aagaggttcc ctatgaaaaa atacctatta
     5641 caatcttaac tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg
     5701 gtgatcacgg aaaggaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg
     5761 gggtattagt cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga
     5821 atggatgcat ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc
     5881 aattttctga cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa
     5941 ttattcaagc aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag
     6001 agattggtgc cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg
     6061 gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc
     6121 gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact
     6181 tagtgtcaac caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg
     6241 ccaaaataat taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc
     6301 tttttaatat agatgagact tcctattcaa tgggctggtt caaagagtta gagcttggct
     6361 atcaaaatca tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc
     6421 ggttactcct tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata
     6481 ttacaagagc aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg
     6541 aaacatcggg tagccataaa agtattgaat cattaaatta ttgggtagct agtttaccta
     6601 aaattgagtg tcaaatgatt atacaaaaaa acccgaattg aaggaagcct ggcatccgtt
     6661 gtttggtgaa cgcattaata aactagtttt tattggtatg aagatggatc aacaatgtat
     6721 taaggaacaa cttgagggtt gtttaactga ttctcttaat gcagataatt tccgaaaaat
     6781 ttcaccattt ccatggaaaa attctaatat gtttagccag atatcgttaa tgaaattttt
     6841 aagaaaagct ggtgtcaaga taggcgatgt cataattatt ggcattttga ttattccctc
     6901 ttttattccc ttatttgtat tctctataga aaaggagaat gaaacaactg aagatgctat
     6961 caaatatgcc atcgttcaaa ttgatggcaa ggaaatggat cgcttcgatt tggataagat
     7021 caatcataaa ctcgtgaatt atcatcctgc gaagggacaa tataatattg ttgagattaa
     7081 agatggaaga attcgagtta aagaagataa tagtcctgat caaatcgcgg ttagaactgg
     7141 atggatctca aaacccggtc aaacgagtat ttgtttaccc cataagttag ttattagtat
     7201 tgagaaaaaa gaaagcaaag attactacat ttactaatct ggaaatagat gaggaggttt
     7261 ttaataagaa aaagatatta ttattaataa tagcaggggc tttattaagt gtgttaagcg
     7321 cttgtggaca agaaaaagaa gcggtgacaa caggcacaag tatctttaac gggacctgca
     7381 atggtttagt taacagtcgg ttagattact tattttggct cttcgtcaat gagtgttggt
     7441 gaagaaattc aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc
     7501 gtgttgttgt tctggcttta gtcagttggg tagtgttctt agttttcttg aaatattttc
     7561 ctcgatataa aaagattcgt tctcggtagt tttgaaagtt tctaaagcca taggcaattc
     7621 gttttagtac tttgatatgg ttgttcaaac attctatagg gccgttggaa tagggaactc
     7681 gcaacgctcg tttgatttct gttcgatatt tcttgaaggt cgtgaacact ggttgatagt
     7741 gctctggcaa ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc
     7801 ttctgatagc ggaaagaaaa acctggtaga cttcgtagcc tcgctttaag gagtcatcat
     7861 aatataatag ccgatcgact atctccgctt ctgtcagatg atctcgaaat gacggacgcc
     7921 agtagcgatg ctgaccattc aattcccacg cattcttttg aagtagtctc cagtatttct
     7981 ttaagtgctt cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt
     8041 tggtaaaggc ccgaccgatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat
     8101 tcggaaaaca ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac
     8161 gtacttgttc acggactttt ctagggtagc gcaagaaata gtcgcgtaaa ggattgagtt
     8221 gccgattttc cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca
     8281 tggctcccga gacgtttttc accgatttga attcatcgaa acacaccact tctggtaacg
     8341 gctggttcaa agaaacggtt ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg
     8401 gagaaatgtg cttcatacgt gcaatcaaag acatagaaat aggctcactt aatagctcaa
     8461 gaatggcttg tttgactctg cgggcaatgg agcagcgacg atcgctaact gaatcttctg
     8521 ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg tttgttaatc cgcacaatgg
     8581 tcttgtagtt actgacatca ttcaataaga tggatacttt tttccagccc catttaatga
     8641 tcgtttgctt attttgatag tggcaatgga agcattcctc tggttgatag gtcaatgtgc
     8701 catcaaaaac aaaacatgtt tcgtcattaa tgcgctcttt gcgaaaggca tcttcatgaa
     8761 aggtaagatt taggtctaga atatctagta actctttggt aaactctgaa taggacatct
     8821 tgaaacactc ctttgaatgg ttttcgtcga ctttattcta aaggatttca aggtgtctgt
     8881 ttgcgtttat tagaaaaaag aaaaagtatt g
//
LOCUS       DAZKSR010000111         8749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000111 DAZKSR010000000
VERSION     DAZKSR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.113"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..1116
                     /locus_tag="ACG8XG_002544"
     CDS             109..1116
                     /locus_tag="ACG8XG_002544"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0774427.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1128..2132
                     /gene="neuB"
                     /locus_tag="ACG8XG_002545"
     CDS             1128..2132
                     /gene="neuB"
                     /locus_tag="ACG8XG_002545"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0774428.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2129..3277
                     /gene="neuC"
                     /locus_tag="ACG8XG_002546"
     CDS             2129..3277
                     /gene="neuC"
                     /locus_tag="ACG8XG_002546"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0774429.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3250..3927
                     /locus_tag="ACG8XG_002547"
     CDS             3250..3927
                     /locus_tag="ACG8XG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0774430.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3878..5059
                     /locus_tag="ACG8XG_002548"
     CDS             3878..5059
                     /locus_tag="ACG8XG_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0774431.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5072..6049
                     /locus_tag="ACG8XG_002549"
     CDS             5072..6049
                     /locus_tag="ACG8XG_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0774432.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6049..6957
                     /locus_tag="ACG8XG_002550"
     CDS             6049..6957
                     /locus_tag="ACG8XG_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0774433.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            6958..7710
                     /locus_tag="ACG8XG_002551"
     CDS             6958..7710
                     /locus_tag="ACG8XG_002551"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0774434.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            7715..8662
                     /locus_tag="ACG8XG_002552"
     CDS             7715..8662
                     /locus_tag="ACG8XG_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0774435.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3216 a         1104 c         1676 g         2753 t
ORIGIN      
        1 ttgattcgtt acgttttgac atcattattg ataattagtt tagttatata ttttttaaag
       61 attaagaatc gaattataga tgactataat tcataggagg agatataagt gtttaaagga
      121 aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt tcgtcagctt
      181 ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa acaattttat
      241 atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga tatcagagac
      301 tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc tgccgcaatg
      361 aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa tgtgatggga
      421 actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt aggtatcagt
      481 acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt gagtgaaaaa
      541 ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc ttgtgtaagg
      601 tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa acaaataaaa
      661 aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat gtcaataccg
      721 gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga aacatatgtc
      781 ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa aatatataat
      841 gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag gccaggagaa
      901 aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga aaatgataaa
      961 atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa
     1021 attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca ggaatttatt
     1081 agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg aaatcagaag
     1141 tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta gctttagaaa
     1201 gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca tttaaaacga
     1261 ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat cttaaagagg
     1321 acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat tttattagaa
     1381 taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt gatgaagaaa
     1441 gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca ggagatttaa
     1501 caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta ttatctacag
     1561 gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat agtccagtta
     1621 ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat ttattggcga
     1681 ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat tcattgggat
     1741 atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa cattttacct
     1801 tggataagga actacctggt ccagatcata aagcttcttt agatcctcag gaatttgcag
     1861 attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt aagcgagcaa
     1921 tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc actgagaatt
     1981 taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct ggaaatggaa
     2041 tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca ataagtgcag
     2101 atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa gtggaacacg
     2161 agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata attgggatgt
     2221 atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta aagaattgat
     2281 cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag agaacaataa
     2341 gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata tatttgaaca
     2401 aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg cagctgcgat
     2461 agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag tgtctggtac
     2521 tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt ttgctgctac
     2581 ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag tttttcccgt
     2641 cggagctcct agaatagata ctattttaaa taatagatta cctagtttta gagatataaa
     2701 agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat ttcatccagt
     2761 tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt caatggaaca
     2821 acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg aaattatcag
     2881 aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt tagagccgga
     2941 ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt cttctgggat
     3001 aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac aaaatggacg
     3061 agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg aaactgcttt
     3121 gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga acatctacgg
     3181 tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga atgatgggga
     3241 agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga tccatacatt
     3301 attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt agaaaataag
     3361 ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa atatcttggg
     3421 aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa atttattgta
     3481 gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat gccaagtgag
     3541 caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc aattggttat
     3601 ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg agagcatgct
     3661 ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta tgttcattta
     3721 tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca aataggaatt
     3781 ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg cgcagggagc
     3841 actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc taaaattaag
     3901 aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata tgggaggaac
     3961 agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat taggtgaaaa
     4021 tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg gagcttctgc
     4081 gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa
     4141 cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta ttttatatca
     4201 aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga gccctgttgc
     4261 actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg caatcatcgt
     4321 agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg caaaccaatt
     4381 taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata agggaatttc
     4441 gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga tcatcacaac
     4501 ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt caagattttt
     4561 agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag gttataatta
     4621 ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat tgaatgatag
     4681 agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg gaattgatag
     4741 aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt ctactttaac
     4801 gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac taaatgaaaa
     4861 aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat ttcaaggggt
     4921 aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta atagcggctt
     4981 atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta ttgatgagtt
     5041 taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg gtttaggttc
     5101 catggggaaa agaagactta gactcattca agaacacttt aatgatattt tactatgtgg
     5161 tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa taccaactta
     5221 taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg tttgtacatc
     5281 ccctataacc catgaattta ttataaaaga ggctttaaat ttagattgta atatctttac
     5341 ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtagcta aaaagaaaga
     5401 tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat atattaagtc
     5461 taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt atttacccga
     5521 ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa ctaatgggtg
     5581 tagagaaata ttcgctattg aattaccttg gattatagat acatttggga aagttaaatc
     5641 gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg acagtttcac
     5701 tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg ttgtttcaag
     5761 agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat gggaaggtaa
     5821 cccggattct ctattttcat ggaatgaaga aacttctagt atgacagata ttcaattgta
     5881 cgatgaggtt atgcatgata ataattattc acatactatt attgaagatg catatttaga
     5941 ggagattaaa gaatttttca cgcttttgga aaagaaaatg gaaccccaat atacttttga
     6001 aaaagataag tatattatta acttaattaa cgacattgag gtaaattaat gatgaaagta
     6061 attttttttg ggttagggtc aattggaaag agacatctaa aaaatctaat aagctatgga
     6121 aaaaagaatt ctattaattt tgatataaca gcttatagta gcagtggaaa aaaagagttt
     6181 gaagaaataa cgtatattca aaatagagac gatttggata cacattatga tatagctttt
     6241 attacaaatc cgacttttaa gcatctggaa acactaaatg taattgttga aaaagttgat
     6301 tacatatttt tagaaaaacc tgctttttct gagagtattg atcttactca atataattat
     6361 gattacaaaa atatttatgt tgctgctcca ctgaggtata aaaatatcat ggaagttgtt
     6421 cgtaatagta ttcaaagtaa aaaagtatat tctgtaagag ctatttgttc tacttatctt
     6481 cctgattgga gaaaaggtac tgattataga aataattatt ctgcctttaa atctatgggg
     6541 ggaggagtag aattagactg tatacatgaa ttggattata ttataaacct atttggtttc
     6601 cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc ttgaaattaa cagtaatgat
     6661 ttagcaacat atatattgag atacgatgat aaaattatag aaattcattt agattatttt
     6721 ggaagagttc ctacaagaaa atttcaattg tttactttag atgaatgtat caatgttgac
     6781 ttattaaata ataaaataag ttttgaagaa aacattgtta aagaaatgcc tgaatctgct
     6841 aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac aggcaaagaa
     6901 aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga gaaataaatg
     6961 gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa taaaaatttg
     7021 aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga tatctatcaa
     7081 caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa tgagttagta
     7141 gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc tgaattagct
     7201 acagatactg tagggaaaat tgaagtgata cgtgattcat tcttacaact agaaagagaa
     7261 gataatatat ttgatttaat tatagattta gatttgactt cacctttacg tgaagtttct
     7321 aatcttgaag aattaataga tgaacatcta aaaaatttag ataaagacgt ctacttttca
     7381 gttgtttctg caagacgtaa tccctatttt aatatggtaa aaagaattga aaatgaagtt
     7441 actatagtta ataaatcaag atacacatct aggcaacaga cacctgaagt ttttgaaatg
     7501 aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa aaagtaatta tatttttgat
     7561 gggcaatgtg ggataataaa aatgagagat tatctaattt tagatattga tagtgaagaa
     7621 gattttaaat ggttagaatt tttgtttcct aaatttttag aagattcttc ggagcttcaa
     7681 gatatctata attatttata tcggaggtaa aaggatgagc tgttatctta tcaccggtgg
     7741 ggctggcttc attggttcta ccttagccaa tcatttagga gaagaacatg tggtaatcgt
     7801 cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat atgacgaaaa atatcacttt
     7861 tattgaggga gatgtcgctg agaaatcctt gatggaagag atcatgaaag ctaataaatt
     7921 tgattatatc tttcatttag cagcagttgc tagtgtggca gattcagttg cccgaccagt
     7981 agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt ttagaattga tccgcaagta
     8041 tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg gctgtttatg gggatgaacc
     8101 gactttacca aaaaaagaag agtccgtgat tcgcccattg acgccttatg cggttgataa
     8161 attcgctgca gaacaatatg tgctagatta ttgtcattta tatgatgtac ccggtagcgc
     8221 agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg aattctccat acagtggtgt
     8281 gatttctatt ctcgtagatc gctataagaa acaaatcgta ggagaagcaa cttcatttac
     8341 attatttggt gatggaagcc agtctagaga ctttgtttat atagatgatg taatccaagc
     8401 attactttta gttgcgaatg aggaagctgc actggggaaa caattcaatg ttggaacggg
     8461 taaagcaacc acacttttgg aattgattca tgcaatcaat gaaattctcg gtgtagaatt
     8521 gacacttgaa tacaaagaag agcgttcggg tgatatccat gactctctag cagatattac
     8581 taagatccag tcaattggct atcaaccgaa atatggtatt ttaagtggaa tgactaatta
     8641 tttgaaaact gaaatcaatt aaaacatact aagataagat tagagggtaa attcaggctc
     8701 tttgtcaata aggactgatg agttttgtaa aatcaaatct gggatagaa
//
LOCUS       DAZKSR010000112         8493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000112 DAZKSR010000000
VERSION     DAZKSR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.114"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8XG_002553"
     CDS             complement(<1..149)
                     /locus_tag="ACG8XG_002553"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774436.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8XG_002554"
     CDS             complement(593..1714)
                     /locus_tag="ACG8XG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0774437.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8XG_002555"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8XG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0774438.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8XG_002556"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8XG_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0774439.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8XG_002557"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8XG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0774440.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8XG_002558"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8XG_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0774441.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8XG_002559"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8XG_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0774442.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8XG_002560"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8XG_002560"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774443.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZKSR010000113         8250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000113 DAZKSR010000000
VERSION     DAZKSR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.115"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8XG_002561"
     CDS             234..1559
                     /locus_tag="ACG8XG_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0774444.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8XG_002562"
     CDS             1552..1902
                     /locus_tag="ACG8XG_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774445.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8XG_002563"
     CDS             1899..2069
                     /locus_tag="ACG8XG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774446.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8XG_002564"
     CDS             complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8XG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0774447.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="ACG8XG_002565"
     CDS             complement(3220..4173)
                     /locus_tag="ACG8XG_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0774448.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="ACG8XG_002566"
     CDS             4785..6278
                     /locus_tag="ACG8XG_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0774449.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="ACG8XG_002567"
     CDS             6413..6709
                     /locus_tag="ACG8XG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0774450.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="ACG8XG_002568"
     CDS             6810..7661
                     /locus_tag="ACG8XG_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774451.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7894
                     /locus_tag="ACG8XG_002569"
     CDS             7682..7894
                     /locus_tag="ACG8XG_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774452.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2864 a         1283 c         1553 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgacga
//
LOCUS       DAZKSR010000114         8208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000114 DAZKSR010000000
VERSION     DAZKSR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.116"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1056)
                     /locus_tag="ACG8XG_002570"
     CDS             complement(<1..1056)
                     /locus_tag="ACG8XG_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0774453.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEP"
     gene            complement(1210..1740)
                     /locus_tag="ACG8XG_002571"
     CDS             complement(1210..1740)
                     /locus_tag="ACG8XG_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0774454.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1839..2189)
                     /locus_tag="ACG8XG_002572"
     CDS             complement(1839..2189)
                     /locus_tag="ACG8XG_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0774455.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2346..3875)
                     /gene="ahpF"
                     /locus_tag="ACG8XG_002573"
     CDS             complement(2346..3875)
                     /gene="ahpF"
                     /locus_tag="ACG8XG_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0774456.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3885..4448)
                     /gene="ahpC"
                     /locus_tag="ACG8XG_002574"
     CDS             complement(3885..4448)
                     /gene="ahpC"
                     /locus_tag="ACG8XG_002574"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0774457.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4592..5254)
                     /locus_tag="ACG8XG_002575"
     CDS             complement(4592..5254)
                     /locus_tag="ACG8XG_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0774458.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5438..7084
                     /locus_tag="ACG8XG_002576"
     CDS             5438..7084
                     /locus_tag="ACG8XG_002576"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0774459.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7075..8157
                     /gene="uxuA"
                     /locus_tag="ACG8XG_002577"
     CDS             7075..8157
                     /gene="uxuA"
                     /locus_tag="ACG8XG_002577"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0774460.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         2701 a         1583 c         1476 g         2448 t
ORIGIN      
        1 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
       61 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
      121 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
      181 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
      241 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
      301 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
      361 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
      421 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
      481 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
      541 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
      601 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
      661 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
      721 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
      781 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
      841 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
      901 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
      961 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     1021 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     1081 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     1141 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     1201 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     1261 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     1321 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     1381 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     1441 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     1501 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     1561 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     1621 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     1681 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     1741 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     1801 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     1861 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     1921 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     1981 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     2041 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     2101 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     2161 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     2221 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag
     2281 gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt tacaatcttc
     2341 atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc
     2401 attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc gaaaacgccg
     2461 gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt catttcgatc
     2521 gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac tcctgaaaca
     2581 gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc tacctgattc
     2641 gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc aagagagact
     2701 agtttatcct gtaagatttg atctgctttt aattctggta agaattctag aacatataca
     2761 tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc accaccgata
     2821 acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata agcaattcct
     2881 ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg
     2941 atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt aataaaatgt
     3001 tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg gtaactacgc
     3061 acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt accaatcata
     3121 ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac aagtcctgta
     3181 cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc accgccaatc
     3241 actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg gccagtgatt
     3301 ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc atttaaaaac
     3361 acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat cccaccttca
     3421 atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc ctgtacaacg
     3481 tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt ttgtgttatt
     3541 tttttgattt taagactcaa agattcactg attttcggtg cccgtccact aatttgtaac
     3601 atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa aacgattcca
     3661 ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc
     3721 caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag tttttgacta
     3781 ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag tgctaaatat
     3841 tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat ctttccaaca
     3901 agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg acaaacttca
     3961 cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc actagcgtca
     4021 cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg atcaataata
     4081 aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat acgagaaatc
     4141 tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc tgatgtatca
     4201 tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc aacacctaaa
     4261 gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca aacaaaagta
     4321 aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt ttcactgact
     4381 tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt tttcccaatc
     4441 aatgacatag taaaacctcc aataataatt atttatttat aattattgta atttagttgg
     4501 agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa cgaaggttaa
     4561 ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg ggataacgtt
     4621 gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag
     4681 cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt tccatgatcg
     4741 tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct aaatgggtat
     4801 tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa caagcttcat
     4861 gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact gctttttctt
     4921 gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg gaacaacatt
     4981 caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct gcagaaacaa
     5041 gattaatttt tgaaacatac gtaccacttt gaggtatcgt atacacgagt tcttgctgac
     5101 gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt tcctccagtg
     5161 ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt ttacgaatcg
     5221 cttgataagc ttgagattgc aagctgcttt tcatctttat tcctccaaaa tcatttctat
     5281 gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt aactagtaaa
     5341 tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa aattcacttg
     5401 aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa acaactggag
     5461 gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg accgtgcatc
     5521 attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg gaaatcttta
     5581 tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat tgtccactgg
     5641 tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc aagattttga
     5701 tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac gtatattagc
     5761 tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg ataagatgat
     5821 tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg aaaaaggtta
     5881 tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata taaaaaatgg
     5941 tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata aacgtttttt
     6001 atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa acggtagtca
     6061 attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa
     6121 tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga cgatgattga
     6181 tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc
     6241 aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg tcaatacaga
     6301 ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga aattcgaaaa
     6361 agctggagta ttcttgactg atcgtgcaac ggtagataaa gcagatacga tgaaggtgac
     6421 gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac tggggtatag
     6481 gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg aaatcgggta
     6541 caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga tctttctgga
     6601 agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc agcgaatcgc
     6661 cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa aatatatgga
     6721 aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg ctgcttggct
     6781 tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc cagacccact
     6841 attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag atacatcaca
     6901 tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg tagatcttta
     6961 ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag caggaccgaa
     7021 tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt tatcatggaa
     7081 atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga aatcagacag
     7141 attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg agaggtatgg
     7201 cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt aacactaaaa
     7261 gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc ccgtgagcag
     7321 tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat agaagtcatc
     7381 tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta ccgcttagaa
     7441 gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc aagagaaatc
     7501 gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga acctgagcgc
     7561 ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga aaaacttcga
     7621 gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt aggggtcaaa
     7681 atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg aatcgtaaag
     7741 aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa cggaatcact
     7801 ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat tttagcagaa
     7861 ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt tttagctaat
     7921 cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat gtaccgcata
     7981 ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca tggaagaatg
     8041 atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt aggtgctgtt
     8101 tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga taattagtga
     8161 aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattctt
//
LOCUS       DAZKSR010000115         7941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000115 DAZKSR010000000
VERSION     DAZKSR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.117"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2670)
                     /locus_tag="ACG8XG_002578"
     CDS             complement(<1..2670)
                     /locus_tag="ACG8XG_002578"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0774461.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            3238..4458
                     /gene="sstT"
                     /locus_tag="ACG8XG_002579"
     CDS             3238..4458
                     /gene="sstT"
                     /locus_tag="ACG8XG_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0774462.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4605..5240
                     /locus_tag="ACG8XG_002580"
     CDS             4605..5240
                     /locus_tag="ACG8XG_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0774463.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5431..6171
                     /gene="truA"
                     /locus_tag="ACG8XG_002581"
     CDS             5431..6171
                     /gene="truA"
                     /locus_tag="ACG8XG_002581"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0774464.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6316..>7941)
                     /locus_tag="ACG8XG_002582"
     CDS             complement(6316..>7941)
                     /locus_tag="ACG8XG_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774465.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2625 a         1234 c         1491 g         2591 t
ORIGIN      
        1 tttttgtgtc gcccatgttg cactgtactg gttacctgca acaagatcat attgtatcag
       61 acgataatct tctagaattt ctttttgttt ctcagttaaa tttttttctt ggataatttg
      121 actatcctct gtaacaaaga cctgttttcc attaaaacga ttagacaagc tttctgtagg
      181 atcaattgtt aatgcaggta gatgattaga tacttctgtc aacaacgcat agtaaggagt
      241 tactttgata ttcgcatgct caagagccat agcaccaaag ttattaggag caacaacttc
      301 tttatttaac gtagctgctt gttcacgact atacttgttg ttataaatga aataatctgt
      361 ctggtgctgt tctaatccat attttttcga atcattccca ctatagattg cgggtaaatg
      421 gtcaccataa aaaacaaagg taatcggttt atcgattttg tcgatttctt ggatgaattt
      481 ttttactgct gtatcggtat agtgaaggcc ttgcatataa gtttgtaatt cttttcttct
      541 attaggaatg acagcagttc cttcagcagt gtagttcaat tcactatagt aatcatcata
      601 aggcatatgg ttttgcatag tggataattg gatgaattgt gacgtttctg tattgctttg
      661 taaagctttc aaggtttctt tgtatgctga atcatccgat atgcgtgggc tttgttctat
      721 tttatccgta taagttaatt tatccggact gtctaaataa tagaacctgt caaaaccaaa
      781 tttatcaaac acttgttttc tgcgataaag agcagctgta aaaggatgga tggctatttt
      841 ttcatcgaat agatcggtaa tatttggact tattttctga cgctccacta gctgggtata
      901 aggtgtaact aacgaagcag acaggttact gatactcaag cctgtcagtc cttcccattc
      961 catgttagct gttccgccac cataaccgac ggataacatc aatcctcctg ttccttctgt
     1021 tataatgctt ttgatgtaag gaatagggtc tgtaggtact gtcagattag ggacacgtgc
     1081 aggatcactg aagctttcac ttaaattgag aacgatcgtc tgattgttta accagtcact
     1141 tcttgtttgg ttgatttcat ctgcctttgt gttgtacttt ttcattattc cattaatttt
     1201 ttctttcgaa tagccagcag gttcttccat gattttaatg tcgatattgt ttaggaattg
     1261 aatgactggt ccgttatctt ttacggcgcc ttgctggtta aagaaagtct tgtttacttt
     1321 aaataaatta aacagcaaga aagaaggcga atttttgtga ttaatgaaaa agacacccga
     1381 aaaacatcct aataatacag cgatactgat aaatcgacgt ttccaatgaa tcttcaagga
     1441 atactgtttt tgtagtctac gttgtacaat aatactagta atcaataata ggagaaggat
     1501 caatccgccc acgattaata agactgggct aaccatcgat aatagttctg acaatccggt
     1561 cagtattttt aaatctgaag gaaagacagg ctcttcccgc atttccattt tgattactgt
     1621 cgaaatagtg agcagtaagt taatgatcaa ggtagcagtc aaggcgtacc aaaaacgatt
     1681 ggaaatcaac aatagtaaga agaaaaatcc cataataatg atgattgtca ggatcatggg
     1741 tgcttgtcgc tgagtaaata tttttaaggc aacctggata gtttctttcc aaccttcttt
     1801 tggttctaaa acgaaaatct gcacgaaggt aaagcagtaa aaaaatgctg caactaaaaa
     1861 ataactttta tgaacaaaaa gccaatgaat tctttttttc caacgctgag ttaattgttc
     1921 aatcgaatca aaagcgaagt attcgatgaa acgatgaatg aaacggattt tttgtatata
     1981 aaataaaaag ataactagga gggacactgc taataaatag caaataaaaa actgcccgat
     2041 atttatcagc catgccttcc ctgagtttgg aaattcacgt ttcataaatg tagatacgcg
     2101 ataagaaact aaagggaaat tacatataac ttgagtgaca atcaaaaata cagcaagaaa
     2161 atttcctctt ccttttatac ctaatttatc agatagcatt tctaaaaata aaaacgaaga
     2221 gaaagtataa tacattgcga agagagaata tggtttagaa aacctcagcg ctgtatctgt
     2281 atttcctctg atgatgagtg aaatttgtgt catcaaaata atcaaaccga ataaaacaat
     2341 caagctgata aatacttgag gaagcttata gcggaccttt tctttccagt tccattcttt
     2401 caaaccataa cccattaaaa ataaataaaa aatccataaa atactctgtc cattctgtaa
     2461 tgcaaacggg tctttattaa atagagtaga gcagccaact aatccaattg ttaaaaataa
     2521 tagtagatat ttaattgttg ttttcgataa tttatttaaa aatccaactg tataaggaat
     2581 actacaaaaa agcaaagtac aagaaacagc ataagtaaag taattctgac tgataggtgt
     2641 taggataagc caataatcac gaatattcaa gctgccagct tgtacgtaaa aaaaagatat
     2701 tgcaaactag tgtggctatt aggaatataa aggcaaaacg acttaaataa tgtaaaactt
     2761 ttaaacgggc gattttttga tattggtaac ccatgtaaag tgggatcaaa tttgcaccaa
     2821 taacaacgga agtttgtaaa aatgtataga tgtagaatcg accagggttt gttgcatatg
     2881 taggaatagt gactgctgaa atcaatatac gctgtaaaac catcattata caagcgacaa
     2941 agattgtcag gaagccataa ctctccttct ttattttttt caagaataaa cacctcacaa
     3001 aaaaattgga caaaaacata agaaaacgtt tcctgataag ttgcccatta ataacatcct
     3061 aacttagaat atctcaaaaa acaacgtgta gacagaaaaa gtacaaaatt agcaagaaat
     3121 ggttgtttgt attgttagaa tttttagaaa attatacctt tagtttcttt gcataaaatg
     3181 atacaatgat tgttaaaatt gtgaaagggt ggtcatttgt tagagaagtt tttggatatg
     3241 tcgttagttc aacgtattgt tgttggtatt ttagtcggaa tattgtttgg tatctttttc
     3301 cccacatgga catttatttc aatattggga acgttgtttg tcagttgctt gaaggcaatc
     3361 gcacctattc tggttttttt cttgacgatg gcgtcaattg ccaagcataa aattgggaat
     3421 aagacattcg tgaagcctat tttgatctta tatttagtgg gaacattgct ttctgcactt
     3481 gtagcagtta ttgtaagttc aatttttact gttcctatta ctttacaaga aacagtatct
     3541 gaaaaagcac cgcaaagttt ggaaagtgtt ttatctacta tgttgacgaa tgtcacacaa
     3601 aatcctgttc aatctcttat tgatgcgaat taccttggcg tcttattctg ggctgtattg
     3661 ttgggactag cttttcgagc acgttcagaa tcgacaaaag agttggtgga tcagatatct
     3721 atagcgttgt cacaagtcgt acaagtgatt attgcatttg ctccgatagg gattttagga
     3781 ttggtttatc aatcaattgc tacgactgga atagctggat tggcagagta tctgcagctt
     3841 ttacttgttt tgatcggtac gatgctgttt gtcgcattag ttgtgtaccc attcctcacg
     3901 tttttgttta ttaaagaaaa tccctatcca ttgattttct tttgtttgaa agaaagcgca
     3961 cttcccgctt tctttacccg aagttctgcc gcaaatatcc cgataaacat gatgttggct
     4021 gaaagattga aattgacaaa agagtcttat tccatatcga tcccgttagg tgctacgatc
     4081 aatatgggcg gtgccgcgat taccatttcc ttgatgacgt tgactgctgt acattcttta
     4141 gggattgaag caccttttgt tttgaaagtc cttttatgta ttttgtctgc tttagctgct
     4201 tgtggagctt caggtgttgc gggcggttca ctgttattga ttccattagc atgtagtttg
     4261 tttggtatct ctaatgatgt cgcgatgcaa atcgtcggta ttggatttat tatcggtgtt
     4321 atccaggatt caatggaaac tgccttaaat tcgtcaagtg atctgttgtt tactgcaata
     4381 ggagagctag gcgcgcgtaa aagaagtggg gaaaaaatca aattgaaaga ttgtttagat
     4441 ggattaagtg aacgttgaac gttagatatt tagtattttt aaaatgattc gagtttttaa
     4501 tgagttatat agatgttcgt gtaaatagag gtttctgtta aatcaagata aagtcgctta
     4561 taataacaat taaaagcata ggtaattgtt attggaggcg tatcatggat tttttacgta
     4621 aatttggttt tttcttttta ttattaggcg tgctgagtga ttttttgact ccgtacgtat
     4681 taggattgta ttatcctgaa ttagatcaaa tgaagagcgt gatcagtgta tttggagagg
     4741 tttccagtcc tgtaagaaaa gcgtttctta tttggtctgt ggtttcaggg atctttttca
     4801 tcctgtcatt gcctgcattg tatgctgcat ttgtaagtac atctcgtgtg ttagctaccc
     4861 tagttacagc agccatcgcg ttctatggta taggtgattg tatctttaca ggtttgttca
     4921 gtatcaatac ccatgaatct tcgtggaatg tctcgacatg ggtccataat atcggatcag
     4981 gtttaggtta cagtggtttt ttattgtttc cagttcttct tttcttgatc tatcgaaaaa
     5041 atgggcaaat ggacgtaagc cacttatacc ttggactgtc tgtgatcagt ttgctttttg
     5101 cggcgattta tggactggca agaattcctt ctataaattc ttggccatta ctgaataaaa
     5161 taggtttttg ccaaagaatc agttttttct ttaattatgt gcccattgcc tggttgagta
     5221 tcttgcatct caaacagtaa aacttgagcc gtcaaattga acagtcagcg gatacttcat
     5281 ccggtggctg ttttttgttt ttctgaaata tatatttcca cagttttctt gtatagcttc
     5341 tgttttagag aagaaaggag ctatattttg tgctacaatc ttaaaaaggt agagagaaga
     5401 ccaaatggaa gacagaggag ccactaaaat atgagaaaca tcaagttgac aatcgaatat
     5461 gacggaaaaa gatattcagg atggcaaaga cttggagatg acgataaaac cattcaagga
     5521 aaaatcgaaa aagtattgca tcagatgaca aatgaagaga tagaaattat tggatcagga
     5581 agaacagatg ctggcaccca tgcgcgagga caagtagcaa attttaaaac aaacacagaa
     5641 attggactgg cagaaatgat cgactttatg aatcgttacc ttccgcggga tattgtcatc
     5701 aaaagaatcg aagaaatgcc tgagcgcttc cattcaagat ataatgcggt gggcaaaaaa
     5761 tacagctatt atgtttggaa caacgtgatt ccttcagcat ttgagcgaaa ccacagcttt
     5821 tatttccctc aagagttgga tatggacaag ctgaatgcag cttgcgaaaa attagtgggt
     5881 acacatgatt tcattggttt ttctgcattg aaaaaatcaa aaaaatccac agtgcgtacg
     5941 attgatgaat tatctatcga acgaaaggga gaaatgcttc attttacttt tgtcggagac
     6001 ggattcttat ataaaatggt gcggattatc atggggacat tactagaaat cggcacaggt
     6061 gccatgccga tagaaacaat cgatgaaata tttgaaagca aagtgcggca acatgcagga
     6121 gaaaccgtcc ctgcacaagg tctgtttttg gatgaagttt attatcgata aaaatacttt
     6181 gataatggat gaaacgcatg tttcacagta taaatatcga tgttaaataa tgtatgaagc
     6241 aaattaaaga aatataaaaa aagacttttt agcctgttta tgtgtacagc taaaaagtct
     6301 ttcttaaata aaaatctagt gagaatgttg tacttctctt ctcggttgtt tttgtttaga
     6361 aaaagtaaat cgataaagta aaaataaaat caaagtaacg atagataaat atcttgaagc
     6421 aattgctagt tttgtgtact gataagtaat aacgacttta tttatacctg gaggaatagt
     6481 taattcagtt gtgccaaact tagataattt tgtttgtact agtttgccat taatagaagc
     6541 cacttgccct ttataacgat aaataggtgt cgtcaagaca gtatttttat tattcgaatt
     6601 atctaattca attgtaagtt tgttactcat aaaatgagtt gttggattta tttcctggtt
     6661 acctagtaaa tattgatcat tattaatcat atcaggatga tagattgact ctttgtttgc
     6721 ataatcagta tgattgtaat tttcagctat agcaacagcg tcttcaggtg ctataagtgt
     6781 taatgaatct ttgttagcat ggatagaatt gcttactcct gagagatgca atattagtgc
     6841 aagaaacgta aagaccatta ttttccatga attattttta gtagaaaaaa tataatagat
     6901 cactatactc atcgtatagg ctataaataa agaagaaaaa ccatttaacc gccagacaaa
     6961 ttgtagactc ttaactggtg tatggttaaa aagttgccaa ggaaataaat tggttgaaca
     7021 aaaaagaaca aatacaccaa gaaagaaaat aaataaatca tcagcagtta atttttttat
     7081 aaaaataaaa gcaccaatca atccgagaaa taagaataat ccaatagtat agttgttata
     7141 gttattattt aaaatgtgtg tcagcatatc actaggagcc ataccactta attcttttcc
     7201 cagtgggact ttcaattctt gtgctctaga ttgttgaata aaaggaatta agaatcccgc
     7261 acttaaaaaa aaggtgagga cggtagcttt taaaagactt aagagtctag cgattttttg
     7321 gtcccaaaaa taaaaagaaa gaaacaacgt tcctccaata aatacagaaa ccatagcgac
     7381 agaaagtaaa tgagtataca ctactagcgt catgccaata gacaaccagt accatttttg
     7441 gtaatctcta atataaattt cgtaacaccc cattagtatc aaaggcaaaa atgttaaagc
     7501 gacagcttct cccaatgaag cacgatggaa tatgtcaatg gcacgatagg cggaaaatgt
     7561 ataaataatc gaaaataaca gcgaaatata acgattgttt tttatttgtt tcatagaaaa
     7621 ataagaaacg accattgtta aaaaagtgat aaaaaaatag tagatattat aacttaaaac
     7681 aagatttccc atcattttat aaaataaaaa agctggataa agagtaagcc atggataaaa
     7741 aatattcatc attgttccat gatgatcgaa gttattgaag tttactggag aactccatac
     7801 attatctagt cctatgattc gagataaatg aaagtaagta tcctgattga cactatgata
     7861 gattcccata gacatgaata gagggatgac gtaagcaaaa cttagaaata acataaaaat
     7921 gatatctatt atttggtttt t
//
LOCUS       DAZKSR010000116         7890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000116 DAZKSR010000000
VERSION     DAZKSR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.118"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..1492)
                     /gene="tyrP"
                     /locus_tag="ACG8XG_002583"
     CDS             complement(71..1492)
                     /gene="tyrP"
                     /locus_tag="ACG8XG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0774466.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="ACG8XG_002584"
     CDS             complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="ACG8XG_002584"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0774467.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3903..5159)
                     /gene="tyrS"
                     /locus_tag="ACG8XG_002585"
     CDS             complement(3903..5159)
                     /gene="tyrS"
                     /locus_tag="ACG8XG_002585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0774468.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5271..5476)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5739..7040
                     /locus_tag="ACG8XG_002586"
     CDS             5739..7040
                     /locus_tag="ACG8XG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0774469.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7109..7708)
                     /locus_tag="ACG8XG_002587"
     CDS             complement(7109..7708)
                     /locus_tag="ACG8XG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0774470.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2360 a         1600 c         1375 g         2555 t
ORIGIN      
        1 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
       61 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
      121 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
      181 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
      241 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
      301 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
      361 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
      421 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
      481 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
      541 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
      601 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
      661 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
      721 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
      781 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
      841 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
      901 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
      961 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
     1021 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
     1081 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
     1141 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
     1201 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
     1261 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
     1321 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
     1381 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
     1441 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
     1501 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
     1561 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
     1621 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
     1681 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
     1741 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
     1801 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
     1861 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
     1921 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
     1981 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
     2041 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
     2101 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
     2161 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
     2221 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
     2281 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
     2341 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
     2401 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
     2461 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
     2521 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
     2581 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
     2641 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
     2701 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
     2761 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
     2821 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
     2881 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
     2941 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
     3001 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
     3061 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
     3121 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
     3181 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
     3241 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
     3301 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
     3361 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
     3421 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
     3481 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
     3541 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
     3601 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
     3661 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
     3721 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
     3781 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
     3841 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
     3901 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
     3961 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
     4021 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
     4081 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
     4141 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
     4201 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
     4261 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
     4321 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
     4381 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
     4441 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
     4501 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
     4561 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
     4621 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
     4681 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
     4741 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
     4801 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
     4861 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
     4921 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
     4981 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
     5041 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
     5101 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
     5161 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcatg tgtaattaat
     5221 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
     5281 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
     5341 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
     5401 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
     5461 cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc
     5521 aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat
     5581 tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc
     5641 aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg
     5701 ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta
     5761 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
     5821 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
     5881 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
     5941 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
     6001 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
     6061 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
     6121 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
     6181 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa
     6241 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
     6301 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
     6361 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
     6421 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
     6481 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
     6541 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
     6601 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
     6661 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
     6721 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
     6781 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
     6841 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
     6901 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
     6961 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
     7021 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
     7081 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
     7141 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
     7201 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
     7261 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
     7321 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
     7381 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
     7441 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
     7501 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
     7561 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
     7621 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
     7681 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
     7741 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
     7801 agctagcaat tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta
     7861 ctaaaataat aaaaaaaagt tttataaaaa
//
LOCUS       DAZKSR010000117         7797 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000117 DAZKSR010000000
VERSION     DAZKSR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.120"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8XG_002588"
     CDS             complement(105..629)
                     /locus_tag="ACG8XG_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0774471.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8XG_002589"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8XG_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0774472.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8XG_002590"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8XG_002590"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0774473.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8XG_002591"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8XG_002591"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0774474.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8XG_002592"
     CDS             3157..4026
                     /locus_tag="ACG8XG_002592"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0774475.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8XG_002593"
     CDS             4052..4567
                     /locus_tag="ACG8XG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0774476.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8XG_002594"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8XG_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774477.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8XG_002595"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8XG_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774478.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8XG_002596"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8XG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774479.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8XG_002597"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8XG_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0774480.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8XG_002598"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8XG_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0774481.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2232 a         1614 c         1382 g         2569 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaa
//
LOCUS       DAZKSR010000118         7695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000118 DAZKSR010000000
VERSION     DAZKSR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.121"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1091)
                     /locus_tag="ACG8XG_002599"
     CDS             complement(387..1091)
                     /locus_tag="ACG8XG_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774482.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1278..1523)
                     /locus_tag="ACG8XG_002600"
     CDS             complement(1278..1523)
                     /locus_tag="ACG8XG_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774483.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1540..2391)
                     /locus_tag="ACG8XG_002601"
     CDS             complement(1540..2391)
                     /locus_tag="ACG8XG_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0774484.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2557..3561
                     /locus_tag="ACG8XG_002602"
     CDS             2557..3561
                     /locus_tag="ACG8XG_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0774485.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(4344..4901)
                     /locus_tag="ACG8XG_002603"
     CDS             complement(4344..4901)
                     /locus_tag="ACG8XG_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774486.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(5080..5691)
                     /locus_tag="ACG8XG_002604"
     CDS             complement(5080..5691)
                     /locus_tag="ACG8XG_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774487.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(5804..6061)
                     /locus_tag="ACG8XG_002605"
     CDS             complement(5804..6061)
                     /locus_tag="ACG8XG_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774488.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(6658..6936)
                     /locus_tag="ACG8XG_002606"
     CDS             complement(6658..6936)
                     /locus_tag="ACG8XG_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774489.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(6970..7221)
                     /locus_tag="ACG8XG_002607"
     CDS             complement(6970..7221)
                     /locus_tag="ACG8XG_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774490.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         2370 a         1451 c         1247 g         2627 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccg
      121 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
      181 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      241 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      301 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      361 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      421 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      481 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      541 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      601 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      661 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      721 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      781 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      841 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      901 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      961 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
     1021 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
     1081 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1141 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1201 cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat
     1261 aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa
     1321 taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc
     1381 agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct
     1441 gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa
     1501 ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg
     1561 gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt
     1621 taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg
     1681 aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc
     1741 atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg
     1801 actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa
     1861 ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag
     1921 tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc
     1981 atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa
     2041 agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt
     2101 caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt
     2161 tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct
     2221 ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc
     2281 tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac
     2341 cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc
     2401 tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt
     2461 gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt
     2521 ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca
     2581 attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat
     2641 ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct
     2701 caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct
     2761 caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa
     2821 gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg
     2881 atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca
     2941 gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca
     3001 caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa
     3061 aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt
     3121 gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt
     3181 ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat
     3241 gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag
     3301 gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca
     3361 gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa
     3421 ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat
     3481 ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat
     3541 gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca
     3601 gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt
     3661 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     3721 gaagcaactc cttcttattt cttggagtta aacgcttctg tcccagcctc cttattccga
     3781 ataaatggtg tccaaaagct tgcatctgcc agattatctc atttcttatg acaagctaag
     3841 caaattatct ccaccaaata atttattcat atttttgact tgcgcatact aactaattct
     3901 taaattttca tacaaaattt ctcaatttcc cttaacgagt aatatttatg tacggtgatt
     3961 ctcactagta tcccaatcac tttttaagtt atcccgattt caattctact tcaaaaatag
     4021 ttgcttttat gtatgctgtt agttgattag atggtcattt gttgagctag gtcgtttatt
     4081 ttaaacaagt tgtttcttta gcttatacct atctcgtcta ccaaacgcaa gatggcaaac
     4141 acaaatgcat acgcatctgt atttgccact ttgattcgta taaagctaac aactattaac
     4201 tcctctcagc taatagttgc tctctttttt attcagattc atttttgctg atcacagcct
     4261 agtatttcaa tggttttgaa cactaaacca aattgttcca ttacaacatt cactggctga
     4321 tgaaatggat tagtagattt cgattattca aagcgatttt ctattggtgg atcgtgttcc
     4381 caactaataa gacggattcc aaacctatcg tacagatctt ctaaaccact tataaatttt
     4441 gcacctattt caggtgtttt attgcgtgga tattctattg ttacgccatt aaatggatac
     4501 caattaggat taggctctct ttttttctgt acatctccca tagaatagtt agcactttct
     4561 gggacataat atattcttcg accatcctct aattcatcta ataagatatt tcgtgatcgt
     4621 ctatcacttg ctaaatgtag acgccacgtg cgataccatg ccatgctacc tgcatctgca
     4681 gaaaatccat caaataatgc aacaattgta tcttctatca caatcctttc cccatctact
     4741 tcatattcta atctaaaatc aaactcccct cgggtaattt ctggtcttgg tggcttagga
     4801 cccaatatta ttctacttaa aaccgcattc gttcccagat gaaacacagc tataacaaca
     4861 ataatgatta atattttgtt tctatttgat aaatttctca ttattcgcac atcacctcta
     4921 cattctcaca tattagttcg taatactcct ttaatatcaa aatatctact ggtcgtaata
     4981 atattcgcta tgaattccta ctactcgtat aagaaaggcc aaatcatcag cttttacttg
     5041 ctgatgattt ggcttgtttc tatttatcgt aattgttttc taatcaccaa aattgttaac
     5101 aatcggttca gatatttccc agttgattaa ggtaattccg tattgtgtca aaacttcttg
     5161 cgcttctccg aaattaagta cagttcttat gtatccactt tctcctaata taaagtctac
     5221 ctctctttta aaaccttcct cgagatttct aagggctata tgtggtttta atgacattga
     5281 ttgtacattt tcccccatat aatattgcgc acttcctcga ctaaaataga ttcctagatc
     5341 gtccccaaaa ggtagatctt ctctaccact agctaatcta cttctccatc ttcgttctct
     5401 tttcatagaa cctgcgctga actcacaccc gttaaactca gctatgacag tatcttcaat
     5461 tatgttaatc tgtccatcta gttcgtattc aatcctaaat ggaaattctc cataggtgat
     5521 ttccggttcg ggtattttag gtgagattaa tgcaaagaaa ggaaatatta agcttggcaa
     5581 catcaaaaaa actcctccta tgatcacaat tcccgatgta atccaaaata taatttgtac
     5641 aattctcatt gatccattgt attccatttt attcccccta ttactattca tatccgccta
     5701 tctatatcga tcttccaatg cagagataat tgcttccata ccttgatttt ttactgagtt
     5761 acctatgcga gatatcattc tccactaagg tgcatcatat tgtctatcta acatgattaa
     5821 ttctccaata ggtgtatact cctccaaaaa caacatttaa tgcatctccc tcgacaacat
     5881 aagctaccat gtttgctgta tatgtgtttg agtctaattc ataagcagat aaatttactg
     5941 tcgctggatt aaatataatc gcattcgtat ttatagctac tgaatttgct gcttcagcac
     6001 caccttttga atgtccgatc attgtgattt caatagtagg atttacgtgg acagccctca
     6061 ccgtttaccc tgaccattat agaaatcgct tacttttttg aactacgcaa caaaggatac
     6121 aatgctccta acttcactgt ctccattttt gtccactaag gaatataaaa aataggcatt
     6181 aattcattta attcggtcac aacacgttac cttttaaatc attatactgt atctcataca
     6241 ttattatcaa taaactatta taaaaatgca aaaaatgctt tatcaatcta ttttgtaatt
     6301 ggtcataata tcattgaaat cataccctgt atcgattttt cctatgaatc tcgtctattt
     6361 gtgattgacg aaaaagaggc aagacagaca ctggaatggt aattttaccc actattttgc
     6421 tccaactatt tgtaatcgta cccacttttg aaataccgag atgatcattt taattgatat
     6481 gctgattccc aagcaaaacc ccaagaaatt aaatgctcta tcgcatctag cttcttgggt
     6541 tttgcttata taaattccaa catttaaatt ttttatgctg ttcggtctca gaaatgccgt
     6601 ttttgcttat atcacaatat caagatgtca aattattcgt gttgccgatg actttgatta
     6661 atttttttca aattgtttat taagactaat gctgtctttt tgcagatacc aatgttcgaa
     6721 ctttttaaca ttatcatatg atcctgcaag ttttaacttc ttaccattag atgttatcaa
     6781 agttataatt aaccttgcct ttttcccatt aggatctttt attttatgaa ttttctgcag
     6841 gtaatctctc ctaatggcag ctatttttac ctcatcagtg ctgataaacc attgatcatc
     6901 cacatacata ctgcctgtta tgttgtgttt ttccatttct tccgagaacg aaaagtttaa
     6961 ggctcgttct tacttccaaa gttgaatgag tccatggatt ggacttagtg tgagaattga
     7021 gaacaacaat aagaatccat atcctcccgc aaacatccct aaggctatac tgtctctcca
     7081 tccataagta ggattagaag agaaaagagt caccgtttgt aataatatga aaaggaataa
     7141 tccagcacaa acaccaaaga taacaactcc aaacattgaa tttttataca atgaccattt
     7201 atttataggc ttttctttca ttttacttcc ccctactgtt ctgttcacac ggaatgaatt
     7261 caaagttcat aagccctcct ctttgaacat atgaatacga cttgctaact atactttaaa
     7321 aaagcttgtt ttaagcggtt ataggcgagt tgtaatctct attcatctca ttagcaaata
     7381 acttgccgaa aagtctactg aaattaaagc agatagtgga agaattattt agccaggata
     7441 gacactattt gtgaaacaca tgtctttact tttgaaattc tatgatgaaa agccttggaa
     7501 tacagtagct cttaattggt aatacttttt ccattataaa taacctcttt tcattttata
     7561 tttatcccct ctaaaaataa tatctaatct aatattttaa acttatattt ccctcgctgt
     7621 gctaatagag cattactaaa taaatagaaa ctagaaaaaa gcattacgcc tgccagaata
     7681 aaccaattat ttgta
//
LOCUS       DAZKSR010000119         7687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000119 DAZKSR010000000
VERSION     DAZKSR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.122"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..645)
                     /locus_tag="ACG8XG_002608"
     CDS             complement(<1..645)
                     /locus_tag="ACG8XG_002608"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0774491.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFK"
     gene            complement(645..1781)
                     /locus_tag="ACG8XG_002609"
     CDS             complement(645..1781)
                     /locus_tag="ACG8XG_002609"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0774492.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1813..3264)
                     /locus_tag="ACG8XG_002610"
     CDS             complement(1813..3264)
                     /locus_tag="ACG8XG_002610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0774493.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3308..4072)
                     /locus_tag="ACG8XG_002611"
     CDS             complement(3308..4072)
                     /locus_tag="ACG8XG_002611"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0774494.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4100..4798)
                     /locus_tag="ACG8XG_002612"
     CDS             complement(4100..4798)
                     /locus_tag="ACG8XG_002612"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0774495.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4810..5589)
                     /locus_tag="ACG8XG_002613"
     CDS             complement(4810..5589)
                     /locus_tag="ACG8XG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0774496.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5605..6549)
                     /locus_tag="ACG8XG_002614"
     CDS             complement(5605..6549)
                     /locus_tag="ACG8XG_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0774497.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6595..7374)
                     /locus_tag="ACG8XG_002615"
     CDS             complement(6595..7374)
                     /locus_tag="ACG8XG_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774498.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2174 a         1617 c         1174 g         2722 t
ORIGIN      
        1 tttaaaaatt tcaatcaaaa aacgatgatt tttagaatat gaaattcttc caatatgtcc
       61 cattacaaac gtctgacttg aaattcctaa tttcattcgt acatcatttc taatactttc
      121 atcataataa aattttttag catccactgc atttggaatc aaagtgaatt tatcatttcg
      181 aaacaaaaaa tcacctgcta cttttccaca tgaaaattca tgtgtcaaaa cagacgttaa
      241 cttacatcga taatattgct ttaaaagata tttaaaatct ctatctatcg atgtattatg
      301 gctatgtgct atccgtatcg gaatcccagc tttttttgca gcttttaaag gtaaataact
      361 catcgcatca atatgagaat gaacaatctc atattctgga tgatccttaa aaaattgttc
      421 catccattta aaatatttag gataattttg tggataaagt ctcggtgcat aatagacctt
      481 tcctccaaga cttattattt cctcatcata atcactttta tatggacgat gtgtcaaaaa
      541 atcaaactga attttagttc gatctatatt cctataatag ttcattagca tcgtctccaa
      601 ccctgcacga tgcatatcat tcacacactg taatattcga atcattatat atttccccta
      661 tacaaattta atgtttcttg tacgatttta tcccaattat atttactaca aataaactca
      721 gtagcttgat tcttcaaaac tttaaccatt tccgattgat tgcatgcctc ttctaatctt
      781 atctttaaat cacttacatc cgatttctta aaaatcatag ccttgtcttc tactacttcc
      841 gtacactctg agatatttga aactatacaa cagttcccat aactcattgc ttctaacaaa
      901 cttaatggca ttccttctaa atcacttgga agtgtataga tatacgcatt actatataat
      961 tcttctaatt cctgtccttg tacaaatcca gtaaatataa ttcgctcatc accttttgct
     1021 aattccttta gttcatttgc aaattcatct gtatcagagc ttcctccggc tattataagt
     1081 tttttatctg tctgaacatc tttaaatgcc tcaattagat accgaattcc tttttcggga
     1141 accaatcgcc ccaaaaacaa aatataagaa tcttttgtta atccatactt ttctgtaatc
     1201 aatcctgctg attttatttc aattttttta acgccatttg gtataaacct tgtttttctt
     1261 ccatatatat cttcaaaata attttggacg ctttcactaa gtacaattat ttcatctgca
     1321 aattttacag cacacttttc ccctaacatg atgtatgtac tagcaagctt gttccatttt
     1381 tctctttgat gatctagacc atgaattgtt gcaacacatc ttttcccaaa aagttttggc
     1441 aaccacaaca tagcacatgg tccttctgca tgaaaatgaa caatatcata ttttccgaat
     1501 gctgctctaa ttccaccaaa tactgaagaa ctcattgctg caattccttt tatatttaat
     1561 gtaaaaatag tctttaattt tacaccttta tattctttat ttttatgctg gtcaaattcc
     1621 tttccgctaa catgatgacc acttcgatta tagcaagtca cttcatgccc aagtgcaacc
     1681 attcgcgttg ccaattcttc taccactatc tcaattccac cttctcttga tggaattctt
     1741 ttatgaccta gcatacagat ttttaatttt tttcttgcca tcacgtttta ctccttctat
     1801 agtatcttta tcttacatgg aaccgtttct atgaagcaca gttttgactg tcctaaacaa
     1861 aattctaaaa tctaatccca tactccaatt cctgatatat tctgcatcaa gttttacaac
     1921 ctcttcgaag tctgtgatat cacttctacc actcacctgc cacattccag taatcccagg
     1981 tttcatcgca attctcgcac gatgatgaaa atcatacttc tcatattctg acttcaagca
     2041 aggtctaaac cccaccaggc tcatttttcc cattaggaca ttataaaatt gtggaaactc
     2101 atccaatgat gtagatcgaa taaattgtcc aattcctgtc ttctttgttc catcaggaag
     2161 tattttattg ccaatgattc ttgggtcaaa atccatctta aacatgagat tattactcat
     2221 cttattttct ttcatcaact ctgctttgcg ttcttctgca tccatataca tactacggaa
     2281 cttatacatc ttaaatgtct taccattttg accaatacgt tcttgtgcaa agaaaatcgg
     2341 ccctggtgat ttgatgtaaa taactggtgc tacgaagata aaaataacac ctgttgcaat
     2401 acatccaata attccagcaa gaatgtcaat cgttctcttt ataaatgctt gcctcatcgt
     2461 cataacatta atactagatg taataacagt ataatcacca actcggccaa taaattgttt
     2521 tccacgtgta ctatcagaca cttttgtaag attcatatgt acagcaagtc ccatttccga
     2581 acatctggcg attaattctt tgggatatgg aacactttcg tcaacattga tgaatacttc
     2641 atctacccaa ttctgtaaaa gatattccgc tgcattatca gcatttgcaa ctactgggac
     2701 acctccgatg gtagttccat acatatctct gtctactaca atcacaccac taagtttata
     2761 catgccataa ttcttctctt taagattacg aactacattt tttgcaatct tttgagatgt
     2821 tacaataact aacgatacat cttcaccgtt tttcatgcgg tgatgcaaca accctttcca
     2881 aagtatacga attatatatg ttaataatcc atacaccacc cccattataa ataaagaaac
     2941 tctggaataa tcatttccat cctgcattgt aataaggtat aaactgcaca caagtaacag
     3001 cataaaagat tgctttatag aagctacagc ctctttataa tatcctcttt ttaaaacatt
     3061 tttatatgcc tcaaataaaa atataatcgt aagattctca agttctataa aaattgccat
     3121 gtggcgataa ataggaatca catacggatt ccagtcaccc tgtcgcatta catatgatat
     3181 ataaaaagca agctggacac aaaataagtc taataaaata aaatcaatat gtttagacca
     3241 accacttgag ttttttctat acatctctca ctctctcctc atttcctttt tatctacgaa
     3301 taaagaacta aaataaacca aacttcttct tttttacctc ttcatagatt ggataattga
     3361 tctcatcccc gttgatcaga tcctttgcga cttgtttcat ctgtactact ttttccttac
     3421 caaactcctt ggcaatctgc tcatagcact cttttaagta aaaggtgcgc ttagagaccc
     3481 cgtgtgcatc agaagcagcc atctgcacga gattatgttt gaccattgtt ttggctgttt
     3541 tctggatatc tttgccaaat acaccaacat aacttggcgc agtaagttgg gccaaacatc
     3601 ccatatctag gaacggaagc aaatgatttg ggtcttctcg aaaatgcgcg ttgcgttctg
     3661 gatggacgat cactggtatc tttcctagtt ctaacaactc aaaaaaaagc cgttctgcat
     3721 ataacggcac ttccagtgtc ggaaattcga tcagaagata tgtatcatct aaatctgtaa
     3781 ataaaatttc atctcggtgg atgtcttcga tgagcgttcc ggtaatacgt acttcttgtc
     3841 cttccaataa cgtcaaaggc aactgtcttt tctctaattc ggcttgtaac gaagcgacta
     3901 gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtggtgt ggtgtgcata
     3961 aaatatgcgt aatgccttgc tgaatcgctt tttcagccat tgcaatactt gcttctaagt
     4021 tttctgcccc gtcatcgatt cctggtagga tatggcaatg tagatcgatc atttctcttg
     4081 tctcctctct tcttattcct taatttccgt agtaataacc agcatctttg ctgtgttctg
     4141 ctccgttgta tacgacacct aagactcgtg cttgtaccat atctaacagt tcttttgctt
     4201 tagtcaatga ttcttttcgt gacgtatttt cccgtacgac caataaggtg ccatccgttt
     4261 ttgaagacat gatctgtgca tctgttacgg caacgactgg cggcatgtcg aagatcacta
     4321 catcaaatag ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa cttagtagtt
     4381 ctgatggatt tggtggttta ggtccacttg gcagaatcga caaattgtcg actggtgttc
     4441 gttggatctc atctgctaca cttcctgatg aactaagaac agtacttaat ccgcttgcgt
     4501 tgttcaactt gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg actagcagca
     4561 cccgttgacc agatttggca aaaacaacgg caatgtttgc tgcagtggtc gatttccctt
     4621 ctccaggtcc tgatgaagtc acaacaatcg ttttgatttg ctgaccagct gcggaggcaa
     4681 actggatatt cgtccgaatc gtacgatatt gttcagcgat tggtgaagag ggattggtga
     4741 gtgtgaccaa actgactgca tgagtttggg ctgctttttg tttctgtgtt cgtgccattt
     4801 tctttcctcc tagactcttg agcggcttct gcgtgatggc gctgttttat cgtttgtagc
     4861 ttttggttgt gtctgagctg gtcctttttt acggatacca gcattcaatt ctttcgctga
     4921 catttctgga actgttccta agattgggaa tcctaatgta tctgtcacat atttttcatc
     4981 tttcactgtc cgatcgagta attccaataa gaatgccaga cctacaccta ccatcattcc
     5041 taacattaag ccagtcatta aattcaattt attatttggc gaaacaggtg tcgtattggc
     5101 aactgcacct gaaatgatcg agattttatc aacattcatc acatcttttg cgttctcttg
     5161 gaaaacttgt gaagttgtgt tagcgatctg ttgagcagcc actgcattcg tacttattgc
     5221 ctgaatcgag aacatttgtg aattttctgc ctgattaact gagatggcat ctttgatttg
     5281 ttcagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt cattgagcac
     5341 taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg tattgacatc
     5401 attgacattt gcagtgtctg attgtggcag tgtcacgatc aattgtgctt gtgaactgta
     5461 ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag caaacattga
     5521 gatgatgatt attaccaagt gtttgcgtaa tgtttcaaaa atttctttca agctgattgt
     5581 ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat cttttaattc
     5641 ttttttcaca cgatcaagtt cttgatcact gactttttga taggagatat tatcttgcat
     5701 gaagccttct ccttgcagct gatcttgctt aatcgtacca aatgcctccc gataatctaa
     5761 agcaattttt ttcatttcat caaagttcat gtctgttttc atattcgact ctagagcatt
     5821 caaaacggat tgatagttgc tgacaccgtt tagactcaat acttttttcg ctacgccttc
     5881 tacgatcttt ctttggcgcc cttgtctacc gtaatcacca tttgggtctt cataacgcat
     5941 acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg gaaagtcata
     6001 gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg catcaacgag
     6061 ttcctttagc cctttcatat tgatagagac atagtgatcg attgggatgt ctacatattt
     6121 ttgtacggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt tgatcttgtc
     6181 ttttgtgtca cgacctacga tgtcgacata cgtatcacga ggaatgctga cgatcgttgt
     6241 ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc cttgatcgac
     6301 ccgacctaaa tctccagtat caatccccat caatagaaca gagaaagatt cttgtttgtc
     6361 cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt aagttttctc
     6421 aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg ctaatactaa
     6481 tacgataaga actgctaaaa taatcagaaa tatttttttc cctcttgatt ttcttcttcg
     6541 tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta actgtcaata
     6601 gcttatattg ctatagccca tttgctcagc aacagtttgt gcttttgttg cgtatgctgc
     6661 gcttattttt tcattccgga tctgctctat ttgactgagc accgtcaaat attgatcgta
     6721 agtagcggtg gcagtcactt gaccatcctt taacatcgta tcgaataact gatctagttg
     6781 cgtgacttgc gcgatctgaa tatcagcgaa tcctagatat tgcataacaa ccgttttcca
     6841 aggactatct ttaaaaaagc tcatattttc acgtacacga gcgacatctg cacttgtcac
     6901 cttttctttg atgatgacat cgtctacagc ttgctgccag tttgatacat ttttagtaaa
     6961 taaatcgttg acagctgtct ggatcttcag ttttgattct gcactatcta actgtgtcac
     7021 gattgctttc ttctcatctt ggattgcaac agcttcttta ggcaattctg attctttgat
     7081 gtcgtaatca tctgctgaaa gtttgacaga ggatagctta tttgacaaac ttgtgattgt
     7141 ttcttctgtt atttcagggt ttagaaaaat tttttctttt gtactataca gcttgtctac
     7201 ttcttttgat aaagaagtca aggtgtcttt ttgattggtg atggcagatt ctgcataatt
     7261 gatccgttgc tgatgttgcc gctgattatt gatatggtat ccaaacaaac agatcgcaat
     7321 agcacctgca cctaccgcca cagcagtcaa tgacttgacg aatggctttt ccatagattc
     7381 actcctaagt attgatgata gataaattaa gatataattc acttacataa aaatgacagc
     7441 tttttattta aaaaaacata attaaactaa tatattcaca gaaatatttg taaacaagtt
     7501 ttccctatat aatcatacca caaactgttt tcttgactag ggcaatttac gatttaaaag
     7561 taaataaaac attaataaga taaaaattaa tcgatttcat ttttatttcc ataattttca
     7621 taggtgtttt taaaagttac acaaaaaata gaataacaaa aaaattaaca ttgaattaat
     7681 aatagtt
//
LOCUS       DAZKSR010000120         7673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000120 DAZKSR010000000
VERSION     DAZKSR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.123"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG8XG_002616"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG8XG_002616"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0774499.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(1897..2649)
                     /locus_tag="ACG8XG_002617"
     CDS             complement(1897..2649)
                     /locus_tag="ACG8XG_002617"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0774500.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(3013..5679)
                     /locus_tag="ACG8XG_002618"
     CDS             complement(3013..5679)
                     /locus_tag="ACG8XG_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0774501.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6020..7471
                     /gene="cls"
                     /locus_tag="ACG8XG_002619"
     CDS             6020..7471
                     /gene="cls"
                     /locus_tag="ACG8XG_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0774502.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2094 a         1679 c         1364 g         2536 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca tcataaaaag aacccttcac gagtcgtgaa aggttctttt
     1921 tttatctgcg gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg
     1981 tttttcactg aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc
     2041 atagtatagc ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt
     2101 tttcttcact ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg
     2161 cgatgtatta ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc
     2221 atcgattgat tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt
     2281 ttctaattga ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt
     2341 ctcatttgat tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc
     2401 atttttttct gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc
     2461 atagcgatta atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc
     2521 tgctgtttca gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat
     2581 agttacttgc gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt
     2641 ttcattcatt tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca
     2701 ttctcctctt ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc
     2761 tatcataaat aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata
     2821 aacagtgtcc aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt
     2881 ccttaaacgg tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg
     2941 agaagctgtt tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct
     3001 cgttgattcg ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat
     3061 caaaacaatt gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc
     3121 tgatgggata gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct
     3181 acatacaact acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt
     3241 ttgtgtgttt gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa
     3301 tgacatggct acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac
     3361 agcaatccca atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat
     3421 tcctgaattt ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc
     3481 taatgcaatt gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt
     3541 aaatggattg gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt
     3601 tcctgcaaat agataagaaa tagctttctt gatattatca aacacattac gtcctacttc
     3661 tactgcgctg acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt
     3721 tgccacatct gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc
     3781 gtcatttaca ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac
     3841 gatacgaatt ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc
     3901 gtttagttga tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc
     3961 ttcaaaaatc ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat
     4021 catgattgtt ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg
     4081 tggaggatcg atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga
     4141 taaatcaaaa ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga
     4201 aaaagcttca ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc
     4261 accatttagc aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa
     4321 caagtaattt ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa
     4381 aggaagttct gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt
     4441 ttcactaaag tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact
     4501 gatcgatgca tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca
     4561 ttttgaaggg tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac
     4621 tgtcattttg ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc
     4681 aagtgtttca actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt
     4741 tcccaaagat aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc
     4801 tacggctacg gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc
     4861 tccaagataa atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc
     4921 caaacttagt tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt
     4981 tgttgattcc aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac
     5041 taagaattta ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc
     5101 tccgattggt accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc
     5161 tgtcagcata ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg
     5221 tacataatcg cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat
     5281 cgttttttcc ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc
     5341 ttctaaagaa ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa
     5401 tactgcaaaa ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac
     5461 aatcagcaat acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg
     5521 cttcttcggc ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg
     5581 cgtggtgctc aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc
     5641 tttatagacc gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga
     5701 tcttggccct agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag
     5761 gtctcaccat ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg
     5821 caacaaacgt tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt
     5881 attaacctac aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg
     5941 aaaagtatat tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta
     6001 tgtaaggagg aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc
     6061 acttattgct ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg
     6121 gctcttagta ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg
     6181 tggattaacg gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa
     6241 ctttcagtct tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga
     6301 caaacagcaa tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa
     6361 ttctgtcact cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa
     6421 aaaagccaaa cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca
     6481 gcaagtctta catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata
     6541 tgatgcattt ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg
     6601 tggacatgtc catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta
     6661 ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa
     6721 tattgccaat caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat
     6781 acggattttc ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt
     6841 ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga
     6901 tcgagatgaa tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa
     6961 cgaaagggaa caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt
     7021 ttggatacag acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc
     7081 aactgcctct ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat
     7141 ttatcgagca acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt
     7201 ctatgagaac ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt
     7261 aggttcagca aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat
     7321 ttacgatcct gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg
     7381 ttcatccatg acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca
     7441 aatttctcga ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac
     7501 taagattaaa gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg
     7561 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
     7621 ttgaacttgt tccttgtgac tcggagctgg acggcaggct cacgacctct ttc
//
LOCUS       DAZKSR010000121         7590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000121 DAZKSR010000000
VERSION     DAZKSR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.124"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..745
                     /locus_tag="ACG8XG_002620"
     CDS             <1..745
                     /locus_tag="ACG8XG_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0774503.1"
                     /translation="LVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENR
                     QKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDI
                     KESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDN
                     WYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIK
                     QKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            821..1039
                     /locus_tag="ACG8XG_002621"
     CDS             821..1039
                     /locus_tag="ACG8XG_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774504.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1026..3704
                     /locus_tag="ACG8XG_002622"
     CDS             1026..3704
                     /locus_tag="ACG8XG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0774505.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3695..3892
                     /locus_tag="ACG8XG_002623"
     CDS             3695..3892
                     /locus_tag="ACG8XG_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774506.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3896..4507
                     /locus_tag="ACG8XG_002624"
     CDS             3896..4507
                     /locus_tag="ACG8XG_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0774507.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4492..5883
                     /locus_tag="ACG8XG_002625"
     CDS             4492..5883
                     /locus_tag="ACG8XG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0774508.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6099..6413
                     /locus_tag="ACG8XG_002626"
     CDS             6099..6413
                     /locus_tag="ACG8XG_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774509.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6406..6900
                     /locus_tag="ACG8XG_002627"
     CDS             6406..6900
                     /locus_tag="ACG8XG_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774510.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6968..7282
                     /locus_tag="ACG8XG_002628"
     CDS             6968..7282
                     /locus_tag="ACG8XG_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774511.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7381..>7590
                     /locus_tag="ACG8XG_002629"
     CDS             7381..>7590
                     /locus_tag="ACG8XG_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0774512.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2923 a         1023 c         1455 g         2189 t
ORIGIN      
        1 tcttgtgaaa agaaaaaatg gcttcaataa aagtaaaatg caaatgttcc caaattttta
       61 ttatttgtta aagcctgttc tgtgcaaaga tgatgtttat aaaattaata agataaccca
      121 tcaagaaaat agacaaaaaa acttgatagt tcaaaatttg aactatggaa caaatcaata
      181 ttctgttgat agttcaaaat ttgaactatg cgacacatca atgaaaacaa gagacaaaaa
      241 caattttgct gatagttcaa aaaatgaact agaattatat aagtcttata aagatagtaa
      301 agatattaaa gaatcacata atgatgatct tcttgaatca ctttcaacta aaaataataa
      361 attagatgaa gaattgatcg aaacatttat tgaagaaaat cttcttaaac aaatatgggg
      421 agaaagtatt gtgaattgct taaaactggc tagcaatagt gattatcgtc aattcgacaa
      481 ctggtacaaa aaattcaaat atgcgattag atctgctgaa aaagaacaaa aaattcaact
      541 gaaaattatc tatgaaattt gtaataacaa gtattttgta gatcatgtta gagaacagct
      601 atcaatgact ttacgtgact tgatacgaag agcaaaaaca gatcatcgta tcaaacaaaa
      661 agacgattat atctttgctt ctctaaaaaa taaagcgtta gaactaattg aattgcaaat
      721 cagcgaagga atagataagc aataagaata gaattgagtc aaatgaaatg gaggggtatc
      781 tccacttcat ttgagaactt tgaatatagg aggggttatt ttgattaaaa atcgtttaat
      841 agatcaagtt tggtgtctag atcaaaatac agatagtctc gaattaatcg agactatttg
      901 cttcaacact atagttctcg atttaagagc tgaaaatgat aattgtgtaa ctcatgtcat
      961 aattaatcaa aaaatggctc agaagctctc tgagagcctc agaaaatggg ctaagggagg
     1021 taacgatgaa aataattgaa aaaaagattg ccaataaaaa aataaattgg ttggttgtta
     1081 attttcttgt tctgatagct atgattattt ttacaagtaa tattgctcat gcgagtattt
     1141 tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac
     1201 cttacctaaa aaagtacaat ggagatttcc ttagtcagtt cgggatttgg ctcggatggg
     1261 ctgtggtaaa aggcatgttt actgtgacag attcgattca aaatatgatt ccagatgtgc
     1321 tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagccgtaa
     1381 atacaattgt cattggatta atgatccttt cattgatgtt tgttggttac aaaatgatta
     1441 ctggaaaagg aacagtggat ttaaaatcgg ttggaatgaa cgtagtgatg tctatagcat
     1501 taatactttt aatgccaacc atgatttcta gtggtattca atttgctaaa acattttata
     1561 aggactctac tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag
     1621 gagtaacaga tttagcatat attaataaaa cagatcagta ctcaagcatt gataaaacgg
     1681 aggatcgaaa taatttaaca cgaaaaaatt ttcaacagac ggatctcaca caagtgttaa
     1741 cggataaagt gattgataaa ttggaaaaag aaaatccaat tgcggacaat cttcgttatg
     1801 aattagttga aaattctaat aatgaatttg ttgctacaaa gttttctgac aacttcttat
     1861 caacattttc tgatagcttg aaatcaggct attaccgata ccaagcaaat ctatggggga
     1921 tttcaattgg gttgacagcg ttagcaatcg cctatgtgtt cagcgcgttt gtgatcatta
     1981 cagcaatact agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcaacagata
     2041 ttgagacagg acagcgttca aaagttgttc tatcagatat tctacaatgt tacttaactg
     2101 ttgggtttca aggatttggg ttatcaatgt ttgctatgtt tatcaatttt ctgaactcag
     2161 gacaaggtat ctcaacaaat atttttataa aaacaattgc ttatatctgt gccgtttttg
     2221 ttttaatcaa aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag
     2281 aaggctatgg gcaacttgct tctgcttttg gaatgggcgc aatgctgttt agaaaaggat
     2341 caaatggatt taatcgtgct aaaggaagcg gagaaggaga agatcgaaaa ccagaaaaga
     2401 atttcggcga aactctatca aaaaaagctg gcaaaactgg aagagctttg ggatatgccc
     2461 atgaacgtgg attatccggt ttagcttcag atggagctac aatggctagt gaaagagcaa
     2521 caaaaccatt taagagtatg cgtgatatag ctaatgatac caagaataag tttaaagaag
     2581 gtttagatga tggtacagtg tccgctatta ataaaaatag caaacctatg ttagctaaaa
     2641 ataaagaaga tgaaactgga aaatatgctg attccgtgcc aacacgtctt tcagatagaa
     2701 aagatggtgc taaagttgaa aatgccgata gaattatgtc tagttctgaa cgtatgcgtg
     2761 aagcaatgaa aaataatgct gaatcgaata ataatcctgc aagcgcaatt cagcaaaaag
     2821 tccaacaaga tattgaagaa cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag
     2881 aattaattaa tcagaagcgt catgaagcga aatacacgcc tgaagcaatg aatcgcgagg
     2941 aaataatgtt aaggaaacgt gtagaagggc ggacaggcgc taatattgac ggaaaagaag
     3001 cgttgaccaa agaaagaatt caagaagcta aaaattcaaa tacaggatta gaaaaattag
     3061 tcaaagaaaa tccacaaaat tctacaagta gtcatggtgg ccgatcagta gatgtccgag
     3121 aaaatattca aggttcactg aatggtcatg ttggacggaa tgttgatgta agagagaatt
     3181 tacaaagttc ttcaaatgct caaggtacta agacaattga tgttcgagaa aatctgcaaa
     3241 gtagcagtac aagtcaacca aaaacagtag atgttcgaga aaatgtacaa aattctacaa
     3301 gtagtcaagg gtttaaatca gtggatgtcc gagagaatgt gcaaggtagc agtgcaagtc
     3361 aaccaaaaac agtagatgtc cgagaaaatg tacaaagttc tacaagtagt caaggattca
     3421 aaacagtaga tgttcgggag aacattcaaa aagatagtgg agaaatgaaa gaaaaaacac
     3481 aaaaaatcaa tatcgttgaa gacagaaagt cagcgaaaaa atttgattca aatcatgaaa
     3541 cagtgattat tgatagtgaa atccgtgaaa atgataaggg atcaaaatca agacgaagat
     3601 tcacttatga agacaatgag ttattcagag atacacttaa tgatcctaat ccacttttcg
     3661 ataatttatt aagaaataaa agggggggat tgtcatgcaa ttaattctaa agattagtac
     3721 gcatgatttc ttagaacttg acgaattatg tgaatcagta aagaacgcaa caaatgaaaa
     3781 aatcacggtc actaattata ttgaactgat gattcaagaa gaattaaaga tgaaaaatga
     3841 aatagagagc aatttcaaat gttgctctct agaaaaaaat gtgaggggat aatcaatgga
     3901 tgataaacaa aaaatgtacg aaaaaaatcg caaacatagt gaggccttaa attttcgtgt
     3961 cgataaaaag acttatcttg aactgacatt gattgcaaaa caaaggggaa tttctagagc
     4021 agacgtttgc cgtgagcggt taaagggact caaatatgaa ccgccgttgt tagctgaaga
     4081 tgatagggta gttttaacca aacagttaaa tcaaattggt aacaatttga atcaagcggt
     4141 aaaggcatta aatatcattg ctaaatattt tagagaagaa tctgaagata aaaaagaaaa
     4201 gagcctacat tttagttttg atgctagccg ttacaacatt tttaaagatg aacttgcgaa
     4261 tagcatagca aaaaagttga tgattagtta tccagatgac atttttttgg agtccaatcg
     4321 gagtgccccc tcttttttta aattatcaga agtagaaaaa gaatatttcg aatattggat
     4381 agaaaagcaa actagaccgg gtacgaaaga agagtattcc caaaaagttg atcggaattt
     4441 aattcactca attcagatta ttgcaagttt aaaagatgag gtgaaacaac tttgggagct
     4501 actataacat cgaaaggacg caagaaaaat ttatcctcgc tattacaata tgcagctaat
     4561 ccttcaaaaa aagaaaaatg tgttgcagtc tcaggaataa attgtagtga tgatagtgag
     4621 gaagcactgc aagaaatgaa tttagtacgt accaagatga ataaatctaa agatatagca
     4681 caagcgtttc atgttattca ttcgtttgat aagagtactt ctaaagagtt aagtattgat
     4741 aaaatgcacg aaatagctgt cgaatttgca gagaaagctt ttccaaattc gcaaatcatt
     4801 gttgcaagtc acaatgataa agatcatttt catagtcatc ttgtcattaa taatattaac
     4861 atggaaacat gtaaaaggtt gcgtattgac cctaaagatt tagaactaat gcgaaaaatt
     4921 aacgatcaag tcctcaatga acatggtctt gaatcagtga ataatgagta ctatgaaaac
     4981 ttagaaaaga gaaccaatct ttattcaaac gatccttcta agatgaaata tggaaaaatt
     5041 aattatcaag agtttgtgca aaatgcgatc gaagcaacgt tggaaaaatg cgaagtgaat
     5101 tcttttgaag atttttcgga tcatttggca aaagaatata acattgaagt ttatcaattt
     5161 tcaaaaacaa ataacaaact tggctatgtt ctttacaaac aaggtgtaaa tttctatgag
     5221 cgtagaggtg agttaattac tttaggaaga gataaacaat ctagaaaaga aaaaagtcag
     5281 tatgtcgaac gtacttttag tgcaagaaag ttagggaaaa gatattcatt tgaatctgtt
     5341 tctgaacaac ttgaaaagaa tttagaagaa tcacatataa aaaatttttt taatatggaa
     5401 agagagcaag ttatgtcgct aattagtgga gtaaattaca aaactggtga gttaaatgta
     5461 ccgtttgtgg aattagattc catcgcacaa aagcaattta gcgaccgtga aggcgatgaa
     5521 aaattcatta aatatgatga cttcttgcga gaggtagaca agcattttgt aaatacaatg
     5581 tctttagaag atgatcaaga atacacgatt aaacttcata cggatgtgaa tcctcaaact
     5641 ttacgtagtg cgaaaagtaa aacaatttct attaaaaaga ctgatcagaa agagttattt
     5701 ctaccggcaa agggttttga aaattgttct catacacctt ctgctaaaca gattcactat
     5761 caaatcagtc gcataatgaa tgacgatgta aaaatgtatc gtgctaaaaa ggattatgaa
     5821 ttcacacaac aaatgattga aaatcagcaa aaaaggcaac aaaattttga aatggaattc
     5881 tgaatgggct tgttagtgga ctttccagtt ttttgcctat gaagagttgc ttggaaagaa
     5941 gcagttttta ggacatacgc cagctaccca tctacttgcc agtcaatccg ccccgctcac
     6001 gctaggctac tttctggcaa gtcagatggc gggtaaatct ctgagggagc aagctcccta
     6061 cagactccct cccaaaaaaa gaaaggtgga agaaaaaaat gaactatcca tattttaaag
     6121 tctcggcttc agaagaaact aaagaaatat tcaacaattt ttacaaccaa aacaaaggaa
     6181 tttttggttc aaaggcaaat atgtttcgtg ttatggtttc aaatttacca gtgttagcta
     6241 gtccatcaaa taataaattt aatgatcctg aaagcattaa atttgaacaa aaaatttcag
     6301 aactagaatc aatgataagt aatgaagtaa ttgaaaagct ggatgacatt gatcaaaagt
     6361 tatcgtattc tctaaaaaac aaatacaaaa cggaggagaa aaaagatgtt taaagtttac
     6421 ttcccgtttc agtggctcgt taaaggaatt cttttagtgc tactaggatt gattcttttt
     6481 ggaagttatc taacaattaa agccagacat tttccagaag aaaagccaat aaaaattgta
     6541 acaaaagatt taaaaatagg tgaaacttat caggcagatg gggttgaact aacatttaaa
     6601 aatattgaag tgaccgaaaa aagaaatgaa tttgcacaac atgatcgtgt attacaagtg
     6661 acctatacac ttataaatga tacagaaaat ccgatacgtc cacaacaatg gtcgattgaa
     6721 aataaggatg gtgaagaact ttatgagtat gatatgatat tggataatcc agagtatatt
     6781 gaaagtggca aagaagaaac agtaaattac tattatgaat taccgattga aaggcaggac
     6841 taccaaatcg tagtacgtag agagaatggt tacatagttg ggttgccatt tactttataa
     6901 gttttatttt gttagtagtt tttaaggtaa tatcagtttt gtgatttttg aaaatggaac
     6961 tgggagaatg gctgaggaaa ataaagttgt tccgtttgaa acattagtga taaataagga
     7021 agagtttgag caactttttc ttgatttttt tattcaatat gacatgattt atgattttag
     7081 ttcgtcacta gatttatttg aattagaagc agaaataata gaaaacagtc gagagaagaa
     7141 tggtaaaatc attatttcag tagaaaaatt caatgaatta ttctcacaaa catttgaaat
     7201 agatccagac tattatcaac cacaagaagt ttttcgagag tttagaaata agattattgg
     7261 ttttagtcaa actacaaaat gaaagcttaa taaagcaagt gttgatagaa ttattcctca
     7321 acgcttgctt tattttatgg gcacttcata atagaggtat agagaagaga ggtattcatt
     7381 ttggatacta ttactccaac aaacttcaaa gaaaatatag gtaaagtgtt gcaagaagta
     7441 gttcattcaa attctgaaat agaaattact agggattcta aaaatggatt gaacgatggc
     7501 gtcattatga tttctaagag aaaatgggag ttgttacaag aagaacttta tttacaacga
     7561 acgggtacat gggcttatgt atctgattta
//
LOCUS       DAZKSR010000122         7474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000122 DAZKSR010000000
VERSION     DAZKSR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.125"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG8XG_002630"
     CDS             275..1753
                     /locus_tag="ACG8XG_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774513.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="ACG8XG_002631"
     CDS             2030..4090
                     /locus_tag="ACG8XG_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0774514.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="ACG8XG_002632"
     CDS             4173..4742
                     /locus_tag="ACG8XG_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774515.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="ACG8XG_002633"
     CDS             complement(4917..5723)
                     /locus_tag="ACG8XG_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774516.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="ACG8XG_002634"
     CDS             5861..7375
                     /locus_tag="ACG8XG_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0774517.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2683 a         1226 c         1459 g         2106 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaag
//
LOCUS       DAZKSR010000123         7408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000123 DAZKSR010000000
VERSION     DAZKSR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.126"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..348)
                     /locus_tag="ACG8XG_002635"
     CDS             complement(13..348)
                     /locus_tag="ACG8XG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0774518.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..595)
                     /locus_tag="ACG8XG_002636"
     CDS             complement(332..595)
                     /locus_tag="ACG8XG_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0774519.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="ACG8XG_002637"
     CDS             complement(1055..1858)
                     /locus_tag="ACG8XG_002637"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0774520.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="ACG8XG_002638"
     CDS             complement(1836..2960)
                     /locus_tag="ACG8XG_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774521.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="ACG8XG_002639"
     CDS             complement(2976..3791)
                     /locus_tag="ACG8XG_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0774522.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4653)
                     /locus_tag="ACG8XG_002640"
     CDS             complement(3781..4653)
                     /locus_tag="ACG8XG_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0774523.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="ACG8XG_002641"
     CDS             complement(4738..6027)
                     /locus_tag="ACG8XG_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0774524.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6171..6683)
                     /locus_tag="ACG8XG_002642"
     CDS             complement(6171..6683)
                     /locus_tag="ACG8XG_002642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774525.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(6750..6893)
                     /locus_tag="ACG8XG_002643"
     CDS             complement(6750..6893)
                     /locus_tag="ACG8XG_002643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0774526.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2278 a         1589 c         1136 g         2405 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DAZKSR010000124         7227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000124 DAZKSR010000000
VERSION     DAZKSR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.127"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8XG_002644"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8XG_002644"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0774527.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8XG_002645"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8XG_002645"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0774528.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8XG_002646"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8XG_002646"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0774529.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8XG_002647"
     CDS             2927..3532
                     /locus_tag="ACG8XG_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0774530.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8XG_002648"
     CDS             3857..4180
                     /locus_tag="ACG8XG_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0774531.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8XG_002649"
     CDS             4362..5732
                     /locus_tag="ACG8XG_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0774532.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8XG_002650"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8XG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0774533.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
BASE COUNT         2064 a         1450 c         1313 g         2400 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgt
//
LOCUS       DAZKSR010000125         6952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000125 DAZKSR010000000
VERSION     DAZKSR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.128"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..1035)
                     /locus_tag="ACG8XG_002651"
                     /pseudo
     CDS             complement(97..1035)
                     /locus_tag="ACG8XG_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(1035..1652)
                     /locus_tag="ACG8XG_002652"
     CDS             complement(1035..1652)
                     /locus_tag="ACG8XG_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774534.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(1666..1836)
                     /locus_tag="ACG8XG_002653"
     CDS             complement(1666..1836)
                     /locus_tag="ACG8XG_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774535.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(2184..3674)
                     /locus_tag="ACG8XG_002654"
     CDS             complement(2184..3674)
                     /locus_tag="ACG8XG_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0774536.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(3717..4016)
                     /locus_tag="ACG8XG_002655"
     CDS             complement(3717..4016)
                     /locus_tag="ACG8XG_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774537.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(4382..4666)
                     /locus_tag="ACG8XG_002656"
     CDS             complement(4382..4666)
                     /locus_tag="ACG8XG_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0774538.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(5185..5670)
                     /gene="vanZ-A"
                     /locus_tag="ACG8XG_002657"
     CDS             complement(5185..5670)
                     /gene="vanZ-A"
                     /locus_tag="ACG8XG_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0774539.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5823..6734)
                     /locus_tag="ACG8XG_002658"
     CDS             complement(5823..6734)
                     /locus_tag="ACG8XG_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0774540.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2038 a         1325 c         1026 g         2563 t
ORIGIN      
        1 aattttcgat agagaatgtg ctattttttg tcaaaggtgt ataccttgac tgtgcttgct
       61 gttacattaa gtttattttt aagttattaa aaaagaaata gcttttaaag tttggctcgc
      121 tgtcgcttta taaagctgat tgacttttga ttgcaaacta cttaaagaaa acaaactcgg
      181 actattcgtt ttcttctctt tggtttgaac atcagcaatt atcccctctt gattgcctat
      241 tttagcttgt ttagaagaaa caaaagctaa aagctcctct tgggttttaa aacgctgtgt
      301 ggggcttaga acgcccttaa acgacccttg gtttactttt atactagctt ccacctcgaa
      361 aaaaggttct tttttaaaat tctctatggc ttcctggcgc tgaaaaataa ggtataaggt
      421 gggcgtttga acacgtccta gtgaaaatgt accttgtacg cccttctgtt gtaaatttaa
      481 cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg gcaatttggc gtgtttgcgc
      541 ttcttgataa aagggatagt aattcattcc aggttgcaaa ttttgaaaac cgcttcggat
      601 tacatctttt tctaagctat tgatccatag tcttttaaat gttttatctt ttgaaaaggc
      661 atttgcttta tggataatcg accaggcgat attttcacct tctctgtcgc tatctgttgc
      721 aacaataatt gtatttgcct ttttgagaag ttctgcaaca attttaaact gctttccctt
      781 atcttttgca acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt caagtttcca
      841 attttgccac ttttcgtcat aatgacctgg ttctgctaat tccactaaat gcccaaaacc
      901 aaaggtgata aacgtttcat ctgtaaatag tgggtctttg atctcaaaat aaccgtcttt
      961 tttggtgctt tgttttaaag cacttgcgta ggctaatgcc tggcttggtt tttcagctaa
     1021 aataaccgta ctcattaact atccctcttt tcattgtttt ttctttgatc gactgtcacg
     1081 ttatatcttg ctcgatacct tctaaacgtt cggcgattga ttccagtttg ttcttcaact
     1141 tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg tggatcattt
     1201 tctttaaatt tatgctgacg acctttgaat ttgccttttt tcttagcaat ttcgattcct
     1261 tgtgcctgac gttccttaat tttttttcgt tctgattctg cttgatactt gtacaattca
     1321 atgacaaggc tattaatcaa acgccttaaa ttttcatctt caataccatt cattgagggt
     1381 aaatttaaga cttccagggt tgccccctta atttgaattt gattcatcaa ttctgttaat
     1441 tctttattat ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc cccttcacga
     1501 atatagttaa gcatagcttg taattgtggg cgttcgaccg attgaccgct taatttgtct
     1561 gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt ctgttctttg
     1621 ctactgacac gtgcataacc aattttagcc attttcaacc aacctctaaa attctctcgg
     1681 ttgcaataac caatcagcaa tatctacttt ttcaatttca aattgcttat cagaaattgt
     1741 cttttcgtaa gcgataaaat cttgcgcata ttgttgctca ttaaaaatag ccaccacttc
     1801 gtcattttct aaaactcgat aaataaattt tttcatttta ctcctcctat tatgcccaac
     1861 ttaaatgacc tattcaccaa gtcaattata ctgctaaaat catattagga caaataggta
     1921 tactctaatg acctataaaa gatagaaaat taaaaaatca agtgttcgct tcgctctcac
     1981 tgcccctcga cgttttagta gcctttccct cacttcgttc agtccaagcc aactaaaagt
     2041 tttcgggcta ctctctcctt ctccccctaa taattaatta aaatcttact ctgtatattt
     2101 ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac gtatcataga tataaatgta
     2161 atggcatagt gcgggtttta ttttcagcct gtatcatagc taaacaaatc gagttgtgtg
     2221 tccgttttag ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt atgctctaag
     2281 tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt cttctttttt tactttaatg
     2341 atcgatagca acaatgattt aacactagca agttgaatgc caccatttct tcctggttta
     2401 atcttaaaga aaatttcctg attcgccttc agtaccttca gcaatttatc taatgtccgt
     2461 tcaggaatgc ctagcacttc tctaatctct tttttggtcg tcactaaata aggcttgtat
     2521 acatcgcttt tttcgctaat ataagccatt aaatcttctt tccattctga caaatgaaca
     2581 cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc cttgacggac aaataaatct
     2641 ttactggtta aatcacttga tacccaagct ttgcaaagaa tggtaatgta ttccctatta
     2701 gccccttgat agttttctga ataggcactt ctaacaattt tgattacttc tttttcttct
     2761 aagggttgat ctaatcgatt attaaactca aacatattat attcgcacgt ttcgattgaa
     2821 tagcctgaac taaagtaggc taaagagagg gtaaacatga cgttattacg ccctattaaa
     2881 cccttttctc ctgaaaattt cgtttcgtgc aataagagat taaaccaggg ttcatctact
     2941 tgttttttgc cttctgtacc gcttaaaacc gttagacttg aacgagtaaa gcccttatta
     3001 tctgtttgtt tgaaagacca atcttgccat tctttgaaag aataacggta attaggatca
     3061 aaaaattcta cattgtccgt tcttggtatg cgagcaatac caaaatgatt acacgttaga
     3121 tcaactggca aagactttcc aaaatattct cggatatttt gcgaaattat tttggctgct
     3181 ttgacagatt taaattctga ttttgaagtc acatagactg gcgtttctaa aacaaaatat
     3241 gcttgataac ctttatcaga tttgataatc atagtaggca taaaacctaa atcaatagcg
     3301 gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc aaaatcaata
     3361 aagaaggtat tgatttgtct taaattgttt tcagaatgtc ctttcgtgta tgaacggttt
     3421 tcgtctgcat acgttccata acgataaacg ttgggtgtcc aatgtgtaaa tgtatcttga
     3481 ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac gaccgccaat catgcttgat
     3541 tttgagcgat acgcaaaaat agcccctttg cttttacctg gcttggtagt gattgagcga
     3601 attttactat ttttaaattt gtactttaac aagccgtcat gaagcacagt ttctacaaca
     3661 aaagggatat tcattcagct gttctccttt cctataaatc ctataaaata ggttgtttaa
     3721 ttaacttggt ttgctttttc attcaactgt ttcaatattg catgttttga aaaagatttt
     3781 tttcctttat aagtcaattt ttttccacta atcgaataaa ttattttgtt attttctatt
     3841 aacttatata tataatcttc cccctccgaa gaaaaatact tatctgattt tgtttctaag
     3901 tagatatttc tcttttctaa ctctttctta aacgtttcta gtgtatagat atttgctaat
     3961 tttcttatct ccaataaact attttttata taagttttac attcatcatg attcatacaa
     4021 actccacctt ctataaatga atacaaaaaa agcaatcaaa cgatttccga ttgattgctt
     4081 aacaattctt aaattcagta gcttagatac ttgaaaactc tctgatttcc ctatataatg
     4141 atagtacggt tatataccgt cttcaaacaa agttaattaa ataacttctt acgagggaag
     4201 agttcatctg actaactgat aagcgttggt ttggcaatct tatcgggcta tgcatttata
     4261 aaatgtcgtc aaacatttta taaatgtgtc atggctcttt tttcgtttct attcagttcg
     4321 ttgtttcgtt atatctagta taccgctttt aaaaaaaata agcaacgatt tcgtgcatta
     4381 ttcacacgaa gtcattgctt ttttcttctt ccatttctaa atccaatgtt acttgttctg
     4441 attctgtttc tggttctggt tctgttggct catttgggat taaatccact actagcgttg
     4501 agttagttaa ctttgcaatt tgttctagtg tttttatggt tggatctgat tttcctgatt
     4561 ctattcgtga ataatttgat ctactcattt ctaattcttg ggagaatttt tcttttgatt
     4621 tggtacctct catctttttt aagctttctc taaatgctag ttccaagttt ctcccccctc
     4681 tttttgtgta ataaaattca cgctttattt aaattatagc ttttaatatc ttaataaaca
     4741 agtgattaat cacttgtgat ttgtgattat tccaaaagta ggtaatttca ccaacctttt
     4801 tcaaaagttt ttatcgacga ttattggact ggatcgtgtt tcctaaagcg attctaagcg
     4861 catgtttaat tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat tcaaaaaaag
     4921 catggctgct agtgggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta
     4981 ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt
     5041 gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt
     5101 ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag
     5161 atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac
     5221 gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg
     5281 attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt
     5341 tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa
     5401 agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt
     5461 gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa
     5521 attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga
     5581 taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc
     5641 tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt
     5701 tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt
     5761 accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat
     5821 ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct
     5881 atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta
     5941 gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt
     6001 tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca
     6061 ctatgtggta aaccaacata gcgaatatgc catggttcat attgaattcc tgttaactct
     6121 gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac
     6181 tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct
     6241 gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc
     6301 acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc
     6361 tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata
     6421 ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata
     6481 tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct
     6541 tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt
     6601 tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac
     6661 agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa
     6721 aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc
     6781 tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa
     6841 ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc
     6901 ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa tg
//
LOCUS       DAZKSR010000126         6902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000126 DAZKSR010000000
VERSION     DAZKSR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.129"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..444
                     /locus_tag="ACG8XG_002659"
     CDS             <1..444
                     /locus_tag="ACG8XG_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0774541.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="ACG8XG_002660"
     CDS             444..1643
                     /locus_tag="ACG8XG_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0774542.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="ACG8XG_002661"
     CDS             1659..2303
                     /locus_tag="ACG8XG_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774543.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="ACG8XG_002662"
     CDS             2314..3120
                     /locus_tag="ACG8XG_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774544.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="ACG8XG_002663"
     CDS             3142..3372
                     /locus_tag="ACG8XG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774545.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3886..4194
                     /locus_tag="ACG8XG_002664"
     CDS             3886..4194
                     /locus_tag="ACG8XG_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774546.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4196..4618
                     /locus_tag="ACG8XG_002665"
     CDS             4196..4618
                     /locus_tag="ACG8XG_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774547.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4638..6227
                     /gene="mobP2"
                     /locus_tag="ACG8XG_002666"
     CDS             4638..6227
                     /gene="mobP2"
                     /locus_tag="ACG8XG_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0774548.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6239..6574
                     /locus_tag="ACG8XG_002667"
     CDS             6239..6574
                     /locus_tag="ACG8XG_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774549.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6715..>6902
                     /locus_tag="ACG8XG_002668"
     CDS             6715..>6902
                     /locus_tag="ACG8XG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774550.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2648 a         1036 c         1419 g         1799 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca aaatatttaa
     3781 aaataggata gtgaaaggat agcaaaggat agtaaaagga tagaagcagg atagcgaaaa
     3841 ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata
     3901 agaaatgtag atgagaaaat cgtaagagaa attgatcgaa gatgtgacga actaagtaac
     3961 cgaacgggta ataaatggag tcgtaacgat tatttaaagc tactcgttga aaatgatttt
     4021 gatcgtcctt tgatggaata taaacaagat aaatttgatc aattattaaa tcagttaata
     4081 gataaatttt cttttgttca aagtcataat acaaaagtat tagaagaata tatttgtcag
     4141 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
     4201 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
     4261 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
     4321 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
     4381 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
     4441 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
     4501 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
     4561 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
     4621 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
     4681 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
     4741 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
     4801 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
     4861 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
     4921 tagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
     4981 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
     5041 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
     5101 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     5161 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     5221 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     5281 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     5341 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     5401 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     5461 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     5521 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     5581 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     5641 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     5701 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     5761 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     5821 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     5881 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     5941 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     6001 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     6061 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     6121 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     6181 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     6241 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     6301 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     6361 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     6421 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     6481 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     6541 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     6601 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     6661 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     6721 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     6781 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     6841 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     6901 tt
//
LOCUS       DAZKSR010000127         6885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000127 DAZKSR010000000
VERSION     DAZKSR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.130"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8XG_002669"
     CDS             complement(<1..182)
                     /locus_tag="ACG8XG_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0774551.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8XG_002670"
     CDS             517..951
                     /locus_tag="ACG8XG_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0774552.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8XG_002671"
     CDS             974..1294
                     /locus_tag="ACG8XG_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774553.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8XG_002672"
     CDS             1435..1626
                     /locus_tag="ACG8XG_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774554.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8XG_002673"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8XG_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0774555.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8XG_002674"
     CDS             2112..2375
                     /locus_tag="ACG8XG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774556.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8XG_002675"
     CDS             2399..2587
                     /locus_tag="ACG8XG_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774557.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8XG_002676"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8XG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774558.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8XG_002677"
     CDS             3070..3441
                     /locus_tag="ACG8XG_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0774559.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8XG_002678"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8XG_002678"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8XG_002679"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8XG_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774560.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8XG_002680"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8XG_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0774561.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8XG_002681"
     CDS             5250..5447
                     /locus_tag="ACG8XG_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774562.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8XG_002682"
     CDS             5553..6035
                     /locus_tag="ACG8XG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774563.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8XG_002683"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8XG_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0774564.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1058 c         1353 g         2143 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttt
//
LOCUS       DAZKSR010000128         6869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000128 DAZKSR010000000
VERSION     DAZKSR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.131"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8XG_002684"
     CDS             <1..227
                     /locus_tag="ACG8XG_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774565.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8XG_002685"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8XG_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8XG_002686"
     CDS             1635..1841
                     /locus_tag="ACG8XG_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774566.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8XG_002687"
     CDS             1862..2458
                     /locus_tag="ACG8XG_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFQ0774567.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="ACG8XG_002688"
     CDS             3410..4009
                     /locus_tag="ACG8XG_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774568.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="ACG8XG_002689"
     CDS             4058..4774
                     /locus_tag="ACG8XG_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFQ0774569.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="ACG8XG_002690"
     CDS             4788..5567
                     /locus_tag="ACG8XG_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFQ0774570.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="ACG8XG_002691"
     CDS             5650..6006
                     /locus_tag="ACG8XG_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0774571.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="ACG8XG_002692"
     CDS             5996..6262
                     /locus_tag="ACG8XG_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0774572.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="ACG8XG_002693"
     CDS             6421..6699
                     /locus_tag="ACG8XG_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774573.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6869)
                     /locus_tag="ACG8XG_002694"
     CDS             complement(6696..>6869)
                     /locus_tag="ACG8XG_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0774574.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2527 a          957 c         1333 g         2052 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DAZKSR010000129         6731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000129 DAZKSR010000000
VERSION     DAZKSR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.132"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8XG_002695"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8XG_002695"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFQ0774575.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8XG_002696"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8XG_002696"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFQ0774576.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8XG_002697"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8XG_002697"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFQ0774577.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG8XG_002698"
     CDS             complement(4028..5044)
                     /locus_tag="ACG8XG_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0774578.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACG8XG_002699"
     CDS             complement(5146..5298)
                     /locus_tag="ACG8XG_002699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774579.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="ACG8XG_002700"
     CDS             complement(5315..6634)
                     /locus_tag="ACG8XG_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFQ0774580.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1772 a         1640 c         1159 g         2160 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac actctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt c
//
LOCUS       DAZKSR010000130         6642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000130 DAZKSR010000000
VERSION     DAZKSR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.133"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8XG_002701"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8XG_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8XG_002702"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8XG_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0774581.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8XG_002703"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8XG_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8XG_002704"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8XG_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0774582.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8XG_002705"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8XG_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0774583.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8XG_002706"
     CDS             3582..4271
                     /locus_tag="ACG8XG_002706"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0774584.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8XG_002707"
     CDS             4285..5787
                     /locus_tag="ACG8XG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0774585.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8XG_002708"
     CDS             5800..6276
                     /locus_tag="ACG8XG_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0774586.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8XG_002709"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8XG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag at
//
LOCUS       DAZKSR010000131         6569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000131 DAZKSR010000000
VERSION     DAZKSR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.134"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8XG_002710"
     CDS             127..579
                     /locus_tag="ACG8XG_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774587.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8XG_002711"
     CDS             593..1282
                     /locus_tag="ACG8XG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774588.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8XG_002712"
     CDS             1407..3758
                     /locus_tag="ACG8XG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0774589.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8XG_002713"
     CDS             3838..4233
                     /locus_tag="ACG8XG_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774590.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8XG_002714"
     CDS             4558..5175
                     /locus_tag="ACG8XG_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774591.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8XG_002715"
     CDS             5216..5515
                     /locus_tag="ACG8XG_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0774592.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8XG_002716"
     CDS             5549..5716
                     /locus_tag="ACG8XG_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0774593.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACG8XG_002717"
     CDS             complement(5761..6366)
                     /locus_tag="ACG8XG_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774594.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2109 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaatttt
//
LOCUS       DAZKSR010000132         6512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000132 DAZKSR010000000
VERSION     DAZKSR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.135"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8XG_002718"
     CDS             complement(99..1229)
                     /locus_tag="ACG8XG_002718"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0774595.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8XG_002719"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8XG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774596.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8XG_002720"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8XG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0774597.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG8XG_002721"
     CDS             complement(3485..4549)
                     /locus_tag="ACG8XG_002721"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0774598.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8XG_002722"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8XG_002722"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774599.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8XG_002723"
     CDS             4880..5692
                     /locus_tag="ACG8XG_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774600.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8XG_002724"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8XG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774601.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1968 a         1323 c         1017 g         2204 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tt
//
LOCUS       DAZKSR010000133         6511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000133 DAZKSR010000000
VERSION     DAZKSR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.136"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..826)
                     /locus_tag="ACG8XG_002725"
                     /pseudo
     CDS             complement(53..826)
                     /locus_tag="ACG8XG_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            862..2049
                     /locus_tag="ACG8XG_002726"
     CDS             862..2049
                     /locus_tag="ACG8XG_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFQ0774602.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            2782..3750
                     /locus_tag="ACG8XG_002727"
     CDS             2782..3750
                     /locus_tag="ACG8XG_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0774603.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3818..4576
                     /locus_tag="ACG8XG_002728"
     CDS             3818..4576
                     /locus_tag="ACG8XG_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0774604.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4644..5354
                     /locus_tag="ACG8XG_002729"
     CDS             4644..5354
                     /locus_tag="ACG8XG_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0774605.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5390..>6511
                     /locus_tag="ACG8XG_002730"
     CDS             5390..>6511
                     /locus_tag="ACG8XG_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0774606.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVI"
BASE COUNT         2191 a         1001 c         1329 g         1990 t
ORIGIN      
        1 gccaataaaa cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt
       61 caaagatcag accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac
      121 caaacgcaat tcgtttaatc agcttgatct tgttatacag cccctcagtg acgccgtttg
      181 agtgggacac cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt
      241 tctttttaaa cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagccta
      301 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
      361 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      421 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      481 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      541 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      601 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      661 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      721 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      781 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      841 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      901 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      961 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
     1021 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
     1081 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
     1141 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
     1201 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
     1261 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1321 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1381 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1441 agcgagatag agagagaacc tagagacgga aatgatagta tagcgtatga cattgattac
     1501 caattacctg aaggtagtcg tagtcgtatt aattatgagt atttaaacac aaacttcaaa
     1561 aaatacatga atataaaaga agaacgtgat tcgaatttaa ttcaatcatt gttaaacgac
     1621 ttacatcgca catttgcgca attaccagtg gagttacagc catatgctga gatgattcta
     1681 aatgatgtaa ataatgggga attagttatc aaagaaggat gggaatttac tgattacctc
     1741 aatgaatatc agcttagtga agaggacgat tttatccaag atttagtgga ttcaaccaat
     1801 attaatgagg cattattacg tgagatgttg gatttacgct taaccgaggt aaacattaat
     1861 gaatatagtc gatttgataa attaaaatca agtgttaacg taggtcattt cagtggatat
     1921 atcgagaaaa atttaggtaa aatggtaata ccaatcaagg taaatatgct aattgacaga
     1981 ttattgcgtg catttttatt agaagatgta ttcgatgtta cagagtacat aaagaattac
     2041 tttaactaga gtacacctac atcttatagt acataaaaaa attcatcttt tttagtttaa
     2101 taattcacca aaaaggtgag taatgccatt tttgaaaatt gagcttgttt tatgtctaca
     2161 tctgtatccg tatataatcc aaaggattat aaactcagaa gagtctttat tttttctgaa
     2221 tataactact gtatcaaaaa caatcgatta tgagacaaaa caaaatgacc tcttttattt
     2281 aagaaaggag gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa
     2341 tcaattttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
     2401 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
     2461 ccagtagttt cctaattcaa ctgtgtgcaa aacgatttct tctaaacaaa tcaagacaaa
     2521 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
     2581 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
     2641 attgacaaac acaaagaaag cggatacaat acaattatgt cacatgacgt tatatgacgt
     2701 tatgtaatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
     2761 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
     2821 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
     2881 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
     2941 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
     3001 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
     3061 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     3121 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     3181 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     3241 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     3301 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     3361 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     3421 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     3481 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     3541 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     3601 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     3661 agtattcttt cagaatatcg ttcagcttta gcagatgcac gcaatcatga tttagatgtt
     3721 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     3781 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     3841 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     3901 ctcagatgag gaggcagctc atgttttatc agcaataaaa aaaataggac tggacaacag
     3961 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     4021 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     4081 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     4141 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     4201 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     4261 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     4321 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     4381 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     4441 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     4501 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     4561 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     4621 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     4681 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     4741 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     4801 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     4861 tcaaccggca taaagttttt cgtaaaattg aatatgttat gggtttcagt gagagttgta
     4921 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     4981 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     5041 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     5101 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     5161 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     5221 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     5281 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     5341 gtttttactt atgatttcct gttttgattt caaggagtag attttattta tgaagcaagt
     5401 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggtt atattgtaat
     5461 caatgaaggg atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga
     5521 aaacacggaa ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca
     5581 tgtatttttc agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc
     5641 agctaacgag gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc
     5701 aatttatgct catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt
     5761 gaatgaaata aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg
     5821 gatgaatgct attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag
     5881 tcgagtatta ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc
     5941 tgaatttgaa gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga
     6001 tgacgcagaa tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca
     6061 ggcagtccaa gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc
     6121 tgaaaaggtc cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag
     6181 tggagatatt tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc
     6241 aattaaagca gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga
     6301 aggagataaa gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc
     6361 tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa
     6421 aatgaacgaa ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac
     6481 gttagaagag gcttacatgt caactgttat a
//
LOCUS       DAZKSR010000134         6468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000134 DAZKSR010000000
VERSION     DAZKSR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.137"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..789)
                     /locus_tag="ACG8XG_002731"
     CDS             complement(16..789)
                     /locus_tag="ACG8XG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0774607.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(786..1691)
                     /locus_tag="ACG8XG_002732"
     CDS             complement(786..1691)
                     /locus_tag="ACG8XG_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774608.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(1845..2165)
                     /locus_tag="ACG8XG_002733"
     CDS             complement(1845..2165)
                     /locus_tag="ACG8XG_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0774609.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            2639..3325
                     /locus_tag="ACG8XG_002734"
     CDS             2639..3325
                     /locus_tag="ACG8XG_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0774610.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            3327..4271
                     /locus_tag="ACG8XG_002735"
     CDS             3327..4271
                     /locus_tag="ACG8XG_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0774611.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            4470..5114
                     /gene="pcp"
                     /locus_tag="ACG8XG_002736"
     CDS             4470..5114
                     /gene="pcp"
                     /locus_tag="ACG8XG_002736"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0774612.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            5193..>6468
                     /locus_tag="ACG8XG_002737"
     CDS             5193..>6468
                     /locus_tag="ACG8XG_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774613.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         2086 a         1159 c         1305 g         1918 t
ORIGIN      
        1 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
       61 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
      121 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
      181 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
      241 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
      301 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
      361 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
      421 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
      481 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
      541 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
      601 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
      661 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
      721 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
      781 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
      841 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
      901 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
      961 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     1021 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     1081 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     1141 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     1201 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     1261 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     1321 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     1381 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     1441 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     1501 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     1561 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     1621 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     1681 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     1741 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     1801 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     1861 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     1921 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     1981 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     2041 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     2101 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     2161 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     2221 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     2281 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     2341 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     2401 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
     2461 tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat aatttttgtg
     2521 ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa acaactatat
     2581 tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag gaaaatatat
     2641 ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt
     2701 agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg
     2761 cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat
     2821 ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc
     2881 ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg
     2941 gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt
     3001 tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca
     3061 tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt
     3121 ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa
     3181 agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat
     3241 cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa
     3301 atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag
     3361 gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag
     3421 taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg
     3481 tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga
     3541 tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc
     3601 catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag
     3661 ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag
     3721 tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata
     3781 atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa
     3841 gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga
     3901 cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca
     3961 tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca
     4021 tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag
     4081 cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg
     4141 ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga
     4201 tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc
     4261 tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct cttctaacat
     4321 tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta tgttatttaa
     4381 actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag ataaccatat
     4441 gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt tttgatccat
     4501 ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca
     4561 gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag
     4621 tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg
     4681 ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca agaatccctg
     4741 ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca gccgcttact
     4801 ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt
     4861 acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt ttgcttcatt
     4921 tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca ttctcacccg
     4981 atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac attgcttctt
     5041 ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc ggaaatgccg
     5101 gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg ttagtagcca
     5161 gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc gtctttgaag
     5221 aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa caatacacaa
     5281 gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata
     5341 agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta gctgcaacag
     5401 attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga agccagcaaa
     5461 aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa tcgccaaaaa
     5521 ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat gtctctccga
     5581 cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta gacgaattat
     5641 tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa tactgttttg
     5701 tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa ttagctcagc
     5761 ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat caagcattgg
     5821 aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag cgacagataa
     5881 aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa gaattcttga
     5941 tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag
     6001 tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca aaacgtcaac
     6061 gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga ttcatccaac
     6121 caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca gaagggttct
     6181 tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt aaagaacgag
     6241 aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa tggcaactag
     6301 tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac
     6361 attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag ttagaagaat
     6421 cactgcgttt caatgatgag gcaagaaagt atctgaagga attactat
//
LOCUS       DAZKSR010000135         6347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000135 DAZKSR010000000
VERSION     DAZKSR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.138"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG8XG_002738"
     CDS             290..1135
                     /locus_tag="ACG8XG_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0774614.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACG8XG_002739"
     CDS             1416..2636
                     /locus_tag="ACG8XG_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0774615.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACG8XG_002740"
     CDS             2633..5353
                     /locus_tag="ACG8XG_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0774616.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACG8XG_002741"
     CDS             5337..6281
                     /locus_tag="ACG8XG_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0774617.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAZKSR010000136         6224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000136 DAZKSR010000000
VERSION     DAZKSR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.139"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG8XG_002742"
     CDS             complement(207..1169)
                     /locus_tag="ACG8XG_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0774618.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG8XG_002743"
     CDS             complement(1171..2610)
                     /locus_tag="ACG8XG_002743"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0774619.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG8XG_002744"
     CDS             2795..4741
                     /locus_tag="ACG8XG_002744"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0774620.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACG8XG_002745"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACG8XG_002745"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0774621.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 atgaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DAZKSR010000137         6145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000137 DAZKSR010000000
VERSION     DAZKSR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.140"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..548
                     /locus_tag="ACG8XG_002746"
     CDS             48..548
                     /locus_tag="ACG8XG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0774622.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            645..2492
                     /locus_tag="ACG8XG_002747"
     CDS             645..2492
                     /locus_tag="ACG8XG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0774623.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2495..3391
                     /locus_tag="ACG8XG_002748"
     CDS             2495..3391
                     /locus_tag="ACG8XG_002748"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0774624.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3441..3830
                     /locus_tag="ACG8XG_002749"
     CDS             3441..3830
                     /locus_tag="ACG8XG_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0774625.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3817..>6145
                     /locus_tag="ACG8XG_002750"
     CDS             3817..>6145
                     /locus_tag="ACG8XG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0774626.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2093 a          913 c         1311 g         1828 t
ORIGIN      
        1 tactcaattt gtttaaccga attgtcggtg cctgaggggg aatcacaatg gaacagatac
       61 gtgtttatat tgcgaacctt ggaaaataca atgaaggtga gttggtgggg gcatggttta
      121 ccccacccgt cgattttgat gaagtgaaag aacggattgg attaaatgac gaatatgaag
      181 agtacgcaat tcatgattat gaactgcctt ttgagattga tgagtataca ccaattgagg
      241 aaattaatcg gttgtgtggt ttagcggaag agcttaaagg aacgccaatt ggtgaggtcg
      301 cttcagagat tcaacatgcc ttcttcaatt ctttcgagga aatggtagag catgtggatg
      361 atatcatcta ttatcccgat tgtaatgaca tgagtgatgt agcgtattac ttgattgtag
      421 aagctggtga tttaggcgaa gtaccagaac acttgaagac gtatattgat tttgatgcgt
      481 gtggtcgaga cttggagatt ggcgggaatt atttggttac gagtcgaggg gtttttgaac
      541 taatgtaaat catttgttag aattgaccag aaatttctat cgtactttta cttttctatt
      601 tgatatactg aaatagaaaa tatttgttga taggggtgtt actaatggtt ggcaataagt
      661 ggtacaaatg tgatcttcat attcattcaa ctgcgagtca gtgctttcag gataaaaagg
      721 ttactgctaa ggaatgggta gaagaatgtt taaacaaagg actcgattgt gttgcattaa
      781 ctgatcacaa tacaggggaa aatattgatg aatataagaa agtggctatc gaaaatggct
      841 tagatttttt tcctggagtg gaattaacgt gtggagaaaa tggaactcat ttactagtcc
      901 tatttgataa aagtgcatgt acaaatgata tctctgattt cttaaccaag atgggtataa
      961 aaagagagga ttgggccagc gctgcagctt gctcaatgaa atcagtttta gaagttattg
     1021 attttgctga gcacgagggc ttgctcgtta ttcctgcaca tatagatgag tttaatggaa
     1081 tttcttcagc aaaatttgga aatctaggaa agatatttga atcagaaaat atatatgcag
     1141 tacagacggt tcaaaaagaa ttttttgaaa atcgctcaca aatgattccc tcatctaagc
     1201 gtgacactat ctatgatagt gtgaatgcca gatatgatgg tagagttgga aaagatactt
     1261 tggaatcttg gtataaatca gttgttgagg cagaaaaaaa taatatgaca tttcttagct
     1321 tttctgataa cccacactcg aaaggaaatt caaaacatgg actttgggga ataggcacaa
     1381 gatattccta tataaaaatg aaggacgaac ctgacttggg atcacttcgc gatgctttaa
     1441 tgctaggcga aactcgagtt cattctgatt tttctaattt ctccattaat gaaaatgaag
     1501 ttttattgga aaaattaagc ttttctggga ccactcttag cacaaaagaa gttgtagtcg
     1561 aattcagcga taatttaact tcaattattg gaggaagagg aacaggcaaa tcttgtatta
     1621 caagattctt ggtctatgtc cttggaaagg aagcagaact tgatcaattt tctgaaatac
     1681 aaagtgatta tcagaacttt gcacaaattg aacataatgg ctctggaata tttttgaagg
     1741 atacaattgt gaaactgaat attttctata aggggacaaa atatcaaata ataagaacac
     1801 aggatagaca ttctatttat gaatttacac aagagaatgg tctggttgaa aaagaaacag
     1861 aacggttgag aatgatttcc gataaggtga gtatatactc tcaaaaacaa atttatgaaa
     1921 tttcaaaaaa tcaaactagt atactagaat tactagatgg atataactct gatttgattt
     1981 cagagtataa aaatgagatt gaaacatgtg taaacgaaat aagaaaatta aattgggata
     2041 ttgtttctgt gaaaaaagaa atacaagata aggcgaaggt agagcttgaa attgaagatt
     2101 taaaaaaaca agtagaaaag ctatctcata agagttataa agatgtttat gatgagtatt
     2161 caaaagagac agatatttat agggaactga aacgtgatgt ggaggcactt aaggaaattc
     2221 ccaaaaatct aactgatagt gttgacaaaa ttgactttgg caatggtata aaagttacag
     2281 acgaaattga tgaacaccgc ggagaattga ttaagaattt agtgaccaat cgggctaaga
     2341 ttcaaacaat aattaatgaa atgtcagatg aaattgatag ttatagagca gagctgaata
     2401 aatcagattg gaaagttaac tataatgagg tttcaaagag gtatcaaaga ggtatcaaaa
     2461 acttagtgaa gagttatctc aagatgaatt aaaaatgtta caaaaccttg attctttata
     2521 tcaatcttta tcaaaaactg aagagcgatt ggaagaaatt attcaacaag aaataaagct
     2581 taaagattac caagttgagg ttgactcagc aatgtcaaga atgaataaat catatcaaaa
     2641 tctctattct gagagaagta gttcctcgcg cgaaattttt tctgatatta caggaataga
     2701 ggctactatt caaccaaata gggatttcaa taattatgtt aaagagctca gagcttgtat
     2761 taataaggag gacacttttg acagtgagtt tggatctctt attgataagc tgacaaaaaa
     2821 ttccattact aatcaagata tatataatga catacttgat ataagagtta gtggggaaag
     2881 tagtattttt tcagataaaa aattaatcaa ttctataaga aaattgaatg atgcacaagt
     2941 tcttgaatta caaaccctta agccaaatga tttagtagtt atccgacttg aactaaatgg
     3001 aaaaaaagtt agtttatcga atgctagtgc tggtcaaaag acatctgcaa ttctttctat
     3061 gattttggcg tatggaaatt caccactcat attggatcag cctgaagatg atttggatag
     3121 tcaattaatt aataacttaa tcgtaagaag tatcattaca aaaaaggaaa acagacaaat
     3181 tttaattgta actcataatg caaatatccc agttaatggt gattcagaat ggttgattag
     3241 catgggagat tcaaaagaaa tatcggctga tcagtgcggg tcggtagatg aacccaatat
     3301 caagccaaga atatgtactg tgatggaagg tggcgaggat gcttttacaa atagagcgaa
     3361 acgctatggt tttaaacaaa ttgtagaata acactagagg gtgagacata ttattatgtc
     3421 cgcccttttt aggaggaaaa atgaaaaaaa taaaaagcta taccagtatc tgggcagttg
     3481 aaaaagtcat ttacgcaatc aatgattttc agttgccttt tcctgtgact tttaaccaaa
     3541 tggcttggtt cgttttatct ttactgtttg tcatcgtatt tgcccatgtg cctccactat
     3601 ctatgataga ggaggtcttt ttaaaatact taggcatacc ggtggctgtc acttggttta
     3661 tgtcgcaaaa gacgtttgat ggcaagaaac cactgggatt tttacgatcc tttatcagct
     3721 atcacttgcg atttaaggtg acttttgcag gaaagaaagt caaagaacag aagaaacgat
     3781 gggatgaacc cagtactctt gtaaggagtg tgaactatgt atccgattaa gtatattgaa
     3841 aataatctcg tctttaacca agaaggcgag tgttttgcct actatgaatt agtgccttac
     3901 aattactcct ttttgtcacc cgaacagaaa tttcaagtgc atgataactt tcgccaacta
     3961 attgcgcaaa atcgtgaagg caagatccat gcgttacaaa ttgccacaga aagtagtatt
     4021 cgcgccacac aagaacgatc aaaaaaagaa attaccggta gactcaaaga agtagcgaaa
     4081 caacgaattg atatacagac agatgcatta gtatccatga ttggcgatag tcagattgat
     4141 tatcgctttt ttattggctt taaactaatt gcgacagatg aagaagtcaa tctaaagagt
     4201 ctcaagaaat ctttcttttc tgggcttcaa gaatttgtct atggagtgaa tcatcatttg
     4261 atgggcgatt ttgtttcctt atccaatgaa gaaattcgct gctattctaa gcttgaaaaa
     4321 ttaatggaaa gcaaattggc tcgaagattt aaagtcaggc gagtcacacc gagtgacctg
     4381 acgtatttga ttgaacatat ttatggcgaa aaagggatac cgtttgaaga atacgaattt
     4441 cagctaccaa agaaaaagct aaaatcagaa acactggtga aacgttatga cttattacgt
     4501 ccaaatcgtt gtttaattga agaaaaacca aggtgcttac ggatggaaca tgagaatcat
     4561 gagtcgtacg tagcgtattt aacaattaat acaattgtgg gggagatgga atttccttcc
     4621 tcagaacttt tctattacca gcaacaacaa tttacattcc caatcgacac aagtatgaat
     4681 gtagagattg tgacgaataa aaaggcttta gctaccgttc gtaataagaa aaaagaactg
     4741 aaagatttag acaatcatgc ttaccaatct gacaatgaaa cgaattccaa tgtcttagat
     4801 gctttggatt ccgtggatga attggaaacc accttggatc agtcaaaaga atctatgtac
     4861 aaactgagtt atgtggttcg agtgagtgcc gaatcagtgg atgaactaaa gcgacgctgt
     4921 gatgaagtgc tcgattttta tgatgatacc aatgtaaaac tagtccggcc ctttggcgat
     4981 atgatggggc tacatgaaga attcttaccc tcaagtaaac gatatatgaa cgactatatt
     5041 cagtatgtta cgtctgattt tttggcgggg cttggttttg gggctactca gatgctagga
     5101 gaattagaag ggatttactt tggctacaat gtggatacag gtcgtaatgt ttacctgaaa
     5161 ccggcattgg cttcacaagg agtcaagggt tcagtaacca atgcgctggc cgccgctttc
     5221 ctgggttctc ttggtggagg taaatccttt agtaacaatc tcttagtcta ctatgctgtt
     5281 ttgttcggtg gtcaagctgt gattgtcgat ccaaaagggg agcgaggagg gtggaaagaa
     5341 accttgccag aaattgcgga tgaaatcagt atcctcaact tgacgagtaa gtcagaaaat
     5401 caaggtctgc ttgatcctta tgtgattatg aagaaaaaga aagactcgga aagtttagca
     5461 attgatattc tgactttttt aacaggaatt tcaagtcgag acggggagaa gtttcctgtg
     5521 ttacgtaaag ccattcgaac ggtgactcaa agtgatcaac gggggttatt acaagttatt
     5581 aacgaattgc gaaaagacgg gtcaccaatc gcggaaaata tcgccgatca tatcgagtcc
     5641 tttaccgatt atgattttgc tcatttgctt ttctcagacg gctcgattac ccaatcgatt
     5701 agtttagata aacagctaaa tatcattcaa gtggcggatt tggttttacc ggatgcagaa
     5761 acgacttttg aagaatatac caccatggaa ttgcttagtg tttcgatgct aatcgtcatt
     5821 tccacttttg ccttggactt tatccatagt aaccgagaaa tcttcaaaat tgtcgatttg
     5881 gatgaggctt ggagttttct tcaagtggct caaggaaaaa cgttatcgaa taagcttgtt
     5941 cgtgctggtc gggccatgaa tgctggggtc tatttcgtca ctcaaaatag cgacgattta
     6001 ttagatgaac gtttaaaaaa taatatcggg ttgaagtttg cttttcgcag taccgatatt
     6061 catgaaatca agaaaacctt ggaattcttt ggtttggatc aggaggatca aagcaatcaa
     6121 aaacgcctgc gagatttgga aaacg
//
LOCUS       DAZKSR010000138         6058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000138 DAZKSR010000000
VERSION     DAZKSR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.141"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..123
                     /locus_tag="ACG8XG_002751"
     CDS             <1..123
                     /locus_tag="ACG8XG_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0774627.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(209..1303)
                     /locus_tag="ACG8XG_002752"
     CDS             complement(209..1303)
                     /locus_tag="ACG8XG_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0774628.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1492..2253
                     /locus_tag="ACG8XG_002753"
     CDS             1492..2253
                     /locus_tag="ACG8XG_002753"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0774629.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2368..2808)
                     /gene="fabZ"
                     /locus_tag="ACG8XG_002754"
     CDS             complement(2368..2808)
                     /gene="fabZ"
                     /locus_tag="ACG8XG_002754"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0774630.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3028..3783
                     /gene="fabI"
                     /locus_tag="ACG8XG_002755"
     CDS             3028..3783
                     /gene="fabI"
                     /locus_tag="ACG8XG_002755"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0774631.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3893..4945)
                     /locus_tag="ACG8XG_002756"
     CDS             complement(3893..4945)
                     /locus_tag="ACG8XG_002756"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0774632.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            5071..5991
                     /locus_tag="ACG8XG_002757"
     CDS             5071..5991
                     /locus_tag="ACG8XG_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0774633.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1858 a         1204 c         1078 g         1918 t
ORIGIN      
        1 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
       61 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      121 tgacaaatca taacctataa aaaaacaaaa taaaaaagca gaaataactg gctatttcta
      181 gcggattatt tctgcaataa ctaattatct attttttatt ttcaattaat ttaaagcttg
      241 accatggttt caaaaagtca aggaggaata attcgtcatc ggagattctt cctacaacat
      301 tgacacgtcc gtcgtttttc atatccttca aggcgatttg tgtttctcct ttgtattgac
      361 cgtacccttc attgtcaatc agtacatccc catgatgcat atctcgcgta ttatgaggcg
      421 ggaaatcttt atctttgtag tatacacgag tcatcgttga acgaaggata tattctgagc
      481 gatcccctcg ataactatga aggttatcaa acaagcagat tcgttcgttt tctgtaatac
      541 cttcttccgt atctactttc aatgttggat aatccgcgtt aaatgcttct gccatttctt
      601 tcaattcttc ttcacttgcg tacgcatttc caatagaaat atcatcaatc aatcctgtaa
      661 ggattaaatg cttcacttgt gtcgcaattt caagatctcg gtggtcttcc agcgtgcaca
      721 agccatcttg cgtaggccaa gggccaaact cagcagattg cgaattgacg aatgccatcg
      781 tattcaaatt atattttctg aatttttctg aacaaaaaac aaagtgttcg tatcctaaac
      841 cagagtatcg atgaggataa aaattatgag aacctaataa attatccgtg tttggcgaat
      901 aactcatgat gttgtcaacg taattcgtgc ccgaactcat attgatctcg attttgatac
      961 caaaaggatt gcgcgtcatt tttgcttctt ctgctcctgt gaatccgata tctaagcgga
     1021 ccccgtaggc acccatctta tggaaaaaag ataagtcatc gtaagaaatc tccaactgtt
     1081 caaataacgc tggattgata tcaaccatta cttccatgcc taggctattc gcgtaatcaa
     1141 ctacttcttt aaattctgaa agaacttttt ctttatcatc attgatctgc agcaaactgg
     1201 taaaaacccg tttataccca tacttatgag ctaaatccaa gtacgctttg tccttttcaa
     1261 atgttgaccg ttctggataa atcgagattc ctaattttcc catattacca tttcctcctg
     1321 catcttttcg tttttgtatc cacttacatt atagcaaaca caaagtaaat ggtctacacc
     1381 ttttcttttt cttttaaaat aaatgataaa ttccaataat ttaagtaggt attttccctc
     1441 tattttgtta tcattaaaga taacaaaata gtaaattgga aaggaacagt tatgaagaaa
     1501 aataaagtta ttttaagtat tagtttagca gcagcattgc tgtttgctgc tggttgttct
     1561 gcaaacaaca cgacttcttc tagttcgtct acagaaagtt caaaaagtac atctactgcg
     1621 gaaagcacga ctgccagtag cgtcgatttg aacgcattgg aattaccaca attgacgaaa
     1681 gatgtcgccg aaaatgaaga tctggtcgaa atggtgacga ataaaggcac aatcaaaatc
     1741 aaattatttc ccgaacacgc accaaaagcg gttgaaaact ttatgacaca tgcaaaagac
     1801 ggttactaca acggactgac tttccatcgc gtcatcaaag atttcatgat ccaaggtggc
     1861 gatcctaaag gcgatggcac tggaggggaa agcatttggg gagaaggatt tgaaacagag
     1921 atcaacaacc acctttataa tataagaggt gcgttatcta tggctcgctc acaagatcct
     1981 aacagtaacg gcagccagtt ctttatcgtt caaaatacag atgatatgca tgatggtctt
     2041 ttaaaagacg actatcctca agcaatcatc gacgcttata aaaacggcgg ttatccttca
     2101 ctagatggcc attatactgt cttcggccaa gtgattgaag gaatggatgt cgtggatgct
     2161 attgcaaata tcgaaacaga tagttcagac aaaccaaaag aagaagtaaa aatcgataaa
     2221 atcaatatac tgcaagaagc aaaacaaaaa taaaaaaata acgtctgaat cctgcataga
     2281 tgacaacgta tgctaggatt caggcgtctt ctttttacat aaaaagcaag taagcttcct
     2341 tttgttttgc tatatcaggt tttatagtca agcattttgg ttgacgatga acgtgaactc
     2401 gcactcacag acttttttat catctacata tgctgcagat aacgctgtac cgactgatcc
     2461 cttcattttg gtgatttcaa aatgacattt cagcacatct cctggaacaa ctttttgacg
     2521 gaatttggcc ttattgatcc caccgatata agctcttttt cctagaaact cttctgattt
     2581 taggataagt atcgaacctg cttgtgccag tgtttctaga atcaacgcac ccggcatcac
     2641 tggattgtca ggaaagtgac cttggaaaaa atcttcattg attgttacat tttttgtcgc
     2701 aacgatcttt tttcctggtt ctaaagtatc tacataatca atataacaaa taggataacg
     2761 attaggaatc aattccatta cttcagtagc agataatacg taagacataa ttaaactcct
     2821 tctttcaggc aattgccatt ttcattactt tgactatcaa agtatcatat tcctaaatat
     2881 ttagcaatct ttttttaatt taagaaaaaa tagtttgaca atcaaaacat tttgactaat
     2941 aattaagaaa tcaagaagtt attttagcta ttattctcta tttttttacg actatcaaat
     3001 ttttatcaga aacaaaggag agtcattatg tcatttctag aaggaaaaaa aatcgtcatt
     3061 atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg ctgaagcaat gttgaaacaa
     3121 ggggcagaaa ttatctatac ttaccaaaat gaacgaatga aaaaatcatt agtcaaattg
     3181 gtcggcgaag aagcattcac tgtagaatgt gatgtttcta gcgacgaaag catcgaaaaa
     3241 gcaatgaatg ccatcggtga atatgctggg gagatccacg gccttgtcca tgcagttgct
     3301 tacgccaaca aagaagaatt gtccggaaat gtgtctgata tttccagaga cggctatcaa
     3361 ttagctcaag atatcagcag ctactcttta attgctgtat caaaatacgc acaaccgttt
     3421 cttgcaccaa acagcgggat cgttagcatg acttatctag gttcagaacg cgcaatcccg
     3481 aattataaca tgatgggtat tgcgaaagct tctcttgaag ctgctatccg ttatttggca
     3541 gctgagttct cgcctaaagg aattcgggtc aatggaatct cagctggggc aatcaaaaca
     3601 ttagctgtca caggtgttaa agactatcaa aaactgatcc agttatctga atctcgtacg
     3661 cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg cttgtgcttt cttaatcagc
     3721 ccactttcca gcggaatcat cggtgatatt atctacgttg acaaaggtgt ccatctttca
     3781 taaacttttg ttgcattctt taaagtaaaa catataaaaa tcaggaaaaa actttttaaa
     3841 gtctcttcct gattttttct ttttacgttt ttttcaactc attactttga aattattcca
     3901 cgacttcaat ccagccttct ggtgcttcta tgtctccaaa ttgaatacct gtcaattcat
     3961 catacaaccg ttttgttact ggtcctactt ccgtttcact ataaaataca tgaagattgt
     4021 tattgtgata aatcccaccg attggagaaa taacagccgc tgtaccacat gcaccagctt
     4081 ctgcaaattg atctagatca tcaatgaaaa tatctccttc aattgcttgc agtcctagtc
     4141 gttcttcagc taactgcaac aaggaatatt tcgtgatact tggtaagata gagggcgatt
     4201 gcggagtgat gaattttccg tcttttgtaa ttccaaagaa atttgctgca cctacttctt
     4261 cgattttcgt atgtgtggct ggatcaagat aaacacaatc tgagtaatcc agttcttttg
     4321 cttctgttcc tggaagtaaa ctcgctgcgt aatttccgcc gacttttgct gcaccagtcc
     4381 ccttaggagc agcacgatcg taatcagata caataaaatt cgttggtttt agtccacctt
     4441 tgaaatacgc tcctactggc gtacagaaga cgacaaataa atattctgga gctggatgga
     4501 caccaatgtt ttcaccgacc ccaatcagta aagggcgcaa gtataatgta gcgcctgttc
     4561 catagggcgg cacaaaatct tcatttgctt ttaccacttg cttgacagca tcaacgaaca
     4621 tcttttcagg gaccattggc atcagcaatc gtctagcact ttgattcatc cgtcgactat
     4681 tttgttcagg tcgaaagaga ttgaccttgc cgtcttttcg acgataagct tttagacctt
     4741 caaaacattg ctgaccataa tgaagcgctg gagagccttc atgaatcgtc aggtaattat
     4801 cttctgtcaa ttctccttct tcccattgtc cgtctttcca tttggcaata aaacgccaag
     4861 gagttttgat ataagaaaaa cctagattgt tccaatcgat ttgcatatac ttaccacctt
     4921 cgttattttc tccacatttt atcattatcc atattatttg tcattttatt ttcagctttt
     4981 ttccttcatt atagttattt ttgctatttc cttttttgaa aacttcttgg tacaatacag
     5041 aaagaatcaa attttagaaa agaggatttt atgttttcat tattagcaac tgccggtacg
     5101 acagactctt ctggcgagac attgacagat caagctgtca gcaaagtaaa tgcgtttcag
     5161 cggttttggg acgggattga ttgggacgcc attttggctg cctttatcca aaaaagttta
     5221 tatctgattt ttttgatcgt ccttttttgg attcttcacc gtgtgggaaa atatctgatt
     5281 gataagtctt ttaatcagta ttctaaaaaa gccattctaa acgagagccg cttaaagact
     5341 ttacatagtc ttttaaatac tatttttcat tatacccttg gtttcttttt tatctatgca
     5401 attttatctg ctatcggtgt gccgatcgga tcacttttgg ctggtgccgg aattgcaggt
     5461 gtggcaatcg gtcttggtgc ccaagggttc atgagtgatg ttattaccgg attttttatt
     5521 atcatggaac aacagatgga tgttggggac tacatcaagc tagcaaactt aacgattgaa
     5581 ggaaccgtcg tatctgttgg tatccgtacc cttcagctga aagcaacgga tggtactgtc
     5641 catttcatcc cgaatcgaaa catcaccacg atcagcaatc tctctcgtgc aaatatgcaa
     5701 gtacttttag atattcggat tgttcctgaa gaaggatatg acaaaatcta tgagatcatt
     5761 gatcgagtaa atcgtacatt agctgaaaaa taccaagaag agctccaaac tgaaccttct
     5821 atctttggat tagtggatct tggaaacagc aattttgcga ttcgtacggt ctgctacgca
     5881 ttgaacggaa aacaatatgc cttaaaagaa gagttcttga gctcctatgt caaagaattg
     5941 acagcttctg gtttcacgat tccgaacagt ccaattgctg tcggaaaata atttggacaa
     6001 acgatgcaac gaatctcact aaaaagagcg tgggacaaaa gtaaagaaca cttttgtc
//
LOCUS       DAZKSR010000139         6052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000139 DAZKSR010000000
VERSION     DAZKSR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.142"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..481)
                     /locus_tag="ACG8XG_002758"
     CDS             complement(194..481)
                     /locus_tag="ACG8XG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0774634.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(501..725)
                     /locus_tag="ACG8XG_002759"
     CDS             complement(501..725)
                     /locus_tag="ACG8XG_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0774635.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1174..1344)
                     /locus_tag="ACG8XG_002760"
     CDS             complement(1174..1344)
                     /locus_tag="ACG8XG_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774636.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(1360..1899)
                     /locus_tag="ACG8XG_002761"
     CDS             complement(1360..1899)
                     /locus_tag="ACG8XG_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0774637.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(1920..2864)
                     /locus_tag="ACG8XG_002762"
     CDS             complement(1920..2864)
                     /locus_tag="ACG8XG_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0774638.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3501..3587
                     /locus_tag="ACG8XG_002763"
     CDS             3501..3587
                     /locus_tag="ACG8XG_002763"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0774639.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3708..4397)
                     /locus_tag="ACG8XG_002764"
     CDS             complement(3708..4397)
                     /locus_tag="ACG8XG_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774640.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4404..4682)
                     /locus_tag="ACG8XG_002765"
     CDS             complement(4404..4682)
                     /locus_tag="ACG8XG_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774641.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4642..5556)
                     /locus_tag="ACG8XG_002766"
     CDS             complement(4642..5556)
                     /locus_tag="ACG8XG_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0774642.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5538..5918)
                     /locus_tag="ACG8XG_002767"
     CDS             complement(5538..5918)
                     /locus_tag="ACG8XG_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0774643.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1613 a         1119 c         1012 g         2308 t
ORIGIN      
        1 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
       61 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      121 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      181 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      241 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      301 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      361 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      421 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      481 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      541 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      601 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      661 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      721 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      781 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      841 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      901 atgtcgacta tgccaagtag cccctctatc aagggctata gctgctcact ttgtgatttt
      961 taattggtct agctaaaagc aactttctaa agcttgattt ttcgcggtta tctatatcta
     1021 attctttgtg catttttatc tgtcattttt ttaagacttg ttattttttg taaaatatgg
     1081 taaatttttg gcagcgcttc ataagcgcgt gctttttcaa gaataaacac tcattatttt
     1141 ttaactggct gctcggaaga gcagtctttt ttattattta tccgtctctt ttacaaacaa
     1201 ttcttcgtta ggactgttgt aataatttgg attcaatttc aattcaccat gtttattccc
     1261 tttattgatt actgacaata acatcggact gaagcctaga attcctagta aaatagaacc
     1321 gaatagaaga ttttttcttg tcatgaatgt tccctccagt tatttccaaa gttgccacca
     1381 tttttgcttc ttcttttttg tgtgtttttc tatcgattct gtttcttcag aaagaatagt
     1441 agttgattca tcttctggag tttcttccgt ataagctaat tgtaactgtt cttgcagttt
     1501 ttcgatctgt tgatttgcct gtagggtgag ctgttgttgc tggtcgagta atttttgcat
     1561 attagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1621 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1681 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1741 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1801 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1861 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     1921 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     1981 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2041 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2101 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2161 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2221 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2281 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2341 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2401 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2461 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2521 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2581 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2641 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2701 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2761 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2821 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     2881 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     2941 atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataaaca
     3001 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3061 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3121 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3181 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3241 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3301 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3361 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3421 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3481 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3541 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3601 gacatcaacc tgaaaaaaag ccccagagaa tccaacctct ctggggcttt cgtgtatcta
     3661 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3721 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3781 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3841 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     3901 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     3961 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4021 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4081 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4141 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4201 cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata
     4261 acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt
     4321 tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag
     4381 ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc
     4441 atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc
     4501 tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt
     4561 gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca
     4621 ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc
     4681 aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt
     4741 tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     4801 ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt
     4861 ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa
     4921 ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc
     4981 acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg
     5041 aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg
     5101 tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc
     5161 atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt
     5221 ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt
     5281 gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt
     5341 ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac
     5401 acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat
     5461 gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct
     5521 acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg
     5581 atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg
     5641 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
     5701 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     5761 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
     5821 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     5881 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     5941 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
     6001 ctaaagctta ctaaagggct tggcaagctc tatatttgct agccaaatac ct
//
LOCUS       DAZKSR010000140         6019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000140 DAZKSR010000000
VERSION     DAZKSR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.143"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..362)
                     /locus_tag="ACG8XG_002768"
     CDS             complement(60..362)
                     /locus_tag="ACG8XG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0774644.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACG8XG_002769"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACG8XG_002769"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="ACG8XG_002770"
     CDS             complement(1119..1892)
                     /locus_tag="ACG8XG_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0774645.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="ACG8XG_002771"
     CDS             2036..2386
                     /locus_tag="ACG8XG_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0774646.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="ACG8XG_002772"
     CDS             2367..3083
                     /locus_tag="ACG8XG_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0774647.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="ACG8XG_002773"
     CDS             3083..4531
                     /locus_tag="ACG8XG_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0774648.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="ACG8XG_002774"
     CDS             complement(4602..5111)
                     /locus_tag="ACG8XG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774649.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5101..5825)
                     /locus_tag="ACG8XG_002775"
                     /pseudo
     CDS             complement(5101..5825)
                     /locus_tag="ACG8XG_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2045 a         1050 c         1170 g         1754 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataattt
//
LOCUS       DAZKSR010000141         5954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000141 DAZKSR010000000
VERSION     DAZKSR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.144"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACG8XG_002776"
     CDS             <1..813
                     /locus_tag="ACG8XG_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0774650.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACG8XG_002777"
     CDS             786..1229
                     /locus_tag="ACG8XG_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774651.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACG8XG_002778"
     CDS             2399..3367
                     /locus_tag="ACG8XG_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0774652.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACG8XG_002779"
     CDS             3384..3908
                     /locus_tag="ACG8XG_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0774653.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACG8XG_002780"
     CDS             4284..4565
                     /locus_tag="ACG8XG_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774654.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACG8XG_002781"
     CDS             4567..4983
                     /locus_tag="ACG8XG_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774655.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACG8XG_002782"
     CDS             5613..>5954
                     /locus_tag="ACG8XG_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFQ0774656.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAZKSR010000142         5868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000142 DAZKSR010000000
VERSION     DAZKSR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.145"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            641..1375
                     /locus_tag="ACG8XG_002783"
     CDS             641..1375
                     /locus_tag="ACG8XG_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0774657.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="ACG8XG_002784"
     CDS             1391..2560
                     /locus_tag="ACG8XG_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0774658.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8XG_002785"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="ACG8XG_002785"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0774659.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="ACG8XG_002786"
     CDS             3650..4576
                     /locus_tag="ACG8XG_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0774660.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5839
                     /locus_tag="ACG8XG_002787"
     CDS             4595..5839
                     /locus_tag="ACG8XG_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0774661.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYVFS
                     GGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIPFV
                     TAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIAIN
                     VLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1964 a          954 c         1203 g         1747 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tatgtttttt ctggtggtgc aggttgttta tttgcacttg ccattttaac
     5461 atttttcttt tcaaagagtc aggaaaataa aactttaggt aagttatcaa tcgctccagt
     5521 aagttttcaa gtagcagagc cccttctatt tgggtttcca actatattaa atataaaaat
     5581 gttaattcca tttgtaactg caccagtagt aacaacatta attacttatt tttcaatgag
     5641 tatgggatta gttgcaaaac ctgtaggagc aacaatccca tggacaactc caccgataat
     5701 tgccggattt ttagcttctg gtggacgaat aagtggtgca gtgattcaag taataacaat
     5761 tgcaataaat gttttgatat actatccatt ctttaaatta gatgataatg ccaagctaaa
     5821 aagcgaaaaa aatgattaga actacctata tgtttctaac tgtagtta
//
LOCUS       DAZKSR010000143         5769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000143 DAZKSR010000000
VERSION     DAZKSR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.147"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8XG_002788"
     CDS             complement(<1..300)
                     /locus_tag="ACG8XG_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0774662.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8XG_002789"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8XG_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8XG_002790"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8XG_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774663.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8XG_002791"
     CDS             1706..1942
                     /locus_tag="ACG8XG_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774664.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8XG_002792"
     CDS             1949..3901
                     /locus_tag="ACG8XG_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0774665.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8XG_002793"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8XG_002793"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0774666.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG8XG_002794"
     CDS             complement(4797..5489)
                     /locus_tag="ACG8XG_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0774667.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAZKSR010000144         5515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000144 DAZKSR010000000
VERSION     DAZKSR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.148"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..578)
                     /locus_tag="ACG8XG_002795"
     CDS             complement(120..578)
                     /locus_tag="ACG8XG_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0774668.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(591..1922)
                     /locus_tag="ACG8XG_002796"
     CDS             complement(591..1922)
                     /locus_tag="ACG8XG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0774669.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1933..3375)
                     /locus_tag="ACG8XG_002797"
     CDS             complement(1933..3375)
                     /locus_tag="ACG8XG_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0774670.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3362..4126)
                     /locus_tag="ACG8XG_002798"
     CDS             complement(3362..4126)
                     /locus_tag="ACG8XG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0774671.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4230..5105)
                     /locus_tag="ACG8XG_002799"
     CDS             complement(4230..5105)
                     /locus_tag="ACG8XG_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0774672.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5360..5444)
                     /locus_tag="ACG8XG_002800"
     tRNA            complement(5360..5444)
                     /locus_tag="ACG8XG_002800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5408..5410),aa:Leu,seq:caa)
BASE COUNT         1689 a         1160 c          816 g         1850 t
ORIGIN      
        1 aaattcgata attaacaaaa atcaaaatga ttaaatctag tgattgaaac atatcatttc
       61 aaaaaaattt tgtaaattca cccataaata tcaccctatt taactttttt catcgtacac
      121 taaaattcaa aacaactatc tttaatcttt acttcttggt tttgttttaa taaatttcct
      181 tcaaaaatgc ctggagaaac tacaatgatc accgtatctt tttgtgcttg ctccaacttc
      241 ttcaaatcta taatagctaa cagtgtccct tcttttacct cctggttact ttcaacccgt
      301 atctgaaacg gacttccttc gagttcaact gtatctaaac ccatatggat cagtattttt
      361 ctgccagatt cactttcgat cgttatggca tgtcgggtag gaaatatatt atcgattatt
      421 cctttaactg gcgcatggat atatccatca tgattcgtta tagcgtatcc aactcctatc
      481 atttttgaag aaaagacctc gtcttttact gctgttaatg gaataatttg accactagct
      541 acagctttta gaatgtttct tgttttaaag aaatgcactt tttatcacct ctactttaat
      601 gctggccaat attttttgtt tactttgatc atttcatcta gtattttttt tgcgactctg
      661 gcatttggta cggtttgatt caatgtaaat gcttgtaaag ctttttgata agaaccttca
      721 aagtaagcat ctactagtaa cttctctact gcaacttgtg cctccatcaa tcctttatga
      781 aaatcactga ttggttttct tagtgtaatt ggctccacac cagttgcacc aacatatgcc
      841 ggtatttcaa caactgcatc tgaccgtaga ttcggaatcg ccccttgatt aggaacgatt
      901 agcataaagc gacgacgatc atcattcaac aaggaaatag ctatatcgac gatatattgt
      961 ccatgctccc caaaattaaa gttcaatact tcttccacaa taccgcggcg tattttatca
     1021 gccatttctt tggtattttt ctctcgtcca tccatgacca tattagcacg tgtatactct
     1081 ttgtcagtat gctcaacgac catatcagga tataagtaat actctaagta attattcggg
     1141 atattacttg gaaaattttt cgtgatgact gacatcattt gaaaggtttt ttgccaagtt
     1201 ttatctccta tattaaaatc ggcgacttgc atttcttttg ttattagttt ttcaataatc
     1261 tctggcataa tttctcgttg caattcttta tcataaatcg atgtatacca gccaaagtgg
     1321 tttaaaccat aatacgtaac gatccaattc ttacgatcat aaccgtaatt cactgcaatc
     1381 gtttcttcaa ttgaaatagt catatcacag gcattaatca tttttacatt cggatattta
     1441 cgacgaacag cttcagaaac gatcgactct ggatttgtat aattcaaaat ccatgctttg
     1501 ggggcatagt cttgtatatg atcgacaatt tccaataaac ctttgattga gcgcatccca
     1561 taagcaaatc ctccgagtcc acacgtttct tgtcctacta atccatgttt aagtggaatc
     1621 ttttcgtcct tttctcgcat ctccaatcct ccaacacgaa tttgtgagaa aacaaaatcg
     1681 catcctgtaa aagcaagctt tggatcatcc gttgcacgaa tgcgaatatt tgaaaaacct
     1741 tttcttttca gcataaattt aacaatcaag tacatatcca tatttctttc ttcatcaata
     1801 tcatataaac gtatttcaga taatggcaac cgtttttcat tgttcaaaat ggcttgaacg
     1861 attcctggtg tatacgtact cccgcctcca gctatagtga taatctgttt tttcatcatc
     1921 atcttttcct ttctattctt ctaataattt attgaattct gccataacat aagtcacttc
     1981 taagccgatg ataatttgat aatttttttc agttggttta atgatcccgc ttatccctaa
     2041 ttgcttgatt cgctgttcct cgaccatggt gggatccact acctctaccc gtaggcgtgt
     2101 attacaacta tagacatcca ggatattggc ttttcctcca aacgcttgta gcatttttgt
     2161 tgccatatat tcataatttt tacttgctaa ccctaactct gtttcttcta aggttacttc
     2221 attgccaaac tctgccttag cttcacgtcc taatgtctga atgtttctct tattgatcaa
     2281 ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt ccgattggaa gaatcaaaat
     2341 agcattggta gcgattcgaa aattgatgag ataatcaaca atacatgctc cgaatgtaaa
     2401 accaattcgt atattgaata agtaacagac aatccctgct aaaccggcaa aaagtgcatg
     2461 gatcacgtac aacataggag aaagaaacat gaaactaaat tcaacaggct cagtgatatt
     2521 tgcaataaat gaagttcctg caccgcttcc catgattcct ttgaccatct ctttattttc
     2581 tttataagct gctttataga tagctagaca tatagctggt ataccaaaca tcattactac
     2641 aaaaaaaact gataaaaaca gtccagcctg aggatctccg ttgataaagc gtgtcatctc
     2701 tcctctgaaa acttccttgc taggagatgt atagcttcct aaatcgtaat aaatatacgt
     2761 attcaacaca tggtgcagtc caaaggggat cagcaaacga ttcaaaaaca taaatatccc
     2821 cacacctaat gctcccatat caacgagcag ttttgcaaaa ttatcaatcc caatttggat
     2881 gagcggccag ataaagccca aaaaaacagc tgcgattgtt tgtagaatca tcaccatagt
     2941 caatgggaat ttttcgcccg caaaataaga aaaaactact ggtaattttt gttctttaaa
     3001 gcgattatac gtccatgccg ctactaaacc ggaaataatt ccaccaaaaa tccccatatt
     3061 cacttctggg tccataattt ctaagccttt tttcaaggta gcaatcgcta aaaaaccagt
     3121 aagtgcaggt attcctttgt ccttattttt gacaaaaccg attgcaactc ctatagcaaa
     3181 taagatatcc aagtttccaa aaacggcatt tccagctgca ccaattactg ggatgttcag
     3241 catatcgtca gctgataaac gcatgatcaa tcccgctaat ggcatcgctg caactggaag
     3301 tagcatcgct cttccaagct tctgcacatt tgatttaaca ttcattttta ttcctcctct
     3361 attaaatatc ctcatatgtt actaactcaa tcaaaggatc atttttcact ttttgagcaa
     3421 atgctgaatg aattaaaaat tcgacttggt gggtacgagg atacacaaaa cttgaaccat
     3481 ttaacaattc ctgatccaaa tacgctgcgt gacacatgac ttcgatcgtt ttatcttctc
     3541 tcaatatcat gagtaaactt gccaaatatt cttccacact cgtattgatt tgtcgttttt
     3601 tttcatcagt ttctcctacg acatcgaaac aaggctcaaa ataatcgaca tgacgaactt
     3661 catcttttcg ttcaaagttg ctccgaaccg gcaaatcata tttttttgct aatgtaacaa
     3721 ctacctcctg attttcatcc aaagtatgag tatgatgatg actgtctaaa tgagttggta
     3781 tgatccccgc atgataaatt ttttgaatct gagcattcca ttcctgatac agttgatctt
     3841 tgtcaatgtt aaatggatgc tgataataag ataaggatct gaactctccg ctttcgataa
     3901 gagtatcgac agttttgagg agtggtttgc cacacgtcaa tgttaaatgg actcctacac
     3961 ctagcctagg catttccttt cttagtttca cagcatgctc gaaacccggc atatttgcca
     4021 tcaatgtagt tgatgtcaaa attcctcgct gataagcatc cataatccca taattcacac
     4081 catggctata gccaaaatca tccgaattaa aaatcacttt tcccatttca ttctccttct
     4141 tttttctact aaaataggcg caaccaaata atcatagtcc atttactatg tgtcatttgg
     4201 ttgcgcctat agatagttac gcccttattt taatagattc ttgctagatg aattgccaaa
     4261 tagatgattt cattttcttg aatggcaagt tgaaattgct gttcgatgat atctttaatc
     4321 tttagtgctg tacaatacgc tttgctatgt ttaaatgttt cagtaaaaaa accaatattt
     4381 tctactggcg tttcttcctg ataaatcaag cgtttaacca ggaatttgat atgaaccatt
     4441 aagcgatgat aagtcagtga ttctttatca taaacgtttg aataggattt ttctagatgg
     4501 gacaaaatcg tggtcgtcat ttctactatt tttaagctct ttaaactatt aggctctcct
     4561 agctcagcat taatgatatg catagtgatg aatcctactt catttgaccc taactcggtt
     4621 tgatatcgat gattgattcg ctcgaccact tctactgcta tatcatattc atttggataa
     4681 aggaatctca tttctggcaa cataattgct gtgacatctc tatcttctct ttttcgttga
     4741 atagcaaaat aaatatgatc tgtcaatgta atatagatat ttgaatctaa tggagaggag
     4801 atcctagctg ttatcagttc aatcacttct tccgcaaaat ccataaattc gattggaatc
     4861 tgcgagacta tctcactgaa tcgtttgatc ttttctttag aatctaatac aaataatttg
     4921 tcagcatcct caacatcaaa tggcttactc actttagcag aaaagccaat ccctttcccc
     4981 atcgcaattt tttctattcc atcgtcatcg atcaatacag cattatgatt cagctttttc
     5041 atcacataca ccgttaattc acctcctcta tatgataaaa caaaacaact tgcagactcc
     5101 ttcatacata tttttgtctc caaacaattt ttaaagacaa aaaacgacct aagaaagaac
     5161 cctttcttag gtcgtgccta attgaatagt agcaccctac ataatttaca aaaatagttt
     5221 aacacataat aaaagcgtta tcaatatctt ttttactcgt tttatgataa tacttgtatc
     5281 gaattgattt ggaggtaatt acataaaaac aaaaaaaata tagcaactct ctataagaaa
     5341 gttgctatat tgataattat accggcggcc ggggtcgaac cggcacgtcc tcaacggaca
     5401 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaaattt aaaaataata
     5461 actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg cctta
//
LOCUS       DAZKSR010000145         5420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000145 DAZKSR010000000
VERSION     DAZKSR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.150"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG8XG_002801"
     CDS             279..1127
                     /locus_tag="ACG8XG_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0774673.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG8XG_002802"
     CDS             complement(1298..1639)
                     /locus_tag="ACG8XG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFQ0774674.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG8XG_002803"
     CDS             1725..2432
                     /locus_tag="ACG8XG_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0774675.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG8XG_002804"
     CDS             2419..2742
                     /locus_tag="ACG8XG_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0774676.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG8XG_002805"
     CDS             complement(2831..3868)
                     /locus_tag="ACG8XG_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0774677.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG8XG_002806"
     CDS             complement(4315..5298)
                     /locus_tag="ACG8XG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0774678.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         1576 a         1127 c          967 g         1750 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag
//
LOCUS       DAZKSR010000146         5339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000146 DAZKSR010000000
VERSION     DAZKSR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.151"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..414)
                     /locus_tag="ACG8XG_002807"
     CDS             complement(19..414)
                     /locus_tag="ACG8XG_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774679.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="ACG8XG_002808"
     CDS             complement(404..616)
                     /locus_tag="ACG8XG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0774680.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="ACG8XG_002809"
     CDS             1688..2290
                     /locus_tag="ACG8XG_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFQ0774681.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="ACG8XG_002810"
     CDS             2645..2881
                     /locus_tag="ACG8XG_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFQ0774682.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="ACG8XG_002811"
     CDS             2878..3291
                     /locus_tag="ACG8XG_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0774683.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3834..4760
                     /locus_tag="ACG8XG_002812"
     CDS             3834..4760
                     /locus_tag="ACG8XG_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFQ0774684.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="ACG8XG_002813"
     CDS             complement(4757..>5339)
                     /locus_tag="ACG8XG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774685.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1907 a          760 c          902 g         1770 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta ttgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DAZKSR010000147         5311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000147 DAZKSR010000000
VERSION     DAZKSR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.152"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8XG_002814"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8XG_002814"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0774686.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8XG_002815"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8XG_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774687.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8XG_002816"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8XG_002816"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0774688.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8XG_002817"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8XG_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774689.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8XG_002818"
     tRNA            3917..4000
                     /locus_tag="ACG8XG_002818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACG8XG_002819"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACG8XG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAZKSR010000148         5275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000148 DAZKSR010000000
VERSION     DAZKSR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.153"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..263
                     /locus_tag="ACG8XG_002820"
     CDS             <1..263
                     /locus_tag="ACG8XG_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774690.1"
                     /translation="KKKNPAQTSCLFFSNRFAHEINCFSHHRQFIPFTSFNLTATRHK
                     GVIATLSHFAYSSRLRSFVKVKTVVQGAVGIGVKACCSKFLD"
     gene            439..537
                     /locus_tag="ACG8XG_002821"
     CDS             439..537
                     /locus_tag="ACG8XG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0774691.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
     gene            complement(691..1188)
                     /locus_tag="ACG8XG_002822"
     CDS             complement(691..1188)
                     /locus_tag="ACG8XG_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774692.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRV
                     VFALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQ
                     ALKIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYF
                     ELNSQ"
     gene            complement(1216..1671)
                     /locus_tag="ACG8XG_002823"
     CDS             complement(1216..1671)
                     /locus_tag="ACG8XG_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774693.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGY
                     IKVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQ
                     VQKFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
     gene            complement(1679..2665)
                     /locus_tag="ACG8XG_002824"
     CDS             complement(1679..2665)
                     /locus_tag="ACG8XG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0774694.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLA
                     QTQLEATRRTFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPN
                     HEIYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQ
                     PKEKFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNK
                     GKRIGFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVT
                     NDDFSISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
     gene            complement(2650..3006)
                     /locus_tag="ACG8XG_002825"
     CDS             complement(2650..3006)
                     /locus_tag="ACG8XG_002825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0774695.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLC
                     KSVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQ
                     QLSQIEKGLIDLWEQL"
     gene            complement(3562..3927)
                     /locus_tag="ACG8XG_002826"
     CDS             complement(3562..3927)
                     /locus_tag="ACG8XG_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774696.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFM
                     RNASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLD
                     YKTFYPELLEFFQKLNSLE"
     gene            complement(3936..4097)
                     /locus_tag="ACG8XG_002827"
     CDS             complement(3936..4097)
                     /locus_tag="ACG8XG_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774697.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLT
                     EIIELDSKE"
     gene            complement(4087..4236)
                     /locus_tag="ACG8XG_002828"
     CDS             complement(4087..4236)
                     /locus_tag="ACG8XG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774698.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGE
                     QKNDG"
     gene            complement(4250..4999)
                     /locus_tag="ACG8XG_002829"
     CDS             complement(4250..4999)
                     /locus_tag="ACG8XG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0774699.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVL
                     DYCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVE
                     RPDGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGK
                     YALIMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELS
                     DKMSVSFFVTTLKRGRKVVGYEITINDTSKE"
BASE COUNT         1373 a         1195 c          976 g         1731 t
ORIGIN      
        1 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
       61 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
      121 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
      181 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
      241 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
      301 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
      361 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
      421 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
      481 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
      541 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
      601 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
      661 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
      721 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
      781 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
      841 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
      901 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
      961 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     1021 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     1081 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     1141 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     1201 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     1261 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     1321 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     1381 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     1441 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     1501 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     1561 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     1621 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     1681 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     1741 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     1801 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     1861 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     1921 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgccctcat
     1981 catcgaccca tttgaagcca atccttttgc ctttattgta ctcataaacc tcgacagcat
     2041 aggcttcttg aagatttttt ttgaaagaat caaaatcggt actcatatct tgtgcgccca
     2101 ggatcatatg cttcattttt tctcgattac tcattttgcc ctcggcgtgc atttcaaaat
     2161 caacttgttt gaatttttct ttcggctgtt caatgccaaa actcaataaa accttatcgt
     2221 ttaggtcttg taggtggtag ccgacctcgg tatcagagtg aaatttttta ccggtttcag
     2281 tgtcgatact ggcgaaaaca atgtgattat gaatatagcc ggattctgaa tcggtatgag
     2341 tgcccatgta gatttcatga ttgggatagc tttttgcgat ttcatcaacg agtttagcac
     2401 cgatctcatg cgctttttgc acgtccgccg gattgttttt gtcaaacgaa ttatcaaacg
     2461 attgaaccca atgaaaggct tgaataccgc ctgttttggc gaaagtccgt ctagtcgctt
     2521 ctagctgagt ttgagccaat ttattctcag ctgacagttt gtgcgtataa atgcggtcac
     2581 gatcaagctt tccatgccga tttacatagc caatcagcgc agaagcccca cgtttaccgc
     2641 cttttaaagc taaagttgct cccataagtc aatcaacccc ttttcgattt gcgaaagttg
     2701 ctggttcatc aaggctaaat ctgaacttga aggcatttgc cccttattca tgttaataaa
     2761 gcgagccatc tgattgagat ttccgccata tttgcgcaac tctttggcaa tttgtatcgc
     2821 cccctcccga tcaacgttcg gcgctcgata cttcatgcca agtgccacgc tcttgcagag
     2881 ctgtggcaca ctcataccag tttgatccgc aatggcggtc aaatactggt actcgtcttc
     2941 gctcactcga aatttaattt gccgactatc ttttcgattt tcggcattca atttctgctc
     3001 gctcatctca tcactcctca aataaaaatg ctccctattc ggtcgctttt ttcttctcgt
     3061 cattcttcgt ggtcgatatt caaagctaca aaaacgctcc ctattcagtc gccttttttc
     3121 cgcaatgaaa tctccctcgg ttatgccact acaaaatgaa agttccctac tcggtcactt
     3181 ccctttttcc gctctataac cgaatgattt attaacctca tcatactccc tattcggtcg
     3241 cataacaagg ttaataaatc atataatacg caagcaccgc atttggtacc caaatacatc
     3301 gcttgtcgag gtggcgttgc cccctacgaa ctcccccaat cacgcaacaa aaagtagcct
     3361 gttcggctat ttttattacg tgatttcgcc tcagaaaaag tagtctgttc gactattttt
     3421 ctactggctc atgcggtcaa acttgcacgc aagttgacct tatttttttg tctgcccgct
     3481 gttcgcaggc aaacaaaaaa gcaagccaaa aatcggcttg ctcgttcttc aaaaattgat
     3541 cgcatggact agctcgttcc gctattccaa gctgttcaat ttttggaaga actctagcaa
     3601 ctcaggataa aatgttttgt aatccaactt gtttccccag ttgtcttcca tccatttttc
     3661 gagttgcttt tgaaattgca ccgcttcggc tttcagctcg ctaggcaatt tttcggcaga
     3721 agtggccacc ggctttgcag ctgctggcgc aggtgcagat ccggcggacg acttcaatgc
     3781 ctttgacgca ttccgcatga aagtattgta ggcggaatag ctcttgaacc cttgtttttc
     3841 aaccatattt gtaccgattt tgactgaaag atcatgccga gcttcagcta attttttttc
     3901 caaatcttcg atatccattt ttgccatgcg taaactcact ccttagaatc tagttcgata
     3961 atttccgtta gaagaagatc aaattggttg atcgtttctt ctgaaagtcc accggtgtat
     4021 tcaataatag cgtcactatg cctttctaac agcttttcaa gctgttcaac gtcaatcgta
     4081 actgtcttag ccgtcatttt tttgttctcc tttttcgtca taactatgaa tcaaaaagcc
     4141 gtcacgttcg ccttttgcat agttcattag ttctttcata gcttgatcta gcgaagaatt
     4201 tttctttgcc atctttttac gcagtctttt tttcaactaa atcactcctc tattctttgc
     4261 tggtgtcatt gatcgtgatt tcatatccaa cgacttttcg cccacgcttt aaggttgtga
     4321 cgaaaaatga aactgacatc ttatcagata attcttcaat agcacgagta attacttttt
     4381 gaaaaaattg acctgcagga aggcgtttgt ctttaccaag aaaccaagat tcccattctt
     4441 ccaaatcacc tgtaatcgtt gtgtgttttt cttttcctct acggtaggat tcccagagtt
     4501 ttaacattat gagagcatat ttcccattga tccgagataa ttcacctaaa cgaaagctat
     4561 aaaacttttc tttgagatca agaacgtatg gagctgaatc agcagaaaat ttgaatctta
     4621 ctaacccgtc agcataaaaa tcaatccaag aaaataattg cgtcattctg agaccttgag
     4681 taccatcggg gcgctcgaca cgaagataaa gcgcagtatt ttcattaagc tttttgaaag
     4741 cttcagctat tcgacggtaa gaatctccag aggtgttcaa cccaaaatgt ttgataattt
     4801 ctaatgcggt tacctcgtat tcgtcatcta gttttgaatc tttagttaca aagctaaaac
     4861 agtagtctaa aactcgttgc tcaaacgatt taagccctcc aaatgcctta gctaagtcgt
     4921 ttccttgcac tactaaataa tcttgacggc ttagtaagtc ttttaatgca atatcagctt
     4981 ttttatttgg ttgtcccaaa ctgaaaatcc tccttttgta ctttttttaa aataatacca
     5041 tttattcagt ttttttgcaa tatttgaatt ttcaacgtgg aaaatagtat ttttgacgtg
     5101 gaaaatagta tttttgacgt ggaaaatagt attttcaacg tggaaaatag tatttatagg
     5161 cgctacaaac gctggtataa agccattcct tttcccctaa agtattttaa agtatttaaa
     5221 agtaatctat aaagtattct ctttaagcgc gcgggtgaga aaaaatttta aattc
//
LOCUS       DAZKSR010000149         5207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000149 DAZKSR010000000
VERSION     DAZKSR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.154"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACG8XG_002830"
     CDS             <1..1368
                     /locus_tag="ACG8XG_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774700.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..2989
                     /locus_tag="ACG8XG_002831"
     CDS             1355..2989
                     /locus_tag="ACG8XG_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0774701.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8XG_002832"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8XG_002832"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0774702.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="ACG8XG_002833"
     CDS             4077..5066
                     /locus_tag="ACG8XG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0774703.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DAZKSR010000150         5185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000150 DAZKSR010000000
VERSION     DAZKSR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.155"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACG8XG_002834"
     CDS             complement(<1..1097)
                     /locus_tag="ACG8XG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0774704.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACG8XG_002835"
     CDS             complement(1227..2603)
                     /locus_tag="ACG8XG_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0774705.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACG8XG_002836"
     CDS             complement(2603..3259)
                     /locus_tag="ACG8XG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774706.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="ACG8XG_002837"
     CDS             complement(3269..4546)
                     /locus_tag="ACG8XG_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0774707.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..4726)
                     /locus_tag="ACG8XG_002838"
     CDS             complement(4565..4726)
                     /locus_tag="ACG8XG_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0774708.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            4796..5185
                     /locus_tag="ACG8XG_002839"
                     /pseudo
     CDS             4796..5185
                     /locus_tag="ACG8XG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1661 a          947 c          654 g         1923 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccca
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattgt ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctttctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc ctgaatcggc tacactagac
     4741 tagacagaaa aaataaggtg tgtagaataa taaaaaacac actggaggat ttttcatgcc
     4801 aagacgtcaa cgaagaacct attcaaaaga attcaaacaa caaatcgtcg atctctatct
     4861 cgctggtaag ccttgcgcag gaattattcg agagtatgag cttacgcctt cttctttcga
     4921 taaatggatg aagcaagcac aatcaacggg ctcattcaaa gaaagagaca acttaacacc
     4981 agaacaagca gaattgatcg cactaagaaa gaaaaataag caactcgaaa tggagaatga
     5041 tattttaaag caagcggcgc tgatattcgg acgaaaagac aagtaattga tgccaacaag
     5101 cataaatatt ccatatcagc gatgtgcaaa attctaaata tttctcgtca aacctactat
     5161 tatcaagcga aaccgatcga aaatg
//
LOCUS       DAZKSR010000151         5025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000151 DAZKSR010000000
VERSION     DAZKSR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.156"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG8XG_002840"
     CDS             283..522
                     /locus_tag="ACG8XG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774709.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG8XG_002841"
     CDS             complement(884..1144)
                     /locus_tag="ACG8XG_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774710.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACG8XG_002842"
     CDS             complement(1329..1826)
                     /locus_tag="ACG8XG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0774711.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8XG_002843"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8XG_002843"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0774712.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8XG_002844"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8XG_002844"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0774713.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..>5025)
                     /locus_tag="ACG8XG_002845"
     CDS             complement(4548..>5025)
                     /locus_tag="ACG8XG_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0774714.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1043 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaa
//
LOCUS       DAZKSR010000152         4962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000152 DAZKSR010000000
VERSION     DAZKSR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.157"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..1816)
                     /locus_tag="ACG8XG_002846"
     CDS             complement(50..1816)
                     /locus_tag="ACG8XG_002846"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774715.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1776..3530)
                     /locus_tag="ACG8XG_002847"
     CDS             complement(1776..3530)
                     /locus_tag="ACG8XG_002847"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0774716.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3640..4209)
                     /locus_tag="ACG8XG_002848"
     CDS             complement(3640..4209)
                     /locus_tag="ACG8XG_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0774717.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4227..>4962)
                     /locus_tag="ACG8XG_002849"
     CDS             complement(4227..>4962)
                     /locus_tag="ACG8XG_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0774718.1"
                     /translation="KTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKD
                     INFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDY
                     QFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASL
                     SKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKN
                     GGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1644 a          940 c          668 g         1710 t
ORIGIN      
        1 aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat catcgtgatt
       61 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
      121 atatttctga ccttcgatac aactcactat gagtaccttg atcaactatt tttccttgat
      181 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
      241 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
      301 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
      361 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
      421 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa cgagctgctt
      481 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
      541 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
      601 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
      661 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
      721 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
      781 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccctctt
      841 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
      901 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
      961 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
     1021 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     1081 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     1141 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     1201 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
     1261 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
     1321 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
     1381 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
     1441 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
     1501 cgtctgcaat ttgcattctt aaatgcgcac tttctttata agtactatta tataagtttt
     1561 catactccct atctaacaac caatacatga aaagcaaagt aacgaacgat actgtaaaat
     1621 aaagccaagg cggctttaca ttttctaaaa ctaattcgtc tatcagcatc attataatga
     1681 atgctggtac catattaatg cagtatacaa aaaagctaga aatagatgct ttaaccaaat
     1741 cttctgcgcc ctgattagtt aaagcgaatt tgtttttata aaaacttttc attgtcaagc
     1801 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
     1861 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
     1921 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
     1981 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
     2041 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
     2101 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
     2161 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
     2221 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
     2281 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
     2341 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
     2401 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgttctaaa
     2461 actaagttga aattttctaa aacacactct tctttgtatg aaaagctgac attttcaaat
     2521 gcaatgtcac aatttttaat ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt
     2581 tctaaataaa gggagtctag tgtttcgact gcatatcttg cttcaaacaa gtgcatactt
     2641 acatacatta ttctcatgaa agaaacaaaa ataattccac ttaaaaaaaa caacatcaac
     2701 aagtcaacag ctaatatctt gggagtggta agagaagtta taaaaaaggt tataggaact
     2761 atcaaaatgc aaactatgcc aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca
     2821 ctcaaagaat acttatatgc aaacttagca taatcgttaa ttgctttatt caaggcttta
     2881 aatgatgtta ctttagcacc aaatatcttt ataacttgaa ttcctcggat atattctacc
     2941 gtttcagagc ttaacacatc taacgattct tgatattttt gaataaaatt tgtattctct
     3001 gtcattttct ttaacaagaa tacaccaatt actaacatgg cgattagaac caatccaatt
     3061 ttaaaattaa tgaaaaagcc taaggcaact gataaaacag gtgttactat ggcttgagca
     3121 ctatctggaa tcaagtgagc aattgccata tgtgttttag ccgcgttgtc atcaattgtc
     3181 tttcgaatat atcccgaaga atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg
     3241 ctaattccct tttttctcaa attagtctct aagcgaaacc caaggatatg agaaaataag
     3301 ccagaaaaaa tataaaataa agctcctaaa gttaaccaag caacagtttg aaccgcataa
     3361 ctcttagcta aatattcatc tcctacaata attagagact taagaaattt gtaaatactg
     3421 taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc taaccatccg
     3481 tagtatttca ttttagggac ataactaaat aattttttgt atactttcat tttttcctcc
     3541 cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg taatagcgta
     3601 aattagagat gtacaaacag atagaaggta aactacgttc tattttttgt atttttttaa
     3661 gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta acactgaaat
     3721 gattccccat atacctaccg catagtggat cattgcatcc attgttgcag cagattgtcc
     3781 tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa tccactgtcc
     3841 ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg ctcgatcata
     3901 tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac tgataaacca
     3961 aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt
     4021 aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc cagataataa
     4081 tccaaaaatc cctggggata tgctgtgacc aaacgcccca ccaatactac taaccattat
     4141 tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaataaaaa tataatcttt
     4201 tgttttcata tatattctcc tcaattctat ttcgttatgt cattaaaaat ctgttctatc
     4261 aaagttgaat cttctactct aagttttata tcttttttta ttccaccatt ttttaaataa
     4321 ataattctat tgcatgtttt ataaagaaat tccggatcat gagtggctat aactactgca
     4381 cccttttctt ttaagtcttt aataaggtca gcaatccttt ccatattaat ataatccaac
     4441 ccacttgtag gttcatcaaa tagaaataaa ttagtttttg acaatgaagc tatagaaacc
     4501 aataatcttt gtttttctcc accggataaa tcaaacggta attgatcttt cattttataa
     4561 agttccattt tttttaaaat tttttcttgc tgatctttac taactatttc attccccagg
     4621 gctaactcgc tcctcactga ttctgtgaaa aattggtaat ccatttcttg cattactaaa
     4681 aacggcgatg acaaattttt tttattttcg attatctttc ctttggtagg gcgaataatc
     4741 ttcatgagta ttcttagtaa ggttgttttc cccacaccgt ttttccctac caaaccaata
     4801 acatctccaa ctttgacatc aaaatttatg tcttttagaa tattttcata agtaatttct
     4861 tcaactttta atacatctgc acttaccttt tcgatatagg gaacttcaat cgaactatta
     4921 aaaatgtcaa atttccttaa tgcgttattg ttttcagttt tt
//
LOCUS       DAZKSR010000153         4931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000153 DAZKSR010000000
VERSION     DAZKSR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.158"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..970
                     /locus_tag="ACG8XG_002850"
     CDS             83..970
                     /locus_tag="ACG8XG_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0774719.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="ACG8XG_002851"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="ACG8XG_002851"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0774720.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="ACG8XG_002852"
     CDS             2272..2718
                     /locus_tag="ACG8XG_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0774721.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="ACG8XG_002853"
     CDS             2991..4343
                     /locus_tag="ACG8XG_002853"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0774722.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="ACG8XG_002854"
     CDS             4514..4801
                     /locus_tag="ACG8XG_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0774723.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DAZKSR010000154         4898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000154 DAZKSR010000000
VERSION     DAZKSR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.159"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..1734)
                     /locus_tag="ACG8XG_002855"
     CDS             complement(124..1734)
                     /locus_tag="ACG8XG_002855"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0774724.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1956..2201)
                     /locus_tag="ACG8XG_002856"
     CDS             complement(1956..2201)
                     /locus_tag="ACG8XG_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774725.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2927..4267)
                     /gene="glnA"
                     /locus_tag="ACG8XG_002857"
     CDS             complement(2927..4267)
                     /gene="glnA"
                     /locus_tag="ACG8XG_002857"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0774726.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4295..4687)
                     /locus_tag="ACG8XG_002858"
     CDS             complement(4295..4687)
                     /locus_tag="ACG8XG_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0774727.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1443 a         1053 c          799 g         1603 t
ORIGIN      
        1 aaaagtcttt tgctttctat cttttttcct acaaataata acaaatcaga aaaagagaaa
       61 agtgacaaga aaattgctta tccccttctc ttgctacttt tctctttcta tcaagttcaa
      121 aaattatttt gtttcgtgat ctgctaatga tgctccgaca aatgctttga ttaatttttg
      181 cgggcgattt ggacgagaaa tcaattctgg gtggaattgg catcccacaa agaatttttt
      241 ctctggcagc tctacgattt ctactaaacg gttatctggt gagacacctg aaaagactag
      301 cccttgttct tcaaacaatt gacgatattt gttattgaac tcatagcggt gacggtgacg
      361 ttcttgcaca acatcggctc catcatatgc ttcagccgtc ttcgttcctt ttttgatttt
      421 acatgggtat aatccaaggc gtaaagttcc acccaaattc tcgatgtttt cctgatcagc
      481 cattaaatca ataatattgt ttgctgtatc tggattcgtt tcagctgatg acgcatcttc
      541 taatccaacg acgttacgtg caaattcgac acaagccatc tgcataccaa gacaaatacc
      601 taaaaacgga acgtcatttt cgcgagcata acgaatcgct tcgattttcc cttctactcc
      661 gcgatcacca aatccaccag gaaccaagat tccatctgcg tctttcaaac gttcttctac
      721 attttctctc gttaattctt gtgctttgat ccaatcgatc tcgatgtctg aattataggc
      781 gaaaccagaa tgtttcaatg cttcaactac tgagatatat gcatctggta actcgacata
      841 tttaccgaca agcgcaatcc gtactttttt cttcaagttc aagacacgtt cttctaattc
      901 tttccattct gtcatgtctg cttctggtgt atctagtttt aaatggtcgc aaacaatttg
      961 atccattcct tgtgcttgta aaactaatgg gatggagtaa agtgtatcta catctggcga
     1021 ctcgataact gcttctggtt cgacatcaca aaattgtgcc aatttgtttt tcacgctttg
     1081 agaaacaggt tgctctgtac gtaccaccaa aatattcggt tggataccta aaccacgtag
     1141 ctcttttaca ctgtgctgcg ttggtttagt tttcatttct ccagcagctt tcaagtaagg
     1201 aatcaatgtt gtatggatat acataacatt atctgtaccg acatctgctt tcatttgacg
     1261 aagtgcttca aggaatggca aagattctat atcccctact gttcccccta cttctgtaat
     1321 gatcacatct gaatcagtca ttttagctgc tcgcatgatt ttttctttga tctcatttgt
     1381 aatatgagga ataacttgaa cagttgctcc caagtattcg cccttacgtt ctttacgcaa
     1441 aacttcagaa tagatttttc ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat
     1501 aaaacgttcg tagtggccca gatccaaatc tgtttcagca ccatcatctg ttacgaatac
     1561 ttctccgtgc tgatatgggc tcatcgtccc cggatccacg ttgatgtatg gatcaaattt
     1621 ttggatagtt acttttaagc cgcggttttt caataagcgt cctaaagaag ctgcaacaat
     1681 cccttttccg attgatgaaa cgacaccgcc ggtcacaaaa atatattttg tcataagttc
     1741 aaaaactcct ttttaattta tgtgcaggaa aagtctcaga caataaacaa agctccctat
     1801 tcttcaataa gaatagggag ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc
     1861 caaggtgtat aatacaagct gactaaggga aagtcaagta ctcaaatgaa tactatccgc
     1921 ttacaatttt ttctttttcg atatcctttg agaaattatt tcttaagtcc tttatgagtg
     1981 caaaaaaaat ttaacccaca taataagctt gatataaaca tagctaacgt taaacctaag
     2041 caaatcagaa aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca
     2101 aacattatga tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac
     2161 agtttatata gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac
     2221 gtaaaaaacg tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc
     2281 aaaaacaaaa caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct
     2341 tgtagggatt cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa
     2401 gtcgtgcctc cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc
     2461 acaatatcca tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa
     2521 atggtaggct cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc
     2581 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact
     2641 aaacgtcttt ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
     2701 agttcagcca attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt
     2761 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
     2821 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca
     2881 ggtcaaaacg ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta
     2941 gatattgttc tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt
     3001 ttgcttcaac aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct
     3061 tacgcaattc tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt
     3121 ctctttcttc ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct
     3181 gattacggat accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg
     3241 cagatggatc aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac
     3301 gtactaatgg cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg
     3361 gaacgagacg tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat
     3421 gtttcagcaa accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt
     3481 tatcatagaa aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac
     3541 cattgatccc aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa
     3601 tcgttttgac aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat
     3661 atttaaagtc aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa
     3721 agcctagact ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg
     3781 gtgcaaaatc aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt
     3841 ctaatttaaa taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt
     3901 cgcgcatatc tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc
     3961 catccggatt atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg
     4021 ggaagatcat ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa
     4081 caaatccttc aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct
     4141 ggctcactgg aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta
     4201 aaaatcgtac gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct
     4261 ttaccatggt atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc
     4321 cttgcgactg ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa
     4381 aaatttgtcg aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg
     4441 cctgtgccat ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt
     4501 ctttaatctc aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc
     4561 ttcgttccgg atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat
     4621 cagtcagctt cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt
     4681 ctcccatcag acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa
     4741 tattgtcaac tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac
     4801 aaaaaaataa gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt
     4861 tttattatac tgtactatcc tttattagtg tttttatt
//
LOCUS       DAZKSR010000155         4882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000155 DAZKSR010000000
VERSION     DAZKSR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.160"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            509..2353
                     /locus_tag="ACG8XG_002859"
     CDS             509..2353
                     /locus_tag="ACG8XG_002859"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0774728.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="ACG8XG_002860"
     CDS             2409..3875
                     /locus_tag="ACG8XG_002860"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0774729.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="ACG8XG_002861"
     CDS             3912..4754
                     /locus_tag="ACG8XG_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0774730.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          784 c         1028 g         1465 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa at
//
LOCUS       DAZKSR010000156         4871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000156 DAZKSR010000000
VERSION     DAZKSR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.161"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8XG_002862"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8XG_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0774731.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8XG_002863"
     CDS             724..1878
                     /locus_tag="ACG8XG_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0774732.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8XG_002864"
     CDS             2093..3061
                     /locus_tag="ACG8XG_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0774733.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8XG_002865"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8XG_002865"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0774734.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8XG_002866"
     CDS             4091..4699
                     /locus_tag="ACG8XG_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0774735.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="ACG8XG_002867"
     CDS             complement(4779..>4871)
                     /locus_tag="ACG8XG_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0774736.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DAZKSR010000157         4835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000157 DAZKSR010000000
VERSION     DAZKSR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.162"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..227)
                     /locus_tag="ACG8XG_002868"
     CDS             complement(78..227)
                     /locus_tag="ACG8XG_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774737.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1195..1521
                     /locus_tag="ACG8XG_002869"
     CDS             1195..1521
                     /locus_tag="ACG8XG_002869"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774738.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1529..2437
                     /locus_tag="ACG8XG_002870"
     CDS             1529..2437
                     /locus_tag="ACG8XG_002870"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0774739.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3063..3317
                     /locus_tag="ACG8XG_002871"
     CDS             3063..3317
                     /locus_tag="ACG8XG_002871"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0774740.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3859..4017
                     /locus_tag="ACG8XG_002872"
     CDS             3859..4017
                     /locus_tag="ACG8XG_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774741.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4089..>4835
                     /locus_tag="ACG8XG_002873"
     CDS             4089..>4835
                     /locus_tag="ACG8XG_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0774742.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDF"
BASE COUNT         1598 a          887 c          832 g         1518 t
ORIGIN      
        1 gaaaaacaac ctctttaaat ataaaaggta cgggttactg attacttgta aatctttacg
       61 ttccagtttt attctttcta aaaaatatac tcacttcttt tcttagactt ctttttttga
      121 ttctcctctt tatggtcttg tccttttttg ccttcaaaat atttaaataa acccctttca
      181 aaagcatttc taggttttgg acaataagga atgacttttt tcttcatata aatacctcat
      241 ttaactattt gatatcatca cattatcaaa aaaaataggt tttttattaa aactatcttg
      301 acctttcggg agatttcact gtgatttatt tttattccca tacttttttc gttggtcact
      361 ttcgtatacc taatctaagc gatcatcggc gacggtgctt ttacaacgaa tggcaggaca
      421 aaacaagggg cggaaaaact tttttgccta tttcataaaa aactttttcc tacagcaatc
      481 ccttgttttg tcctaccaca cgttgtgaac ccaaagcacg ccgacgaacg cttagctata
      541 cgaaaatgac ctccaaaaac aaaaaagtga ggtgaataga aacaaaaaga aattgaaatc
      601 tcccaaaatg tcgtccgcct cggagacaac ttgctgctga aaatggttgg ttaattcatt
      661 taagtattaa ataagataaa tcatcaaatt aatcggaaaa atagcatgat tggccttatc
      721 aaaaataaac tgtaaaaact tatatttcta tgagtggtgg aacaatgcta ataccaaatt
      781 aatttatcga ttacccacct gcacaaacca aagatagcat tattaggaaa cgaatcaatc
      841 agcgaatcca gcattataat gaggatacta aaaataaagt tttcataatt ataaatccgc
      901 cttcctacga aaggcaccaa tgaggtgatg aaaaccttag agcgctttct ttcttttttt
      961 attccgtgtt accctttcct tgcaggtccc tggtacacta cagaacccga ggaggtgagt
     1021 ggcggggctg tatagcggca accccgcata attatatggt gtcggtcatc cgttaaggca
     1081 tcaatgaatg gcgcaaaccc gtagcccgta aacttatctc ttccgaagtt gactcgattt
     1141 cgccggatga ccagtcccta ccaaacctgt actatttttt tacaggaggt tgctatgttc
     1201 aaaatctttc gtaaaaattg ttgtggcttg gatgtccaca aaacgtggat ctatgcgtgt
     1261 atcggaatca cagatgcgaa tggtcgtaca gtgtataagc aagctcgctt ctcttccttt
     1321 acaaaaggtc tcaatgaatt agctgaatgg cttgctaaat acaactgtaa ggatgtgtgt
     1381 ttggaatctt ccggtaaatt ttggattcct gtttttaata ttcttgaaaa aacttgtttt
     1441 gtcacactgg ctcatccgaa gtacaccaaa ccccagaaag gaaataaaac agatcgcaaa
     1501 gatgcaaaaa tggatttgtg atctctttat gtgcgacatg atcaaaccca gttttattcc
     1561 tggtcttgaa atccgccagc ttcgtgatct aatgcgttat cggacgaaac tgaccaacat
     1621 gaaaacaagt gaaaagaatc gtgctttaaa ctgcttaacc gtttctaatc tgaaactaga
     1681 tgatgttttt agtgatgtgt tcggaaaatc atccacttct atcatccaat acttgttaga
     1741 acatcccggt gaatcatttg atgttcgacc atttatccat gcccgttgta aaactcccgt
     1801 tgaagaaatc caagcggcag ttgagggggc gatttctgta cctcaagcga ttaagcttcg
     1861 ccaatgcctc aaccatatta acgagattga gaagcatcga gaagagatca atctagagat
     1921 tcaagctctt gcagaaccct tctcccctgt tttggaacta cttcgtacca taccgggctt
     1981 agacactcag ccaataacag cgattgcgat tctttcagaa ataggaccgg atatgtccgt
     2041 gtttccaact tcgaaacact ttatttcttg ggctggatgc tgtccaagaa acgatcgcag
     2101 tgcccgacga acaaaatcaa cacgtatctc tcgtgcaggg cagtcgttga aaccgctact
     2161 tgtacagatt gccaatgctc ttctcagatc caaagaacat ccagaattta aaaatcgcta
     2221 tcgtagaatc aaagcgaatc gtggacacaa gaaagctatc attgctattt gtaagatgct
     2281 cctgaccgct atctggaatg tcttatcaaa ggtggaaccc tattctgcta aaggatatct
     2341 ggagccaaaa cctgcgaaag aagagaaagt tctgacgcaa tcccaagctc tggacctttt
     2401 aagaaaacgc ggatatacca ttatagatag tgcttaaacc aaagttacgt tgattcttcc
     2461 taaatagact cgttgtcttc catttcaaaa attttgaaaa cggtaggctt atttagtgta
     2521 ccgttcttca ggtatcttca aatagattta ttttcaaact tatttccttt cttcttttac
     2581 tctcctatag cttagcattt aagtgggcac tcgtgtcacc ttgctagata gctatgaatg
     2641 ccgtaataag cctcctagta tataggcaga ctgccaatta taactgattc aatccacatt
     2701 atgataaaat ccaccttttc gtttcatatg tgttgaaata tcacatatgt caaaagtgca
     2761 cgagtctttt cattcagtgc tttgtaaata ctcaaaatta ttccttttca ctttaaaaaa
     2821 aagataatta gtacaaaatc agaaatgagg aaagtattag gatggactag aaaggagttg
     2881 ttgaatttgt tgcaacagat tggtcattta tcaattaaaa attaggtgaa gttttattca
     2941 tcactgcagc tgctgtttcc cagagaataa aacgtctaga agaaactggt cagttactgt
     3001 attatcaagc agcaattgac tacgaacaag cttgattact aaccaaagcg tttatccacc
     3061 tgttgattaa atcaaatcaa aaatcaggtt tttatacgtt cataaaagca atctccagtg
     3121 tactcgaatg tgactgtatc ataggtcctt attcgatgtt aatcaaaatg gggtttcctt
     3181 ctaccgaatc attggacagt tttatcaatg atttatcaaa gtttggacga accaacaccc
     3241 aaatcgtttt ctcgactctt gtttccttaa tggtttatca gttctggaat gtttatcatg
     3301 cgcttttatt aaaatagaaa agagcttttt tcatgcgaca aagcagtgat catttattgg
     3361 cacatgcaat ctttgtggat ccattttttg aaaatacgat atttagtttt gacccaaaaa
     3421 tctttaaata cgtatttgat aatttatctt aacaaccaga taattttatt cagcaccgta
     3481 ttatctatat tgaattctcg gaatcaagat aaattttttt ccgtttttgt ttctttttta
     3541 atcttcttct tgttattaat agtgattagg atgatattcc tatattgatc tttgataatt
     3601 agatataatt tttgaataac gttccctgct attcgagcta gaaatacaag tacgccatga
     3661 cctagttagc gacaactact tagagatttc ttttgttctg ggacaacaaa atgccatgac
     3721 aatagcagta aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt
     3781 cctatgtgct tacccaaagc ggttcaaaaa ttttaaacta ttattttagg agatgatttt
     3841 ataaaatcga aagcctactt gaataaaaaa caagcctgtg aatttctagg aattgataat
     3901 aatactttag ataagtggat tcgtgtatat aattttccag taataaaaat tgatggcgta
     3961 gttcgctttg acgtagagga tatcaaagat ttcatgaata cattcaaaaa aaattgaaat
     4021 tttcttctcg tcacatatac tataaatagc gaaatgtact gccttacttt ttaaaggagt
     4081 gaggaagttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg aggtatccaa
     4141 aaaaattcag agaaatcact ggaattgcat cagagaagta tcaaaaagta tttccaacta
     4201 ggcaattagc tattaaggcc gaaagggaaa tgaaaaagaa gattgaaaca gttttaagag
     4261 aggagaatgc aaactcacta gaattgaaag gaaaaataaa atttaaagat ttttatgaat
     4321 caaaatggct tcctagatat gaactaggtc aaactactag gtcaaacagg gctccaagta
     4381 atgtaacaat tagtaatact aaggatatat tccgattgca catccttcca atgtttggtg
     4441 aatatgcaat gaattatctg aattcaaata ctgagataat atcagatgaa ttaacaaaga
     4501 aatcgaaaga atacgcaaat ataaaaatta tcaaaggcta tgttcgctct atgtttgata
     4561 ttgccgaaat actatgttat atcgaattca accgaactac caaaattatt caatgtatta
     4621 ccgcgcctaa gaaaatggct ttagaagaaa aaaggataag ggaagggaat caagcattat
     4681 catcagaaga gctgactgat tggatagaag cagtaaacaa tgacttaaat aatcacttgc
     4741 ttacacttca cgattacaca cttttcatgc ttaccttata tctaggtgat agaaaaagtg
     4801 agacttatgc tctgcaatgg aaatatattg atttt
//
LOCUS       DAZKSR010000158         4812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000158 DAZKSR010000000
VERSION     DAZKSR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.163"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACG8XG_002874"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="ACG8XG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0774743.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1526..1721
                     /locus_tag="ACG8XG_002875"
                     /pseudo
     CDS             1526..1721
                     /locus_tag="ACG8XG_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3253..3771)
                     /locus_tag="ACG8XG_002876"
     CDS             complement(3253..3771)
                     /locus_tag="ACG8XG_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0774744.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4138..4386
                     /locus_tag="ACG8XG_002877"
     CDS             4138..4386
                     /locus_tag="ACG8XG_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774745.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1581 a          787 c          730 g         1714 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta gc
//
LOCUS       DAZKSR010000159         4796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000159 DAZKSR010000000
VERSION     DAZKSR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.165"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG8XG_002878"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG8XG_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0774746.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACG8XG_002879"
     CDS             complement(2066..3064)
                     /locus_tag="ACG8XG_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0774747.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACG8XG_002880"
     CDS             complement(3290..3943)
                     /locus_tag="ACG8XG_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0774748.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG8XG_002881"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG8XG_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0774749.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1406 a         1007 c          744 g         1639 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttt
//
LOCUS       DAZKSR010000160         4734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000160 DAZKSR010000000
VERSION     DAZKSR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.166"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      135..301
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            475..1581
                     /locus_tag="ACG8XG_002882"
     CDS             475..1581
                     /locus_tag="ACG8XG_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0774750.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1673..2428
                     /locus_tag="ACG8XG_002883"
     CDS             1673..2428
                     /locus_tag="ACG8XG_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0774751.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2486..4525
                     /locus_tag="ACG8XG_002884"
     CDS             2486..4525
                     /locus_tag="ACG8XG_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0774752.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGATVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGFDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT         1568 a          803 c          997 g         1366 t
ORIGIN      
        1 aacgggattc tttcaactta tttgttattt ttttctaaaa aatattaaag attgttaaat
       61 ttcactattt catttcaaat tccattgcct aatcattagg aaaagtctat catgaactcg
      121 taaatagaat atagtaataa ccttgttagg tgaggctcct atacaaacat aggctgttgc
      181 ccagaaacgt cgaaagtcgc caatgggtaa aacagaaact gttggattaa ggctttttcc
      241 aatagctaag aatgttttct ttacgttgta tagtgctaaa actcggacga tgagatgatg
      301 ctaggtcaga attcttgttc gaaaaggttt ctgacctttt ctttatattt ttgtgactga
      361 cttatttgat gagacaaact cgctcctcac ctgacaagtg gattgctgtg tgaggaggag
      421 atgcggtgaa aaaagatcga ttggtggatt ttgggtcctt atttgacttt gtccatgatc
      481 ggtcttttag aagtatacag tgccagttct tatcgtttat tgcaagccga tgaaaataca
      541 aaaagtctat tattgcgtca actaattttc atatttttga gttggagcgt gatcttctta
      601 gctcgttcag tcaaactaca ctatttactt caccctaaaa tagcaggata cggtttagcc
      661 ttatcgattt tctttttagt attagtaaga atagggatat tcggtgtcac tgtcaacggc
      721 gcacaacgtt ggatctctct gtttggcatt caattccagc cttctgaact ggcaaatctt
      781 tttttgattt tttatttaag ctggtttttt cgtgacggaa atagtagccc aaaagatcta
      841 aaaaaaccat tcctgattac agtaggtata acttttctga ttttatttca gccaaagatt
      901 gctggagcat tgatgatcct ttcgattgcg tgggtcatat tttgggcagc ggcggttcca
      961 tttaaaaaag ggatctatct aatcgttact ttttctgcat tgctgattgg agcagcaggc
     1021 ggggtattat atttaggaaa taaaggttgg cttccacaga tgtttaatca tgcttatgaa
     1081 aggatcgcga ctttaagaga ttcttttatc gatagtcatg gtgcgggcta tcaaatgacc
     1141 cattcgtttt atgcactata taatggtggc atatttgggc ggggattagg aaatagtatc
     1201 acaaaaaaag ggtatcttcc agagacagaa acagatttta ttttttcgat tattacagaa
     1261 gagcttgggc taatcggtgc attgtgtgtg cttttcttat tgttctcatt atgcatgaga
     1321 atcttttgtt taagcagtcg ctgtaaaaat cagcaagctg gattgtttct tttaggattc
     1381 ggaactttgc tattcgttca gacgataatg aacgtgggaa gtatcgcggg gctgatgcca
     1441 atgactggtg tgccattgcc atttgttagc tacggaggaa caagttatct gatcctttct
     1501 ttaggaatcg gaatcacgtt gaatatttct tcaaagatac aggccgaaga gttggcgctc
     1561 tatcgacctg aaaaacaata aagagaaact gagaaactct cagtttgaat tatcataaag
     1621 tacaatcttt tatttttcaa gcgagaggtt cttttagaaa gaggaattcg caatgaaagt
     1681 atctcaaaaa atcatattat ctattttgtt actgcttaca ttagtagttg gctatttttc
     1741 gattgagttt atccatggat tttcgtctgc aaaacagagt tcaactgtga aaaaggtaga
     1801 gccgaaaagt atccctacca cactaaatgt ggctttgatt ggttcagatg ctcgttcgaa
     1861 agacgaaaac ggtcgctcgg attcacttct ggttgcacaa tacaaccaga aaacacaaca
     1921 agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt acggaatgaa
     1981 taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa cattaaagga
     2041 aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt ttatcgattg
     2101 cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc tagatttaga
     2161 tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac tccagtatgc
     2221 tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc agcaagtgat
     2281 caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac ccaaagcggt
     2341 tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg
     2401 tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga aataaaggaa
     2461 tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac
     2521 aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg gttattttta
     2581 ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag ttgagcaatt
     2641 tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat caaaaaaggc
     2701 ggcaaataaa agtgcattat ctgaaaaaga gatcttagat aaataccaga atatatacgg
     2761 tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa aagacgattc
     2821 tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat tgaaagatct
     2881 ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact ggcaacccaa
     2941 cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac aagaagcagc
     3001 aagagggaat attatagatc gaaatggtga accattagca acaacaggca aactaaaaca
     3061 attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca atatcaaagc
     3121 gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt cacaaagctg
     3181 ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc cagaacttcc
     3241 agctggagct accattcaag aagtagacgg cagatattat cctttgggtg aagcagctgc
     3301 tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga atccagaatt
     3361 aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata aggatcttcg
     3421 tgggacaaca ggtgggaaat taagcattac agatgcagac ggtgtagaga aaaaagttct
     3481 gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg cgaaggcaca
     3541 aaaaacagct tttgacagtc taggaggaaa agctggagca actgttgcga caacgccaaa
     3601 aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca aaatgacaaa
     3661 cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacatc cattcatcag
     3721 ccgatttgcg acaggttatg ctcctggatc tacgtttaaa ataattacag cagcaatcgg
     3781 tttcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc ttaaatggca
     3841 aaaagacagt tcttggggat cttttcaaac aacgcgtgtc agtagtgatg tatcacaagt
     3901 agacttaaaa actgctttga tctattccga taatatatat gcggcacaag aaacgttgaa
     3961 aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg aagaccttga
     4021 tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta actcagatat
     4081 cttgctagct gatactggat atggacaggg cgaacttcta attactccta tccagcaagc
     4141 agcaatgtat tctgttttta ccaacaatgg cacacttgtc tatcctaaat tgattgcaga
     4201 taaagagaca aaagataaga agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc
     4261 agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt ctgctttagg
     4321 gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg
     4381 gcaagagaac agtttcttgt ttgctttcaa tcttgataac caaggttata tgatggtcag
     4441 catgttggaa aataaagaag atgatgattc agcaaccaaa cgagcatcgg aactattgca
     4501 atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa aggactgtgg
     4561 cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct ttcacaactt
     4621 ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga acaagtggag
     4681 aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt ggtt
//
LOCUS       DAZKSR010000161         4630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000161 DAZKSR010000000
VERSION     DAZKSR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.167"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8XG_002885"
     CDS             complement(52..522)
                     /locus_tag="ACG8XG_002885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0774753.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8XG_002886"
     CDS             complement(681..1139)
                     /locus_tag="ACG8XG_002886"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0774754.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8XG_002887"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8XG_002887"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0774755.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8XG_002888"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8XG_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0774756.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8XG_002889"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8XG_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0774757.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1452 a          845 c          876 g         1457 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa
//
LOCUS       DAZKSR010000162         4592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000162 DAZKSR010000000
VERSION     DAZKSR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.168"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..2858
                     /locus_tag="ACG8XG_002890"
     CDS             189..2858
                     /locus_tag="ACG8XG_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0774758.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3563)
                     /locus_tag="ACG8XG_002891"
     CDS             complement(2949..3563)
                     /locus_tag="ACG8XG_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0774759.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3682..4221
                     /locus_tag="ACG8XG_002892"
     CDS             3682..4221
                     /locus_tag="ACG8XG_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774760.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4218..4556
                     /locus_tag="ACG8XG_002893"
     CDS             4218..4556
                     /locus_tag="ACG8XG_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFQ0774761.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1422 a          790 c          860 g         1520 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaataacgg
     3601 tgtacatttt taaaaggaga tgtataatcg agatttagaa agcgttttcc tcaaaattta
     3661 atgaattagg aagtgattac tatgaaaaaa ttatttagcg cactttttat tctgttattt
     3721 ggctttattg gtattgggac ttctggaaat atagttaaag ctagcacgat ggttgaacaa
     3781 acaagtgaaa tggcaaaaac ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa
     3841 tccaaaggat ttagtgataa agatactttt tacactcaaa agatagagcc tcaagatccg
     3901 aaaacacgtt tttctgcaaa tgtattgcgg ttaaccgcat ctactaaaaa aattactaat
     3961 acgcaggggc ggacagaata tattataaca agtcccaatt cggcgatgta tcgtatttct
     4021 acacgcttga gtttaggtag tgcaaaatca tatactcaaa cagtaaatct agctaattct
     4081 tatggttatg gtggaggaat gtattttaca tttactggga aaagacaata ttacagtgta
     4141 aaattagcta tgcaagttta taatacattt ggtgtagggt cagtctcttg cactgcggga
     4201 ggattgacaa taggaaaatg ataatttctt actatgtctt aaatagaagt gatgatgaag
     4261 gactcttcgt ttttcaatca ttacatgagg atacgatgct aaaagagttt tataatcgga
     4321 ttaagaataa acagatagaa acaaatttat attttttaag tgactctatc ttgcctttta
     4381 ttagcaaccc taaaattctt aacttgttgc agacaaaaga tttgaaaagt ttaccgatta
     4441 ttgtcattga tgaggaaatt gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa
     4501 aattaactaa cattggaatg agtattcagg aggataatgg ggtacatgga acatagaatg
     4561 aatatgcaca gttgagaacg gaatttgaca tt
//
LOCUS       DAZKSR010000163         4548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000163 DAZKSR010000000
VERSION     DAZKSR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.169"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1152
                     /locus_tag="ACG8XG_002894"
     CDS             82..1152
                     /locus_tag="ACG8XG_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0774762.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            complement(1118..1204)
                     /locus_tag="ACG8XG_002895"
                     /pseudo
     CDS             complement(1118..1204)
                     /locus_tag="ACG8XG_002895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1248..1709)
                     /locus_tag="ACG8XG_002896"
     CDS             complement(1248..1709)
                     /locus_tag="ACG8XG_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFQ0774763.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(1969..2517)
                     /locus_tag="ACG8XG_002897"
     CDS             complement(1969..2517)
                     /locus_tag="ACG8XG_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774764.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2518..3375)
                     /locus_tag="ACG8XG_002898"
     CDS             complement(2518..3375)
                     /locus_tag="ACG8XG_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0774765.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3661..3948)
                     /locus_tag="ACG8XG_002899"
     CDS             complement(3661..3948)
                     /locus_tag="ACG8XG_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0774766.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3938..4267)
                     /locus_tag="ACG8XG_002900"
     CDS             complement(3938..4267)
                     /locus_tag="ACG8XG_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0774767.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4344..4490)
                     /locus_tag="ACG8XG_002901"
                     /pseudo
     CDS             complement(4344..4490)
                     /locus_tag="ACG8XG_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         1379 a          877 c          658 g         1634 t
ORIGIN      
        1 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
       61 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      121 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      181 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      241 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      301 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      361 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      421 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      481 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      541 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      601 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      661 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      721 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      781 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      841 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
      901 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
      961 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1021 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1081 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1141 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1201 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1261 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1321 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1381 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1441 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1501 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1561 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1621 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1681 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1741 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt attaatgaaa
     1801 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
     1861 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
     1921 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
     1981 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
     2041 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
     2101 caagtctagt tctttcacca cgtcattaaa caaatcttgt ttatcttctt gcgtaagatt
     2161 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
     2221 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
     2281 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     2341 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     2401 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     2461 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     2521 ctccacctcc acataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     2581 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     2641 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     2701 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     2761 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     2821 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     2881 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     2941 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     3001 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     3061 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     3121 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     3181 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     3241 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     3301 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     3361 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     3421 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     3481 taagtaaaag gtagtacata aataaggaaa agctgaaata tattgattta tagctttcct
     3541 caaagtaatg acgttattta atttatcgag aagcagagaa tactcgcgac tttagtcatg
     3601 tagacgaatc agattttcga taaaaaagaa gccgtccttc ttaagaacga caacaaatca
     3661 ctagattaat tttttcaact gttcataaaa gttttcatgc gttccacata acaatactgg
     3721 aataaccttg ttttctataa tctcgtaagc tactctatat gttgtgcccg catatttgaa
     3781 cccactagac caaatacctg ataaatcccc agttttttgt tcacccttat atggatcaat
     3841 cgcaatttca tcataaatta aattcaaaaa tttttctttc aatggcttct cttttagctt
     3901 ttttaaatac ttaatgacat gttttgaagg aacgatttca taagccaatt tctgttgcca
     3961 actcctcttt agtcattact ttagaattag taagtgtatc ttcagcaata tctctaaagg
     4021 aagaatgcat tttgtttttt ctattcttaa attctacaag aatctcatct ttatcgtagc
     4081 cttctgcaat tatatcagac aaaatatctt cgctaaaatc ataaaattct ttttgtggtt
     4141 caacaaattc ataaagaata ccattctcta ccaactttgc ttcaacttcg actccattag
     4201 gaacattaaa atctttaggt actgtcaaca tgattgaatt gccttgtttt cttgtttttg
     4261 taatcattta aatccctcat ttctaacagt atgtaattac ttagtaaata cattatacca
     4321 cgagataatg tatttacaag taattactgt gactcgtaat gcgtccttgc tgaataaatt
     4381 tcaaccacgt gttcatcttc atctacctta taaacaaccc ggtgttgtct attcaatctt
     4441 ctaaaatacc taccttcatg tgtaggtttt aacttctcaa atgattgggt tctgttgcaa
     4501 agttttaaat aaagaataaa atcccttacg gtatctatga tttaagct
//
LOCUS       DAZKSR010000164         4496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000164 DAZKSR010000000
VERSION     DAZKSR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.170"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(599..1246)
                     /locus_tag="ACG8XG_002902"
     CDS             complement(599..1246)
                     /locus_tag="ACG8XG_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0774768.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1376..2314)
                     /locus_tag="ACG8XG_002903"
     CDS             complement(1376..2314)
                     /locus_tag="ACG8XG_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0774769.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2805..4022
                     /locus_tag="ACG8XG_002904"
                     /pseudo
     CDS             2805..4022
                     /locus_tag="ACG8XG_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            4156..4395
                     /locus_tag="ACG8XG_002905"
     CDS             4156..4395
                     /locus_tag="ACG8XG_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0774770.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT         1628 a          717 c          679 g         1472 t
ORIGIN      
        1 tttttgctta ttttttaaat tattttggga aattttccca atcccttttt ctaactcaaa
       61 aaatataatc actcaaaatt taaaagggcg cacttataca tcattttaaa aaattgatgt
      121 aacgtgctaa gttcaaaaca aagggcgcac ttatacacga ttttcaatct tgtatatttc
      181 taacgaaaag cgtgcgccaa aaaaccccct tcgtcaattt tgacaggggg ctttttgatg
      241 taaaaatttc tatcgaaatt taaaaattcg cttcactcat gttataaaga cttaaaataa
      301 aataactctt taaaatcttt tgctagttgt tcttcaatat tttttattcg gtgcatcttc
      361 caagtaaagt ataacacact agacttattt actacgtttc ataagtcatt aatgcgtgtg
      421 ctctgcgagg ctagtttttg tgcaagcaca aaaaatggac tgaataaatc agtccataag
      481 ttcaaaacca aattcaaaat caaaaccaca agcaaccaaa aaatgtggtt gttatacgtt
      541 cataaatttt atgatcactt acgtgtataa aattaaattc actttcaaaa tctaaaaact
      601 aaatccaatc atctacccta tgaattatat cttgaaattc attcataaat agtgaagcat
      661 ggtaaccatc acatacagaa tgatgaagtt gcagagcaac tggtatataa attttattat
      721 tctcactata aaatttacct atcgtaataa taggcaataa aaagctgcta ttgttaccaa
      781 tatttaaatt aaatgaacta aaatcaatcc aaggaatcat tgaaatcggt atggtgtttt
      841 caggtatcgg ttttttagga aacatttctt ctttatcttt atattcaagc aagtcatttt
      901 tataattatt ataaaaagaa atgaagtttt tatcagattc agtccaaatg ttagtaaatt
      961 tttcagtttg cttattaaaa actgtataca aaggatttaa cttatcccaa taacctaatt
     1021 tattctcact attaattcct gttctaaaca ctttattttt atttacaact tccataattg
     1081 cataaattaa agagggataa atttcatatc ctttcttttt tatcatatct ttaaacaaag
     1141 taatatcaat ttctttagta atgctataag tagtttgctg attaaaatag tgttcaaaat
     1201 attcttttct atcccaattt tctaattcaa taatattaaa agtcatatat aacttcctcc
     1261 taaattttaa atttttatat ttaggaggaa taatcctctg attttttcat acgttatgtc
     1321 acctcgtaaa tattaattat actgaattag caatttttat caaataaaac ttattttact
     1381 tccaaaacct aaattcacgt tgccaaaaat caatctgctt ttgcaattgt ttttcgttcg
     1441 cttttaaagt cgatttcatt aattccgtta aatcaattgg agatatttct ctaatcaatt
     1501 ttttaaattt agtcttagta ttcttactta gctttcccca catactttct tcatgcaaca
     1561 aagtataaac catagcttgc tcattaattt tttctaaagt agcccacgca ggtttcaaga
     1621 tgtgtaaatc attaaaacaa tcattccagt aatcaaccat atctcttttt aattcaactt
     1681 ctacacgcca taaatgttca gacacaactt caacatctgc gttatcttta cgttcttgtt
     1741 ttttattata aattctaata aatctatcac tatcacggac accaaaatat tttgtttctg
     1801 gcttgccatt acgaccataa aaaacagttt tcttaactgc tttatcagtc attgcatagt
     1861 aatcgctcaa atcatcttca aaatcaaaag ctaagtctaa tcttgtaaaa ccgtcatctt
     1921 ccatgtagtc gataatattt tgttttaacc aaatcatttc ttcatgtgtg agtttattgg
     1981 gattaaattc aacacgcata ttacgtctat cccaagtatc tgcttttact ttgtcatatt
     2041 cgatataaac tttttcttga agtgccttag ctttaaactt tgtttgaagt atatcccaaa
     2101 gtcgtatttg tggctctaca ctcataaagt cagatagctt tttagcatta gttttgttca
     2161 aatttccaac gattgtcatg gcatcaaaac ttaatgcggg ttgagatttt cccaaagttt
     2221 gaccacttaa ccggctatta cttaaccggc tattagagac ggccggtttt tttagtgaga
     2281 tttctgcagc taatttttca tcaattttct tcatttttta tcgctcctaa aataattttt
     2341 ggaacgaaaa aaggacgagt ggaatcgcaa aataaatttc gcctgtatcc attcgtccat
     2401 tttgttttgt attcaaaatc aaaaattgat atactaatca tcgaatgaat aacaagtgtg
     2461 gtttgttatt ccgggcgaga acgttggtag cgttgctcgt ctttttttat gtcttttttt
     2521 cgttaaaact attttaaaac taaatatatc ttaaagcaaa taaattcaag gggctaaaag
     2581 cgccccttaa aaaccccttg cctctgaaaa gttttgtgtt tcctttccgt cggtttcaaa
     2641 agctaattta ctcaaaaaaa tagatcaagg gtaaatgcac tcactgcgtt cgcccttgag
     2701 ctattttatt tcgcaaattt taacgcattt gccgacgaaa agataaaaca cataaaaata
     2761 tacgcagata tatttaaaac gtcttagaac gcaaaatttt acgtttaaaa gctatgcaag
     2821 gactgtatga agcaaaatta ttgagttatc caagaacaga tacaccattt attacagaga
     2881 acgaatttgc ttatttaaaa gcgaattttg gcaaatatag cggtttttta ggacttgatc
     2941 ttgaaatggt tcaaacagag cctagaaagc gttatgtgga cggtagtaag gtacaggaac
     3001 accacgccat tatcccaaca aaacaagtac ctaccgaatc tgcattagcg aaaatggacg
     3061 atttacaacg aaaaatttat gctttagtcg ttaaaacgac cgttgccatg tttttacctg
     3121 attacttgta tgaagaaacc aagatacaaa cgaaagtagc tgatttgctg tttcaatcga
     3181 tcggaaagac accaaagcaa gaagggtgga aaattctttt caaacaacaa accaaagaag
     3241 aaaaagagga cgttcaaacg ttaccactcg ttatcattgg cgaacgtgcc gaagttggtg
     3301 ttaagagtgt tgaaaaagaa acgcaaccgc caaaagcttt tacagagggt acattattaa
     3361 ctgctatgaa aacggcgaat aaaacggttg atgatgaaga agcaatcaag attttacaag
     3421 aagttgaggg gattggaaca gaagcgacaa gagcaagtat tattgaagcg ttaaaacaaa
     3481 aagaatatat ccaagtgatt aagaataagc ttgttgtaac tgaaaaagga aaattattgt
     3541 gccaggcagt tgaaagtcag caccttttaa cgagtgctga aatgacggct aaatgggaaa
     3601 cgtatttaaa aaaaatcggt aaaagagaag gcaatcaaga gaactttatt acgaatatca
     3661 aaaaattcat tgttcattta ctggaagctg tacctaacga tatagaaaaa ctaaattttt
     3721 ctgattacca ggaacagaaa gaaaaagaag cagaaaaaag tattgtagga aaatgtccta
     3781 agtgtggcaa caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat
     3841 gtaagtttac tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag
     3901 aattactgga gggaaaagaa accctggtaa aaggaatcaa aaacaaagag aaaaagccct
     3961 acaatgccgt tgtaaaaatt ggggaaaagg gatatattga ttttatatct ttctcaaaat
     4021 aaacataaaa gccctttaaa gagggctttt atatattaat cacaaatcac ttatcacaaa
     4081 tcacttatca caaatcacaa gtgatttgtg attgttgatg ataaaataag aataagaaga
     4141 aatagaaaga agtgagtgat tgtgggaaat ttaggcgcac aaaaagaaaa acgaaatgat
     4201 acaccaatca gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac
     4261 ttgcaccata tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg
     4321 gaaataagac ttagaagcaa acttaagagt gtgttgatag tgcattatct taaaattttg
     4381 tataatagga attgaagtta aattagatgc taaaaatttg taattaagaa ggagggattc
     4441 gtcatgttgg tattccaaat gcgtaatgta gataaaacat ctactgtttt gaaaca
//
LOCUS       DAZKSR010000165         4477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000165 DAZKSR010000000
VERSION     DAZKSR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.171"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            598..744
                     /locus_tag="ACG8XG_002906"
                     /pseudo
     CDS             598..744
                     /locus_tag="ACG8XG_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(832..1125)
                     /locus_tag="ACG8XG_002907"
     CDS             complement(832..1125)
                     /locus_tag="ACG8XG_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0774771.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1162..1347
                     /locus_tag="ACG8XG_002908"
     CDS             1162..1347
                     /locus_tag="ACG8XG_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774772.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1528..2706)
                     /locus_tag="ACG8XG_002909"
     CDS             complement(1528..2706)
                     /locus_tag="ACG8XG_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774773.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="ACG8XG_002910"
     CDS             complement(2810..3124)
                     /locus_tag="ACG8XG_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774774.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="ACG8XG_002911"
     CDS             3231..3446
                     /locus_tag="ACG8XG_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774775.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="ACG8XG_002912"
     CDS             complement(3746..3988)
                     /locus_tag="ACG8XG_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774776.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="ACG8XG_002913"
     CDS             complement(3975..4403)
                     /locus_tag="ACG8XG_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0774777.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DAZKSR010000166         4458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000166 DAZKSR010000000
VERSION     DAZKSR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.172"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..885)
                     /locus_tag="ACG8XG_002914"
     CDS             complement(460..885)
                     /locus_tag="ACG8XG_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774778.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1259..2230
                     /locus_tag="ACG8XG_002915"
     CDS             1259..2230
                     /locus_tag="ACG8XG_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0774779.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2262..3659
                     /locus_tag="ACG8XG_002916"
     CDS             2262..3659
                     /locus_tag="ACG8XG_002916"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0774780.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3818..4441
                     /locus_tag="ACG8XG_002917"
     CDS             3818..4441
                     /locus_tag="ACG8XG_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774781.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1561 a          715 c          909 g         1273 t
ORIGIN      
        1 aaattactac gtgatgtaga ataagccttt ctatcgtttg gttattttat attcaaatat
       61 ttgaaataaa taaatcttta ctctttgatt tgtaaactga aatgatccac tgtttgtttt
      121 actgatgaag cttgatcaaa ggaagaagct gagtttgaag ttcataaaaa tcagccatag
      181 tgagttttgc ttggatttgt ttatcttctt ctacttttta cataattttg aacaggataa
      241 gattcaaatg ttttaaatga atctttgact agtttgtgac agttgtcgca tatgctcaag
      301 cttccttggt cattaaattc tgtatggata tttttgttgt tattacatgt acaataatca
      361 ggactatcga tcatccattt cgtctccttt acaatcatct tgcttcttta ctatagcaga
      421 aaagaagggg agaaacaaaa gaagatccaa cagacggcac tatagactgt agatgttgcc
      481 atcctcatcc ttcaaacggt aattaccgat ttttgtaaat atgactgttg tttctttttt
      541 attttctttt cctgaaataa tatattcgtc atctttggta gtgatcttga tatctgtcag
      601 cactgttttt tcttcagatg attcagaaga atcagttcct tgtgtctgat acgtgcgtgt
      661 ccattccatt tttttatctg taaatgtcaa ctgtcccgcg gataaatcca tatctttacg
      721 tacttttagc gtatatacac tgttggtggt atctgctttt tccgctttta agacagaaga
      781 atcggttgtt tcgtccgagg attggtcaga gccgccaaac aaatcacagc cagtcaatgt
      841 caaaagtccc gcagacaaaa taaatagtac gattaatttc ttcaaaatac ttcccccatt
      901 tgataggata aagaaattat accattggaa tgtaaaactt caaacggatg gaagagaaaa
      961 acgcacaaaa cacccacaaa tagagtaaat tttagaataa aattttaaaa aggcaaaaaa
     1021 tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat
     1081 taaaaatgaa ccggttcaaa tacttgttta agtataatta atagtgagag gaagttgctc
     1141 tactgaagac tttagttata tggatattga tgacaagcaa cactatgcaa agaatgcggt
     1201 catcataatc gaacaacaag aaagagaaat caaatgtaac tggcaggagt gagaatgtat
     1261 ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag tttcccgtta
     1321 cttgaatggt cagcaactga aggaaaagaa catgaaaaaa atagcagaag cgatttcagc
     1381 gttggactat aaagaaaaca tcattgctaa agggttgaag aataatcgaa gcttctctat
     1441 tggacttttg ataaacggga tttccagccg ttttgggtcg gaagttgtct cagggatcga
     1501 aagagtagct gaagaaaatg ggtatagctt attattaagc gggtttgccg atcagccaga
     1561 aaaaattgaa caaaaaattg aatacctgat gaagcatgtg atcgatgggc tgattgtctt
     1621 cttatccgaa gaagagtggt ctggatttga aatgctcaca aaaatgagta ttccagttat
     1681 cgcattgaat tgtccagaag gaccagcagg tattgataca atattagtga atgatcgaga
     1741 aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa tcggatttat
     1801 tgctgccact caaacagact acgtagcaag agaacgttta gcaggagcca aagaagcggt
     1861 ccaaaatgat ccatcgatcc aattagaggt ctttacagga gactattcta gaaaaagcgg
     1921 ctattacggg gcaaaagcat tattagaaaa aggggcaact gctatttttg tcagcaatta
     1981 caatatgtcg attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga
     2041 tattgcgttc agtcactatg attatttaga taaaaacgat ctgagtatcc ttccgaaaat
     2101 cacgatcacg ccaccaacag ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg
     2161 aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg atggaaaata tcatacatgg
     2221 aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc
     2281 cagcaaattt ttggtggggt tctgcagcaa gtggcccaca aacagaagga aggatttcag
     2341 gcgatggaaa aggagagaac atttttgatt attggtacaa aaaagaaccg caaaagttct
     2401 ttgatcaagt aggacctgaa aaaacctcgc aagtttatac gaagtatcaa gaggatgtac
     2461 agttgatgaa acagactggg cataattcat ttcgaacatc tatccaatgg agccgtttga
     2521 tacctgatga atctggaaca atcaacgaag aagccgttcg tttctatcat cgttattttg
     2581 atgagttgat tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc gatatgccga
     2641 tgtatctgca agaaaaaggc ggatggttga accgagaaac ggttgaagcc tatgaaaaat
     2701 atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt actcataatg
     2761 aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg gcattatcca gaagagtcag
     2821 atatgaaaaa agcgatccaa gttggctatc atgaagcgtt ggcttcagcg ttagccatca
     2881 aatcttacca tgaaatggag caaggtggtg ttattggtat catcttgaat ctgacaccaa
     2941 gctatccaag agacgaatca aaccctgaag atgttaaagc agcgcaaata gcagatgcgt
     3001 tcttcaatcg ttcattcctt gatccagctg taaaaggcga gtttccaagt gaattgattg
     3061 agatagtaaa agaacttgat ctcatgcctg tgatccatgc agaggatttg acggttatca
     3121 aagaaaatac agtagatctt ttaggtatca attattacca gccacgccgg gtaaaagcaa
     3181 aagaaacacc aatcgatact aaaaacggtc caatgcctga ggattatttc gacaattatg
     3241 aaatgcctgg tcgtaaaatg aatccttatc gcggatggga gatctatgaa caaggaatct
     3301 atgatatttt gatcaatgta cgagatcatt atggaaatat cgactgcttt atttctgaaa
     3361 acggtatggg tgtagaaaac gaagcacgat ttatcaaaga agatggcatg atccacgatg
     3421 attatcggat cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc attcaagaag
     3481 gtgccaattg tttaggctat catatgtgga catgtatgga taactggtcg tggaccaatg
     3541 catataagaa tcgatatggc tttatttcag tagacttagc acaagatgga aaaagaacaa
     3601 tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa aaacggattt gaggcttaag
     3661 aaagaatagt tgagagcaaa tgcgtgaaat tctccattct taccattttg tgaaaaagaa
     3721 gttcaattat ttacgatctt tttcaacttt tttgtctttc tatttcattt tttgtatata
     3781 atagaatagt caaaattaaa taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct
     3841 tctcttttgg ttattcggtt ttttcttact gcttatttta tccgcagcat tttcaggaaa
     3901 aatgacagca gatcttgctc agaaagaaac agaaaaaatc gtagcaagca aagaatcagt
     3961 agataatttc aaaaaagaat tctcaactta ttggtgggcg gagtataaaa taactgccaa
     4021 ttacgaaaaa ggcgaattgg acatttattt gccaaaagga aaagaagaaa atcaatttat
     4081 taatatcgat gacgtactta gtgcgatttc tctcagacaa tatcaattta aagacaagca
     4141 tttgaccgtt ttgcttttga ctcatgatgg cgacaaagta gcccgtttga aaaatggaaa
     4201 agtgatcacc gcttattctg aaaaagtaga caaaaaagtg gtcgccgatc gtttagcgga
     4261 atggaaaaag acaaatgaac aagtcaagaa aacaactgtc tcttctcaaa aaaacgagtc
     4321 gacaggaaca agtccttcag attctggcag tggacaaacg ataacgggaa gtgaagaaaa
     4381 tcaagcctca attattacga atgagtcatc acaaggacaa tatcaatcct atggacaatg
     4441 aaaaacagag gttgtgaa
//
LOCUS       DAZKSR010000167         4372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000167 DAZKSR010000000
VERSION     DAZKSR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.173"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1051)
                     /locus_tag="ACG8XG_002918"
     CDS             complement(83..1051)
                     /locus_tag="ACG8XG_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0774782.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="ACG8XG_002919"
     CDS             complement(1496..2902)
                     /locus_tag="ACG8XG_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0774783.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="ACG8XG_002920"
     CDS             complement(3124..3486)
                     /locus_tag="ACG8XG_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774784.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3798)
                     /locus_tag="ACG8XG_002921"
     CDS             complement(3490..3798)
                     /locus_tag="ACG8XG_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774785.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3807..4226)
                     /locus_tag="ACG8XG_002922"
     CDS             complement(3807..4226)
                     /locus_tag="ACG8XG_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774786.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
BASE COUNT         1241 a          979 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DAZKSR010000168         4071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000168 DAZKSR010000000
VERSION     DAZKSR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.174"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..1436
                     /locus_tag="ACG8XG_002923"
     CDS             87..1436
                     /locus_tag="ACG8XG_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0774787.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1564..2154)
                     /locus_tag="ACG8XG_002924"
     CDS             complement(1564..2154)
                     /locus_tag="ACG8XG_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0774788.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2301..3653
                     /gene="brnQ"
                     /locus_tag="ACG8XG_002925"
     CDS             2301..3653
                     /gene="brnQ"
                     /locus_tag="ACG8XG_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0774789.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          790 g         1361 t
ORIGIN      
        1 gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg aagtaattaa
       61 acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag cgtggactga
      121 aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg ttgtttttag
      181 gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat cttattactg
      241 gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca aacaccaata
      301 accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct ttcatcactg
      361 gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca gccatcggaa
      421 tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg attgcattag
      481 gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta gagttttggt
      541 ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct tatatgatcc
      601 tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg tctcatggcg
      661 gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta acctttgctt
      721 ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct gaaaaagtgt
      781 tgccggaagc tatcaataat attcctatca ggatcatctt attttactta ggatctcttt
      841 ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt ccatttgtag
      901 aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta gtcgtattaa
      961 gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg cttcgttctt
     1021 tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat catgtgccag
     1081 ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg aattacgtga
     1141 tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc ttgtttatct
     1201 ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc aaaaaagctc
     1261 attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt tacatggcct
     1321 ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta ctaacacctg
     1381 tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag aagtaagaaa
     1441 gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga aaattgcttc
     1501 ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt cccagcctct
     1561 tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat aggaaaaaat
     1621 ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa aaaagacacc
     1681 ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct ttgacaaaac
     1741 tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca agataccaaa
     1801 agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa aagcttcagg
     1861 gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga gcaaaaagat
     1921 attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac gatgatggtt
     1981 cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg tatttcggat
     2041 gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata caagaactct
     2101 caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg tcacgatgat
     2161 tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat aaccatcgtt
     2221 ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt acttttaaaa
     2281 aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat ctttttatag
     2341 gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc gttcatttgg
     2401 gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt accggtatcg
     2461 gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt gtatatgaat
     2521 tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt tacttagtta
     2581 tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt ggtttagcac
     2641 catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt gtattttttg
     2701 tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt ggaaagttcc
     2761 tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt ttgaatccac
     2821 taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct tttttcacag
     2881 ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt gggatcatca
     2941 ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct aaagacacga
     3001 tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg ctcgcttatt
     3061 taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc acgctggctc
     3121 agattgctga tcattattta ggtacttatg gcagtatctt attagcattt atcgttatat
     3181 tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca ttcacagatt
     3241 tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt ccttgtttag
     3301 cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg atgttcatct
     3361 atccattagc aatgacactg attttactag tgttagttgg tcctttgttt aagcagcgta
     3421 cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt gatgggttga
     3481 acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta tatgttgcgg
     3541 aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca gttatcggtt
     3601 ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat tgataaaaga
     3661 ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa atggcttctc
     3721 acatttttgc ataaatttga cttaataccg agaaactgac ttttgaacac cgtttattga
     3781 gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga aaatggcttc
     3841 tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac accgtttatt
     3901 gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag ctccatatac
     3961 tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca tttcttttat
     4021 aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaaaa a
//
LOCUS       DAZKSR010000169         4055 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000169 DAZKSR010000000
VERSION     DAZKSR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.175"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..165)
                     /locus_tag="ACG8XG_002926"
     CDS             complement(<1..165)
                     /locus_tag="ACG8XG_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774790.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /gene="panD"
                     /locus_tag="ACG8XG_002927"
     CDS             complement(367..762)
                     /gene="panD"
                     /locus_tag="ACG8XG_002927"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFQ0774791.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACG8XG_002928"
     CDS             complement(772..1620)
                     /gene="panC"
                     /locus_tag="ACG8XG_002928"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFQ0774792.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACG8XG_002929"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="ACG8XG_002929"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFQ0774793.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="ACG8XG_002930"
     CDS             complement(2474..3334)
                     /locus_tag="ACG8XG_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFQ0774794.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3572..>4055
                     /locus_tag="ACG8XG_002931"
     CDS             3572..>4055
                     /locus_tag="ACG8XG_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0774795.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     F"
BASE COUNT         1287 a          781 c          471 g         1516 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat tgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacaagcag agctaactgc ttttttagat tacgaaaaat atgatcgaaa
     3721 tggattcaat tcaggaaatt cccgtaacgg gaattattca cgtacattta aaacagaata
     3781 tggcgagttg aatttaacta ttcctagaga tcggaatggc gaattttctc aacaaacact
     3841 accagcatat aagcgaacca atgattcatt agaaacgacc attatccaat tatttcaaaa
     3901 aggcattact atggctgaaa tctccaaatt aattgaaaaa atgtatggtc accactatac
     3961 gccacaaaca atctccaaca tgagtaaatt ggtggctgaa gatgttttag cttttaaaga
     4021 aagaacgtta gaagccaatt attccgttat attta
//
LOCUS       DAZKSR010000170         4054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000170 DAZKSR010000000
VERSION     DAZKSR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.176"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..1669)
                     /locus_tag="ACG8XG_002932"
     CDS             complement(164..1669)
                     /locus_tag="ACG8XG_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0774796.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1666..2352)
                     /locus_tag="ACG8XG_002933"
     CDS             complement(1666..2352)
                     /locus_tag="ACG8XG_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0774797.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2545..3966)
                     /gene="gndA"
                     /locus_tag="ACG8XG_002934"
     CDS             complement(2545..3966)
                     /gene="gndA"
                     /locus_tag="ACG8XG_002934"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0774798.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          857 c          693 g         1389 t
ORIGIN      
        1 gttttttcgt tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt
       61 gttcgctagt ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct
      121 tattctttgt tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct
      181 ttgcgatagg aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga
      241 tacgtccttt atagttttca accagttgtt tagcgataga caaccccaga ccatttccac
      301 ccttcgttct agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt
      361 cttctttggg tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg
      421 aagaaataga gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata
      481 tgataatgat taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca
      541 aatcatcatc taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt
      601 gataggtgac ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg
      661 ctcttgataa gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc
      721 tggcgcttaa tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac
      781 taagatgccc ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga
      841 attgctcttg ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag
      901 ctaaatctgc caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat
      961 ctttacgtat agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag
     1021 ataaaaagaa acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag
     1081 tcaataatag cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat
     1141 agccgatctt ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt
     1201 ttccttctag tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt
     1261 tttcatgggt tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg
     1321 gctgccctag ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag
     1381 tatgatctac gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta
     1441 ctaatgtcag ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct
     1501 ctacattgtt tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag
     1561 cgaaaattgt aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca
     1621 aggagggccc tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac
     1681 ataacctgtt ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt
     1741 gcgaagataa cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca
     1801 cactttattt agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag
     1861 cagcaactcg tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg
     1921 gttttctttt tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt
     1981 atcgccttca atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat
     2041 agcaaatggt ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat
     2101 cacagaatct cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct
     2161 gcagacttct aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc
     2221 attatttaat gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc
     2281 ttcatgcttc aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag
     2341 aatattgctc atttattatt ttatcctttc tattctactg gctttttaaa aactctatca
     2401 ttataccgat aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa
     2461 aagagctgaa gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct
     2521 catttcctaa cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc
     2581 ttctttgtcg acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat
     2641 atttgcaggc agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt
     2701 cggtacaggt acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata
     2761 ttttttcgtg atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt
     2821 gtcataggca tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca
     2881 gatttttgcg atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa
     2941 ttgagcaaat ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt
     3001 ttcgaccaat tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt
     3061 gcttgcttgt acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt
     3121 gatcaatgga agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc
     3181 tttattccca gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc
     3241 tttacgtgtc agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt
     3301 ccattcttta aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa
     3361 gtcataagat tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt
     3421 cacataatgt ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc
     3481 ttttgcagaa atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc
     3541 aggcatgatt gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat
     3601 aaagttgatg ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt
     3661 atttcctccg tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt
     3721 tgcatctgtt gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga
     3781 ttctacaaat tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac
     3841 tacagcggtt gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc
     3901 tatgttcaag gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt
     3961 tgacatatga actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa
     4021 cacggctaca ttgcaaacta aagaacaaca tatt
//
LOCUS       DAZKSR010000171         4035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000171 DAZKSR010000000
VERSION     DAZKSR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.177"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACG8XG_002935"
     CDS             complement(243..392)
                     /locus_tag="ACG8XG_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774799.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ACG8XG_002936"
     CDS             854..1066
                     /locus_tag="ACG8XG_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774800.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="ACG8XG_002937"
     CDS             complement(1344..3143)
                     /locus_tag="ACG8XG_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0774801.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="ACG8XG_002938"
     CDS             complement(3267..3863)
                     /locus_tag="ACG8XG_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0774802.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1186 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg ttttt
//
LOCUS       DAZKSR010000172         4033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000172 DAZKSR010000000
VERSION     DAZKSR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.178"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..972
                     /locus_tag="ACG8XG_002939"
     CDS             <1..972
                     /locus_tag="ACG8XG_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0774803.1"
                     /translation="DYVRIRFPTTDVRKIIQEILHLKFEYMLHEDYAFYSYQEQYVMG
                     DIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCLKAGGVMKRLDLAI
                     NDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGMGNTLYIGSLKSEV
                     YFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLLKGRSIESTTFSII
                     NRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYTIERTLNWLGRQVA
                     PTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENLIV"
     gene            969..1082
                     /locus_tag="ACG8XG_002940"
     CDS             969..1082
                     /locus_tag="ACG8XG_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0774804.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            1852..3762
                     /gene="ltrA"
                     /locus_tag="ACG8XG_002941"
     CDS             1852..3762
                     /gene="ltrA"
                     /locus_tag="ACG8XG_002941"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0774805.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1467 a          623 c          868 g         1075 t
ORIGIN      
        1 gactatgtaa ggattcgctt tcccacaacg gatgtgcgga aaattattca agaaattctc
       61 catttgaaat tcgagtatat gcttcatgaa gattacgcct tttactccta tcaagaacaa
      121 tatgtcatgg gcgatattgt agtgatgtta tctcatgaag aagataaagg tgtccttttg
      181 gaattaaaag gtcgtggttg ccggcagttt gaaacttttt tactcgccca aaagcgtagc
      241 tggtacgact tttttgaaga ttgtctaaaa gccggtggcg tgatgaaacg cttggattta
      301 gcaatcaatg atcgagtggg gctactggat attcctgatt taacgaaaaa atgtcagaag
      361 gaagaatgta tctccttgtt tcgtaccttc aaaagttacc gttccgggga actgttgaaa
      421 gctgatgaaa aggatggtat gggcaatacc ttatatattg gcagcctcaa aagcgaggtt
      481 tatttttgtt tgtacgaaaa agattatgaa caatatatca agttagggat tccattggac
      541 cagaccgaaa cgaaaaatcg ttttgagatt cggttgaaaa atgaccgtgc ttattatgcg
      601 attcaagatt tgttaaaagg tcgtagcatt gaaagtacca cgttttccat tatcaatcgg
      661 tatttgcgat ttgcggataa agtagatgga aaacggcgaa ccaattggcc cttgaatgaa
      721 caatggggtc gttttattgg acggaatcgg aaagagattc aattaacttc tgaaccgaaa
      781 ccgtatacca ttgaacgaac attaaattgg ctaggtagac aagtcgcccc tacgtggaaa
      841 atggctaagg agttggatcg attgaatcaa acaacgtata tccaggatat ggtacagaat
      901 gcgcgactct cggaccggca taaaaagatt ttggagcaac aaagtatggc aatcgaaaac
      961 ttgattgtat gaaaagagga attttgatgg aaattgtaaa agatatgtta ttggtacttg
     1021 gtggcagtat gcttggtgtt accgtgatgt gcttgatgaa tgcaagtgca gcagcggatt
     1081 gagcaatgga aaagaaagga aagtgacaaa atgaatttcg gacaaaattt atgtgcgact
     1141 cgttccttag tgaaaagcta aggcactaat aaaagaacga aaaagtttgg ttattaggag
     1201 aactgataat ctgacaggtg gaatgatgaa gtaacgctct gaaacgcctg ccctgagcct
     1261 ccgacaagca ttgtatgagg aaactcagaa tgtttgaagc tcggtaaaga cggctgaaag
     1321 ataccgtaat agttgatgaa catatatctt ctatcgaaaa ctatccagag gtgggtagcg
     1381 tatccaacag gtcgggggtc tataaaatat ctatggttag aatgttctat aaggaactga
     1441 cgaaacagcg aatgtacggg tctaaatttg aactttgtag aaatgcaaag gtacgagaaa
     1501 tcgtagctgg tgtggcaaag taagaatata ggttatgaaa tgccatataa cgttacaggc
     1561 ggtatcaagc cagcaggctt atagctagtg acctaaggat atatatgtat agataagatt
     1621 atcggaacga ggaaaggtat tgggttctca tcaagagaat aaactgacga agaacaataa
     1681 gcagttgaac taatgctgaa aagcagaggg cgaacctaag aaattcttgt aatgagaatg
     1741 gaggaatgcc ctcaagtcgt gtatatatta gaaaagtatt atgttgtttc aataaggatt
     1801 acgagtaaga taaaaaagct cactcaattt caagtaaagg agtgaagcct tatgcctaaa
     1861 aataaaaatg aaacattgtg tgtagaagat ttaagacacg ctgaatatta cgaaatgcaa
     1921 aacacctttg atgatttata tgccaaaagt aagaatggag atattttcac tcatcttatg
     1981 gatattatcc tatcgagaga aaatatatta ctggcatatc gaaatatcaa agcaaatgca
     2041 ggcagtaaaa cagcaggaac agacggtaca ataattaaag atattggcaa attgccagca
     2101 gaaacagttg tcaaaaaagt aagatacatt gttgcaggca gtccacatgg gtatcgacct
     2161 aagccagtga ggcgaaaaga aataccaaaa ccaaatggta aaaccagacc tctaggtatt
     2221 ccttgtatgt gggacaggct tatacaacaa tgtataaaac aagtattaga accaatctgc
     2281 gaagctaaat ttagtgaaaa cagctatggt tttagaccta ataggtcagt tgaaaatgca
     2341 ataaaagcaa cctataaccg cctacaaatc agccagctac attatgtgat tgaatttgat
     2401 attaagggtt tctttgacaa cgtgaaccat tccaagctga taaaacagat atgggctatg
     2461 ggaatacggg acaagcactt gatattcatt ttaaaaagga tattaaaagc tccaatcaaa
     2521 atgactaacg gtacaataac atatccagag aaaggaactc cgcaaggggg aataatatcc
     2581 ccactactag caaacattgt tcttaatgag ttagaccatt gggtagaaag ccaatggcag
     2641 gaaaatcctg ttacaaaaaa ctatgtggtt catatcaata aaagcggtag cccatgtaaa
     2701 agcaacgctt acaaagaaat gaagaaaaca aagttaaagg aaatgtatat ggttcgttat
     2761 gcagatgatt ttagagtttt ctgccgctac aaggaaagtg ctgaaaaagc caaaatagca
     2821 atcactcaat ggattgaaca acgattaaaa ttggaagtat cacaagaaaa aacaagaatt
     2881 gtaaatgtta gaaaaagata ctccgacttt cttggtttca agattaaaat gataccgaga
     2941 agaaagaagt tagttgttaa atcacatata agtgataaac aattcaaaaa ccaaaaagac
     3001 aagctggtat cacaggcaaa gaaaattgct tctccctcaa aagggaaaac ggaattagaa
     3061 gaaacaagac tttataattc aatggtatta ggtatgcaaa attattatca gattgcaacg
     3121 aatgtaaatc tggattgtat gaaactgaat agagctgtaa tggctatctt cactaacaga
     3181 ttaggtacaa acaaaagggg cagattgaga aaaacaggca gacaacttac gacaaaggag
     3241 gcaacaagat atggtaaatc taaaatgctt cggtatgttg ctggcgaggg cgaacctatt
     3301 ttccctgttg gatatatcca acacagaaaa ccaatggcaa aggtgtattt agcgaattgc
     3361 tacactctag agggaagaaa atttatccac accaatctaa gtgttgataa attactgatg
     3421 taccagttaa tgaaattatc tttggaaaat cgcacaatag aatacgcaga caatcgaatt
     3481 tcgctatttt ctgctcaaca tggaaaatgt gctataactc tggaagaatt tcaacaagct
     3541 aatgaaatac attgtcatca tattgtacca actggagctg gtggaaatga cgattataaa
     3601 aatcttattt tggtaaaaga ggcggtacac cgtctaatac acgctaaaac agaagaaaca
     3661 atacaaaaat atatagctct attgaagcta aataaagtcc agctcataag actaaataaa
     3721 tatagagtat tagctggcaa tcaagaatta aacttgatat aggtctacta cataaatact
     3781 tttcaatata aacacgatgg aacgcggagt gcggtgaaag tcgcacgctc cgtgtgaatg
     3841 gggggaaaag tttgagatat ttataatatc aggaacttac ctatccatat atcaatggtt
     3901 tttaaccaat gctcaatctt tagtcttact agcgattgtg gtcattggtt tgtttttagg
     3961 atttaaacgg gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg
     4021 tctggtcttt aat
//
LOCUS       DAZKSR010000173         4015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000173 DAZKSR010000000
VERSION     DAZKSR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.179"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..1461)
                     /locus_tag="ACG8XG_002942"
     CDS             complement(175..1461)
                     /locus_tag="ACG8XG_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0774806.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1454..2722)
                     /locus_tag="ACG8XG_002943"
     CDS             complement(1454..2722)
                     /locus_tag="ACG8XG_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0774807.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2974..3435
                     /gene="mscL"
                     /locus_tag="ACG8XG_002944"
     CDS             2974..3435
                     /gene="mscL"
                     /locus_tag="ACG8XG_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0774808.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3494..3940)
                     /locus_tag="ACG8XG_002945"
     CDS             complement(3494..3940)
                     /locus_tag="ACG8XG_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774809.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1136 a          817 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 ttcatacatt tggatttcct gaccgatgat tcctttttcc ttgtttacag tttcttctgt
     1081 gaagtaaggt gcttgtacaa aatcgatcag agtcgtcaga ttcttttcta cctgatccgt
     1141 cgtagaaaac agatagctgg ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg
     1201 tttcccaaat aattgaaaga catcaccatc ttctttttca aacaacttat gttctaaaaa
     1261 atgggcaatt ccatctggaa ctttcacctt ttctttttca ccatagggaa taaattcgtt
     1321 atcgatcgaa ccataattcg tactgaacaa accatatgtc ttgtgatagt catttttagg
     1381 caacaaatac acggtcaaac cgtttggcag tacttcatga taaagtgttt cattgatttg
     1441 ctcatattct gttttattca tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt
     1501 cgatttgctc agctacttgt tgtatctctt tgatcgttac gctctccata cgtttcaacc
     1561 attcttcatc agataatttt gtttccggca gccagctgtc caaaaaagaa gcttcgattg
     1621 ccgcttgcgg agagtctaga gaaagcagat attggtttct cagcattgct tttgtctgag
     1681 acagttccaa ctctgttatt tcaccatttc gcaaactttc taattgctcg tgaatcagat
     1741 gaagtacttg atttcggttc ttttcatcga taccggtttg gacactcatg aatcctcgga
     1801 acgtatccac gctactagag gcataataag ctagactttc tttctctctg acattcatga
     1861 aaagtttaga gtgaggaaaa ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt
     1921 ctggttcatc gtaatacaca tttgtctgat aagctaggtt cagctttgct tgaacgattg
     1981 gctcacgtac ttgtcgttcc ttcacgatat tcacttgagg ttgggtataa aacagatctg
     2041 gataaactgt ttcccgcgac tcaaatggta attgcccaac tgcttcagct gctttgtccg
     2101 gatcaacatc gccgacaaca aaaatgtcga tttgatcatt tgccatcatt tcttgatagt
     2161 aggacgctag cgaactagca gtgatcttgt ccaaatcttc gactacgccc aaccctggga
     2221 tcttttgttc cgggctgttt tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt
     2281 gtttatcttc cttcaaactt tccaaatata gacgcagatt gttcttttcc aagtcaaacg
     2341 tcaactgatc aaactgatga tttttaatat ttggataaaa taaaacctct tttaaaaaat
     2401 ctaccgcttg agaaaaaagg tttggatcat tgacatattc accattcaca aaactgatcc
     2461 ctgcgtttag ccaatgtaaa tttccttttt ttctaactga taatccaaag cttgcgccat
     2521 aaagctctgc aagttgcgca cttaactttg tctgatccgg ataattcaaa ctatttgttt
     2581 ccaacatgct gctgagaagt gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa
     2641 aacggataaa aatccggatc gttttgtatt gttttgttgg taagacatga agattcacgc
     2701 cttctgccaa ttgatacacc atatttatat gctccttttc acagttgatt gcccgcttac
     2761 tcttttaatg ggtcaacctg ccttttttac ttattcctct gcttgaaatt tataagttca
     2821 ggcaattcgc cgttttttct aaatgatgtt acactttata gtatagcata gatgtttttt
     2881 atcaaaagaa acagacggct aaaagcgtat attgtttttt tattttttat ttttcgatat
     2941 actaaagaca aataaaagaa gggagggtca ttcatgatca aagaattcaa agagttcatc
     3001 atgcgtggag atgtcttgga tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc
     3061 atcgtaaacc aagtggtgca aggcttgatc acaccactaa taggttggtt tgtcgcaatg
     3121 atcacaggta cttctgattt agaaggagca ctttcaattc ttgactggtc gccgacaaaa
     3181 ggcgtcacat ttgcgtttgg taacgttatt agtgctatca ttacatttct gatcactggt
     3241 tttgtcttat tcttagttgt caaagcggca aaccgtgcga agaatttacg ctccaaagaa
     3301 gagcaagtgg aagtggaaga aactcctact gcagaagatt atcttagtga catacgcgat
     3361 cttcttgcca aacaagtggc agaagacaat acacatccag tagagcaaac aaatctttcc
     3421 gatacttcta aataataaag aatctaaaaa aaagaaagcc gattttcagt gtcggctttc
     3481 tttttttaga ttcttaactt gctacttgtt cctttttcat gtcgatatgt ggaatatcat
     3541 cttccaaata cacatctgag attgccttga atccaaacga ctcataaaaa gcttgtaggt
     3601 attcttgtgc gccgatcacg attggttttt ctggaaaatt tttctggatc atgttaatcg
     3661 tttcttgaac aatctttctt cctaaaccat ctttacggtt gtcctgatga acaaggacac
     3721 gtccaaaatg gatactctcc gcttcttcat atactcgcgt gtaggcaagg atcttcccat
     3781 cagtatcttg aaaaaacgta tgccaagctt gtccatcaat gtcatcaact tcttgataag
     3841 gacactgttg ttccacaaca aaaactgcga ttcgttcttt gacgatcgcg taaaactctg
     3901 ctgttgacaa ttcttcaaat ttctttacct gatattccac taagcaaacg ccccctgctt
     3961 ttttattctt agcataccga ttcgcacaca aaaaagcaaa cgaaccttac gaatc
//
LOCUS       DAZKSR010000174         3997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000174 DAZKSR010000000
VERSION     DAZKSR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.180"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACG8XG_002946"
     CDS             complement(<1..218)
                     /locus_tag="ACG8XG_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0774810.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
     gene            complement(837..1169)
                     /gene="traE"
                     /locus_tag="ACG8XG_002947"
     CDS             complement(837..1169)
                     /gene="traE"
                     /locus_tag="ACG8XG_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0774811.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2016..3056)
                     /locus_tag="ACG8XG_002948"
     CDS             complement(2016..3056)
                     /locus_tag="ACG8XG_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0774812.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT         1325 a          683 c          528 g         1461 t
ORIGIN      
        1 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
       61 ttttgatagt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
      121 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatagta
      181 tgtgtgccca atcgtatatt cggatcataa tttttcattt gtcattcctc cattacgtaa
      241 atttcatttt ctctatgttt tatatcaatt ttttcacttt attcaggact cgttcatatt
      301 ttatcgtttt tcgtttactt aaaaatacat aaaaaatatc gactcgttta tggcgtccga
      361 aacgagaaac cctagtagaa atcggtaaat gctcgattga tccaatgacc aacgtcctcg
      421 caagtatcta catgtactgc agcacacgac acaatttctt gtgcgtcctc cccattacga
      481 gaagacctac tccttattcc atgagtttgc gtcaaagcct agatccctta gtagctgctc
      541 atgattaatc taggcgctat tctaacttgt ctaacttcta aattccctct tgtttactct
      601 gtgcatatta tttacacaaa ttcatttcac cactatccaa acaagggcat ttactcgatt
      661 gaccaatttt ttacgttacc aatcgtaaaa acctaccccg tcaccccccg aaaggaattg
      721 ttgatagatc aagtctactc cttatttttc tagtaattct aagttctttt agaagtctcg
      781 acaatatttt caagcaagcg aaaaacgata aaatacaagt tatgagaatg atcaattcag
      841 ctgattaaac gccccatccc ttttgcggta caacgcaaga ctccattttt tcttcgacca
      901 atcagtacac cgttttggtc ataaacatgt ttgattgttg gatatagttc ttttaaaggg
      961 ataggatctt ttaaaattat ttgcttttgt ttaccagcgt aataaatcat ttcctctgag
     1021 actttgtctt tctggattat atatttctta cactgcacaa cggttttact ttggccagtt
     1081 gatagtttct tcgtgatttt ctctgtagcg gtacaccgtt taccgtagtg acaaatatct
     1141 tcgagactca aaattcctgt atatgacata aaataatcca cccacttact ttaaatattt
     1201 ttaataagtt tttcttgtta tttttcaaaa atagagaatt gattttcgtt catgacaaac
     1261 aaaaaagagg gaacgcatcc aaacatgcgt tccctctaat ttcagcgttc gtcatacggc
     1321 aaagagccac acggacattt ttataacatt atgcttaggc atagattcat cgtaatatca
     1381 cgtattttta accttaacac agccaaaaaa agtggtatca cttttgaata tctgttatac
     1441 taaatccgtt gcgggtaata ccgtaacact gaaaggtaaa cgcattaagg gattaaaggt
     1501 tgtcttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt ctcacagggc
     1561 ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa
     1621 ttaattcatc acaagcgtag cagacaatta aatttaaaac aactaaattt atacaaaaaa
     1681 atgtaagcga ataatattcg tattgcctga aaagaagtaa tatcaaggtt tttagctttt
     1741 ttaaaaaatg aaattacctc tcttcgcgta ttattataac taaaaaacag caacatgcaa
     1801 acgtttgttc tcttttttgt gttggaaatt aattattaac tattaacact tatgacgtaa
     1861 aaagctacgt ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct
     1921 atttctttgt aacatttgta atataattag aataataaaa aatttttaat tttaatgcta
     1981 ctccatgaag caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata
     2041 gtttttggga agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt
     2101 ttttcgataa accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt
     2161 tcggttttta atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg
     2221 acataataac tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt
     2281 ttctttgcat tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat
     2341 gtttttatta aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata
     2401 attgtctcaa aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata
     2461 ttgtgtctat tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct
     2521 gatttatcaa cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca
     2581 gcgtctgatt tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt
     2641 tccctattct ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat
     2701 tctgctagat atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc
     2761 ttttgtaata ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt
     2821 tggcagttta atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct
     2881 tcatcgattt gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca
     2941 atcttagttg aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat
     3001 tgataaaata attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcattgta
     3061 gatcctctac tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc
     3121 aaagataaat aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa
     3181 tcagaaataa aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat
     3241 attatttata attaaatact accagaagaa cgtatgaata aaatttagtt ttaaaattaa
     3301 agataattaa gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata
     3361 taaaacatac taccattact agtaatatat actaccttat ttcagtagta tatattatat
     3421 tcttcttata agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata
     3481 caataagtac taccaatgtc agatatatat aataattatg tactaccatt aaaattaact
     3541 tatttaataa gatactactg tgttaatctg aaaaaaatta ctttaaagta gttacatact
     3601 acctgttaaa ttagatacta ccattaatac ttaaattaga tactacctat atttatatta
     3661 agtactaccg ttctaggaat aagataataa aaaaaaacta ccaaattgca attaaatact
     3721 acttaaaatg taaaaaataa gaactaccca aattaggtag ttcttaataa gagaatatca
     3781 gataaatttg tacaagtcat aaaaaaatta atcttaaatt tttaaatata tatacctgtt
     3841 aattataaac tactttattt aggtagtaca taattaacag ataaaactac atgcttgtag
     3901 tttgtctagc taatgagttg catatgattt atataaaggt gtgcagcata acttagttga
     3961 agttaagaga atagaataaa tattttttgt tttgcta
//
LOCUS       DAZKSR010000175         3997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000175 DAZKSR010000000
VERSION     DAZKSR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.181"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..609
                     /locus_tag="ACG8XG_002949"
     CDS             <1..609
                     /locus_tag="ACG8XG_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0774813.1"
                     /translation="ENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMD
                     IIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFI
                     GLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTI
                     SEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            706..1059
                     /locus_tag="ACG8XG_002950"
     CDS             706..1059
                     /locus_tag="ACG8XG_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774814.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            1676..>3997
                     /gene="esp"
                     /locus_tag="ACG8XG_002951"
     CDS             1676..>3997
                     /gene="esp"
                     /locus_tag="ACG8XG_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0774815.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
BASE COUNT         1458 a          556 c          762 g         1221 t
ORIGIN      
        1 gaaaacatgg tttttaatct gagtaatgga ataattccaa gtgttagtgg agatacgatt
       61 cggtctgaaa aaaattattc tatcattgtt tttgaaaagt tagcacaaac gtctataaca
      121 ttaggaatgg atataataga agcgtatcaa agtagagatg ctttgataca agaaaacgaa
      181 ttagctgtat cgttacctga ggtcttaaaa gtgagggatt ctgggattgt ttattatact
      241 aaggaaatcg gaaaaactaa gatagagcat ctttctccct taatttcttc tgtcgttcaa
      301 ttcatcggct taaatatcta taaaagaatt actgtaaagg aaatagctaa ttatttttct
      361 gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa tgcatattac gatttataat
      421 tatatctcta agcgaaaaat ttctacggct aaaataatgt tgaaatcaaa ccatactatc
      481 agtgaagttt ctttaggttt aggattttca gattcttcac acttttcgag agtattcaaa
      541 aagtacgcag gtgtatctcc taagcaatat caattaggtt tagtggataa tttcaacaac
      601 attagctgag aggttttatt ttattatgag actatatcat ttacgtagtt tggaggagtt
      661 gaggaaattg gattttaaca aaaaattttt tgggaaattt ctgatttgag ttgtttcgta
      721 gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga caaagtagta
      781 attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca tattaatggt
      841 agcaatttta agcaaacatt atttaatctg attaagctag atgctaaaat atcaagttat
      901 ttattctttt taatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
      961 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
     1021 aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta gagtactaga
     1081 cactttaaat atggacaatt caattataaa gaaaaagaat caataattta atgaggctgt
     1141 gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata actccaatca
     1201 ttcgtttaag attctaggaa tcattcagga atgctgtgaa accgatactt tataattata
     1261 aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca
     1321 gtctcattca gtaatgcggg ataaggctcc ttattccgct atttgaatac tctgaagttt
     1381 aatctaagaa agagtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt
     1441 cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac
     1501 aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgtgc atgaaattct
     1561 ttaagtaaac tagttaacat aagtttagta agattcctat tctgtgaata atacgtatca
     1621 gatttatctt ttattcttac taagaaaata actattaagg gagttgattt gaataatggt
     1681 tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag
     1741 tatcaaaaaa ttaagtgttg gggttgcatc tgttttggtt ggtgttggtc ttgtcttggg
     1801 aacaactgaa ctagttcaag cacaagatga aatttctcca agcaccccac ttgaaacagc
     1861 gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc
     1921 tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag aactaactgg
     1981 agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc
     2041 atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga
     2101 aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat
     2161 taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga
     2221 tggattattt gctggggctg aaacaactga tacacaaact gctcctaatg gggtaattac
     2281 gtttgaaaag agcttagatg aaatttttaa agagataggc atagacaatc tcaagaccga
     2341 tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccagg
     2401 tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt
     2461 atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgaggaacc
     2521 taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga
     2581 tcataagtta acgaaaaatt ataatttctc atattcagct tccgcaaagg gcacgccttg
     2641 gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca
     2701 catggttcag gctgacaaag taacatatga tgtttctttt gaatcaggga aaaaagtggc
     2761 tgatttagca attgaacggc gcaaagatca tgaaaattat ggtgtaggtt caattactga
     2821 taatgatctt accaaactta ttgattttgc taatgcaagt ccacgtccag tcgttattcg
     2881 atatgtttta caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc
     2941 acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga
     3001 tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat
     3061 tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg
     3121 gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat
     3181 ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt
     3241 agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga
     3301 atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca
     3361 aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc
     3421 aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat
     3481 tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac
     3541 ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt
     3601 cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata
     3661 cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa
     3721 accagatgtt cctttagcta aagatgcctt ttcaatttta gatgtctata ataaggactt
     3781 cggtaatgca agtgttgatg caaatactgg tattgttaca ttcactccag ctaaaggtgt
     3841 aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc
     3901 tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga
     3961 aaacattcct gcaggctacc acaaagtgac cttcacc
//
LOCUS       DAZKSR010000176         3861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000176 DAZKSR010000000
VERSION     DAZKSR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.182"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1157)
                     /locus_tag="ACG8XG_002952"
     CDS             complement(3..1157)
                     /locus_tag="ACG8XG_002952"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0774816.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..3783
                     /locus_tag="ACG8XG_002953"
     CDS             1342..3783
                     /locus_tag="ACG8XG_002953"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0774817.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1232 a          724 c          842 g         1063 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata
     2581 gaaaaaaatg tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa
     2641 gagctctccg atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc
     2701 gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca
     2761 tctgtcattg cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca
     2821 cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg
     2881 atcaatgcag ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct
     2941 tcaatcgtga aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt
     3001 ttccatgcat acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac
     3061 atgatcaact cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa
     3121 gagattctat tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt
     3181 gaaatcccat tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa
     3241 atccaacagg caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa
     3301 gggatcatga atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt
     3361 agcgcttcga tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg
     3421 cagatcaaag gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta
     3481 ggcggagctt ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat
     3541 tcagccaagg aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct
     3601 ttgcgtgcat tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca
     3661 ttagcgatct caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt
     3721 gaagcagaaa agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa
     3781 taaaaaagca atatctttag aagaataaag caagatttac agttatttta tttactgcag
     3841 atcttgcttt tttggctctt c
//
LOCUS       DAZKSR010000177         3757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000177 DAZKSR010000000
VERSION     DAZKSR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.184"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACG8XG_002954"
     CDS             <1..607
                     /locus_tag="ACG8XG_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0774818.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            620..1528
                     /locus_tag="ACG8XG_002955"
     CDS             620..1528
                     /locus_tag="ACG8XG_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0774819.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1528..2148
                     /locus_tag="ACG8XG_002956"
     CDS             1528..2148
                     /locus_tag="ACG8XG_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774820.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            2152..2601
                     /locus_tag="ACG8XG_002957"
     CDS             2152..2601
                     /locus_tag="ACG8XG_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774821.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            2601..3095
                     /locus_tag="ACG8XG_002958"
     CDS             2601..3095
                     /locus_tag="ACG8XG_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774822.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            3109..3540
                     /locus_tag="ACG8XG_002959"
     CDS             3109..3540
                     /locus_tag="ACG8XG_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774823.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            3542..3679
                     /locus_tag="ACG8XG_002960"
     CDS             3542..3679
                     /locus_tag="ACG8XG_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0774824.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT         1321 a          558 c          860 g         1018 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
      601 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
      661 taattacgtg gggatgattc aagttcgaca aggcaataag aatacagagg ttatggaagc
      721 gactatagtt gaaaatggtc ttccctacga tttatcagaa tgtaaggtat attttgaatc
      781 agttgtaggt gggaaatatc cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg
      841 gaaaattaac tatatttttg ataaatattc catgcagtgt ttacatcgac aaacagccaa
      901 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cttattttgt
      961 cattaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caatcgaaga
     1021 tttaattgcg gatatgacgg actttattaa tgaaaataaa ggcgatttta cggactggat
     1081 gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa caggaacaca gtttccaaga
     1141 ttggagagaa ggacaagaat cagactattt ggcatggttt gaatcaatca aggatatttt
     1201 aaagacgatt gatccaggtg gagtcatgtt agccgaacta acggatgcgc gagtagatat
     1261 tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg gcagatatgg actatttata
     1321 tcagaaattg cgagcaacgc ttttcacgat tgaatacggg gagattgaag tgactgatat
     1381 tttgcaggat gatctctttt cagataatca tgaagtcgaa aaagttggaa ctgtagcatt
     1441 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
     1501 cactcttgag aaagttgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
     1561 gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
     1621 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
     1681 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
     1741 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
     1801 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
     1861 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
     1921 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
     1981 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgtc
     2041 aatcagtcat atgtagatca aaaattccaa gaagctgtaa atcaagcaga aaattacaca
     2101 aacgaaagaa ttccaaattt tacttttgaa aaaattgggg aggtatagag aatgacagat
     2161 atcgtaaaaa taaaacaaag caatgtacag gtttatccac aaactcattg gaatgctata
     2221 gaaggcaaac caacaacggt gaagggagat aaaggcgatc ctggccaagc agcaacaatt
     2281 accataggaa ccgtttctag tggttcaacc gcttctgtta cgaatgtagg aacatcatct
     2341 gcagctagat ttaattttgt tttaccaaag ggagataaag gtgatcctgg aataaatgcc
     2401 acaactacag ctgtagctac aactacagca aatgggttga tgtcgtccac agataaaact
     2461 aagttggatg gaattgcagc tggagcacag aaaaatccag gtaatgctac aactacgaca
     2521 gcaggtttaa tgtcagcaac tgacaaagct aaactcgatg gattagccaa tattacattt
     2581 gagaaagtgg ggacggttta atggctgata ttgtgcaatt aaaagaagac ggagttgcta
     2641 aatacttaaa aacgcatgta gaagctattg atggtaaaga agcattggtg cagacagatg
     2701 gagaccaagc cattgcagga cataagaatt tttcaggttc tgtaacaatc aataataaac
     2761 gtgttttgac gactgatgat aacagatatg aagtggtaaa tctggttgtc actaacggta
     2821 atacagggac agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt
     2881 taaacggaaa aagtagtggc gggaatgatt cggttatttt aactgttcca gaaggctatc
     2941 ggacaccaat tagttttgaa caactggttg ggtcaataga ccgttctact ttgaacagtg
     3001 ctcaactatc tattggcgca gacggagcca ttaaatggag aagaaattca agttatggat
     3061 cagcttattc atttgttatc acttattcaa tttaagggag gaaatctaat gaaagtagtt
     3121 tacaaatcaa tcaagcctta cggattcgag caaatcattt tgaacaatca agaaaatatc
     3181 cctgaaaact gtacagagat taaaccacca gttcctaact ggagaccaag atttgatttt
     3241 gataaaaaac agtgggttga gttagctact gaagaagaga aaaaagggaa tgctgttgat
     3301 agcgtagatg aattggctaa tattaaggca ttatacgaaa cgctgaaggc agaaaatgat
     3361 gaattgaaac agctaaattc taaagcaatg ctaaacaatg tagcaatcaa gcaagaaaat
     3421 gtcttattga aagaaaagtc agaaagttta gcgcagttga attcaaaaac gatgcttgct
     3481 tctgtacaaa ataccaagga aatcgaagaa atcaaaaaac aattacaagg tgggaagtaa
     3541 catgtattca tatgaagata tcaaactaat gtatgactgg ggctgtttca ctaacgaaca
     3601 agtaatggtt ttcgttccgt tgtgcattac tgaagaagaa gcagataaaa tcattagcaa
     3661 agaagagagc gcatcttaat tgatgtgctt tttattttga ttcaaggagt tgtcacatga
     3721 ttaatttagg ggaatgggga gcgatagcag gatcaat
//
LOCUS       DAZKSR010000178         3607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000178 DAZKSR010000000
VERSION     DAZKSR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.186"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACG8XG_002961"
     CDS             <1..190
                     /locus_tag="ACG8XG_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774825.1"
                     /translation="LVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVT
                     VYEGHFVIAFKSGIEMEI"
     gene            333..557
                     /locus_tag="ACG8XG_002962"
     CDS             333..557
                     /locus_tag="ACG8XG_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774826.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            572..1441
                     /locus_tag="ACG8XG_002963"
     CDS             572..1441
                     /locus_tag="ACG8XG_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0774827.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1422..2156
                     /locus_tag="ACG8XG_002964"
     CDS             1422..2156
                     /locus_tag="ACG8XG_002964"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0774828.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2189..3097
                     /locus_tag="ACG8XG_002965"
     CDS             2189..3097
                     /locus_tag="ACG8XG_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0774829.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3094..3574
                     /locus_tag="ACG8XG_002966"
                     /pseudo
     CDS             3094..3574
                     /locus_tag="ACG8XG_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1252 a          521 c          790 g         1044 t
ORIGIN      
        1 tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc tgattgaatt
       61 tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa ggaagataat
      121 ccagagcgtt acagtctatg aaggccactt cgtcatagcc ttcaaatctg gcatcgaaat
      181 ggaaatatga aaaccagaag taaatagccc atgactctac agtagagttg tgggttttct
      241 tttgcttgct gattataata atattttaat aactattgtg tttatcaacc aaatggttta
      301 taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg attaaagaac
      361 tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc cagactccac
      421 agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg aaggccattg
      481 ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga gaagaaataa
      541 aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc aaatagtaac
      601 agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct cacgtgcaag
      661 aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt cagactcatt
      721 tgacctgaca gcgattaatc aaattgctac agaattggat gataaaaata gaaacaacct
      781 tgttgtgcct cccggagcgt ggggtgattg gattaatggc ggcggatggt tatttataaa
      841 tgggtatcat gttgacttga ttttacgtga tataaaacgg gtagaacaaa taatcaaaga
      901 tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg gttatataag
      961 tgcaatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga acgaaagctt
     1021 atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa gcttgatgaa
     1081 cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg cgggggtaga
     1141 ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa atcaagtact
     1201 atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac tgcttgaaac
     1261 ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg aagtactcgg
     1321 tctttctctt tttgaatgct acgatatgac cgagaaactt tataaagaag tgaatgaaat
     1381 tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa aacaaatatg
     1441 atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg
     1501 gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc
     1561 ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac ggtgcttcct
     1621 ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga aaaacccatt
     1681 ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca gaggagagct
     1741 ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag aatttaataa
     1801 aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt gaacatcctg
     1861 tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa atactgcatt
     1921 tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg gaagaaaagg
     1981 ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca aatagtttta
     2041 taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt ccggggatgg
     2101 cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag atgtaataat
     2161 atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga tggatttagt
     2221 actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc
     2281 aaatattaat atacctaaag atgaatttca ggattatgat attacatatt ttgtaagtga
     2341 tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata taataatgat
     2401 gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt cctatcttat
     2461 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
     2521 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
     2581 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
     2641 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
     2701 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
     2761 gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa
     2821 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
     2881 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
     2941 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
     3001 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
     3061 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
     3121 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
     3181 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
     3241 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
     3301 actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg atttcagggg
     3361 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     3421 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     3481 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     3541 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     3601 cgtcttg
//
LOCUS       DAZKSR010000179         3553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000179 DAZKSR010000000
VERSION     DAZKSR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.187"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1838)
                     /locus_tag="ACG8XG_002967"
     CDS             complement(<1..1838)
                     /locus_tag="ACG8XG_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0774830.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="ACG8XG_002968"
     CDS             complement(1835..2575)
                     /locus_tag="ACG8XG_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0774831.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..>3553)
                     /locus_tag="ACG8XG_002969"
     CDS             complement(2565..>3553)
                     /locus_tag="ACG8XG_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0774832.1"
                     /translation="IWESIKNIFVSYFTNIYFAALNIWTGFKLTLINIWNEVVNQAKS
                     IWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWK
                     NIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVK
                     EIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPS
                     RWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNI
                     SASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT         1124 a          676 c          423 g         1330 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcatcg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atattccaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtggtaatct tccgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattga gtgcagcaaa ataaatattt gtaaaataac ttacaaatat attctttata
     3541 gattcccaaa tat
//
LOCUS       DAZKSR010000180         3428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000180 DAZKSR010000000
VERSION     DAZKSR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.188"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8XG_002970"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8XG_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0774833.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8XG_002971"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8XG_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          967 a          756 c          539 g         1166 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattacc
//
LOCUS       DAZKSR010000181         3370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000181 DAZKSR010000000
VERSION     DAZKSR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.189"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1690)
                     /locus_tag="ACG8XG_002972"
     CDS             complement(107..1690)
                     /locus_tag="ACG8XG_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774834.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1812..2396
                     /locus_tag="ACG8XG_002973"
     CDS             1812..2396
                     /locus_tag="ACG8XG_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774835.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2407..2652
                     /locus_tag="ACG8XG_002974"
     CDS             2407..2652
                     /locus_tag="ACG8XG_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774836.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2658..>3370
                     /locus_tag="ACG8XG_002975"
     CDS             2658..>3370
                     /locus_tag="ACG8XG_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774837.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQS"
BASE COUNT         1170 a          465 c          527 g         1208 t
ORIGIN      
        1 caagcgacag tatatttatt attaaatgct attgtgagaa tataactaat tagtttaatt
       61 tgaaagtaaa tatacgctat aaaagtgcat cttgtgttat aaaaaattat tttattttgt
      121 gaacataatt taacaattct ttctcggaat tgatttcttt tcttaaatag tcttcagact
      181 tattaataat attatctatt atgtctagat acatatcact tttcgattca gctaattcat
      241 acagtttttt taattcttta tcctttaatt gaaaaccctg atccatagtc actgcatttg
      301 gatatagaag cccactataa tgtttacgta aattcacttg gaatttctga ttataaaagt
      361 ctaacagatt agaaataaaa ggcggaaaat tactgttttt gtaaaattga ttatttccat
      421 ttaggttgta tagtaatttg ttaaattgct cctgatttaa tttattgtaa atccatggta
      481 atacttccat atccttatct gctaacctga atgaactagc atcggtaaga tgttcgatca
      541 taaaatctat gaacatctct ttttcaccaa aatataaata ttgattatat attgtttcta
      601 atgttgaaga tggtagcgaa gaaactctca ttttttgctg gagtatagga tcaactagac
      661 tatttaagtg attttgtaat tcttcattgt tgtttcctag aactgcaagg gaacgtaaac
      721 ggtagtaata ttctatttct gttagtactt gttgtttcgg tttaaaatca tttattgata
      781 tttgtaaatt ttgaattgtc tcgtctgaaa aatcatgttg taattctgga tgggtagcta
      841 taaaagtaag taaaaagaac ccttttgtat acgtgatctg ttgattttca ccaagtgatt
      901 tattaatttc ctctcttgat tcagctgtga acctatactt tgcaatttct ttttttagaa
      961 tatttaaata gaattcttta ttatcaaaaa ttagggcatt aagtagtctg aaagtacttt
     1021 ccctatctct atctgcttcc tcatttccat aagataattg aaaaacaaac ttaaaggctg
     1081 tcttacatag gttttctttg gctttttcgc ttagtttatt tagatagcta ttttttagtg
     1141 cagagtgaaa ttcttcaggt gtaacagaca tagcatgtat gcgacttgca tattgtataa
     1201 tttcgatcgt tacttttttt cctaatattg gattttttag gaatataatt tcataggact
     1261 tccgtataac atcctgacaa gtttcttttg ttatatctct taattcccaa gtattaccgt
     1321 tgtaagtgac tacaggatga gctgaataat ttctttgatc cttaatatat tttaaatcct
     1381 catattcata gctatcaatt agctcagttg aactataagc tttttctagt agttccattt
     1441 cccattttgg agagtttgga tcactttttc ttagtctagc tatatcttct aaaattttct
     1501 tagaagtatt atcctcgtaa atatttgcta aatcagataa tttatcaatt aaatctaaaa
     1561 taagcgcact gtaattgaca gctattgcag aacgataatt tccctgataa aaactacttc
     1621 taacttcttc taataatgga ataattctag gatttttaac tttttttatt tcatcatcta
     1681 atataaacat ataaatctcc tttttttatt tgtatgacaa gtttaccata aaaaaaaatt
     1741 aaaaagcgag acatatttat gttgttgtgt tatcataata acataaatat gtagagtgag
     1801 atgatgaaaa aatggatttg aaacaatcgt tttacatctg gttatatcct gataatccta
     1861 tgtcaataat ggctgatatt tttgtagtta gcatcttatt gtatttattt tatctaattt
     1921 tctttcacaa tttaatcggg tacatgattc ttgattggaa aggaatgttc gatatagaaa
     1981 gaaaaccatt tgctaacaat caaacctata ccgtcttcga aagtggagat tgggaagaaa
     2041 atgaagttga tgtgatccat gaaattggat tagctttatc aggaatacaa gatgctgatg
     2101 ttttggtatc aatgacaacg gatactggat acaacgctta tgaggtattt tatcttatgt
     2161 attttatatg gcgtgatggt caagaaatta tcaatggtcg tgctgaaaaa acaatcaatt
     2221 ctaatactga aatttctttt tgtatgggtg aaggggaaat gattgtatat aaagatgggt
     2281 cagcaaacat taaaacagcc tttggtgata ttgaaacaag tattaaggaa cttagggaat
     2341 atgtgcagaa cccatataat tttcaatatt tagcaggaag aataaattta gtttaggagt
     2401 aaaaatatga aaaaaattat aaatcaacca acaaatcact tgataattga aggtgttatt
     2461 ttcgtaacaa tgtattttac tgtaggaaat ggcatctcaa atacaatcat tcaaaaaaat
     2521 ccacaatacc tattatttag tctttatgct tggattgtac aaatggtttg ggtttccata
     2581 ttgatgctgt tgttttattg gatagtacgg gttattcttt tattgttaaa caaaagaaaa
     2641 aaggataggt gaataaaatg aaatcaacta aattgaaggt tgtattgtta tcgacagcag
     2701 cattaataac ccttacagca tgtggtacaa ctaatagtgc taataataac aaagacacaa
     2761 agtctagtag tacgatggtt actaaaaaca gcactacatc ggcatcaaca acaatgtctt
     2821 caatgactga aaataatgct gaaaagtatc aaaaatacga agacattaat aaaggtcaaa
     2881 agccggatga agtttataac aactttatta atacagtaaa aaatgatgaa aattatggga
     2941 atgatccatt gcaaaataaa aatctgacta tcgttcttta caaacctgat tgtccagatt
     3001 gtcaaaaagc agaatcaaaa atcatgcaag aaataaaaga aggatttagt tatgctccaa
     3061 cattaatgca tgatattacc ataaataaac cagcttacga gtattacgca gttgctgtag
     3121 atattactga tggtgtacct gagtgggtac aaaacattcc tggtctggct gatgagaaca
     3181 atcaatatca tacgccaaca gttgcagtag taacaccaaa gaaagttaac ggcacactat
     3241 actgggatgt aatgggaatg acaacaaata caactgatga aatgattgaa agtacaacta
     3301 aatattatat ggatttggga gcatataaat tatatcaaca atcagagcct gttatggacg
     3361 atcaatccaa
//
LOCUS       DAZKSR010000182         3322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000182 DAZKSR010000000
VERSION     DAZKSR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.190"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..881
                     /locus_tag="ACG8XG_002976"
     CDS             81..881
                     /locus_tag="ACG8XG_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFQ0774838.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            897..1775
                     /locus_tag="ACG8XG_002977"
     CDS             897..1775
                     /locus_tag="ACG8XG_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFQ0774839.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1788..2768
                     /locus_tag="ACG8XG_002978"
     CDS             1788..2768
                     /locus_tag="ACG8XG_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0774840.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3039..3131
                     /locus_tag="ACG8XG_002979"
     CDS             3039..3131
                     /locus_tag="ACG8XG_002979"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774841.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1154 a          543 c          691 g          934 t
ORIGIN      
        1 aactaactct tgacagataa tcaaaaagaa tttatagttg ttaccgtagt agctactaaa
       61 aataaagtga ggattaatca atggaaacga ttattcaaat catgaaaaaa tatgattttt
      121 cagagatgga gacaagagtg tataccacac tattagaaaa aggtaatctt acaggatatg
      181 aagtaagtaa aatctcaggt gtgccacgct cgaaagtata taatattttg gaaaaactat
      241 taaaaaagaa tttgattgtc gttaataaat cagaacctaa actttatcat gccatctcgg
      301 caaatgagtt tttagagaaa ttagaaaaaa gtgttaaaaa tgatctttct tttcttacca
      361 agaatctagg gatgatcaaa gaaaaagatg aagaagaaat gctgtggaaa gttgatggta
      421 tcgagtatgt tctggataaa gtagagcatt tggtgaaaaa cgcaaaagaa agtttgctca
      481 ttcaagtttg gcatgagaat ttcacggatt cactactaaa ggcattgcag caagctgaaa
      541 aaagagttga taagtttgta ctgattttat tcagttctac ccatgaatat gatcttccct
      601 tagaaaagta ttatattcat ggttttgaaa cagacaaact tgctgacttt ggagcaagat
      661 ggatcaacat cgtcgccgat gagcaagaag tcgtgtttgg taccattaac gaagaactgc
      721 aatcaaccga tgtcacttgg acaaaaaacc acgccatggt caatctagca aaagaatatg
      781 tcaaacatga tgcatataca ctaaaagtga ttgcagaatc tcctgaagaa cttaaagcaa
      841 aatatggtga gggctttgag ggaatcagaa agatttacta gtaggaggaa ttttttatga
      901 ttcggaatat tttgctgaca ctaattgtcg caatcaatct atattttatt atttactttc
      961 taagagacct tttcaaaaac aaacaaaatt tcaaagaaga accaggggat atgcgtctgt
     1021 tgcccttcac gtcatttatc atcttctttc tttcaacatt tggcgtttcc gactttgcta
     1081 tcggaaccgt cctttatcca aagttgaaat gggtcagcat gaaaaaacta cccggaacgt
     1141 tgaataccca atgtgtagta ccggtagccg tcatggcatt gtcttacatc actgccatca
     1201 acgtagggat caagacacta gctgtttgta ttatctgcca aattatcggt gcatatttag
     1261 gaccaagatt tgtcgtgaag ttaccagaga aaacgatcaa actgtttgtc ggcataggat
     1321 tggtcattgc tgcattgttg atttttgcag gacaaatgaa ctggattcca tcaaacggaa
     1381 cagccagtga attgtatggc ggaaaattaa tcttggcagg atttttactc tttgtctatg
     1441 gtgcattgaa caatatcggc attggttctt atgcgttaac gatggtaact gtctacttgc
     1501 taggcttgaa tccagttgca gcctttccta ttatgatggg ggcttgtacc ttctcagtac
     1561 cgatcggcag tgttcaattc attaaattta atgaatacag cagaaaaatc acacttttta
     1621 cttcaacctt cggtgtattg ggggtattgg tcgctgtatt cctagtcaaa tctttagata
     1681 cctatatcct gaaatggatc gtgattttgg tcttactata cagtgcatat acgatgttgg
     1741 gcagtcaatt aaaaaaagtt acagcagcta attaaggagc gattcaaatg ttatttctta
     1801 aaaaagaaga tattatcaaa agtttttcaa tgagagaagc catcgatgcg gacaaaaaag
     1861 cactgtcatt gtattcagca ggaaaggcaa gtgtgcctct tcgcaccaat atcgatgtgc
     1921 caaaaagcaa cggacaaagc ttatatatgc ctgcttatgt agaaggtggc gaaggcgcgc
     1981 taggtgtcaa aatcgtttcc gtgtatcctg aaaacatcaa gaaaaactta ccaagtgtac
     2041 cagcaacgat gatcgtattg gatccagaaa cgggtatggt atccgcttgt ttggatggta
     2101 cttatttgac acaattaaga acaggcgctg ttcaaggagc agcaacagaa cttcttgcaa
     2161 gagaagatgc taaaattggc gcgctgattg gaaccggcgg acaagcacag tcacaattag
     2221 aagcaatgtt gaccgttcgt aaattagaag aagttcggat ttttgatatt gattttgagc
     2281 gtgccagcca atttgctgaa aaaatgatgc aacaattttc tgtaacgatg cgcccaacaa
     2341 aaaccagtca agaatgcgtt gaaggagcag acatcattac aagtgtgacc acttcaaaac
     2401 gtgcgacatt ctctgctgag tgggtcaaaa aaggtgcgca catcaatgga gtcggtgctt
     2461 acacaccgga aatgtgtgaa attccacgag aaataatcaa agccgcagat gttgttattt
     2521 ttgataccat ggacggtgtt ctaaaagaag ctggggactt catttcaccg ctgcaagatg
     2581 ggtatattca aagagacagc tatcatgggg aactaggcca actgatcaat gaagaattga
     2641 tcggtcggac aagtggagaa caaatcacga tcttcaaaac agttggttcg gctgtattag
     2701 atgtggtagt tgcgacagaa atcgttaaga aagctaaaga aaataatttg ggaaaattga
     2761 ttttctaatt aaaaaggctg aaattaaaac ctttattatt gcgtttaaaa aaagaaaaca
     2821 aagaagtttg taatcaaatg gaaactgacc ccataattta aataaaataa aaaacatctc
     2881 ttgttgaatt ctagtaatga agcgacccta aaacttagac caaaaaatct aacttttggg
     2941 tggagttgtc aagaaaaacc gggcacttag ttaaaaaata agttggacgg gttatactaa
     3001 aattgacacc aaattaagag aaagcgaaga tctggattat gccaagacgt tcatttgatg
     3061 aaatatttaa aattaccgct gtaaaactgg taacggaaga agggttttct gttaaagaag
     3121 ttagtctata gctagaagct cgctctaata gcgtgtattg atgagttcaa gaagttgaaa
     3181 aatatggata acgtactttt ctcggtagag gaagtcatga aatccattcg cagaaatgaa
     3241 attacacgct tagaaaaaga gaataaacgg ttaaaagaat aggtagaact cctaaaaatt
     3301 accgaagtct tcctgaaaga aa
//
LOCUS       DAZKSR010000183         3258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000183 DAZKSR010000000
VERSION     DAZKSR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.191"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..382
                     /locus_tag="ACG8XG_002980"
                     /pseudo
     CDS             107..382
                     /locus_tag="ACG8XG_002980"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            409..708
                     /locus_tag="ACG8XG_002981"
     CDS             409..708
                     /locus_tag="ACG8XG_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFQ0774842.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            882..1433
                     /locus_tag="ACG8XG_002982"
     CDS             882..1433
                     /locus_tag="ACG8XG_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFQ0774843.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1579..1872
                     /locus_tag="ACG8XG_002983"
     CDS             1579..1872
                     /locus_tag="ACG8XG_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFQ0774844.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1865..2161
                     /locus_tag="ACG8XG_002984"
     CDS             1865..2161
                     /locus_tag="ACG8XG_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774845.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2236..3234
                     /locus_tag="ACG8XG_002985"
     CDS             2236..3234
                     /locus_tag="ACG8XG_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0774846.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1218 a          452 c          654 g          934 t
ORIGIN      
        1 atattatatc catgaagccc aatcttcttt aatagaagat tgggctattt ttatggcaaa
       61 ggagggaaaa gaaatgtttc aaaataaagg ataacgttat gtcacaaagg gagtaatgga
      121 cagtttgccg gtggaattac aagctttatg ttggaatcta atcgatcaaa acgttcaaaa
      181 gcaatttcct ttagactatc ttcaaatttt tgaattttct acggaaaaag gaaatcaaaa
      241 gttggttcat cgtcaagaag aaccggaaga aagaaaagag tatctgattt cacctaagct
      301 tcgtttgaaa tcagtgtctc aaaaaatatg ggccattgat tcgggtgagt atcaaacgat
      361 gttattacca gaagaatact agtatctaaa agaaaggaac gaaaggactt gttagaaacg
      421 gctatcgaag tactggaaaa gtgtgcacag ctggtcactg cttctgaaga gtggggctat
      481 gaatccgtta cgatggaaaa agaggaaatt gaaatgggta tgcttcctga agatgttcat
      541 ctaccacgtt tagtgatgac acatctttat atttattgcg caccagaaga tggaaaagac
      601 tatgtagttt atttcattac agatattacc agccagcgag aatttgttcg tgggttactg
      661 gtagaaggac gattggtttg gagtcagatt ggaggaacgg atgaataaga tcgattattt
      721 gagaatgatt gcgaaatgtt tagaagatat ccttaccgcg attcatcaaa ttctgaagaa
      781 aaaaggagaa agaatatgaa atattttcaa ctagtattga cacttataac ggcaatggta
      841 attgaagtgc ttgaacaaaa aatagcatag gagaaaaaga gatgattgaa aaagaattag
      901 tgaccacaca aacagaagtt tggcgagatc ctcatcatac ccatcgctat gttttcaaac
      961 gacaatgggc gatcaaagga aaagaagcgg aagataaatt agcagtagtc attactattc
     1021 gtccaacaga tacagaacca tttacgaatg atttgagtat gttattaatc gaaaagaata
     1081 tccgacaatt aggatttact ggttttattg ctgtgaatct tttttcgtac acaaaagcga
     1141 aaagtcctcg ggtatttctt cgaggaaatg atgaacaatc tttggaaata ttaacgactg
     1201 tttttacaga aaagaaaatc aataccatta tttttgcttg tggttcgatt acagcgacga
     1261 gtcaagtggc ttatgaacaa gcaaagacta tttacgatca attgagtgcg aaacaaaaga
     1321 agatgaccaa agtattgata aatgcagaag gtaagttatc ccacccacta agtattcatg
     1381 ttcgtaatga atggcaattg gcagatagtc aattattatt tcaagaaaaa taacatttta
     1441 ttttctcatg ttacttataa gtagttttac atgctttttg actttaaaaa tacatgtgat
     1501 gatacatgtt atatgaatta tcaatgtacc cagtaatatg ctattttcat tatgtaaaaa
     1561 ataacagggg gtgtgtagat ggagattgat gaaacttgtt tgagtaactc ttataaaagt
     1621 atgaatgact tttttgagga aatggaactg gattcagaat tgctctatca attttatctt
     1681 gcttttcgag ggcaacagat ttcttttcca atgaaaatgt acgatcgtga gttagttaga
     1741 aaaagaatcg aaaacatgat agaacaggaa aaaactgtag atatccgtca attgacagaa
     1801 gtttatggtt tctctgcaag atggattcaa aaagtaatta aacaaaaaga aaggagacga
     1861 gtacatggct aagctgattg tattaggtgg tttaatttac ggtggatatt ggttatatca
     1921 atatatgctt gcgcatccga atatagataa aggaaaagta attaagacag ttggggtaat
     1981 catagttgga ctcatagctc taactaagaa aaataatact aatagcccaa aaataatgag
     2041 aacttatcgc tgtagaggat gtggaaggga aatttctact agttttaatc catctgagag
     2101 tcctcaagga agagaccgct gccatacggg ctatcgacat tattgggaaa gaatcgatta
     2161 aaagattacc atttagatca attaattgtc tgatagaata agtacgtgag ggagaaattg
     2221 aaagggggaa atataatgaa aaaatggctg ttattactaa taggaatggg aattttattc
     2281 actggttgta catctgagaa tacgaggact aaagcgacta tagaaactga tacaacaatt
     2341 gagactaaaa aaagtaaaac gcaatcaact gaacaaacta caacagaaat gaagcaatct
     2401 gtggaaacaa gtgcaagttc aatcactgaa tcttcttctg aagaggttaa gaatactttg
     2461 tgggatacga ataaagcttc caaattagaa acttttgtca cacaatgggg aaagacatta
     2521 ggtcaagaat ataaaagcta caatcttcag aataatgtaa gtctgtatgg cactccttta
     2581 ccacaagccg tgattaatgg cgattggaaa atggcaatca atgaagcacc agttaccgta
     2641 caatggtcag aagatggaac tggtcatgca gatttcaatt tagtagcagt ttattctgat
     2701 gtagaaactg gagaatattt agggcagcat gtttactttt tcggttttca aaatggacaa
     2761 cctaaagttt atttaacgca acaaaatcaa ggtaatgaaa ataattatct gtactttaac
     2821 gaaactcaaa atcaacagtt aaaacaaggt ttcagtgata ttgtgaatgg acaaactcct
     2881 caaacaccag tagtagaaga atctactcaa caagcaacaa ctacaacgac acaatctaca
     2941 agtagttcag gaatttcaag ttgggaagct gctaaagaat ctgtgctaaa taataaagct
     3001 ttatggttgt atgattctcc tgataaaaat gtatcaaata ctaatttaat tagcacaaat
     3061 gaatatatga aagatctaat gcaagatgaa aatggaagct actattcaat taatattgta
     3121 acggatgatg atggaaacac gaccatggct ggtggaaaac aatttcgtgt ttatacagat
     3181 ggaagaattt ctcaacgtat tggaatgact aacaattttc aaatgattaa ataattgaaa
     3241 atattattta tcaaagaa
//
LOCUS       DAZKSR010000184         3167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000184 DAZKSR010000000
VERSION     DAZKSR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.193"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..542
                     /locus_tag="ACG8XG_002986"
                     /pseudo
     CDS             288..542
                     /locus_tag="ACG8XG_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="ACG8XG_002987"
     CDS             612..1124
                     /locus_tag="ACG8XG_002987"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774847.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="ACG8XG_002988"
     CDS             1121..2158
                     /locus_tag="ACG8XG_002988"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0774848.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2318..2494
                     /locus_tag="ACG8XG_002989"
                     /pseudo
     CDS             2318..2494
                     /locus_tag="ACG8XG_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            2549..3167
                     /locus_tag="ACG8XG_002990"
                     /pseudo
     CDS             2549..3167
                     /locus_tag="ACG8XG_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1182 a          520 c          616 g          849 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     2341 caataaattc tatcgaagtt tacgcactgc ctctacaacg attaaaggca tggaagccat
     2401 ccgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
     2461 tgaaatcaag gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat
     2521 tctttattta aaactttgca acagaaccga atcattaaaa aaacattata cagagccttg
     2581 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
     2641 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
     2701 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
     2761 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
     2821 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     2881 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
     2941 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     3001 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     3061 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     3121 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaa
//
LOCUS       DAZKSR010000185         3078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000185 DAZKSR010000000
VERSION     DAZKSR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.194"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..587)
                     /locus_tag="ACG8XG_002991"
     CDS             complement(<1..587)
                     /locus_tag="ACG8XG_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0774849.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEA"
     gene            1131..2303
                     /locus_tag="ACG8XG_002992"
     CDS             1131..2303
                     /locus_tag="ACG8XG_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFQ0774850.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(2433..>3078)
                     /locus_tag="ACG8XG_002993"
     CDS             complement(2433..>3078)
                     /locus_tag="ACG8XG_002993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFQ0774851.1"
                     /translation="SDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQM
                     HGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGK
                     KCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDI
                     LRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT         1024 a          574 c          452 g         1028 t
ORIGIN      
        1 atggcttcca tgcctttaat cgtggtagag gcagtgcgta aacttcgata gaatttattg
       61 cgtctcttta ctggacgatg gtcttgttca atcaaattat tcaggtattt aatggtacga
      121 tgttctgtcc cttgataaaa gccgtattct tttagtttct taaaggcact tgtaatagag
      181 ggggctttat ctgtgactac aaccttcggt tcatcaaact gcttcactaa ccgcttaaga
      241 aaagcatagg ctgcttgtgt gtcccgtttt ttacgtaacc aaatatccaa ggttaaacca
      301 tctgcatcga tggctcgata caaataatgc cattttcctt taattttgat gtacgtttca
      361 tccattttcc atgaataaaa ggatttttta tttttctttt tccaaatttg atagagtagt
      421 ttgccatatt cttgcaccca acgataaatc gtcgtatgag aaacgttaat gccacgatca
      481 tataagattt cttgaacttc acgatagcta aggttataac gaagatagta gcccacggct
      541 acaataatca catcctgctg aaattgcttt cctttaaaat gattcatcgt cattcctcct
      601 gctatctttt tctattattc taccttattt gatagtagat ttaaaacttt gcaacagaac
      661 cgaaaaaagt tcaaaagatc aagtgtgcgc ttcgctccca ctcccctcga cgttttagta
      721 gcctttccct cacttcgttc agtccaagcc aactaaaaag ttttcgggat acattctcct
      781 tctcttccta ataatcaatt aaaattttaa tctttatatt tctgctaatc actcaggaaa
      841 cagtacagaa aaacaaacac gtatcataga tataaatgta atggcatagt gcgggtttta
      901 ttttcagcct gtatcatagc taaacaaatc gagttgtgtg tccgtttttg ggcgttctgc
      961 tagcttgttt aaagtctctt gaatgaatgt atgttctaag tcaaaaaact ctgacagcgc
     1021 ctttatatag ataaagtccg tataattgtg taaaagtaaa aaggccatat aacagtcctt
     1081 ttacggtaca atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag
     1141 tacattttac actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg
     1201 acgtttctaa aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag
     1261 aatatattca agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct
     1321 attatgagcg cagctttacg acacgtgtag gcacgctaga attaaaagta cccagaacac
     1381 gtgatggcca tttttcaccc acagtgtttg aacgttatca acgaaacgaa aaagccctca
     1441 tggcttcaat gttggaaatg tatgtatcag gcgtttcaac tcgtaaagta tcaaaaattg
     1501 tggaagaact ttgtggtaaa tccgtctcta agtccttcgt ttctagctta acagaacagc
     1561 tagaacctat ggttaacgag tggcagaatc gtttattatc agaaaaaaat tatccttact
     1621 taatgaccga tgtactctat ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct
     1681 gtcatatagc gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc
     1741 aaagtggcga aagcgaagag acctggacaa cattttttga atacctaaaa gaacgcggtt
     1801 tacaaggtac ggaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa
     1861 aatccttcac caacgtaagt tggcaaagat gccaagttca cttcctaaga aatatcttta
     1921 ccaccattcc taaaaaaaat tcaaaatctt tcagagaagc tgttaaagga atttttaagt
     1981 tcacagatat taacttagcg cgtgaggcta aaaatcgatt gattcatgat tatatcgatc
     2041 aaccaaaata ttcaaaagct tgcgcatcat tggatgatgg attcgaagac gcctttcaat
     2101 ataccgtaca aggaaattcc cacaatcgac taaagagtac caatctaatt gaacgactga
     2161 atcaagaagt acgcagaaga gaaaagatta ttcgcatctt ccccaatcaa acatcagcca
     2221 atcgcttaat tggagccgtt cttatggacc tacatgatga atggatttat tcttcaagaa
     2281 aatacatcaa ttttgataag tagaaatggt aaaaacattg tatagcattt tacacaggag
     2341 tctggacttg actcacttcc tttattattt ttcatttttt taaactattt aatactaatg
     2401 tcttttataa tagcttttca tatattatat aatcaatctt tataagtcct tttataaatt
     2461 tcttttctac cattttcgat aaattcctgt ttaatatttt taattccata aacaatagtt
     2521 tcaataggat aatattcttc aactatatct tgatattctt ttgctttctc aatatctata
     2581 tttccataca ttcttaatat atcttctcca aaatttgttc ctatttcttc ttcactatct
     2641 tcaagtaagt atataaaatc acaatattca tctataattc cagaatctcc aaaatcaatt
     2701 attccagtta atctattatt gccatctaac aatagatgat tacaactaaa atcattatgg
     2761 cataaacact ttttaccctc aaaaactgtt gttgcattta gtctttccat aaaactttct
     2821 atataatctt tttctatatc agttaaatca ttataaatag tttcacgcaa caatatatac
     2881 tcttctaata cattttgttt attatcaata gtacattcac taatatctgt ataatctaaa
     2941 ccgtgcattt gtcttaaaaa actggcaata tctcgtttta acaaattttg ttcttcttct
     3001 gacatagtag aataaatttc tggtgttaaa aaagttcctt taatttcttt ataacctagt
     3061 atagataatt catcacta
//
LOCUS       DAZKSR010000186         3073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000186 DAZKSR010000000
VERSION     DAZKSR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.195"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1104
                     /locus_tag="ACG8XG_002994"
     CDS             289..1104
                     /locus_tag="ACG8XG_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774852.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="ACG8XG_002995"
     CDS             1230..1487
                     /locus_tag="ACG8XG_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774853.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="ACG8XG_002996"
     CDS             complement(1739..2311)
                     /locus_tag="ACG8XG_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774854.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8XG_002997"
     CDS             complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8XG_002997"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0774855.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1020 a          539 c          473 g         1041 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tag
//
LOCUS       DAZKSR010000187         2991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000187 DAZKSR010000000
VERSION     DAZKSR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.196"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8XG_002998"
     CDS             complement(<1..182)
                     /locus_tag="ACG8XG_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0774856.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFH"
     gene            876..1262
                     /locus_tag="ACG8XG_002999"
     CDS             876..1262
                     /locus_tag="ACG8XG_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0774857.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1360..1551
                     /locus_tag="ACG8XG_003000"
     CDS             1360..1551
                     /locus_tag="ACG8XG_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774858.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2065..2397
                     /locus_tag="ACG8XG_003001"
     CDS             2065..2397
                     /locus_tag="ACG8XG_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774859.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2307..2510
                     /locus_tag="ACG8XG_003002"
     CDS             2307..2510
                     /locus_tag="ACG8XG_003002"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0774860.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2541..2687
                     /locus_tag="ACG8XG_003003"
     CDS             2541..2687
                     /locus_tag="ACG8XG_003003"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774861.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            2694..2849
                     /locus_tag="ACG8XG_003004"
     CDS             2694..2849
                     /locus_tag="ACG8XG_003004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0774862.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         1099 a          487 c          457 g          948 t
ORIGIN      
        1 aaatggaaat ctttagtttt tttaatatta tcagttagta actttttata atcataaaaa
       61 aaataagtag ttctatctga gcgatactct tttataatac tgaattcgga attaaaaacc
      121 acgataggta actttaaaca aacatgaaga gactttaata agacttcttt atctgaaaac
      181 acttttgttt ctctctttcc tttctacatc attaaatagt attttatacg ttatctaaaa
      241 tactattcct ctgtcaaaat aagctaactt acttcattat aaaatatcca cttatcgctt
      301 tcatgtatat tccaccgtaa aaagtacatt ttatcaccga gaactcatca tttttcatca
      361 tgatgaatac tatttacata tatctagttc aaagacaata attattatga tccgttatta
      421 tctaagcact atacagataa taggaatttt tcgtttctaa atactccaat ttgggaatcc
      481 aataaaaatt ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg ttaattatga
      541 agggggaaga aataaaaaat acaatctata cacgaataag aatattatcc aaaaggaggt
      601 tttagaaaag atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg
      661 tttgatattt aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta
      721 tcaactaaaa aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt
      781 atagaagtta ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct
      841 tttcccttta aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa
      901 atgcccagtt gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata
      961 aaaaacaaga agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta
     1021 ctaaagatac tataaaattt aaacaaccga ttaatgtttt aaatgtgact ctccttgttg
     1081 aagatataaa attagcaaaa atccttcctt tgttgaaaga acctgaacaa atttttctta
     1141 tcgaaaaatt tatctttaat aaaactgata aagaaattgg aaaggttctg ggtattacaa
     1201 gacagggtgc gacaaattta aaacatcgac tttacaaaaa aattcaaaaa atgctggttt
     1261 aatatctgca taataactaa ttttgtatgc ttctttgctc gtcctcctcc ttccccaaaa
     1321 aataaataga ttataagttc atgggaagaa ggtaaattta tgaatacttt aaaagaagat
     1381 ttcattttag caaagaatgg aaatgaagaa gcagttgtgg ccattctcaa aagatttagc
     1441 gctttggtcc ataagcaatc ctggagaact ggaaaatatg atcaagattg ttatcaagaa
     1501 tgtatgcttg caatatatct agctatctcg aaattcgaaa ttaaagaata actcaactaa
     1561 gggagtctgt gacataagta aaaagatagt ttcaaatttc gaacaataaa tttgaagcca
     1621 tcttctttat aattataaag tatcgatttt acagcgttct ataatgattc atagaatcta
     1681 aaacgaatga ttttaattat taacgttttt taattcgttt ttagcttaaa aataactttt
     1741 gtcacagtct cactcctaaa attactttat ttacgtacga agtgggcagg aggtcgaatc
     1801 cagaactggg ccatggtact tcaacgagga cttctcctct agaattgaaa agcatgcgat
     1861 ttaccttccg taacgacggt agatttattc aactatgtgt ccaatcaaaa tttgtctgat
     1921 ttcaaaaata gtcaagagaa ttttggttgt taaaaactct tatctatcaa cttccacagt
     1981 ttagttgaca aaccctgata ctaagttggg ctatgataca cttttcttga ctatcagtta
     2041 cgttaccgga aggaatgaac tctattgaaa aaaacatatg atcgtaaatt taaactaaag
     2101 atttcacagg atatccttga gaaaaaagta actaccaaga aaattgctga agaatacaat
     2161 atctctcgtc caacaatttc ccgatgggtt tcagaatatc gtcgatacgg gaaaaatgct
     2221 tttgctggac aaggaaaaag attacctgat aaagcagatt tctacattct tgaacaggaa
     2281 aataaacgac taaaagagga agacgagtgt tctacgaaca caaagatcga tacggatctc
     2341 cagggatcac tcaaaaactt tacgatgaag gaatcgaaat caataaacgt gttgtagctg
     2401 ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca tcgacgcaaa tcttctcatg
     2461 gtagagaaaa aacaatcaaa gaaatggtaa aaaaaatgtt gaatcgataa tttgaacaga
     2521 accaaattga tgctgtttgg gtgacggata ttacttacat tccatgtaat gacggtcgcc
     2581 tctatttgtc cacctatatt gacttagcaa ctcgtattct acgttactat atggtggatt
     2641 cacacatgaa aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta
     2701 cggtaattca ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga
     2761 ccgaccacca catcacacat tcaatgagcc aaactgggac tcctgttgac aatgcagtaa
     2821 ttgaagcgtt tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta
     2881 aaatagagat gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa
     2941 tccatacaaa atttatgatg actccttgac aatttgaagc gaagaaaata a
//
LOCUS       DAZKSR010000188         2962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000188 DAZKSR010000000
VERSION     DAZKSR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.197"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8XG_003005"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8XG_003005"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0774863.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG8XG_003006"
     CDS             complement(1036..1956)
                     /locus_tag="ACG8XG_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0774864.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG8XG_003007"
     CDS             2069..2821
                     /locus_tag="ACG8XG_003007"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0774865.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          831 a          596 c          513 g         1022 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gt
//
LOCUS       DAZKSR010000189         2952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000189 DAZKSR010000000
VERSION     DAZKSR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.198"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..406
                     /locus_tag="ACG8XG_003008"
     CDS             89..406
                     /locus_tag="ACG8XG_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0774866.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACG8XG_003009"
     CDS             complement(497..862)
                     /locus_tag="ACG8XG_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774867.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="ACG8XG_003010"
     CDS             1070..1552
                     /locus_tag="ACG8XG_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774868.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="ACG8XG_003011"
     CDS             1749..2639
                     /locus_tag="ACG8XG_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0774869.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DAZKSR010000190         2932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000190 DAZKSR010000000
VERSION     DAZKSR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.199"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..983
                     /locus_tag="ACG8XG_003012"
     CDS             87..983
                     /locus_tag="ACG8XG_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0774870.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1075..1290
                     /locus_tag="ACG8XG_003013"
     CDS             1075..1290
                     /locus_tag="ACG8XG_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0774871.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1308..1580
                     /locus_tag="ACG8XG_003014"
     CDS             1308..1580
                     /locus_tag="ACG8XG_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0774872.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1582..2445
                     /locus_tag="ACG8XG_003015"
     CDS             1582..2445
                     /locus_tag="ACG8XG_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0774873.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1162 a          464 c          511 g          795 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacttttgag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggcttgaaaa atggaaactt ggcttcttct attgttcatt
      421 tgactgataa tttagacttg atccctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacttttgaa aatgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatctcatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcgagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttaca agaacaattt aaccctggac
      721 tagatatgat cggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaag caacataaag aagataactt ggttttccaa aatattatca
      841 agcgaagtaa taaagtaagt acctggtcta aaaatggaat tacagaacac aaaggctatg
      901 acaaaaaagt tttatccatg tatgagaacg tattttttga aatgcttgag cgaattattc
      961 aattagaaaa cgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacttg
     1021 tgatttgtga ttgttgatga taaaataaga ataagaagaa atagaaagaa gtgagtgatt
     1081 gtgggaaatt taggcgcaca aaaagcaaaa cgaaatgata caccaatcag tgcaaaaaaa
     1141 gatataatgg gagataagac ggttcgtgtt cgtgctgact tacaccatat tataaaaatt
     1201 gaaacagcaa aaaatggcgg aaacgtaaaa gaagttatgg atcaagcctt agaagaatat
     1261 atacggaaat atttacctga taaactttaa aaaggagtga aaatgaaatg gcagttacgt
     1321 atgaaaaaac atttgaaata gagatcatta acgaattatc ggcaagcgtt tataatcgag
     1381 tattaaacta tgttttgaac catgaactag atactaaaaa tactcgttta ctagaagtga
     1441 atcttttaaa tcaattagaa gtggcacaag aagttgattt atttcaacaa ccatttgaag
     1501 aattacaagc tattcatgag tattggcggt caatgaatca atattcaaaa caaattttga
     1561 caaaagagaa agtggcttaa catggcgaat attactgact tcaccgaaaa gcaatttgaa
     1621 gatcgtttag aaaagaatgt tgaacgacta actaaaaata gactagcggt tgaatcgcca
     1681 accgcttttt tacttggtgg gcaaccagga tcaggtaaaa ctagtttgcg atcggcaatt
     1741 tccgaagaga cacaaggaaa tgttgttatc attgataatg atacgttcaa acaacagcac
     1801 cctaattttg atgaactagt gaaactttat gaaaaagacg tagtaaaata cgttacccct
     1861 tattctaatc gcatgacaga agcgatcata agccgtttga gagataaagg gtataattta
     1921 gtgattgaag gtacaggacg aacaacagac gttcctattc aaaccgcaac aatgcttcaa
     1981 gccaaagatt atgaaacaaa aatgtatgtc atggcagtac ctaaaatcaa ctcatattta
     2041 ggaacaattg aacggtatga aactatgtat gcagatgatc caatgacagc cagggcaaca
     2101 ccaaaacaag cgcatgatat tgttgtcaaa aacttaccga ccaatttaga aacccttcat
     2161 aaaacgggct tatttagcga tataaggctt tacaatagag aaggagtgaa actctattca
     2221 agcttagaaa cgccttccat tagtccgaaa gagaccttag aaagagaatt aaatcgtaaa
     2281 gtatcaggga aagaaattca accgactttg gagagaatag agcaaaaaat ggttcaaaat
     2341 caacaccaag aaacccctga atttaaagca attcaacaaa aaatggaaag cttgcagcca
     2401 cctacaccac caatacccaa aacacctaaa cttccaggga tttaaacctt aaaaaagtaa
     2461 aagagtagtt accaaaaaac ggtaactact ctttttttat aaaaacattt cctcattttt
     2521 taaaagctga tcgagaaata aagcacacaa ctgtattctt tcttttttca gttggtacat
     2581 tttgcagtct gtaacgtgcc atttactgtt tgttacctta acatatccag tcgaaaacac
     2641 aagggtatat aaacgagcaa gctcgccctt gtgttttctt tgtgtctatg ttgcgttctc
     2701 gtcacagcaa acgacacgaa cacactggcg aaaatgaacc aacaaaacga aaaaagaaag
     2761 gagcgtacac tgtaatttat ttctagcttt taaaacatga ggaaaacagc ttcacttcct
     2821 caaacgccta acaaaaatgg ttgtcaaaaa actaaaaatc aaaaaaagaa aagaggaaaa
     2881 gaaaatggaa aaaacaaaaa aattacaatt agaagatttt acagaaaatg gg
//
LOCUS       DAZKSR010000191         2890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000191 DAZKSR010000000
VERSION     DAZKSR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.200"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..429
                     /locus_tag="ACG8XG_003016"
     CDS             <1..429
                     /locus_tag="ACG8XG_003016"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0774874.1"
                     /translation="LIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVL
                     MLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANV
                     DISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            435..1457
                     /locus_tag="ACG8XG_003017"
     CDS             435..1457
                     /locus_tag="ACG8XG_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFQ0774875.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1479..2534
                     /locus_tag="ACG8XG_003018"
     CDS             1479..2534
                     /locus_tag="ACG8XG_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0774876.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         1158 a          293 c          537 g          902 t
ORIGIN      
        1 ttaattattg gaactggtga tatggaagaa gaaataaaaa attatgcaaa gaaaagtggc
       61 atagatgatg atattaaatt tttaggaaat agaaatgata tagaaaaatt ttatcaagca
      121 tttgacgtat taatgttgcc atcattattt gaaggggttc cgttagtggg tattgaagct
      181 caatttgcag atttaccgtg tttcttttcg gagaaagttc ctacagaagt tgctttcagt
      241 gataagacca atttcataag tttaaatcaa tcggcaaaag aatgggcgag agagattgca
      301 aatgtagata tctctcatcg agattcagaa cgttcgattt taataaaggc agattataat
      361 attcaaacag catacaaaat cttagaatca aaatattatg agttatttga attaatacaa
      421 agaggttaaa gaatatgaca cagaaacatt caagtataaa aagaattata catcgcatat
      481 ccgaagaatt gaatgcaaca agacgaattg caaagataac atcttggaag gaagcatttg
      541 ccacgttaca agctaaaatt gatattcaag tcatgaatcg taatggatat aatgaaagta
      601 aaaaacagaa ggaacattta ttaaaaaaac atgaggtaat gctgaagtat tatgaaaaga
      661 ctttttcaga ttttttgaaa acatatgatt atagtcaaaa tcaaataatt aatcaatcaa
      721 aatcaccata ttctaactgc atttgggttt gttggtggca gggattggat caatcgccgg
      781 aaattgtaaa agcttgtgta aaatcaataa aacgaaatgc agggaaacat actgttataa
      841 ttttaacaga agataattat agacaatatg ttgatatacc aaaatgggtg gaagaaaaga
      901 aaaacaaagg aatcattacg agaacaaatt attctgattt attaagatta tctttgctag
      961 caaaacatgg aggtttgtgg ttagacgcca cattttattg tacaaaacca ataccagatg
     1021 agtattttga tatcgatatt tggtctatta ggaggcctga ttatgctcac gcatccgtag
     1081 catcaggata ttttgcgggt tattcattag catgtaatca agagtctaga tggatgtttg
     1141 ctataattag agactttttt ttaaattatt ggaaaaataa tgatatgatg gtggattatt
     1201 tgatgataga ttatatgatt gttttagcac agagaataaa tcatgaaata gcacagacat
     1261 ttgataaaat acctagtaac aatccattat gtgacgaatt gattaaaaat ttaaataata
     1321 tatatgatga aaaagtatgg aacttattaa agaaagatac tttactgttt aagttgacat
     1381 ggaaacaatc ttttaaaaag caaaaagatg ataaagttac tttttatggg atgttggtag
     1441 aaaacaaatt attataaata ttagaggtat aaaaagttat gaaacacaag gtaagtgtga
     1501 tagtaccaat ttacaatgtt gaaaaatatt taagaaaatg tgtggatagt ttattgagtc
     1561 agacattaaa agagatagaa atcgtattag ttgatgatgg ttcgccagat gcttcggggg
     1621 aaatagcaga tgagtatcaa aaaaaatatt ctaatgtaaa aacaatacat agagaaaatg
     1681 gggggttagg accggcaaga aatacaggaa ttgagaatgc gacaggagaa tatgtggctt
     1741 ttcttgattc agatgattgg gtagaaagtg atatgtatga aaaattatac ttagttgcgt
     1801 ccaaagaaaa tgctgatata gtagttagtg gacattgtga ttttgcgaat gacaaagcta
     1861 tggtgaaaaa gcaacatcct ttgagtggga atgtatatga tacgcctaat agtattaaaa
     1921 atgtacgaaa aaatttattt ggacatagtc caaaagatca agaagtagag gccttcccga
     1981 tgtcagtatg tatgtctttg tataataaaa atctgctaga taaatataga ctaagatttg
     2041 aaaaaatttt atctgaagat acaattttta acttgaatgc atatgatgtc gcaaataaaa
     2101 ttgcttttac agaatataca gattattgtt atagaaaaga agaacaagag tctattacaa
     2161 aaagtttatc tccaaataag cagacggaat ttaaagaatt tttaataact ctaaaagcta
     2221 tagcatcgaa agaagattta gaatgtcaaa tcagagcaaa acgtatggca attgattatt
     2281 gtaggcttta tgtgggctta gttgatgatg caaatttggg aattaaagag aaaagaaagt
     2341 atataaaaaa atttattgaa gatgagggat taattcaatg ttggaaggga tatcctacaa
     2401 atacgctacc atttcaacaa aaattttttc ataaaatgct tgaatgtaag tgttatacaa
     2461 tattgttgtt gttaagtaga attagaaaga taattaaaaa tatgaccaga aaaaaattgt
     2521 ttataatatt ataaactaaa agtatatcaa ataatagaaa ttaacaagtt acaaaaaaat
     2581 attataaaga ataatatttt ctaaaaagta aactatagta atgtggatta tcaccttcca
     2641 acagtatata aaatgagcgt aaaaacggct atttttgcct taacaacgat ttttttgtat
     2701 acaaatacga tatgctcatt aattattgga atttgtagtg ctgtttaaat taatgaaggt
     2761 gtaattatat gagtgatcat tttgaaaaag tttgttgaga agatgtgctt ctagtcatgt
     2821 gatgggagaa aataatttaa ctgttagagt gaatagttaa attattctgg ttatatgaaa
     2881 aaagtagttt
//
LOCUS       DAZKSR010000192         2875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000192 DAZKSR010000000
VERSION     DAZKSR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.201"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..433)
                     /locus_tag="ACG8XG_003019"
     CDS             complement(<1..433)
                     /locus_tag="ACG8XG_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774877.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
     gene            complement(992..1309)
                     /locus_tag="ACG8XG_003020"
     CDS             complement(992..1309)
                     /locus_tag="ACG8XG_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0774878.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1310..1564)
                     /locus_tag="ACG8XG_003021"
     CDS             complement(1310..1564)
                     /locus_tag="ACG8XG_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0774879.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1824..2048)
                     /locus_tag="ACG8XG_003022"
                     /pseudo
     CDS             complement(1824..2048)
                     /locus_tag="ACG8XG_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2602..2688)
                     /locus_tag="ACG8XG_003023"
                     /pseudo
     CDS             complement(2602..2688)
                     /locus_tag="ACG8XG_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          951 a          510 c          408 g         1006 t
ORIGIN      
        1 tcatatttcc ttgattacct gaggcaatga aagacttatc tacttgaatt agggtgaaat
       61 tctcattcaa atatggtagt aaaattaata tatgtgaaaa tcgaatcttg agatagctat
      121 tccccttgag atattcactg atacttctac tcaaatcatt taaattttta taattatttg
      181 tacggaacgt atatatatca acatcagttt tctttacatg agaacttatt ctaaaatgaa
      241 aaagcctact ctcagaaaga attgaaaaat agatactttc agtagatact gaagattgaa
      301 caaaattaaa cttgtatcct tcaggcatag cacttttaac agtctgctgc attaactcgg
      361 ataatctttt gaaagtagta atatattttt taccagcaaa ccatacatat cttttttcat
      421 taagtatgtt cactatctct cctccattaa ttatatttag catcaaatag ttattgtacc
      481 tatattttat cccattcctt ttaaaacaag aatacgtaca aagtaaaatg ttgtaaatag
      541 tctagcaggg atgtatattt tcatgtacca acataatatt taagaagttt cccatagata
      601 tattggactt cctcttttaa aacacaaaaa taaatactac cttatttagg tagtatttat
      661 taaaagttac gtcaattttg attaaaaata tactacttga acctctcatg agtaaagtca
      721 cgaaattttt gggaatagag cgtacttgat atcttatttt ttcactaata gcagtgtctc
      781 ttattcacca agcgatcccc gtagttccta cggttcttaa tttttctcta gactaatgat
      841 tgtatcttta gaccttcggc cataacatgg agcaaaataa aaaggtacta agtcttatat
      901 atcaatcttc gagtaaccga cggttaacaa ccatatcgag cggcattaat cacccacctg
      961 tagaggatga gcgacttttg cctaattatt gttaaaagcc tagtttcatt tgtttagatt
     1021 ggaaaatacg tcctacaagt atctcttttt tttcatggtc gatccgataa aataaggcat
     1081 agcttttgcc gatcacgatc cgatatgttg ggatatccat tccatagatt tcggaaactt
     1141 cctcatgcat agttggaaaa tttttggtta gttctaaagc cttctggatc gcacctacaa
     1201 atctttttat gctggctgga gaaagagcaa gctcattttc ccattcttct attatcgttt
     1261 caaggctggt ctggaaactt ttagaataaa ctagcgtgta ctctttcatc tattcactcc
     1321 agccgtattt tccgaaagct tttaccattt caatttctga aactgttaaa cctttctcaa
     1381 agtcttggaa actttcttca atcaatttgc gatcgtgttc ttttgctatt tgagtagtat
     1441 ctaatatgaa taagttgcct tcttgtttgg cttggatagt gtctccgctt tttaatccaa
     1501 gagagcttgg aaggatcact cccacgctat tgccgatctt tctaaccttt agttcttttc
     1561 ccatgttatc acacttcttt tagtttttga taaataaatt ataacatgtt ataacgacaa
     1621 ttgccaacgg tgtttcttaa gggattccac tccatattat tattggacaa aataagccac
     1681 ttagaacgct gaaaacatat acaaacgata tcaaaatacg agtgaactac tcaccataca
     1741 cttccgttaa catccattta agtaggtatt tttattatat atagatttaa catatgtcgg
     1801 tcggttgatt gatatagcta aagttataac tcaaccgaag ccgattcatc tacacgaata
     1861 aagttgcgag tgttctcggc taattcataa aaattgttat gtgttccata tagcaatact
     1921 ggaataactt tattttcttg aatctcataa gcaactctat acacggtgac cgcatactta
     1981 aatccattag accaattacc tgataaacct ccagtttttt gctcttactt aaatggatca
     2041 atagcaattt catcacaaac ctatagagga tgagtgactt ctgcctaatt acgttaaaaa
     2101 atattgaaag taactctatg tagcaaaaaa tatctagaat ttcatttcct caaaactgat
     2161 cgatattgta tctaaaaata tatactacct aaaataggta gtatatatta aaatatgtgc
     2221 ttttgttaga aaaatcaata cataaattat catatctgta tatgtcattt aggtatttca
     2281 gttatgttat aatagttttg ctgtctgcta ccccggccta atctggcaag gggaggagga
     2341 gatataatga aatacctttt tgattttgtt tttgcaatca agccatttgc agaggtaact
     2401 gcacactaca tctgcaagtg gcttgattgc aaaattgcca aaaaagacag taagcctacc
     2461 agttaattct ataatataga aacgtaaaaa aaaccccatc agcctacacg ctgatggggt
     2521 ttgatgatat aatgaaatac ctctttgtta ctcataatta taccataatt agtcacgaat
     2581 gcaaccaata gaaaaaacta cttagaacgg tagatcatcg ctaataacaa tgcattgtcc
     2641 accaaatgga ttttgtgaag cattaaatac attgttatta attgtcgctg tttttaacta
     2701 ggcctctccc cttctattat aaaagaagat atatattatt tcttaattac ctactacctt
     2761 aaaccaaaat ttaagtacta cctaatttaa gtagtactta aataatctaa aaaatagtta
     2821 tcccaattgt tatgctttta taatacgatt atttgtaaca aaatgaatat tatat
//
LOCUS       DAZKSR010000193         2513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000193 DAZKSR010000000
VERSION     DAZKSR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.203"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(491..1783)
                     /locus_tag="ACG8XG_003024"
     CDS             complement(491..1783)
                     /locus_tag="ACG8XG_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0774880.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2057..2317)
                     /locus_tag="ACG8XG_003025"
     CDS             complement(2057..2317)
                     /locus_tag="ACG8XG_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774881.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          847 a          463 c          380 g          823 t
ORIGIN      
        1 atattataaa ttcacatgat tatcgaattt tattaagtta gtttttcttg taaatctctg
       61 ttcccaatga aactcgagac acttatttta aagaggaaac aatgtggtat ttaaatagcc
      121 ctcttgtatg ggctactctg tgatacttat tagccattaa actattttac ccacatagca
      181 tatattttga aatcgttcat tccgtaaatt caactaaata tgcctaaaaa aataaaaaat
      241 ctaagataga ataagattta ttcagtctta gatggattta tagcaacttt ttcgtaacaa
      301 ctttaaaaaa tacgaaaaga aagagctgtt ttactctgac tacgttgcta aattgtcgtt
      361 taatcattca tttaaaccaa atcttttttt atagaattcg ttttaatcct cctgaatcca
      421 aaaactatga aaaccgtaat taaatagtta agagttttta accaccataa ttctctcggc
      481 tattttgaaa tcagataaat tttgatcgga cacatagttg aataaaccta ccgttgttac
      541 ggaaggtaaa tcgcatactt ttcattctag aggagaaggt ctcgttgatc attaactgat
      601 taagcaccat agcccagttt tgaattcgac cacctgacca cttcgcgtgt aattctttcg
      661 tgcgtaaata aagtagtttt aggagtgcat tctcattaga gaacgctcct ttttttgtga
      721 cttttctgaa gctggagtgg acactttcaa ccgcattggt agtgtacata atttttcgaa
      781 tggcactacc ataatcaaat agttgttcaa catgtgcaaa gttccgtttc cagacatcta
      841 cagcaccaga ataatgagac caccgatttt gaaaactgtc aaaagcagca tgtgcagcgt
      901 ttagagaaga agcaccgtag aactttttca tatctcggca gacttcctta tagtccttac
      961 ttggaatata gcgcaatgca tttcgaacaa gatgaacaat acagcgctga acaattaccg
     1021 atggaaagat cgcttttgcg ccttcttcga ggccagaaac accgtccatc gaaatgaaaa
     1081 agacatcttc gacaccacgt gctttcagtt catcaaatac ttgcatccag cgatttttag
     1141 attctgtttg atttaaccat aatcctaaaa tctccttatt tcctttgaga tcatagccaa
     1201 gaatggtgta tacagcatat tctttggctt cataattttc tcgtaaagta acatacatac
     1261 aatcaacgaa tagaaaggca taacactttg ctagggggcg ggcttgccat tcttccaatt
     1321 caggaaggac agcgtcagtg atatctgaaa tcatttcatg ggaaatatca aagccataaa
     1381 tagcttcgac ggttgcggca atatctcgtt gactcattcc tcgtgcatac atggaaagaa
     1441 ccttcccttc gatgtcggag acatctcgtt ttctcttagg aattaactct ggttcaaagg
     1501 aagcttcccg gtctctagga acatcaatag ctacttcacc aaaactggtt ttaagcgttt
     1561 tagttccata gccatttcga cggttatcgt gttccttagg ctctttagaa tgggcatcat
     1621 aacctaaatg attattcaat tctccttgaa gcattttttc aaaaaggggc ccaaacacat
     1681 ctttcaaagc atcttgcatg tcatcgacag attcaggttg ataggcattc agaatggatt
     1741 cagctaactt ttcggcatca ggatttcttt tctttctagc catcgtgtaa tcacctcttg
     1801 atcttattgt agaaaaagaa aaaccgcgaa gcaaccacct gcctaggatt aatggttact
     1861 tacacggttt atattacact ctcatcatta aaaaagtatc tttcgatatt actaaacaat
     1921 gaaattatct atttgaaatt taaatctgat aatatattga gtgttccaat gagggctttg
     1981 atcctattat ccatatcttt ttcagtttta atattgatac acattataag aaaaagaaac
     2041 ttttggttaa tttatttcat cccagtgcaa aatataaaaa agtaaaatag accataggca
     2101 atagattaac aattaatata atacaagcaa atattctatt cattgtattt ctgctttcaa
     2161 aaagttctaa tattgaaagt ataattccac aaatgcctat aataagtatg ggaataagat
     2221 tagaaaataa caccccgagc caatctgtga aaattactat atttactaat atccctatta
     2281 acatacctat catatttatt aaatcaattt ttttcattag atatttctcc tttattaaat
     2341 ctgaagtact tatattaaaa taattctaaa ctatttacct caaaaagtgc aataaattat
     2401 ttctgaacaa taaaatgata gtcaaaacaa aaaaatattg ttaatcgttc agagccatcc
     2461 gagagtgtaa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa ggc
//
LOCUS       DAZKSR010000194         2508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000194 DAZKSR010000000
VERSION     DAZKSR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.204"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..230)
                     /locus_tag="ACG8XG_003026"
     CDS             complement(<1..230)
                     /locus_tag="ACG8XG_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0774882.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTS"
     gene            complement(342..1685)
                     /locus_tag="ACG8XG_003027"
     CDS             complement(342..1685)
                     /locus_tag="ACG8XG_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0774883.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(1819..>2508)
                     /locus_tag="ACG8XG_003028"
     CDS             complement(1819..>2508)
                     /locus_tag="ACG8XG_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0774884.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          742 a          447 c          324 g          995 t
ORIGIN      
        1 gaagttgttg aagactcagc atttgaagtc gtactacttt cacttgataa agttgtcaaa
       61 agttcactac tctctgctgt tacatcggaa ttactcattt cagtcgtctc ggttctttct
      121 tttgcaattt tgtatgttga attttcgctt gccaacacat ggaaaggtgt tgctataaag
      181 agttgggaca ttaatataac tgctgatgca agttttaaag attttttcat ttgatttacc
      241 tctctttttt ctctttaata gtgttttcta ttaaaaatta tgataaatat tttcaatgaa
      301 ttgagtaata cagtatattt cttgatttca tttagtaacg attatgtttc aatgtttaat
      361 cctgttactg cctttaaaat aaaattatag ggttggctaa aactaggatg aaataaataa
      421 tctaacattt tcaatttgtc taatgtaatt tgttcttcta tagcaagaga aaacattgat
      481 aatagtccag ataaatcttc ttgactacaa agttgtacac ctactattct ctttgtattt
      541 ttttcaaagc taattcttaa ttttacacga ctattttttc tcaaaaaaac aggtctaatg
      601 aaatcttcta tttctatata atctatagac tcacttaata atcgtgctgt ttttaaattc
      661 aaacctgctg cgtaaaaatt gtaattaaat atagaaaagc catttgtaaa ttgggaacca
      721 gaaggtgtaa aatcttgatt gtcaatatgt tgagttgcaa tataagctgt tcttagagca
      781 ttagaaatag aaaaatcaac actacttcta ttaatggacg taaaaaaagt tgtagcacaa
      841 tccccaatag catatatatc ttttatattt gtttgctggt attcattagt tagatatgct
      901 ccactaacat ctctcaagag ttcctttttg ccaattggac tattagatag aaatcctata
      961 gcttgtacga ccatatccat agagtattgg gtaccacttg tagtgactat tttttcaatc
     1021 ttttttcttc ctaaatattt tttaggactt tctgatagac aaagatgtat tccatttttt
     1081 gatagtattt cttcaacttt atctgaaaaa ctacggtcgt aataagtcga caacactcta
     1141 tctgcaacat cgaaaagata tacttctttc cctctttttc ttatggcttc agcaatttca
     1201 actccaatat atcctgctcc aataattcct acttttttaa tagtattatc atctaaagct
     1261 ttaaaaatct ctttagcatt tggaaaattc tttatcgaaa aaatattagc taaattattc
     1321 cccggtacat ctaacataat ttgctttgaa cctgtagcta gtataagttt gtcatattct
     1381 tgggaaaatc ttgtgccatc tttttttaca caaataatct ttttattttg aatatcaatt
     1441 ttgtcaactg ctgtctccaa aaacaacttt tttatcttag aatttagttc tcttctattt
     1501 tgataaaaaa gatcttctaa attgctaact tctcttttta tgaacaacga tgtcccacat
     1561 tctaagtaac taatttctga ttttttatct atcagaataa cttcataatt tgaatcacat
     1621 aagtttagaa atcgagctgc agctagcccc gaatggtttg atccaataat tactattttt
     1681 ttcacttaat cacattcttt tcccttctat gaaaagtatc ttcattttta actaacaacc
     1741 tatctcccaa aataaaaaac aaaaagagga agaaaaagat tcttcctctt ttcagagaag
     1801 atttaagaaa ttgattttct attcattctc tttttttcga tttttatacc ctaataaccc
     1861 taaagttcct aacaggatta aaccaccact aatagtaaag atattactat caatctctcc
     1921 cgtcttagga taagcttttg aggactgctt ttgaggactg cttttgcctc tatttttgtt
     1981 ttgcttgcta ccttgattgt cagtcttatt atctttacta acggcttttt cactgtcttt
     2041 ttcgtttgaa gaaacaacta cattagtagg ttcactagta ttaccagatt catcagttgc
     2101 agttactatc acttttgttc ctgcctctaa tttaggaact ttaactgtaa aattaccatt
     2161 tttatcagct gtcccatggt attgactccc gtttggtaat gtaactgtca cctctgcatt
     2221 tggttcagtt ttaccagtaa caatttggtc accctctgta ataggatcaa cttgaggttt
     2281 attcggtgct tttttatctt ctacagaaat agtaacctct acttcttctt tagatccatc
     2341 tggatatgtt actactacaa tccctttttt gtttccagct gtactaacat ctactttctc
     2401 tttccaagta taagtagttc cttttggtag atcttctttg ttcttaattc cctcagatgc
     2461 gtctggctct ttatttaagt ctgtagtcac tttttgtcct tcaggatt
//
LOCUS       DAZKSR010000195         2432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000195 DAZKSR010000000
VERSION     DAZKSR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.205"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..2282
                     /locus_tag="ACG8XG_003029"
     CDS             129..2282
                     /locus_tag="ACG8XG_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0774885.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          426 c          538 g          636 t
ORIGIN      
        1 gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt
       61 ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag
      121 gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa
      181 taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc
      241 gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg
      301 gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga
      361 ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag
      421 ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag
      481 atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc
      541 tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca
      601 caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg
      661 tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca
      721 tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg
      781 ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga
      841 ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca
      901 gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag
      961 gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc
     1021 tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc
     1081 aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat
     1141 tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca
     1201 aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc
     1261 tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag
     1321 gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag
     1381 gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc
     1441 aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag
     1501 tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag
     1561 gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc
     1621 agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc
     1681 cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata
     1741 atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca
     1801 catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag
     1861 tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag
     1921 tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg
     1981 cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca
     2041 aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg
     2101 cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag
     2161 acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca
     2221 taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat
     2281 aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     2341 agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg
     2401 tttactcggc aaaaaaggtt gtgacaaaaa tt
//
LOCUS       DAZKSR010000196         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000196 DAZKSR010000000
VERSION     DAZKSR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.206"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8XG_003030"
     CDS             207..2069
                     /locus_tag="ACG8XG_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0774886.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8XG_003031"
     CDS             2069..>2398
                     /locus_tag="ACG8XG_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0774887.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKSR010000197         2370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000197 DAZKSR010000000
VERSION     DAZKSR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.207"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            581..1540
                     /locus_tag="ACG8XG_003032"
     CDS             581..1540
                     /locus_tag="ACG8XG_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0774888.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="ACG8XG_003033"
     CDS             complement(1527..2132)
                     /locus_tag="ACG8XG_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774889.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          795 a          385 c          360 g          830 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc ttgggaagag
     2281 agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac caagctatcc
     2341 ccgtagttcc tacggttctt gtttatttga
//
LOCUS       DAZKSR010000198         2244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000198 DAZKSR010000000
VERSION     DAZKSR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.208"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            418..612
                     /locus_tag="ACG8XG_003034"
                     /pseudo
     CDS             418..612
                     /locus_tag="ACG8XG_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(800..1576)
                     /locus_tag="ACG8XG_003035"
     CDS             complement(800..1576)
                     /locus_tag="ACG8XG_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0774890.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            1699..2106
                     /locus_tag="ACG8XG_003036"
     CDS             1699..2106
                     /locus_tag="ACG8XG_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFQ0774891.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT          760 a          363 c          350 g          771 t
ORIGIN      
        1 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacggaa aaatagttcc
       61 tcatattttg ccgtttgttg agctaatacc gaaggtcagc tgattgaaca ccgtttaaga
      121 aagaggtcgt gagcctgtca tttaactctg agtcacaagt aacaatttca aaaaacagct
      181 tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga atcatctttt
      241 tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt gcccttaatg
      301 aggacagtcg gaacaggttt gtcattttta tatttaaatg gcacaaattt ttctaattat
      361 ttgtcattta gcgctgaaaa tttttgctat ttatctaaaa aagtgataac tacttaagat
      421 gaagcagaat atttacgcca tacggcatac aaccgcgaaa aatataaaaa aaagaaaacg
      481 attgaacgcg tttttgcagt cgcaaaggaa aagcatggaa tgcgctacat caagtatcga
      541 gacttggaaa acaatcaggt gtatacgctg attgtttttg ctgcaatgaa tctgaaaaag
      601 ttgtccactt ggcagttgga acatatgtct tcatatcttt tattatacaa aaatagcaaa
      661 agcaccagtc tacactctta gtaaagagga aactgatcct tttgtctaca ttcttaaagt
      721 acttaaacta agtactttat tttactcttt aggattgata cagtagcatc tatcccttta
      781 ttaataatct tttatttttt taaataaaga tgttgtctcc atcttcaggc actaaaactt
      841 tttcggaaat tcctttttct ttattgaaag actttaattc ttctcttgac agtccccaat
      901 ggtttacagc ttccatatga acggcaataa ttgtggaatc tggtattgct ttatgcaatt
      961 catataaatc gtctttcccc ataataagag attctccaac taaaaaatga ttatctccag
     1021 catttgctac tatgatttct ggattatact gatcaatagt ttgtttaaca gcttcatacc
     1081 aaactgtatc tcctgtaaag tatagtgtct tctcagtcga atgtttgaaa acaataccgc
     1141 aaacattacc tacataattc aagatttctc ctctgccatg tttgcctttt gttttaacca
     1201 attgtataga ttcaaaaact gtgtcttctc gtagtacctc cacattttta aaaccatata
     1261 gtcgaatttc attcgcatca ttttcatctt gcacaaataa ttttatttct ttaggtagga
     1321 cctctttcgc tatatcgtcc caatgatcta gatgtaaatg tgttacaata acagcatcaa
     1381 taccgttgat aatatcttcg actggcattg gcaaatcaac tagaggattc atttgatctt
     1441 ttcttggtga tggaaaagga cctttcgaaa taaatggtgg aagtgttcct ttttccgcta
     1501 acattggatc aattaaaaat tttttacctc catagtctaa gatagctgtt gcattacgaa
     1561 tgtgatgtat attcataaac gttgtctcct ttttgttgat atgaaatatt tttagtataa
     1621 ttcaattcca tacaataaat aaaccgaaca aaaaaagtct tttactattt ttacttaatt
     1681 tatgaaaggt tgtttaccat gttagaccaa acagatataa atatcattga ggaactatca
     1741 aaaaatagtc gaatcacaat gaaagaactt ggagaaaaag tacatttgac tggacccgct
     1801 gtctcggcga gagtgacaaa actagaggaa agtggagtta ttgaaaatta tacagtaaaa
     1861 ttaaatatgg aaaagctagg gttttcgatt cacgcttttt taattataat tacgcaaaat
     1921 ttaaatcata aaccctattt atcatttatt aaaaatcatg aaaaattctt gattagaaat
     1981 tataagatta gtggaggggg ctgttatctt ttggaatgta gatttacttc aaaaaaatca
     2041 atgaacagct ttttggaaca gcttaatgaa tatgcgaatt ataaattatc tattgttata
     2101 gattaataat aaaactagca gatggtaaaa atactaatat gcgagacaca cattcaacca
     2161 taactatata acttatcaat ttaaaactaa actgatggaa acaagtaagg caaaaacatt
     2221 tatattttgc cctacttatt tttt
//
LOCUS       DAZKSR010000199         2125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000199 DAZKSR010000000
VERSION     DAZKSR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.209"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8XG_003037"
     CDS             <1..159
                     /locus_tag="ACG8XG_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0774892.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8XG_003038"
     CDS             242..859
                     /locus_tag="ACG8XG_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774893.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACG8XG_003039"
     CDS             1023..1355
                     /locus_tag="ACG8XG_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774894.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACG8XG_003040"
     CDS             1679..1840
                     /locus_tag="ACG8XG_003040"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0774895.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACG8XG_003041"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACG8XG_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAZKSR010000200         1999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000200 DAZKSR010000000
VERSION     DAZKSR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.210"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..374
                     /locus_tag="ACG8XG_003042"
     CDS             <1..374
                     /locus_tag="ACG8XG_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0774896.1"
                     /translation="VSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRF
                     PEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDW
                     QKVMAAEVIATYLDGDLVYKK"
     gene            422..1822
                     /locus_tag="ACG8XG_003043"
     CDS             422..1822
                     /locus_tag="ACG8XG_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0774897.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          551 a          339 c          432 g          677 t
ORIGIN      
        1 atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt gtaacagtca
       61 ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt ataagagcag
      121 tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc ttcacgacaa
      181 ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta tcggagtacg
      241 gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac ctctgtactg
      301 atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat ggtgacttag
      361 tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga gaggttaaaa
      421 aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga tttttatctt
      481 aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg agacagttgt
      541 tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc accaaatcga
      601 taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg aagggattat
      661 ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg ggattatcct
      721 aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg gagacaaggg
      781 gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa cctttggcat
      841 gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt ctcttggcta
      901 tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat atgttgggtc
      961 gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt acggaaatcc
     1021 cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa ttgttttcac
     1081 catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt atgagagtga
     1141 tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt caacacaaga
     1201 taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg gtatcatttc
     1261 caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc tttttgctgg
     1321 gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag gctgttcaga
     1381 tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa tcttggaagg
     1441 tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg gtcttccaaa
     1501 attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt ttgttccatc
     1561 ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg atctaatgaa
     1621 tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa tgaatattat
     1681 ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta atttctctaa
     1741 atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg ctgttttgtt
     1801 atacattgct acactctttt agtcttaatc atccattaat aggaggcaat agttatgatt
     1861 aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct caaagaattt
     1921 ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg tgctccgcat
     1981 tttttcatag atgctttta
//
LOCUS       DAZKSR010000201         1815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000201 DAZKSR010000000
VERSION     DAZKSR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.211"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG8XG_003044"
     CDS             <1..580
                     /locus_tag="ACG8XG_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774898.1"
                     /translation="ELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFF
                     DQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVL
                     LLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIV
                     LFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            567..>1815
                     /locus_tag="ACG8XG_003045"
     CDS             567..>1815
                     /locus_tag="ACG8XG_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0774899.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLF"
BASE COUNT          604 a          232 c          285 g          694 t
ORIGIN      
        1 ggaattgtta ctttatgatg taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag
       61 taaattgatg aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa
      121 atgtatcttt tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat
      181 tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc
      241 tagagcaagg acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga
      301 agtcttgcta cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat
      361 ttctttttca ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt
      421 aattttaaat gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt
      481 tcttttctgt gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg
      541 ggcagaatta agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat
      601 tagttaagtc cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca
      661 taactattcc tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt
      721 tcacttcatt tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta
      781 ataatgcaag ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc
      841 tagctacact ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa
      901 atgctttttt tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta
      961 gttttgtaat tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt
     1021 tgattttatc ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga
     1081 cattatattt tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt
     1141 tgataggttt agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa
     1201 aacagtggag atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata
     1261 tagttttagc acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg
     1321 gaatatattc ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta
     1381 ataacgcctg ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga
     1441 agaaagttag taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag
     1501 tttcgattga tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag
     1561 tggttccgat aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg
     1621 aattttatga gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta
     1681 atatactatt aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa
     1741 ccgtatttac gtatttttta ctttttattt ttcattataa tatcgctaaa aaaattacga
     1801 ataaacaatt atttg
//
LOCUS       DAZKSR010000202         1780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000202 DAZKSR010000000
VERSION     DAZKSR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.212"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..759)
                     /locus_tag="ACG8XG_003046"
     CDS             complement(106..759)
                     /locus_tag="ACG8XG_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0774900.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1047..>1780
                     /locus_tag="ACG8XG_003047"
     CDS             1047..>1780
                     /locus_tag="ACG8XG_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0774901.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFH"
BASE COUNT          570 a          326 c          256 g          628 t
ORIGIN      
        1 atgttttagg aaagataaaa aaaattaatg aatgaactaa tataattatt attccaagaa
       61 cagaaaatat ttgtatgaac atttttggaa atatctttaa ttttttcatt ttctctccaa
      121 tttatatcct acatttctta tagtagttat acagtctaat tgtaattttt ttctaagttc
      181 ttttatatat acatctatta ctcgatcata aggagtttct tcgctatctt tccatacata
      241 atcaataatt tgcattcttg ttaacacttg tccattatta tccaataaac attttaatac
      301 ttctagttct tttgcattta catctatttc ttcaccattt attttagctg tatagctatt
      361 aaaattaacc gatagatcct tatattcaaa cttctctaaa tgtccataat tcttcttaat
      421 taaagaatct attctagctt ttaagaccgg caatgaaaat ggcttttcta catatccgtc
      481 tactaaatta gtaaatgcat caattttata ttcttcatca ctaaatgcag tcaagattag
      541 aattggtaaa ttgcttttct ttctaatttc tttcaacact tctaaaccat ttataaaagg
      601 tatctgaatg tctaagataa ccaaatttat atcgctatta aattttgaca aggcttctct
      661 tccatcttta gcttgaataa cagtatatcc aaattctgaa agatattcac ttattccctc
      721 tcttatcata ttatcatctt caacagttaa tattttcata tatacctcca cttaagccta
      781 tacactagat attatacagc atattatggc tctataatat ctagtgttgg tgacaccttc
      841 tgttgccaat actttttctt ttttctaata aatgcaaaca gacaccttga aatcctttag
      901 aataaagggt tctataatat tttgtgttgt tggaaaatcg aaggcgattt tctactaaca
      961 ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa
     1021 catcacccta ccaaaggagt cgctctatgg ataatcatac tagaaaatta ctcggattaa
     1081 cagataaaca tttatttttt gatgaagaat ggctgattga aaaagaatat aaaggcgttc
     1141 aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat
     1201 cgattcgtat tatccgcaac ggttcctata cgacacgtac tcagatattg aaagtcaaag
     1261 aaaagttgac tattttagaa ttaaaacgta cccgttttct atgctatgat tgtgggcaga
     1321 ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc
     1381 aggcgattct catagaactg tacgaaaatc aatcacgtaa actaatcgct aagaagtact
     1441 tcgtctcgga tgggacggtg acacggatct tgcgtgaagc aacgaagcat taccagccac
     1501 gtatgaactt ccttccgacg gtgttgtgta tggatgagtt caaatcaatg aagtctgttt
     1561 ctggttccat gagttttatt tgtgttgatg ggacaaccca tcagctattt acgatccttg
     1621 aagatcgcag actttacaaa ctaactcaat acttcctgcg ctttcctaga aaagcccgtt
     1681 taaaggtcaa gtacctagtg atggacatga atgccagcta ttgtcagttg cttaaaacag
     1741 tctttccaaa tgcggagatc gtaactgacc gctttcatat
//
LOCUS       DAZKSR010000203         1710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000203 DAZKSR010000000
VERSION     DAZKSR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.213"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..476
                     /locus_tag="ACG8XG_003048"
     CDS             54..476
                     /locus_tag="ACG8XG_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774902.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            486..689
                     /locus_tag="ACG8XG_003049"
     CDS             486..689
                     /locus_tag="ACG8XG_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774903.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            900..1484
                     /locus_tag="ACG8XG_003050"
     CDS             900..1484
                     /locus_tag="ACG8XG_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774904.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          602 a          242 c          311 g          555 t
ORIGIN      
        1 ttaagattta ttaagaaaac gttttcttat ttttgatttc gaggtgatag acaatgaaaa
       61 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      121 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      181 taactccttt agataggaac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      241 ctgaaaatta tttaatagtt tctccattac ttgctcgagc agcttctaaa acgatacgag
      301 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      361 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      421 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      481 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      541 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      601 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      661 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      721 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      781 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      841 tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt
      901 tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca
      961 gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta
     1021 atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt
     1081 ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga
     1141 atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt
     1201 ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga
     1261 tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc
     1321 gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta
     1381 cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt
     1441 tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg
     1501 ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg
     1561 tctactgata acttattctc aaatgttgta aggattttat tcatttgtta atattaaaga
     1621 acggaactcc aaaaaagtcc gtactctaat ttgcagtctc tcactctttt agagtgagag
     1681 tttttattag attttcaaat taattagtaa
//
LOCUS       DAZKSR010000204         1655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000204 DAZKSR010000000
VERSION     DAZKSR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.214"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1145
                     /locus_tag="ACG8XG_003051"
                     /pseudo
     CDS             1..1145
                     /locus_tag="ACG8XG_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1426..1655
                     /locus_tag="ACG8XG_003052"
                     /pseudo
     CDS             1426..1655
                     /locus_tag="ACG8XG_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          593 a          293 c          356 g          413 t
ORIGIN      
        1 aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca
       61 gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca
      121 aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat
      181 tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta
      241 ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc
      301 gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat
      361 attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa
      421 gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca
      481 gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc
      541 gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat
      601 gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaaaatgc
      661 cgatcgcttt agcgactgtt gcacgtagag aagcacgtta tgtagtaaaa catctttttg
      721 aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc ctctgcatta agtgtctttg
      781 actatcattt tgcagcaagc ggattgaata gctttacagc agcaaaagca ggagtagatg
      841 tacgagtgtc ttattatgaa gatacgttac gaccaaaata tgtaccagaa gaacaaggaa
      901 atccaaaagt gtccatccaa ttattctcgg atccaatgac ccatcaattg ctaggaggag
      961 cagtattatc tacctatgat gttacaggtc aaggaaatgt attggcacta gcgatccaaa
     1021 acaaactgac tttagaagaa ctagcagaag ctgacttctt cttccagcca ggctttgacc
     1081 gacaatggag catgttgaat ctagccgctc aacaagcgtt aggagaagaa ccatttgtag
     1141 aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag ctgccttgca tcaagtaata
     1201 agaacagcca agtaaaaaca gcttcttatg tttttaactt ggctggactt attaccgcag
     1261 atgcaagctt ttgaacaccg tttattttca aaaaagggac tgtgacacga tttgtgtacc
     1321 gacccccaaa agttagacca aaaaatctaa cttttggagg tcggtatttt ttatggttaa
     1381 atatactttt gaacttaaat taaagatcgt tcatgattat ttggatggca aaggcgattg
     1441 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaaattcg ggagaagcac
     1501 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     1561 aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa aagtaacggc atacacgcgt
     1621 aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DAZKSR010000205         1609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000205 DAZKSR010000000
VERSION     DAZKSR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.215"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8XG_003053"
     CDS             complement(52..1518)
                     /locus_tag="ACG8XG_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0774905.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          537 a          334 c          292 g          446 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaact
//
LOCUS       DAZKSR010000206         1594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000206 DAZKSR010000000
VERSION     DAZKSR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.216"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8XG_003054"
     CDS             288..695
                     /locus_tag="ACG8XG_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774906.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8XG_003055"
     CDS             709..1110
                     /locus_tag="ACG8XG_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774907.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG8XG_003056"
     CDS             1112..1483
                     /locus_tag="ACG8XG_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774908.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          520 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DAZKSR010000207         1573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000207 DAZKSR010000000
VERSION     DAZKSR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.217"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAZKSR010000208         1561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000208 DAZKSR010000000
VERSION     DAZKSR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.218"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8XG_003057"
     CDS             132..344
                     /locus_tag="ACG8XG_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774909.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG8XG_003058"
     CDS             506..1024
                     /locus_tag="ACG8XG_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0774910.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG8XG_003059"
     CDS             1031..1543
                     /locus_tag="ACG8XG_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774911.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          615 a          181 c          242 g          523 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 t
//
LOCUS       DAZKSR010000209         1466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000209 DAZKSR010000000
VERSION     DAZKSR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.219"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..144)
                     /locus_tag="ACG8XG_003060"
     CDS             complement(<1..144)
                     /locus_tag="ACG8XG_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774912.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQA"
     gene            419..961
                     /locus_tag="ACG8XG_003061"
     CDS             419..961
                     /locus_tag="ACG8XG_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774913.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1117..>1466
                     /locus_tag="ACG8XG_003062"
     CDS             1117..>1466
                     /locus_tag="ACG8XG_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0774914.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          569 a          183 c          280 g          434 t
ORIGIN      
        1 tgcttgcttc atccatttat cgaaagaaga aggcgtaagc tcatactctc gaataatttc
       61 tgcgcgaggc ttaccagcga gatagagatc gacgatttgt tgtttgaatt cttttgaata
      121 ggttcttcgt tgacgtcttg acatgaaaaa tcctccagtg tgttttttat tattctacac
      181 accttatttt ttctgtctag tctagtgtag ccgattcaaa ttgatgaagc agatcccaaa
      241 gggctgcttc atcaattttt tcgtaagtgt ccgtacactt actgtgcttg ctcatatatt
      301 aatcgatcat atactaatcg tgtaagaaca actttaagca taatacttat attgttatac
      361 catagtttgg aaaaataaaa aattatattt catcaacgtt tcataaaagg tgtgtagaat
      421 gttattgaaa gtagctataa aatgctattg tgtaaaaaat aaaggaggaa taatttatga
      481 aaaaatagta ccaagaggag catgcctatt acttgcatta tgtttgagct taagcgcagt
      541 tgggtgtcaa aaaagtaatg aaaacaataa aataaaagaa ggacaatcca taagttctga
      601 agaggaaggc atgtcaatta atgcaaatgg caaaaatgaa acttttaagc catcagatta
      661 tacattggag gcaaagaaag aatatgttta tgaatattta gggcttaaat ttaaactctc
      721 agacaaattt agaaattata tagctgataa aaagatagcg atgttagatg atcaaagtcc
      781 tatagataaa gaactaaagt atgctatttt gacatttgag aaaatgacag aagaacaaaa
      841 gaatgcagtt atagaaaaga tgggcgatgg atataaaaat tggcaaaatg agcttgaaag
      901 aattgggact attggtatat ttgaaaaaaa tacatcagaa gaaaaaaatc taaaattata
      961 aaatgtgata ctcatactaa aataggagtt tcaagtgatg gaaaatacga ttgctattta
     1021 agcacaaata gcggagctga aagtaatctt ctggatgaat taaaaagaac tgagattcaa
     1081 attatagata aaaaagaacg tcctaaaaat ggttttgtgc tatcagaaaa aactgattta
     1141 gaaaatacag aagcatttaa caaagaatcg gttaaagatt tacgtaaact accaacaaag
     1201 gacataaatg gcaaggattt tacaagtaaa gattttgaga agtatgatct tactatggta
     1261 aatgtatttg caacttggtg tacagcttgt gtaaaggaaa ttcctgattt ggttgaggtt
     1321 cagaatgaaa tgaaaagcaa gggtgtaaat atcgttggag ttgttacaga tcctgttgat
     1381 gataacggtg aaaacaagga agcaattgaa aagtcaaagt taatacatga aaagacaaag
     1441 gcttcatatc ctttcttaat gccaga
//
LOCUS       DAZKSR010000210         1445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000210 DAZKSR010000000
VERSION     DAZKSR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.220"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(415..1029)
                     /locus_tag="ACG8XG_003063"
     CDS             complement(415..1029)
                     /locus_tag="ACG8XG_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0774915.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          472 a          277 c          241 g          455 t
ORIGIN      
        1 aaaaggtatc ctgttggaca attttgttat atcaggtaaa cttcattgca ataatgataa
       61 caaatgtact ataattactt tgtaaagagt tttggggaag actctttcat caacgtgtat
      121 taagagacaa gaaagaatcc atatggattc tttcttgtct ctttttccac tactttttat
      181 ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca acttataaaa
      241 ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa
      301 aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct
      361 ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt
      421 tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa
      481 gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac
      541 tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa
      601 accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa
      661 tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc
      721 attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg
      781 tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg
      841 tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc
      901 atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag
      961 catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca
     1021 taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat
     1081 ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc
     1141 aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac
     1201 gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat
     1261 aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt
     1321 gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag
     1381 ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca
     1441 aaaaa
//
LOCUS       DAZKSR010000211         1418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000211 DAZKSR010000000
VERSION     DAZKSR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.221"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8XG_003064"
     CDS             complement(150..1337)
                     /locus_tag="ACG8XG_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0774916.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DAZKSR010000212         1342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000212 DAZKSR010000000
VERSION     DAZKSR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.222"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ACG8XG_003065"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ACG8XG_003065"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="ACG8XG_003066"
     tRNA            203..276
                     /locus_tag="ACG8XG_003066"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="ACG8XG_003067"
     tRNA            316..405
                     /locus_tag="ACG8XG_003067"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="ACG8XG_003068"
     tRNA            420..491
                     /locus_tag="ACG8XG_003068"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="ACG8XG_003069"
     tRNA            507..579
                     /locus_tag="ACG8XG_003069"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="ACG8XG_003070"
     tRNA            589..661
                     /locus_tag="ACG8XG_003070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="ACG8XG_003071"
     tRNA            697..772
                     /locus_tag="ACG8XG_003071"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="ACG8XG_003072"
     tRNA            774..854
                     /locus_tag="ACG8XG_003072"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="ACG8XG_003073"
     tRNA            861..934
                     /locus_tag="ACG8XG_003073"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="ACG8XG_003074"
     tRNA            937..1009
                     /locus_tag="ACG8XG_003074"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="ACG8XG_003075"
     tRNA            1021..1092
                     /locus_tag="ACG8XG_003075"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="ACG8XG_003076"
     tRNA            1138..1208
                     /locus_tag="ACG8XG_003076"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="ACG8XG_003077"
     tRNA            1229..1313
                     /locus_tag="ACG8XG_003077"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
BASE COUNT          318 a          303 c          352 g          369 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tt
//
LOCUS       DAZKSR010000213         1338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000213 DAZKSR010000000
VERSION     DAZKSR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.223"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..894
                     /gene="erm(B)"
                     /locus_tag="ACG8XG_003078"
     CDS             157..894
                     /gene="erm(B)"
                     /locus_tag="ACG8XG_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0774917.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1064..1324
                     /locus_tag="ACG8XG_003079"
                     /pseudo
     CDS             1064..1324
                     /locus_tag="ACG8XG_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          511 a          221 c          219 g          387 t
ORIGIN      
        1 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
       61 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      121 tttgtttata gatgtaatca cttcaggaga gattacatga acaaaaatat aaaatattct
      181 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      241 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      301 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      361 gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      421 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      481 attattaaaa aagtggtttt tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa
      541 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      601 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      661 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      721 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      781 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cattacttat
      841 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
      901 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
      961 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1021 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1081 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1141 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1201 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1261 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1321 ataaacataa aagccctt
//
LOCUS       DAZKSR010000214         1337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000214 DAZKSR010000000
VERSION     DAZKSR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.224"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACG8XG_003080"
     rRNA            <1..1337
                     /locus_tag="ACG8XG_003080"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAZKSR010000215         1321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000215 DAZKSR010000000
VERSION     DAZKSR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.225"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..895
                     /locus_tag="ACG8XG_003081"
     CDS             <1..895
                     /locus_tag="ACG8XG_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0774918.1"
                     /translation="AVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTR
                     EKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMI
                     EEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLK
                     ELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVP
                     FEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGT
                     VTFTKLAKAEQYRRSLELSKD"
     gene            967..>1321
                     /locus_tag="ACG8XG_003082"
     CDS             967..>1321
                     /locus_tag="ACG8XG_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0774919.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLD"
BASE COUNT          576 a          155 c          213 g          377 t
ORIGIN      
        1 tgctgtttct gtccctctta ttttaaataa acgagagaaa aataatttaa agaaaaaaat
       61 gctcattcca tttgattcag aagtgaaagg attactcaat aaactaacta tttctaatat
      121 agactcgact agggaaaaaa tcataaaaat atttgataaa attaataatt tattcaagaa
      181 tgacaatgct gaattaagga agggtctttt agagtttaaa cttcaagaac tagaacttca
      241 ttactcttat ttagtgaaaa ttaccgaaga aaaagaacaa caaaaagcaa taaaagaaca
      301 gatgatagaa gaggaaaaag ttcgtagaga aattgatcgt gaaaagaaac gtattgataa
      361 agaacaacgt caattcaatt ctgaaatttc caaattaatc gcatatatgc aaaaagctaa
      421 tgcagatgtg gaaaaagaat tatatgcaaa taaaattcaa gaactggaag aaaaattaaa
      481 agaattagaa gttatacgtg aaaatgtatt acaaagagaa ttaaatacta gagccggtta
      541 tgtgtatgta attagtaata ttgggtcttt tggtgaagac atatataaaa ttggtatgac
      601 tagaagatta gaacctatgg atagaataaa agaattgagt agtgcatcgg ttccatttga
      661 atttgacgtt catgcgatga ttttttcaga ggacgctccg tctttggaaa ctaaattgca
      721 taatcatttt agaaaacaag aagttaataa aattaatcaa agaaaagaat ttttcaaagt
      781 ttcactagac gaaatagaga aagttgtttt agaaaattat aatggaacag taacatttac
      841 aaaattagct aaagctgaac aatatagacg atctttagaa ttgtcaaaag attaaatata
      901 taaaaagcct tcgggctttt tcttttaaca attaatagga acgtatattc gaaaggaata
      961 gaaataatgt ggttagaaaa aataaatgac aaaaaattta agtacaccga acgttatacg
     1021 gatcccttaa ctgaaaaaaa gagaaaagtg tccgttactt tatctagcaa ttcacgccaa
     1081 gcttggaatc aagctaactt acttttgaat gaaaaaatcg cagaaaaaat aaaacgaaat
     1141 gaagaattac cactaacttt tggagaatta aaaactaaat gggatgaaaa atacaagcca
     1201 actgtaaaag aaagttccta tcgaacaact caggtttatc tatcattaat ttcaaaatat
     1261 ataaaagatg atgtacttgt aaaaaacgtt aatagtaatc ttatacaaga tatgctagat
     1321 t
//
LOCUS       DAZKSR010000216         1319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000216 DAZKSR010000000
VERSION     DAZKSR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.226"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(370..443)
                     /locus_tag="ACG8XG_003083"
     tRNA            complement(370..443)
                     /locus_tag="ACG8XG_003083"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Ile,seq:cat)
     gene            complement(998..1312)
                     /locus_tag="ACG8XG_003084"
     CDS             complement(998..1312)
                     /locus_tag="ACG8XG_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774920.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          487 a          208 c          195 g          429 t
ORIGIN      
        1 taattaacta ccactccaat tataagtctt tttcccatta ttttttacag ttatatggta
       61 tgctttttaa tggcttcaaa tataaaagag tttaaagcgt aacacactta tggggaagtg
      121 gtttttgggg aacgctttaa actcttcttt attattatct cacaatataa cccaaatgtc
      181 tttccattta aaaatcaaag taaaactttt caaatataca gaagtataac tatgtgaaac
      241 atcctttcat taatccataa aaggatacat aaaaaagcca ctcatttgag tggcaatgaa
      301 gaaaagcttt agcttgtata atactcttca aaaaattcta acacagaacg attcaattgg
      361 ctacattaat gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct
      421 ctgaccaact gagctaaggg tagtggttgt tgccacataa agccataaac aatcaaccag
      481 tagaatgcgt ggcaacaaac ctgttatcgc atatcttgga gtgtgactat ttatgagtga
      541 tagtgaagat atgcgtctcc atatatgtat ggttgagaca gataaacatg tatgaaaaac
      601 gttgttaaat caacgttttt ttattttggt caaggagaga aaagtagaga aatacaaaat
      661 tttttgcatt gattttgcat tcaaaaaaac attcaaacat ttagaaatcc ccaatacgta
      721 ttcaggaaaa tctattttaa ggtttacaaa aagaactttt gttcgtacac tctttttgag
      781 gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaagcatg ttccttcaaa
      841 taagtttttt aattataaac tctagagaaa ttaattcatc ctaacataaa ataattgttc
      901 actcaaaagt ttttctagaa caattaaaac agcaggcttc acattcgcta tacatttgta
      961 agaagtaata attcctgtac taaaaggcat aattgcttta attatttttt gacttctctt
     1021 caaacacaag aagtagttgt gtaataattg cagaaataag gattccaata atagatataa
     1081 aatttgacat ggaaacgtct ataacattaa aagaataaag tgttgaagaa agtaaactga
     1141 ttgaaataac ccctccgtaa aaaatagtct gtgaatatac ccactttttt tcagaagacg
     1201 tacttcgttt ggttctaaaa cctatccctg catttctact cttatcaaaa aaccaaatat
     1261 ttagaagtaa taatattgct attattaaga taaaattact gtaaaaatcc aaaagctat
//
LOCUS       DAZKSR010000217         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000217 DAZKSR010000000
VERSION     DAZKSR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.227"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8XG_003085"
     CDS             <1..1244
                     /locus_tag="ACG8XG_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0774921.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKSR010000218         1276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000218 DAZKSR010000000
VERSION     DAZKSR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.228"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACG8XG_003086"
     CDS             35..532
                     /locus_tag="ACG8XG_003086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0774922.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="ACG8XG_003087"
     CDS             complement(569..1252)
                     /locus_tag="ACG8XG_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0774923.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAZKSR010000219         1270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000219 DAZKSR010000000
VERSION     DAZKSR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.229"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8XG_003088"
     CDS             complement(31..1203)
                     /locus_tag="ACG8XG_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0774924.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          344 a          265 c          245 g          416 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc
//
LOCUS       DAZKSR010000220         1226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000220 DAZKSR010000000
VERSION     DAZKSR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.230"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1168)
                     /locus_tag="ACG8XG_003089"
     CDS             complement(join(42..940,940..1168))
                     /locus_tag="ACG8XG_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0774925.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          344 a          211 c          199 g          472 t
ORIGIN      
        1 ggatttgagt caatatcttg gacacatttt agtagagacg gttaagccat tttttctttt
       61 gctaattctt caaattcctg tggggttaga tagtgaatcg agctatggat tcgatttcgg
      121 ttataaaatc cttcaatgta gctaaatagc gctcgatttg cttcttcaaa atctgagtag
      181 ctagtcgtgt agacttcttc ttttttcaat gaggcatgga aagattcgat tccagcatta
      241 tcataaggtg ttccttttct actataagaa tgccttattt tgttggtttt aagccattgt
      301 tccacttctc ttgctgtgta ttggctgccc aagtcagtgt gtagaatcat tccttctgga
      361 atgtgataat gtatttttgc tttattcaat gtttgaatga cacaatccac agtcattcgt
      421 tttgaaaatg tataactaat aatttttttc gtgtgtaaat ccatgattga agataagtaa
      481 caccaaccat ttttcttagt aggaatgtac gtaatgtcag ctgcccattt ctcacagatc
      541 gtttcagtag aaaagtcctg atttaagaga ttctcttttg aaatgatcgg tttattagac
      601 ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt cattagcttt
      661 tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg gatctttgtg
      721 gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt caacttatta
      781 tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac acttctaggg
      841 atttctaata accgacataa caaagaaaca gtgtaatcct tacaccattt ttcaataaat
      901 tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt aagatttcaa
      961 gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt ccagtcagtc
     1021 ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct tcagataagc
     1081 catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca acaatcattt
     1141 gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat aagtttaatt
     1201 tattcttgtc tctacgaaaa gctgtc
//
LOCUS       DAZKSR010000221         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000221 DAZKSR010000000
VERSION     DAZKSR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.231"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8XG_003090"
     CDS             414..947
                     /locus_tag="ACG8XG_003090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0774926.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKSR010000222         1185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000222 DAZKSR010000000
VERSION     DAZKSR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.232"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1169
                     /locus_tag="ACG8XG_003091"
     CDS             <1..1169
                     /locus_tag="ACG8XG_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0774927.1"
                     /translation="YKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQ
                     RFKCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYR
                     VMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPY
                     LERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNH
                     RVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIA
                     DRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFL
                     PQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          363 a          239 c          237 g          346 t
ORIGIN      
        1 tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt
       61 gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg
      121 agctgaagaa acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct
      181 ctgttgctga aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct
      241 taaaagagaa tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg
      301 tctatcgtgt catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa
      361 aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta
      421 tcatgatgga cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt
      481 atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg
      541 ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt
      601 tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag
      661 tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa
      721 aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac
      781 gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt
      841 tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg
      901 taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc
      961 cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg
     1021 cgcttcacta cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat
     1081 taaaacgcaa tgcgtacggc tttcgcaact tttacaattt caaactacga attatgattc
     1141 gtcatggcaa aacattttta accaaataaa cagaacagaa agagg
//
LOCUS       DAZKSR010000223         1120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000223 DAZKSR010000000
VERSION     DAZKSR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.234"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8XG_003092"
     CDS             <1..969
                     /locus_tag="ACG8XG_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0774928.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          384 a          198 c          222 g          316 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct
//
LOCUS       DAZKSR010000224         1105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000224 DAZKSR010000000
VERSION     DAZKSR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.236"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8XG_003093"
     CDS             <1..1087
                     /locus_tag="ACG8XG_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774929.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAZKSR010000225         1092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000225 DAZKSR010000000
VERSION     DAZKSR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.237"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..591
                     /locus_tag="ACG8XG_003094"
     CDS             <1..591
                     /locus_tag="ACG8XG_003094"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFQ0774930.1"
                     /translation="KSEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFET
                     TAKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDH
                     NKVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDH
                     ESAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          426 a          147 c          208 g          311 t
ORIGIN      
        1 aagagcgagc aagacaggaa cgagagaaca aacatactca tgaaaagact agaggcttcg
       61 acctcgaact ttaataataa gttagatgtt tttgaagaaa agactaagca tgcaagatta
      121 aactttgaaa caactgcaaa gcactatatt aaacgacttg atgaagataa tttgaaatta
      181 gattttcaac aagctattca agatgaatta agcgatacta aagacgaaat acgtgaagtg
      241 acgaaacaag cgagagaaga aacaaaagag tataaagaaa tcttagaaag taaaataaaa
      301 gaccataaca aagtggttga taaatcgaat acagcgctaa aagtgatgac aaaaggcgtt
      361 acaaatatat tttttgtgct aattattttt gtacttgtaa tgcttgtgac aggtccgata
      421 ggtcatttct ttggtattga acatttatat agctttatta atggttttat tgatgaccac
      481 gaaagtgcat ggcgatattt gatgttgata ctttatgcag taccctatgt attcttcgct
      541 atcattctgt ggctcatagc attgtttttt agagtattta atgaccttta ggtagattta
      601 tatataaaaa gggctaaata cgcaaaatac ccccttaaaa tgcaaaataa atgcaaataa
      661 gggggtaact ccttatgaaa aaacaactaa tagtattata acacgttttt atgccgagaa
      721 aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg gttggacttg
      781 aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct gtaggttccg
      841 accaataagg aaattggaat aaagcaacga aaggagttga gtaaaatgag tgagaaaacg
      901 aagaaaatca ttcgagtatc attagctttt tcacaagtag tagtgcaatc attgatagtt
      961 tattatactt accagcaata taaatggttc tgttgcaaag ttttccaaaa aatctatttt
     1021 agtgtaaaat tgagaaaaaa gacagagagg acagagtaat gaatcatttt aaaggcaaac
     1081 aattcaaaaa ag
//
LOCUS       DAZKSR010000226         1091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000226 DAZKSR010000000
VERSION     DAZKSR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.238"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..971
                     /locus_tag="ACG8XG_003095"
     CDS             <1..971
                     /locus_tag="ACG8XG_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0774931.1"
                     /translation="LLPINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATG
                     RNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHD
                     KATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYL
                     SEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYH
                     VSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          375 a          201 c          216 g          299 t
ORIGIN      
        1 ttttattgcc aattaacgga tttgcctata ctatcaacaa tgaatttaat ttgggcgcaa
       61 atgaaggtag ctcacaagta gcaaataatc agtatatttt actgcatgaa acggctaatg
      121 aaacagcaac aggacgcaat gaagcgcagt atatgcaacg ttcatggact agcgcttata
      181 ctgcttatat tgtgggagac ggcggaattg tttatcaagt cggtcaacct ggttatgtac
      241 agtacggtgc tggttcgtat gctaatgcca acagtcctgt gcagattgag ttacaacaca
      301 cacatgataa agcaacgttt gagaaaaact acaaggcata cgttgaattg gctagagatt
      361 cagcaatgaa atatggtatt ccattaacgt tggacactcc ttataaccaa ccgggaatca
      421 aatcgcattt atgggtaaca caaaacatct ggggcgatca tacagatcct tacggttatc
      481 tttctgaaat gggcgtaagt aaagaaaaat tagcatatga tttagctcat ggatttaccg
      541 atgaaaatcc aacaacttct gaaaacaagc ctgtcattga tccaacacga gctggtgcag
      601 ctaatcctac actgacagat ggaacgaatt acgcccacat tgatcagttt ggagaaatcg
      661 aaaatgcaaa tttgcatgta gctggatggc acattgctaa ctataaatac gagtatatct
      721 tcattatgga ttacaatact gggaaagaat tagctcgagt aagagctgat ggaatttata
      781 gagcagatgt aaatcaagct tataatactt ctggaaatgt tggctatcat gtatctttta
      841 acatgcgtaa cttccctaat aagaaagtct atgtcatgat gcgggcaacg aatgatccag
      901 agggaaacac taaaggcggt gcgcaagatt tccatgacaa acgttggtat ttaaatattc
      961 ctaaacgata aaaatagctc ctcgttgagg agcattacat aactatattg acaactataa
     1021 aaatcattcg ataaaatagt gatgttatcg catatcttca ctatcaccca taatagtcac
     1081 actccaagct a
//
LOCUS       DAZKSR010000227         1059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000227 DAZKSR010000000
VERSION     DAZKSR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.239"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..162)
                     /locus_tag="ACG8XG_003096"
     tRNA            complement(89..162)
                     /locus_tag="ACG8XG_003096"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Ile,seq:cat)
     gene            complement(176..249)
                     /locus_tag="ACG8XG_003097"
     tRNA            complement(176..249)
                     /locus_tag="ACG8XG_003097"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Met,seq:cat)
     gene            complement(277..349)
                     /locus_tag="ACG8XG_003098"
     tRNA            complement(277..349)
                     /locus_tag="ACG8XG_003098"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Ala,seq:tgc)
     gene            complement(369..442)
                     /locus_tag="ACG8XG_003099"
     tRNA            complement(369..442)
                     /locus_tag="ACG8XG_003099"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Pro,seq:tgg)
     gene            complement(452..525)
                     /locus_tag="ACG8XG_003100"
     tRNA            complement(452..525)
                     /locus_tag="ACG8XG_003100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Arg,seq:acg)
     gene            complement(536..621)
                     /locus_tag="ACG8XG_003101"
     tRNA            complement(536..621)
                     /locus_tag="ACG8XG_003101"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(585..587),aa:Leu,seq:taa)
     gene            complement(638..709)
                     /locus_tag="ACG8XG_003102"
     tRNA            complement(638..709)
                     /locus_tag="ACG8XG_003102"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Gly,seq:gcc)
     gene            complement(721..793)
                     /locus_tag="ACG8XG_003103"
     tRNA            complement(721..793)
                     /locus_tag="ACG8XG_003103"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(758..760),aa:Thr,seq:tgt)
     gene            complement(811..892)
                     /locus_tag="ACG8XG_003104"
     tRNA            complement(811..892)
                     /locus_tag="ACG8XG_003104"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(856..858),aa:Leu,seq:tag)
     gene            complement(900..972)
                     /locus_tag="ACG8XG_003105"
     tRNA            complement(900..972)
                     /locus_tag="ACG8XG_003105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(937..939),aa:Lys,seq:ttt)
     gene            complement(976..1048)
                     /locus_tag="ACG8XG_003106"
     tRNA            complement(976..1048)
                     /locus_tag="ACG8XG_003106"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1013..1015),aa:Val,seq:tac)
BASE COUNT          272 a          305 c          250 g          232 t
ORIGIN      
        1 gcacggtttt acccgcctga cggttttcaa gaccgttccc ttcagccgga cttgggtaat
       61 cctccatatg aaacttacaa cgccacaatg gaccttgtag gactcgaacc tacgaccgga
      121 cggttatgag ccgtctgctc taaccaactg agctaaaggt ccaggctctt aaaaatcgcg
      181 gcggagggga tcgaaccccc gacctcccgg gtatgaaccg gacgctctag ccagctgagc
      241 tacaccgcga aaaagatatt cattatttaa atgaaatgga gcctagcggg atcgaaccgc
      301 tgacctcctg cgtgcaaagc aggcgctctc ccagctgagc taaggccccg tgtttaaatt
      361 tataattatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
      421 taccaagctg agctacttcc cgtttaatta acgcgcccaa gaggagtcga acccctaacc
      481 ttttgatccg tagtcaaaca ctctatccag ttgagctatg ggcgcatatt tgaaatgccg
      541 aggaccggaa tcgaaccggt acggtgatca ctcaccgcag gattttaagt cctgtgcgtc
      601 tgccagttcc gccaccccgg cgcgattgct ttggcaaagc ggaaaacggg gttcgaaccc
      661 gcgaccccca ccttggcaag gtggtgctct accactgagc tatttccgcg tgtttaaaga
      721 tgccggctaa aggacttgaa ccctcgaccc tctgattaca aatcagatgc tctaccaact
      781 gagctaagcc ggcatgtaaa acttaaatta tgcgggtgaa gggacttgaa cccccacgcc
      841 gtaaggcgct agatcctaaa tctagtgcgt ctgccaattc cgccacaccc gcaaaaatat
      901 gagccgtaca gggctcgaac ctgtgaccct ctgattaaaa gtcagatgct ctaccaactg
      961 agctaacggc tcatatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca
     1021 gatgctctcc cagctgagct aaacctccat ggataactt
//
LOCUS       DAZKSR010000228         1045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000228 DAZKSR010000000
VERSION     DAZKSR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.240"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8XG_003107"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8XG_003107"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0774932.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZKSR010000229         1004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000229 DAZKSR010000000
VERSION     DAZKSR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.241"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..>1004
                     /locus_tag="ACG8XG_003108"
     CDS             17..>1004
                     /locus_tag="ACG8XG_003108"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0774933.1"
                     /translation="MILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPFCFIS
                     KQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLLFDEF
                     RSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVTDMNA
                     PYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQLKSL
                     FKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLLTAYQ
                     EKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH"
BASE COUNT          301 a          209 c          191 g          303 t
ORIGIN      
        1 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
       61 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
      121 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
      181 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
      241 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
      301 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
      361 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
      421 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
      481 aacggatatg aacgctcctt atttctcact agttaaagag tgttttccaa atgcacaagt
      541 gatcgtcgat cggtttcaca ttgttcaaca tctgaaccgc ttcttcgatg cggttcgaaa
      601 acgtgtgatg aaaacacttg atcaaaaaga cccaatacaa gctaaacagt atcgccaatt
      661 gaaatcctta ttcaaattac ttttaaaatc agaggatcgt ttagattaca atacactaac
      721 gaaaagacgc aatttcaatt ggagactatt gactgaaaca gaagtagttg accgccttct
      781 aaagatttct cctgaactta aaacagctta tcgttactac caagacctgc ttactgcgta
      841 tcaggaaaaa gatccagata cttttttcca gctgcttcga gcaatgcctg gagaagttcc
      901 attggaactg caccatatca aaaaagcctt tttcaaatac gagaaaggga ttcggctggc
      961 attactgaag aagtattcga atgcacgact ggaaaatctc cata
//
LOCUS       DAZKSR010000230         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000230 DAZKSR010000000
VERSION     DAZKSR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.242"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACG8XG_003109"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACG8XG_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAZKSR010000231          998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000231 DAZKSR010000000
VERSION     DAZKSR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.243"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..634)
                     /locus_tag="ACG8XG_003110"
     CDS             complement(80..634)
                     /locus_tag="ACG8XG_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774934.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DAZKSR010000232          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000232 DAZKSR010000000
VERSION     DAZKSR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.244"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..264)
                     /locus_tag="ACG8XG_003111"
     CDS             complement(34..264)
                     /locus_tag="ACG8XG_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774935.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRCILEVETKFKNRSE"
     gene            complement(523..636)
                     /locus_tag="ACG8XG_003112"
                     /pseudo
     CDS             complement(523..636)
                     /locus_tag="ACG8XG_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          344 a          159 c          151 g          334 t
ORIGIN      
        1 accataattg cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt
       61 ctctacctct aatatacatc gagtttttat tttgccttca gagaatactg tttctttttt
      121 tgcagttaca ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat
      181 ctggaatttg tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt
      241 ttttctaata aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt
      301 ctttttgtaa aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa
      361 attatgtgcc aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga
      421 taacatttct tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac
      481 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
      541 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      601 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      661 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      721 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      781 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      841 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      901 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      961 agcttagatc ctttattcta tgctttat
//
LOCUS       DAZKSR010000233          970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000233 DAZKSR010000000
VERSION     DAZKSR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.245"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>970
                     /locus_tag="ACG8XG_003113"
     CDS             82..>970
                     /locus_tag="ACG8XG_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0774936.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANIN"
BASE COUNT          329 a          182 c          191 g          268 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc
//
LOCUS       DAZKSR010000234          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000234 DAZKSR010000000
VERSION     DAZKSR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.246"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8XG_003114"
     rRNA            <1..>943
                     /locus_tag="ACG8XG_003114"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKSR010000235          903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000235 DAZKSR010000000
VERSION     DAZKSR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.247"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          349 a          103 c          113 g          338 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttgataat aaagtactac
      601 ctattaaagg tagtacttta tttaatttag tataaattaa catactacta tattttaggt
      661 ttcatattat ataaattaat aatatactac catttgttgg tagtataatt taaaggtaat
      721 aagttattct tttattacta tgtttgggtt ttatagcaaa gaaagtttaa taagagtttg
      781 gacctagcaa acaatattaa tgaggtcatt aaatcttatt atctttagtg atataatgaa
      841 atatctacac gacaaaatta tgatcttgcg taaagttata aaatattaat aaaaatattt
      901 tat
//
LOCUS       DAZKSR010000236          891 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000236 DAZKSR010000000
VERSION     DAZKSR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.248"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..873
                     /locus_tag="ACG8XG_003115"
     CDS             250..873
                     /locus_tag="ACG8XG_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0774937.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          326 a          129 c          167 g          269 t
ORIGIN      
        1 ctaatgtcct tatttttgtg ttcacatttc tgttacctaa gaaatactta aaggaggtga
       61 tgtagccagc aacgactaca acttgaaccc gctatctaca gtttatttgt tgtttctttt
      121 gggtatactc taatggtaca aatccatatt tgcttaaagc gttgattttt cagcggtttt
      181 tctttattgc aaagtgtacc ctaagacctg tatactaata gtaaatagat tggataggaa
      241 tgatggcaaa tgtatttagc ttatttaaga gtttcttccg acgatcaaag tttagctcgg
      301 caacataagc taattgaaaa ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa
      361 attttcgagg aaaaagtctc tggaaaaaat attgaaaaca gaccagagct gcaagagctt
      421 atttctttca tacgtgaacg agatacgatt attattccta gtcttgatcg attagcaaga
      481 aatagtaaag atacaaaaga cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa
      541 atattagacc taccgtcgtt taatggggta actgaccctg cattaagaga cttgctaaca
      601 aatttagtca tagaagtgtt tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag
      661 cgtcaaaaac aaggcattga aattgcaaag aaaaatggtc gttatactgg taaaaagatt
      721 caataccatg ccaatgcaac aggaaaaaac aaattgattt acgatgaaat tgttgatggt
      781 ctgaagaaaa aagaaactat ttctaaaata gcaaagaacg caggagttac aagaaaaacc
      841 gtttatcgag taaaaagaga tttaggaatg taatatgttc cagttagaaa g
//
LOCUS       DAZKSR010000237          866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000237 DAZKSR010000000
VERSION     DAZKSR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.249"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>866
                     /locus_tag="ACG8XG_003116"
     CDS             <1..>866
                     /locus_tag="ACG8XG_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0774938.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVE"
BASE COUNT          377 a          112 c          140 g          237 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaata
//
LOCUS       DAZKSR010000238          855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000238 DAZKSR010000000
VERSION     DAZKSR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.250"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            413..855
                     /locus_tag="ACG8XG_003117"
                     /pseudo
     CDS             413..855
                     /locus_tag="ACG8XG_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          306 a          148 c          156 g          245 t
ORIGIN      
        1 tatttatcac ggatgagctt gaaatcgtct tttatacgta tcaaattgaa atgttcggca
       61 tacaacctat cgttgttact acattactaa ttgtcacttg ttaaggaact tggatccttt
      121 ctaaacattc cctagagata aattttactt tatccctccg ttaatatcca atttaagcac
      181 ctttccgatt cagggttaat ttgcatatca agtgcagctt gtgttctagt aagattagcc
      241 cttttagtcc ggctgagttg acttatggat ataagatatg gtcatagcaa atagatacta
      301 cgtatagtct gttaatttag tgtcattaat cttaagttgg ataggctaca attaactaga
      361 cagaaaaatt aaggtgtgta gactagaaga aaacatacca ggaggaattt ttatgtctaa
      421 gagaacacga agaacttttt cacaagaatt caagcaacaa atcgtcaatc tttacttagc
      481 tggaaagcca cgtgtagaaa tcattcgaga atatgaacta acggcttcag catttgacaa
      541 atgggtaaag caatctaaaa cgagtggttc attcaaagaa aaagataatc ctacgcctga
      601 acaaaaagaa ttgttagaac tacgtaaaag aaaccagcaa ttagaaatgg aaaatgatat
      661 tttaaagcaa gcagcgctga tattcggacg aagagacaag taatcgatgc gaataagcat
      721 ctttacccta tatcagcgat gtgcagaata ttaggtctat cacgtcagtc ctattattat
      781 caatcaaaac caaagaaaga cgaatcagaa cttgaagaag cagtcgctga agaatttatc
      841 cgcagccgaa aggcc
//
LOCUS       DAZKSR010000239          793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000239 DAZKSR010000000
VERSION     DAZKSR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.251"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..678
                     /locus_tag="ACG8XG_003118"
     CDS             <1..678
                     /locus_tag="ACG8XG_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0774939.1"
                     /translation="HSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKI
                     DNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQR
                     TDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYG
                     RKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLR
                     LHSMGLA"
BASE COUNT          291 a          124 c          146 g          232 t
ORIGIN      
        1 cattctagcg gtaaatacta tatttctttg ttgtgtaaag aagaaattat cgaattacct
       61 aaaactaatt ccacaattgg tattgactta ggtattatgg actttgcgat tctttctgat
      121 ggacaaaaaa ttgataataa taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa
      181 caacgcaaat tgtctaggcg tgctttattg gctaaaaata aaggtaccaa tctatttgaa
      241 gctaaaaatt atcaaaaaca aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat
      301 caacgtactg attttctgaa taagttgagt acagaaatga tcaaaaatca cgatattatc
      361 tgtattgaag acttaaacac aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt
      421 tctgatgttt cttggtcaaa gtttgtgact aaattacaat acaaggctga ctggtatgga
      481 cgaaaaatca tcaaagtaga taaatggttt ccgtctagtc agatttgttc agagtgcgga
      541 cataaagatg gcaagaaatc tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact
      601 catcatgatc gtgatatcaa tgctagtatc aatattctga ccgaaggtct aagactacat
      661 tctatgggat tggcttagat ccaatcatca agaaccgtag gaactacggg gatagcttgg
      721 taaataagag acaccgctgt tagtaaagaa atacgctatc aagtatgctc tattcccaag
      781 aagctcccac ttc
//
LOCUS       DAZKSR010000240          787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000240 DAZKSR010000000
VERSION     DAZKSR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.252"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG8XG_003119"
     CDS             <1..716
                     /locus_tag="ACG8XG_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0774940.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DAZKSR010000241          714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000241 DAZKSR010000000
VERSION     DAZKSR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.253"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>714)
                     /locus_tag="ACG8XG_003120"
     rRNA            complement(<1..>714)
                     /locus_tag="ACG8XG_003120"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          145 a          224 c          152 g          193 t
ORIGIN      
        1 atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac gctttcgagc
       61 ctcagcgtca gttacagacc agagagccgc cttcgccact ggtgttcctc catatatcta
      121 cgcatttcac cgctacacat ggaattccac tctcctcttc tgcactcaag tctcccagtt
      181 tccaatgacc ctccccggtt gagccggggg ctttcacatc agacttaaga aaccgcctgc
      241 gctcgcttta cgcccaataa atccggacaa cgcttgccac ctacgtatta ccgcggctgc
      301 tggcacgtag ttagccgtgg ctttctggtt agataccgtc aagggatgaa cagttactct
      361 catccttgtt cttctctaac aacagagttt tacgatccga aaaccttctt cactcacgcg
      421 gcgttgctcg gtcagacttt cgtccattgc cgaagattcc ctactgctgc ctcccgtagg
      481 agtttgggcc gtgtctcagt cccaatgtgg ccgatcaccc tctcaggtcg gctatgcatc
      541 gtggccttgg tgagccgtta cctcaccaac tagctaatgc accgcgggtc catccatcag
      601 cgacacccga aagcgccttt caaatcaaaa ccatgcggtt ttgattgtta tacggtatta
      661 gcacctgttt ccaagtgtta tccccttctg atgggcaggt tacccacgtg ttac
//
LOCUS       DAZKSR010000242          649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000242 DAZKSR010000000
VERSION     DAZKSR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.254"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACG8XG_003121"
     CDS             <1..>649
                     /locus_tag="ACG8XG_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0774941.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAZKSR010000243          642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000243 DAZKSR010000000
VERSION     DAZKSR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.255"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..541)
                     /locus_tag="ACG8XG_003122"
     CDS             complement(242..541)
                     /locus_tag="ACG8XG_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0774942.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          215 a          113 c           72 g          242 t
ORIGIN      
        1 ttttaactta caaaaatgca acattaataa ataacgatag tttcataaaa tacactttta
       61 attaacatta ttagtttata ctagctgttt ttgtgttaat taaaggataa aatacaatat
      121 aaagaaacat ctgtttcttt atttagaata aaaaaataca gactctgttc aaataaatga
      181 acctagcctg cattatgaga ttgactatac tttttaattc attgagatga ataactcgtt
      241 tttataaatg cccaaaagta atcggtgccg aagccattcc tcgacctaat tctttttcaa
      301 attttcctat tgttgatatc tccatataaa acggtgatac tggtttagac aattctcccc
      361 tgattgctaa cggacgtaaa ctacgttcca aattatttat gactttagga taatactgac
      421 ctttttctaa gcctgtcttt gcgtctatca aaagtatacg ttccttttct ttgatctctg
      481 gatttaaaac gagatcatat aattttagta ccatgttttc tatttcttca cttttagaca
      541 tcacgatttc ctcccaaaaa tatatttctt cccactacat tctataaaca aagtatacgc
      601 tacatctaga tttttttaaa tcattactgg attgttaaaa cc
//
LOCUS       DAZKSR010000244          573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000244 DAZKSR010000000
VERSION     DAZKSR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.256"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          185 a           81 c           81 g          226 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct ttt
//
LOCUS       DAZKSR010000245          533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000245 DAZKSR010000000
VERSION     DAZKSR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.257"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..380
                     /locus_tag="ACG8XG_003123"
     CDS             45..380
                     /locus_tag="ACG8XG_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774943.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          207 a           63 c           97 g          166 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtgcgattt ctattttatt
      481 atatcaaaat aaagagagat tctaaagaaa gaagagggaa atatataaat caa
//
LOCUS       DAZKSR010000246          531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000246 DAZKSR010000000
VERSION     DAZKSR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.258"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..291)
                     /gene="tnpB"
                     /locus_tag="ACG8XG_003124"
     CDS             complement(<1..291)
                     /gene="tnpB"
                     /locus_tag="ACG8XG_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0774944.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWL"
     gene            complement(281..475)
                     /locus_tag="ACG8XG_003125"
     CDS             complement(281..475)
                     /locus_tag="ACG8XG_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774945.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          155 a          112 c           92 g          172 t
ORIGIN      
        1 caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc gaggccattt
       61 taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt cccaatagag
      121 aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt cgtaaagctc
      181 gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc gcaaatctgt
      241 ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca ttggacaaca
      301 gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc aaaacttcag
      361 aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta cctttttctg
      421 gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta tacatagaaa
      481 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag t
//
LOCUS       DAZKSR010000247          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000247 DAZKSR010000000
VERSION     DAZKSR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.259"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..>523
                     /locus_tag="ACG8XG_003126"
     CDS             27..>523
                     /locus_tag="ACG8XG_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0774946.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          191 a           96 c           86 g          150 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DAZKSR010000248          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000248 DAZKSR010000000
VERSION     DAZKSR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.260"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..>513)
                     /locus_tag="ACG8XG_003127"
     CDS             complement(107..>513)
                     /locus_tag="ACG8XG_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0774947.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          102 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttc
//
LOCUS       DAZKSR010000249          508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000249 DAZKSR010000000
VERSION     DAZKSR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.261"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          227 a           70 c           81 g          130 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactct
//
LOCUS       DAZKSR010000250          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000250 DAZKSR010000000
VERSION     DAZKSR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.262"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..140
                     /locus_tag="ACG8XG_003128"
     CDS             <1..140
                     /locus_tag="ACG8XG_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFQ0774948.1"
                     /translation="VDAATYYGNGLYCNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGG
                     R"
     gene            140..490
                     /locus_tag="ACG8XG_003129"
     CDS             140..490
                     /locus_tag="ACG8XG_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0774949.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          202 a           55 c           85 g          159 t
ORIGIN      
        1 acgttgacgc agctacttac tatggaaatg gtctttattg taacaaagaa aaatgttggg
       61 taaattgggg acaatcatgg tcagaagggt taaaacgttg gggagataat ctttttggta
      121 gttttagtgg aggacgataa tgacttcaat aaacattgat cacacaaaaa aagtcataca
      181 tgaattatac aatagtttaa tgacacgacc taatcagacg aaagcattat tagatattac
      241 tgatgtttta tttcaggtat ataaaaaaat tgatacagca aaatatcctg aaattcttgt
      301 aaataaatta gttaattata tttacatcgt gggttttgat aataaaatac gctttattga
      361 taatgatgaa aaattattaa tagaattagg ggatatttct aaaaaagctg gaattaatag
      421 taaatacaaa gcaaacttta cagataaatc gcaattttat agttattcag aaacaatgcc
      481 tcaacgataa aatatagtat a
//
LOCUS       DAZKSR010000251          498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000251 DAZKSR010000000
VERSION     DAZKSR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.263"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..498
                     /locus_tag="ACG8XG_003130"
                     /pseudo
     CDS             258..498
                     /locus_tag="ACG8XG_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          169 a           91 c           69 g          169 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttcc
//
LOCUS       DAZKSR010000252          496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000252 DAZKSR010000000
VERSION     DAZKSR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.264"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..327
                     /locus_tag="ACG8XG_003131"
     CDS             <1..327
                     /locus_tag="ACG8XG_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774950.1"
                     /translation="KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQ
                     REGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRK
                     LSEVNN"
BASE COUNT          199 a           66 c           90 g          141 t
ORIGIN      
        1 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
       61 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
      121 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
      181 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
      241 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
      301 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
      361 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
      421 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
      481 aattcggccc tataat
//
LOCUS       DAZKSR010000253          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000253 DAZKSR010000000
VERSION     DAZKSR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.265"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..>493)
                     /locus_tag="ACG8XG_003132"
     CDS             complement(54..>493)
                     /locus_tag="ACG8XG_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774951.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DAZKSR010000254          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000254 DAZKSR010000000
VERSION     DAZKSR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.266"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..395)
                     /locus_tag="ACG8XG_003133"
     CDS             complement(<1..395)
                     /locus_tag="ACG8XG_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774952.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVP"
BASE COUNT          134 a           79 c           76 g          182 t
ORIGIN      
        1 ggaactttga tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta
       61 taactctgaa caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga
      121 aaaaaattct tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca
      181 atttctttca aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt
      241 ttagaagaat catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg
      301 tagaccttac ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga
      361 tagattctga atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg
      421 attgtttcct tgattttgga tatacttctt aatcacatca attggtgcgc c
//
LOCUS       DAZKSR010000255          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000255 DAZKSR010000000
VERSION     DAZKSR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.267"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>470
                     /locus_tag="ACG8XG_003134"
     CDS             <1..>470
                     /locus_tag="ACG8XG_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFQ0774953.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DAZKSR010000256          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000256 DAZKSR010000000
VERSION     DAZKSR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.268"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8XG_003135"
     CDS             complement(<1..430)
                     /locus_tag="ACG8XG_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0774954.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZKSR010000257          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000257 DAZKSR010000000
VERSION     DAZKSR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.269"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>455
                     /locus_tag="ACG8XG_003136"
     CDS             <1..>455
                     /locus_tag="ACG8XG_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774955.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV"
BASE COUNT          150 a           95 c           90 g          120 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct
      421 ctaaaggagg cgtatccctt ttttcatgaa ttagt
//
LOCUS       DAZKSR010000258          434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000258 DAZKSR010000000
VERSION     DAZKSR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.270"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>434)
                     /locus_tag="ACG8XG_003137"
     CDS             complement(<1..>434)
                     /locus_tag="ACG8XG_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774956.1"
                     /translation="QTIKEVDTNVAYRWFLGYSFEEKIPHFSTFGKNYVRRFRETTVF
                     EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEI
                     NRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          107 a           95 c           77 g          155 t
ORIGIN      
        1 ctcgttcgcc tttgacgtag tagccgcttt ccggatcagc tttacttatt tttctttctt
       61 tcagcttcac ttccttttct aagtccaaag taaaagggcg ttttccagcc tctattcgtt
      121 gacgattgat ctcatcttct aattcatcct gataggcttt cgcttcatca tgggtcattt
      181 ctttcgtaaa cttatgttta ttggcgttag ctttgatgtg ggtagaatcc agatacaaat
      241 tatcctctgt tacaaaacct gctttaactg cttgttctaa aatataggca aaaatatcct
      301 caaacaccgt cgtttctcta aatcggcgaa cataattctt tccaaaagtt gagaaatggg
      361 ggattttctc ctcgaaggag tagcctaaaa accagcggta agcgacattg gtatcgactt
      421 ccttaatggt ttgt
//
LOCUS       DAZKSR010000259          406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000259 DAZKSR010000000
VERSION     DAZKSR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.271"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>406
                     /locus_tag="ACG8XG_003138"
     CDS             <1..>406
                     /locus_tag="ACG8XG_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0774957.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGL
                     PKEMEFNQVNQGFISSVASKRNHIPRKSLNYQT"
BASE COUNT          147 a           90 c           67 g          102 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaagat
      301 ggactaccaa aagaaatgga attcaaccaa gtcaatcaag gattcatctc atccgttgcg
      361 tccaaaagaa accatatccc tagaaaatca ctaaattacc aaacac
//
LOCUS       DAZKSR010000260          406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000260 DAZKSR010000000
VERSION     DAZKSR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.272"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..58)
                     /locus_tag="ACG8XG_003139"
     tRNA            complement(1..58)
                     /locus_tag="ACG8XG_003139"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31..33),aa:Ala,seq:tgc)
     gene            complement(143..>406)
                     /locus_tag="ACG8XG_003140"
     rRNA            complement(143..>406)
                     /locus_tag="ACG8XG_003140"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          102 a          111 c          100 g           93 t
ORIGIN      
        1 tagcgggatc gaaccgctga cctcctgcgt gcaaagcagg cgctctccca gctgagctaa
       61 ggccccgttt taaattgaga gtaaacctct caaaactgaa caaagtaaaa acaaattgtg
      121 tagtctccgt aatattcctt agaaaggagg tgatccagcc gcaccttccg atacggctac
      181 cttgttacga cttcacccca atcatctatc ccaccttagg cggctggctc caaaaggtta
      241 cctcaccgac ttcgggtgtt acaaactctc gtggtgtgac gggcggtgtg tacaaggccc
      301 gggaacgtat tcaccgcggc gtgctgatcc gcgattacta gcgattccgg cttcatgcag
      361 gcgagttgca gcctgcaatc cgaactgaga gaagctttaa gagatt
//
LOCUS       DAZKSR010000261          399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000261 DAZKSR010000000
VERSION     DAZKSR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.273"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          164 a           43 c           69 g          123 t
ORIGIN      
        1 aataaataaa aatagggaat tttctattgc aattttataa agagattgct atattattat
       61 ttgttctaaa tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac
      121 ttttgatgtc atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt
      181 ttatcgaaaa aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc
      241 aagtcgctgc taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca
      301 ttctcttctg aatctgataa gattaaagag ttgttaaaac aaaaagatta gacctttgaa
      361 aactgaacaa agtaagacaa accaaatgtg tagggcgtc
//
LOCUS       DAZKSR010000262          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000262 DAZKSR010000000
VERSION     DAZKSR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.274"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>391
                     /locus_tag="ACG8XG_003141"
     CDS             <1..>391
                     /locus_tag="ACG8XG_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774958.1"
                     /translation="NLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSV
                     NFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAV
                     VFYGISNNLILSYVLYIWLLNICTILIS"
BASE COUNT          144 a           48 c           48 g          151 t
ORIGIN      
        1 aacttattaa aaaaagacaa ttctagtata ctgaaaatag aattaaaaaa ggagaacaac
       61 aaatctatga aaataagtaa aaaagcattt cttttagtag ttcttatctt acttagtact
      121 ttatattcag taaattttat gagaaatgct caagaaattt atacaacagg tgatttatct
      181 tttcatcttt cacgaatcaa agggttatct tctatttttg aagggccaat caactataca
      241 acatttaaca attatggaga tggactgaac tatttttatc cttttcttac aataattcct
      301 gcagtcgttt tttatggaat ttcaaataac ttaatattaa gttatgttct atatatttgg
      361 ttattaaata tttgtacgat tctgattagt t
//
LOCUS       DAZKSR010000263          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000263 DAZKSR010000000
VERSION     DAZKSR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.275"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>378
                     /locus_tag="ACG8XG_003142"
     CDS             <1..>378
                     /locus_tag="ACG8XG_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0774959.1"
                     /translation="AKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQIND
                     LYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKY
                     KERDIYINECIEIVDSLTKNISQ"
BASE COUNT          179 a           33 c           48 g          118 t
ORIGIN      
        1 atgcaaaatc aataaaaaat aatatacaag aaataaaaat tgttactgat aaaatgatga
       61 aacttgataa aaaaatgagt ttaaaagtta gctctaatga tgaagttgga gaattaaaac
      121 aacaaattaa tgatttgtat tctactttat taagaacaat tgacgatttg gaatttaaaa
      181 ataaagaaat tttaaaatta gaaaagttaa aatatgactt ttttaaaggt gcatcccatg
      241 aacttaaaac ccctcttgct agtcttaaaa taatactgga aaacatgaaa tacaatattg
      301 gaaaatataa agaaagagat atttatatta atgaatgtat tgaaatcgtt gatagtttaa
      361 caaaaaatat ttctcaaa
//
LOCUS       DAZKSR010000264          374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000264 DAZKSR010000000
VERSION     DAZKSR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.276"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>374
                     /locus_tag="ACG8XG_003143"
     CDS             82..>374
                     /locus_tag="ACG8XG_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0774960.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          133 a           62 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa ccca
//
LOCUS       DAZKSR010000265          372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000265 DAZKSR010000000
VERSION     DAZKSR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.277"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>372)
                     /locus_tag="ACG8XG_003144"
     rRNA            complement(<1..>372)
                     /locus_tag="ACG8XG_003144"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           83 a          107 c           91 g           91 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt ta
//
LOCUS       DAZKSR010000266          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000266 DAZKSR010000000
VERSION     DAZKSR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.278"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8XG_003145"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8XG_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0774961.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKSR010000267          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000267 DAZKSR010000000
VERSION     DAZKSR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.279"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8XG_003146"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8XG_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0774962.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKSR010000268          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000268 DAZKSR010000000
VERSION     DAZKSR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.280"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8XG_003147"
     tRNA            complement(20..91)
                     /locus_tag="ACG8XG_003147"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8XG_003148"
     tRNA            complement(113..200)
                     /locus_tag="ACG8XG_003148"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8XG_003149"
     tRNA            complement(225..298)
                     /locus_tag="ACG8XG_003149"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
BASE COUNT           84 a           82 c           68 g           66 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
//
LOCUS       DAZKSR010000269          297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000269 DAZKSR010000000
VERSION     DAZKSR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.281"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           33 c           46 g          113 t
ORIGIN      
        1 gactacctgt attagtcctc ttttttgacc agattttgac cagtctattt tacattctaa
       61 tatattcata tgagctaatt ttatgattaa atggtaagtt tttccttgtt tgttttagtt
      121 taaactagtg tgtaattcaa aaatctatat ggaaggatct gtggggtaaa tcattataca
      181 aaagtaaaat tttacttatt aacaggctgt agaaatataa aaaagaacta aaatttaact
      241 aaattatgtg ctattcttaa ggaaagccaa gtgtatgaga aagtttctga gaatata
//
LOCUS       DAZKSR010000270          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000270 DAZKSR010000000
VERSION     DAZKSR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.282"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           51 c           43 g           96 t
ORIGIN      
        1 tctataattt ctaggactga tggaaatttt ctatctgtcc tagttttttt gtaaaaaaac
       61 aaaaagacat acaaaatttc cggtagaata gagtctccta caacatcatt ctgggggaat
      121 tcttgtatgt ccatgaatca ttttatcaaa caactcttta ctattaaaga ctcaaatatc
      181 caaattcaat cctttgaaga aactttttat aaaagcagaa aaagtatcgt tattgaggga
      241 actctgatcc gtacctacag acgttgtccg tgttgtaaag attctacaaa gcaaa
//
LOCUS       DAZKSR010000271          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000271 DAZKSR010000000
VERSION     DAZKSR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.283"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACG8XG_003150"
     CDS             <1..185
                     /locus_tag="ACG8XG_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0774963.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAZKSR010000272          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000272 DAZKSR010000000
VERSION     DAZKSR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.284"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8XG_003151"
     CDS             <1..>291
                     /locus_tag="ACG8XG_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0774964.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVG"
BASE COUNT          119 a           40 c           70 g           62 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg g
//
LOCUS       DAZKSR010000273          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000273 DAZKSR010000000
VERSION     DAZKSR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.denovo.285"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>290
                     /locus_tag="ACG8XG_003152"
     CDS             <1..>290
                     /locus_tag="ACG8XG_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0774965.1"
                     /translation="YEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTD
                     VNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVS"
BASE COUNT          124 a           15 c           49 g          102 t
ORIGIN      
        1 tatgaaatat tagcaaatca aaagaaaata actgtcaata attatatatt agacgagaat
       61 gtttatatag gaaaaacagc tttaaagatt attctatcta atttgattag taatgcggta
      121 aaatatactg atgtaaatgg ggtaattaat attgggatag ttaatgattg gttatatata
      181 gagaattcat acggtaacaa taaaatttcg aatatggata aaatatttga tgttaaattt
      241 gatttaaata aggaaaatag taatggatta ggtttatata ttgtaagtaa
//
LOCUS       DAZKSR010000274        12501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.090, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000274 DAZKSR010000000
VERSION     DAZKSR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.090"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..479)
                     /locus_tag="ACG8XG_003153"
     CDS             complement(<1..479)
                     /locus_tag="ACG8XG_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0774966.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     "
     gene            complement(684..1877)
                     /locus_tag="ACG8XG_003154"
     CDS             complement(684..1877)
                     /locus_tag="ACG8XG_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0774967.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1956..2162)
                     /locus_tag="ACG8XG_003155"
     CDS             complement(1956..2162)
                     /locus_tag="ACG8XG_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0774968.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2155..3297)
                     /locus_tag="ACG8XG_003156"
     CDS             complement(2155..3297)
                     /locus_tag="ACG8XG_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0774969.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3809..4444
                     /locus_tag="ACG8XG_003157"
     CDS             3809..4444
                     /locus_tag="ACG8XG_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0774970.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4580..4672
                     /locus_tag="ACG8XG_003158"
                     /pseudo
     CDS             4580..4672
                     /locus_tag="ACG8XG_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4786..5421
                     /locus_tag="ACG8XG_003159"
     CDS             4786..5421
                     /locus_tag="ACG8XG_003159"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0774971.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            5528..6766
                     /gene="mef(H)"
                     /locus_tag="ACG8XG_003160"
                     /gene_synonym="mefH"
     CDS             5528..6766
                     /gene="mef(H)"
                     /locus_tag="ACG8XG_003160"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFQ0774972.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            6993..7412
                     /locus_tag="ACG8XG_003161"
     CDS             6993..7412
                     /locus_tag="ACG8XG_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0774973.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            7417..7656
                     /locus_tag="ACG8XG_003162"
     CDS             7417..7656
                     /locus_tag="ACG8XG_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0774974.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(7881..8804)
                     /locus_tag="ACG8XG_003163"
     CDS             complement(7881..8804)
                     /locus_tag="ACG8XG_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0774975.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(8836..9600)
                     /gene="map"
                     /locus_tag="ACG8XG_003164"
     CDS             complement(8836..9600)
                     /gene="map"
                     /locus_tag="ACG8XG_003164"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0774976.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            9758..10198
                     /locus_tag="ACG8XG_003165"
     CDS             9758..10198
                     /locus_tag="ACG8XG_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0774977.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(10376..10945)
                     /locus_tag="ACG8XG_003166"
     CDS             complement(10376..10945)
                     /locus_tag="ACG8XG_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0774978.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            11200..12432
                     /locus_tag="ACG8XG_003167"
     CDS             11200..12432
                     /locus_tag="ACG8XG_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0774979.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         3844 a         2284 c         2345 g         4028 t
ORIGIN      
        1 ccgtcaatca gattgaccgc attcgtgata ccgaagatcc aaaaaatcgt tacgggaaaa
       61 ctaagccagc ctagttggat atatccaata aaaggaagcg tgaaggaatc aatacgtata
      121 tctgctacaa aataaataat aagagaagca atcaagatcc ctatactttt ttgtttaggt
      181 gtcagctcat atttatcatc gagcactcct gtgactatga ttactaaaga agcaatcaag
      241 atcggtacaa tatacgattg agggataatt ttacggaata agaataaagt cgatataaca
      301 aatgaaataa agataggcag tccgcccgct gaaggcattg gaactttatt tattcttctt
      361 tcatttggtt tgtcgaccat acctgtttgt actgaaatga ttttgaatac aggtgttaat
      421 atcagtgata ataaaattgt cagaaaaaat cttataacca tactaaaaga aacaagcata
      481 tgggcaccta ctttaattaa gagtcataat tattataaat tatatatgtc tttttgacta
      541 tctttttata tcttctcaaa tgtccgcaaa gccttatttt atcggctcta cgggcatttt
      601 gcttttgtgg taaacctcgc atatctaggt ctatcttctt atattttccc tgtcaatcgt
      661 ggttaaaatc gtggtaaata cttctatgcg attagacgct gaacctctga ttttgcggta
      721 tctatggacg catgagcgta ccagttcatt gtgatactga tgtttgaatg ccccatgata
      781 tactgtaaat ctttcgggtt catgttcttg cttgccagcc ttgtgcagaa tgtatggcgt
      841 agcgtatgcg gtgtaatatg tggcaaggga ttgtccttgt ggtgcttgtt gtatttcttt
      901 accatacgga taaataaggc gttataatca atggcaactt ttggattacc tttcggattg
      961 ataaacacaa aaccgctgtg tccgtctacc tctatcgact ttatctttgg tcggctgttg
     1021 ataacccgtt gaaatgcctg tatcgtttct ttgcttaatg gcacttgcct tatcccgctt
     1081 ttcgtcttag gcgtttcaat ataatagccc tgttccttac tctttagtaa ctggtggtcg
     1141 ataatcacaa cttcattctt gaaatcaatg tcggctactg tcagtccgca cagttccgag
     1201 atacgaagtc ctgtctttaa cagtatcagc acatcatcat aatgcttgtg atacacgttg
     1261 tccgtcttaa taaatgacaa taaggcttgc tcctgttcct ctgtcaatgc gactttctct
     1321 ttggtatcat tttctatgac ttcacttagc ttgaaatcaa aagggttttt cctcacataa
     1381 tcatcttgta tggctatgta gaatgacgct tttaacgagc gtttatggtt gttaatggta
     1441 ttgtaggaaa agcctttgtc tttcatgcgt aacgcccatt ctttagcgtc agagggtttt
     1501 atcgtatcaa tgctcctagc acctaacttg tcctctttta ataaccgcat gagttgttcc
     1561 cgttgtttct gtgtgctttt cttcacattt gtcctctgtg cgttctgttt ggcatagagt
     1621 tggcaaagcg tcattttctt gcctgtgctg tcgataccgt cctcaatatc ccgtcttatc
     1681 tgctgttcca gttctcgaag tgagggtttt tcccgttttc cctttggtgt cgggtctgtg
     1741 ggtgtcaatc tccaagcata cacatatttt gtgtttccca atgcgtctac atatttatat
     1801 aagtattttc cgtctgttcg ttggctctct ccagtatgca ggatacgacc tttgttatcc
     1861 cgtctttttt ctttcatggt gtctgctcct ttccttgttg gaaagagcct tgatatgact
     1921 tgtgttcatc ataacacata caaggctcat ttgcattaga ttgcgtccaa tttatcaata
     1981 accttttcaa actgtcggcg tttaatctgt atgcggttgc cattcataat gagccagcca
     2041 gcgtccttgt tttcctctgc caatcgtctt aacttgtttt ctccaatgcg gaaatacttt
     2101 gacgcttctt caatggtaag ggtgtatttt tcccatacgg gaatatcctg tgtattactc
     2161 ataaaacacc ccctcacttt cgattacgtc tgatatgtcc gtacacatct gttccacaag
     2221 catttcctgt ttttcagaaa ggtgggtaca ttcatcaatc attttcatgt agtttgaacc
     2281 tgtcagcctg tcgatttcct ctatcatcac gattgacggc atgacctgtc tttccagcca
     2341 ttgcagagtg cgttccatat tgacaggtac agggttcatt tcaaaaggta aactgtcatg
     2401 ggaaatgaat atctcccaca atggatagtc aggaaattcc acaaaatcgg taatgagata
     2461 aaacaccagc ccatgagggt tatacgtcaa taacagttct tcaactgcct gtaccgcctt
     2521 tttgtcacgc agacgaattt caaaacggtt gataatatcg gtatgtttct ttttcgttgc
     2581 ctgttccttt tgtttttcat acaggcagaa atattttgtg cttgcctttg agccgatata
     2641 aagggtactt gctaaattac ggttttttcc gcttaatttt ccaccttgta cattttcata
     2701 gtttttacag cgacattccg caccaccatt tctgtatttt tcagatagca caggaatatc
     2761 cagcagaccg cacatatcat tgattgccaa gtcaaaacgt cggataacac cgccacagtc
     2821 aagacagcgg tttaagaatg aataccagtc cctgttctgt gcctgtaaga catattccat
     2881 gttgcgtgag cctgcccctt ttaattctaa aaatacgccc atgtgttcat catcagaagc
     2941 catgaccttg atttccccgt aagcataatg ctccttatag ccataatacc catagtcata
     3001 atggataaaa taatctgctt tcatgccaag cacctttcgg ataacttcca atgcgtcagt
     3061 cgttggaaaa cggacagaca gataatcaac gagcagataa aaaggcttat cgcagatata
     3121 gtaattcaga caacgcagga taatgttctg tgtttccttg ctggcgttag cttttccgtt
     3181 ctcaaagcgg ttgatactat atcttgatac atgggtaaga cttgcaagtt ctccttgtga
     3241 aagaccgctg gcaagacgtt tgttcttcat ttcaagtgca aattctaaat tgttcattgt
     3301 attcctccaa tcccacaata gccttgaaat acctgtaaac cacctttcaa aaagtgtgat
     3361 atgtactatc ttcttaaaac ctctcattac tactcacagg tttgggggtg tatggttttt
     3421 gacttgtctt ttgtgaaata gtgcccccct gttagataac gggggcacag acaaggctcg
     3481 caggctcgcc agccgacaaa gcatttcttc cccttgacac aaggtgtcat ggcggtaagc
     3541 ctgagaggct tccgccaccg tgtcagaaat actttgtcag caagttctgt tttgcctttt
     3601 ctaacaagtt ccatttcaac cgatattcat atctacggat ttaatcagat aggtatttct
     3661 ttttgaatgt ttttatattt cattctattg tgcttattcg attacaagaa catagtaatt
     3721 cttatatgct tatttgtcaa tatctttttt tgcctttaca aatcatatta agcatgatag
     3781 acttataata gcgaagatat aacgagatat ggagggttat gcaatgaaaa taagccattt
     3841 aggaaataat atacagacca taagaaaatt taggggaatg aaacaacagg aacttgcgga
     3901 caaaatcggt atcaatatgc agagcctttc caagattgaa agagggttga actatcctgc
     3961 ctatgaaacg ctggaaaaga taatggaagt gctggacgtt acgcccaatg aattgttgtc
     4021 gggagaatgg aagtatgtca atcaatctga aaaggaagtc tgtcagtttt taagagccga
     4081 agaacgtcta aatgcagaac tgaaacatgg acactatgat aacttctttg acagcgagga
     4141 agaatggctg gaatatgagt tagaaaagtt acgggaatat attacagact atatcaacgg
     4201 gaaaagcatt gaagcgtccg acctttaccc aatcaaagaa tttatccagc atttgaagtt
     4261 tcaaaaatta ttagaccgct atgatgattt atacagtatg gatatgtttg gggaaagcat
     4321 agagggacat aagtataaga caccttatca agtcgtgaaa atgataaacc cgaactcaaa
     4381 agaagatatg gaactgttac gagaagtatt aaggaataac aattttgatg atgaggacga
     4441 gtaacttttt tcctcttgcg agaagataca gcaaacaaaa agcccgatca agagtgcaaa
     4501 gcaccaccaa tggtggcaaa gctcttgaca gggctttttc ttttctgttg tgggcaatca
     4561 agaggaagaa agggataaaa tctgatttgt ggtaaatcag gcaaaggcaa cgcaggtatc
     4621 acagtatgaa ttgacaccgc ataaggaaag caactggaag attgtagaat aaatgaaaaa
     4681 ggcttgcatt ctgcattttt ataaaattgc aagtctttat attgacaagc ataaaatact
     4741 aagatatact ttaatcagac caacggtttg tattggaggt aagttatggc acgaaataaa
     4801 tatccagaag aaacagtaaa actcatttta gatgtttcta caaaaatgtt ttgtgaaaaa
     4861 ggatatgata acacatcatt gcaagatatt atcaatgaaa caaaactttc taaaggtgct
     4921 atttatcatc atttcgattc aaaggaagat attttaaaag caatttttca aagaattgga
     4981 aatgaaaatg cagtaatatt tgctaaaatt agagataacg atagtttaaa cggtttagaa
     5041 aagttaagag aaatatttaa gacagcggta ttcaattcta accaaagcgt tttattgaca
     5101 gtttctcctc aactattaaa caatcctcgt tttcttgcta tgcaaataga acaaatgtat
     5161 gaaattgtag tacctgaatt tgttgaaccc gttttagaac aaggtatcaa ggataagtct
     5221 ttggagattg aaaattcaca tgaaattgca gaagttatca tggtattaag taatgtatgg
     5281 ctcaacccat tagttgaaat gacggataaa gagggtatgg aaaaacgttg caatacattc
     5341 aatgcccttt taaatggaat aggcatagac acattattag acgaagaaac aatttctgca
     5401 tttatacagt tttgtaaata actgtatttg ttatccctat acgatagact gctctataaa
     5461 agagcagttt atttaaacca ttatataaac cgacggtctg tttgattaaa taaaaaggag
     5521 agattttatg caagcagaaa aaaaactttt taatcgtgat tttatacttg ttgttatagg
     5581 acagataatt tctttatttg gaaacgctat cctacgattt gcattaccgt actatttgtt
     5641 aaaagaaact ggttcatcag ctttgtttgg tattgctaca gcctgctctt ttttacctat
     5701 ggttattttg tcgctggtag gtggtattat tgctgacagg acaaataaaa gaaacattat
     5761 ggtcatacta gattttgtaa cagcaatcat aattttagta ttttatttgt cattgggaaa
     5821 aattcctatc atacctttgt ttattgttgc tttgatgtta ctttatggta tttctggtgc
     5881 atatcaacca gcggtacaag caagtattcc tctattggta tcatcagata aacttaccgt
     5941 cggtaatgct ataataaatc aagtaaatac tttagcaaac tttatcggac cagctataag
     6001 cggagtagtt tttcaatttt atggtatcaa tcccatttta attgttagta ttatatgttt
     6061 tactttttca gccattatgg aaatttttat ccatattcca caccaaaaaa gacctcgtga
     6121 agtaggtgta attgcagttg ctaaaaacga tttaaaagat agttatacct ttttgaaaaa
     6181 ggagaaaccc atttttatat ccgtaatctt tttggtttgc attttcaatt tggttttaag
     6241 tgctgtttta attgttggtc tgcctatctt aatttctgaa gttttaaaaa tgccagatac
     6301 actatatggt ttttcacaat cagcattatc attaggtggt ttatgtggtg gtattttaac
     6361 agctcttgtt gcttcaaagt taaaactaaa aaacctttat accttgttac ttatttgttc
     6421 tgtttctgtt ttacttatgg gaatttcgct atttttagaa ttgcctgcaa tagtttccta
     6481 ctgggtagtt acagtgatgt gctttgtggc tatgggtgta tctactatgt ttgttgttca
     6541 aatttacact cttgttcaga cacaaacacc accgcaatta gtaggaaaga taatggctac
     6601 gctggtagca gttgctatgt gcggtcaacc tattggacaa atggtttacg gtattttatt
     6661 cgatgtattt atacaagaaa catgggttgt tatgtttgta gcttctattg cttcaatcat
     6721 gatttcaata tattcaaaaa gaatttttca aaagttagag atataataca aggggttaaa
     6781 tagtatgtta ggctaaaaac aatatataaa agaaaatttg aaatctgcta aaaggatagt
     6841 gttataaaag aaagagtaat attgcagtat ttaaggctcg cacaaatata tgttttgtgt
     6901 gggctttttt cttaccattt ttctgtattt ctaaagggta taaacggtat tccgtttata
     6961 tatttcagca ttagcagaaa gggggtgaga ttatgaggac atcttctttc gagcatatcg
     7021 tcagattaca gttcaattct ttaatgctga ttgtcattaa gaacaaagtg aaaagtcgaa
     7081 acagacagct tgcaagacat tctaaacgtg aagtattgtt ttgtgaaata gccgaaacga
     7141 aacaagttga acgtggaacg attgatacct gtttttatga ttgtgtatca tttaaagtct
     7201 tgcactttac aatttatgta tctgatgaaa cacttggtac agttttacat agactgtcag
     7261 aaaaacagcg tagtgcgatt ttattacgtt attttcaagg aatgaacgac agagaaattt
     7321 cagaactgta ccatgtatca cgttctgcta tttgcagtag aagaactaga ggactaagga
     7381 agttaaaaat gcttttgagc gaaaggaagt aaagaaatga gtaaagatta tggctatccc
     7441 tcttttgaat tgatttgcaa agcgtcaagc ggagatgaaa tggcagttaa agagattttg
     7501 aagttttatg acggttatat ttctaaatta tgcttgcgtc ctttttacca ctctgaaagt
     7561 ggtaaaatca ttatgcaggt tgatgaagaa ctaaaaggag aaattcatac agacatgatg
     7621 aaagcaatat tgaaatttga aatcggagtg aagtaatcaa ctgtataaaa aaatcgggag
     7681 atacacagtt tcgtgctatc tcccttagta gttttacggt catatcaagg ctcattcaat
     7741 cgtggtaaat tcgtggtaag atgagtgttt ttctatactt caaaatgctt gaatgtatag
     7801 tgtttatgcg gataattgaa aattagatat aaaatttcaa ataaattact gatataaatt
     7861 tacaataaga actcttgttt ctactttttt ttatgagaaa atttagtttt caatttattg
     7921 aaccattttc ggcttggatc tcgacgtttt ttgatttgat gacccgtcac gtattcttga
     7981 acaactgcat tgatgattcc gcccaaaatg atgatcgtag cagcaaaatt aagccaaagc
     8041 atgagtacaa taaaacttcc tatgatctga taaccgctga tcctcgttgc aaaataccta
     8101 gcatagatac taaacacttg tgataacagc atccagccga cagtagcgaa gatcgtgccc
     8161 ggaatgatcg tcttcaactt caactgagca ttagggacaa ttaaataaat caagaacatg
     8221 atgacaaata aagctaccaa tgtgattggc cacttcaaag tttggaactg atcgataaag
     8281 ttcacgggta tttgtacgat tggctgaata gcatccaaaa tcagctgtcc caacccaaga
     8341 acgatagtta ccccactaat agcaatcatg aacaagacaa tgacaattag tgagaagaaa
     8401 cggacgatca aaaaattttt tctattttct acgccatacg ctttattcat cgcaatttgc
     8461 aaagcattga tactttgact tgcagaccat aaagccgcta atgcagaaac ggataacaag
     8521 cctcctgaac tttgtgtcaa aaggtctttg attgcaggac cgataaactg gtaaacggtc
     8581 tcaggaatca cttctcgtaa ataggtcagt acagtctctt gatcgatgtg gagaaacggt
     8641 aaaatattac caaaagcaat aatcaatgga aataaagaca agagtaaata ataagcaacg
     8701 acgacagagg tcgtactcat ttctgaatca gtgatgcgct tttgagtcgt ctcaataaag
     8761 cgcatcagat tttcattttg ttttattttg ttccaaactt tcatgtacct ctcccgtacc
     8821 tattgactgg atagatcaat aagtgccttc ttctccttgt gatgtcaaaa ttcttggacc
     8881 atctttagta atagccaatg tatgttcata ttggcaagaa agtccgccgt ctcttgtata
     8941 cgcagtccag ccgttaggat ccattttcat tttccaagtt ccggtattaa ccataggttc
     9001 gatcgtgata accatgcctt ctttcaaccg caaccctttt ccagcttctc cgtaatgagg
     9061 aactgctggt tcttcatgaa tcgtaggacc aacgccatga ccaataaaat cacgaacgat
     9121 cgctagattt tcgccttcta cataagtttg gatagcatga ccgatgtcac caatccgatt
     9181 ccctacttgt gcttgctcaa taccgatgta caaagctttc ttcgttactt ccatcaatcg
     9241 gtcgatctca ggtgtagatt ctccaactac ataggcccaa caagaatcag aaatgccgcc
     9301 tttcaaatcg atacacatat cgacttttac caaatcaccg tttttcaaag gctttttacg
     9361 agggaatcca tgacaaattt catcatttat actgatacaa gtcgcatatt catacccttc
     9421 aaagccaatt tgcgcagcaa ttccgccatg actctcgata tagtttctga caaatacctc
     9481 aatatcccaa cttgtgattc ctggttttat aaaatcacgc agatttttat gtacatctgc
     9541 taataaagct ccagattctg ccatcatttc aatttcacgt gctgatttta aagtaatcat
     9601 attttcgtct cctttttaca aatccctctc attttaacat agcggcatac atttagccaa
     9661 atgacattcc ccaggtgtct ctaggtttta ttttcttttc aatgatgttt tggtataatt
     9721 cataattgtt aaataattat aaacgaggag gcacatcatg gctttagcaa aaatcgtata
     9781 tgctagcatg acaggaaaca ctgaagaaat cgccgatatt gttgcagaag catttgaaaa
     9841 tctggatatc gaagtcgaaa tcaacgaatg tacccaagta gatgcagatg aattcgagga
     9901 ggcagatatt tgcgtagtag ctacttatac atacgatgat ggcgatctgc ctgatgagat
     9961 cgtagacttt tatgaagact tgcaagaatt ggatctattg ggaaaaattt acggtgtctg
    10021 cggatctggt gatacttttt acgatgaatt ctgcaaatcg gtggatgatt tcgatgctgc
    10081 ttttgcaaaa acaggcgcat caaaaggagc cgaaaatgtt aaagtggatc tgaatgctga
    10141 agaagaagat atcgaaaatt tagaagcttt cgctaaagaa ctagtcgcaa aaatttaata
    10201 tacttgcaat aaaagagcca tccagattta ttcaatctgt atggctcttt tattcattgc
    10261 attcatggaa gagttcttcg ggagttaaaa atgcatacgc tgtaagaaaa acgaactttt
    10321 ccttattttg acacatctct tgttacctac tgatttgtgg aacttttgag tatattcaat
    10381 cgatttcgat tggttctttg aatttcaaga tagcatctat actgtcttgg atcatctttt
    10441 caatctgttc atcagttgga tggatgatgc ttgaagacat caaagctgcg atcccagtag
    10501 ctatgatcca tgtcccatta tacaaagatt cgatctgctc ttctgaaaga ttcacataag
    10561 ctggatcgcg tttcactgta ttcacaaagt aagtataaga gaaattctgc atctcttttc
    10621 ctccaccata ttcttctaag tacagtgaac gatacaactt cttctctctt cttgcaaaat
    10681 ggatataatt aaaagcaaga tcgataatcg tattccctgt atgctcaaca gtgaacactt
    10741 catattgtaa atgatcatag atctgctcaa aaagagcttg tctcaagtca tccatatttt
    10801 taaactcaag ataaatcggt tgcgtggaac aattcatttt gttcgcgatg tttctcgcag
    10861 taaatcgtgt aaatccatcg gtggcagcta cttcataagc agctgccaaa atctgttctc
    10921 tagtaatcgt cttttttctt gccatatttt ttaatctcct taattttttt ataacgcttc
    10981 caccattgat taatttcatt gtaacatgtt ttttttgata tgcaaaatta gtttttgatt
    11041 ttcttcacaa gctgaaatag caagtagtaa catatatcat gaaacttaac cttaaaaaaa
    11101 taacctcctt tttttattta aagcctcatt gtttgaatca taattaaatg gtatcatgct
    11161 aatagttacc agaaaaaatg gtgaggagga atttttatta tgttatctga ttttaatgaa
    11221 cttctaaaaa aatatgcacg cttaattgca gaaactggcg tagccacaga aaaaggacac
    11281 acagttgttt tacagatcag cgtggaacag gcaccccttg ctcgattgat cacacaagaa
    11341 gcttataaac taggagccgc tgaggttatc gttcaatgga ctgacgacca gatccaaaga
    11401 gaatttcttt tacatgctgc aacggatcgt attgagaatg taccacaatc taaaatcgat
    11461 caagcggatg aatggcttga aaaaggagca agcaggatca gcgttgtttc tgccgatccc
    11521 gatgcatttg ctggtgtaga tagccatcga gtagcgtctt atcaatcagc gaatggcaaa
    11581 gcactaatga atttacgcaa ggcaactcaa gcaaataaag tcagttggac tgtcgtggct
    11641 gcggctggta aacaatgggc agcaaaagtc ttcccagaac tgccagaaga aaaacaagtt
    11701 gaggcattat gggatcagat tttcaaaacg actcgtgtct atgaagaaga ccctgtctta
    11761 gcatggaaga agcatgatga aaaattagca aaaaaagcag aagaattgaa tcgagaacaa
    11821 ttttctgccc ttcactatac tgctcccggt acagatatca ttatcggatt gcctaaaaac
    11881 catctttggg aaggcgctgg aagctataat gctcgtggag aaaaattcat ggcaaatatg
    11941 ccgacggaag aagtgttcac agcacctgac agtcatcgag tagatggtta catttcaagt
    12001 acaaagccac tgagttatgc tggcaccatc atctcgggta tgaaatttac ttttaaagat
    12061 ggaaaagttg ttgatttttc tgccgaacaa ggagaagatg tcttagcaaa actgcttgat
    12121 acagatgaag gtgcgcgtcg tttaggagaa gtcgcacttg tacctgaccc ttcacctatt
    12181 tctcaatctg gcatcatctt tttcaataca ttatttgatg aaaacgcatc gaatcactta
    12241 gcactaggtt ctgcttatgc cttcagtgtg aaaggcggta cagaaatgtc cgatgaagaa
    12301 ttagcagaag ctggattgaa tagaagtcaa acccacgtag atttcatggt aggatctgac
    12361 aagatggata tcgacggaat ccgtgaagat ggctctacag taccgatttt ccgaaatggc
    12421 gattgggctt gagtatactt tcgcacacat gcaaaaaata atggctcttt gtcaataagg
    12481 actgatgagt tgtgcaaaat c
//
LOCUS       DAZKSR010000275         9496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.107, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000275 DAZKSR010000000
VERSION     DAZKSR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.107"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..528)
                     /locus_tag="ACG8XG_003168"
     CDS             complement(<1..528)
                     /locus_tag="ACG8XG_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0774980.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLA"
     gene            656..1225
                     /locus_tag="ACG8XG_003169"
     CDS             656..1225
                     /locus_tag="ACG8XG_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0774981.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1292..2029
                     /locus_tag="ACG8XG_003170"
     CDS             1292..2029
                     /locus_tag="ACG8XG_003170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0774982.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2260..3528
                     /locus_tag="ACG8XG_003171"
     CDS             2260..3528
                     /locus_tag="ACG8XG_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0774983.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4015..4479
                     /locus_tag="ACG8XG_003172"
     CDS             4015..4479
                     /locus_tag="ACG8XG_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0774984.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4463..5092
                     /locus_tag="ACG8XG_003173"
                     /pseudo
     CDS             4463..5092
                     /locus_tag="ACG8XG_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            5446..6924
                     /gene="msr(C)"
                     /locus_tag="ACG8XG_003174"
     CDS             5446..6924
                     /gene="msr(C)"
                     /locus_tag="ACG8XG_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0774985.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6971..7417
                     /locus_tag="ACG8XG_003175"
     CDS             6971..7417
                     /locus_tag="ACG8XG_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0774986.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7616..8131)
                     /locus_tag="ACG8XG_003176"
     CDS             complement(7616..8131)
                     /locus_tag="ACG8XG_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0774987.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8248..9450
                     /locus_tag="ACG8XG_003177"
     CDS             8248..9450
                     /locus_tag="ACG8XG_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0774988.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3025 a         1667 c         1977 g         2827 t
ORIGIN      
        1 tgctaaagga tgatttcgat gtatcaaaag tccccaacga tcttgggctg gcagtcgaaa
       61 tgactggtat ttttgtttgt ttgtcggttc gattacgagt ccaaaatcaa gtaatccgct
      121 atccagtttg tctatgatat catctgcatt cccgctatat aaatgaaact ggatatcagg
      181 atgcgccttg atcaacgaat gaaggctatc agaaatgaaa gagattgctt tagtttcgcc
      241 tccgccaatg tagatttctc cagagatgat tgcgtcattt gttaaattgt tcgccgtttt
      301 ttctaccaac gacaagattt cttttccttt tgaataaaga tatcgaccag aatccgtcaa
      361 ctgtatctct cgggagcccc gagtgaaaag tgtcacacca agttcttctt ctaaatcttt
      421 cagttgtttt gagagagtag gttgcgtgat atgcagagat tttgctgctc ggctgatatt
      481 tttttctcta gctactgcta aaaaatacgt cagtatacga atatccattt tttccctcct
      541 gtttttaaaa aagcatacca ctaagtatag ttaaaaggaa tagttaagta tgttttataa
      601 gtattagaca tgataagccg tccgttataa agtaaggata aaaggaggga gcaaaatgac
      661 aagcctcatc gaaaaacaaa tcgtcaataa agagattcga gtaacggatc cgctctttga
      721 agtgatccat cagatccaag ctgaaaacga agagccttta gcagcgctga atactgggta
      781 ccatgaacca gcagatatcc gcaaacggct agaaaatatc attagtgaca aagtagatga
      841 tacggtgact gttctgctgc ctttttatac agatttcggc aaacatatta gtatagggaa
      901 aaatgtcttt atcaatcgtc aagtgatgtt cgttgactta ggtggcatct gtttagaaga
      961 ttcagtattg attggtcctc gtgtcaacct catcacggtg aaccatctaa ccgatccaaa
     1021 agaacgaaga ggcttgttag taaagccaat ccacatcaag aaaaatgctt ggatcggagc
     1081 aggagcaact attctgccag gcgtaacgat tggcgagaat gccattgttg cagcgaatgc
     1141 aactgttacg aaagatgtcc cagacaatac catcgttgca ggaatccctg ccaaaatcgt
     1201 caaacctgtc gaacggatta tttgaacagt aagatgaaat aagagatagt gagtataagc
     1261 aagagatttt tcaaaacagg aggagaatag aatgaatgat ttatcagaaa aagtgattgt
     1321 catcatgggc gcttcaagcg gaatcggtga agcaacaacg aagctattag cagaaaaagg
     1381 ggctaaacta gttatagctg cacgccgaga agatcgccta aaagcaataa aagaatcttt
     1441 accagaagct gagctatata ttcaaacagc agatgtaaga gattttgcac aagtccaagc
     1501 agttatcgat ttagcgatgg aaaagttcgg tcgaatcgat gtattgtaca ataatgcagg
     1561 aatcatgcca accgctccat tagttgaagg tcaccgtgat gaatggcaaa atatgcttga
     1621 tatcaacatc atgggtgtgt taaatggaat ttccgccgta cttccaatca tggaaaaaca
     1681 aaaatctggg catatcattt caactgattc tgtggcggga catgttgttt atcctgattc
     1741 cgccgtttat tgcggaacaa aatttgctgt ccgtgcaatc atggaaggat tacgtcaaga
     1801 gcaacggcaa aataacatca aatctacgat catctctcca ggagctgttc aaactgaatt
     1861 atatcaaacc atcagcgaca aaaaggctgc tcttgaactt catgaagcgc aaaaagaatg
     1921 gggattgacc tccgaagata tagcttctgc tgtagcattt gcgatagaaa cgccagaccg
     1981 gatgtctgtc agtaacatga ttatccgtcc gacgacacag gaagtataat ccataacgga
     2041 taatatccaa agagaattac ttgttaaata gttattcaaa cgaccatcaa tttaaggcgt
     2101 gaatggagaa gaatattctt catttacgtc tttttatgta gaaaaagcaa atttatcaaa
     2161 aagaaacaaa tgttatttaa atatggacat tgacttgctt ttactttttt agttacactg
     2221 ttatttagga aaagtttcat aattcaaagg agaaaaaaga tgaaagcaaa gaatttatta
     2281 tttagcgtta ttcttttagg aaatctagca gttgccaaca caacagtgct agctgaagaa
     2341 tcgacagaaa ctgtcgcaac tcagacatcg agttcttcag aaacaggaat tgaaaacgat
     2401 acagagagta ctttgcagtc agctactgtc tcagaaaata caatttcaac cgaagaacca
     2461 acaactgaaa caacagaaca aacacaaacc gaagacagta catcagcaga agctcagaac
     2521 aatccagaga atgcagaaga aatcacgaac caattgacag atgggcaaac attgacacat
     2581 acccatcaag cagatttgtc tgaattcgaa aactatactg gctatgcttt ttatgctcgc
     2641 tcatctgcta attctcaaca agcgttcatt gatagtattg cctcaactgc acaaagtttg
     2701 gcaagtgcca atgacttata tgcatcagtc atgattgctc aagccatcgt tgaaagtggc
     2761 tgggggaaca gtactttggc ttctgcccct aattacaatc tatttgggat taaaggaagc
     2821 tataatggcc aatctgttac gatgcctact tcggaatatg tgaatgggca atggattacg
     2881 gtgaatgccg cttttagaaa atatccttcg tacaaagaat cattgcaaga taacgtaacg
     2941 gtattgaaga cgacttcttt tcagccaggc gtttactact attccggtgc atggaaaagc
     3001 aatacgaact cttacaaaga tgctacggct tggctaacag gtcgttatgc cactgcgcct
     3061 aattatggtt ctacattgaa caatgtaatc gaaacctata atctgacaca gtatgataca
     3121 gcaccaactt caacttctcc gatgtatcgt ctgtataacc gtcatacagg agaacattta
     3181 tatacattga atgccggaga aaaaaattat ctgccaactg taggctggga atatgaaggg
     3241 attgcttggc aagcaccaaa cagcggacaa cctgtctacc gtttatacaa tccttacagt
     3301 ggagaccatc attacacaat ggcacaatct gagatcaact ctttaacaaa aatcggttgg
     3361 cgttatgaag gtctaagttt ttattctggt ggttccaagc caatttatcg actatttaat
     3421 ccaaatgaaa aaactggtac ccatcactat accttgagtg ctaaggaaag agattggcta
     3481 acaccgatgg gctggagata tgaaggaata ggattttatg gttattaaaa agaggtagtg
     3541 aaaaagctgc cctgcatcaa gtagtaagaa cagccaaaca aaaacagatt ttcatgtttt
     3601 ttgtttggtt gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag
     3661 gttgtgacaa catcttgtca taactttttt attactgttt aagcatactg atccaatttc
     3721 taatattcga cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaattt
     3781 tctactgcag aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg
     3841 ttgtgctttc tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt
     3901 ttctattgag agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat
     3961 tctattattg acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct
     4021 ttagaagaga ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag
     4081 atctttgaag aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa
     4141 gaagaaaatc cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca
     4201 gttactcgtc atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct
     4261 cagaacaacc gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcgagcat
     4321 tgtgaaatgg accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa
     4381 atgaaacaat ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca
     4441 gaaacgaaag aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg
     4501 tagaagatcc gttcgcgtat atgatgaaac atacaaaatc ccacacgaat aaatgctgga
     4561 aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt
     4621 ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag
     4681 acaaaatgat acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata
     4741 tggcgaagaa atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga
     4801 tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga
     4861 tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca
     4921 tggctatgaa acaaatccaa ttggtggagt cgaagcagat cagttggcag aggcatttgg
     4981 tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga
     5041 aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt
     5101 cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag
     5161 gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa
     5221 ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg
     5281 aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg
     5341 ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt
     5401 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
     5461 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
     5521 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
     5581 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
     5641 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
     5701 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
     5761 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
     5821 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
     5881 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
     5941 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
     6001 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
     6061 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
     6121 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc
     6181 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
     6241 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
     6301 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
     6361 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
     6421 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
     6481 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
     6541 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
     6601 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
     6661 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
     6721 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
     6781 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
     6841 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
     6901 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
     6961 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
     7021 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
     7081 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
     7141 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
     7201 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
     7261 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
     7321 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
     7381 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
     7441 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
     7501 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
     7561 tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact
     7621 caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa
     7681 gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga
     7741 ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga
     7801 atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa
     7861 attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac
     7921 gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg
     7981 tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt
     8041 cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca
     8101 cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt
     8161 tattatatca cagtaaagtt tccacgtttt acatgtgcat atttacatgt aaaataaatc
     8221 atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa
     8281 gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt
     8341 atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag
     8401 ttaaacgcta cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt
     8461 gtggtgatgc tatttacagg tttcttatct agtcggatcg gtttgaaaaa aacattgatg
     8521 acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga
     8581 atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt ctctactgct
     8641 ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat gatgtatgaa
     8701 gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt gggcagtggt
     8761 tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt tatagcccta
     8821 gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg ggaaggtgtc
     8881 aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt atataatttc
     8941 ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc tgaattagaa
     9001 gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat tttcgttgca
     9061 ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt tgtcttattc
     9121 attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt accgatcagc
     9181 attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac gattgctatg
     9241 gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt ccgctttttc
     9301 ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg tgaaagctat
     9361 gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat ttatcaccgt
     9421 aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa agctgtcctg
     9481 catcaagtca taagaa
//
LOCUS       DAZKSR010000276         7860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.119, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000276 DAZKSR010000000
VERSION     DAZKSR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.119"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8XG_003178"
     CDS             <1..734
                     /locus_tag="ACG8XG_003178"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0774989.1"
                     /translation="IVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRR
                     NVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFW
                     EVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRID
                     QQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAM
                     EHRQTLEKTKILGQTLSRFFTILPK"
     gene            992..2494
                     /gene="eat(A)"
                     /locus_tag="ACG8XG_003179"
     CDS             992..2494
                     /gene="eat(A)"
                     /locus_tag="ACG8XG_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0774990.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2561..3088
                     /locus_tag="ACG8XG_003180"
     CDS             2561..3088
                     /locus_tag="ACG8XG_003180"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0774991.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3299..4360
                     /locus_tag="ACG8XG_003181"
     CDS             3299..4360
                     /locus_tag="ACG8XG_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0774992.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4363..5259
                     /gene="murQ"
                     /locus_tag="ACG8XG_003182"
     CDS             4363..5259
                     /gene="murQ"
                     /locus_tag="ACG8XG_003182"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0774993.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5273..7255
                     /locus_tag="ACG8XG_003183"
     CDS             5273..7255
                     /locus_tag="ACG8XG_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0774994.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7320..7766)
                     /gene="nrdI"
                     /locus_tag="ACG8XG_003184"
     CDS             complement(7320..7766)
                     /gene="nrdI"
                     /locus_tag="ACG8XG_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0774995.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         2595 a         1374 c         1759 g         2132 t
ORIGIN      
        1 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
       61 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
      121 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
      181 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
      241 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
      301 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
      361 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
      421 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
      481 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
      541 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
      601 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
      661 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
      721 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
      781 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
      841 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
      901 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
      961 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     1021 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     1081 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     1141 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     1201 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     1261 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     1321 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     1381 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     1441 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     1501 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     1561 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     1621 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     1681 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     1741 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     1801 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     1861 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     1921 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     1981 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     2041 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     2101 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     2161 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     2221 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     2281 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     2341 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     2401 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     2461 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     2521 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     2581 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     2641 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     2701 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     2761 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     2821 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     2881 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     2941 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     3001 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     3061 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     3121 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     3181 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     3241 ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga gggataatat
     3301 gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg
     3361 ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga
     3421 tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact
     3481 agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga
     3541 tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa
     3601 tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac
     3661 gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg
     3721 gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa
     3781 tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt
     3841 gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct
     3901 tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg
     3961 tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct
     4021 ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg
     4081 gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc
     4141 tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa
     4201 gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa
     4261 agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc
     4321 tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta
     4381 gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt
     4441 tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa
     4501 gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga
     4561 cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa
     4621 tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa
     4681 gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat
     4741 ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt
     4801 acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg
     4861 atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat
     4921 gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt
     4981 atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg
     5041 atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg
     5101 caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat
     5161 gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa
     5221 ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa
     5281 aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg
     5341 caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga
     5401 tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga
     5461 acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga
     5521 tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc
     5581 atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa
     5641 atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt
     5701 gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc
     5761 aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat
     5821 gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc
     5881 acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac
     5941 agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg
     6001 acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa
     6061 actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat
     6121 tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg
     6181 catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct
     6241 aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca
     6301 tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg
     6361 tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt
     6421 aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat
     6481 ttacggagta acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat
     6541 cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg
     6601 agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt
     6661 ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt
     6721 agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt
     6781 acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt
     6841 ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt
     6901 tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt
     6961 cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat
     7021 gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca
     7081 gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg
     7141 caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt
     7201 ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga
     7261 tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat
     7321 tatttattgt tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt
     7381 aattcataat ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga
     7441 caatactgat tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga
     7501 ttgtcttcat gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta
     7561 tctaaaccat ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
     7621 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
     7681 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct
     7741 gaaatcgata tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc
     7801 atctttttta agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
//
LOCUS       DAZKSR010000277         5851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.146, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000277 DAZKSR010000000
VERSION     DAZKSR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.146"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1014
                     /locus_tag="ACG8XG_003185"
     CDS             322..1014
                     /locus_tag="ACG8XG_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0774996.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG8XG_003186"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="ACG8XG_003186"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0774997.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="ACG8XG_003187"
     CDS             complement(1910..3862)
                     /locus_tag="ACG8XG_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0774998.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="ACG8XG_003188"
     CDS             complement(3869..4105)
                     /locus_tag="ACG8XG_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0774999.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="ACG8XG_003189"
     CDS             4507..4797
                     /locus_tag="ACG8XG_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0775000.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(5069..5418)
                     /locus_tag="ACG8XG_003190"
                     /pseudo
     CDS             complement(5069..5418)
                     /locus_tag="ACG8XG_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5511..5813
                     /locus_tag="ACG8XG_003191"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="ACG8XG_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1997 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DAZKSR010000278         5445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000278 DAZKSR010000000
VERSION     DAZKSR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.149"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1687
                     /locus_tag="ACG8XG_003192"
     CDS             <1..1687
                     /locus_tag="ACG8XG_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0775001.1"
                     /translation="SQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPANLEKPVP
                     DTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVDQSDEYK
                     KIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALAL
                     AAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRV
                     QTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNG
                     IPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTK
                     HPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGI
                     IKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDH
                     EVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISL
                     LQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            1917..2984
                     /gene="rlmN"
                     /locus_tag="ACG8XG_003193"
     CDS             1917..2984
                     /gene="rlmN"
                     /locus_tag="ACG8XG_003193"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0775002.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            2974..3180
                     /locus_tag="ACG8XG_003194"
     CDS             2974..3180
                     /locus_tag="ACG8XG_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0775003.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(3177..4280)
                     /locus_tag="ACG8XG_003195"
     CDS             complement(3177..4280)
                     /locus_tag="ACG8XG_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0775004.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4832..5380
                     /gene="aac(6')"
                     /locus_tag="ACG8XG_003196"
     CDS             4832..5380
                     /gene="aac(6')"
                     /locus_tag="ACG8XG_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0775005.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1812 a          949 c         1158 g         1526 t
ORIGIN      
        1 gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga
       61 gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga
      121 gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt
      181 gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga
      241 ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga
      301 gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt
      361 cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc
      421 ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt
      481 agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga
      541 agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa
      601 ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac
      661 ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc
      721 agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa
      781 actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct
      841 gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca
      901 agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta
      961 caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt
     1021 catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga
     1081 agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc
     1141 tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag
     1201 aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc
     1261 agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat
     1321 ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac
     1381 agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac
     1441 aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga
     1501 aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc
     1561 agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa
     1621 gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga
     1681 catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat
     1741 gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg
     1801 tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa
     1861 ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg
     1921 aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg
     1981 gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta
     2041 atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta
     2101 tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt
     2161 tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct
     2221 tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg
     2281 ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg
     2341 tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg
     2401 ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg
     2461 atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac
     2521 acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg
     2581 cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga
     2641 aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat
     2701 atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat
     2761 taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac
     2821 atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga
     2881 aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg
     2941 gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt
     3001 ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag
     3061 agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag
     3121 caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa
     3181 ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga
     3241 atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt
     3301 gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt
     3361 aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac
     3421 tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat
     3481 ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta
     3541 tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact
     3601 tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa
     3661 gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct
     3721 agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata
     3781 cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt
     3841 acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca
     3901 cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta
     3961 gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac
     4021 aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt
     4081 gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt
     4141 attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca
     4201 tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt
     4261 aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt
     4321 taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat
     4381 aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa
     4441 caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc
     4501 gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa
     4561 aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc
     4621 atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg
     4681 aataaagtgt ttctgtataa ttttttattt tataagtaag ataaataatg aaagaataga
     4741 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
     4801 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
     4861 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
     4921 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
     4981 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
     5041 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
     5101 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
     5161 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
     5221 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
     5281 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
     5341 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
     5401 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tactt
//
LOCUS       DAZKSR010000279         4804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000279 DAZKSR010000000
VERSION     DAZKSR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.164"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8XG_003197"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8XG_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0775006.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ACG8XG_003198"
     CDS             765..1919
                     /locus_tag="ACG8XG_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0775007.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="ACG8XG_003199"
     CDS             2134..3102
                     /locus_tag="ACG8XG_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0775008.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8XG_003200"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8XG_003200"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0775009.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="ACG8XG_003201"
     CDS             4132..4740
                     /locus_tag="ACG8XG_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0775010.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1281 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccct
//
LOCUS       DAZKSR010000280         3843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.183, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000280 DAZKSR010000000
VERSION     DAZKSR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.183"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..998)
                     /locus_tag="ACG8XG_003202"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="ACG8XG_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="ACG8XG_003203"
     CDS             1489..2427
                     /locus_tag="ACG8XG_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0775011.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="ACG8XG_003204"
     CDS             2557..3204
                     /locus_tag="ACG8XG_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0775012.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1303 a          552 c          643 g         1345 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat aaagtccata taattgtgta aaagataaaa
     3841 ggc
//
LOCUS       DAZKSR010000281         3686 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.185, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000281 DAZKSR010000000
VERSION     DAZKSR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.185"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..230
                     /locus_tag="ACG8XG_003205"
     CDS             <1..230
                     /locus_tag="ACG8XG_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0775013.1"
                     /translation="DFILYLKLCNRTLLVEDASLSGENERINELIEFIRKNKFRTLEY
                     DDKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            373..597
                     /locus_tag="ACG8XG_003206"
     CDS             373..597
                     /locus_tag="ACG8XG_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0775014.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            612..1481
                     /locus_tag="ACG8XG_003207"
     CDS             612..1481
                     /locus_tag="ACG8XG_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0775015.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1462..2196
                     /locus_tag="ACG8XG_003208"
     CDS             1462..2196
                     /locus_tag="ACG8XG_003208"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0775016.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2229..3137
                     /locus_tag="ACG8XG_003209"
     CDS             2229..3137
                     /locus_tag="ACG8XG_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0775017.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3134..3614
                     /locus_tag="ACG8XG_003210"
                     /pseudo
     CDS             3134..3614
                     /locus_tag="ACG8XG_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1279 a          533 c          801 g         1073 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct tttagtagaa gatgcctccc
       61 tcagtggcga gaacgagcga atcaatgagc tgattgaatt tatccgcaag aacaaattcc
      121 gcaccttaga atatgatgat aagcttgtaa ggaagataat ccagagcgtt acagtctatg
      181 aaggccactt cgtcatagcc ttcaaatctg gcatcgaaat ggaaatatga aaaccagaag
      241 taaatagccc atgactctac agtagagttg tgggttttct tttgcttgct gattataata
      301 atattttaat aactattgtg tttatcaacc aaatggttta taatattctt agcgtaaact
      361 tggaggtgct gtatgactac ggcagaaatg attaaagaac tgtgtgagca aatgaatata
      421 agtgtttccg aacttgctag acgtattggc cagactccac agaatttcaa taaaaaatta
      481 caacgagaaa cagtaacctt ggatgagttg aaggccattg ctgatgtgct aggtgtcaag
      541 tttgtgcagg catttatttt accagatgga gaagaaataa aaatatctaa cgaataaagg
      601 aggatgttta tatggtaggt aatgttattc aaatagtaac agagaaatta tcatctttgc
      661 cttttataga aggcatcgta ttagggggct cacgtgcaag aggcacccat acagaaaatt
      721 ctgatataga tatcggaatc tattacaatt cagactcatt tgacctgaca gcgattaatc
      781 aaattgctac agaattggat gataaaaata gaaacaacct tgttgtgcct cccggagcgt
      841 ggggtgattg gattaatggc ggcggatggt tatttataaa tgggtatcat gttgacttga
      901 ttttacgtga tataaaacgg gtagaacaaa taatcaaaga tacggagcaa ggaattgtta
      961 ctgccaatta tcagaccggg catccccatg gttatataag tgcaatgtat cgtggagaat
     1021 tagcgattag taaaatacta tatgctaaga acgaaagctt atgcgaatta aaaaagcagg
     1081 cagaaattta ccctactgct ctaaagaaaa gcttgatgaa cttttttata tttgaagctg
     1141 aattctcttt aatgtttgta aaagcaaatg cgggggtaga ggataaatat tatattgcag
     1201 gccatgtttt tcgtattatt tcatgcttaa atcaagtact atttgcatgt aataatgctt
     1261 attgcattaa cgaaaagaaa gctataaaac tgcttgaaac ttttgaatat aaacctgaaa
     1321 aatatgccga aagagttaat catatttttg aagtactcgg tctttctctt tttgaatgct
     1381 acgatatgac cgagaaactt tataaagaag tgaatgaaat tgtatcggag ataaataact
     1441 ttttaaacga ggagagttca gatgaaagaa aacaaatatg atgataatat attttttcaa
     1501 aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact
     1561 ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat
     1621 ggatggcact gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt
     1681 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
     1741 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
     1801 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
     1861 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
     1921 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
     1981 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
     2041 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
     2101 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
     2161 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
     2221 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
     2281 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
     2341 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
     2401 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
     2461 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
     2521 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
     2581 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     2641 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     2701 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     2761 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     2821 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     2881 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     2941 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     3001 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     3061 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     3121 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     3181 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     3241 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     3301 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     3361 tttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc
     3421 ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa
     3481 cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct
     3541 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
     3601 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtgg ttctgttgca
     3661 aagttttaaa taaagaataa aatccc
//
LOCUS       DAZKSR010000282         3195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000282 DAZKSR010000000
VERSION     DAZKSR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.192"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8XG_003211"
     CDS             260..1171
                     /locus_tag="ACG8XG_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0775018.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8XG_003212"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG8XG_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0775019.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ACG8XG_003213"
     CDS             2328..2612
                     /locus_tag="ACG8XG_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0775020.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..>3195
                     /locus_tag="ACG8XG_003214"
     CDS             2978..>3195
                     /locus_tag="ACG8XG_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0775021.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGK"
BASE COUNT         1193 a          448 c          587 g          967 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaa
//
LOCUS       DAZKSR010000283         2827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.202, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000283 DAZKSR010000000
VERSION     DAZKSR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.202"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1348
                     /locus_tag="ACG8XG_003215"
     CDS             <1..1348
                     /locus_tag="ACG8XG_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFQ0775022.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..2650)
                     /locus_tag="ACG8XG_003216"
     CDS             complement(1478..2650)
                     /locus_tag="ACG8XG_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFQ0775023.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          991 a          360 c          494 g          982 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta gtcgattgtg ggaatttcct tgtacggtat
     1681 attgaaaggc gtcttcgaat ccatcatcca atgatgcgca agcttttgaa tattttggtt
     1741 gatcgatata atcatgaatc aatcgatttt tagcctcacg cgctaagtta atatctgtga
     1801 acttaaaaat tcctttaaca gcttctctga aagattttga atttttttta ggaatggtgg
     1861 taaagatatt tcttaggaag tgaacttggc atctttgcca acttacgttg gtgaaggatt
     1921 ttctaatggc agagactaat cctttgtgcg catcagaaat aacgagttcc gtaccttgta
     1981 aaccgcgttc ttttaggtat tcaaaaaatg ttgtccaggt ctcttcgctt tcgccacttt
     2041 gaatcatgaa gccgataatt tcacggtcgc catctttggt tattccaatc gctatatgac
     2101 agctttttga gagtactcga ttttcttctc gtacttttat atagagtaca tcggtcatta
     2161 agtaaggata atttttttct gataataaac gattctgcca ctcgttaacc ataggttcta
     2221 gctgttctgt taagctagaa acgaaggact tagagacgga tttaccacaa agttcttcca
     2281 caatttttga tactttacga gttgaaacgc ctgatacata catttccaac attgaagcca
     2341 tgagggcttt ttcgtttcgt tgataacgtt caaacactgt gggtgaaaaa tggccatcac
     2401 gtgttctggg tacttttaat tctagcgtgc ctacacgtgt cgtaaagctg cgctcataat
     2461 agccatttcg ttgactttgt cggttttctg ttcgttcata ttcttttgct tgaatatatt
     2521 ctgttcgttg attttccatt agttgattaa ataccgttgt taaaatattt ttagaaacgt
     2581 catcctttac agaatattca ataatgcttt gaatctcttc gcttttcagt gtaaaatgta
     2641 cttgggtcat gtaaaagtcc tcctgggtat gtttttgtcg ttaaaaacat tgtaccgtaa
     2701 aaggactgtt atatggcctt tttactttta cacaattata cggactttat ctatataaag
     2761 gcgctgtcag agttttttga cttagaacat acattcattc aagagacttt aaacaagcta
     2821 gcagaac
//
LOCUS       DAZKSR010000284         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.233, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000284 DAZKSR010000000
VERSION     DAZKSR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.233"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..73
                     /locus_tag="ACG8XG_003217"
     CDS             23..73
                     /locus_tag="ACG8XG_003217"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0775024.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            198..935
                     /gene="erm(B)"
                     /locus_tag="ACG8XG_003218"
     CDS             198..935
                     /gene="erm(B)"
                     /locus_tag="ACG8XG_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0775025.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          420 a          199 c          184 g          342 t
ORIGIN      
        1 gccttttatc ttttacacaa ttatatggac tttatcgaaa cagactaaaa acagtgatta
       61 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      121 acagataact aaaattacaa acaaatcgtt taacttctgt atttgtttat agatgtaatc
      181 acttcaggag agattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      241 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      301 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      361 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaata
      421 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      481 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      541 ttgaaagccg tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      601 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      661 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      721 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      781 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      841 tgaaacacgc caaagtaaac aatttaagta ccattactta tgagcaagta ttgtctattt
      901 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt
      961 ggaaagttac acgttactaa agggaatgga gataaattat tagatatact actgacagct
     1021 tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa
     1081 attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt
     1141 gtatt
//
LOCUS       DAZKSR010000285         1117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3006
            SAMEA115706384-rid22574333.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAZKSR010000285 DAZKSR010000000
VERSION     DAZKSR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706384
            Sequence Read Archive: ERR13196120
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287635.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287635.1
            Genome Coverage       :: 162x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:26:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,219
            CDSs (total)                      :: 3,163
            Genes (coding)                    :: 3,073
            CDSs (with protein)               :: 3,073
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 39 of 90
            Pseudo Genes (incomplete)         :: 45 of 90
            Pseudo Genes (internal stop)      :: 23 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706384-rid22574333.guided.235"
                     /isolate="VRE3006"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            529..1024
                     /gene="dfrF"
                     /locus_tag="ACG8XG_003219"
                     /pseudo
     CDS             529..1024
                     /gene="dfrF"
                     /locus_tag="ACG8XG_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
BASE COUNT          386 a          132 c          249 g          350 t
ORIGIN      
        1 cctcaggctt cagcttagta tttttcttgt tattaggcgg tttgctatgg tttatccctg
       61 tggcattgtg cgcagcagag atggcaacgg tgaaaggatg ggaaaaaggt gtttcccaaa
      121 caaaaagata ttaagaaaca gcccatgttt acctgcttgc aaacaaaaga atattgctac
      181 tagactaaga gatactacat aagtgtgttg gtatctcttt tttgttgccg tgaaacagca
      241 gactatttca tttctgcttg attaccttaa aacttattag tctatggttt gtcaagggct
      301 tgttgcgtga gaaatgctga atatcaccct ttacaaaaca taggctgata agtaactttt
      361 caaaagcaga aaagaaatgg caatgcttat aacaatatga tataatgcag atgaagacaa
      421 aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa gatgtggtgc
      481 acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat gataggtttg
      541 attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc atggaaaata
      601 aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat tatggggcga
      661 aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt tgtttccacc
      721 acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga tgcattatta
      781 ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa ggaagctttg
      841 caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga tggagataca
      901 ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac acttggtgag
      961 gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta aattttggat
     1021 ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg taagacaaat
     1081 tccagtttgt atggggcgat aaaatcagaa tttgaga
//
